Biology Commons^™

Antimicrobial And Antiinsectan Phenolic Metabolites Of Dalea Searlsiae, Gil Belofsky, Mario Aronica, Eric Foss, Jane Diamond, Felipe Santana, Jacob Darley, Patrick F. Dowd, Christina M. Coleman, Daneel Ferreira

All Faculty Scholarship for the College of the Sciences

Continued interest in the chemistry of Dalea spp. led to investigation of Dalea searlsiae, a plant native to areas of the western United States. Methanol extractions of D. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans A–D (1–4) and known flavanones (5 and 6). Known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. Structure determination of pure compounds was accomplished primarily by extensive 1D- and 2D-NMR spectroscopy. The absolute configurations of compounds 1–5, 7 …

The Occurrence And Consequences Of Conspecific Brood Parasitism In The Prothonotary Warbler (Protonotaria Citrea), Anna Tucker

Theses and Dissertations

Brood parasites avoid costs associated with raising young by adding eggs to another individual’s clutch and providing no parental care. When conspecific brood parasitism occurs in species with high parental investment, we expect hosts to suffer costs for raising an enlarged brood. Here I describe conspecific brood parasitism (CBP) in the prothonotary warbler using maternal exclusion analyses of 333 family groups. I found that 23.4% of clutches contained at least one offspring that was not matched to the social mother and determined that parasitism seems to be an opportunistic tactic. Hosts had lower average annual reproductive success than non-hosts, but …

Implications Of Climate Change On The Distribution Of The Tick Vector Ixodes Scapularis And Risk For Lyme Disease In The Texas-Mexico Transboundary Region, Teresa Patricia Feria-Arroyo, Ivan Castro-Arellano, Guadalupe Gordillo Pérez, Ana L. Cavazos, Margarita Vargas-Sandoval, Abha Grover, Javier Torres, Raul F. Medina, Adalberto A. Pérez De León, Maria Dolores Esteve-Gassent

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Background: Disease risk maps are important tools that help ascertain the likelihood of exposure to specific infectious agents. Understanding how climate change may affect the suitability of habitats for ticks will improve the accuracy of risk maps of tick-borne pathogen transmission in humans and domestic animal populations. Lyme disease (LD) is the most prevalent arthropod borne disease in the US and Europe. The bacterium Borrelia burgdorferi causes LD and it is transmitted to humans and other mammalian hosts through the bite of infected Ixodes ticks. LD risk maps in the transboundary region between the U.S. and Mexico are lacking. Moreover, …

Sink Or Ecological Trap For Tree Swallows In Central Minnesota?, James T. Pathoulas, Nicole E. Noyes

Celebrating Scholarship & Creativity Day (2011-2017)

Background: An ecological trap is typically defined as a low-quality habitat, incapable of sustaining a population, which is preferred over a high quality habitat. Ecological traps may lead to species extinction in populations with 1) strict habitat requirements 2) minimal information about the habitat, and 3) low population size. Tree Swallows (Tachycineta bicolor) meet the former two of these criteria.

Methods: We monitored Tree Swallow (TS) nest boxes (n=90) at two ecologically similar sites A and B with similar occupancy rates. Site B has lower reproductive fitness —suggestive of an ecological trap. Binary logistic regression was used to …

Development Of A System For Analysis Of Muscle Contraction Pattern During Drosophila Melanogaster Crawling Behavior, Mary Catherine Decker

Celebrating Scholarship & Creativity Day (2011-2017)

The aim of this study was to attain footage of the crawling Drosophila larva from 360° angles in order to visualize the pattern of individual muscle contractions. A GAL4-UAS system was used to drive homozygous GCAMP expression, causing the muscles of the Drosophila larva to fluoresce when contracting. A stand was designed in order to place a live, crawling larva in a glass capillary tube under a fluorescent microscope and then rotate the tube completely for 360° video footage. Through the combination of the GCAMP expression and the rotatable stand, video of the individual muscle contraction pattern of a crawling …

Vegetation And Climate History Of The St. John’S Abbey Arboretum (Collegeville, Minnesota) Based Upon Lacustrine Fossil Pollen, Emily Kiolbasa

Celebrating Scholarship & Creativity Day (2011-2017)

No abstract provided.

