Biology Commons^™

The Abundance, Geographical Distribution And Habitat Use Of An Introduced Patas Monkey (Erythrocebus Patas) Population In Southwest Puerto Rico, Magaly Massanet

Graduate Theses

The southwest portion of Puerto Rico is home to introduced free-ranging populations of patas (Erythrocebus patas) and rhesus macaque (Macaca mulatta) monkeys. The rhesus macaque population originated from a colony established in 1961 on the islands of Guayacán and Cuevas off the southwest coast of Puerto Rico as part of the La Parguera Primate Research Center, which was administered by the Caribbean Primate Research Center. The descendants of these escapees have established free-ranging introduced monkey populations in mainland southwest Puerto Rico and the only previous study done on their ecology was conducted from 1990 to 1993 by González- Martínez (1995). …

Effects Of Ecological Restoration Techniques In Glen Canyon National Recreation Area, Simone Ka-Voka Jackson

UNLV Theses, Dissertations, Professional Papers, and Capstones

A better understanding of key ecological restoration techniques can inform land management in the Southwest on restoration options for areas infested by invasive grasses that can pose threats to ecosystems, from changes in nutrient cycling to altered fire regimes. In the semi-arid desert of Glen Canyon National Recreation Area (GLCA), several exotic grasses pose risks to local ecosystems: Saccharum ravennae, a relatively new invasive perennial grass, and Bromus rubens and Bromus tectorum, widespread annual grasses. In this study, multiple ecological restoration techniques were implemented to assess their effects on native and nonnative vegetation on sites invaded by the non-native grasses …

Assessing Minimal-Input Restoration Strategies For Desert Soil And Vegetation Restoration, Audrey Jean Rader

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Mojave and Sonoran Deserts have been negatively impacted by anthropogenic disturbances. Considering that these ecosystems may recover on millennial timescales, research has shown that restoration techniques can be fairly successful in initiating long-term recovery processes in these sensitive environments. However, uncertainty remains as to which techniques are effective in different circumstances, such as in different climates or across different soil properties, and which techniques may best avoid unintended consequences, such as facilitating non-native plants. To reduce fugitive dust as a human health hazard, increase soil stability, and enhance wildlife habitat, further work is necessary to develop restoration techniques for …

Tree To Grass Water Use Ratios; Assessing Turfgrass’ High Water Use In The Urban Landscape, Tamara Wynne

UNLV Theses, Dissertations, Professional Papers, and Capstones

Water demand in the southwestern United States continues to rise. The population of the Las Vegas Valley doubled from 2000-2010 and now more than two million people call it home. The residential sector uses 60% of all water consumed in the valley. Outdoor urban landscape irrigation is responsible for 70% of all residential use. These landscapes are dominated by trees and turf grass. Although the water use of turf grass species is well studied, there are few published results about the water use of landscape trees in the desert southwest USA. To obtain a more complete picture of the tradeoffs …

Defects In Fetal Mouth Movement And Pharyngeal Patterning Underlie Cleft Palate Caused By Retinoid Deficiency., Regina Friedl

Electronic Theses and Dissertations

Cleft palate is a common birth defect. Etiologic mechanisms of palate cleft include defects in palate morphogenesis, mandibular growth, or spontaneous fetal mouth movement. Cleft palate linked to deficient fetal mouth movement has been demonstrated directly only in a single experimental model of loss of neurotransmission. Here, using retinoid deficient mouse embryos, we demonstrate directly for the first time that deficient fetal mouth movement and cleft palate occurs as a result of mis-patterned development of pharyngeal peripheral nerves and cartilages. Retinoid deficient embryos were generated by inactivation of retinol dehydrogenase 10 (Rdh10), which is critical for production of …

Bayesian Inference Of Hydraulic Properties In And Around A White Fir Using A Process-Based Ecohydrologic Model, Elias C. Massoud, A. J. Purdy, Bradley O. Christoffersen, L. S. Santiago, C. Xu

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

We present a parameter estimation study of the Soil-Tree-Atmosphere Continuum (STAC) model, a process-based model that simulates water flow through an individual tree and its surrounding root zone. Parameters are estimated to optimize the model fit to observations of sap flux, stem water potential, and soil water storage made for a white fir (Abies concolor) in the Sierra Nevada, California. Bayesian inference is applied with a likelihood function that considers temporal correlation of the model errors. Key vegetation properties are estimated, such as the tree's root distribution, tolerance to drought, and hydraulic conductivity and retention functions. We find …

