Biology Commons^™

Prevalence Of Zoonotic Parasites In Stray Dogs In Rural Communities, Tanzania, Winifrida Kidima

Tanzania Journal of Science

The aim of this study was to identify and establish the baseline information on the prevalence of zoonotic parasites in stray dogs in Tanzania. A cross sectional study was conducted in Katavi, Arusha and Rukwa Regions in Tanzania. Intestinal parasites were analyzed from faecal samples collected from 364 dogs using modified sedimentation technique. Sixty-sixpercentage (66%) of the faecal samples were positive for gastrointestinal parasites while 7% had mixed infections, 27% were negative for the parasites. Higher proportions of the faecal samples were positive for gastrointestinal parasites in Arusha and Rukwa compared to Katavi (p< 0.0001). The prevalence of Ancylostomacaninum(49%) was higher compared to that of other parasites (p <0.0001). Prevalence of A. lumbricoideswas significantly higher compared to that of E. granulosusand S. stercoralis(p = 0.0046). There was a positive association between parasite positivity rates and collecting dog’s faecal samples near domestic animals (p < 0.00001). Parasite diversity was higher in stray dogs from Katavi and Arusha (p > 0.05). There was no association between …

A High-Throughput Method For Unbiased Quantitation And Categorization Of Nuclear Morphology, Benjamin Matthew Skinner, Claudia Cattoni Rathje, Joanne Bacon, Emma Elizabeth Philippa Johnson, Erica Larson, Emily E K Kopania, Jeffrey Martin Good, Gullalaii Yousafzai, Nabeel Ahmed Affara, Peter James Ivor Ellis

Biological Sciences: Faculty Scholarship

The physical arrangement of chromatin in the nucleus is cell type and species-specific, a fact particularly evident in sperm, in which most of the cytoplasm has been lost. Analysis of the characteristic falciform (“hook shaped”) sperm in mice is important in studies of sperm development, hybrid sterility, infertility, and toxicology. However, quantification of sperm shape differences typically relies on subjective manual assessment, rendering comparisons within and between samples difficult.

We have developed an analysis program for morphometric analysis of asymmetric nuclei and characterized the sperm of mice from a range of inbred, outbred, and wild-derived mouse strains. We find that …

Automated Nuclear Cartography Reveals Conserved Sperm Chromosome Territory Localization Across 2 Million Years Of Mouse Evolution, Benjamin Matthew Skinner, Joanne Bacon, Claudia Cattoni Rathje, Erica Larson, Emily Emiko Konishi Kopania, Jeffrey Martin Good, Nabeel Ahmed Affara, Peter James Ivor Ellis

Biological Sciences: Faculty Scholarship

Measurements of nuclear organization in asymmetric nuclei in 2D images have traditionally been manual. This is exemplified by attempts to measure chromosome position in sperm samples, typically by dividing the nucleus into zones, and manually scoring which zone a fluorescence in-situ hybridisation (FISH) signal lies in. This is time consuming, limiting the number of nuclei that can be analyzed, and prone to subjectivity. We have developed a new approach for automated mapping of FISH signals in asymmetric nuclei, integrated into an existing image analysis tool for nuclear morphology. Automatic landmark detection defines equivalent structural regions in each nucleus, then dynamic …

Characterization Of Vps41 And Its Role In The Regulated Secretory Pathway, Christian Henry Burns

Electronic Theses and Dissertations

Insulin secretory granules (SGs) mediate the regulated secretion of insulin, which is essential for glucose homeostasis. The basic machinery responsible for this regulated exocytosis consists of specific membrane proteins present both at the plasma membrane and on insulin SGs. The protein composition of insulin SGs thus dictates their release properties, yet the mechanisms controlling insulin SG formation, which determines this molecular composition, remain poorly understood. VPS41, a component of the endolysosomal tethering HOPS complex, was recently identified as a cytosolic factor involved in the formation of neuroendocrine/neuronal granules. We now find that a stable pool of VPS41 exists outside of …

Qualitative And Quantitative Fitness Consequences Of Advanced Maternal Age, Claudia Jean Hallagan