Chronic Lymphocytic Leukemia Cells In A Lymph Node Microenvironment Depict Molecular Signature Associated With An Aggressive Disease, Amit K. Mittal, Nagendra K. Chaturvedi, Karan J. Rai, Christine E. Gilling-Cutucache, Tara M. Nordgren, Margaret Moragues, Runqing Lu, Rene Opavsky, Greg R. Bociek, Dennis D. Weisenburger, Javeed Iqbal, Shantaram S. Joshi

Biology Faculty Publications

Chronic lymphocytic leukemia (CLL) cells survive longer in vivo than in vitro, suggesting that the tissue microenvironment provides prosurvival signals to tumor cells. Primary and secondary lymphoid tissues are involved in the pathogenesis of CLL, and the role of these tissue microenvironments has not been explored completely. To elucidate host-tumor interactions, we performed gene expression profiling (GEP) of purified CLL cells from peripheral blood (PB; n = 20), bone marrow (BM; n = 18), and lymph node (LN; n = 15) and validated key pathway genes by real-time polymerase chain reaction, immunohistochemistry and/or TCL1 trans-genic mice. Gene signatures representing several …

Forest Edges Enhance Mate-Finding In The European Gypsy Moth, Lymantria Dispar, Lily Thompson

Theses and Dissertations

Understanding movement capabilities of individuals within a landscape is essential to identifying the effects of habitat boundaries on species abundances, ranges, and spread rates. Movement barriers due to habitat fragmentation may reduce mate-finding ability in some species, particularly in heterogeneous landscapes containing low-density populations. This study focuses on the effects of habitat type and edge on mate-finding in an invasive defoliator, the European gypsy moth. Adult European gypsy moth males locate mates by following pheromones released by flightless females. Reduced mate-finding was expected in fields and near forest edges based on geographic variation in invasion rates and pheromone plume dynamics. …

Association Of Rare Genetic Variants To The Development And Progression Of Alzheimer’S Disease, Cameron Schmutz, Dr. Keoni Kauwe

Journal of Undergraduate Research

Alzheimer’s disease (AD) is a fatal neurodegenerative disorder and is the leading cause of dementia.1,3 It is the sixth leading cause of death in the United States and is the only one that has no adequate prevention or cure. AD is characterized by a progressive loss in cognitive function and memory loss. Neuropathological symptoms include loss of neurons and synapses, extensive accumulation of amyloid plaques and neurofibrillary tangles. Alzheimer’s disease has been identified as a proteopathy disease due to the extensive accumulation of amyloid plaques and neurofibrillary tangles.1,2,3 This project focused on the research of a rare genetic variant in …

Evolution Of Ectoparastism In Earwigs (Insect: Dermaptera), Michael Naegle, Dr. Michael Whiting

Journal of Undergraduate Research

Dermaptera (earwigs) is a small order of insects, typically characterized by the presence of cerci on the abdomen that are modified into forceps-like appendages (Giles, 1963). Dermaptera includes approximately 2200 extant species comprising 11 families (Kocarek 2013). The majority of earwigs are free-living feeding on detritus and other plant material (Sakai 1987). Two families, Arixeniidae and Hemimeridae are ectoparasitic, associating with Malay Peninsula bats and giant murid rats of sub- Saharan Africa respectively (Jarvis 2005).

Population-Based Analysis Of Cetp Identifies Association Between I405v And Cognitive Decline: The Cache County Study, Caitlin Munger, Dr. John Kauwe

Journal of Undergraduate Research

Late-onset Alzheimer’s disease (LOAD) is a fatal neurodegenerative disease and is the sixth leading cause of death in the US. There are currently no successful therapies to prevent or treat LOAD, and predicting disease status remains a challenge1. Apolipoprotein E (APOE)—a gene involved in cholesterol regulation—is the strongest genetic predictor for LOAD. Because of the association between APOE and LOAD, cholesterol levels were suspected and have since been shown to increase LOAD risk2. Similar to APOE, the Cholesteryl Ester Transfer Protein (CETP) gene facilitates cholesteryl ester and triglyceride exchange between lipoproteins3 and is found in the same pathway as APOE, …

Heteroplasmic Mitochondria Project, Lyndsay Staley

Journal of Undergraduate Research

Unfortunately, my Heteroplasmic Mitochondria project was picked up by a commercial entity whom we had asked for the sequenced genomes, but they beat me to the answer before releasing the sequenced genomes. I worked on another project involving Alzheimer’s disease genetics. I was able to do the initial analyses in a genome-wide association study of cerebrospinal fluid levels of 59 Alzheimer’s Diseaserelated analytes from the Rules Based Medicine panel in two independently collected and measured series (ADNI and Knight ADRC). Now I am working with these results to further our knowledge of the genes that cause AD. We will be …

