Biology Commons^™

Regional Patterns Of Vascularity Within The Limb Bones Of Small And Large-Bodied Bats, Logan Usher

Williams Honors College, Honors Research Projects

Bats have humeri and femora that are poorly vascularized or avascular compared to birds, with only larger bats typically displaying vascular canals. Vascular canals provide passageways for blood vessels and nerves, which help maintain bone homeostasis, and support bones by resisting torsional stresses placed on them. The presence of a greater number of canals in large bats suggests that they may have a larger need for the support of nutrient and waste exchange than their dense lacunar-canalicular system can provide on its own. The focus of the current project will be to analyze SR micro-CT data from various bat humeri …

City Limits: Heat Tolerance Is Influenced By Body Size And Hydration State In An Urban Ant Community, Dustin Jerald Johnson

University of the Pacific Theses and Dissertations

Cities are rapidly expanding, and global warming is intensified in urban environments due to the urban heat island effect. Therefore, urban animals may be particularly susceptible to warming associated with ongoing climate change. Thus, I used a comparative and manipulative approach to test three related hypotheses about the determinants of heat tolerance or critical thermal maximum (CTmax) in urban ants—specifically, that (1) body size, (2) hydration status, and (3) preferred micro-environments influence CTmax. I further tested a fourth hypothesis that native species are particularly physiologically vulnerable in urban environments. I manipulated water access and determined CTmax for 11 species common …

Characterization Of The Roles Of Ppp1r15a (Gadd34) And Ppp1r15b (Crep) In Er Stress-Induced Apoptosis In Vivo, Danielle Hicks

University of the Pacific Theses and Dissertations

Failure to balance synthesis, folding and degradation of secreted proteins results in the accumulation of unfolded proteins in the endoplasmic reticulum, termed ER stress. Cells respond to ER stress by activating a signaling pathway known as the unfolded protein response (UPR). The UPR induces phosphorylation of eIF2α to attenuate global protein translation, allowing the ER to clear misfolded proteins. Opposing this function, eIF2α phosphatases contain a catalytic subunit, Protein Phosphatase 1, and either of two homologous scaffolding subunits, GADD34 and CReP. Inhibition of eIF2α phosphatases has been shown to prolong UPR signaling and promote survival in many cells types and …

Bat Species Diversity And Habitat Use Assessment With Focus On Endangered Indiana Bats In The Wright State University Woods, Megan R. Rude

Browse all Theses and Dissertations

The goals of my thesis are to: 1) identify species of bats in Wright State University’s (WSU) campus woods via acoustic surveys to compare to detections from previous years (Chapter 1) (2) analyze occupancy and detection probabilities of Indiana bats in different areas throughout the woods (Chapter 1), and 3) create an acoustical approach to analyze habitat use through bat social calls (Chapter 2). In Chapter 1, I conducted stationary acoustic surveys in the Wright State University woods in hydric (riparian), edge, and old growth habitats to record bat vocalizations. The WSU woods have a diverse bat community as ten …

Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae, John Kelly Dougherty

Browse all Theses and Dissertations

Two closely related Caenorhabditis species, C. briggsae and C. nigoni are cross fertile and produce viable adult progeny. From C. nigoni mothers, F1 adult females are viable and fertile, F1 males are viable but sterile. In crosses that utilize C. nigoni males and C. briggsae hermaphrodites produce viable adult F1 females but F1 males arrest during embryogenesis. A mutation in the Cbr-him-8 gene is a recessive maternal-effect suppressor of male-specific lethality. Hybrid crosses with cbr-him-8 mutant mothers produce viable adult male progeny. The HIM-8 protein in C. elegans is required for the pairing of X-chromosomes during meiosis. This function is …

The Effects Of Tumor Growth On Stress Response, Body Size, And Fat Content In Caenorhabditis Elegans, Reem Yassine