Electronic Theses and Dissertations

Parental age can affect offspring fitness across taxa and through various mechanisms. However, the effect(s) of advanced maternal age on offspring, particularly in insects, has not been comprehensively reviewed making it difficult to draw conclusions about the effects of advanced maternal age on offspring in insects. In my first chapter, I reviewed maternal age literature and found overall negative effects of advanced maternal age on offspring fitness. However, results vary depending on which fitness measures were used, the life stages at which offspring were measured, and the experimental design of the study. In my second chapter, I conducted an experiment …

Tree Peony Species Are A Novel Resource For Production Of Α-Linolenic Acid, Aruna Kilaru, Lihang H. Xie, Qingyu Zhang, Yanlong Zhang

ETSU Faculty Works

Tree peony is known worldwide for its excellent ornamental and medical values, but recent reports that their seeds contain over 40% α-linolenic acid (ALA), an essential fatty acid for humans drew additional interest of biochemists. To understand the key factors that contribute to this rich accumulation of ALA, we carried out a comprehensive study of oil accumulation in developing seeds of nine wild tree peony species. The fatty acid content and composition was highly variable among the nine species; however, we selected a high- (P. rockii) and low-oil (P. lutea) accumulating species for a comparative transcriptome analysis. Similar to other …

Comparative Transcriptome Analysis Reveals An Efficient Mechanism For Α-Linolenic Acid Synthesis In Tree Peony Seeds, Qingyu Zhang, Rui Yu, Daoyang Sun, Mahbubur Rahman, Lihang Xie, Jiayuan Hu, Lixia He, Aruna Kilaru, Lixin Niu, Yanlong Zhang

ETSU Faculty Works

Tree peony (Paeonia section Moutan DC.) species are woody oil crops with high unsaturated fatty acid content, including α-linolenic acid (ALA/18:3; >40% of the total fatty acid). Comparative transcriptome analyses were carried out to uncover the underlying mechanisms responsible for high and low ALA content in the developing seeds of P. rockii and P. lutea, respectively. Expression analysis of acyl lipid metabolism genes revealed upregulation of select genes involved in plastidial fatty acid synthesis, acyl editing, desaturation, and triacylglycerol assembly in seeds of P. rockiirelative to P. lutea. Also, in association with ALA content in seeds, …

Establishment Of An Efficient In Vitro Propagation System For Iris Sanguinea, Ling Wang, Yu Du, Mahbubur Rahman, Biao Tang, Li-Juan Fan, Aruna Kilaru

ETSU Faculty Works

Iris sanguinea is a perennial flowering plant that is typically cultivated through seeds or bulbs. However, due to limitations in conventional propagation, an alternate regeneration system using seeds was developed. The protocol included optimization of sterilization, stratification and scarification methods as iris seeds exhibit physiological dormancy. In addition to chlorine-based disinfection, alkaline or heat treatment was used to break seed dormancy and reduce contamination. When seeds were soaked in water at 80 °C overnight, and sterilized with 75% EtOH for 30 s and 4% NaOCl solution for 20 minutes, contamination was reduced to 10% and a 73.3% germination was achieved. …

Spg Directs Arp2/3 Mediated F-Actin Networks To Support Syncytial Furrow Ingression In Drosophila, Shannon M. Henry

Electronic Theses and Dissertations

In the Drosophila embryo, nuclear divisions 10-13 occur in a syncytium with transient membrane furrows separating neighboring nuclei before the occurrence of cellularization. This process is driven by cytoskeletal and membrane trafficking networks, and while RalA and Rab8 have been identified to drive membrane addition to furrows, less is known about the control of dynamic F-actin networks needed for furrow formation. Here, the role of the DOCK protein Sponge (Spg) in furrow formation is explored through shRNA knockdown and live-imaging of syncytial Drosophila embryos. I have found that Spg is required for furrow ingression and that without Spg, furrows can …

