Biology Commons^™

Pcr Amplification And Dna Sequence Analysis Of The Ss-Actin Gene From A Scyphozoan Jellyfish (Chrysaora Quinquecirrha), Lorraine Malkowitz

Theses, Dissertations and Culminating Projects

The scyphozoan jellyfish Chrysaora quinquecirrha (CQ) is becoming a major pest of Eastern bays and waterways due to changing environmental conditions, including the warming of inlet waters, man-made plastic bulkheads and reduced plant life which serve as havens for their predators. Their increasing numbers warrants investigations into population control of these animals. Therefore any information about them may help in understanding and eventually controlling jellyfish blooms.

ß-actin is a ubiquitous cytoskeletal protein that has many essential functions. The protein and nucleotide sequences are highly conserved across all species, yet the number, size and sequences of the introns are highly variable. …

Characterization Of Human Icer Promoter And Comparative Studies Of Its Activity In Human Prostate Cancer Cells, Dipika Bhagvanji Patel

Theses, Dissertations and Culminating Projects

The Inducible cAMP Early Repressor (ICER) is a dominant transcriptional repressor that binds to cAMP responsive elements (CRE) located in the promoter of a target gene to repress cAMP-mediated gene transcription. The promoter of ICER contains four regulatory CRE sequences, termed cAMP autoregulatory response elements (CAREs) that are highly inducible by cAMP signaling. In a cell, cAMP pathway induces the expression of ICER, however, when ICER protein level increases above normal level, it can repress its own production by binding to these CARE sites located in its promoter region. ICER is normally present in neuroendocrine cells, however, it is not …

Expression Of Suppressor Of Cytokine Signaling 2 (Socs2) During Optic Nerve Regeneration In Xenopus Laevis, Rupa Priscilla Choudhary

Legacy Theses & Dissertations (2009 - 2024)

The optic nerve and other central nervous system (CNS) axons tend to lose their capacity to regenerate following an injury in adult amniotes, but these axons are able to regenerate throughout life in anamniotes. The long-term goal of my study is to identify molecular mechanisms responsible for the retention of this regenerative capacity. Specifically, I will examine the role of the gene Suppressor of Cytokine Signaling 2 (SOCS2) in the successful regeneration of retinal ganglion cell (RGC) axons of the South African claw-toed frog, Xenopus laevis, after injury.

The Role Of Pkr In Transducing The Ribotoxic Stress Response Elicited By Ricin, Lauren Rose-Boehnlein

Legacy Theses & Dissertations (2009 - 2024)

Ribotoxins such as ricin and T-2 toxin bind to ribosomes and trigger cleavage of 28S rRNA and inhibition of protein synthesis. Upon detection of ribosomal alterations, the cell initiates a ribotoxic stress response (RSR), resulting in activation of the stress-activated protein kinase and mitogen-activated protein kinase (SAPK/MAPK) signaling cascade, upregulated expression of both pro-and anti-apoptotic stress-inducible genes, increased secretion of inflammatory cytokines, and ultimately culminating in apoptosis. While it is not immediately clear the exact mechanism by which ribosomes induce the RSR, nor the exact cellular components involved upstream of MAPK activation, it has recently been proposed, in the case …

Identification Of Epitopes On Ricin Toxin's Enzymatic Subunit (Rta) Critical For Eliciting Neutralizing Antibodies And Protective Immunity, Joanne Marie O'Hara

Legacy Theses & Dissertations (2009 - 2024)

Ricin toxin's enzymatic subunit (RTA) is a 267 amino acid RNA N-glycosidase that selectively depurinates eukaryotic ribosomal RNA and arrests protein synthesis. The crystal structure of RTA revealed that the protein assumes three distinct folding domains (FD). Residues within FD1 and FD2 form RTA's active site pocket and are proposed to interface with ribosomal proteins, while FD3's primary function is to associate with ricin's B subunit (RTB). In this study I sought to identify the regions of RTA that are important in eliciting toxin-neutralizing antibodies (TNA), as this information is critical for current efforts to develop RTA-based subunit vaccines. I …

