Biology Commons^™

Biology News, Winter 2017, University Of Northern Iowa. Department Of Biology.

Biology News

Contents:

--Greetings From the Department of Biology
--Department of Biology Alumni
--Kelly Bahl Interns at Epcot in Disney World
--Former Student, Susann Ahrabi-Fard, Working With the Zika Virus
--Department News
--Study Abroad Course to the Galapagos
--UNI Maintains Strength in Undergraduate Research
--Biology Faculty Incorporate Their Artistic Skills Into the Classroom and Publications
--Faculty Retirement
--Alumni In Residence
--University of Northern Iowa Awarded Grant Through the Roy J. Carver Foundation
--Reconnect With the Department of Biology
--You Can Make a Difference
--Scholarships and Funding

Uncovering Candidate Cold Tolerance Genes In Maize (Zea Mays), Raeann N. Goering

Departmental Honors Projects

With booming populations soon to overwhelm the world's food production capabilities, studying what makes crop organisms, like maize, efficient is crucial to ensure that the demand for food is met. Planting earlier in the spring would lengthen the crop season and produce larger yields provided the crop is tolerant to early spring’s low temperatures. Plants can adjust to abiotic stresses through biochemical changes controlled by transcription of genes. Trained plants can be produced by pre-exposing them to a lesser stress, allowing them to recover, then exposing them to a greater, longer stress. It is hypothesized that trained plants will tolerate …

Analysis Of Ca125 Antigen In Normal Human Seminal Plasma Highlightsthe Molecular Heterogeneity Of Underlying Glycosylated Species, Ninoslav Mitic, Bojana Milutinovic, Miroslava Jankovic

Turkish Journal of Biology

Cancer antigen CA125 represents an extracellular part of transmembrane mucin MUC16 and may be expressed in tissues of the male reproductive tract. It reaches human seminal plasma (hSP) after undergoing the main processes involved in protein speciation: synthesis, posttranslational modifications, compartmentalization, and auto/proteolytic degradation. This study was aimed at profiling CA125-immunoreactive species in hSP from healthy subjects as a specific and unexplored source of this tumor-associated antigen expressed under normal physiological conditions. High molecular mass components from total hSP and corresponding acid-soluble hSP preparations were analyzed. The results indicated that antibodies recognizing two distinct immunogenic areas of CA125 antigen exhibited …

Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan

Turkish Journal of Biology

Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …

Microbiological Interactions With Cold Plasma, Paula Bourke, Dana Ziuzina, Lu Han, Patrick J. Cullen, Brendan Gilmore

Articles

ere is a diverse range of microbiological challenges facing the food, healthcare and clinical sectors. The increasing and pervasive resistance to broad-spectrum antibiotics and health-related concerns with many biocidal agents drives research for novel and complementary antimicrobial approaches. Biofilms display increased mechanical and antimicrobial stability and are the subject of extensive research. Cold plasmas (CP) have rapidly evolved as a technology for microbial decontamination, wound healing and cancer treatment, owing to the chemical and bio-active radicals generated known collectively as reactive oxygen and nitrogen species. This review outlines the basics of CP technology and discusses the interactions with a range …

Highly Branched Poly(5-Amino-1-Pentanol-Co-1,4- Butanediol Diacrylate) For High Performance Gene Transfection, Ming Zeng, Dezhong Zhou, Singwei Ng, Jonathan O'Keefe Ahern, Fatma Alshehri, Yongsheng Gao, Luca Pierucci, Udo Greiser, Wenxin Wang

Articles

The top-performing linear poly(β-amino ester) (LPAE), poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (C32), has demonstrated gene transfection efficiency comparable to viral-mediated gene delivery. Herein, we report the synthesis of a series of highly branched poly(5-amino-1-pentanol-co-1,4-butanediol diacrylate) (HC32) and explore how the branching structure influences the performance of C32 in gene transfection. HC32 were synthesized by an “A2 + B3 + C2” Michal addition strategy. Gaussia luciferase (Gluciferase) and green fluorescent protein (GFP) coding plasmid DNA were used as reporter genes and the gene transfection efficiency was evaluated in human cervical cancer cell line (HeLa) and human recessive dystrophic epidermolysis bullosa keratinocyte (RDEBK) cells. We …

Evaluation Of Chemical Immersion Treatments To Reduce Microbial Populations In Fresh Beef, Ahmed Kassem, Joseph Meade, James Gibbons, Kevina Mcgill, Ciara Walsh, James Lyng, Paul Whyte

