Biology Commons^™

Object Neophilia In Domestic Purebred Dogs, Lydia Kniowski

Senior Honors Theses

Neophilia is defined as a preference for novelty. This characteristic has been described in a variety of animal species, and may have been a contributing factor in the domestication of dogs. This study tested three purebred dog breeds for neophilia with inanimate objects. Observations of dogs’ selections when presented with two familiar toys and a novel toy were analyzed. Novel toys were preferred in 60% of selections presenting a significant neophilic trend (P=0.002). Of the breeds analyzed, Labradors selected novel toys 53% of the time, while Brittanys preferred them in 67% of selections. Although both breeds showed a …

Functional Morphology Of The Spiracle And Surrounding Structures Of The Spiny Dogfish Shark, Squalus Acanthias, Clint Mcferren

Honors Capstones

No abstract provided.

Long‐Term Survival Of Escherichia Coli In The Environment And Growth Advantage In Stationary Phase, Elizabeth Mengis

Honors Capstones

No abstract provided.

Phylogeny And Phylogeography Of Mantophryne (Anura: Microhylidae) Reveals Cryptic Diversity In New Guinea, Lauren Oliver

Honors Capstones

No abstract provided.

The Glutamate Effect On The Functionality Of Pol I Dna Polymerases, Mytrang Hoang Do

Honors Capstones

No abstract provided.

Phylogenetic Relationships In Sarraceniaceae Based On Conserved Sequences Generated From Sarracenia Alata, Danielle Fuselier

Honors Capstones

No abstract provided.

Phosphorylation Studies Of Serine 663 On Human 5-Lipoxygenase, Pamela Arden Doerner

Honors Capstones

No abstract provided.

Validation On Sodium Channel Dependence By Electrical Stimulation-Induced Dopamine Release, Alice E. Hernandez ^, Mabel N. Terminel, Edward Castaneda *

COURI Symposium Abstracts, Spring 2012

No abstract provided.

Nutrient Limitation Of Algal Biomass In Streams Of The Sacramento Mountains, New Mexico, Bianca C. Luong^, Vanessa Lougheed*

COURI Symposium Abstracts, Spring 2012

Anthropogenic activities often alter the chemistry and overall quality of freshwater ecosystems. Algae are good indicators of ecosystem health because they respond quickly to influxes of nutrients. In this study, we used algal biomass and nutrient limitation experiments to assess the water quality in streams of the Sacramento Mountains of New Mexico. We sampled 7 sites monthly from May to September 2011, with the exception of June (n = 27) for both periphyton (attached algae) and phytoplankton. Phytoplankton algal biomass was significantly related to phosphorus in the water column; while there were no relationships between periphyton biomass and nutrients. The …

The Causes And Consequences Of Increased Active Layer Depth In Arctic Tundra Ponds Over The Past 40 Years, Gabriela Contreras ^, Vanessa L. Lougheed *

COURI Symposium Abstracts, Spring 2012

Active layer is used to describe the upper most layer of soil that thaws each summer in the Arctic. With a warming Arctic, permafrost is expected to thaw and active layer depth to increase, thus releasing organic material and nutrients into aquatic environments; however, there are few long-term datasets available to test these predictions. The ponds at the International Biological Program (IBP) site in Barrow, Alaska represent one of the only locations in the Arctic with long-term aquatic ecosystem data. The objective of this study was to determine whether the temperature of tundra ponds has increased over the past 40 …

Sequence-Specific Targeting Of Dosage Compensation In Drosophila Favors An Active Chromatin Context, Artyom Alekseyenko, Joshua Ho, Shouyong Peng, Marnie Gelbart, Michael Tolstorukov, Annette Plachetka, Peter Kharchenko, Youngsook Jung, Andrey Gorchakov, Erica Larschan, Tingting Gu, Aki Minoda, Nicole Riddle, Yuri Schwartz, Sarah C.R. Elgin, Gary Karpen, Vincenzo Pirrotta, Mitzi Kuroda, Peter Park

Biology Faculty Research

The Drosophila MSL complex mediates dosage compensation by increasing transcription of the single X chromosome in males approximately two-fold. This is accomplished through recognition of the X chromosome and subsequent acetylation of histone H4K16 on X-linked genes. Initial binding to the X is thought to occur at "entry sites" that contain a consensus sequence motif ("MSL recognition element" or MRE). However, this motif is only ∼2 fold enriched on X, and only a fraction of the motifs on X are initially targeted. Here we ask whether chromatin context could distinguish between utilized and non-utilized copies of the motif, by comparing …

