Biology Commons^™

Periodic Spatial Disturbances Alter The Expression Of Quorum Sensing Virulence Factors In Pseudomonas Aeruginosa, Laura García-Diéguez

All HCAS Student Capstones, Theses, and Dissertations

Pseudomonas aeruginosa is an opportunistic pathogen associated with severe acute and chronic illnesses. Current antibiotic-based approaches fail to effectively treat P. aeruginosa infections due to the effectiveness and robustness of the quorum sensing signaling system (QS). Pathogenic bacteria, such as P. aeruginosa, employ this population density-dependent communication mechanism to confer antimicrobial resistance, propagate infection, and coordinate the expression of virulence factors, through the production and detection of autoinducing signaling molecules (AI). As such, there is a growing interest in developing novel non-antibiotic-based techniques to attenuate the pathogenicity of P. aeruginosa by disrupting the functionality of its QS system. Previous …

Genetic Covariance In Immune Measures And Pathogen Resistance In Decorated Crickets Is Sex And Pathogen Specific, Corinne Letendre, Kristin Duffield, Ben M. Sadd, Scott K. Sakaluk, Clarissa M. House, John Hunt

Faculty Publications – Biological Sciences

1. Insects are important models for studying immunity in an ecological and evolutionary context. Yet, most empirical work on the insect immune system has come from phenotypic studies meaning we have a limited understanding of the genetic architecture of immune function in the sexes.
2. We use nine highly inbred lines to thoroughly examine the genetic relationships between a suite of commonly used immune assays (haemocyte count, implant encapsulation, total phenoloxidase activity, antibacterial zone of inhibition and pathogen clearance) and resistance to infection by three generalist insect pathogens (the gram-negative bacterium Serratia marcescens, the gram-positive bacterium Bacillus cereus and the fungus …

Is The Zebrafish Speed Bump Mutant A Mutation In Wee1? And Is It A Complete Loss Of Function?, Nathania Karyadi

Honors Theses

Every cell has to undergo the cell cycle process before it can divide or differentiate. The cell cycle is an error-prone complex process where mutations could happen. This study aimed to characterize the nervous system and blood cells of the speed bump mutant in zebrafish. This study also aimed to do gene characterization of the wee1 CRISPR mutant. The speed bump mutant is due to a mutation in the wee1 gene. Lack of the wee1 gene will cause no Wee1 Kinase, which inhibits the MPF, causing cells to go into mitosis too early. To fulfill these objectives, this study used …

The Various Nervous System Drugs Commonly Used For Treatment And The Disparities In Availability Of These Drugs In Minority Groups, Iman Haurami

Honors Scholars Collaborative Projects

The nervous system is a complex network in our body that is responsible for actions, reflexes, and even sensations. With the brain being part of the CNS, many common nervous system disorders are due to a defect in the central nervous system and the functions of this organ. These disorders include mental health problems, degenerative diseases, seizures, infections, and other possible genetic diseases. Many of these diseases are common in society and constantly researched. Even though these diseases are common, treatment accessibility and effects on people of minority groups are overlooked compared to the focus on majority groups. For drug …

A Role For Myosin Ii Clusters And Membrane Energy In Cortex Rupture For Dictyostelium Discoideum, Emmanuel Asante-Asamani, Daniel Grange, Devarshi Rawal, Zully Santiago, John Loustau, Derrick Brazill

Publications and Research

Blebs, pressure driven protrusions of the cell membrane, facilitate the movement of eukaryotic cells such as the soil amoeba Dictyostelium discoideum , white blood cells and cancer cells. Blebs initiate when the cell membrane separates from the underlying cortex. A local rupture of the cortex, has been suggested as a mechanism by which blebs are initiated. However, much clarity is still needed about how cells inherently regulate rupture of the cor- tex in locations where blebs are expected to form. In this work, we examine the role of mem- brane energy and the motor protein myosin II (myosin) in facilitating …

Exploring The Relationship Between Art And Environmental Education, Mackenzie Haynes