Genetic Variants In The Apoe Gene Region Responsible For Change In Cerebrospinal Fluid Ab42 Protein Levels, Spencer Foutz, Dr. John Kauwe

Journal of Undergraduate Research

For the last several years, the e4 allele in the APOE gene region has been a strong determinant of the development of Amyloid Beta plaques in the brain’s cerebral spinal fluid. As these plaques aggregate, the deposits become increasingly deadly to neuronal cells, and can eventually lead to Alzheimers’ Disease. While the e4 allele is a strong indicator of change in Amyloid Beta levels, there are believed to be other genetic variants within the APOE gene region that also greatly influence this protein. Our purpose was to further evaluate previous studies on this gene region in order to determine other …

Robust And Versatile Bioinformatic Pipeline Development, Ryan Hillary, Dr. Seth Bybee

Journal of Undergraduate Research

With the many advances in sequencing technology and exponential growth of computational capacities there comes a huge demand for processing larger and larger quantities of data. With respect to Biology there can be hundreds of gigabytes of data that can be involved in a single research study that requires computational analysis. Since each research project is unique in terms of design and desired outcome, the programs and tools that are required can vary greatly, but there are some analyses that are similar and can be compiled and optimized. The current research being conducted by Dr. Bybee focuses on the visual …

Biodiversity And Distribution Of Entomophilic Nematodes In Utah’S Mojave Region, Westen Archibald, Dr. Byron Adams

Journal of Undergraduate Research

To better understand the diversity and distribution of entomophilic nematodes in Utah’s Mojave region, I have begun a project that’s purpose is to develop phylogenetic maps of 55 nematode species that I have already extracted from the soils of the Beaver Dam wash ecotone. This project involves both the reconstruction of evolutionary relationships to recover biodiversity, and generating a species distribution map for the Beaver Dam wash ecotone.

Immunogenicity Of A West Nile Virus Diii-Cholera Toxin A2/B Chimera After Intranasal Delivery, Juliette K. Tinker, Jie Yan, Reece J. Knippel, Panos Panayiotou, Kenneth A. Cornell

Biology Faculty Publications and Presentations

West Nile virus (WNV) causes potentially fatal neuroinvasive disease and persists at endemic levels in many parts of the world. Despite advances in our understanding of WNV pathogenesis, there remains a significant need for a human vaccine. The domain III (DIII) region of the WNV envelope protein contains epitopes that are the target of neutralizing antibodies. We have constructed a chimeric fusion of the non-toxic cholera toxin (CT) CTA₂/B domains to DIII for investigation as a novel mucosally-delivered WNV vaccine. Purification and assembly of the chimera, as well as receptor-binding and antigen delivery, were verified by western blot, …

The Effect Of Regional And Seasonal Variability In Wastewater Microbial Community Structure On The Biodegradability Of Room Temperature Ionic Liquids, Steven Aiello

Honors Theses

Biodegradability is an important consideration in the design of novel chemicals. At the end of their function, chemicals should break down into harmless degradation products to avoid toxic effects as a result of environmental persistence. To test the biodegradability potential of novel chemicals, a protocol designed by the Organisation for Economic and Cooperative Development (OECD) is used. This protocol states that in a mixture of the chemical of interest, a microbial inoculate, and mineral media, 70% of the chemical must degrade within a 10-day window of a 28-day experiment in order to be considered readily biodegradable. The protocol states that …

Assessment Of Trem2 Rs75932628 Association With Alzheimer’S Disease In A Population-Based Sample: The Cache County Study, Josue D. Gonzalez Murcia, Cameron Schmutz, Caitlin Munger, Ammon Perkes, Aaron Gustin, Michael Peterson, Mark T. W. Ebbert, Maria C. Norton, Joann T. Tschanz, Ronald G. Munger, Christopher D. Corcoran, John S. K. Kauwe

Journal of Undergraduate Research

Recent studies have identified the rs75932628 (R47H) variant in TREM2 as an Alzheimer’s disease risk factor with estimated odds ratio ranging from 2.9 to 5.1.

