Biology Commons^™

The Role Of Ehd2 In Triple-Negative Breast Cancer Tumorigenesis And Progression, Timothy A. Bielecki

Theses & Dissertations

Triple-negative breast cancer (TNBC) comprises 10%-15% of all breast cancer cases, yet is clinically challenging due to lack of targeted therapies which leads to higher mortality. Molecular subtyping has identified the most aggressive subclasses of breast cancer to be enriched in components of caveolae. While caveolae have been linked to many biological processes, their precise role in TNBC is still poorly understood. EHD2, a member of the C-terminal EPS15-Homology Domain-containing (EHD) protein family, has emerged as a new regulator of caveolae dynamics and is essential to maintain a stable membrane pool of caveolae. Studies in model cells demonstrate that caveolae …

Hibernator And Non-Hibernator Responses To Acute Changes In Water Intake, Sydni S. Andruskiewicz

All College Thesis Program, 2016-2019

Hibernating animals undergo dramatic changes in metabolic rates during torpor. One of most notable changes in these animals is the ability to maintain blood pressure and perfuse certain organs. Consequentially, blood perfusion to the kidneys is greatly decreased and the ability to concentrate urine halts. However, about once a week, torpor is interrupted and the animal becomes active to rewarm itself about once a week. This activity induces rapid regeneration of the extracellular osmotic gradient of the kidney, and allows urine to be concentrated. Nonetheless, regaining the extracellular osmotic gradient creates a potentially fatal consequence to the kidney cells. To …

Scaling Of Anatomically Derived Maximal Bite Force In Primates, Ashley Deutsch

Senior Theses

Bite force analysis is important the examination of the evolutionary factors that have shaped the masticatory apparatus. Unfortunately, in primates, empirically measured bite force is largely unavailable because of the invasive nature of the procedure. Because of this, we have estimated bite force in a large sample across the primate order at three different realistic bite points along the tooth row: the first maxillary incisor, the maxillary canine, and the paracone of the first maxillary molar. Here, we model bite force by combining measured physiological crosssectional area of the jaw adductors from Hartstone-Rose and colleagues (2017) with osteological measurements from …

Differentiating The Neches River Rose Mallow (Hibiscus Dasycalyx) From Its Congeners By Means Of Phylogenetics And Population Genetics, Julia Norrell

Biology Theses

This study used molecular phylogenetic methods to attempt to resolve the taxonomic status of the federally threatened East Texas-endemic wildflower, the Neches River Rose Mallow (Hibiscus dasycalyx). Hibiscus dasycalyx co-occurs with two other closely related congeners that are currently not of conservation concern: the halberdleaf rose mallow (H. laevis); and the crimson-eyed rose mallow (H. moscheutos). This study assessed the phylogeny of these three Hibiscus species, and attempted to determine if there is possible hybridization occurring between them. To this end, Restriction Site Associated DNA Sequencing (RAD-Seq), a Next Generation Sequencing method, was used …

Hallmark Features Of Stem Anatomy In The Family Lamiaceae, Catherine Garner

Senior Theses

Lamiaceae is a cosmopolitan family, containing around 236 genera and 7,534 species. The family has been the object of considerable taxonomic controversy and ultimate revision, especially concerning subfamilial and tribal limits. A broad-based anatomical approach has been undertaken to assess comparative anatomy of the stem as a reasonable systematic technique. The stems of several members of the family were compared. Cross-sections were made using the recently invented hydromicrotome, fixed, and examined at magnifications of 40x, 100x, and 400x. The common anatomical features of each sectioned species were determined, and used to establish a basic guide to the hallmark stem features …

Epigenetic Regulation Of Salicylic Acid-Mediated Plant Defense, Phillip Key

Senior Theses

Plant pathogens remain a significant threat to the stability of modern agricultural systems, and the investigation of mechanisms to improve the security of food resources has led to the partial characterization of plant immune response. With this progress, there is a newfound ability to analyze the relative pathogen resistance capability of specifically modified organisms, and in doing so, it is possible to identify individual alterations that might play a role in creating a more robust immune response. In this study, ten Arabidopsis thaliana mutants were infiltrated with Pseudomonas syringae pv. morsprunorum alongside positive and negative control Col-0 Wild-Type and npr1-2. …

Rehydration From Desiccation: Evaluating The Potential For Leaf Water Absorption In X. Elegans, Mitchell Braun

