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2023

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Articles 661 - 690 of 1823

Full-Text Articles in Biology

Exploring The Influence Of Online Inquiry Learning With E-Scaffolding Prompting Question On Problem-Solving Skills In The Work And Energy Topic For Islamic Senior High School Students, Arrika Wifqotu Lailin Nafisah, Supriyono Koes Handayanto, Sunaryono Sunaryono, Chokchai Yuenyong Jun 2023

Exploring The Influence Of Online Inquiry Learning With E-Scaffolding Prompting Question On Problem-Solving Skills In The Work And Energy Topic For Islamic Senior High School Students, Arrika Wifqotu Lailin Nafisah, Supriyono Koes Handayanto, Sunaryono Sunaryono, Chokchai Yuenyong

Jurnal Pendidikan Sains

This comprehensive investigation delves into the impact of integrating e-scaffolding prompting questions into online inquiry learning on students' problem-solving skills (PSS). With an explanatory design and a mixed-methods approach, the study focuses on XI-MIPA class students at MAN 1 Kediri City during the academic year 2022-2023. Utilizing meticulously crafted learning instruments, five problem-solving skills questions, and precise interview guidelines, the research employs triangulation through pre-tests, post-tests, and in- depth interviews. Quantitative data undergoes rigorous scrutiny via Analysis of Covariance (ANCOVA). The primary objective is to unveil the discernible influence of online inquiry learning complemented by e-scaffolding on the nuanced domain …

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Study Of Scientific Reasoning And Concept Mastery Of Students Through Guided Inquiry Learning Model Assisted By Formative Assessment, Selvina Visbad Saiya, Sentot Kusairi, Sunaryono Sunaryono Jun 2023

Study Of Scientific Reasoning And Concept Mastery Of Students Through Guided Inquiry Learning Model Assisted By Formative Assessment, Selvina Visbad Saiya, Sentot Kusairi, Sunaryono Sunaryono

Jurnal Pendidikan Sains

This study endeavors to assess the impact of guided inquiry learning, complemented by formative assessment, on students’ scientific reasoning (SR) and concept mastery (CM). The research involved 20 students from class XI A at SMA Muhammadiyah 02 Sumberpucung, utilizing questions adapted from the modified Lawson classroom test of scientific reasoning (MLCTSR) to measure SR, and the force concept inventory (FCI) to gauge CM. The instructional focus was Newton’s law of motion. Both qualitative and quantitative analyses were employed for data interpretation. The findings revealed a substantial influence of the guided inquiry learning model on SR, with a d-effect size value …

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The Effect Of Online Discovery-Inquiry Learning On Improving Critical Thinking Ability And Concept Mastery In Temperature And Heat Materials, Fildzah Purvita Dewi, Lia Yuliati, Edi Supriana Jun 2023

The Effect Of Online Discovery-Inquiry Learning On Improving Critical Thinking Ability And Concept Mastery In Temperature And Heat Materials, Fildzah Purvita Dewi, Lia Yuliati, Edi Supriana

Jurnal Pendidikan Sains

The research endeavors to investigate the impact of implementing discovery-inquiry learning concerning the topic of temperature and heat on the enhancement of students’ critical thinking abilities and concept mastery. The study adopted a quasi-experimental design, employing a pretest-posttest control group framework. A cluster random sampling technique was applied to select the research sample, which comprised 36 students from class XI MIPA 3 (control group) and class XI MIPA 4 (experimental group) at SMA Negeri Rambipuji. The assessment instruments employed were two essay-shaped tests, specifically designed for evaluating critical thinking ability and concept mastery. Data analysis involved the utilization of independent …

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Utilization Of Palm Waste Charcoal Briquettes (Abls) As Thematic-Based Integrated Science Learning Materials For Middle School Students, Sausan Fatin Nurazizah, Rendy Wikrama Wardana, Bhakti Karyadi, Henny Johan, Deni Parlindungan, Fitri Mulyani, Lasriani Lasriani Jun 2023

Utilization Of Palm Waste Charcoal Briquettes (Abls) As Thematic-Based Integrated Science Learning Materials For Middle School Students, Sausan Fatin Nurazizah, Rendy Wikrama Wardana, Bhakti Karyadi, Henny Johan, Deni Parlindungan, Fitri Mulyani, Lasriani Lasriani