Honors Projects

To explore the relationship between art and environmental education, I created a lesson plan and then put into practice at Crim Elementary School in a 4th grade art class. The art project had to do with the environment, endangered animals and recyclable materials. I titled the project "Habitat Heroes" and students had to imagine that they were the only people left on Earth along with a few animals and lots of trash. They were tasked with selecting an endangered animal in Ohio from one of five different groups (mammals, birds, reptiles/amphibians, birds and fish) to create a habitat for. The …

Mechanobiology In The Comorbidities Of Ehlers Danlos Syndrome, Shaina P. Royer, Sangyoon J. Han

Michigan Tech Publications, Part 1

Ehlers-Danlos Syndromes (EDSs) are a group of connective tissue disorders, characterized by skin stretchability, joint hypermobility and instability. Mechanically, various tissues from EDS patients exhibit lowered elastic modulus and lowered ultimate strength. This change in mechanics has been associated with EDS symptoms. However, recent evidence points toward a possibility that the comorbidities of EDS could be also associated with reduced tissue stiffness. In this review, we focus on mast cell activation syndrome and impaired wound healing, comorbidities associated with the classical type (cEDS) and the hypermobile type (hEDS), respectively, and discuss potential mechanobiological pathways involved in the comorbidities.

Effects Of Seasonal Variation Of Abiotic Factors On Edna Detection Of Two Groundwater Crayfish Species, Jared Prine Higgs

Honors Capstone Projects and Theses

No abstract provided.

Pickle Associates With Histone Deacetylase 9 To Mediate Vegetative Phase Change In Arabidopsis, Tieqiang Hu, Darren Manuela, Valerie Hinsch, Mingli Xu

Faculty Publications

• The juvenile-to-adult vegetative phase change in flowering plants is mediated by a decrease in miR156 levels. Downregulation of MIR156A/MIR156C, the two major sources of miR156, is accompanied by a decrease in acetylation of histone 3 lysine 27 (H3K27ac) and an increase in trimethylation of H3K27 (H3K27me3) at MIR156A/MIR156C in Arabidopsis.

• Here, we show that histone deacetylase 9 (HDA9) is recruited to MIR156A/MIR156C during the juvenile phase and associates with the CHD3 chromatin remodeler PICKLE (PKL) to erase H3K27ac at MIR156A/MIR156C.

• H2Aub and H3K27me3 become enriched at MIR156A/MIR156C, and the recruitment of Polycomb Repressive Complex …

The Effect Of Physiographic Regions On Benthic Riffle Communities In The Kokosing River (Ohio), Cameron Brisbine, Gregory Brown, Jack Kessler

ONU Student Research Colloquium

Geology, physiographic history, and land use are among the most important factors influencing benthic community structure and distribution in surface waters, especially lotic systems. Given the higher till concentrations in glaciated regions, does this impact the quality of riffle habitats and associated benthic communities? The objective of this study was to examine the influence of glacial history on benthic stream communities and physicochemical variables of a river in central Ohio. The Kokosing River transitions through several physiographic regions within Ohio including the Till Plains, the Glaciated Allegheny Plateau, and the Unglaciated Allegheny Plateau. From 2020-2021, periphyton, macroinvertebrates, fish, and selected …

Study Proposal For Additional Investigation Of Epac1 In Endothelial Apoptosis, Anh Luu

ONU Student Research Colloquium

Inflammatory states such as sepsis are associated with elevated tumor necrosis factor alpha (TNF-⍺), which facilitates inflammation, increases the production of reactive oxygen species (ROS) and oxidative stress, and contributes to death of endothelial cells (EC). Exchange Factor Directly Activated by cAMP (EPAC1) is an intracellular cyclic-AMP (cAMP) sensor involved in EC responses such as barrier function, angiogenesis and proliferation. Previous studies in our lab demonstrated activation of EPAC1 protected EC from TNF⍺-induced cell death. Specifically, microvascular EC were pretreated for 45 minutes with 100 mM 8-pCPT-2′-O-Me-cAMP (8CPT, specific EPAC1 activator) or 10 mM forskolin (adenylyl cyclase activator that increases …

