Biology Commons^™

Jellyfish Identification And Quantification In The San Francisco Estuary, Amalia Borson, Lindsay L. Sullivan, Wim Kimmerer

STAR Program Research Presentations

As potential predators and competitors of plankton-eating fish, jellyfish have the potential to negatively impact fish populations. Jellyfish were collected weekly with plankton tows from the RombergTiburonCenterpier in Tiburon, CA. Since some jellyfish were too small to identify, one tow was collected and preserved to record abundances, and a second tow was collected to rear jellyfish until distinguishing characteristics were visible enough for identification. Jellyfish in the preserved tows were then identified, measured, and counted, and their abundance (number m^-3) was calculated. Jellyfish from the second tows were reared in plastic buckets that were lightly bubbled using aquarium …

Preservation Of Biosignature Molecules In Potential Sample Return Container Of The Mars 2020 Mission, Kimberly E. Lykens, Fei Chen Ph.D

STAR Program Research Presentations

Preservation of Biosignature Molecules in Potential Sample Return Container of the Mars 2020 Mission

Kimberly Lykens¹ and Fei Chen²

¹Wittenberg University, Springfield, Ohio 45501 ²Jet Propulsion Laboratory, Pasadena, California, 91109

One requirement for sustainable life on terrestrial planets includes the presence of organic polymers, compounds that are essential for major biological functions such as replication and catalysis. An identified goal of the Mars mission in the year 2020 is to implement a sample-return to identify and validate signs of life on Mars through the discovery of biosignature molecules in Martian core samples. Martian core samples recovered …

Behavioral Variation Between Two Clades Of Leptasterias Spp., Ashley N. Contreras

STAR Program Research Presentations

Leptasterias spp. are six-rayed sea stars found along the rocky intertidal of the northeast Pacific Alaska to Santa Catalina Island, southern California. In central California, three clades of Leptasterias are found in separate or mixed populations, in diverse habitats that range from shallow pools of seagrass and algae to bare rock exposed to crashing waves. Initial field observations of two clades from different locations suggested that behavioral variation may relate to habitat differences among clades. To measure differences in activity, the righting response was timed at both field sites. As a result of behavioral variation observed in the field, more …

Temporal Variation In Larval Release In Botrylloides Violaceous, Damion J. Delton, Sarah Cohen

STAR Program Research Presentations

Despite Botrylloides violaceous being a globally invasive species in temperate marine habitats, little is known about it’s natural dispersal behavior. Previous work (Delton et al, 2011) suggests behavioral inferences based on a related and much more commonly studied species may not be appropriate, potentially due to a large size difference between the non-feeding larvae of these two species. Here, we consider factors that may affect dispersal potential. To study how light affects larval release, we measured timing of larval release in the field and compared larval size and time of release. Colonies were collected from floating docks in Richmond, CA …

Mechanics, Diversity, And Ecology Of Gecko Adhesion, Travis Jay Hagey

Biology

The question of why animals are shaped the way they are has intrigued scientists for hundreds of years. Studies of ecological morphology (the relationship between an organism’s form, function, and environment) often bridge multiple disciplines including biomechanics, ecology, phylogenetics, and comparative methods. In this dissertation, I gathered data and tested hypotheses that considered the link between morphology and performance and the relationship between performance and ecology. I focused my research on the adhesive abilities of geckos. Geckos are an understudied, diverse group of lizards, well known for their adhesive toe pads. I propose that geckos are an excellent group to …

Leatherback Hatchling Fitness, Violet Campbell, Shane Morales, Kelly Stewart

STAR Program Research Presentations

Leatherback hatchling fitness on land is essential for their success in entering the ocean without being predated. Two ways fitness on land may be measured are by how fast hatchlings crawl as well as how fast their righting responses are when they are flipped onto their carapaces. Hatchlings from certain nests have slower righting response times along with slower crawl times, both of which would increase the odds of predation and hinder the success of hatchlings reaching the ocean.

