Biology Commons^™

Characterization Of G-Patch Motif Contribution To Prp43 Function In The Pre-Messenger Rna Splicing And Ribosomal Rna Biogenesis Pathways, Daipayan Banerjee

Theses and Dissertations--Biology

The DExD/H-box protein Prp43 is essential for two biological processes: nucleoplasmic pre-mRNA splicing and nucleolar rRNA maturation. The biological basis for the temporal and spatial regulation of Prp43 remains elusive. The Spp382/Ntr1, Sqs1/Pfa1 and Pxr1/Gno1 G-patch proteins bind to and activate the Prp43 DExD/H box-helicase in pre-mRNA splicing (Spp382) and rRNA processing (Sqs1, Pxr1). These Prp43-interacting proteins each contain the G-patch domain, a conserved sequence of ~48 amino acids that includes 6 highly conserved glycine (G) residues. Five annotated G-patch proteins in baker’s yeast (i.e., Spp382, Pxr1, Spp2, Sqs1 and Ylr271) and with the possible exception of the uncharacterized Ylr271 …

The Regulation And Packaging Of Synaptic Vesicles Related To Recruitment Within Crayfish And Fruit Fly Neuromuscular Junctions: Variations In Low- And High-Output Terminals, Wenhui Wu

Theses and Dissertations--Biology

Glutamate is the main excitatory neurotransmitter in the CNS and at the neuromuscular junctions (NMJs) of invertebrate. The characteristic similarities to CNS glutamatergic synapses in vertebrate and the anatomical simplicity of invertebrate NMJs favor the investigation of glutamatergic synaptic functions in this system. This dissertation mainly aimed to physiologically separate two functional vesicle groups, the reserve pool (RP) and readily releasable pool (RRP) within presynaptic nerve terminals of Procambarus Clarkii and Drosophila melanogaster. This was addressed in part by blocking the vesicular glutamate transporter (VGlut) with bafilomycin A1. Various frequencies of motor nerve stimulation, exposure time, and concentration of …

Factors Influencing The Establishment And Survival Of Native Hardwood Tree Seedlings Of The Kentucky Inner Bluegrass Blue Ash-Oak Savanna-Woodland, James D. Shaffer

Theses and Dissertations--Biology

Historically, the Kentucky Inner Bluegrass blue ash-oak savanna-woodland was the primary ecosystem of the Inner Bluegrass Region (IBR) of Kentucky. After European settlement, the majority (>99%) of Bluegrass savanna was converted to agricultural and urban land uses. Currently remnant savanna tree species are failing to recruit. Therefore, a long-term restoration ecology project researching competition and disturbance on seedling establishment, survival, and growth has been established at Griffith Woods (the largest remaining savanna in Kentucky) in Harrison Co., KY. Fourteen native hardwood tree species (a total of 6,168 seedlings) have been experimentally planted. Light, soil, surrounding vegetation, and herbivory, factors …

Physical Dormancy In Seeds, With Special Reference To Geraniaceae: Morpho-Anatomy, Development, Physiology, Biomechanics And Classification Of Water-Gap Complexes, Nalin Suranjith Gama Arachchige

Theses and Dissertations--Biology

The primary aims of this dissertation were to (1) identify and characterize the water-gap complex in seeds of Geraniaceae, (2) investigate its role in physical dormancy (PY) break and (3) develop a new classification system for water-gap complexes in seeds of angiosperms. The winter annuals Geranium carolinianum and G. dissectum were selected as the main representative species for the study, and seeds of an additional 29 species from the Geraniaceae were used to compare the water-gap complex within the family. A new classification system for water-gap complexes in species with PY was developed by comparing the morpho-anatomical features of PY …

The Role Of Patch Size, Isolation, And Forest Condition On Pileated Woodpecker Occupancy In Southwestern Ohio, Anna Lynn Kamnyev

Browse all Theses and Dissertations

No studies of Pileated Woodpeckers (Dryocopus pileatus) have been done in southwestern Ohio where agriculture is prevalent and forests are significantly fragmented. The objective of this study was to determine the forest fragment size, isolation, and structure preferred by D. pileatus for breeding habitat. I sampled 37 forest fragments varying in size and isolation for D. pileatus cavities and forest characteristics and used LiDAR remote sensing data to analyze forest complexity. I hypothesized that D. pileatus relative abundance would increase with forest fragment size, density of dead trees, and forest vertical complexity but decrease with isolation. The hypotheses that size …