Senior Honors Theses and Projects

Tumorous growth affects nearby healthy tissues, as seen in wasting disease experienced by cancer patients, but the underlying mechanisms are not well understood. We studied tumor growth in C.elegans, a microscopic worm that shares many genes with humans and has the ability to grow tumors in its reproductive system. To test whether tumors cause stress in nearby tissues, we used genetic crosses to create strains containing tumors and fluorescent-based stress reporters for the following physiological stresses: endoplasmic reticulum unfolded protein response (hsp-4p::GFP), heat shock (hsp-6.2p::GFP), and infection (irg-1p::GFP). We found that expression of …

Fragile Oceans, Synthetic Flotsam And Microbial Collaboration – Explorations In The Visual Communication Of The Plastic Crisis, Ivan Langesfeld

Pomona Senior Theses

Scientific evidence that the ocean plastic crisis is larger in scale and more sinister than previously thought continues to mount, but the rate of plastic production is only rising. What will it take to decisively turn the tide against plastic? We need scientists, politicians, and industry changemakers to continue producing knowledge and positive change in the industry, but we need to go further still. This thesis explores art as an alternative visual communication strategy with the capacity to encourage curiosity, empathy, and positive engagement with the issue of ocean plastics. The series of work explores bacterial bioluminescence as an artistic …

The Effects Of A Ketone Body On Synaptic Transmission, Alexandra Elizabeth Stanback

Theses and Dissertations--Biology

The ketogenic diet is commonly used to control epilepsy, especially in cases when medications cannot. The diet typically consists of high fat, low carb, and adequate protein and produces a metabolite called acetoacetate. Seizure activity is characterized by glutamate excitotoxicity and therefore glutamate regulation is a point of research for control of these disorders. Acetoacetate is heavily implicated as the primary molecule responsible for decreasing glutamate in the synapse; it is believed that acetoacetate interferes with the transport of glutamate into the synaptic vesicles. The effects on synaptic transmission at glutamatergic synapses was studied in relation to the ketogenic diet …

Ecology, Monogamy, And The Evolution Of Animal Families, Jacqueline Rae Dillard

Theses and Dissertations--Biology

Family-living has been recognized as a necessary prerequisite for the evolution of advanced cooperative societies, yet the evolutionary and ecological processes that drive the coupling of different forms of cooperation in family-based societies are still poorly understood. In my dissertation, I investigate the correlated evolution of parental care, monogamy, and cooperative breeding in a variety of family-based taxa. I explore the mating and social behavior of family-living beetles with incipient cooperation to better understand the factors driving these social traits. Specifically, I evaluate different causes of extra-pair mating in socially monogamous beetles, the potential benefits that young adult offspring may …

Intraspecific Variation In Dehydration Tolerance: Insights From The Tropical Plant Marchantia Inflexa, Rose A. Marks

Theses and Dissertations--Biology

Plants are threatened by global change, increasing variability in weather patterns, and associated abiotic stress. Consequently, there is an urgent need to enhance our ability to predict plant community dynamics, shifts in species distributions, and physiological responses to environmental challenges. By building a fundamental understanding of plant stress tolerance, it may be possibly to protect the ecological services, economic industries, and communities that depend on plants. Dehydration tolerance (DhT) is an important mechanism of water stress tolerance with promising translational applications. Here, I take advantage natural variation in DhT to gain a deeper insight into this complex trait. In addition, …

Sibling Sex, But Not Androgens, Shapes Phenotypes In Perinatal Common Marmosets (Callithrix Jacchus), Brett M. Frye, Lisa G. Rapaport, Michael W. Sears, Talia Melber, Suzette D. Tardif

Publications

When offspring share a womb, interactions among fetuses can impart lasting impressions on phenotypic outcomes. Such intrauterine interactions often are mediated by sex steroids (estrogens and androgens) produced by the developing fetuses. In many mammals, intrauterine interactions between brothers and sisters lead to masculinization of females, which can induce fitness consequences. Many litter-bearing primates, though, seem to escape androgen-mediated litter effects, begging why? Here, we investigated how the sex composition (i.e., same- or mixed-sex) of litters influences perinatal outcomes in the common marmoset monkey (Callithrix jacchus), using a combination of physiological, morphological, and behavioural assays. We hypothesized that …