Chromatin Architecture: Mechanisms Of Gene Regulation, Logan O'Donnell

Honors Projects in Science and Technology

The rapid growth and division of cells as they proliferate and penetrate to surrounding tissues defines the collection of disease states known as cancer. Abnormal gene expression drives this uncontrollable replication of cells. In recent studies, aberrant HOX gene expression has been noted in a multitude of cancer types such as myeloid leukemia, prostate cancer, and breast cancer [1]. Within cancerous cells with aberrant HOX expression, late expressing HOX genes are suppressed while early expressing HOX genes are reactivated. HOX genes are significant in controlling early phases of organismal development such as cell cycle, cell movement, and gene expression. Likewise, …

Non-Native Coccinellid Beetles And Land Use Abundance Patterns In The Quad Cities Region, Jack Hughes, Tierney Brosius, Michael Reisner

Biology: Student Scholarship & Creative Works

Harmonia axyridis was introduced in 1916. This species of coccinellid beetle is considered a desirable species for agriculture practices because of its ability to control pests. There is increasing evidence that H. axyridis may be outcompeting native species which could be contributing to the decline of some species that are disappearing from the landscape. Harmonia axyridis has a large migration range of 2 Km enabling the species to affect a large area of land. Invasive species often increase in abundance in urban landscapes which may be the case with H. axyridis. During the summer of 2021 we investigated H. axyridis …

The Impact Of Supplemental Proline On Stress Tolerance In Cucumbers And Tomatoes, Wren Allison Jenkins

Honors Capstone Projects and Theses

No abstract provided.

Effects Of Urbanization On House Sparrow (Passer Domesticus) And House Finch (Haemorhous Mexicanus) Song Propagation, Sarah Grimes, Eliza Lewis

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

The recent surge in urbanization has increased pollution, which includes both physical pollution (e.g. exhaust) and sensory pollution (e.g., anthropogenic noise). We know birds increase the frequency and amplitude of their song in urban areas to reduce masking by low frequency noise pollution. However, bird song (i.e., a signal) is also affected by the environment and accompanying ambient noise. This study investigates how anthropogenic disturbances alter the ability of birds to communicate. Specifically, we aim to understand how urbanization affects the propagation of bird song by examining differences in active space, or the maximum distance a receiver can detect a …

Changes In Chromatic Contrast Of Avian Plumage In Forests With Different Levels Of Deer Browsing, Morgan Sherrard, Suihnem Mawi, Timothy Boycott, Megan Gall

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

Visual signal propagation through the environment can be influenced by many factors, including the visual background. For example, normally camouflaged animals can appear quite salient when the environmental substrate is changed. Deer can alter this visual background by consuming the forest understory; ultimately this can have implications for species that use visual signals to attract a mate or defend a territory. We are interested in studying how deer browsing affects the chromatic contrast (i.e., how much an animal stands out from the background for a given receiver) using an avian model system. Brown headed cowbirds (Molothrus ater) and wild turkeys …

The Effects Of Anthropogenic Disturbances On The Auditory Processing Of The House Sparrow (Passer Domesticus), Linda Nduwimana, Emma Yonker, Sarah Grimes, Suihnem Mawi, Eliza Lewis

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

Animal communication involves a sender producing a signal (e.g., vocalizations) that travels through the environment before being detected by a receiver. Although we know how senders can vary in signal production, relatively little is known about how receivers process these signals. The recent increase in urbanization can further complicate receiver sensory processing as anthropogenic activities (e.g., noise pollution) affects the way birds communicate. This study aims to investigate the influence of urbanization on the auditory processing system of house sparrows (Passer domesticus). House sparrows are an ideal model because they inhabit urban areas and rely on vocal cues from …

Sex Differences In Hormone-Modulated Neuroplasticity In The Songbird (Serinus Canarius), Paige A. Massa, Elianna E. Sandman, Anders P. Bogard, Matthew Czmer