Candida Albicans Czf1 And Efg1 Coordinate The Response To Farnesol During Quorum Sensing, White-Opaque Thermal Dimorphism, And Cell Death, Melanie L. Langford, Jessica C. Hargarten, Krista D. Patefield, Elizabeth Marta, Jill R. Blankenship, Saranna Fanning, Kenneth W. Nickerson, Audrey L. Atkin

School of Biological Sciences: Faculty Publications

Quorum sensing by farnesol in Candida albicans inhibits filamentation and may be directly related to its ability to cause both mucosal and systemic diseases. The Ras1-cyclic AMP signaling pathway is a target for farnesol inhibition. However, a clear understanding of the downstream effectors of the morphological farnesol response has yet to be unraveled. To address this issue, we screened a library for mutants that fail to respond to farnesol. Six mutants were identified, and the czf1Δ/czf1Δ mutant was selected for further characterization. Czf1 is a transcription factor that regulates filamentation in embedded agar and also whiteto- opaque switching. We found …

Four Distinct Types Of Dehydration Stress Memory Genes In Arabidopsis Thaliana, Yong Ding, Ning Liu, Laetitia Virlouvet, Jean-Jack Riethoven, Michael E. Fromm, Zoya Avramova

School of Biological Sciences: Faculty Publications

Background: How plants respond to dehydration stress has been extensively researched. However, how plants respond to multiple consecutive stresses is virtually unknown. Pre-exposure to various abiotic stresses (including dehydration) may alter plants’ subsequent responses by improving resistance to future exposures. These observations have led to the concept of ‘stress memory’ implying that during subsequent exposures plants provide responses that are different from those during their first encounter with the stress. Genes that provide altered responses in a subsequent stress define the ‘memory genes’ category; genes responding similarly to each stress form the ‘non-memory’ category.

Results: Using a genome-wide …

Heritability And Inter-Population Differences In Lipid Profiles Of Drosophila Melanogaster, Cornelia J. F. Scheitz, Yu Guo, Angela M. Early, Lawrence G. Harshman, Andrew G. Clark

School of Biological Sciences: Faculty Publications

Characterizing and understanding the complex spectrum of lipids in higher organisms lags far behind our analysis of genome and transcriptome sequences. Here we generate and evaluate comprehensive lipid profiles (>200 lipids) of 92 inbred lines from five different Drosophila melanogaster populations. We find that the majority of lipid species are highly heritable, and even lipids with odd-chain fatty acids, which cannot be generated by the fly itself, also have high heritabilities. Abundance of the endosymbiont Wolbachia, a potential provider of odd-chained lipids, was positively correlated with this group of lipids. Additionally, we show that despite years of laboratory …

Regional Contingencies In The Relationship Between Aboveground Biomass And Litter In The World’S Grasslands, Lydia R. O’Halloran, Elizabeth T. Borer, Eric W. Seabloom, Andrew S. Macdougall, Elsa E. Cleland, Rebecca L. Mcculley, Sarah Hobbie, W. Stanley Harpole, Nicole M. Decrappeo, Chengjin Chu, Jonathan D. Bakker, Kendi F. Davies, Guozhen Du, Jennifer Firn, Nicole Hagenah, Kirsten S. Hofmockel, Johannes Knops, Wei Li, Brett A. Melbourne, John W. Morgan, John L. Orrock, Suzanne M. Prober, Carly J. Stevens

School of Biological Sciences: Faculty Publications

Based on regional-scale studies, aboveground production and litter decomposition are thought to positively covary, because they are driven by shared biotic and climatic factors. Until now we have been unable to test whether production and decomposition are generally coupled across climatically dissimilar regions, because we lacked replicated data collected within a single vegetation type across multiple regions, obfuscating the drivers and generality of the association between production and decomposition. Furthermore, our understanding of the relationships between production and decomposition rests heavily on separate meta-analyses of each response, because no studies have simultaneously measured production and the accumulation or decomposition of …

Conformational B-Cell Epitope Prediction On Antigen Protein Structures: A Review Of Current Algorithms And Comparison With Common Binding Site Prediction Methods, Bo Yao, Dandan Zheng, Shide Liang, Chi Zhang