The C. Elegans Rna-Binding Protein Gld-1 Recognizes Sequence, Structure And Context Information To Repress Translation Of Its Mrna Targets, Jung Hoon Doh

Legacy Theses & Dissertations (2009 - 2024)

Considering the general suppression of transcription during late oogenesis and early embryo development, post-transcriptional control of maternal mRNAs by RNA binding proteins emerges as an important mechanism in controlling late oogenesis and early embryo development. The C. elegans germline offers an ideal system to understand such processes. GLD-1 (GermLine Development defective) is a maxi-KH motif containing RNA binding protein, which controls many different stages during the C. elegans germline development from decision over germ cell proliferation vs. meiosis entry to production of mature gametes, suggesting that GLD-1 likely controls many mRNA targets. gld-1 mutants have variable germline defects, the major …

Micropatterned Electrospun Nanofibrous Substrates As Scaffolds For Engineered Salivary Glands, David Alexander Soscia

Legacy Theses & Dissertations (2009 - 2024)

The salivary gland is a complex organ exhibiting a branching, 3-dimensional structure made up of acinar (saliva-producing), and ductal (saliva transporting and modifying) epithelial cells. The high surface area of the gland allows it to efficiently provide the mouth with saliva, maintaining oral cleanliness and comfort. Salivary gland hypofunction, a significant clinical problem often caused by the autoimmune disease Sjögren's syndrome or head and neck radiation for cancer patients, affects millions of Americans and is characterized by a loss of function of salivary gland acinar cells. Chronic xerostomia, or dry mouth, arises as a result of salivary gland hypofunction and …

Expression And Purification Of The K2p Family Of Ion Channels, Drew Barber

Legacy Theses & Dissertations (2009 - 2024)

The KCNK (two-pore-domain K+ channel) family of potassium channels are the most widely expressed potassium channels in mammals. They are expressed in numerous organs including the brain, heart, adrenal glands, and kidneys. There are fifteen members of this family which can be further divided into six sub groups. These proteins can act as leak channels, setting the rest membrane potential of electrically excitable cells. Due to their function as modulators of excitability these proteins are under control from numerous different chemical and physical signals which include oxygen tension, pH, lipids, mechanical stretch, and neurotransmitters. These channels are not as well …

Developing A Toolkit To Study The Role Of Inteins In Mycobacterial Biology, Rupsa Dey

Legacy Theses & Dissertations (2009 - 2024)

Tuberculosis, caused by Mycobacterium tuberculosis, is one of the most widespread and highly contagious diseases in the world today. This disease affects people from all walks of life and has a devastating effect on immune compromised individuals. Another concerning fact is the emergence of multiple drug resistant and extreme drug resistant strains of the bacilli that has made treatment very difficult. Thus it has become imperative that every avenue should be explored to fight and eradicate this disease. One such unexplored avenue is the role of inteins in mycobacterial biology. Inteins are intervening sequences found in conserved regions of a …

Experimental And Molecular Studies Of Bryophyte Dispersal On Alpine Summits, Sean Charles Robinson

Legacy Theses & Dissertations (2009 - 2024)

A review of bryophyte dispersal studies over the last sixty years revealed that the role of gametophytic fragments in bryophyte population dynamics has not been adequately addressed. In this dissertation, I investigate the dispersal potential of wind blown bryophyte fragments on Adirondack alpine summits using a combination of field and laboratory methods.