Jurnal Pendidikan Sains

This investigation was conducted with the objective of evaluating the efficacy of ABLS ignition and formulating instructional materials for junior high school science education through the integration of e-booklets containing thematic-based science content. The research design employed herein adheres to the principles of experimentation and model development, falling under the category of Research and Development (R&D). The experimental cohort was stratified into three groups denoted as A1 (70% shell charcoal: 30% bunch charcoal), A2 (50% shell charcoal: 50% bunch charcoal), and A3 (30% shell charcoal: 70% bunch charcoal). The development of science learning materials was realized through integration with cobwebs, …

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The Presence Of Unusual Foramina In The Femoral Intercondylar And Trochanteric Fossae On Skeletonized Individuals From Tanzania, Wilson Jilala, David Ngassapa, Ali Hamad Jun 2023

The Presence Of Unusual Foramina In The Femoral Intercondylar And Trochanteric Fossae On Skeletonized Individuals From Tanzania, Wilson Jilala, David Ngassapa, Ali Hamad

Tanzania Journal of Science

According to the standard nomenclature, skeletal land marks are all identified by their given names in the world of human skeletal anatomy. But it is also clear that the discovery of new anatomical features continues because human beings have so many anatomical variations caused by genetics, functions, geographical adaptations, and diseases; they are all described and given names. Therefore, an anatomical feature that is rare and undescribed is certainly a discovery and it deserves to be recorded. The discovery of anatomical features on the epiphyseial ends of the femur reported here adds two new names, namely the intercondylar fossa foramen …

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Prevalence And Burden Of Gastrointestinal Parasites Among University Students At Dar Es Salaam University College Of Education, Tanzania, Magreth Fulgence, Ummul-Khair Mustafa, Jared S. Bakuza Jun 2023

Prevalence And Burden Of Gastrointestinal Parasites Among University Students At Dar Es Salaam University College Of Education, Tanzania, Magreth Fulgence, Ummul-Khair Mustafa, Jared S. Bakuza

Tanzania Journal of Science

Gastrointestinal parasites cause major health problems in many tropical and sub-tropical countries including Tanzania. However, information on the status of these infections is often scanty, especially among young adults including students in higher learning institutions.  During December 2020 to June 2021, a total of 272 faecal samples from university students at Dar es Salaam University College of Education (DUCE) were examined for parasites using the Kato Katz technique and analysed for infection levels based on standard guidelines. Detected parasites were identified as Schistosoma mansoni (8.1%), hookworms (1.5%), Trichuris trichiura (0.4%) and Hymenolepis diminuta (0.4%), with an overall prevalence of 10.4%. …

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Feeding Habits And Diet Composition Of The African Sacred Ibis In Selected Water-Logged Sites In Dar Es Salaam, Tanzania, Jared S. Bakuza, Joyce Ndinadyo Jun 2023

Feeding Habits And Diet Composition Of The African Sacred Ibis In Selected Water-Logged Sites In Dar Es Salaam, Tanzania, Jared S. Bakuza, Joyce Ndinadyo

Tanzania Journal of Science

The African Sacred Ibises Threskiornis aethiopicus are generalist birds that feed on many invertebrates and some vertebrates. However, the diet composition of local T. aethiopicus populations in wetland areas in Dar es Salaam Tanzania was unknown. Sacred Ibises from eight water-logged areas in Dar es Salaam City, Tanzania were studied to determine their feeding habits and diet composition using direct observations and molecular analysis of faecal samples. Snails species identified from sites frequented by T. aethiopicus included Lymnaea natalensis (61.62%), Melanoides tuberculatus (20.64%), Bulinus sp. (17.66%) and Anisus sp. (0.08%), while molecular analysis of the birds’ diet revealed presence of …

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Efficacy Of The Botanical Extracts, Azadirachta Indica (Sapindales: Meliaceae) And Tagetes Minuta (Asterales: Asteraceae) In The Control Of Cabbage Insect Pests In Iringa District, Tanzania, Levocatus M. Rwegoshora, Vendeline E. Tairo, Moses I. Olotu Jun 2023

Efficacy Of The Botanical Extracts, Azadirachta Indica (Sapindales: Meliaceae) And Tagetes Minuta (Asterales: Asteraceae) In The Control Of Cabbage Insect Pests In Iringa District, Tanzania, Levocatus M. Rwegoshora, Vendeline E. Tairo, Moses I. Olotu