Articles

The aim of the current study was to assess the ability of a number of chemicals (acetic Acid (AA), citric acid (CA) lactic acid (LA), sodium decanoate (SD) and trisodium phosphate (TSP)) to reduce microbial populations (total viable count, Campylobacter jejuni, Escherichia coli, Salmonella typhimurium and Listeria monocytogenes) on raw beef using an immersion system. The following concentrations of each chemical were used: 3 & 5% for AA, CA, LA, SD and 10 & 12% for TSP. Possible synergistic effects of using combinations of two chemicals sequentially (LA +CA and LA +AA) were also investigated. L*, a* and b* values …

Literature Record Checklist Of True Bugs (Hemiptera) For Arkansas, U.S.A., As Of 2018., Stephen W. Chordas Iii

Journal of the Arkansas Academy of Science

No abstract provided.

An Annotated Checklist Of The Crayfishes (Decapoda: Cambaridae) Of Arkansas, Henry W. Robison, Keith A. Crandall, Chris T. Mcallister

Journal of the Arkansas Academy of Science

Prior to the present study, 56 species with 3 additional subspecies for a total of 59 different taxa of crayfishes were recognized from Arkansas. We add a single species (Carmel Crayfish, Fallicambarus schusteri) to that list, subtract a documented synonym (Procambarus ferrugenius = Procambarus liberorum), update the classification to better reflect recent phylogenetic insights, and provide an updated annotated checklist of the 59 crayfish taxa of presently known from the state. There are 8 endemic species in Arkansas, including the Bayou Bodcau Crayfish (Bouchardina robisoni), Boston Mountains Crayfish (Cambarus causeyi), Hell Creek …

Leech Parasitism Of The Gulf Coast Box Turtle, Terrapene Carolina Major (Testudines:Emydidae) In Mississippi, Usa, Dennis J. Richardson, C. I. Hammond, W. E. Moser, A. J. Heaton, C. T. Mcallister

Journal of the Arkansas Academy of Science

Ten leeches were collected from a Gulf Coast box turtle, Terrapene carolina major, found crossing a road in Gulfport, Harrison County, Mississippi, USA. Eight of the leeches were identified as Placobdella multilineata and 2 were identified as Helobdella europaea. This represents the second vouchered report of leeches from a box turtle. Helobdella europaea is reported for the first time associated with a turtle and for the second time from the New World.

Coccidian Parasites (Apicomplexa: Eimeriidae) Of Arkansas Herpetofauna: A Summary With Two New State Records, Chris T. Mcallister, Dagmara Motriuk-Smith, Robert S. Seville, Matthew B. Connior, Stanley E. Trauth, Henry W. Robison

Journal of the Arkansas Academy of Science

Coccidian parasites (Protista: Apicomplexa: Eimeriidae) commonly infect reptiles, and to a lesser degree, amphibians. The family Eimeriidae includes at least 18 genera and 3 of them, Caryospora, Eimeria, and Isospora have been reported previously from various Arkansas herpetofauna. Over the past 3 decades, our community collaborative effort has provided a great deal of information on these parasites found in amphibians and reptiles of Arkansas. Here, we provide a summary of all coccidians reported from herptiles of the state as well as provide 2 new state records for coccidians from non-native Mediterranean geckos, Hemidactylus turcicus.

Exosomes Serve As Novel Modes Of Tick-Borne Flavivirus Transmission From Arthropod To Human Cells And Facilitates Dissemination Of Viral Rna And Proteins To The Vertebrate Neuronal Cells, Wenshuo Zhou, Michael Woodson, Biswas Neupane, Fengwei Bai, Michael B. Sherman, Kyung H. Choi, Girish Neelakanta, Hameeda Sultana

Biological Sciences Faculty Publications

Molecular determinants and mechanisms of arthropod-borne flavivirus transmission to the vertebrate host are poorly understood. In this study, we show for the first time that a cell line from medically important arthropods, such as ticks, secretes extracellular vesicles (EVs) including exosomes that mediate transmission of flavivirus RNA and proteins to the human cells. Our study shows that tick-borne Langat virus (LGTV), a model pathogen closely related to tick-borne encephalitis virus (TBEV), profusely uses arthropod exosomes for transmission of viral RNA and proteins to the human- skin keratinocytes and blood endothelial cells. Cryo-electron microscopy showed the presence of purified arthropod/neuronal exosomes …