Honors Thesis

Desiccation tolerance is the ability to survive through periods of extreme cellular water loss. Most seeds commonly exhibit a degree of desiccation tolerance while vegetative bodies of plants rarely show this characteristic. Desiccation tolerant vascular plants, in particular, are a rarity. Although this phenomenon may have potential benefits in crop populations worldwide, there are still many gaps in our scientific understanding. While the science behind the process of desiccating has been widely researched, the process of recovering from this state of stress, especially in restoring xylem activity after cavitation is still relatively unknown. Although plants normally gain water through their …

Assessing Variation In Visual Abilities In Daphnia As A Result Of Disparity In Eye Sizes, Adrian Perez

Senior Theses

The functionality of an eye is affected by a number of structural factors, and altering any of these factors often involves inevitable tradeoffs between resolution and sensitivity. However, it has been theoretically reasoned, and tested empirically, that increasing the size of an eye allows for both of these central features of vision to be improved due to corresponding increases in lens size. This study examines the relevance of this hypothesis for the vision of Daphnia in both interspecific and intraspecific contexts. The visual capabilities of six different species of Daphnia are tested using the optomotor response—a visually mediated behavior that …

An Illustrated Investigation Of Agraulis Vanillae And Its Larval Food Plants, Christiana M. Keinath

Senior Theses

To create my work, I directly observed caterpillars, chrysalises, and adult butterflies. I used a variety of media to depict salient aspects of gulf fritillary anatomy and to follow the species through its life stages in connection with its passionflower hosts. I focused on accurately recreating what I observed, as well as breaking down the organisms into their component colors, lines, shapes, and values. In my work I emphasized natural textures, which vary greatly as the caterpillars undergo their morphological changes. As a result of this project, I improved my observation and life drawing skills and learned how the process …

Tree Damage From Hurricane Matthew Affecting Forest Dynamics In Congaree National Forest, Connor Lentz

Senior Theses

The Congaree National Forest serves as an ecological model for bottomland-hardwood forests. Hurricanes periodically strike the Southeast United States, with high-wind speeds causing damage affecting the Congaree National Forest and modifying forest dynamics. This has been well-studied from the effects of Hurricane Hugo in September 1989 (Sharitz et al. 1993). In October 2016, Hurricane Matthew, a category 5 storm, made landfall in the Southeastern United States, and eventually moved through the Congaree National Forest. The high winds and precipitation caused widespread tree damage throughout Congaree. This study used a belt-transect survey of 14.7km of trails throughout the park to record …

Understanding The Past, Present, And Future Of Land Conservation In South Carolina, Nicole Berson

Graduate Theses

Urbanization poses a significant challenge for many ecosystems in the United States. However, monitoring its impacts requires extensive data and this lack of up-to-date information makes understanding the impacts of urbanization difficult to assess. One area that has seen tremendous growth is the Interstate 85 (I-85) corridor between Charlotte, NC and Atlanta, GA, which is known as “The Boom Belt.” Unfortunately, due to limited resources from conservation and state agencies, data on land use change and its impacts in this area have not been updated since the early 1990s. To investigate how urbanization is impacting this region, I conducted a …

Anti-Predator Behavior And Discriminative Abilities: Playback Experiments With Free-Ranging Equatorial Saki Monkeys (Pithecia Aequatorialis) In The Peruvian Amazon, Candace Stenzel

Graduate Theses

Anti-predator behaviors of free-ranging groups of equatorial saki monkeys (Pithecia aequatorialis) were recorded in the Área de Conservación Regional Comunal Tamshiyacu Tahuayo in the Peruvian Amazon to determine whether individuals responded in predator-specific ways to calls of aerial and terrestrial predators. Previous studies have shown that several species of Old World monkeys possess these discriminative abilities, but few have explored this question in New World monkeys. The ability to recognize predators and to respond appropriately is important for survival. Thus, we predicted that equatorial saki monkeys should respond in predator-specific ways, both vocally and behaviorally, to aerial and terrestrial predators. …

Distribution Of Plethodon Cinereus Color Morphs In New England: 40 Years Later, Mariah Fossella