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

In temperate zone species such as canaries (Serinus canaria) the neural circuitry regulating song behavior undergoes well-defined changes across the seasons. In males, increased daylengths in spring initiates an increase in gonadal volumes and circulating testosterone driving marked changes in brain morphology and song frequency. Females given exogenous testosterone in adulthood also demonstrate male-like changes in brain morphology and song behavior. More specifically, the telencephalic nucleus HVC (acronym is proper name), a key nucleus for song production, undergoes well-defined seasonal changes in neuroplasticity with a high rate of neurogenesis mediating marked changes in HVC volume. Much is known about the …

The Effect Of Serotonin On Males’ Neural And Behavioral Mechanisms To Female Ultrasonic Vocalizations And Urine In The House Mouse (Mus Musculus), Madisyn Kovacs

21st Annual Celebration of Undergraduate Research and Creative Activity (2022)

Animals communicate with multimodal signals (e.g., a mix of auditory, visual, or olfactory information) spanning several modalities; these signals may provide receivers with more complete information to allow for more accurate behavioral decisions. Past research suggests that serotonin plays a role in encoding multimodal social information (e.g., social partner presence) during communication events. Nevertheless, to our knowledge, no experiments have explicitly tested this hypothesis. 5-HTP, a precursor for serotonin, has been shown to increase serotonin in a region of the auditory midbrain, and is affected by social context. In our experiment, we asked the question: Does an increase in 5-HTP …

Assessing Environmental Factors That Influence Cyanobacterial Blooms In Skinn Lake, Kloe Atwood

Honors Projects

Global lakes are experiencing an increase in toxic algal blooms that can be damaging to the environment. These blooms are caused by cyanobacteria, specifically a species called Planktothrix. Skinn Lake, in Ohio, is a location of a cyanobacterial bloom that is mainly populated by Planktothrix rubescens. This lake was further examined to identify the major toxin-producing bacteria and identify environmental microbes surrounding the bloom. When the data was examined it was found that the microbiomes of the lake greatly differed between the winter (bloom) months and the summer (non-bloom) months. This difference can be viewed within the nutrient analysis from …

The Effect Of Estrogen On Gdnf Production Of Skeletal Muscle In Vitro, Hope Welter

Honors Theses

As life expectancy continues to rise, the number of people over age 65 in the U.S. is increasing. An aging population brings with it impacts on the national health system and associated economic expenditures. As individuals age, the skeletomuscular system suffers reduced function due to disease or conditions such as sarcopenia. Sarcopenia is characterized by muscular atrophy resulting from reduced muscle innervation. Exercise has been linked to prevention of sarcopenia, a suspected result of increased production of glial cell line-derived neurotrophic factor (GDNF). GDNF promotes neuronal survival, regeneration, and the formation of neuromuscular junctions. Therefore, treatments like exercise that increase …

Literature Review On Outer Hair Cell Regeneration And Potential Hearing Restoration, Serena Karlov

Honors Theses

The cochlea is the portion of the inner ear responsible for hearing, containing both the organ of Corti and auditory hair cells. Sound wave vibrations mechanically bend the stereocilia on the three rows of outer hair cells and one row of inner hair cells. These hair cells transduce this mechanical energy into electrical impulses that are relayed from the auditory nerve to the brain, where sounds are processed. In this review I provide an overview of inner ear anatomy, ear physiology, and auditory transduction. I then move on to specifically focus on the importance of outer hair cells, their regeneration, …

Metabolism, Cognition, And Male Coloration In Eastern Mosquitofish, Katelyn Ashleigh Giltner

Honors Capstone Projects and Theses

No abstract provided.

Food For Thought: The Relationship Between Nutrition And Academic Performance, Sarah Freeman

Honors Projects in Science and Technology

Nutrition has been shown to impact the level of cognitive function in individuals. Through the analysis of nutritional diets, this new research will aim to uncover the impact that diet has on cognitive function of Bryant University students by analyzing their ability to perform on a variety of cognitive assessments. This study will provide recommendations to students at Bryant University on what they should eat so they can perform their best academically. This research will also investigate whether Bryant University is offering the best food for students to eat setting them up for success.