School of Biological Sciences: Faculty Publications

Accurate prediction of B-cell antigenic epitopes is important for immunologic research and medical applications, but compared with other bioinformatic problems, antigenic epitope prediction is more challenging because of the extreme variability of antigenic epitopes, where the paratope on the antibody binds specifically to a given epitope with high precision. In spite of the continuing efforts in the past decade, the problem remains unsolved and therefore still attracts a lot of attention from bioinformaticists. Recently, several discontinuous epitope prediction servers became available, and it is intriguing to review all existing methods and evaluate their performances on the same benchmark. In addition, …

Widespread Selection Across Coding And Noncoding Dna In The Pea Aphid Genome, Ryan D. Bickel, Joseph P. Dunham, Jennifer A. Brisson

School of Biological Sciences: Faculty Publications

Genome-wide patterns of diversity and selection are critical measures for understanding how evolution has shaped the genome. Yet, these population genomic estimates are available for only a limited number of model organisms. Here we focus on the population genomics of the pea aphid (Acyrthosiphon pisum). The pea aphid is an emerging model system that exhibits a range of intriguing biological traits not present in classic model systems. We performed low-coverage genome resequencing of 21 clonal pea aphid lines collected from alfalfa host plants in North America to characterize genome-wide patterns of diversity and selection. We observed an excess …

Predicting Invasion In Grassland Ecosystems: Is Exotic Dominance The Real Embarrassment Of Richness?, Eric W. Seabloom, Elizabeth T. Borer, Yvonne Buckley, Elsa E. Cleland, Kendi Davies, Jennifer Firn, W. Stanley Harpole, Yann Hautier, Eric Lind, Andrew Macdougall, John L. Orrock, Suzanne M. Prober, Peter Andler, Juan Alberti, T. Michael Anderson, Jonathan D. Bakker, Lori A. Biederman, Dana Blumenthal, Cynthia S. Brown, Lars A. Brudvig, Maria Caldeira, Chengjin Chu, Michael J. Crawley, Pedro Daleo, Ellen I. Damschen, Carla M. D'Antonio, Nicole M. Decrappeo, Chris R. Dickman, Guozhen Du, Philip A. Fay, Paul Frater, Daniel S. Gruner, Nicole Hagenah, Andrew Hector, Aveliina Helm, Helmut Hillebrand, Kirsten S. Hofmockel, Hope C. Humphries, Oscar Iribarne, Virginia L. Jin, Adam Kay, Kevin P. Kirkman, Julia A. Klein, Johannes M. H. Knops, Kimberly J. La Pierre, Laura M. Ladwig, John G. Lambrinos, Andrew D. B. Leakey, Qi Li, Wei Li, Rebecca Mcculley, Brett Melbourne, Charles E. Mitchell, Joslin L. Moore, John Morgan, Brent Mortensen, Lydia R. O'Halloran, Meelis Partel, Jesus Pascual, David A. Pyke, Anita C. Risch, Roberto Salguero-Gomez, Mahesh Sankaran, Martin Schuetz, Anna Simonsen, Melinda Smith, Carly Stevens, Lauren L. Sullivan, Glenda M. Wardle, Elizabeth M. Wolkovich, Peter D. Wragg, Justin Wright, Louie Yang

School of Biological Sciences: Faculty Publications

Invasions have increased the size of regional species pools, but are typically assumed to reduce native diversity. However, global-scale tests of this assumption have been elusive because of the focus on exotic species richness, rather than relative abundance. This is problematic because low invader richness can indicate invasion resistance by the native community or, alternatively, dominance by a single exotic species. Here, we used a globally replicated study to quantify relationships between exotic richness and abundance in grass-dominated ecosystems in 13 countries on six continents, ranging from salt marshes to alpine tundra. We tested effects of human land use, native …

Delayed Amyloid Plaque Deposition And Behavioral Deficits In Outcrossed Aβpp/Ps1 Mice, Brian A. Couch, Meghan E. Kerrisk, Adam C. Kaufman, Haakon B. Nygaard, Stephen M. Strittmatter, Anthony J. Koleske

