Biology Commons^™

Effects Of Rapid Ph And Salinity Change On The Physiology Of A Local Estuarine Fish Species, Fundulus Heteroclitus, Shauna Tietze

GS4 Student Scholars Symposium

Coastal Georgia offers a wide variety of unique aquatic environments, one of the most variable of these environments are salt marshes. Sapelo Island, Georgia, is a pristine environment that is home to many variable salt marshes. Throughout marshes, salinity ranges from freshwater to full strength sea water and acidity levels range from neutral (pH 7) to low pH (pH < 4.5). Fundulus heteroclitus (Atlantic killifish) is an example of an organism that has evolved coping strategies to withstand such a variable environment. Many laboratory studies have shown that F. heteroclitus exhibit a broad tolerance range of single stressors, however few have studied their ability to handle multiple stressors simultaneously. Our goal is to determine if exposure to low salinity and low pH combined is more stressful to F. heteroclitus than individual exposure to low salinity or low pH. To address this area of interest a laboratory experiment was designed. F. heteroclitus were captured from a site with neutral pH (6.8) and mid-range salinity (16 ppt) and held in the laboratory for one month at the same water conditions. The water conditions of the capture site represents similar conditions to where the fish were most commonly found on the island. Fish were then placed in tanks with four different water treatments; low salinity, low pH, low salinity and low pH combined, and a control and held at these conditions for one week. Six fish were sampled from each treatment group at 24 hours, 5 days, and 7 days post transfer. During sampling fish were euthanized, the gills were taken for gene expression analysis and the rest of the body was used for cortisol analysis. Changes in the mRNA expression levels of Cystic Fibrosis Transmembrane Conductance Regulator and Na /K -ATPase were compared between treatments using qRT-PCR analysis. These genes have been shown to play an important role in ion regulation in the gills by altering cell volume and ion movement in the sodium/potassium pump. Cortisol was also analyzed using an ELISA kit (Neogen) as a general stress response measure. I hypothesize that results from gene expression and cortisol analysis will show that F. heteroclitus challenged with low salinity and low pH combined experience a higher level of stress compared to fish challenged with only low salinity or low pH, exhibited by an increase in cortisol levels and increased expression of genes responsible for ion balance The information gained from this project will provide a better understanding of the stress this species exhibits due to rapid pH and salinity change. As well as, whether fish can rapidly adapt to changing conditions. Given the ubiquity of the common killifish and its importance in estuarine food webs, these data may provide insight into how we can manage and maintain diverse fish populations and support ecosystem functioning.

Detection Of Wolbachia In Human Lice, Matthew Anderson

GS4 Student Scholars Symposium

Background: Candidatus Riesia pediculicola is the primary endosymbiont of human lice which is important to the louse survival. However, bioinformatic analysis of the Riesia genome revealed that its vitamin B1 pathways are not complete in Riesia. These findings suggest that a secondary louse endosymbiont may be present and responsible for the missing functions. . Wolbachia, an obligate intracellular alpha proteobacteria, is a candidate for this role, similar to secondary symbionts in other arthropod hosts including those for tsetse flies, aphids and many other insects with deficient diets. The purpose of this study is to determine the infection rate of Wolbachia …

Isolation And Characterization Of Six Novel Rhodobacter Capsulatus Bacteriophages, Addison Ely, Alexandria Paradis, Brook Koebele, Richard Alvey, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

Functional Analysis Of A Putative Homing Endonuclease, Nikhilesh Thapa, David Bollivar, Faculty Advisor

John Wesley Powell Student Research Conference

Endonucleases are enzymes that cleave phosphodiester bonds within polynucleotide chains such as DNA and RNA. A subgroup, homing endonucleases (HEase) are also able to propagate their encoding genes by transforming host genes to incorporate the homing endonuclease gene (HEG). This process is initiated by the expression of the HEase from the HEG, which then cleaves a homolog. The homolog subsequently undergoes a homologous recombination event as a repair mechanism, and in the process integrates a copy of the HEG. Based on predicted sequence, gene product (gp126) from the mycobacteriophage Gizmo is thought to encode for a HEase. Previous work constructed …