Effects Of Experimental Forest Management On Avian Communities In The Missouri Ozarks, David R. Hollie

Electronic Theses & Dissertations

In recent decades, concern for migratory birds has stimulated research assessing the relationships between forest management and bird populations. The Missouri Ozark Forest Ecosystem Project (MOFEP) is a long-term, landscape-scale experiment designed to examine the effects of even-aged (i.e. clearcutting), uneven-aged (i.e. selection cutting), and no harvest forest management on ecosystem level processes. The management systems were randomly assigned to three sites each (mean area = 400 ha) under a 100-year rotation with a 15-year re-entry period.

In the first chapter, we used non-metric multidimensional scaling and generalized linear mixed models to investigate the effects of silvicultural treatment and year-since-harvest …

Getting At The Surface: A Promoter And Coding Sequence Characterization Of An Odorant Receptor, Irena G. Parvanova

Dissertations, Theses, and Capstone Projects

Odorant receptors (ORs) are expressed by mature olfactory sensory neurons (OSNs), in a monogenic and monoallelic fashion (singular gene choice). It has been repeatedly published that the singular expression of ORs is a consequence of epigenetic regulation. The model invokes the depositing of epigenetic marks (off marks) that reflect transcriptional inactivity on all OR genes in immature neurons followed by the activation of one OR allele in maturing neurons. OR expression has been exclusively studied via transgenic animal models, due to the lack of cell line system that can undergo singular gene choice. Here, we used chromatin immunoprecipitation-quantitative polymerase chain …

The Role And Regulation Of Alternative Polyadenylation In The Dna Damage Response, Michael R. Murphy

Dissertations, Theses, and Capstone Projects

Cellular homeostasis is achieved by the dynamic flux in gene expression. Post-transcriptional regulation of coding and non-coding RNA offers a fast method of adapting to a changing cellular environment, including deadenylation, microRNA (miRNA) pathway, and alternative polyadenylation (APA). In this dissertation, I explored some of the mechanisms involved in the post-transcriptional regulation of gene expression. The main hypothesis in these studies is that a single APA event after DNA damage is governed by specific conditions and factors outside of current known regulators of APA, and that the resultant transcript has a role in the DNA damage response (DDR). My aims …

A Framework To Guide Undergraduate Education In Interdisciplinary Science, Brie Tripp, Erin E. Shortlidge

Biology Faculty Publications and Presentations

An expanded investment in interdisciplinary research has prompted greater demands to integrate knowledge across disciplinary boundaries. Vision and Change similarly made interdisciplinary expectations a key competency for undergraduate biology majors; however, we are not yet synchronized on the meaning of interdisciplinarity, making this benchmark difficult to meet and assess. Here, we discuss aspects of interdisciplinarity through a historical lens and address various institutional barriers to interdisciplinary work. In an effort to forge a unified path forward, we provide a working definition of interdisciplinary science derived from both the perspectives of science faculty members and scientific organizations. We leveraged the existing …

Differences Between Male And Female Elite Free-Style Wrestlers In The Effects Of “Set Up” On Leg Attack, Sho Ito, Larry Crawshaw, Kazuyuki Kanosue

Biology Faculty Publications and Presentations

Leg attack is the most basic skill in free-style wrestling. Before executing a leg attack, balance and anticipation of the opponent must be disrupted. We defined this action as the “set up”. The aim of this study is knowledge about the effects of “set up” on the success rate of leg attacks, and answer to question: whether or not a gender difference exists in the use and effectiveness of “set ups” in the elite level of the wrestling matches. Material and Methods: We analyzed video recordings of 57 matches of male free-style wrestling seniors, 58 matches of female seniors, 60 …

Vegetation Response To Intense Holocene Dry Events In The Andes And Amazon Basin, Majoi De Novaes Nascimento

Theses and Dissertations

By using the natural laboratory of past climate change I ask: How resilient are extant tropical forest ecosystems to climatic forcing? Do tropical forests systems show gradualistic or threshold responses to past climate change? How do climate forcings change floristic composition? And do humans amplify or suppress the effects of natural climate variability through land use? My study is set in western Amazonia and in the eastern flank of the Andes, regions renowned for high endemism and cultural importance. The time scale of the study spans the Mid-Holocene Dry Event (MHDE, c. 9000 to 4000 years ago), arguably the most …