Habitat Associations And Occupancy Modeling Of Marsh Birds In The Hudson Valley Region Of Eastern New York, Lauren Jorgensen

Legacy Theses & Dissertations (2009 - 2024)

Populations of many North American marsh birds have declined over the past few decades, a trend commensurate with declines in wetland habitat area. Substantial declines in populations of breeding marsh bird species in New York State are associated with declines in wetland habitat area due to urbanization. The objective of this study was to examine what habitat and landscape features increase the probability of occupancy for nine target marsh bird species in New York State. This research was conducted in the Hudson River Valley region of Eastern New York, a region which is characterized by varied topography and geology, a …

Detection And Epidemiological Patterns Of Drug Resistance Mutations In Nys Plasmodium Falciparum Clinical Malaria Specimens, Jennifer L. Conaway

Legacy Theses & Dissertations (2009 - 2024)

Plasmodium falciparum is a human-infective parasite that causes the most severe form of malaria. Resistance to a wide spectrum of antimalarials has been documented in this species, and single strains may be resistant to several drugs. A new technology, pyrosequencing, has been shown to be useful for the rapid detection of SNPs involved in drug resistance. It can be used in a clinical setting and readily detects new mutants, which frequently appear in this species due to strong selection for antimalarial resistance. In this study, a pyrosequencing protocol was developed to identify mutations in the Plasmodium falciparum genes Pfcrt, Pfdhps, …

Agriculture In New York State : Farm Size And Its Implications To Farmland Protection And Biodiversity Conservation, Julianna Marie Potter

Legacy Theses & Dissertations (2009 - 2024)

In the United States there are 939 million acres of land held by ranchers and farmers. Conservation of this agricultural landscape is critical to the protection of biodiversity. Management of agricultural land is also associated with the success of biodiversity protection efforts in protected areas, including parks and preserves, as well as outside protected areas. Sustained viability of the New York farming sector will enhance and secure biodiversity on farmland, as viable farms will remain part of the landscape.

The Role Of Ess1 In Survival, Morphogenetic Switching And Transcription In The Fungal Pathogen Candida Albicans, Dhanushki Poornima Samaranayake

Legacy Theses & Dissertations (2009 - 2024)

Candida albicans is a fungal pathogen that causes serious infections among immune-compromised patients and premature infants. C. albicans can become drug resistant, therefore, identifying new antifungal drug targets is an important goal. Here, we study a peptidyl-prolyl cis/trans isomerase called Ess1 as a potential drug target. Ess1 is conserved among pathogenic fungi, and therefore, potential inhibitors of Ess1 should display a broad spectrum of activity. We confirm that Ess1 is essential for growth in Candida albicans, but unlike the previously published find, deleting one copy of the C. albicans ESS1 gene did not affect morphogenetic switching. However, further reducing activity …

The Role Of An Rna Binding Protein Hnrnp K During Axon Development And Regeneration In Xenopus Laevis, Yuanyuan Liu

Legacy Theses & Dissertations (2009 - 2024)

Coordinated synthesis and assembly of the cytoskeletal network contribute significantly to morphological changes during axon outgrowth. Previous studies demonstrated that heterogeneous ribonucleoprotein K (hnRNP K), an RNA binding protein, binds to the 3'-untranslated regions of all neurofilament triplet subunits, the most abundant components of the axonal cytoskeleton. These findings raised the hypothesis that hnRNP K post-transcriptionally mediates the coordinated expression of axonal cytoskeletal components. In my thesis, I test this hypothesis during both axonal development and regeneration.

Characterization Of Postsynaptic Calcium(Ii) Signals And Their Role In Synaptic Regulation At The Drosophila Larval Neuromuscular Junction, Sunil Ashok Desai

Legacy Theses & Dissertations (2009 - 2024)

Postsynaptic [Ca2+]i has been proposed to play an important role in both synaptic plasticity and synaptic homeostasis. Postsynaptic Ca2+ signals appear to regulate synaptic transmission at the Drosophila larval NMJ; however, they have not been characterized at a NMJ. We were interested in examining the postsynaptic Ca2+ signals in this system and determining its influence on synaptic strength. The muscle Ca2+ transients were recorded by injecting the muscle fibers with Ca2+ indicators; discrete postsynaptic Ca2+ transients were observed along the NMJ during evoked and spontaneous transmitter release. The magnitude of the Ca2+ signals was correlated with synaptic efficacy, terminals producing …