Racial Health And Productivity Inequalities In The United States, Elissia Vecere

Honors Theses

Across America, it is frequently seen that there are may differences by race in economic and social outcomes. This variability can be seen in judicial, social, and economic systems. The breakdown of economic systems presenting racial variability can be displayed via workplace statistics. There is a general discrepancy between the workplace statistics represented with Black and White employees. These differences frequently are blamed on the productivity and background education supplied by the employee upon being hired. However, the levels of variation across these two races varies minutely in comparison to the differences in wages supplied. When all factors are kept …

The Effects Of Paclitaxel On Cellular Migration And The Cytoskeleton, Ashley Salguero-Gonzalez

Thinking Matters Symposium

In a clinical setting, some patients are exposed to an anti-cancer chemotherapy agent, paclitaxel. Cancerous cells undergo rapid, continuous cell division without control. Chemotherapy treatments try to slow and stop the uncontrollable cell division cycles and eliminate cancerous cells in the process. Paclitaxel serves as a treatment for some types of cancers, including lung, melanoma, bladder, and esophageal. Because it targets the cytoskeleton, paclitaxel can also influence cell migration. This project utilizes a cellular migration assay and an immunohistochemistry assay to analyze the effects of paclitaxel on the movement of cells and on the cytoskeleton of neuroglia rat cells with …

Laurier Biology Departmental Seminars Poster Colouring Book (Vol 1), Tristan A.F. Long

Biology Faculty Publications

Departmental seminar posters are largely utilitarian affairs – who is talking?, when are they speaking?, where is the seminar? (and maybe will there will be refreshments served?). While functional, they often fail to capture the beauty and excitement of their subject matter, and may fail to engage a wider audience. As the organizer of the Wilfrid Laurier University Department of Biology seminar series in 2014, on a lark, I started drawing posters that tried to illustrate our speaker’s research in a non-conventional manner, and hopefully appeal to those that might otherwise find attending a departmental seminar intimidating. Little did I …

Volume 13, Payton Davenport, Audrey Lemons, Jacob Shope, Haley Smith, Cassandra Poole, Rachel Cannon, Rachel Boch, Suzanne Stetson

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Roger A. Byrne, Dean

From the Editor Dr. Larissa “Kat” Tracy

From the Designers Rachel English, Rachel Hanson

The Effect of Compliment Type on the Estimated Value of the Compliment by Payton Davenport, Audrey Lemons, and Jacob Shope

The Imperial Japanese Military: A New Identity in the Twentieth Century, 1853–1922 by Haley Smith

Longwood University’s campus: Human-cultivated Soil has Higher Microbial Diversity than Soil Collected from Wild Sites by Cassandra Poole

Reminiscent Modernism: Poetry Magazine’s Modernist Nostalgia for the Past by Rachel Cannon

Challenges Faced by Healthcare Workers During the COVID-19 Pandemic: A Preliminary Study of Age and …

Skin Decision: Northern Fowl Mite Infection Alters Diversity Of The Skin Microbiome In European Starling Nestlings Differentially With Age, Kaitlin Couvillion

Honors Capstones

No abstract provided.

Grazing Influences Biomass Production And Protein Content Of Alpine Meadows, Laia Jarque-Bascuñana, Juan Antonio Calleja, Miguel Ibañez, Jordi Bartolomé, Elena Albanell, Johan Espunyes, Arturo Gálvez-Cerón, Josep María López-Martín, Miriam Villamuelas, Diana Gassó, Xavier Fernández-Aguilar, Andreu Colom-Cadena, Jennifer Adams Krumins, Emmanuel Serrano

Department of Biology Faculty Scholarship and Creative Works

Alpine grasslands are essential for carbon sequestration and food supply for domestic and wild herbivores inhabiting mountainous areas worldwide. These biomes, however, are alternatively threatened by the abandonment of agricultural and livestock practices leading to a fast-growing shrubification process while other mountain grasslands are suffering from the impacts of overgrazing. The functioning of alpine meadow ecosystems is primarily driven by climatic conditions, land-use legacies and grazing. However, although it is critically important, the role of large herbivores on the aboveground biomass and protein content of palatable plants is poorly understood for most alpine meadows. In this work, we explore the …