Tanzania Journal of Science

Although botanical extracts have been widely studied globally, the efficacy of neem and Mexican marigold against cabbage insect pests is scanty. Field experiments were conducted at Kalenga and Mgera in 2022 using a randomized complete block design. Four treatments (neem, Mexican marigold, a mixture of the two extracts and untreated) were replicated three times. In Kalenga, damage levels for treated plots varied significantly from 3.33 to 9.20% and 5.0 to 18.33%, while for untreated plots varied from 37.70 to 45.85% during the rainy and dry seasons, respectively (F(3, 499) = 111.71, p < 0.05). A similar trend was recorded in Mgera, the damage levels varied significantly between treated (4.44–15.83%) and untreated plots (34.40–46.60%) (F(3, 449) = 94.23, p < 0.05). Treated plots had higher marketable cabbage yields (30.6–43.10 t/ha) than untreated plots (4.78–11.20 t/ha), which differed significantly in Kalenga (F (3, 67) = 141.79, p < 0.05) and Mgera (F (3, 67) = 53.36, p < 0.05). These extracts have shown insecticidal properties, can serve as promising candidates for further studies aimed at isolating active compounds for scaling up ecologically friendly strategies of controlling pests and improve the quality of cabbage products.

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Molecular Epidemiology Of Antibiotic Resistance Among Escherichia Coli Isolated From Broiler Chickens Sold At Selected Markets In Dar Es Salaam, Tanzania, Abdilahi H. Kiula, Victor A. Makene Jun 2023

Molecular Epidemiology Of Antibiotic Resistance Among Escherichia Coli Isolated From Broiler Chickens Sold At Selected Markets In Dar Es Salaam, Tanzania, Abdilahi H. Kiula, Victor A. Makene

Tanzania Journal of Science

Unwarranted and improper uses of antibiotics in broiler farms contribute to the challenge of antibiotic resistance, making even previously treatable infections, difficult to treat. We conducted a cross sectional study from November 2021 to May 2022 from broiler chicken markets in four districts of Dar es Salaam to determine the extent of antibiotic resistance among E. coli isolates from broiler chickens. A total of 160 E. coli isolates recovered from cloacal swabs were identified by culture and biochemical tests and confirmed by Polymerase Chain Reaction (PCR) assays. Antimicrobial susceptibility testing, targeting seven classes of antibiotics, was performed by disk diffusion …

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Application Of Agile And Simulation Approaches For The Maximal Benefits Of Reduced Turnaround Time From The Point Of Care Testing, Yusta W. Simwita, Berit I. Helgheim Jun 2023

Application Of Agile And Simulation Approaches For The Maximal Benefits Of Reduced Turnaround Time From The Point Of Care Testing, Yusta W. Simwita, Berit I. Helgheim

Tanzania Journal of Science

This paper uses simulation to explore improvement opportunities in the orthopaedic care process. Secondly, this study used a combination of simulation and agile strategies to explore agile operational plans that can be adopted  to maximize the benefits of reduced turnaround time. Data used for this study was collected by observing the entire orthopaedic care process. A total of 635 observations were obtained, with 179 out of these observations undergoing the whole care process. The actual collected patient data were compared with the simulation output to validate the developed simulation. Different scenarios were developed to test operational plans for the maximal …

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Ethnobotanical Survey, Consumption Pattern And Genetic Conservation Of Leafy Vegetables In South-West Nigeria, Temitope O Onuminya, Michael I Osundinakin, Oluwatoyin T Ogundipe Jun 2023

Ethnobotanical Survey, Consumption Pattern And Genetic Conservation Of Leafy Vegetables In South-West Nigeria, Temitope O Onuminya, Michael I Osundinakin, Oluwatoyin T Ogundipe

Tanzania Journal of Science

Leafy vegetables are important in human nutrition; however, their diversity as seen in the dishes in South western Nigeria appears to be diminishing. Many popular delicacies are no longer available in the markets. Therefore, this research was conducted to identify and document leafy vegetables in South West Nigeria, assess their ethnobotanical uses from questionnaires administered to farmers/sellers, secure their genetic resource base, and improve their value chain in South-West Nigeria. Vegetable samples were collected from different local communities in five South-West states in Nigeria, identified using manual/flora, and authenticated at the University of Lagos Herbarium. One thousand respondents, 200 in …