Genome Annotation Of 3 New Rhodobacter Capsulatus Bacteriophages, Seth Borrowman, Niyant Vora, Emily Erdmann, Madeline Gibson, Richard Alvey, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

Assessment Of Cranial Neural Crest Proliferation Patterns Between The Redeye Tetra Moenkhausia Sanctaefilomenae And The Zebrafish Danio Rerio, Lesly Ingram, Brian Walter, Faculty Advisor

John Wesley Powell Student Research Conference

Among teleost fishes, there is a substantial amount of diversity regarding craniofacial morphology. This study investigated the cellular processes directing the morphological variations observed in the Meckel’s and ceratohyal cartilages of the zebrafish Danio rerio and the redeye tetra Moenkhausia sanctaefilomenae. Utilizing BrdU incorporation, significant interspecific variations relating to cartilage formation were determined. Specifically, interstitial proliferation was found to be more important for the shaping and subsequent growth of the cartilages in D. rerio than in M. sanctaefilomenae. Correspondingly, the expansion of the pharyngeal arches was more dramatic in M. sanctaefilomenae than in D. rerio. This study demonstrates …

A Transposon Mutagenesis Screen For Heterocyst Production In A ∆Hetp Strain Of The Cyanobacterium Anabaena, Rachel Ende, Loralyn Cozy, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

Determining The Viability Of Preserved Cell Suspensions Frozen Over Time With Varied Glycerol Concentrations, Elyse Mccormick, Brian Walter, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

A New Technique For Imaging Real-Time Cytokine Secretion, Sydney Muchnik, Brian Walter, Faculty Advisor, Ramesh Ramji, Faculty Advisor, Kathryn Miller-Jensen, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

Restoration Of Heterocyst Production To A ∆Hetp Strain Of Anabaena, Megan Smeets, Loralyn Cozy, Faculty Advisor

John Wesley Powell Student Research Conference

Poster presentation abstract.

Design Science 5 | Steam Intelligence, Schedule, Edna W. Lawrence Nature Lab

Design Science Symposia

Schedule of events.

Sex Ratio And Gamete Size Across Eastern North America In Dictyostelium Discoideum, A Social Amoeba With Three Sexes, Tracy Edwards Douglas, Joan E. Strassmann, David C. Queller

Biology Faculty Research

Theory indicates that numbers of mating types should tend towards infinity or remain at two. The social amoeba, Dictyostelium discoideum, however, has three mating types. It is therefore a mystery how this species has broken the threshold of two mating types, but has not increased towards a much higher number. Frequency-dependent selection on rare types in combination with isogamy, a form of reproduction involving gametes similar in size, could explain the evolution of multiple mating types in this system. Other factors, such as drift, may be preventing the evolution of more than three. We first looked for evidence of …

Parental Influences On The Behavior Of A Juvenile Bornean Orangutan (Pongo Pygmaeus), Raechel Mcumber, Sarah Robarts, Gregory B. Cunningham

The Review: A Journal of Undergraduate Student Research

Here we discuss the influence of the mother and father on the behaviors of a juvenile Bornean Orangutan (Pongo pygmaeus). This species is known for being solitary and asocial, in that males and females do not live together, while living in their natural environment. However, adults are usually housed together in zoo settings. We observed a juvenile and both of her parents in their zoo enclosure and recorded the play and parentally influenced behaviors, such as swinging and eating, performed by the juvenile. It was found that the juvenile played and performed parentally influenced behaviors significantly more while in …

Linking Native And Invader Traits Explains Native Spider Population Responses To Plant Invasion, Jennifer N. Smith, Douglas J. Emlen, Dean Pearson