Effects Of Ailanthus Altissima Soil Leachates On Nodulation And Expression Of Two Genes That Regulate Nodulation Of Trifolium Pratense, Jesse Michael Lincoln

Masters Theses

Ailanthus altissima (Simaroubaceae) is an invasive tree from China that has spread over much of North America. A number of characteristics contribute to its success, notably tolerance of nutrient-poor soils, rapid growth rates, prolific production of wind dispersed seeds, escape from predation, and the production of allelopathic compounds. That Ailanthus altissima frequently co-occurs with legumes in nutrient poor soils alludes to a possible relationship between Ailanthus altissima and nitrogen fixing legumes. Legumes increase the biospheres supply of bio-available nitrogen through facilitating the fixation of atmospheric nitrogen within structures on roots called nodules. Thus, increased nodulation of legumes caused by Ailanthus …

Population-Level Compensation Impedes Biological Control Of An Invasive Forb And Indirect Release Of A Native Grass, Yvette K. Ortega, Dean E. Pearson, Lauren P. Waller, Nancy J. Sturdevant, John L. Maron

Biological Sciences Faculty Publications

The intentional introduction of specialist insect herbivores for biological control of exotic weeds provides ideal but understudied systems for evaluating important ecological concepts related to top-down control, plant compensatory responses, indirect effects, and the influence of environmental context on these processes. Centaurea stoebe (spotted knapweed) is a notorious rangeland weed that exhibited regional declines in the early 2000s, attributed to drought by some and to successful biocontrol by others. We initiated an experiment to quantify the effects of the biocontrol agent, Cyphocleonus achates, on Ce. stoebe and its interaction with a dominant native grass competitor, Pseudoroegneria spicata, under …

Electrochemical Characterization Of The Shell Of The Eastern Oyster, Crassostrea Virginica, Yuhchae Yoon, Andrew S. Mount, Karolyn Hansen, Douglas C. Hansen

Biology Faculty Publications

No abstract provided.

Neurodegeneration - A Means To An End, Amit Singh

Biology Faculty Publications

Cell death, a global phenomenon found throughout the animal kingdom, is a mechanism to maintain tissue homeostasis and for adaptation to changes in the environment [1,2]. Millions of cells die in our body daily- they succumb to stress and commit suicide by a mechanism referred to as cell death or apoptosis [2-4]. Under normal conditions cells are continuously replaced by new cells from the stemor progenitor- cells. For example, an optimum balance in shedding of dead cells from the skin and their replenishment by new ones maintain our health and hygiene. In this context, apoptosis is a mechanism to eliminate …

Trehalose Transporter From African Chironomid Larvae Improves Desiccation Tolerance Of Chinese Hamster Ovary Cells., Nilay Chakraborty, Michael Menze, Heidi Elmoazzen, Halong Vu, Martin Yarmush, Steven Hand, Mehmet Toner

Faculty and Staff Scholarship

Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disaccharide trehalose. Trehalose is known to improve desiccation tolerance in mammalian cells when present on both sides of the cell membrane. Because trehalose is membrane impermeant the development of desiccation strategies involving this promising sugar is hindered. We explored the potential of using a high-capacity trehalose transporter (TRET1) from the African chironomid Polypedilum vanderplanki[21] …

Exploring The Role Of Sonic Hedgehog In The Lymph Heart Development Of Xenopus Laevis, Laura Barry

Honors Theses

Xenopus as a Model Organism in Evolutionary Developmental Biology

Xenopus laevis, the African clawed frog, is an important model organism in the field of evolutionary developmental biology research. These tongue-less aquatic frogs are basally branching anuran amphibians that display marked sexual dimorphism and have webbed hind feet that sport several small, sharp claws. This species is a useful model organism despite its long generation time and genomic complexity, for historical and practical reasons. The animal adapts well to housing in a laboratory setting, and its reproduction can be …

Rcf1 And Rcf2, Members Of The Hypoxia-Induced Gene 1 Protein Family, Are Critical Components Of The Mitochondrial Cytochrome Bc1-Cytochrome C Oxidase Supercomplex, Vera Strogolova, Andrew Furness, Micaela Robb-Mcgrath, Joshua Garlich, Rosemary A. Stuart

Biological Sciences Faculty Research and Publications

We report that Rcf1 (formerly Aim31), a member of the conserved hypoxia-induced gene 1 (Hig1) protein family, represents a novel component of the yeast cytochrome bc₁-cytochrome c oxidase (COX) supercomplex. Rcf1 (respiratory supercomplex factor 1) partitions with the COX complex, and evidence that it may act as a bridge to the cytochrome bc₁ complex is presented. Rcf1 interacts with the Cox3 subunit and can do so prior to their assembly into the COX complex. A close proximity of Rcf1 and members of the ADP/ATP carrier (AAC) family was also established. Rcf1 displays overlapping function with another Hig1-related …