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Genetic Analysis Reveals Substantial Proportion Of Non-Targeted Tilapias Among Farmed Stocks In Kilosa And Kibaha, Tanzania, Winifrida L Mbilinyi, Cyrus Rumisha, Augustine W Mwandya, George M Msalya Jun 2023

Genetic Analysis Reveals Substantial Proportion Of Non-Targeted Tilapias Among Farmed Stocks In Kilosa And Kibaha, Tanzania, Winifrida L Mbilinyi, Cyrus Rumisha, Augustine W Mwandya, George M Msalya

Tanzania Journal of Science

Nile tilapia farmers in Tanzania have been complaining about slow growth and low yields. Since some farmers obtain their seeds from unreliable sources, they may potentially be dealing with various non-targeted tilapias that require different environments and treatments to enhance growth and yields. Thus, this study analysed fragments (600 base pairs) of the cytochrome oxidase subunit I gene (COI) from 74 farmed tilapias in Kilosa and Kibaha, Tanzania to determine the presence of non-targeted tilapias. An additional 42 samples from the Magadu Fish Farm, Mindu dam, and Lake Victoria were included for comparison. The findings revealed that non-targeted tilapias accounted …

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Ameliorative Effects Of Vitamins C And E On Haematotoxicity And Spleen Histopathology Induced By Dichlorvos Insecticide In Female Wistar Rats, Halima Mfaume, Harishchandra Pratap, Claudius Luziga, Felister Urasa Jun 2023

Ameliorative Effects Of Vitamins C And E On Haematotoxicity And Spleen Histopathology Induced By Dichlorvos Insecticide In Female Wistar Rats, Halima Mfaume, Harishchandra Pratap, Claudius Luziga, Felister Urasa

Tanzania Journal of Science

This research aimed to assess the toxicological effects of dichlorvos insecticide (2,2-dichlorovinyl dimethyl phosphate or DDVP) on haematological indices of female Wistar rats and to investigate the antioxidant properties of vitamins C and E in ameliorating haematotoxicity. The rats were divided into 6 groups; control-water, control-oil, dichlorvos group, dichlorvos + vitamin C group, dichlorvos + vitamin E group and dichlorvos + vitamin C + E group. Dichlorvos and vitamins were orally administered to rats with an interval of half an hour between treatments. The control-oil group was given 2 ml corn oil and the control-water group was given water adlib. …

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Dna Barcoding Reveals Occurrence Of Cardiocephaloides Sp. (Digenea: Strigeidae) Infecting The Great Cormorant Phalacrocorax Carbo (L. 1758) In Mwanza Gulf, Lake Victoria, Fred D Chibwana Jun 2023

Dna Barcoding Reveals Occurrence Of Cardiocephaloides Sp. (Digenea: Strigeidae) Infecting The Great Cormorant Phalacrocorax Carbo (L. 1758) In Mwanza Gulf, Lake Victoria, Fred D Chibwana

Tanzania Journal of Science

Cormorants (Pelecaniformes) are widely distributed worldwide, occurring as coastal birds in inland waters and marine environments, and have been implicated in the transmission of some aquatic parasites. As such, the objective of the present work was to investigate the occurrence and morphological variations in Cardiocephaloides (Digenea: Strigeidae), parasites of the great cormorant Phalacrocorax carbo. The Cardiocephaloides specimens used for molecular analysis (DNA barcoding (cox1) region) were obtained from the intestines of the great cormorants collected from Mwanza Gulf in Lake Victoria. Morphological examination of Cardiocephaloides specimens showed the possible co-existence of four morphospecies belonging to the genus Cardiocephaloides. However, detailed …

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Determination Of Epigallocatechin Gallate (Egcg) And Antibacterial Activities Of Commercially Available Tanzanian Green Tea (Camellia Sinensis), Raphael J Shedafa, Joseph Sempombe, Ramadhan So Nondo, Eliangiringa Kaale, Mary Temu, Peter Imming Jun 2023

Determination Of Epigallocatechin Gallate (Egcg) And Antibacterial Activities Of Commercially Available Tanzanian Green Tea (Camellia Sinensis), Raphael J Shedafa, Joseph Sempombe, Ramadhan So Nondo, Eliangiringa Kaale, Mary Temu, Peter Imming