Biological Relevance Of Cnv Calling Methods Using Familial Relatedness Including Monozygotic Twins, Christina A. Castellani, Shiva M. Singh, Elizabeth O. Locke

Biology Publications

Studies involving the analysis of structural variation including Copy Number Variation (CNV) have recently exploded in the literature. Furthermore, CNVs have been associated with a number of complex diseases and neurodevelopmental disorders. Common methods for CNV detection use SNP, CNV, or CGH arrays, where the signal intensities of consecutive probes are used to define the number of copies associated with a given genomic region. These practices pose a number of challenges that interfere with the ability of available methods to accurately call CNVs. It has, therefore, become necessary to develop experimental protocols to test the reliability of CNV calling methods …

Temporal And Spatial Variation Of Nitrogen In Wyoming Big Sagebrush, Stephanie Williams, Jordan Nobler, Kristina Gehlken, Janet Rachlow, Lisa Shipley

College of Arts and Sciences Presentations

Sagebrush steppe systems represent one of North America’s greatest conservation challenges. Shrinking habitat and declining animal populations have prompted researchers to fill gaps in our understanding of the ecology of this system, particularly at a scale relevant to individual animals. What animals eat and why are fundamental questions linked to habitat quality and use. We investigated the temporal and spatial heterogeneity of the dietary quality of food to better understand habitat use by a sagebrush specialist, the pygmy rabbit (Brachylagus idahoensis). Within a foraging patch, pygmy rabbits selectively browse on individual Wyoming big sagebrush (Artemisia tridentata wyomingensis) that are …

Students Name Jaeckle Professor Of The Year, Hannah Dhue

News and Events (Discontinued Series)

No abstract provided.

Fifth Specimen Of The Nine-Banded Armadillo (Dasypus Novemcinctus) From Nebraska, Keith Geluso, Greg D. Wright, Angelina E. Wright

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The nine-banded armadillo (Dasypus novemcinctus) has moved northward in the Great Plains in the last century, reaching distributional limits in Nebraska for pioneering individuals. Herein, we report on the fifth specimen from Nebraska. A male individual was obtained from Lincoln County representing a new county record for Nebraska and one of the northwestern-most specimens in the United States.

Using Deuterium And Garp To Estimate Geographic Extents Of Source Populations Of Hoary ( Lasiurus Cinereus ) And Eastern Red ( Lasiurus Borealis ) Bats Killed At A Central Illinois Wind Facility, Rachael Anne Van Essen

Theses and Dissertations

High bat mortality at wind energy facilities is a widely cited conservation issue, but the population-level impacts are not understood. In Illinois, the main species affected are migratory tree bats like the Hoary (Lasiurus cinereus) and Eastern Red (Lasiurus borealis).This research used deuterium isotope analysis of hair combined with ecological niche modeling (GARP: Genetic Algorithm for Rule-set Prediction) in a novel way to map the geographic extents of Hoary and Eastern Red bat specimens salvaged at a single central Illinois wind facility from 2008-2010. Hair was chosen after determining that the claw is currently a problematic tissue due to a …

Walk-Training Increases Expression Of Gdnf In Pectoralis Muscle But Not Diaphragm From Mouse, Erin Donovan

Honors Theses

Neurotrophic factors are proteins that play an important role in the development and maintenance of neurons. Recent studies have shown that neurotrophic factors may hold promise for treating damage to the nervous system caused by trauma or diseases, such as amyotrophic lateral sclerosis or Parkinson’s disease. Glial cell line-derived neurotrophic factor (GDNF) is expressed in skeletal muscles and affects peripheral motor neurons. Results of previous studies have demonstrated that exercise can increase GDNF content in skeletal muscle of rat. The goal of the current study was to examine whether expression of GDNF in skeletal muscle of mouse is also regulated …

Overview Of The Integrated Pest Management (Ipm) Terrain And Activities In Furtherance Of The Walmart Initiative To Purchase Fresh Vegetables From Local Growers In Alabama, Franklin Quarcoo, Conrad Bonsi

Professional Agricultural Workers Journal

Tuskegee University has been providing various types of technical expertise to limited resource farmers who have been supplying Walmart with collard greens, watermelons and purple hull peas. A number of pests bedevil the cultivation of these crops; cost-effective management methods for these pests are needed. The objectives of this paper are to document the IPM activities associated with supplying produce to Walmart; summarize pest problems encountered on the afore-stated crops; recommend IPM methods suitable for limited resource farmers; and suggest other activities that ensure that farmers incur even less pest-related crop losses. Anthracnose was the major pest encountered on watermelons. …