Hatchling crawling speed was determined by timing 12 randomly selected hatchlings from each nest to crawl a distance of two meters. …

Neurotensin And Cholecystokinin Contract Gallbladder Circular Muscle In Chickens, Teresa F. Degolier, D. R. Brown, Gary E. Duke, M. M. Palmer, J. R. Swenson, Robert E. Carraway

Biological Sciences Faculty Publications

The contractile effects of neurotensin (NT) and cholecystokinin octapeptide (CCK-8) on isolated circular smooth muscle strips of chicken gallbladder were investigated. The NT (0.25-300 nM) produced concentration-dependent contractions on smooth muscle with an EC50 of 8.5 nM (95% confidence limits = 5.3-13.6 nM). In comparison, CCK-8 produced concentration-dependent contractions with an EC50 of 13 nM (95% confidence limits of 9-20 nM). There were no statistical differences in contractile responses when comparing NT and CCK-8 at equimolar concentrations. The NT appears to act directly on smooth muscle tissue in the chicken; the contractile responses were not blocked by 10 μM atropine …

Conceptual Shifts Needed To Understand The Dynamic Interactions Of Genes, Environment, Epigenetics, Social Processes, And Behavioral Choices, Fatimah Linda Collier Jackson, Mihai Niculescu, Robert Jackson

Department of Biology Faculty Publications

Social and behavioral research in public health is often intimately tied to profound, but frequently neglected, biological influences from underlying genetic, environmental, and epigenetic events. The dynamic interplay between the life, social, and behavioral sciences often remains underappreciated and underutilized in addressing complex diseases and disorders and in developing effective remediation strategies. Using a case-study format, we present examples as to how the inclusion of genetic, environmental, and epigenetic data can augment social and behavioral health research by expanding the parameters of such studies, adding specificity to phenotypic assessments, and providing additional internal control in comparative studies. We highlight the …

Vegetation Response After Invasive Tamarix Spp. Removal In The Riparian Zone And Semi-Arid Rangeland Ecosystems, Hisham Nagi El Waer

Electronic Theses and Dissertations

Removal of Tamarix spp. (a.k.a. tamarisk, saltcedar, Athel) invasion is often involved in restoration of Western, riparian habitat; however monitoring of vegetation after removal is often neglected and thus opportunity for adaptive management lost. To address this need, I have conducted three and half years of monitoring vegetation response after invasive Tamarix removal in twenty-five sites on the East and Western Colorado, starting fall 2009. I am also comparing six different methodologies: Point intercept, line transect, nearest neighbor, meter-square quadrats, nested Whittaker plots, and densitometer with the objective of developing monitoring protocols that can be used by …

Evolution Of Xenopus Tropicalis Mc2r: Expression And Activation, Perry Victoria Davis

Electronic Theses and Dissertations

The structure and functional relationship between the melanocortin-2 receptor (MC2R) and adrenocorticotropic hormone (ACTH) is the most complex of the melanocortin gene family. Prior studies had been done on amniote tetrapod MC2Rs (e.g., mammals); this study analyzed the expression and activation of MC2R by an anamniote tetrapod, Xenopus tropicalis (xtMC2R). An immunofluorescence approach, done on the expression of xtMC2R in Chinese Hamster Ovary cells (CHO cells), indicated that the trafficking of xtMC2R to the plasma membrane required co-expression with a tetrapod MRAP1(melanocortin-1 receptor accessory protein). A cAMP-reporter assay was used to show that xtMC2R can activated by human ACTH(1-24, but …

Female Rats Display Enhanced Rewarding Effects Of Ethanol That Are Hormone Dependent, Laura O'Dell

Selected Works Temporary Series

Background: Ethanol (EtOH) abuse is a major health and economic concern, particularly for females who appear to be more sensitive to the rewarding effects of EtOH. This study compared sex differences to the rewarding and aversive effects of EtOH using place-conditioning procedures in rats. Methods: Separate groups of adult (male, female, ovariectomized [OVX] female) and adolescent (male and female) rats received EtOH (0, 0.5, 1.0, 2.0 or 2.5 g/kg; i.p.) and were confined to their initially nonpreferred side of our conditioning apparatus for 30 minutes. On alternate days, they received saline and were confined to the other side. Following 5 …

Defining The Requirements For Early Gene Expression In Bacteriophage Hk639, Amanda L. Seaton

Masters Theses & Specialist Projects

Lambdoid phages suppress transcription termination to fully express their genes. Antitermination of early gene expression in most lambdoid phages is mediated by an interaction between the N protein and a number of host-encoded factors. Bacteriophage HK022 does not rely on a protein for antitermination. To promote full expression of early phage genes, the transcripts of the HK022 put sites interact directly with RNA polymerase to convert it to a termination resistant form. Bacteriophage HK639 also uses RNA-mediated antitermination. However, it only possesses a single put-like element in its left operon. Because most lambdoid phages, including HK022, have antiterminator elements in …