Biological Sciences Faculty Publications

Theoretically, the functional traits of native species should determine how natives respond to invader-driven changes. To explore this idea, we simulated a large-scale plant invasion using dead spotted knapweed (Centaurea stoebe) stems to determine if native spiders’ web-building behaviors could explain differences in spider population responses to structural changes arising from C. stoebe invasion. After two years, irregular web-spiders were >30 times more abundant and orb weavers were >23 times more abundant on simulated invasion plots compared to controls. Additionally, irregular web-spiders on simulated invasion plots built webs that were 4.4 times larger and 5.0 times more likely …

Bats Of The Monongahela National Forest, Jason D. Collins

Master's Theses and Doctoral Dissertations

Populations of bats across the eastern United States have experienced significant declines in recent years. Insight into their distributions and habitat associations, as well as understanding population trends, will aid in conservation efforts. In this study, the geographic distributions of the 10 species of bats found in the Monongahela National Forest of West Virginia were analyzed, using maximum entropy modeling and mist-netting data from 1997 through 2012. Forest type, hydrologic features, elevation, land-cover type, and distance to caves were typically the most important explanatory variables within these models, although results varied among species. By understanding these foraging habitat associations and …

Caenorhabditis Elegans Paraoxonase-Like Proteins Control The Functional Expression Of Deg/Enac Mechanosensory Proteins, Yushu Chen, Shashank Bharill, Zeynep Altun, Robert O'Hagan, Brian Coblitz, Ehud Y. Isacoff, Martin Chalfie

Department of Biology Faculty Scholarship and Creative Works

Caenorhabditis elegans senses gentle touch via a mechanotransduction channel formed from the DEG/ENaC proteins MEC-4 and MEC-10. An additional protein, the paraoxonase-like protein MEC-6, is essential for transduction, and previous work suggested that MEC-6 was part of the transduction complex. We found that MEC-6 and a similar protein, POML-1, reside primarily in the endoplasmic reticulum and do not colocalize with MEC-4 on the plasma membrane in vivo. As with MEC-6, POML-1 is needed for touch sensitivity, the neurodegeneration caused by the mec-4(d) mutation, and the expression and distribution of MEC-4 in vivo. Both proteins are likely needed for the proper …

Discriminative Stimulus Properties Of 3.0 Mg/Kg Mephedrone In Rats, Nathyn Thompson

Honors Theses

Consumption of a prominent synthetic cathinone known as mephedrone (4-methylmethcathinone) has become a popular alternative to club drugs such as ecstasy, cocaine, and methamphetamine within the past decade. The pharmacological mechanisms that contribute to its subjective effects have yet to be fully characterized and are thus warranted for investigation. The present study employed drug discrimination methods to train eight male Sprague-Dawley rats to discriminate injections of 3.0 mg/kg mephedrone from saline. Various doses of mephedrone, 3,4-methylenedioxypyrovalerone (MDPV), d-amphetamine (AMPH), cocaine, (+)-methamphetamine (METH), and 3,4-methylenedioxy-methamphetamine (MDMA) were investigated for their ability to substitute for the stimulus cues of the training …

Apical Testis Structure And The Effects Of Cadmium Treatment On Spermatogenesis In Drosophila, Paulina J. Cardaci

Seton Hall University Dissertations and Theses (ETDs)

The fruit fly Drosophila melanogaster is used extensively as a model for studying molecular, genetic and cellular aspects of human disease and physiology. Our lab has used D. melanogaster and related species to study the structure of the testis stem cell niche, as well as other aspects of spermatogenesis. We previously revealed a novel stem cell niche structure in D. pseudoobscura, a distant relative of D. melanogaster. The signaling center of the D. melanogaster stem cell niche has a well-characterized rosette arrangement of fasciclin-positive cells terms the “hub”. D. pseudoobscura, however, lacks a punctuate hub and instead displays a …

Variation In Stem Mortality Rates Determines Patterns Of Above-Ground Biomass In Amazonian Forests: Implications For Dynamic Global Vegetation Models, Michelle O. Johnson, David R. Galbraith, Manuel Gloor, Hannes De Deurwaerder, Matthieu Guimberteau, Anja Rammig, Kirsten Thonicke, Hans Verbeeck, Celso Von Randow, Bradley O. Christoffersen