In Silico Pharmacokinetic And Molecular Docking Studies Of N-Cinnamoyltetraketide Derivatives As Inhibitors Of Cyclooxygenase-2 Enzyme, Stephen S. Nyandoro, Daniel M. Shadrack, Joan J. E. Munissi, Egid B. Mubofu

Tanzania Journal of Science

Recent phytochemical analysis of Toussaintia orientalis leaves yielded series of novel bioactive N-cinnamoyltetraketide derivatives namely toussaintines A-G (t_1 - t_8) some portraying cytotoxicity against the triple negative aggressive human breast cancer cell line (MDA-MB-231) among other potencies. Despite having broad bioactivity spectrum, their general drug-likeness profiles and mode of action (simulated or actual) targeting any enzyme remains uninvestigated. In silico pharmacokinetic, drug-likeness descriptors and molecular docking of the compounds t_1-t_8 targeting inhibition of cyclooxygenase-2 (COX-2) enzyme were evaluated. The Lipinski Rule of Five heralded the pharmacokinetic properties of the studied metabolites. The studied compounds were docked with COX-2 following already …

Bioactivities Of Extracts, Debromolaurinterol And Fucosterol From Macroalgae Species, Sartaz Begum, Stephen S. Nyandoro, Amelia S. Buriyo, John J. Makangara, Joan J. E. Munissi, Sandra Duffy, Vicky M. Avery, Mate Erdelyi

Tanzania Journal of Science

Parasitic diseases including malaria, and other numerous microbial infections and physiological diseases are threatening the global population. Tanzanian coast shores are endowed with a variety of macroalgae (seaweeds), hitherto unsystematically explored to establish their biomedical potentials. Thus, antiplasmodial activity using malarial imaging assay, antimicrobial activity using microplate dilution technique, antioxidant activity using DPPH radical scavenging method and cytotoxicity using brine shrimp test were carried out on crude extracts from the selected species of algae (Acanthophora spicifera, Cystoseira myrica, Cystoseira trinodis, Laurencia filiformis, Padina boryana, Sargassum oligocystum, Turbinaria crateriformis, Ulva fasciata and Ulva reticulata) occurring along the coast of Tanzania. The …

In Silico Pharmacokinetic And Molecular Docking Studies Of N-Cinnamoyltetraketide Derivatives As Inhibitors Of Cyclooxygenase-2 Enzyme, Stephen S. Nyandoro, Daniel M. Shadrack, Joan J. E. Munissi, Egid B. Mubofu

Tanzania Journal of Science

Recent phytochemical analysis of Toussaintia orientalis leaves yielded series of novel bioactive N-cinnamoyltetraketide derivatives namely toussaintines A-G (t_1 - t_8) some portraying cytotoxicity against the triple negative aggressive human breast cancer cell line (MDA-MB-231) among other potencies. Despite having broad bioactivity spectrum, their general drug-likeness profiles and mode of action (simulated or actual) targeting any enzyme remains uninvestigated. In silico pharmacokinetic, drug-likeness descriptors and molecular docking of the compounds t_1-t_8 targeting inhibition of cyclooxygenase-2 (COX-2) enzyme were evaluated. The Lipinski Rule of Five heralded the pharmacokinetic properties of the studied metabolites. The studied compounds were docked with COX-2 following already …

In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto

Tanzania Journal of Science

Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA …

In Vivo Antimycobacterial Studies Of Toussaintine A-Chitosan Nanocomposites, Edward Rwegasila, Joan J.E. Munissi, Egid B. Mubofu, Stephen S. Nyandoro, Paul Erasto

Tanzania Journal of Science

Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA …

Vertex Sliding Drives Intercalation By Radial Coupling Of Adhesion And Actomyosin Networks During Drosophila Germband Extension, Timothy E. Vanderleest, Celia M. Smits, Yi Xie, Cayla E. Jewett, J Todd Blankenship, Dinah Loerke

Biological Sciences: Faculty Scholarship

Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties of cell–cell interfaces, while the function of tricellular vertices has remained unaddressed. Here, we identify a highly novel mechanism in which vertices demonstrate independent sliding behaviors along cell peripheries to produce the topological deformations responsible for intercalation. Through systematic analysis, we find that the motion of vertices connected by contracting interfaces is not physically coupled, but instead possess strong radial coupling. E-cadherin and Myosin II exist in previously unstudied populations at cell …