Experimental Studies Of The Multitrophic Effects Of Anti-Herbivore Defense In Three Pine Barrens Shrub Species, Elizabeth R. Coffey

Legacy Theses & Dissertations (2009 - 2024)

Plants produce numerous chemical defense compounds in response to herbivory. Induced chemical defenses potentially influence soil dynamics by changing the nutrient ratio of the leaves, which are seasonally shed and contribute to the soil, or by affecting the detritus community through leaching of chemicals in the root zone. Manual clipping was utilized in this study to induce chemical compounds with the goal of examining the secondary effects of induced plant defenses. I removed 25% of the mass of fifteen plants of three different plant species in the Albany Pine Bush annually for two consecutive years. I then compared the soil …

Gld-1 Represses Its Puf Mrna Targets Prior To/At Initiation Of Translation In The C.Elegans Germline, Gautham Sarathy

Legacy Theses & Dissertations (2009 - 2024)

The C.elegans germline offers an ideal system to study posttranscriptional regulation of gene expression as it is a major mechanism through which the control over gene expression is achieved. GLD-1 (defective in GermLine Development) is a maxi-KH motif containing RNA binding protein that controls various aspects of germline development from decision over germcell proliferation vs. meiotic entry to the production of mature gametes suggesting that GLD-1 likely controls many mRNA targets.

Intra And Interhost Dynamics Shaping Arbovirus Adaptation And Evolution, Alexander T. Ciota

Legacy Theses & Dissertations (2009 - 2024)

Arthropod-borne viruses (arboviruses), which are predominately mosquito-borne and almost exclusively RNA viruses, are maintained in nature in complex transmission cycles involving blood sucking invertebrates and vertebrate hosts. Although over 120 arboviruses are human pathogens responsible for causing a significant and expanding global health burden, a detailed understanding of the complex interactions between these pathogens and their hosts, particularly invertebrate hosts, is lacking. Defining these interactions is necessary if we are to understand the selective pressures and, therefore, evolutionary, adaptive, and epidemiological potential of arboviruses. This requires experimental infection and evolution studies, particularly in vivo, with natural hosts. The results presented …

Effects Of Lead Exposure On Synaptic Function, Shoily Parveen Khondker

Legacy Theses & Dissertations (2009 - 2024)

Abstract

Potential Of Marine Bacteria As A Source Of New Biofilm Formation Inhibiting Compounds, Jane Lee Han

UNF Undergraduate Capstone Projects and Honors Theses

New antibacterial drugs are needed to keep up with the alarming increase in infections and incidence. Historically, the majority of clinically useful drugs have been obtained from terrestrial natural sources such as plants and microorganisms. Today such terrestrial sources of drugs are diminishing, owing to rediscovery of known compounds. Fortunately marine microorganisms are in an emerging and underdeveloped source of novel compounds with promising pharmaceutical potential.

An Unbiased Approach To Identify Genes Involved In Development In A Turtle With Temperature-Dependent Sex Determination, Jena L. Chojnowski, Edward L. Braun

Natural Sciences Faculty Publications

Many reptiles exhibit temperature-dependent sex determination (TSD). The initial cue in TSD is incubation temperature, unlike genotypic sex determination (GSD) where it is determined by the presence of specific alleles (or genetic loci). We used patterns of gene expression to identify candidates for genes with a role in TSD and other developmental processes without making a priori assumptions about the identity of these genes (ortholog-based approach). We identified genes with sexually dimorphic mRNA accumulation during the temperature sensitive period of development in the Red-eared slider turtle (Trachemys scripta), a turtle with TSD. Genes with differential mRNA accumulation in response to …

Introducing Environmental Toxicology In Instructional Labs: The Use Of A Modified Amphibian Developmental Toxicity Assay To Support Inquiry-Based Student Projects, Roger Sauterer, James R. Rayburn

Research, Publications & Creative Work

Introducing students to the process of scientific inquiry is a major goal of high school and college labs. Environmental toxins are of great concern and public interest. Modifications of a vertebrate developmental toxicity assay using the frog Xenopus laevis can support student-initiated toxicology experiments that are relevant to humans. Teams of students formulate hypotheses, perform experiments, analyze data, and present their results. By performing experiments to investigate the toxicity of household chemicals, pharmaceuticals, or agricultural chemicals, students will gain an appreciation of the environmental effects of improper disposal of common chemicals and industrial or agricultural run-off.