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Understanding the processes that determine above-ground biomass (AGB) in Amazonian forests is important for predicting the sensitivity of these ecosystems to environmental change and for designing and evaluating dynamic global vegetation models (DGVMs). AGB is determined by inputs from woody productivity [woody net primary productivity (NPP)] and the rate at which carbon is lost through tree mortality. Here, we test whether two direct metrics of tree mortality (the absolute rate of woody biomass loss and the rate of stem mortality) and/or woody NPP, control variation in AGB among 167 plots in intact forest across Amazonia. We then compare these relationships …

Movies With Meaning: Crossing Disciplines In Cinema, Ashley Cotter

Scholarly and Creative Works Conference (2015 - 2021)

One might not realize that blockbuster movies can be used as an educational tool in the classroom setting to enhance the learning process. While these types of movies are not crafted as pedagogical tools, like animated, demonstrative video clips, there is educational value to be found in them. In more recent years, this is a practice that has been used in many classrooms. However, it is within the social sciences primarily that these blockbuster films are used to augment the understanding of concepts. Big films are rarely, if ever, used in the hard sciences to provide examples of concepts. How …

Presence Of Wolbachia In Sandfly Populations And Correlation With Pesticide Resistance, Cody Bills, Carol Von Dohlen, Katie Weglarz

Student Research Symposium

Leishmaniasis is a serious skin disease, affecting 12 million people worldwide, caused by a protozoan vectored by sand flies. These flies can harbor the bacterium Wolbachia, a microorganism which blurs the line between symbiont and parasite. On one hand, it skews sex ratios in sand fly populations but, on the other hand, it confers certain benefits, specifically increased insecticide resistance. In closely related flies, specifically the mosquito, pesticide resistance is developed when Wolbachia infection rates increase. The range of impacts of Wolbachia on their hosts may offer a secondary control pathway for sand fly populations where pesticide resistance has developed, …

Development Of Archaeal And Algalytic Bacteria Detection Systems, Andrew Walters

Student Research Symposium

Many methods for the production of methane are being developed in response to the growing energy crisis. One such method is the digestion of wastewater algae in a UASB (Up-Flow Anaerobic Sludge Blanket) reactor. In this reactor, algae grown in wastewater treatment facilities can be digested by alga-lytic bacteria into its carbon rich substituents and later used by methanogenic archaebacteria to produce methane.

The objective of this project is to design detection systems that employ specific DNA primers that will precisely target two bacterial groups: alga-lytic and archaeal bacteria. If successful, these systems will allow us to identify the bacteria …

Tag-Team Takeover: Usurpation Of Woodpecker Nests By Western Bluebirds, Sammy Cowell, Hannah Domgaard, Phil Fischer, Teresa Lorenz, Sara Lorscheider, Mariah Panoussi, Lindsey Parrish, Taryn Rodman, Kim Sullivan

Student Research Symposium

Woodpeckers provide important ecological services by excavating nesting cavities that are used by many forest birds and animals. Black-backed woodpeckers (Picoides arcticus) have been declining throughout their range and are now a threatened species. The failure of black-backed woodpeckers to reproduce can lead to a decline of nesting cavities, which in turn can lead to a decline in secondary cavity nesters. One of the challenges black-backed woodpeckers face is displacement from their nesting cavities by aggressive secondary cavity nesters.The western bluebird (Sialia Mexicana) is known to usurp nests, even from its own kind. However, the process by which bluebirds take …

Using Koch’S Postulate To Identify The Causative Agent Of Dieback In Malosma Laurina During Historic Drought In California, Adriana J. Borges, Emily R. Pierce, Mariah K. Taylor, Frida J. Morales