School of Biological Sciences: Faculty Publications

Alzheimer’s disease (AD) is a progressive neurodegenerative dementia characterized by amyloid plaque accumulation, synapse/dendrite loss, and cognitive impairment. Transgenic mice expressing mutant forms of amyloid-β precursor protein (AβPP) and presenilin-1 (PS1) recapitulate several aspects of this disease and provide a useful model system for studying elements of AD progression. AβPP/PS1 mice have been previously shown to exhibit behavioral deficits and amyloid plaque deposition between 4–9 months of age. We crossed AβPP/PS1 animals with mice of a mixed genetic background (C57BL/6 × 129/SvJ) and investigated the development of AD-like features in the resulting outcrossed mice. The onset of memory-based behavioral impairment …

Deer Mouse Hemoglobin Exhibits A Lowered Oxygen Affinity Owing To Mobility Of The E Helix. Corrigendum, Noriko Inoguchi, Jake R. Oshlo, Chandrasekhar Natarajan, Roy E. Weber, Angela Fago, Jay F. Storz, Hideaki Moriyama

School of Biological Sciences: Faculty Publications

In the article by Inoguchi et al. (2013) the affiliation for two of the authors, Angela Fago and Roy E. Weber, was given incorrectly. The correct affiliation is Zoophysiology, Department of Bioscience, Aarhus University, Aarhus, Denmark.

Plant Mitochondrial Genome Evolution Can Be Explained By Dna Repair Mechanisms, Alan C. Christensen

School of Biological Sciences: Faculty Publications

Plant mitochondrial genomes are notorious for their large and variable size, nonconserved open reading frames of unknown function, and high rates of rearrangement. Paradoxically, the mutation rates are very low. However, mutation rates can only be measured in sequences that can be aligned—a very small part of plant mitochondrial genomes. Comparison of the complete mitochondrial genome sequences of two ecotypes of Arabidopsis thaliana allows the alignment of noncoding as well as coding DNA and estimation of the mutation rates in both. A recent chimeric duplication is also analyzed. A hypothesis is proposed that the mechanisms of plant mitochondrial DNA repair …

Pronounced Genetic Structure In A Highly Mobile Coral Reef Fish, Caesio Cuning, In The Coral Triangle, Amanda S. Ackiss, Shinta Pardede, Eric D. Crandall, Ma Carmen A. Ablan-Lagman, Ambariyanto, November Romena, Paul H. Barber, Kent E. Carpenter

Biological Sciences Faculty Publications

The redbelly yellowtail fusilier Caesio cuning has a tropical Indo-West Pacific range that straddles the Coral Triangle, a region of dynamic geological history and the highest marine biodiversity on the planet. Previous genetic studies in the Coral Triangle indicate the presence of multiple limits to connectivity. However, these studies have focused almost exclusively on benthic, reef-dwelling species. Schooling, reef-associated fusiliers (Perciformes: Caesionidae) account for a sizable portion of the annual reef catch in the Coral Triangle, yet to date, there have been no indepth studies on the population structure of fusiliers or other mid-water, reef-associated planktivores across this region. We …

Identifying Requirements For The Invasion Of A Tick Species And Tick-Borne Pathogen Through Ticksim, Holly Gaff, Robyn Nadolny

Biological Sciences Faculty Publications

Ticks and tick-borne diseases have been on the move throughout the United State over the past twenty years. We use an agent-based model, TICKSIM, to identify the key parameters that determine the success of invasion of the tick and if that is successful, the success of the tick-borne pathogen. We find that if an area has competent hosts, an initial population of ten ticks is predicted to always establish a new population. The establishment of the tick-borne pathogen depends on three parameters: the initial prevalence in the ten founding ticks, the probability that a tick infects the longer-lived hosts and …

Cooperative Breeding And Long-Distance Dispersal: A Test Using Vagrant Records, Caroline L. Rusk, Eric L. Walters, Walter D. Koenig

Biological Sciences Faculty Publications

Cooperative breeding is generally associated with increased philopatry and sedentariness, presumably because short-distance dispersal facilitates the maintenance of kin groups. There are, however, few data on long-distance dispersal in cooperative breeders-the variable likely to be important for genetic diversification and speciation. We tested the hypothesis that cooperative breeders are less likely to engage in long-distance dispersal events by comparing records of vagrants outside their normal geographic range for matched pairs (cooperatively vs. non-cooperatively breeding) of North American species of birds. Results failed to support the hypothesis of reduced long-distance dispersal among cooperative breeders. Thus, our results counter the conclusion that …

In The News, Joe N. Caudell

Human–Wildlife Interactions

No abstract provided.