Tanzania Journal of Science

This paper presents the results of the antibacterial activity of green tea extract (Camellia sinensis) against Staphylococcus aureus (ATCC 29213), Escherichia coli (ATCC 25922), and Klebsiella pneumoniae (ATCC 708903). Quantitative and qualitative measurements of epigallocatechin gallate (EGCG) have also been reported. The analysis by High Performance Thin Layer Chromatography (HPTLC) revealed the presence of epigallocatechin gallate (EGCG) in the green tea extracts ranging from 13.16 to 18.64 mg/g. Crude extracts GT 01–GT 03 inhibited a greater number of microorganisms and presented the lowest values of MIC against Staphylococcus aureus (0.625 mg/mL), Escherichia coli (0.625 mg/mL), and Klebsiella pneumoniae (1.25 mg/mL). …

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In-Vitro Antisickling Activity Of Pergularia Daemia, Canna Indica And Petiveria Alliacea Plants Used In The Treatment Of Sickle Cell Anaemia In Edo State, Nigeria, Osaro Iyekowa, Felix E Okieimen, Clifford O Ehisuoria Jun 2023

In-Vitro Antisickling Activity Of Pergularia Daemia, Canna Indica And Petiveria Alliacea Plants Used In The Treatment Of Sickle Cell Anaemia In Edo State, Nigeria, Osaro Iyekowa, Felix E Okieimen, Clifford O Ehisuoria

Tanzania Journal of Science

Sickle cell disease is a genetic blood disorder that affects the shape and transportation of the red blood cells (RBCs) in blood vessels, leading to various clinical complications. Available medicines for treating the disease are insufficiently effective, toxic or expensive. Therefore, there has been a pressing need for effective and inexpensive therapeutic agents from indigenous medicinal plants. Petiveria alliacea, Canna indica and Pergularia daemia, respectively were evaluated for their cationic constituents as a measure for their antisickling activity in sickle cell anaemia disorder. The three medicinal plants were extracted separately with methanol solvent using maceration method for 72 hours. The …

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Synthesis, Characterization And Antimicrobial Properties Of Mixed-Ligand Complexes Of Some Metal(Ii) Ions With Barbituric Acid And 1,10-Phenanthroline Ligands, Kayode Taiwo Ishola, Oluwabiyi James Olaoye, Mary Adelaide Oladipo, Omobola Ajibike Odedokun, Oyebanji Oluseun Aboyeji Jun 2023

Synthesis, Characterization And Antimicrobial Properties Of Mixed-Ligand Complexes Of Some Metal(Ii) Ions With Barbituric Acid And 1,10-Phenanthroline Ligands, Kayode Taiwo Ishola, Oluwabiyi James Olaoye, Mary Adelaide Oladipo, Omobola Ajibike Odedokun, Oyebanji Oluseun Aboyeji

Tanzania Journal of Science

Many pathogenic organisms have developed resistance to many antibiotics, which leads to an increase in the spread of many microbial infections. There is an urgent need to find more effective drugs to curb widespread transmission. Hence, this study synthesized and evaluated the antimicrobial activities of mixed-ligand complexes of Cu(II), Co(II), Ni(II), Mn(II), and Zn(II) ions with barbituric acid and 1,10 phenanthroline ligands in effort to find more effective antibiotics. The complexes were characterized using elemental analysis, metal analysis, melting points, solubility tests, and spectroscopic analyses (IR and UV-visible). The antimicrobial activities of the complexes were evaluated against two gram-positive bacteria …

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Shower Thoughts: Why Scientists Should Spend More Time In The Rain, John T. Van Stan Ii, Scott T. Allen, Douglas P. Aubrey, Z. Carter Berry, Matthew Biddick, Miriam A.M.J. Coenders-Gerrits, Paolo Giordani, Sybil G. Gotsch, Ethan D. Gutmann, Yakov Kuzyakov, Donát Magyar, Valentina S.A. Mella, Kevin E. Mueller, Alexandra G. Ponette-González, Philipp Porada, Carla E. Rosenfeld, Jack Simmons Jun 2023

Shower Thoughts: Why Scientists Should Spend More Time In The Rain, John T. Van Stan Ii, Scott T. Allen, Douglas P. Aubrey, Z. Carter Berry, Matthew Biddick, Miriam A.M.J. Coenders-Gerrits, Paolo Giordani, Sybil G. Gotsch, Ethan D. Gutmann, Yakov Kuzyakov, Donát Magyar, Valentina S.A. Mella, Kevin E. Mueller, Alexandra G. Ponette-González, Philipp Porada, Carla E. Rosenfeld, Jack Simmons