Featured Research

Coastal Southern California has a Mediterranean-type climate, meaning that it experiences warm, dry summers and cool, wet winters. Recently, California has been experiencing an unprecedented drought, possibly as a result of climate change. Malosma laurina is a typically hearty chaparral plant is known for being resistant to drought due to its deep rooting depth, but even it is being negatively affected by drought and is experiencing dieback. Some of this dieback is caused by a Botryospharae fungal infection, which blocks xylem conduits and stops water flow through the plant. With a looming El Niño, this study seeks to understand the …

Why Are Students Doing Research? Examining The Motivation Of Students Involved In Undergraduate Research Programs, Andrew Layne Mcdevitt

Theses and Dissertations

Use of self-determination theory (SDT) within the science classroom focuses primarily on ways to integrate intrinsic motivation into students' identity. Experiential learning plays a large role in promoting learning by shaping students' interests, identity, and intrinsic aspirations. This phenomenological research study sought to understand how experiential learning experiences helped influence career aspirations of graduate students within ecological disciplines. By determining how their experiences met three basic psychological needs outlined by SDT (competence, autonomy, and relatedness), we were able to examine which regulators drove motivation.

In the qualitative pilot study, participants developed a genuine enjoyment and appreciation for their discipline as …

Influence Of Water Hardness On Accumulation And Effects Of Silver In The Green Alga, Raphidocelis Subcapitata, Aarya Venkat, Adriana L. Amerson, Gretchen K. Bielmyer-Fraser

Georgia Journal of Science

Metal pollution from anthropogenic sources can pose a threat to aquatic systems. Silver is released into the environment from various industrial processes. In excess, silver can accumulate and cause adverse effects in aquatic organisms, particularly those in lower trophic levels, such as phytoplankton. Water chemistry parameters, such as hardness, have been shown to modify toxicity of metals because divalent cations compete with the metal for binding sites on the biological membrane. The objective of this study was to assess population growth and silver accumulation in the green alga, Raphidocelis subcapitata, after silver exposure in waters of varying hardness for 7 …

Department Of Biology And Earth Science: 2016 Senior Symposium, Otterbein University

Biology and Earth Science Student Scholarship

No abstract provided.

Factors Affecting The Immune System Of The Ornate Box Turtle (Errapene Ornata), Michelle W. Mcquinn, Abigail A. Neyer, Gwendolyn C. Bachman

UCARE: Research Products

While many studies have detailed the complex intricacies of the endothermic immune system, relatively little is known about the immune system of ectotherms--specifically, reptiles. In an attempt to gain more knowledge about the factors affecting reptilian immune function, ornate box turtles (Terrapene ornata) were subjected to chronic stress in the form of high ambient temperatures in a semi-natural environment. It was hypothesized that chronic stress would lead to elevated levels of corticosterone in the blood, which would, in turn, suppress immune activity. It was found that body temperature and body mass in particular were significantly affected by chronic heat stress. …

Consensus On Consensus: A Synthesis Of Consensus Estimates On Human-Caused Global Warming, John Cook, Naomi Oreskes, Peter T. Doran, William R L Anderegg, Bart Verheggen, Ed W. Maibach, J Stuart Carlton, Stephan Lewandowsky, Andrew G. Skuce, Sarah A. Green, Dana Nuccitelli, Peter Jacobs, Mark Richardson, Bärbel Winkler, Rob Painting, Ken Rice

Department of Chemistry Publications

The consensus that humans are causing recent global warming is shared by 90%–100% of publishing climate scientists according to six independent studies by co-authors of this paper. Those results are consistent with the 97% consensus reported by Cook et al(Environ. Res. Lett. 8 024024) based on 11 944 abstracts of research papers, of which 4014 took a position on the cause of recent global warming. A survey of authors of those papers (N = 2412 papers) also supported a 97% consensus. Tol (2016 Environ. Res. Lett.11 048001) comes to a different conclusion using …

Implications Of Notch Signaling Pathway In Breast Cancer, Anuja D. Shimpale

Research Horizons Day Posters

No abstract provided.