Single Nucleotide Polymorphism Discovery In Cutthroat Trout Subspecies Using Genome Reduction, Barcoding, And 454 Pyro-Sequencing, Derek D. Houston, David B. Elzinga, Peter J. Maughan, Scott M. Smith, John S. K. Kauwe, R. Paul Evans, Ryan B. Stinger, Dennis K. Shiozawa

Journal of Undergraduate Research

Salmonids are popular sport fishes, and as such have been subjected to widespread stocking throughout western North America. Historically, stocking was done with little regard for genetic variation among populations and has resulted in genetic mixing among species and subspecies in many areas, thus putting the genetic integrity of native salmonid populations at risk and creating a need to assess the genetic constitution of native salmonid populations. Cutthroat trout is a salmonid species with pronounced geographic structure (there are 10 extant subspecies) and a recent history of hybridization with introduced rainbow trout in many populations. Genetic admixture has also occurred …

Variation In Arabidopsis Flowering Time Associated With Cis-Regulatory Variation In Constans, Ulises Rosas, Yu Mei, Qiguang Xie, Joshua A. Banta, Royce W. Zhou, Gabriela Seufferheld, Silvia Gerard, Lucy Chou, Naeha Bhambhra, Jennifer Deane Parks, Jonathon M. Flowers, C. Robertson Mcclung, Yoshie Hanzawa, Michael D. Purugganan

Biology Faculty Publications and Presentations

The onset of flowering, the change from vegetative to reproductive development, is a major life history transition in flowering plants. Recent work suggests that mutations in cis-regulatory mutations should play critical roles in the evolution of this (as well as other) important adaptive traits, but thus far there has been little evidence that directly links regulatory mutations to evolutionary change at the species level. While several genes have previously been shown to affect natural variation in flowering time in Arabidopsis thaliana, most either show protein-coding changes and/or are found at low frequency (o5%). Here we identify and characterize natural variation …

Sequence-Based Analysis Of Structural Organization And Composition Of The Cultivated Sunflower (Helianthus Annuus L.) Genome, Navdeep Gill, Matteo Buti, Nolan Kane, Arnaud Bellec, Nicolas Helmstetter, Hélène Berges, Loren Rieseberg

Biology Faculty Articles

Sunflower is an important oilseed crop, as well as a model system for evolutionary studies, but its 3.6 gigabase genome has proven difficult to assemble, in part because of the high repeat content of its genome. Here we report on the sequencing, assembly, and analyses of 96 randomly chosen BACs from sunflower to provide additional information on the repeat content of the sunflower genome, assess how repetitive elements in the sunflower genome are organized relative to genes, and compare the genomic distribution of these repeats to that found in other food crops and model species. We also examine the expression …

Variation In Arabidopsis Flowering Time Associated With Cis-Regulatory Variation In Constans, Ulises Rosas, Yu Mei, Qiguang Xie, Joshua A. Banta, Royce W. Zhou, Gabriela Seufferheld, Silvia Gerard, Lucy Chou, Naeha Bhambhra, Jennifer Deane Parks, Jonathan M. Flowers, C. Robertson Mcclung, Yoshie Hanzawa, Michael D. Purugganan

Dartmouth Scholarship

The onset of flowering, the change from vegetative to reproductive development, is a major life history transition in flowering plants. Recent work suggests that mutations in cis-regulatory mutations should play critical roles in the evolution of this (as well as other) important adaptive traits, but thus far there has been little evidence that directly links regulatory mutations to evolutionary change at the species level. While several genes have previously been shown to affect natural variation in flowering time in Arabidopsis thaliana, most either show protein-coding changes and/or are found at low frequency (<5%). Here we identify and characterize natural variation in the cis-regulatory sequence in the …

A Multi-Faceted Approach To Improving Adamts13 Deficiency Prediction Algorithm, Steven Nevers, David Brandt, Matthew Biggs

Journal of Undergraduate Research

Despite previous work by our group and other researchers, the existing methods to predict ADAMTS13 deficiency are inadequate. Through the use of machine learning techniques applied to mined patient data we have improved the accuracy and efficiency of our existing ADAMTS13 deficiency prediction algorithm. In conjunction with ARUP Laboratories, who pioneered the original work, we have continued to improve the algorithm accuracy. Significant correlation for severe ADAMTS13 deficiency was seen for four of the observed variables: indirect bilirubin, reticulocyte percentage, creatinine, and platelet count; a fifth variable, D-dimer, just missed significance but performed well.