Sibling Sex, But Not Androgens, Shapes Phenotypes In Perinatal Common Marmosets (Callithrix Jacchus), Brett M. Frye, Lisa G. Rapaport, Talia Melber, Michael W. Sears, Suzette D. Tardif

Publications

When offspring share a womb, interactions among fetuses can impart lasting impressions on phenotypic outcomes. Such intrauterine interactions often are mediated by sex steroids (estrogens and androgens) produced by the developing fetuses. In many mammals, intrauterine interactions between brothers and sisters lead to masculinization of females, which can induce fitness consequences. Many litter-bearing primates, though, seem to escape androgen-mediated litter effects, begging why? Here, we investigated how the sex composition (i.e., same- or mixed-sex) of litters influences perinatal outcomes in the common marmoset monkey (Callithrix jacchus), using a combination of physiological, morphological, and behavioural assays. We hypothesized that …

Ecophysiological Determinants Of Sexual Size Dimorphism: Integrating Growth Trajectories, Environmental Conditions, And Metabolic Rates, Marie-Claire Chelini, John P. Delong, Eileen A. Hebets

Eileen Hebets Publications

Sexual size dimorphism (SSD) often results in dramatic differences in body size between females and males. Despite its ecological importance, little is known about the relationship between developmental, physiological, and energetic mechanisms underlying SSD. We take an integrative approach to understand the relationship between developmental trajectories, metabolism, and environmental conditions resulting in extreme female-biased SSD in the crab spider Mecaphesa celer (Thomisidae). We tested for sexual differences in growth trajectories, as well as in the energetics of growth, hypothesizing that female M. celer have lower metabolic rates than males or higher energy assimilation. We also hypothesized that the environment in …

Physiological And Genomic Evidence That Selection On The Transcription Factor Epas1 Has Altered Cardiovascular Function In Highaltitude Deer Mice, Rena M. Schweizer, Jonathan P. Velotta, Catherine M. Ivy, Matthew R. Jones, Sarah M. Muir, Gideon S. Bradburd, Jay F. Storz, Graham R. Scott, Zachary A. Cheviron

School of Biological Sciences: Faculty Publications

Evolutionary adaptation to extreme environments often requires coordinated changes in multiple intersecting physiological pathways, but how such multi-trait adaptation occurs remains unresolved. Transcription factors, which regulate the expression of many genes and can simultaneously alter multiple phenotypes, may be common targets of selection if the benefits of induced changes outweigh the costs of negative pleiotropic effects. We combined complimentary population genetic analyses and physiological experiments in North American deer mice (Peromyscus maniculatus) to examine links between genetic variation in transcription factors that coordinate physiological responses to hypoxia (hypoxia-inducible factors, HIFs) and multiple physiological traits that potentially contribute to …

Investigating How Faculty Social Networks And Peer Influence Relate To Knowledge And Use Of Evidence-Based Teaching Practices, A. K. Lane, J. Skvoretz, J. P. Ziker, Brian A. Couch, B. Earl, J. E. Lewis, J. D. Mcalpin, L. B. Prevost, S. E. Shadle, Marilyn Stains

School of Biological Sciences: Faculty Publications

Background: Calls for science education reform have been made for decades in the USA. The recent call to produce one million new science, technology, engineering, and math (STEM) graduates over 10 years highlights the need to employ evidence-based instructional practices (EBIPs) in undergraduate STEM classes to create engaging and effective learning environments. EBIPs are teaching strategies that have been empirically demonstrated to positively impact student learning, attitudes, and achievement in STEM disciplines. However, the mechanisms and processes by which faculty learn about and choose to implement EBIPs remain unclear. To explore this problem area, we used social network analysis to …

Functional Characterization Of O-Acetyltransferase Wssi: Role In Bacterial Cellulose Acetylation Of Achromobacter Insuavis And Pseudomonas Fluorescens, Alysha Burnett

Theses and Dissertations (Comprehensive)