Evidence For An Interaction Between Proinsulin C-Peptide And Gpr146, Gina L.C. Yosten, Grant R. Kolar, Lauren J. Redlinger, Willis K. Samson

Biological Sciences Faculty Research & Creative Works

Microvascular diseases, such as retinopathies, neuropathies, and nephropathies, are a devastating consequence of type 1 and type 2 diabetes. The etiology of diabetes-associated microvascular dysfunction is poorly understood, and, likewise, treatment modalities for these disorders are limited. Interestingly, proinsulin C-peptide has been shown to play a protective role against diabetes-associated complications in experimental animals and in diabetic humans and is thus an attractive therapeutic target. However, an important step in the development of C-peptide-based therapeutics is identification of the C-peptide receptor, which is likely a G protein-coupled receptor (GPCR). Using a unique Deductive Ligand- Receptor Matching Strategy, we sought to …

Leaf Mechanical Strength Predicts Physiological Traits Among Three Life History Types In California Chaparral, Taylor S. Stucky, Nicole M. Rodriguez Purcell, Stephen D. Davis

Featured Research

The Santa Monica Mountains of southern California are located in a Mediterranean-type climate region which experiences reoccurring wildfires due to summer drought and Santa Ana winds. Chaparral vegetation, which consists mostly of evergreen, sclerophyllous shrubs, dominates the landscape. Species of chaparral shrubs have evolved three different life history types in response to fire – those that sprout after fire but do not germinate seeds (obligate sprouters = OS), those that do not sprout after fire but reestablish by seed germination (non-sprouters = NS), and those that both sprout and germinate seeds after fire (facultative sprouters = FS). There are two …

Role Of Camkii In Pancreatic Alpha Cells, Brooke A. Buckland

Electronic Theses and Dissertations

Calcium/calmodulin-dependent protein kinase II (CaMKII) has been identified as an important modulator in controlling electrical activity in neurons and the heart; however, a role for CaMKII in pancreatic alpha cell signaling has not been previously reported. Upon activation by calcium/calmodulin, CaMKII phosphorylates proteins involved in intracellular calcium homeostasis by inducing downstream effects such as an increase in AMPA-receptor single channel conductance, potentiation of L-type voltage dependent calcium channels, and enhanced surface expression of inhibitory GABA_A-receptors. In the pancreas, an increase in intracellular calcium drives secretion of glucagon from alpha cells within the Islets of Langerhans. α-cells contain many …

Development Of Tools To Assess The Effects Of Lunasin On Normal Development And Tumor Progression In Drosophila Melanogaster, Gillian E. Jones

Masters Theses & Specialist Projects

Soy contains many bioactive molecules known to elicit anti-cancer effects. One such peptide, Lunasin, has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. In this study we attempt to understand the developmental effects of Lunasin overexpression in vivo and create reagents that will help us understand Lunasin’s anti tumorigenic effects in an intact organism. cDNA encoding lunasin and EGFP-lunasin were cloned into pUAST and microinjected into Drosophila embryos. Tissue-specific overexpression of EGFP-Lun in the resulting transgenic lines was accomplished by crossing transgenics to various GAL4 driver lines. …

Cold Tolerance Of The Eastern Subterranean Termite, Reticulitermes Flavipes (Isoptera: Rhinotermitidae), In Ontario., Matthew W. Clarke, Graham J. Thompson, Brent J Sinclair

Biology Publications

We characterized the cold tolerance of natural populations of the Eastern subterranean termite (Reticulitermes flavipes (Kollar) [Isoptera, Rhinotermitidae]) in southwestern Ontario, Canada. We measured cold tolerance in workers from six colonies of termites established from Pelee Island in Lake Erie, and Point Pelee National Park. The mean critical thermal minimum, at which termites entered chill coma, ranged from 8.1 to 5.7°C. Mean supercooling points (SCP, the temperature at which individuals freeze) ranged from -4 to -4.6°C, and did not differ significantly between colonies, nor was SCP dependent on body size. Individuals survived brief exposure to low temperatures, as long as …

Female Rats Display Enhanced Rewarding Effects Of Ethanol That Are Hormone Dependent, Laura O'Dell

Departmental Papers (Psych)

Background: Ethanol (EtOH) abuse is a major health and economic concern, particularly for females who appear to be more sensitive to the rewarding effects of EtOH. This study compared sex differences to the rewarding and aversive effects of EtOH using place-conditioning procedures in rats. Methods: Separate groups of adult (male, female, ovariectomized [OVX] female) and adolescent (male and female) rats received EtOH (0, 0.5, 1.0, 2.0 or 2.5 g/kg; i.p.) and were confined to their initially nonpreferred side of our conditioning apparatus for 30 minutes. On alternate days, they received saline and were confined to the other side. Following 5 …