Biological, Geological, and Environmental Faculty Publications

Stormwater is a vital resource and dynamic driver of terrestrial ecosystem processes. However, processes controlling interactions during and shortly after storms are often poorly seen and poorly sensed when direct observations are substituted with technological ones. We discuss how human observations complement technological ones and the benefits of scientists spending more time in the storm. Human observation can reveal ephemeral storm-related phenomena such as biogeochemical hot moments, organismal responses, and sedimentary processes that can then be explored in greater resolution using sensors and virtual experiments. Storm-related phenomena trigger lasting, oversized impacts on hydrologic and biogeochemical processes, organismal traits or functions, …

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Influence Of Woody Vegetation Composition And Structure On Fuels And Prescribed Fire In Mountain Longleaf Restoration, Collin J. Anderson Jun 2023

Influence Of Woody Vegetation Composition And Structure On Fuels And Prescribed Fire In Mountain Longleaf Restoration, Collin J. Anderson

Master of Science in Integrative Biology Theses

Longleaf pine (LLP) ecosystems have experienced a widespread ecological state shift largely due to fire exclusion which has allowed mesophytes, i.e., shade-tolerant, often fire-sensitive species to encroach, reducing flammability and biodiversity through a process known as “mesophication.” Although prescribed fire is commonly used to reverse mesophication, fire behavior, and thus prescribed fire utility for this purpose, is poorly characterized in mixed pine-hardwood stands with mesophyte encroachment. This study aimed to identify mechanisms by which tree composition, structure, and fuels contribute to fire behavior, focusing on the understudied mountain longleaf pine (MLLP) ecoregion in northwest Georgia. I hypothesized that woody vegetation …

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Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang Jun 2023

Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang

Michigan Tech Publications, Part 1

MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression by targeting specific messenger RNAs (mRNAs) in distinct cell types. This review provides a com-prehensive overview of the current understanding regarding the involvement of miR-483-5p and miR-483-3p in various physiological and pathological processes. Downregulation of miR-483-5p has been linked to numerous diseases, including type 2 diabetes, fatty liver disease, diabetic nephropathy, and neurological injury. Accumulating evidence indicates that miR-483-5p plays a crucial protective role in preserving cell function and viability by targeting specific transcripts. Notably, elevated levels of miR-483-5p in the bloodstream strongly correlate with metabolic risk factors and …

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Development Of A 16s Reference Library For Edna Metabarcoding The Freshwater Fishes Of Western Ecuador., Hannah M. Willis, Olivia G. Schweikart, Windsor E. Aguirre Jun 2023

Development Of A 16s Reference Library For Edna Metabarcoding The Freshwater Fishes Of Western Ecuador., Hannah M. Willis, Olivia G. Schweikart, Windsor E. Aguirre

DePaul Discoveries

This project examines the use of the 16S locus to amplify neotropical freshwater fishes native to Western Ecuador in a newly created 16S reference library for DNA barcoding and eDNA metabarcoding applications. Among the orders Characiformes, Siluriformes, Cichliformes, Gobiiformes, Cryprinodontiformes, Gymnotiformes, and Perciformes, a compendium of 105 specimens were sequenced, with 43 representing new 16S sequences previously unavailable on Genbank.

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An Assessment Of The Impact Of Raman Based Glucose Feedback Control On Cho Cell Bioreactor Process Development, Luke Gibbons, Francis Maslanka, Nikky Le, Al Magill, Pankaj Singh, Joseph Mclaughlin, Fiona Madden, Ronan Hayes, Barry Mccarthy, Christopher Rode, Jim O'Mahony, Rosemary Rea, Caitlin O'Mahony-Hartnett Jun 2023

An Assessment Of The Impact Of Raman Based Glucose Feedback Control On Cho Cell Bioreactor Process Development, Luke Gibbons, Francis Maslanka, Nikky Le, Al Magill, Pankaj Singh, Joseph Mclaughlin, Fiona Madden, Ronan Hayes, Barry Mccarthy, Christopher Rode, Jim O'Mahony, Rosemary Rea, Caitlin O'Mahony-Hartnett