An Undergraduate Cell Biology Lab: Western Blotting To Detect Proteins From Drosophila Eye, Neha Gogia, Ankita Sarkar, Amit Singh

Biology Faculty Publications

We have developed an undergraduate laboratory to allow detection and localization of proteins in the compound eye of Drosophila melanogaster, a.k.a fruit fly. This lab was a part of the undergraduate curriculum of the cell biology laboratory course aimed to demonstrate the use of Western Blotting technique to study protein localization in the adult eye of Drosophila. Western blotting, a two-day laboratory exercise, can be used to detect the presence of proteins of interests from total protein isolated from a tissue. The first day involves isolation of proteins from the tissue and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide) gel …

Real-Time Quantitative Pcr To Demonstrate Gene Expression In An Undergraduate Lab, Abijeet Singh Mehta, Amit Singh

Biology Faculty Publications

The objective of this teaching note is to develop a laboratory exercise, which allows students to get a hands-on experience of a molecular biology technique to analyze gene expression. The short duration of the biology laboratory for an undergraduate curriculum is the biggest challenge with the development of new labs. An important part of cell biology or molecular biology undergraduate curriculum is to study gene expression. There are many labs to study gene expression in qualitative manner. The commonly used reporter gene expression studies are primarily qualitative. However, there is no hands-on experience exercise to quantitatively determine gene expression. Therefore, …

Investigating The Relationship Between Instructors’ Use Of Active Learning Strategies And Students’ Conceptual Understanding And Affective Changes In Introductory Biology: A Comparison Of Two Active-Learning Environments, Lacy M. Cleveland, Jeffrey T. Olimpo, Sue Dechenne-Peters

Biology: Faculty Publications

In response to calls for reform in undergraduate biology education, we conducted research examining how varying active-learning strategies impacted students’ conceptual understanding, attitudes, and motivation in two sections of a large-lecture introductory cell and molecular biology course. Using a quasi-experimental design, we collected quantitative data to compare participants’ conceptual understanding, attitudes, and motivation in the biological sciences across two contexts that employed different active-learning strategies and that were facilitated by unique instructors. Students participated in either graphic organizer/worksheet activities or clicker-based case studies. After controlling for demographic and presemester affective differences, we found that students in both active-learning environments displayed …

The Effect Of Arginine Vasotocin On Cryoprotectant Production During Freezing In The Pacific Chorus Frog, Pseudacris Regilla, Ben Ingels

All Master's Theses

Freeze Tolerance is an adaptation seen amongst several species of amphibians as a way of surviving during wintering months. Use of cryoprotectants is seen in all freeze tolerant species (glucose/glycerol). Synthesis and transportation mechanisms that regulate this process are not yet fully understood. Arginine vasotocin is a neurohypophyseal peptide known to have glycogenolytic effects as well as regulate several key processes involved in fluid and solute transportation. Although one prior study suggested that AVT is not involved in responses to freezing, I measured AVT levels during freezing and injected an AVT antagonist to see if it would affect the production …

Thermal And Behavioral Ecology Of The Mexican Beaded Lizard (Heloderma Horridum), Kerry Holcomb

All Master's Theses

Previous investigations in the field of biological thermoregulation have primarily focused on small organisms that inhabit ecosystems that are not as strongly seasonal as the tropical dry forests found along the Pacific Coast of Jalisco, Mexico. Small organisms have little or no appreciable thermal inertia, and thus, reach thermal equilibrium with their immediate surroundings relatively rapidly. In this investigation, we describe the behavioral and thermal ecology of the Mexican beaded lizard (Heloderma horridum), which commonly weighs more than 1kg. This large body size means that this species has significant thermal inertia, which, depending on the characteristics of the …

The Rna Polymerase-Associated Factor 1 Complex Is Required For Plant Touch Responses, Gregory S. Jensen, Kateryna Fal, Olivier Hamant, Elizabeth S. Haswell

Biology Faculty Research

Thigmomorphogenesis is a stereotypical developmental alteration in the plant body plan that can be induced by repeatedly touching plant organs. To unravel how plants sense and record multiple touch stimuli we performed a novel forward genetic screen based on the development of a shorter stem in response to repetitive touch. The touch insensitive (ths1) mutant identified in this screen is defective in some aspects of shoot and root thigmomorphogenesis. The ths1 mutant is an intermediate loss-of-function allele of VERNALIZATION INDEPENDENCE 3 (VIP3), a previously characterized gene whose product is part of the RNA polymerase II-associated factor 1 …