Inventory Of The Genus Craterellus Persoon From Kigoma Region, Tanzania, Donatha D Tibuhwa

Tanzania Journal of Science

There is scanty knowledge on macro fungi diversity in Kigoma region. Bioprospecting survey was undertaken in Miombo woodlands, Kigoma region of Tanzania that has never been explored. The survey sought to conduct an inventory of Craterellus Persoon. Conventional taxonomic techniques were used during the survey to characterize the collected materials. Both macro and micro morphological features including basidiocarp size, colour, shape, spore size and print colour were examined. High diversity of reputed macrofungi was observed. This study presents a preliminary inventory of the genus Craterellus Persoon in the country. Three species were described; two namely Craterellus cornucopioides (Linnaeus) Persoon and …

The Influence Of Agricultural Activities On The Diversity Of Rodents In Kindoroko Forest Reserve And Surrounding Areas, North Pare Mountains, Tanzania, Remmy Lema, Flora J Magige

Tanzania Journal of Science

The patterns of richness, diversity and similarity in rodent communities in agricultural areas and in adjacent Kindoroko Forest Reserve in the North Pare Mountains, Tanzania were assessed. By using a capture-mark-recapture method, 289 individuals of nine rodent species in March and May 2011 were captured. Seven species were recorded in agricultural area and 5 species in the Forest Reserve. Multimammate Rat (Mastomys natalensis) was abundant in agricultural area while soft-furred mouse (Praomys delectorum) was dominant in the Forest Reserve. Species diversity was greater outside than inside the forest, supporting results of earlier studies. Species similarity between the two habitats was …

Distribution Patterns Of Gastrointestinal Parasites In Vervet Monkeys (Chlorocebus Pygerythrus) At Gombe National Park, Tanzania, Jared S. Bakuza

Tanzania Journal of Science

Vervet monkeys at Gombe National Park in western Tanzania constitute a key component of the park’s ecosystem through interactions with other animals and people in the area. However, the parasite fauna of these animals has not been investigated. Thus, 21 faecal samples obtained from the animals in September 2010 were examined for parasites using formol-ethyl technique. The parasites observed included Trichuris spp., Physaloptera spp., hookworms and unidentified nematodes. While egg counts for unidentified nematodes did not vary with vervet monkey communities (z = 0.759, p = 0.448), other parasites were significantly higher among vervets at north of the park near …

In Vitro Antimicrobial Activity Of Extracts Of Some Plant Species Used In The Management Of Hiv/Aids In Tanzania, Cyprian Beda Mpinda, Debra Meyer, Sylvester Leonard Lyantagaye

Tanzania Journal of Science

Six plants species (Harungana madagascariensis, Sapium ellipticum, Teclea nobilis, Pseudospondias microcarpa, Rauvolfia vomitoria and Psorospermum febrifugum) which are used in managing HIV/AIDS in Bukoba, Tanzania were screened for their antimicrobial effect on the selected representatives of infectious bacterial and fungal species. Gram-positive bacteria Streptococcus lactis, Gram-negative bacteria Klebsiella pneumoniae and a fungus Candida albicans were tested for their sensitivity to the treatment with selected plants extracts using disc diffusion and broth dilution techniques. Extracts of S. ellipticum, P. febrifegum and P. microcarpa demonstrated growth inhibition of S. lactis with the MIC ranging from 3.2 to 100 mg/ml. The extracts of …

Diet Composition Of The Golden Jackal (Canis Aureus) In The Ngorongoro Crater, Tanzania, Se Temu, Cuthbert L Nahonyo, Pd Moehlman