Department of Biological Sciences Publications

Process analytical technology (PAT) tools such as Raman Spectroscopy have become established tools for real time measurement of CHO cell bioreactor process variables and are aligned with the QbD approach to manufacturing. These tools can have a significant impact on process development if adopted early, creating an end-to-end PAT/QbD focused process. This study assessed the impact of Raman based feedback control on early and late phase development bioreactors by using a Raman based PLS model and PAT management system to control glucose in two CHO cell line bioreactor processes. The impact was then compared to bioreactor processes which used manual …

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Comparative Toxicity Assessment Of Glyphosate And Two Commercial Formulations In The Planarian Dugesia Japonica, S. Grace Fuselier , '25, Danielle Ireland, Nicholas Fu , '25, Christina Rabeler, Eva-Maria S. Collins Jun 2023

Comparative Toxicity Assessment Of Glyphosate And Two Commercial Formulations In The Planarian Dugesia Japonica, S. Grace Fuselier , '25, Danielle Ireland, Nicholas Fu , '25, Christina Rabeler, Eva-Maria S. Collins

Biology Faculty Works

Introduction: Glyphosate is a widely used, non-selective herbicide. Glyphosate and glyphosate-based herbicides (GBHs) are considered safe for non-target organisms and environmentally benign at currently allowed environmental exposure levels. However, their increased use in recent years has triggered questions about possible adverse outcomes due to low dose chronic exposure in animals and humans. While the toxicity of GBHs has primarily been attributed to glyphosate, other largely unstudied components of GBHs may be inherently toxic or could act synergistically with glyphosate. Thus, comparative studies of glyphosate and GBHs are needed to parse out their respective toxicity. Methods: We performed such a comparative …

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Resource Recovery From Crew Metabolic Wastes In Lunar And Martian Habitats: Reuse Of Effluent From An Anaerobic Membrane Bioreactor Treating Fecal Organic Matter As Fertilizer Source For Plants, Alexandra A. Smith Jun 2023

Resource Recovery From Crew Metabolic Wastes In Lunar And Martian Habitats: Reuse Of Effluent From An Anaerobic Membrane Bioreactor Treating Fecal Organic Matter As Fertilizer Source For Plants, Alexandra A. Smith

USF Tampa Graduate Theses and Dissertations

As humans set their sights on exploration of the Moon and Mars human focused challenges like providing potable water, supplying nutritious food to the astronaut crew, and managing their waste present themselves. There are no readily available resources on the Moon and Mars, therefore nothing should be considered unusable including the organic wastes (i.e., feces and urine) produced by the crew. Fecal matter is not currently recycled and is treated as solid waste. After collection, it is discarded out of the International Space Station where it is incinerated upon re-entry. This approach will not work on the Moon’s surface. There …

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Variation In Salamander Life History And Community Composition Across An Urban Gradient In Atlanta, Georgia, Usa, Leah Rittenburg Jun 2023

Variation In Salamander Life History And Community Composition Across An Urban Gradient In Atlanta, Georgia, Usa, Leah Rittenburg

Master of Science in Integrative Biology Theses

Urbanization threatens to alter stream systems in watersheds in the eastern United States. These highly disturbed systems may result in many local extirpations of sensitive salamander species. One group of organisms that often persists in urban streams is the Eurycea bislineata (Two-lined Salamander) species complex. Many aspects of Two-lined Salamander life history are linked to environmental factors, particularly stream temperature, prey abundance, and stream hydrology. Urbanization threatens to alter these environmental factors, potentially influencing Two-lined Salamander life history. At 14 sites spanning an urban gradient in Atlanta, GA, I investigated the variation in life history and phenology in the Southern …

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Westward Expansion Of The Evening Bat (Nycticeius Humeralis) Into Dawson And Lincoln Counties Of Central Nebraska, Jamila Bajelan, Keith Geluso Jun 2023

Westward Expansion Of The Evening Bat (Nycticeius Humeralis) Into Dawson And Lincoln Counties Of Central Nebraska, Jamila Bajelan, Keith Geluso

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The Evening Bat (Nycticeius humeralis) is a small, migratory species of temperate woodlands in the eastern United States. Along its western distributional edge in the Great Plains, this species resides in wooded riverine corridors. In recent decades, the distribution of Evening Bats has expanded westward throughout the Great Plains. Herein, we report on two county records that further document the distributional expansion of Evening Bats into central Nebraska. In 2019 and 2022, Evening Bats were collected in Lincoln and Dawson counties, respectively, in woodlands adjacent to the Platte River. Increases in wooded habitats along prairie waterways likely has …