Cellulose biofilms consist of a community of bacterial cells encased in a self-produced matrix of polymers (e.g. exopolysaccharides, such as cellulose) that facilitate a firm adherence to surfaces. The acetylation (addition of an acetyl group on carbohydrates) is crucial in virulence; thereby in some cases allowing opportunistic bacteria to cause harmful diseases. Pertaining to this research, the wrinkly spreader (WS) genotype of Pseudomonas fluorescens colonizes the air-liquid interface on food, water sources and human tissue to form a robust biofilm with the ability to spread across surfaces. The composition of this biofilm largely consists of bacterial cellulose polymers …

Potential Impact Of Northern Resource Development On Aquatic Biota: Toxicity Of Chromium And Rare Earth Element Processing Reagent, So Yeon Choi

Theses and Dissertations (Comprehensive)

Chromite and rare earth element development was identified in the 2015 Canadian Federal budget as a significant opportunity, however, key data gaps exist regarding the environmental concerns related to these resource developments. Chromium is essential in the production of stainless steel, and no suitable substitute is known. Rare earth elements (REEs) are a series of metals that are composed of 15 lanthanides, as well as scandium and yttrium. Uses for REEs range from electronic devices (i.e. cell phones, computers, televisions) to magnets and controlling nuclear reactors. While commercial production of REEs signify a great economic opportunity for Canada, key data …

Factors Influencing Zooplankton Communities In Small Arctic Lakes, Northwest Territories, Jasmina Vucic

Theses and Dissertations (Comprehensive)

The Canadian Arctic has been warming at an unprecedented rate, causing significant changes to the environment. At the same time, continued development in the north has increased the demand for gravel extraction used to construct and maintain infrastructure such as highways. The development of roadways and gradual loss of permafrost in Canada’s north has led to changes in water quality, including increased calcium, conductivity, and nutrients. In addition, gravel extraction has led to the formation of artificial gravel pit lakes. Research has yet to determine how physical and chemical changes associated with development and permafrost thaw might impact zooplankton, and …

Demographic Response Of The Gambian Gerbil To Seasonal Changes In Savannah Fallow Fields, Safianu Rabiu, Robert K. Rose

Biological Sciences Faculty Publications

The Savannah gerbil, Gerbilliscus gambianus (Muridae: Gerbillinae) is important to the ecological relations of the dry grassland ecosystem of West Africa, as well as, being a zoonotic agent of human diseases and po-tential crop pest. We examined the impact of seasonal changes on the population dynamics of G. gambianus in northern Nigeria, by completing population estimates using capture–mark–recapture (CMR) and indirect population density indices (PDI) methods. The latter included fecal pellet counts and limited spotlightening. During 1990–1992 we collected both CMR and PDI data, and established their relationship by regression, thus calibrating the PDI values to CMR estimator. We also …

Anti-C1q Autoantibodies Are Frequently Detected In Patients With Systemic Sclerosis Associated With Pulmonary Fibrosis, C. Liaskos, S. Rentouli, T. Simopoulou, A. Gkoutzourelas, G. L. Norman, A. Brotis, I. Alexiou, C. Katsiari, D. P. Bogdanos, L. I. Sakkas

Biological Sciences Faculty Publications

BACKGROUND: Anti-C1q autoantibodies (autoAbs) are associated with systemic lupus erythematosus (SLE) but their presence in other rheumatic diseases has not been adequately investigated.

OBJECTIVE: We aim to assessanti-C1q autoAbs and circulating immune complexes (CICs) in systemic sclerosis (SSc).

METHODS: One hundred twenty four patients with SSc (106 females; median age 59.4 years, range 25-81.4; 75(60.5%) with limited cutaneous SSc[lcSSc], and 49(39.5%) with diffuse cutaneous SSc[dcSSc]), were studied. Twenty-five patients with Sjögren's syndrome (SjS), 29 with rheumatoid arthritis (RA), and 38 patients with systemic lupus erythematosus (SLE) and 53 healthy controls (NC) were also included. ELISAs with high and low salt …

Exosomes Mediate Zika Virus Transmission Through Smpd3 Neutral Sphingomyelinase In Cortical Neurons, Wenshuo Zhou, Michael Woodson, Michael B. Sherman, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