Tanzania Journal of Science

The Ngorongoro crater is known for having the highest density of carnivores in the world. In the past, most of the research focused on large carnivores such as lions and spotted hyenas. Medium sized carnivores such as jackals have received very little attention and the information on these carnivores including their diet composition is limited. Diet composition of golden jackal(Canis aureus) was studied in the Ngorongoro crater from July 2014 to May 2015 covering both dry and wet seasons using focal animal observation (direct method) and  collection (and analysis) of faecal/scat samples from known individuals in the field (indirect method). …

The Plant Species Composition, Diversity And Natural Regeneration Of Indigenous Trees In The Disturbed Ruvu South Forest Reserve, Tanzania, Cosmas Mligo

Tanzania Journal of Science

Ruvu Forest Reserve is among coastal forests in Tanzania that has been impacted by the increased anthropogenic activities. It was determined the plant species composition, diversity and indigenous trees regeneration growth size structure in the degraded Ruvu South Forest. Transect method was used to collect data that were treated using t-test. A total of 110 plant species distributed among 38 families were recorded such that Holarrhena pubescens, Hymenocardia ulmoides, Millettia micans, Ochna mossambicensis, Pseudolachnostylis maprouneifolia and Strychnos madagascariensis were the most common trees in moderately disturbed site, whereas Albizia versicolor, Pteleopsis myrtifolia, Pterocarpus angolensis, Antiaris toxicaria, Diplorhynchus condylocarpon and Terminalia …

Olfactory Learning Capabilities Of Paraphrynus Laevifrons, John Perez

Honors Program: Senior Projects (Public)

Amblypygids, a species of nocturnal arachnids from the tropics and subtropics are incredibly intelligent and are able to not only navigate through difficult tropical terrain but also, it is believed, possess olfactory learning capabilities that aid in navigation and recollection of environments. It is hypothesized that through sensitive olfactory receptors on their antenniform legs and highly developed mushroom bodies, Amblypygi can not only learn smells but also learn to associate smells with certain stimuli such as a crevice to take refuge in. To test this hypothesis, Paraphrynus laevifrons, a species of amplypygid was subjected to four different learning treatment groups …

Fatty Acid And Associated Gene Expression Analyses Of Three Tree Peony Species Reveal Key Genes For Α-Linolenic Acid Synthesis In Seeds, Qing-Yu Zhang, Rui Yu, Li-Hang Xie, Mahbubur Rahman, Aruna Kilaru, Li-Xin Niu, Yan-Long Zhang

ETSU Faculty Works

The increasing demand for healthy edible oil has generated the need to identify promising oil crops. Tree peony (Paeonia section Moutan DC.) is a woody oil crop with α-linolenic acid contributing for 45% of the total fatty acid (FA) content in seeds. Molecular and genetic differences that contribute to varied FA content and composition among the wild peony species are however, poorly understood. Analyses of FA content and composition during seed development in three tree peony species (P. rockii, P. potaninii, and P. lutea) showed varied FA content in the three species with highest in P. rockii, followed by P. …

Male Competition And Speciation: Expanding Our Framework For Speciation By Sexual Selection, Alycia C R Lackey, Michael D. Martin, R. M. Tinghitella

Biological Sciences: Faculty Scholarship

Sexual selection is a powerful source of rapid evolutionary change, and there is a long-standing hypothesis that it can cause reproductive isolation. However, our understanding of speciation by sexual selection is largely limited to mechanisms by which sexual selection via female mate choice can drive divergence (i.e., when male mating signals and female preferences for those signals diversify; Panhuis et al. 2001; Maan and Seehausen 2011). Male competition for mates—Darwin’s second mechanism of sexual selection—can also favor rapid and dramatic phenotypic and genotypic changes, yet it has been all but overlooked in speciation research (Darwin 1859, …

Differentially-Dimensioned Furrow Formation By Zygotic Gene Expression And The Mbt, Yi Xie, J Todd Blankenship

Biological Sciences: Faculty Scholarship

Despite extensive work on the mechanisms that generate plasma membrane furrows, understanding how cells are able to dynamically regulate furrow dimensions is an unresolved question. Here, we present an in-depth characterization of furrow behaviors and their regulation in vivo during early Drosophila morphogenesis. We show that the deepening in furrow dimensions with successive nuclear cycles is largely due to the introduction of a new, rapid ingression phase (Ingression II). Blocking the midblastula transition (MBT) by suppressing zygotic transcription through pharmacological or genetic means causes the absence of Ingression II, and consequently reduces furrow dimensions. The analysis of compound chromosomes that …