Honors Program Theses and Projects

Global climate change is threatening species with habitat loss and extinction, but specific mechanisms driving changes in species abundance are unclear, partly due to a lack of time-series data sets documenting population changes. In order to study these effects on amphibian species, we revisited Lotter and Scott’s 1977 study of color morph frequency in relation to climate in Plethodon cinereus (eastern red-backed salamander). 25 Massachusetts, New Hampshire, and Vermont localities were resampled during summer 2016, to quantify any changes in morph frequency since 1977. At 19 of the 25 localities, we were able to find ≥100 salamanders, and of those …

Functional Analysis And Treatment Of Self-Injurious Feather Plucking In A Black Vulture (Coragyps Atratus), Kristen L. Morris

Thesis Projects

Feather plucking (FP) is a maladaptive behavior observed in captive avian species. This self-injurious behavior results in damage to and removal of feathers and skin tissue, resulting in animal welfare and financial consequences. The etiology and maintenance of FP have been hypothesized through medical and environmental processes, yet a definitive solution has not been found. The current study investigated the environmental variables maintaining the FP of a Black Vulture (Coragyps atratus), as well as evaluated a function-based treatment for this behavior. The behavior was found to be maintained by positive reinforcement in the form of contingent attention. Treatment …

The Effect Of Staphylococcus Aureus On Stiffness Of Cortical Bone, Ariel N. Kunde

Graduate Theses

Osteomyelitis, a term for bone infection, is a common cause of hospitalization in the United States. Infection leading to osteomyelitis is almost always a product of bacterial origin. Although polymicrobial presence is seen at infection sites of osteomyelitis, Staphylococcus aureus is most commonly isolated and found to be the cause of more than 95% of bone infection in adults. This organism is a common commensal of humans that is carried by an estimated 60% of the US population. S. aureus is transferred by infected asymptomatic individuals, and its ability to proliferate under a variety of environmental conditions contributes to the …

Genetic Bottlenecks In Pristis Sawfishes In Northern Australian Waters, Nicole M. Phillips, Annemarie Fearing, David L. Morgan

Faculty Publications

Northern Australia has been identified as the last stronghold for the dwarf sawfish Pristis clavata, green sawfish P. zijsron, and largetooth sawfish P. pristis, making these populations key in global conservation efforts for each species. This research assesses the levels of genetic diversity in these 3 sawfishes in Australian waters, testing for the presence of population bottlenecks using data at microsatellite loci. Levels of observed heterozygosity in each species from the west coast of Australia and the north-eastern Gulf of Carpentaria were generally high. M ratio tests suggest that assemblages of P. zijsron and P. pristis on …

Deer Droppings As A Diverse Invertebrate Habitat, Allison Furr, Dr. Kevin Geedey

Celebration of Learning

This project was performed at Augustana's Green Wing Environmental Laboratory and studied the richness and diversity of soil invertebrates in two conditions, deer poop enriched and surrounding soil. Soil invertebrates play an important role in soil quality which greatly affects ecosystems. The hypothesis of this study was that the diversity and richness would be the higher in the poop enriched soil samples. Soil samples were collected, Burlese funnels were used to collect the invertebrates, the invertebrates were examined under the microscope, and were assigned to taxa. The number of taxa (richness) and the number of individuals in each taxa (Simpson's …

Combined Consumption Of Alcohol And A High-Fat Diet: Effects On Behavior And Health In Male And Female C57b1/6j Mice, Rachel Gelineau

Honors Program Theses and Projects

Poor diets and alcohol are known to affect both behavior and overall health. Each by itself can produce an increase in anxiety and can affect insulin and leptin, important indicators of Type 2 Diabetes Mellitus. This project investigated how combined high-fat diet and access to alcohol affected feeding and drinking behaviors, anxiety-like behaviors, glucose tolerance, and leptin and insulin levels in C57BL/6J male and female mice. Mice were separated into three food groups: high-fat diet, low-fat diet, and regular chow and each group was paired with either water or forced 10%-alcohol. Weekly body weight, and food and drink intake measurements …

Beyond 3 Au From The Sun: The Hypervolatiles Ch4, C2h6, And Co In The Distant Comet C/2006 W3 (Christensen), Boncho Bonev, Geronimo Villanueva, Michael Disanti, Hermann Boehnhardt, Manuela Lippi, Erika Gibb, Lucas Paganini, Michael Mumma