The Incorporation Of Lipids Into The Cellular Membrane Of Salmonella, Betsy H. Redfern

Honors Theses

Salmonella is a gram negative, facultative anaerobic food borne pathogen and is the leading cause of deaths related to food borne illnesses. In order to establish an infection successfully, Salmonella must be able to survive in the presence of various stressors that it encounters, namely changes in pH, oxygen availability, osmolarity and bile. Previous research has shown that exposure to bile causes a shift in fatty acid composition in the cell membrane of the enteric bacterium Enterococcus faecalis. Thus, this led to the hypothesis that Salmonella incorporates fatty acids into its cellular membrane following exposure to bile and thereby protects …

The Molecular Mechanisms Underlying The Cancer Killing Effect Of Interleukin-24, Leah Eshanie Persaud

Dissertations, Theses, and Capstone Projects

Interleukin-24 (IL-24) is an immunomodulatory cytokine that also displays specific anti-tumor effects across many cancer cell types. The tumor suppressor activities of IL-24 include inhibition of angiogenesis, metastasis, toxic autophagy, cancer-specific apoptosis, and sensitization to traditional cancer treatments like chemotherapy and radiation. Overexpression of IL-24 can selectively induce apoptosis in various cancer cells while having no adverse effects on normal cells. Due to this favorable killing effect, IL-24 is currently in phase II clinical trials. There is accumulating evidence that IL-24’s anti-cancer activity is primarily through the endoplasmic reticulum (ER) stress pathway but other pathways leading to cell death are …

Color And Pigment Polymorphisms Of Northern Leopard Frogs On A Prairie Landscape, Kyle D. Gustafson, Bartholomew L. Bly, Robert A. Newman

Biology Faculty Publications

Variation allows populations to adapt to changing conditions. As human activities continue to alter environments and evolutionary processes, it becomes increasingly important to conserve standing genetic variation. Despite technical advances in population genetics, it is still useful to have inexpensive methods of detecting and monitoring genetic variation, particularly in traits that potentially influence fitness. In the Northern Leopard Frog, Lithobates pipiens (= Rana pipiens), genetically determined color (green [dominant: G] or brown [recessive: g]) and two pigment pattern polymorphisms (Burnsi/spotless [B] or spotted [b]; Kandiyohi/mottled [K] or non-mottled[k]) are hypothesized to have adaptive benefits. We assessed spatiotemporal patterns of these …

Health And Social Disparities Influence Opioid Misuse Among Adult Females Living In Rural Areas, Tequila Porter

Master of Science in Integrative Biology Theses

Since the early 1800s scientists have been working to create the best non-addictive pain medication derived from opium. In the early 1800s morphine was distilled from opium extracted from opium poppy plants (Quinones, 2015). Soon after a chemist invents a synthetic derivative of opium and names it diacetylmorphine (heroin) (Quinones, 2015, (Drugs and Morphine Text, 2018). Heroin originally was a safe cure for morphine addiction. Once this was proven to be false, and as having addictive properties, the government intervened and began initiatives to address the issue. This public crisis quickly grew to what modern scientists and healthcare professionals know …

What Does The Snake Eat? Breadth, Overlap, And Non-Native Prey In The Diet Of Three Sympatric Natricine Snakes, Emily E. Virgin, Richard B. King

Biology Faculty Publications

We investigated diet breadth and diet overlap in three sympatric snakes of similar body size: Dekay’s Brownsnakes (Storeria dekayi), Red-bellied Snakes (S. occipitomaculata), and sub-adult Common Gartersnakes (Thamnophis sirtalis), by examining recently consumed prey (n = 388) collected from wild-caught snakes (n = 263) in northern Illinois. Storeria occipitomaculata were dietary specialists, feeding nearly exclusively on slugs. Storeria dekayi fed predominately on slugs but also consumed snails and earthworms. Sub-adult T. sirtalis fed predominately on earthworms but also consumed frogs and small mammals. Diet overlap was extensive between Storeria species but relatively low between …

Short-Term Effect Of Simulated Salt Marsh Restoration By Sand-Amendment On Sediment Bacterial Communities, François Thomas, James T. Morris, Cathleen Wigand, Stefan M. Sievert