The Ecology Of Land Managers In Riparian Restoration, Lisa Buie Clark

Electronic Theses and Dissertations

While previous studies in restoration ecology have focused on the efficacy of direct management actions, the driving forces on management decisions (e.g., managers' characteristics or attitudes, environmental conditions) and the indirect impacts on restoration outcomes from management decisions (such as whether to collaborate) are quantified here for the first time. As a case study, I used data from 244 sites across the riparian Southwest US where the invasive shrubby tree Tamarix sp. was removed using various different methods. I surveyed and interviewed the 45 land managers who were responsible for the removal projects to determine their characteristics, attitudes, and …

Effect Of Habitat Fragmentation On Diversity And Abundance Of Nesting Birds In An Urban Landscape: The Case Of Mwalimu Nyerere Campus Thickets, University Of Dar Es Salaam, Tanzania, John Shirima, Chacha Werema

Tanzania Journal of Science

Little is known of the nesting bird species in most of urban areas of East Africa. An investigation was made of the occurrence of breeding bird species in the fragmented thickets found in the Mwalimu Nyerere Campus of the University of Dar es Salaam, in the City of Dar es Salaam. The study examined how habitat fragmentation affects nesting bird species richness and diversity in the thickets. Systematic physical nest searches of breeding birds were conducted in the fragmented thickets. Searches were conducted in transects and they included inspecting vegetation, bare ground, cavities, stream (river) banks and other suitable nesting …

Cytotoxicity, Antimicrobial And Antioxidant Activities Of Boletus Bicolor, A Basidiomycetes Mushroom Indigenous To Tanzania, Donatha Tibuhwa

Tanzania Journal of Science

Boletus bicolor commonly known as coloured bolete is a basidiomyctes mushroom species widely used as a delicacy and its extract has been used traditionally to treat several human ailments in Tanzania. This study evaluates its extracts in order to provide a scientific foundation on the possible application in folk medicine system based on the biochemical evaluation. Standard microbes Bacillus subtilis and Candida albicans were used to test, the antifungal and antibacterial activity.  Cytotoxicity test was also done on the extracts using brine shrimp test. Besides, the extracts were also used to investigate for antioxidant activity using DPPH  (1, 1-diphenyl-2-picrylhydrazyl radical). …

A Comparative Study Of Species Diversity Of Migrant Birds Between Protected And Unprotected Areas Of The Hadejia-Nguru Wetlands, Nigeria, As Ringim, Fj Magige, Rm Jasson

Tanzania Journal of Science

Among the most complex and fascinating behaviour in birds is their long, non-stop migration. Despite Hadejia-Nguru Wetlands (Ramsar site) being an important wintering ground for migratory birds, little is known about the diversity while density is completely lacking. This study assessed the status of migratory birds in the wetland’s Protected Areas (PAs) and Unprotected Areas (UPAs). A total of 99 census points spaced 400 m apart with radius of 100 m were surveyed from 14 wetlands (48 point count stations in the PAs and 51 in the UPAs). A total of 54 migrant bird species belonging to 13 orders and …

Effect Of Natural Blue-Green Algal Cells Lysis On Freshwater Quality, Kessy Kilulya, Titus Msagati, Bhekie Mamba

Tanzania Journal of Science

Blue-green algae grow in freshwater bodies when they are provided with suitable factors such as nutrients and appropriate weather conditions. Their cell lysis occurs naturally when they encounter unfavourable conditions.  This study reports on the types and amounts of fatty acids added into freshwater due to the algal cell lysis. The investigation on the amount of fatty acids released into water due to algal cells lysis was performed by placing samples in two compartments, one with no light allowing the algae cells to die while the other compartment was kept in favourable conditions enough to sustain the life of algae. …