Local Recruitment And Natal Dispersal Distances Of American Kestrels, Karen Steenhof, Julie A. Heath

Biology Faculty Publications and Presentations

Variation in recruitment patterns and dispersal behavior can have important consequences for population viability, genetic structure, and rates of evolutionary change. From 1992 to 2006 we studied a marked population of American Kestrels (Falco sparverius) nesting in boxes in southwestern Idaho to identify factors that affect local recruitment and natal dispersal distances. A low proportion (4%) of locally produced kestrels (n = 2180) returned to nest in study area boxes. Offspring of locally produced individuals were 3.1 times more likely to return than offspring of parents that did not hatch in study area boxes and, independent of …

Presence And Function Of Tetrodotoxin In Terrestrial Vertebrates And Invertebrates, Amber N. Stokes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tetrodotoxin (TTX) is a potent neurotoxin found in a variety of species. This toxin has long been of concern to human health as it is found in puffer fish, which are a delicacy in Japan. Since the distribution of this toxin is so great, there are many questions regarding the evolution and ecology of organisms that have TTX. My research has focused on further investigating three topics with this research: production, predation, and identification of novel TTX bearing taxa. In order to perform this research I first refined a Competitive Inhibition Enzymatic Immunoassay methodology to quantify levels of TTX in …

The Ecology And Genetics Of Schoenoplectus Maritimus, An Important Emergent Macrophyte, Across Diverse Hydrologic Conditions—Implications For Restoration, Amanda Clare Sweetman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wetlands in the Intermountain West are typically dominated by large monotypic stands of emergent wetland plants, are highly productive, and support millions of migratory birds as important stops along the Pacific Flyway. In systems with low species diversity, such as these, diversity within a species (intraspecific diversity) can play an important role in population fitness and ecosystem functioning and can impact restoration success. Our research was designed to inform future restoration and management activities by studying the pattern of diversity within and among natural plant populations, and by studying how hydrology and plant materials used in restoration (source and diversity …

Utilizing Remote Sensing And Geospatial Techniques To Determine Detection Probabilities Of Large Mammals, Patricia A. Terletzky-Gese

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Whether a species is rare and requires protection or is overabundant and needs control, an accurate estimate of population size is essential for the development of conservation plans and management goals. Wildlife science has traditionally relied on human observers in airplanes, helicopter, or ground vehicles to count the number of individuals seen during wildlife surveys. However, these traditional surveys of wildlife require significant resources, are difficult to conduct quickly and safely over remote and/or extensive locations, are disruptive to the studied species, and are prone to significant error due to unobserved or missed animals and multiple counts of single animals. …

Studies On The Cnidocyst Of The Atlantic Sea Nettle, Chrysaora Quinquecirrha, Ivonne Lozano

Theses, Dissertations and Culminating Projects

Jellyfish envenomations have increased drastically in recent years and have been known to cause a wide variety of reactions to the victim. These reactions can be as mild as minor skin irritation to the extreme of death. Many research groups have studied the wide variety of toxins contained within these organisms trying to understand the underlying mechanism that causes these dramatic changes noted to both the physiology and viability of cells. With no clear method for cnidocyst (nematocyst) extraction or lysis, these toxins cannot be recovered entirely, which would hinder research on potential mechanisms of action of the proteins found …

Reproductive Isolation And Cryptic Introgression In A Sky Island Enclave Of Appalachian Birds, Gene D. Sattler, Michael J. Braun

Faculty Publications and Presentations

Reproductive isolation is central to the speciation process, and cases where the strength of reproductive isolation varies geographically can inform our understanding of speciation mechanisms. Although generally treated as separate species, Black-capped chickadees (Poecile atricapillus) and Carolina chickadees (P. carolinensis) hybridize and undergo genetic introgression in many areas where they come into contact across the eastern United States and in the northern Appalachian Mountains. The Great Smoky Mountains harbor the last large breeding population of atricapillus in the southern Appalachians, isolated from the species’ main range by nearly 200 km. This population is believed to be reproductively isolated from local …

Pcr Amplification And Dna Sequence Analysis Of The 45s Ribosomal Dna Cassette Of Chrysaora Quinquecirrha, Caitlin Michelle Burns