The harmful effects of ZIKA virus (ZIKV) infection are reflected by severe neurological manifestations such as microcephaly in neonates and other complications associated with Guillain-Barre syndrome in adults. The transmission dynamics of ZIKV in or between neurons, or within the developing brains of the foetuses are not fully understood. Using primary cultures of murine cortical neurons, we show that ZIKV uses exosomes as mediators of viral transmission between neurons. Cryo-electron microscopy showed heterogeneous population of neuronal exosomes with a size range of 30-200 nm. Increased production of exosomes from neuronal cells was noted upon ZIKV infection. Neuronal exosomes contained both …

Prime-Boost Vaccine Regimen For Sjtpl And Sjc23 Schistosome Vaccines, Increases Efficacy In Water Buffalo In A Field Trial In China, Akram A. Da'dara, Changlin Li, Xinling Yu, Mao Zheng, Jie Zhou, Lisa M. Shollenberger, Yue-Sheng Li, Donald A. Ham

Biological Sciences Faculty Publications

Schistosomiasis remains a serious zoonotic disease in China and the Philippines. Water buffalo and cattle account for the majority of transmission. Vaccination of water buffalo is considered a key strategy to reduce disease prevalence. Previously, we showed that vaccination of water buffalo with SjC23 or SjCTPI plasmid DNA vaccines, induced 50% efficacy to challenge infection. Here, we evaluated several parameters to determine if we can develop a two dose vaccine that maintains the efficacy of the three dose vaccine. We performed four trials evaluating: (1) lab produced vs. GLP grade vaccines, (2) varying the time between prime and boost, (3) …

Clonally Expanded Alpha-Chain T-Cell Receptor (Tcr) Transcripts Are Present In Aneurysmal Lesions Of Patients With Abdominal Aortic Aneurysm (Aaa), Song Lu, John V. White, Raquel I. Judy, Lisa L. Merritt, Wan Lu Lin, Xiaoying Zhang, Charalambos Solomides, Ifeyinwa Nwaneshiudu, John Gaughan, Dimitri S. Monos, Emilia L. Oleszak, Chris D. Platsoucas

Biological Sciences Faculty Publications

Abdominal aortic aneurysm (AAA) is a life-threatening immunological disease responsible for 1 to 2% of all deaths in 65 year old or older individuals. Although mononuclear cell infiltrates have been demonstrated in AAA lesions and autoimmunity may be responsible for the initiation and account for the propagation of the disease, the information available about the pathogenesis of AAA is limited. To examine whether AAA lesions from patients with AAA contain clonally expanded α-chain TCR transcripts, we amplified by the non-palindromic adaptor-PCR (NPA-PCR)/Vα-specific PCR and/or the Vα-specific PCR these α-chain TCR transcripts. The amplified transcripts were cloned and sequenced. Substantial proportions …

Mapping Status And Conservation Of Global At-Risk Marine Biodiversity, Casey C. O'Hara, Juan Carlos Villaseñor-Derbez, Gina M. Ralph, Benjamin S. Halpern

Biological Sciences Faculty Publications

To conserve marine biodiversity, we must first understand the spatial distribution and status of at‐risk biodiversity. We combined range maps and conservation status for 5,291 marine species to map the global distribution of extinction risk of marine biodiversity. We find that for 83% of the ocean, >25% of assessed species are considered threatened, and 15% of the ocean shows >50% of assessed species threatened when weighting for range‐limited species. By comparing mean extinction risk of marine biodiversity to no‐take marine reserve placement, we identify regions where reserves preferentially afford proactive protection (i.e., preserving low‐risk areas) or reactive protection (i.e., mitigating …

Parasitic Dinoflagellate Hematodinium Perezi Prevalence In Larval And Juvenile Blue Crabs Callinectes Sapidus From Coastal Bays Of Virginia, H. J. Small, J. P. Huchin-Mian, K. S. Reece, K. M. Pagenkopp Lohan, Mark J. Butler Iv, J. D. Shields