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Plains To Peaks: An Analysis Of The Relationship Between Colorado Butterfly Communities And Altitude, Anna O. James, Kathryn Hokamp, Chris García-Hellmuth, Richard P. Reading Jun 2023

Plains To Peaks: An Analysis Of The Relationship Between Colorado Butterfly Communities And Altitude, Anna O. James, Kathryn Hokamp, Chris García-Hellmuth, Richard P. Reading

Western North American Naturalist

We compared butterfly communities of the Plains, Foothills, and Montane Zones of the Colorado Front Range and examined 3 groups of butterflies—blues (Polyommatinae species), fritillaries (Speyeria and Boloria spp.), and painted ladies (species Vanessa cardui)—for altitude shifts between 2014 and 2020. We conducted these analyses using data collected by volunteer butterfly monitors from the Colorado Butterfly Monitoring Network, a group organized and run by Butterfly Pavilion in Westminster, Colorado. Monitors walked designated routes and counted all butterflies they saw, identifying each to the lowest possible taxonomic group they could. We sorted routes into the 3 altitude zones, organized …

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First Shasta Ground Sloth (Nothrotheriops; Xenarthra) From The Eastern Great Basin, Nevada, Jim I. Mead, Sandra L. Swift, H. Gregory Mcdonald, Steven D. Emslie Jun 2023

First Shasta Ground Sloth (Nothrotheriops; Xenarthra) From The Eastern Great Basin, Nevada, Jim I. Mead, Sandra L. Swift, H. Gregory Mcdonald, Steven D. Emslie

Western North American Naturalist

We present the first record of the Shasta ground sloth (Nothrotheriops shastensis) for the eastern Great Basin. The single right upper second molariform was recovered from the back dirt pile of archaeological test pits excavated during the 1960s and 1970s in Smith Creek Cave, northern Snake Range, White Pine County, Nevada. The precise age of the specimen is indeterminate, although all sediment layers in the area produced radiocarbon dates older than 11,000 cal BP; thus, the specimen is considered of the Rancholabrean Land Mammal Age. All previous accounts of this sloth in the Great Basin are from its …

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Leveraging Protein Dynamics To Drug Filovirus Protein-Nucleic Acid Interactions Using Simulations And Experiments, Matthew Alberto Cruz Jun 2023

Leveraging Protein Dynamics To Drug Filovirus Protein-Nucleic Acid Interactions Using Simulations And Experiments, Matthew Alberto Cruz

Arts & Sciences Graduate Student Theses and Dissertations

Drug discovery targeting protein nucleic acid (PNI) and protein-protein interactions (PPI) remains a difficult task. How to identify druggable conformations and discover compounds binding to these conformations is an unsolved problem. Here, I describe how we apply computational and experimental approaches to probe the conformational landscape of a key immune antagonist protein from the Zaire ebolavirus. Viral Protein 35 (VP35) binds to the viral pathogen associated molecular pattern, double stranded RNA (dsRNA), and blocks activation of the interferon (IFN) response. Long time scale molecular dynamics simulations reveal that VP35 adopts an alternative conformation which opens a pocket absent in experimental …

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Gabaergic Synaptic Scaling Is Triggered By Changes In Spiking Activity Rather Than Transmitter Receptor Activation, Carlos Gonzalez-Islas, Zahraa Sabra, Ming-Fai Fong, Pernille Bülow, Nicholas Au Yong, Kathrin Engisch, Peter Wenner Jun 2023

Gabaergic Synaptic Scaling Is Triggered By Changes In Spiking Activity Rather Than Transmitter Receptor Activation, Carlos Gonzalez-Islas, Zahraa Sabra, Ming-Fai Fong, Pernille Bülow, Nicholas Au Yong, Kathrin Engisch, Peter Wenner

Biological Sciences Faculty Publications

Homeostatic plasticity represents a set of mechanisms that are thought to recover some aspect of neural function. One such mechanism called AMPAergic scaling was thought to be a likely candidate to homeostatically control spiking activity. However, recent findings have forced us to reconsider this idea as several studies suggest AMPAergic scaling is not directly triggered by changes in spiking. Moreover, studies examining homeostatic perturbations in vivo have suggested that GABAergic synapses may be more critical in terms of spiking homeostasis. Here we show results that GABAergic scaling can act to homeostatically control spiking levels. We find that increased or decreased …

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