Comparison Of Regional Eggshell Porosity Between The Brood Parasitic Brown-Headed Cowbird (Molothrus Ater) And Its Hosts: The Dickcissel (Spiza Americana), And Two Non-Parasitic Relatives, The Red-Winged Blackbird (Agelaius Phoeniceus) And The Common Grackle (Quiscalus Quiscula)., Brittany Childs

Honors Projects

The Brown-headed Cowbird (Molothrus ater) is a generalist brood parasite that lays eggs in the nests of many host species, including the Dickcissel (Spiza americana) and two non-parasitic relatives: the Red-winged Blackbird (Agelaius phoeniceus) and the Common Grackle (Quiscalus quiscula). Cowbird eggs reportedly hatch sooner than equivalently-sized host eggs, presumably via accelerated embryonic development enabled by a greater eggshell porosity and consequently greater gas exchange. However, the distribution of pores among apical, equatorial and basal eggshell regions within cowbirds and host species is undetermined. I tested the hypothesis that equatorial porosity would …

Treatment Of Aortic Heart Valve Conduit With Glutamine And Heat Shock As A Means To Deter The Constituent Cellular Population From Becoming Apoptotic, Alyce Marie Linthurst Jones

Theses and Dissertations in Biomedical Sciences

Cryopreserved allograft heart valves represent the best solution for a patient with a failing heart valve. However, the constituent cells become apoptotic and within months of transplant the heart valve becomes acellular and the recipient's cells do not repopulate the allograft (3, 51). A strategy to prevent this situation would be to minimize or prevent apoptosis from occurring by strategically altering steps during heart valve processing. Recently it has been demonstrated that: 1) Heat shock protein 70 is a negative modulator of the apoptotic cascade; 2) Cells in culture exposed to hypothermic conditions produce heat shock protein 70 upon rewarming; …

Dispersal Behavior Correlates With Personality Of A North American Fish, Josh E. Rasmussen, Mark C. Belk

Faculty Publications

The process of dispersal is determined by the interaction of individual (intrinsic) traits and environmental (extrinsic) factors. Although many studies address and quantify dispersal, few evaluate both intrinsic and extrinsic factors jointly. We test the relative importance of intrinsic traits (exploration tendency and size) and extrinsic factors (population density and habitat quality) on dispersal of a medium-sized western United States minnow, southern leatherside chub Lepidomeda aliciae. A generalized linear model with a binomial response was used to determine the probability of individuals dispersing one year after tagging. Medium-sized individuals that were more prone to explore novel environments were 10.7 …

Human-Wildlife Interaction In Serengeti And Ngorongoro Districts Of Tanzania: A Case Study On Small Mammals, Fj Magige

Tanzania Journal of Science

In the Serengeti and Ngorongoro Districts, small mammals are said to provide protein and income to the local people. However, they are simultaneously reported to conflict with farming activities. These conflicting aspects have not been investigated there. The present article considers humansmall mammal interactions in six villages adjacent to the protected areas. Data were obtained through questionnaire administration to the local people in the districts. Small mammals were hunted in the two districts for food purposes and some villagers declared that they were earning income from selling small mammals products. Seventy percent of the respondents in Serengeti District claimed that …

The Influence Of Aliphatic Side Chain Of Anacardic Acid On Molecular Recognition Properties Of Imprinted Polymers, Jyn Philip, J Buchweishaija, Ll Mkayula, L Ye

Tanzania Journal of Science

The objective of this work was to determine the influence of the aliphatic side chain of anacardic acid on molecular recognition properties of imprinted polymers made from anacardanyl methacrylate (AnMcr). Salicylic methacrylate (SaMcr), a structural analog of AnMcr, was synthesized and used as a functional monomer to prepare imprinted polymers for comparison with AnMcr-based polymers. Using divinylbenzene (DVB) as a cross linker and racemic propranolol as a model template, irregular monolithic particles of poly(SaMcr-co-DVB)m were synthesized in toluene, and spherical beads of poly(SaMcr-co-DVB)b and poly(AnMcr-co-DVB)b weresynthesized in acetonitrile by precipitation polymerization. Although imprinted irregular monolithic particles, poly(SaMcr-co-DVB)m, tested in toluene …

Isolation And Biophysical Study Of Fruit Cuticles, Subhasish Chatterjee, Sayantani Sarkar, Julia Oktawiec, Zhantong Mao, Olivia Niitsoo, Ruth E. Stark