In Vitro And In Silico Characterization Of Solanum Lycopersicum Wound-Inducible Proteinase Inhibitor-Ii Gene, Faiza Munir, Syed Muhammad Saqlan Naqvi, Tariq Mahmood

Turkish Journal of Biology

Plant proteinase inhibitors (PIs) are antimetabolic defensive proteins conferring resistance in plants against a variety of competing organisms such as bacteria, viruses, fungi, attacking nematodes, and insects. In the fields of plant biochemistry and molecular genetics research, tremendous success has been achieved in generating transgenic crops that have defensive approaches against biotic challenges. In this study, in vitro and in silico analysis was carried out for a wound-inducible PI-II gene isolated from 4 selected varieties (Roma, Nagina, Moneymaker, and Rio Grande) of Solanum lycopersicum L. Around 684 bp of PI-II gene was amplified, sequenced, translated, modeled to protein structure, and …

Identification And Molecular Characterization Of A Receptor-Like Protein Kinase Gene From Corchorus Capsularis, Kh. Ambareen Reza, Sazia Sharmin, Mahdi Muhammad Moosa, Niaz Mahmood, Ajit Ghosh, Rifat Ara Begum, Haseena Khan

Turkish Journal of Biology

A leucine-rich repeat-containing protein kinase (LRR-PK) gene has been identified in Corchorus capsularis. This gene, named CcLRR-RLK1, is 3032 bp long and encodes a protein composed of 958 amino acid residues. The deduced peptide contains 3 distinctive domains: a transmembrane domain, 5 leucine-rich repeat-containing motifs, and a serine/threonine protein kinase domain. Expression analysis indicated that the gene is reduced by several environmental stresses including high salt, fungus, and abscisic acid, suggesting its possible involvement in multiple stress response pathways.

Phylogenetic Analysis Of Rhizoctonia Solani Ag-4 Isolates From Common Beans In Black Sea Coastal Region, Turkey, Based On Its-5.8s Rdna, Meli̇ke Çebi̇ Kiliçoğlu, İbrahi̇m Özkoç

Turkish Journal of Biology

In this study, 114 Rhizoctonia solani isolates were obtained from diseased common bean (Phaseolus vulgaris L.) plants from the Black Sea coastal region of Turkey. Genetic variation was determined among R. solani anastomosis group 4 (AG-4) subgroups (AG-4 HG-I and AG-4 HG-II). ITS-5.8 rDNA sequences of these isolates were aligned with other known R. solani sequences from GenBank, and distance and parsimony analysis were used to determine phylogenetic relationships. The R. solani AG-4 isolates were placed in 2 main lineages, corresponding to AG-4 HG-I and AG-4 HG-II. Based on phylogenetic analysis, genetic variations were shown in the AG-4 subgroups (especially …

Compositional Studies And Antioxidant Potential Of Albizia Lebbeck (L.) Benth. Pods And Seeds, Muhammad Zia-Ul-Haq, Shakeel Ahmad, Mughal Qayum, Sezai̇ Erci̇şli̇

Turkish Journal of Biology

Albizia lebbeck (L.) Benth. is an important medicinal tree found in Pakistan. The present study evaluates the composition and antioxidant potential of various parts of the A. lebbeck plant. Compositional studies indicated carbohydrates as major components while saponin was found as a major antinutrient in both pods and seeds. Potassium was found in the highest amount and copper in the lowest. The amino acid profile indicated that arginine and lysine are present in excessive amounts in seeds while glutamic acid and aspartic acid are present in the highest concentrations in pods. While the linoleic acid was detected as the major …

The Effect Of 3-Methylcholanthrene And Butylated Hydroxytoluene On Glycogen Levels Of Liver, Muscle, Testis, And Tumor Tissues Of Rats, Fi̇kri̇ye Polat, Egemen Dere, Eylem Gül, İzzet Yelkuvan, Öztürk Özdemi̇r, Günsel Bi̇ngöl

Turkish Journal of Biology

This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats …

Purification Of Antioxidant Protein Isolated From Peganum Harmala And Its Protective Effect Against Ccl4 Toxicity In Rats, Hanaa Ahmed, Helal Abu Elzahab, Gamia Alswiai