Process-Based Modelling Shows How Climate And Demography Shape Language Diversity, Michael C. Gavin, Thiago F. Rangel, Claire Bowern, Robert K. Colwell, Kathryn R. Kirby, Carlos A. Botero, Michael Dunn, Robert R. Dunn, Joe Mccarter, Marco Tulio Pacheco Coelho, Russell D. Gray

Biology Faculty Research

Aim

Two fundamental questions about human language demand answers: why are so many languages spoken today and why is their geographical distribution so uneven? Although hypotheses have been proposed for centuries, the processes that determine patterns of linguistic and cultural diversity remain poorly understood. Previous studies, which relied on correlative, curve-fitting approaches, have produced contradictory results. Here we present the first application of process-based simulation modelling, derived from macroecology, to examine the distribution of human groups and their languages.

Location

The Australian continent is used as a case study to demonstrate the power of simulation modelling for identifying processes shaping …

Effects Of Wildfires On Rattlesnake (Crotalus Oreganus) Growth And Movement In Washington State, Joseph Chase

All Master's Theses

Fire is a dominant force in the Pacific Northwest that shapes ecosystems and influences wildlife, yet little is known of its effects on local predators. Northern Pacific rattlesnakes (Crotalus oreganus) comprise an excellent model to investigate how fire may influence wildlife because they are important predators that contribute to controlling prey populations, but are also unable to readily escape from wildfires. We developed a novel technique to assess growth rates of rattlesnakes by using digital photography to analyze differences in widths of their rattle segments laid down over time. We compared growth rates of rattlesnakes in habitats that …

First Record Of The Megamouth Shark, Megachasma Pelagios, (Family Megachasmidae) In The Tropical Western North Atlantic Ocean, Grisel Rodriguez-Ferrer, Bradley M. Wetherbee, Michelle Schärer, Craig Lilyestrom, Jan P. Zegarra, Mahmood Shivji

Biology Faculty Articles

Background: A new record of Megachasma pelagios is here reported for the tropical western North Atlantic Ocean from Puerto Rico. Results: On December 10, 2016, a tourist reported an unusual stranded shark on Mojacasabe Beach, Cabo Rojo, on the southwestern coast of Puerto Rico. Visual examination of the carcass and mitochondrial DNA analysis from a dorsal fin sample revealed it to be a 457 cm female megamouth shark. Conclusion: This record represents the first record of M. pelagios for the tropical western North Atlantic Ocean within the Caribbean Sea of southwest Puerto Rico and only the second record of M. …

Burbot Growth Rates In A Northern Mesotrophic Lake, Samuel J. Peterson

Journal of Earth and Life Science

Burbot Lota lota has a circumpolar distribution throughout cold-water areas and is the only freshwater member of the family Gadidae. There are currently very few management techniques applied to Burbot throughout their northern extent. Growth is an indicator of fish health, biomass, and habitat quality; therefore, the objective of this study was to determine the growth rate of Burbot in Lake Bemidji. Burbot (n = 45) were collected along three sites on Lake Bemidji for years 2015-2017. Age was determined from sagittal otoliths, the marginal zones of which were opaque from December to May and translucent from June to November. …

Effects Of Catch-And-Release Angling On Egg Densities In Largemouth Bass Nests, Tyler Robinson

Journal of Earth and Life Science

Predation during early life stages of Centrarchidae offspring are a major cause of decreased reproductive success. For five-weeks Largemouth Bass Micropterus salmoides defend their broods from invaders such as Yellow Perch Perca flavescens and Bluegill Lepomis macrochirus. The focus of this study was to determine if predation upon Largemouth Bass broods increased as removal time from the nest via catch-and-release angling increased. Sampling was conducted on the Whitefish Chain, MN from 7 May - 7 June 2016, where a total of 29 nests were located and sampled. Three treatment groups were used; immediate catch-and-release, three-minute, and a seven- minute …

Effect Of Abiotic Factors On Bluegill Size Structure In Nine Central Minnesota Lakes, Ryan Henry

Journal of Earth and Life Science

Bluegill are the most targeted species of fish in Minnesota. In recent years, the abundance of slow growing, small Bluegill has increased within the state. Angler harvest of Bluegill can reduce the average length of Bluegill up to four times the original length. Abiotic factors such as maximum depth, average water clarity, littoral area, and percent littoral area can influence the dynamics of a lake system. There has been limited research on whether these abiotic factors can influence the size structure of a Bluegill population. The objective of this study is to determine if there is a direct relationship between …