Publications and Research

The cuticle, a hydrophobic protective layer on the aerial parts of terrestrial plants, functions as a versatile defensive barrier to various biotic and abiotic stresses and also regulates water flow from the external environment.1 A biopolyester (cutin) and long-chain fatty acids (waxes) form the principal structural framework of the cuticle; the functional integrity of the cuticular layer depends on the outer 'epicuticular' layer as well as the blend consisting of the cutin biopolymer and 'intracuticular' waxes.2 Herein, we describe a comprehensive protocol to extract waxes exhaustively from commercial tomato (Solanum lycopersicum) fruit cuticles or to remove epicuticular and intracuticular waxes …

Microbial Biosafety Of Pilot-Scale Bioreactor Treating Mtbe And Tba-Contaminated Drinking Water Supply, Radomir Schmidt, David A. Klemme, Kate M. Scow, Krassimira R. Hristova

Biological Sciences Faculty Research and Publications

A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigella spp., Campylobacter jejuni, Aeromonas hydrophila, Legionella pneumophila, Vibrio cholerae, Yersinia enterocolytica and Mycobacterium avium in both influent and treated waters from the bioreactor. Total bacteria decreased during FBBR treatment. E. coli, Salmonella and Shigella spp., C. jejuni, …

A Drosophila Deg/Enac Subunit Functions Specifically In Gustatory Neurons Required For Male Courtship Behavior, E. Starostina, T. Liu, V. Vijayan, Zheng Zheng , '11, Kathleen King Siwicki, C. W. Pikielny

Biology Faculty Works

Detection of specific female pheromones stimulates courtship behavior in Drosophila melanogaster males, but the chemosensory molecules, cells, and mechanisms involved remain poorly understood. Here we show that ppk25, a DEG/ENaC ion channel subunit required for normal male response to females, is expressed at highest levels in a single sexually dimorphic gustatory neuron of most taste hairs on legs and wings, but not in neurons that detect courtship-inhibiting pheromones or food. Synaptic inactivation of ppk25-expressing neurons, or knockdown of ppk25 expression in all gustatory neurons, significantly impairs male response to females, whereas gustatory expression of ppk25 rescues the courtship behavior of …

Plant Species Richness And Species Area Relationships In A Florida Sandhill, Monica Ruth Downer

USF Tampa Graduate Theses and Dissertations

Pine sandhill are integral pyrogenic communities in the southeastern United States. Though once widespread, habitat destruction, fire suppression and fragmentation have reduced the population to nearly 3%. It is important to learn as much as possible about these unique areas in order to implement best management practices to conserve and restore the existing populations of these communities.

Fire is central to the maintenance of pine sandhill communities and two conceptual hypothesis regarding burn frequency have come to light in maintaining the unique species composition and richness of these areas. The first is the Intermediate Disturbance Hypothesis which suggests that intermediate …

An Alu-Based Phylogeny Of Gibbons (Hylobatidae), James Michael Oldenburg

Honors Capstones

No abstract provided.

Assessing The Link Between Coastal Development And The Quality Of Fish Habitat In Mangrove Tidal Tributaries, Justin Micheal Krebs

USF Tampa Graduate Theses and Dissertations

To assess the potential influence of coastal development on the quality of estuarine habitat for nekton, we characterized land use and the intensity of land development surrounding small tidal tributaries of Tampa Bay. Based on this characterization, we classified tributaries as undeveloped, industrial, urban or man-made (i.e., mosquito-control ditches). Over one-third (37%) of tributaries were determined to be heavily developed, while fewer than one-third (28%) remain relatively undeveloped. We then examined the nekton community from eleven tributaries in watersheds representing the defined land-use classes. Whereas mean nekton density and species richness were both independent of land use, nekton-community structure differed …

Integration Of Subthreshold And Suprathreshold Excitatory Barrages Along The Somatodendritic Axis Of Pyramidal Neurons, Hysell V. Oviedo, Alex D. Reyes

Publications and Research

Neurons integrate inputs arriving in different cellular compartments to produce action potentials that are transmitted to other neurons. Because of the voltage- and time-dependent conductances in the dendrites and soma, summation of synaptic inputs is complex. To examine summation of membrane potentials and firing rates, we performed whole-cell recordings from layer 5 cortical pyramidal neurons in acute slices of the rat’s somatosensory cortex. We delivered subthreshold and suprathreshold stimuli at the soma and several sites on the apical dendrite, and injected inputs that mimic synaptic barrages at individual or distributed sites. We found that summation of subthreshold potentials differed from …