Faculty Publications

Coastal climate adaptation strategies are needed to build salt marsh resiliency and maintain critical ecosystem services in response to impacts caused by climate change. Although resident microbial communities perform crucial biogeochemical cycles for salt marsh functioning, their response to restoration practices is still understudied. One promising restoration strategy is the placement of sand or sediment onto the marsh platform to increase marsh resiliency. A previous study examined the above- and below-ground structure, soil carbon dioxide emissions, and pore water constituents in Spartina alterniflora-vegetated natural marsh sediments and sand-amended sediments at varying inundation regimes. Here, we analyzed samples from the …

Characterization Of Developmental Genes In Streptomyces, Erin Orr

Undergraduate Distinction Papers

Characterization of two developmental genes in Streptomyces was carried out by different genetic manipulation techniques in two different species. The construction of an overexpression mutant for a gene encoding a putative c-di-GMP binding protein in Streptomyces scabies was started. This gene was found to be a homologue of a protein that was found to bind to c-di-GMP in a protein binding assay that consisted of streptavidin coated beads and biotinylated c-di-GMP. Attempts at cloning the gene encoding this protein into an overexpression construct were made. Another developmental gene was also investigated. Gene disruption by PCR targeting was used to isolate …

Effects Of Polystrene Foam On The Growth And Development Of Darkling Beetles (Tenebrio Molitor), Nick Langlois

Undergraduate Distinction Papers

The growing field of entomoremediation explores the use of insects and other invertebrates as biological agents for the natural removal of hazardous substances, and recent research has investigated the potential for using mealworms (Tenebrio molitor) to digest and decompose polystyrene. Two experiments conducted over two years tested the tolerance of mealworm populations to the inclusion of polystyrene in their diet, and the ability of these diets to support multi-generational populations. The first experiment compared mealworms raised on a diet of 100% polystyrene foam with mealworms raised on a control diet of wheat bran and potatoes. The second experiment …

Characterization Of The Perilipin 5 C-Terminus, Tim D. Clinton

Undergraduate Honors Thesis Projects

The crystallization of protein with subsequent X-Ray crystallography analysis is a powerful technique for uncovering a protein’s structure. This methodology is cutting edge and innovations are being made daily on how to best use the fundamentals of this technique for clearer characterizations. The protein Perilipin 5 could benefit from this technique as properties involving the structure of the molecule are still largely unknown. Knowing the structural aspects of perilipin 5 is important as several research efforts indicate that is a key factor in the regulation of lipolysis, or the breakdown of fats within a biological system. In the life sciences, …

The Arylabialis Muscle Of The Túngara Frog (Engystomops Pustulosus), Amy D. Lagorio, Marcos Gridi-Papp

Pacific Research & Scholarship Day

No abstract provided.

Species Richness Patterns And Plant Size Of Vascular Epiphytes Along An Elevation Gradient In The Tropical Montane Forest Of Volcán Maderas, Nicaragua., Hazel Krupzkaya Berrios Alvarez

Student Theses and Dissertations

Aim To quantify distribution patterns of vascular epiphytes and determine how climate gradients drive species turnover patterns of tropical montane cloud forest systems. Location Volcán Maderas, Isla de Ometepe, Nicaragua. Methods Surveys were conducted in five distinct forest types as follows: Dry Forest, Humid Forest, Wet Forest, Cloud Forest, and Elfin Forest. Results A hump-shaped distribution pattern was detected for all vascular epiphytes at approx.1000 m in elevation (Cloud Forest). Overall species turnover was high for vascular epiphytes along the elevation gradient and was mainly driven by water availability. In addition, changes in size of vascular epiphytes were detected. Main …

Evaluating Centric Diatom Diversity In San Francisco Bay With Scanning Electron Microscopy, Ria Angelica Laxa, Jina Ham

Creative Activity and Research Day - CARD

Since September 2015, research has been conducted at the University of San Francisco (USF) to study planktonic diatoms in San Francisco Bay. Planktonic diatoms are one of the greatest contributors to the biodiversity in estuary systems. Due to their primary position in the food chain, changes in planktonic diatom communities will affect marine organisms at higher trophic levels. Therefore, the abundance and diversity of diatom species allows for the measurement of the health of the marine ecosystem. A previous study by Keith (2018), focused on documenting changes in species diversity over time, observing seasonal patterns in species richness as well …

Size Resistance To Infection With The Schistosome Parasite In The Vector Snail, Nicholas Tellechea, Mariam Banoub, Petros Minasi