Biological Sciences Faculty Publications

The parasitic dinoflagellate Hematodinium perezi infects the American blue crab Callinectes sapidus and other decapods along the Eastern seaboard and Gulf of Mexico coast of the USA. Large juvenile and adult blue crabs experience high mortality during seasonal outbreaks of H. perezi, but less is known about its presence in the early life history stages of this host. We determined the prevalence of H. perezi in megalopae and early benthic juvenile crabs from multiple locations along the Virginia portion of the Delmarva Peninsula. The DNA of H. perezi was not detected in any megalopae collected from several locations within …

Influence Of Ligand Complexation On Nickel Toxicity, Speciation And Bioavailability In Marine Waters, Samantha Sherman

Theses and Dissertations (Comprehensive)

Currently there are no site-specific bioavailability-based prediction models for assessing the impacts of nickel (Ni) in marine environments although there are indications that these may be warranted. The aim of this research was to characterize the complexation of Ni in relation to toxicity and speciation. Various complexing ligands were used, and it was predicted that the binding affinity (logKf) of ligands would be inversely correlated to toxicity based on dissolved Ni concentrations ([NiD]) but that on a free ion concentration ([Ni2+]) basis, toxicity would not vary. A two-phased approach was used; the first was a proof of principle where synthetic …

The Diversity Of Terrestrial Mammals Surrounding Waterfall At Billy Barquedier National Park, Kelsey Johnson, Jason Apple

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Billy Barquedier is a National Park located in the Stann Creek district of Belize that contains Neotropical vegetation and wildlife. This study was performed to provide a baseline inventory and appearance frequency patterns of the terrestrial mammals located within Zone 1 of the park near a waterfall and to gain a greater understanding of the biodiversity and activity patterns of terrestrial mammals within the park. The methods included camera traps, small Sherman live traps, large live traps, and tracking methods. A non-random sampling method of placing camera traps and live traps on or near human-made or animal-made trails was used …

Assessment Of Microbial Carbon Processing And Its Implications To The Carbon Budget Of Lake Superior, Lindsey Michelle Koren

Theses and Dissertations

Over the past few decades, there has been increased research focus on carbon cycling within aquatic systems, especially with the changing global climate. Inland waters play a major role in the global carbon cycle, but the fundamental features remain poorly understood, particularly the large lakes of the world. Our experimental approach assessing the carbon budget of Lake Superior, the largest freshwater lake by area, provides spatial and temporal variability that has been previously overlooked but may be critical to our understanding on the biogeochemical processes controlling the lake. Multiple stations were chosen across the lake, both nearshore and offshore, to …

Spatial Genetic Structure And Local Adaptation Within And Among Foxtail Pine (Pinus Balfouriana Subsp. Balfouriana) Populations Located In The Klamath Mountains, California, Rebecca D. Piri

Theses and Dissertations

Foxtail pine (Pinus balfouriana) is a subalpine conifer endemic to California, notably separated into two disjunct subspecies. Previous studies have described the northern subspecies,Pinus balfouriana subsp. balfouriana,as having an uncommonly high level of genetic differentiation and no discernible spatial patterns in phenotypic variation. This study seeks to characterize the spatial genetic structure and patterns of selection of the northern subspecies (Pinus balfouriana subsp. balfouriana) using genome-wide data and to identify the influence of ecology and environment on the unique genetic patterns. I show that genetic differentiation among populations is much less than previously estimated …

Preconditioning Of The Tumor Microenvironment By Means Of Low Dose Chemotherapies For An Effective Immunotherapy Of Breast Cancer, Hussein F. Aqbi

Theses and Dissertations

Breast cancer mortality is mainly due to distant recurrence of the disease arising from dormant tumor cells established by cancer therapies. Patients who initially respond to cancer therapies often succumb to distant recurrence of the disease. It is not clear why people with the same type of breast cancer respond to treatments differently; some escape from dormancy and relapse earlier than others. In addition, some tumor clones respond to immunotherapy while others do not. We investigated how autophagy plays a role in accelerating or delaying recurrence of neu overexpressing mouse mammary carcinoma (MMC) following adriamycin (ADR) treatment, and in affecting …