A Molecular Perspective On The Ageing Of Marine Dissolved Organic Matter, R. Flerus, O. J. Lechtenfeld, B. P. Koch, S. L. Mccallister, P. Schmitt-Kopplin, Ronald Benner, G. Kattner

Faculty Publications

Dissolved organic matter (DOM) was extracted by solid-phase extraction (SPE) from 137 water samples from different climate zones and different depths along an eastern Atlantic Ocean transect. The extracts were analyzed with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) with electrospray ionization (ESI). Δ14C analyses were performed on subsamples of the SPE-DOM. In addition, the amount of dissolved organic carbon was determined for all water and SPE-DOM samples as well as the yield of amino sugars for selected samples. Linear correlations were observed between the magnitudes of 43 % of the FT-ICR mass peaks and the extract Δ14C …

Investigation Of A 16s Rna Central Domain Pseudoknot, Jenna Marie Jasinski-Bolak

Wayne State University Theses

X-ray crystallography of the prokaryotic 30S ribosomal subunit revealed a myriad of complex RNA-RNA, RNA-protein, and protein-protein interactions. Among these are several phylogenetically conserved RNA pseudoknots. Pseudoknots are structurally and functionally diverse RNA secondary structures. They are generally formed by two short complimentary sequences separated by many bases of single stranded regions or loops. These relatively simple folds are often yield complex structures that are key components of functionally important conformational changes in RNA structure. One such pseudoknot is located in the central domain of the 16S rRNA.

The central domain pseudoknot is formed by Watson-Crick base pairing between G570-C866 …

The Prdm Gene Apoptix Antagonizes Programmed Cell Death In Tribolium, Zahabiya Husain

Wayne State University Theses

Previous studies in holometabolous insects have shown that programmed and cell proliferation play important roles in insect metamorphosis. To elucidate the function of the newly identified Tribolium Prdm gene Apoptix (Apox) I performed a detailed analysis of Apox knockdown effects in the embryo, the pupa and the adult eye. My results revealed that Apox is required for the survival of retinal tissue after onset of differentiation and also generally in tissues which experience high amounts of proliferation and differentiation during pupal development. Further, combinatorial knockdown of Apox and initiator caspases produced evidence that Apox specifically protects from programmed cell death …

2b: "Copy Of Carbon Dioxide Data Illinois Natural Gas", U.S. Energy Information Administration

Ecosystem Disruption & Climate Change

No abstract provided.

2b: "Copy Of Carbon Dioxide Data Illinois Totals", U.S. Energy Information Administration

Ecosystem Disruption & Climate Change

No abstract provided.

2b: "Copy Of Carbon Dioxide Data Illinois Petroleum Products", U.S. Energy Information Administration

Ecosystem Disruption & Climate Change

No abstract provided.

Developmental Expression Of Drop-Dead Is Required For Early Adult Survival And Normal Body Mass In Drosophila Melanogaster, Christine Lynn Sansone, Edward M. Blumenthal

Biological Sciences Faculty Research and Publications

In Drosophila melanogaster, mutations in the gene drop-dead (drd) result in early adult lethality, with flies dying within 2 weeks of eclosion. Additional phenotypes include neurodegeneration, tracheal defects, starvation, reduced body mass, and female sterility. The cause of early lethality and the function of the drd protein remain unknown. In the current study, the temporal profiles of drd expression required for adult survival and body mass regulation were investigated. Knockdown of drd expression by UAS-RNAi transgenes and rescue of drd expression on a drd mutant background by a UAS-drd transgene were controlled with the Heat Shock Protein …