Turkish Journal of Biology

The present study was conducted to determine the protective effect of the purified protein from seeds of Peganum harmala against carbon tetrachloride (CCl_{4})-induced toxicity in male albino rats. The purification steps included ammonium sulfate fractionation and chromatography on DEAE-cellulose, CM-Sepharose, and Superdex 75 columns. The molecular mass of the purified protein was 132 kDa by gel filtration technique; it consisted of 2 subunits with molecular masses of 30.199 kDa and 38.018 kDa by SDS-PAGE. Results of the dose-dependent experiment with purified protein prior to CCl_4 administration were higher at 4 mg/kg body weight. The antioxidant activity of the purified protein …

Salicylic Acid Delays Leaf Rolling By Inducing Antioxidant Enzymes And Modulating Osmoprotectant Content In Ctenanthe Setosa Under Osmotic Stress, Mehmet Demi̇ralay, Aykut Sağlam, Asi̇m Kadioğlu

Turkish Journal of Biology

The effects of salicylic acid (SA) on leaf rolling under osmotic stress in Ctenanthe setosa (Roscoe) Eichler were evaluated. The leaves were pretreated with SA, paclobutrazol (PAC), and antioxidant enzyme inhibitors for 15 h and then kept in polyethylene glycol (PEG) for 4 h to promote osmotic stress. The degree of leaf rolling was measured every hour during the 4-h PEG treatment. Antioxidant enzymes (superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase) and NADPH oxidase (NOX) activities, endogenous SA contents, hydrogen peroxide, proline, and soluble sugar were determined immediately after PEG treatment. SA treatment retarded leaf rolling and induced antioxidant …

Karyotype Traits In Romanian Selections Of Edible Blue Honeysuckle, Elena Truta, Gabriela Vochita, Craita Maria Rosu, Maria Magdalena Zamfirache, Zenovia Olteanu, Lacramioara Oprica

Turkish Journal of Biology

Large morpho-anatomical and biochemical variability was evidenced in all Lonicera caerulea L. provenances. The knowledge of chromosome constitution is of basic importance in this context of elevated phenotype heterogeneity and represents a necessary step in breeding programs. The Romanian selections are tetraploid (2n = 4x = 36). Chromosomes are small-sized (

Antimicrobial, Antioxidant, And Synergistic Properties Of Two Nutraceutical Plants: Terminalia Catappa L. And Colocasia Esculenta L., Sumitra Chanda, Kalpna Rakholiya, Komal Dholakia, Yogesh Baravalia

Turkish Journal of Biology

Antibiotics have been effective in treating infectious diseases, but resistance to these drugs has led to the emergence of new and reemergence of old infectious diseases. Using a combination of plant extracts and antibiotics is one way of combating these multidrug-resistant microorganisms. The aim of this investigation was to evaluate the antimicrobial and antioxidant properties of 2 nutraceutical plants: Terminalia catappa and Colocasia esculenta. The antimicrobial activity of the plants was evaluated against 25 microorganisms by agar well diffusion method. C. esculenta did not show any antimicrobial activity against bacteria and fungi. Therefore, only methanol extract of T. catappa was …

A Holistic Approach For Selection Of Bacillus Spp. As A Bioremediator For Shrimp Postlarvae Culture, Thimmalapura Devaraja, Sanjoy Banerjee, Fatimah Yusoff, Mohamed Shariff, Helena Khatoon

Turkish Journal of Biology

Indigenous Bacillus pumilus, B. licheniformis, and B. subtilis were isolated from marine water and soil samples and investigated for potential bioremediation ability in Penaeus monodon culture. Bacillus spp. were selected based on their wide range of growth conditions, ease of mass culture, tolerance to total ammonia nitrogen (TAN), inhibition of pathogenic vibrios, nonpathogenicity, and ability to reduce TAN. Results showed that optimum growth of the selected Bacillus spp. occurred at 30 °C, pH 7.5, and 1.5% NaCl, and they secreted protease, amylase, and lipase. Vibrio spp. were also inhibited by 3 Bacillus spp. In addition, the selected Bacillus spp. had …

Using Seedlessness-Related Molecular Markers In Grapevine Breeding For Seedlessness Via Marker-Assisted Selection Into Muscat Of Hamburg × Sultani Progeny, Murat Akkurt, Ati̇lla Çakir, Mi̇na Shi̇dfar, Fi̇li̇z Mutaf, Gökhan Söylemezoğlu

Turkish Journal of Biology

The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) × Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as …