Theses, Dissertations and Culminating Projects

The recent overpopulation of jellyfish occurring in the Barnegat Bay of New Jersey has been of growing concern. One prolific species, Chrysaora quinquecirrha, has been the subject of genetic research to determine the relatedness amongst populations and therefore determine its spread. In this study, a partial nucleotide sequence of the 45S ribosomal DNA cassette from C. quinquecirrha genomic DNA of Barnegat Bay was identified and compared to other cnidarian species. Approximately 44.8% of the cassette was identified, 3465 bp total. A partial 18S rDNA sequence was generated of 1772 bp (96% total). Complete ITS1, 5.8S rDNA, and ITS2 sequences were …

The Effect Of Temperature On The Reproductive Success Of Male Waterbugs (Belostoma Flumineum), Michael Philip Dmytriw

Theses, Dissertations and Culminating Projects

Sexual selection theory predicts that the sex that shows a greater parental investment in the offspring should be limiting to the reproductive success of the opposite sex. Males of the giant waterbug Belostoma flumineum exhibit greater parental investment than females by providing all of the parental care for the eggs, which are firmly attached to their dorsal surface. While male backspace availability can be limiting to female reproductive success, it has previously been found to be more limiting during the spring than during the fall, due to newly emerged males being able to breed more quickly than newly emerged females. …

Solubilization Of Sirolimus In Aqueous Solution Through Complexation With Metal Ions, Ralph A. Falen

Theses, Dissertations and Culminating Projects

Sirolimus is one of the most successful immunosuppressant drugs available but it has an extremely low solubility in water that dramatically limits its oral bioavailability to approximately 20%. This study is aimed to increase solubility of Sirolimus in aqueousolutions through complexation with a metal ion. Using computer modeling and UV absorbance spectroscopy, it was demonstrated that 1) Sirolimus interacts with AJ³⁺,Cu²⁺, Fe³⁺ , Mn²⁺, Zn²⁺ ; 2) this interaction decreases UV light absorbance of Sirolimus measured at ^=280 nm; 3) a previously reported value of solubility of Sirolimus in water is underestimated; …

Heterochromatin Protein 1a (Hp1a) Partner Specificity Is Determined By Critical Amino Acids In The Chromo Shadow Domain And C-Terminal Extension, Deanna Lynn Mendez, Rebecca Mandt, Sarah C.R. Elgin

Biology Faculty Research

Drosophila melanogaster Heterochromatin Protein 1a (HP1a) is an essential protein critical for heterochromatin assembly and regulation. Its chromo shadow domain (CSD) homodimerizes, a requirement for binding protein partners that contain a PXVXL motif. How does HP1a select among its many different PXVXL-containing partners? HP1a binds tightly to Heterochromatin Protein 2 (HP2), but weakly to PIWI. We investigated differences in homodimerization and the impact of the C-terminal extension (CTE) by contrasting HP1a to its paralogue, HP1b. HP1a and HP1b differ in the dimerization interface, with HP1a having an Arg at position 188 rather than Glu. We find that while this substitution …

The Effects Of Calcium Concentration And Food Levels On Daphnia, Fawn Goodberry

Biology Theses

Many physiological processes of Daphnia are negatively affected at low calcium concentrations. The concentration of calcium within lake water influences how Daphnia populations will survive and reproduce in natural environments. An experiment was designed to test the effects of calcium concentration and food level on the growth and reproduction of a Daphnia hybrid. Daphnia pulex x Daphnia pulicaria were reared in a soft water medium at three calcium concentrations (2.5mg/L, 1.0mg/L, 0.50mg/L) and high and low food levels (5.6 x 10⁵ and 5.6 x 10⁴ cells of the algae Ankistrodesmus sp.) in a 2 x 3 factorial …

A Framework For Understanding Noise Impacts On Wildlife: An Urgent Conservation Priority, Clinton D. Francis, Jesse R. Barber

Biology Faculty Publications and Presentations

Anthropogenic noise is an important environmental stressor that is rapidly gaining attention among biologists, resource managers, and policy makers. Here we review a substantial literature detailing the impacts of noise on wildlife and provide a conceptual framework to guide future research. We discuss how several likely impacts of noise exposure have yet to be rigorously studied and outline how behavioral responses to noise are linked to the nature of the noise stimulus. Chronic and frequent noise interferes with animals’ abilities to detect important sounds, whereas intermittent and unpredictable noise is often perceived as a threat. Importantly, these effects can lead …