Physics Faculty Works

Comet C/2006 W3 (Christensen) remained outside a heliocentric distance (R h) of 3.1 au throughout its apparition, but it presented an exceptional opportunity to directly sense a suite of molecules released from its nucleus. The Cryogenic Infrared Echelle Spectrograph at ESO-VLT detected infrared emissions from the three "hypervolatiles" (CO, CH4, and C2H6) that have the lowest sublimation temperatures among species that are commonly studied in comets by remote sensing. Even at R h = 3.25 au, the production rate of each molecule exceeded those measured for the same species in a number of other comets, although these comets were observed …

Analysis Of “No Net Loss” Wetland Mitigation Policy, Kristina Erickson

Honors Capstones

Wetlands in the United States have long been destroyed and degraded to make way for anthropogenic development. In recent history, efforts have been made to slow this degradation. The “no net loss” wetland mitigation policy has been in effect since 1991 and seeks to balance the needs of both industry and the environment. However, this policy is flawed. The regulatory process favors the interests of industry and the United States continues to lose wetlands and and their important functions. Therefore, the examination of the current policy is key to prevent future loss of wetland functions.

Chromatin-Modifying Agents Convert Fibroblasts To Oct4+ And Vegfr-2+ Capillary Tube-Forming Cells, Anita Wary, Neil Wary '18, Jugajyoti Baruah, Victoria Mastej, Kishore K. Wary

Student Publications & Research

Rationale
The human epigenome is plastic. The goal of this study was to address if fibroblast cells can be epigenetically modified to promote neovessel formation.

Methods and results
Here, we used highly abundant human adult dermal fibroblast cells (hADFCs) that were treated with the chromatin-modifying agents 5-aza-2'-deoxycytidine and trichostatin A, and subsequently subjected to differentiation by activating Wnt signaling. Our results show that these epigenetically modified hADFCs increasingly expressed β-catenin, pluripotency factor octamer-binding transcription factor-4 (OCT4, also known as POU5F1), and endothelial cell (EC) marker called vascular endothelial growth factor receptor-2 (VEGFR-2, also known as Fetal Liver Kinase-1). In microscopic …

From Sexless To Sexy: Why It Is Time For Human Genetics To Consider And Report Analyses Of Sex, Matthew S. Powers, Phillip H. Smith, Sherry A. Mckee, Marissa A. Ehringer

Publications and Research

Science has come a long way with regard to the consideration of sex differences in clinical and preclinical research, but one field remains behind the curve: human statistical genetics. The goal of this commentary is to raise awareness and discussion about how to best consider and evaluate possible sex effects in the context of large-scale human genetic studies. Over the course of this commentary, we reinforce the importance of interpreting genetic results in the context of biological sex, establish evidence that sex differences are not being considered in human statistical genetics, and discuss how best to conduct and report such …

Females Are The Brighter Sex: Differences In External Fluorescence Across Sexes And Life Stages Of A Crab Spider, Erin E. Brandt, Susan E. Masta

Biology Faculty Publications and Presentations

Fluorescence is increasingly recognized to be widespread in nature. In particular, some arachnids fluoresce externally, and in spiders the hemolymph fluoresces. In this study, we examined the external fluorescence and the fluorophores of different sexes and life stages of the crab spider Misumena vatia (Clerk 1757), a sit-and-wait predator that feeds on insects as they visit flowers. We designed novel instrumentation to measure external fluorescence in whole specimens. We found that although males and females possess internal fluorophores with similar properties, the external expression of fluorescence varies across sexes and life stages. Spiders fluoresce brightly as immatures. Females maintain their …

Transciptome Analysis Illuminates The Nature Of The Intracellular Interaction In A Vertebrate-Algal Symbiosis, John A. Burns, Huanjia Zhang, Elizabeth M. Hill, Eunsoo Kim, Ryan R. Kerney

Biology Faculty Publications

During embryonic development, cells of the green alga Oophila amblystomatis enter cells of the salamander Ambystoma maculatum forming an endosymbiosis. Here, using de novo dual-RNA seq, we compared the host salamander cells that harbored intracellular algae to those without algae and the algae inside the animal cells to those in the egg capsule. This two-by-two-way analysis revealed that intracellular algae exhibit hallmarks of cellular stress and undergo a striking metabolic shift from oxidative metabolism to fermentation. Culturing experiments with the alga showed that host glutamine may be utilized by the algal endosymbiont as a primary nitrogen source. Transcriptional changes in …