Creative Activity and Research Day - CARD

Schistosomiasis is a parasitic disease that infects 200 million individuals, in mainly undeveloped countries, killing thousands. Schistosoma mansoni is one causative parasite, and it is transmitted by the freshwater snail Biomphalaria glabrata. B. glabrata snails of the BS-90 strain have shown neonatal susceptibility to the parasite, while adults are resistant, although the mechanism is unknown. Here we show that resistance to the parasite may be a function of snail size, since prevalence of infection is lower in larger snails, and since larval parasites (sporocysts) in larger snails have fewer germinal cells, are surrounded by a multilayered capsule of hemocytes, …

Differences In Thermal Niche Between Coastal And Inland Populations Of The Yellow Monkeyflower (Mimulus Guttatus), Alec J. Chiono, John R. Paul

Creative Activity and Research Day - CARD

Climate change will have multiple important impacts on coastal plant communities, yet how coastal plants will respond to temperature increases is understudied. Coastal areas experience small fluctuations in temperature daily and annually, similar to lowland tropical environments where research has shown that some tropical organisms are extremely threatened by increases in temperature because their thermal environmental has, historically, been very stable. Using multiple approaches, we plan to study if coastal and inland populations of the plant Mimulus guttatus differ in the evolution of their thermal niches. Are coastal populations more sensitive to changes in temperature, have coastal populations been limited …

Microfluidics On The Fly: Inexpensive Rapid Fabrication Of Thermally Laminated Microfluidic Devices For Live Imaging And Multimodal Perturbations Of Multicellular Systems, Megan Levis, Nilay Kumar, Emily Apakian, Ulises Hernandez, Ana Olivares, Fernando Ontiveros, Jeremiah J. Zartman

Biology Faculty/Staff Publications

Microfluidic devices provide a platform for analyzing both natural and synthetic multicellular systems. Currently, substantial capital investment and expertise are required for creating microfluidic devices using standard soft-lithography. These requirements present barriers to entry for many nontraditional users of microfluidics, including developmental biology laboratories. Therefore, fabrication methodologies that enable rapid device iteration and work “out-of-the-box” can accelerate the integration of microfluidics with developmental biology. Here, we have created and characterized low-cost hybrid polyethylene terephthalate laminate (PETL) microfluidic devices that are suitable for cell and micro-organ culture assays. These devices were validated with mammalian cell lines and the Drosophila wing imaginal …

Editorial: Invertebrate Models Of Natural And Drug-Sensitive Reward, Moira J. Van Staaden, Robert Huber

Biological Sciences Faculty Publications

The relationship between insects and humans is a complex one, characterized biotically as commensalism, mutualism, or parasitism. This collection of papers reveals yet another dimension, in which shared history invests invertebrate models with the power to interrogate critical challenges to the human reward system. As humans we take pride in approaching a given scenario rationally, in considering the possible options, assigning them values, and then choosing that which maximizes one's individual outcomes. So, why do drug addicts make choices that inevitably lead to ruinous consequences? Fundamentally, addiction appears to impair the very ability to form considered judgments, as it strips …

Presence And Distribution Of Leptodactylus Guianensis Heyer And De Sa, 2011 In Colombia: Comparisons With Other Species In The L. Latrans Group., Mariela Osorno Muñoz, Doris L. Gutiérrez-Lamus, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus guianensis is reported for the first time for Colombia along with a distributional map of the species. The species is the fifth species of the L. latransspecies group documented in the country. Males possess a single, conical and slightly chisel-shaped thumb spine; only three species in the group have a single thumb spine. In collections, specimens of L. guianensis are misidentified as L. macrosternum, L. latrans or L. bolivianus. We provide morphological, colouration and habitat descriptions for where the species occurs in Colombia.

Comparing Two Species: Historical Phylogeography And Species Delimitation Of Myiarchus Flycatchers In The West Indies., Emily L. Staden, Meghann Humphries, Leticia Soares, Kasey Fowler-Finn

Undergraduate Research Symposium

In a recent expedition to the Bahamas, the morphological observations of many of the individuals captured bore plumage with coloration that was characteristic of M. stolidus. A species that has up to this point, been considered allopatric. Documented distributions in the West Indies for Myiarchus flycatchers are indicative that while the M. stolidus inhabits Hispaniola and Jamaica, the Bahamas are the native habitat of M. sagrae. In a report from Joseph et al., questionable boundaries were discussed between the two species by highlighting that within a comprehensive Myiarchus phylogeny, M. sagrae is shown as paraphyletic, yet M. stolidus forms a …