Investigation Of The Anthropogenic Factors Influencing Eastern Fox Squirrel, Sciurus Niger, Distribution And Abundance In Urban Residential Areas, Gabrielle Vinyard

Undergraduate Honors Thesis Collection

In this rapidly developing world, the relationship between humans and wildlife is becoming more strained. Despite the challenges, some animals respond better than others to the additional pressures present in urban environments, and squirrels are a prime example (McKinney, 2002). Several studies have focused on the distribution and abundance of tree squirrels in urban landscapes, but more information is needed to understand the connections between anthropogenic factors and population density (Shochat et al., 2006). Previous research that examined leaf nest densities within residential neighborhoods found a positive correlation between property value and nest density (Salsbury et al., unpublished data). The …

Culture-Independent Analysis Of Microbial Communities Associated With Hydroponically Grown Living Lettuce, Abby Matthews

Honors Theses

The bacteria associated on and within a plant make up that plant's microbiome. Given recent interest in how microbes can affect human health, as well as developments in DNA sequencing that allow us to examine microbial communities without culture techniques, a number of recent studies have investigated the composition of the microbial communities on edible plants. Leafy green salad vegetables pose an added interest, since they are consumed without sterilization or cooking. This study investigated the bacterial composition of endophytic and epiphytic communities of hydroponically grown green leaf lettuce that is sold with the roots intact and referred to as …

Examination Of Electroglottography (Egg) And Surface Electromyography (Semg) As Techniques To Assess Oral Motor Activity During Consumption Of Four Forms Of Chocolate, Taylor Patterson

Honors Theses

The study assessed individual oral motor activity while eating different types of chocolates through the use of electroglottography (EGG) and surface electromyography (sEMG). I hypothesized that chocolate samples with higher milk solids and cocoa composition would be preferred by subjects as assessed by subject evaluation of textural attributes for the four chocolate samples. I predicted that preference will be significantly and positively correlated with oral motor events [e.g. sEMG activity of master and suprahyoid muscles, total number of chewing actions, and time to last swallow (EGG)]. The experiment used a subject group of 40 students enrolled at the University of …

Egg Laying Bird With Male Plumage Demonstrates The Puzzling Nature Of Sexual Differentiation And Selective Mate Preferences In Zebra Finches, John Aaron Howell

Honors Theses

Zebra finches (Taeniopygia guttata) are oscine Passerine songbirds that are native to Australia. Zebra finches have sexually dimorphic plumage, song nuclei, and behavior. The role of chromosomes, hormones, and genes on sexual differentiation are not completely understood. A gynandromorph in our aviary had male plumage, a male partner, and produced viable offspring. Mate preference tests revealed lower preference for the gynandromorph and its progeny than controls, suggesting that they had some traits that made them unattractive to other birds. Gynandromorph lineage males had greater same-sex preferences than control males. Chromosomes in birds are different than those in humans. Males are …

Population Genetics Of The Giant Red Centipede Along The Appalachian Mountains, Kayla Elayne Ladner

Honors Theses

This study focused on population genetics of the giant red centipede, Scolopocryptops sexpinousus, in the Southern Appalachian Mountains. Two genetic markers were used—the mitochondrial COI gene, and the nuclear RNA Polymerase II gene. The goal of this study was to determine how many genetically distinct populations of this invertebrate species exist within the study area, and to understand the spatial distribution of genetic diversity within and among populations. I hypothesized several genetically distinct populations of S. sexpinosus would be detected because the complex topographic characteristics and historical climate cycles of the region are thought to limit movement of many forest …

Bring Your Own Device Initiative To Improve Engagement And Performance In Human Anatomy And Physiology I And Ii Laboratories, Kelsey Hillhouse

Honors Theses

At the University of Mississippi, just 4.93% (2014) and 6% (2015) of Human Anatomy and Physiology students responding to an informal, opinion survey stated that their favorite lab activity was using microscopes. In addition, performance on lab practical questions involving the identification of specimens under a microscope is low with the average percent correct being as low as 31.85% and no higher than 41.94%. These numbers are troubling in that Human Anatomy and Physiology I and II are required courses for students desiring entry into many allied health professions where knowledge of tissues, obtaining samples for biopsy, and interpreting microscopic …