Ancient Bacteria–Amoeba Relationships And Pathogenic Animal Bacteria, Joan E. Strassmann, Longfei Shu

Biology Faculty Research

Long before bacteria infected humans, they infected amoebas, which remain a potentially important reservoir for human disease. Diverse soil amoebas including Dictyostelium and Acanthamoeba can host intracellular bacteria. Though the internal environment of free-living amoebas is similar in many ways to that of mammalian macrophages, they differ in a number of important ways, including temperature. A new study in PLOS Biology by Taylor-Mulneix et al. demonstrates that Bordetella bronchiseptica has two different gene suites that are activated depending on whether the bacterium finds itself in a hot mammalian or cool amoeba host environment. This study specifically shows that B. …

Comparison Of The Regulatory Dynamics Of Related Small Gene Regulatory Networks That Control The Response To Cold Shock In Saccharomyces Cerevisiae, Natalie Williams

Honors Thesis

The Dahlquist Lab investigates the global, transcriptional response of Sacchromyces cerevisiae, baker’s yeast, to the environmental stress of cold shock, using DNA microarrays for the wild type strain and strains deleted for a particular regulatory transcription factor. Gene regulatory networks (GRNs) consist of transcription factors (TF), genes, and the regulatory connections between them that control the resulting mRNA and protein expression levels. We use mathematical modeling to determine the dynamics of the GRN controlling the cold shock response to determine the relative influence of each transcription factor in the network. A family of GRNs has been derived from the …

Risc-Interacting Clearing 3’- 5’ Exoribonucleases (Rices) Degrade Uridylated Cleavage Fragments To Maintain Functional Risc In Arabidopsis Thaliana, Zhonghui Zhang, Fuqu Hu, Min Woo Sung, Chang Shu, Claudia Castillo-González, Hisashi Koiwa, Guiliang Tang, Martin Dickman, Pingwei Li, Xiuren Zhang

Michigan Tech Publications, Part 1

RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5’ and 3’ RNA fragments. The 5’ cleaved RNA fragments are marked with uridylation for degradation. Here, we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2. RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo. RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits. Notably, ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; …

Why Do Curly Tail Lizards (Genus Leiocephalus) Curl Their Tails? An Assessment Of Displays Toward Conspecifics And Predators, Bonnie K. Kircher, Michele A. Johnson

Biology Faculty Research

Animal display behaviors are used to convey specific messages to other animals, including potential mates, rivals, and predators. However, because these different types of interactions can be mediated by a single behavioral display, or conversely, multiple signals can be used to convey one specific message, interpretation of any particular behavioral display can be difficult. Leiocephalus lizards (i.e., curly tails) provide an excellent opportunity to study the use of display behaviors across multiple contexts. Previous research has demonstrated that the use of tail curling in these lizards is associated with predation risk, but less is known regarding the use of this …

Identification And Characterization Of Endophytic Fungi Associated With The Leaves Of Moringa Oleifera Lam, Vivian A. Panes, E.S Carbungco, N.B Pedroche, T.E De La Cruz

Biology Faculty Publications

Fungal endophytes live within host plants and are recently gaining interest as sources of biologically active secondary metabolites. In this research, fungal endophytes associated with leaves of Moringa oleifera Lam. were isolated, characterized and identified. Leaf samples from two moringa trees were collected from Barangay Pandan, which is an urban area and Barangay Sapang Bato considered as a rural area with the highest elevation of all the barangays of Angeles City. All leaf samples were rid of debris by rinsing with tap water. A flame-sterilized one-hole puncher was used to bore 54 explants from leaves collected from each tree of …

The Effects Of Various Therapeutics On Cystine Stone Formation, See Yang

Dissertations, Masters Theses, Capstones, and Culminating Projects

Cystinuria is an autosomal recessive genetic disorder characterized by the defect of a renal transporter involved in cystine reabsorption. When this transporter is deficient, cystine cannot be broken down and reabsorbed by the body and is excreted via urine in high concentrations. The high levels of cystine present in the urine eventually lead to recurrent cystine urolithiasis due to its inability to solubilize. Despite having various forms of treatments such as thiol pharmaceutical therapies such as tiopronin and urine alkalinizing agents like potassium citrate, only few patients with cystinuria are able to successfully decrease cystine urine concentration. We observed the …