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2012

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Articles 1171 - 1200 of 1224

Full-Text Articles in Biology

Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah Jan 2012

Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah

Turkish Journal of Biology

Meningitis due to Neisseria meningitidis and Haemophilus influenzae is a common infectious disease among infants and children that can be fatal in the absence of rapid and proper diagnosis and treatment. Therefore, a rapid and sensitive method for diagnosis is a high priority. The aim of this study was to compare the performance of culture, conventional polymerase chain reaction (PCR), and nested PCR methods for diagnosis of meningitis caused by N. meningitidis and H. influenzae. A total of 106 cerebrospinal fluid samples were collected from infants and children suspected of having meningitis. A portion of these samples were used for …

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Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun Jan 2012

Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun

Turkish Journal of Biology

Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a …

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Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain Jan 2012

Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain

Turkish Journal of Biology

Salinity has adverse effects on plant development and ultimately reduces production. Very few reports on the genes induced by salt stress in Gossypium arboreum are available. In this report salt-stress-responsive genes were screened from cotton leaves using the differential display (DD) technique, and gene expression was compared under control and salt stressed conditions. By using a 107 primer combination, 25 gene fragments were found to be up-regulated in response to salt stress. Out of 25 induced fragments, 12 were rejected as false positives after reamplification and quality control assay. The remaining 13 fragments were selected for cloning and genetic transformation. …

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Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova Jan 2012

Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova

Turkish Journal of Biology

The antioxidant and acetylcholinesterase inhibitory potential of methanol extract from Arnica montana cultivated in Bulgaria was evaluated. For the determination of antioxidant activity 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) free radicals, and ferric reducing antioxidant power (FRAP) assay were used. Modified Ellman's colorimetric method was used for quantitative assessment of acetylcholinesterase inhibition potential. Butylated hydroxytoluene (BHT) and galantamine hydrobromide were used as positive controls, respectively. In addition, total polyphenols and tannins, flavonoids, hydroxycinnamic derivatives, and sesquiterpene lactones were determined using Folin-Ciocalteu reagent, AlCl_3, Na_2MoO_4, and m-dinitrobenzene, respectively. The results demonstrated that Arnica extract has strong antioxidant activity and moderate …

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Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad Jan 2012

Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad

Turkish Journal of Biology

Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry. The present study was undertaken to focus on molecular and phylogenetic analyses of the hemagglutinin-neuraminidase (HN) gene in the Iranian isolates. The nucleotide sequence of the HN (780-903 nt) coding region of 6 Newcastle disease viruses (NDVs), isolated from various outbreaks in Iran, was determined and aligned with previously published sequences of 26 NDV isolates in GenBank; the resulting alignment was then analyzed in terms of genetic variation. The number of nucleotide sequence variations presented by each of the isolates indicated …

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Genetic Diversity And Identification Of Some Turkish Cotton Genotypes (Gossypium Hirsutum L.) By Rapd-Pcr Analysis, Yonca Surgun, Beki̇r Çöl, Betül Bürün Jan 2012

Genetic Diversity And Identification Of Some Turkish Cotton Genotypes (Gossypium Hirsutum L.) By Rapd-Pcr Analysis, Yonca Surgun, Beki̇r Çöl, Betül Bürün

Turkish Journal of Biology

Cotton (Gossypium hirsutum L.) is the leading fiber crop and the second most important oil seed crop in the world. This study aimed to analyze the genetic differences among 9 cotton genotypes of cultivars from Turkey using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. A total of 42 RAPD primers were used to determine polymorphism among the genotypes and 34 of the primers produced scorable results, with 319 RAPD-PCR bands obtained. The rate of polymorphism was calculated to be 18.1%, and genetic similarity between any 2 genotypes ranged from 90.2% to 96.5%. By evaluating the presence and absence …

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Apoplastic And Symplastic Solute Concentrations Contribute To Osmotic Adjustment In Bean Genotypes During Drought Stress, Nesli̇han Saruhan Güler, Aykut Sağlam, Mehmet Demi̇ralay, Asi̇m Kadioğlu Jan 2012

Apoplastic And Symplastic Solute Concentrations Contribute To Osmotic Adjustment In Bean Genotypes During Drought Stress, Nesli̇han Saruhan Güler, Aykut Sağlam, Mehmet Demi̇ralay, Asi̇m Kadioğlu

Turkish Journal of Biology

The present study investigates changes in the inorganic ions, proline, and endogenous abscisic acid (ABA) contents of the apoplastic and symplastic compartments of leaves from drought-tolerant (Yakutiye) and drought-sensitive (Zulbiye) cultivars of the common bean (Phaseolus vulgaris L.). Drought stress caused a decrease in leaf water potential and stomatal conductance in both cultivars. Concentrations of proline in the drought-tolerant and drought-sensitive cultivars increased in response to drought stress in both compartments. The symplastic K^+ concentration decreased in both cultivars. However, the opposite trend was observed concerning K^+ concentrations in the apoplastic areas. While the symplastic Na^+ concentrations significantly decreased in …

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Usefulness Of Ca 15-3 For Breast Or Ovarian Primary Sites In Metastatic Adenocarcinoma Of Pleural Fluid, Serpi̇l Oğuztüzün, Murat Kiliç, Ni̇sa Tandogan, Lati̇f Öztürk Jan 2012

Usefulness Of Ca 15-3 For Breast Or Ovarian Primary Sites In Metastatic Adenocarcinoma Of Pleural Fluid, Serpi̇l Oğuztüzün, Murat Kiliç, Ni̇sa Tandogan, Lati̇f Öztürk

Turkish Journal of Biology

This study was conducted to evaluate the diagnostic value of CA 15-3, mammaglobin, c-erbB-2, estrogen receptor (ER), progesterone receptor (PR), CK 20, and CA 125 for detecting metastatic breast and ovarian carcinoma in pleural fluids. The material under study consisted of 26 pleural effusions from invasive breast and ovarian cancer patients from the Hannover Cytopathology Institute. Expressions of these markers were studied using immunocytochemistry. All of the breast cancer cases studied showed a positive reaction with CA 15-3, while only 36% with mammaglobin, 21% with c-erbB-2, 36% with ER, and 7% with PR were positive. The sensitivities of CK 20, …

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An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh Jan 2012

An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh

Turkish Journal of Biology

A cheese isolate Pediococcus pentosaceus lactic acid bacterium, which has been deposited at the Microbial Type Culture Collection Centre Chandigarh with the accession number MTCC 5151, was tested for anti-Shigella dysenteriae activity and the bacteriocin was characterized. The protein band was observed with tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 23 kDa. This is a new and novel bacteriocin that inhibits S. dysenteriae and has not yet been reported from P. pentosaceus. It was purified on a Sephacryl column and the active fraction specific for anti-Shigella dysenteriae with 23 kDa …

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Karyology Of The Scorzonera L. (Asteraceae) Taxa From Turkey, Esra Marti̇n, Özlem Çeti̇n, Serdar Makbul, Ahmet Duran, Meryem Şeker, Derya Boduroğlu, Bahri̇ye Eşmekaya Jan 2012

Karyology Of The Scorzonera L. (Asteraceae) Taxa From Turkey, Esra Marti̇n, Özlem Çeti̇n, Serdar Makbul, Ahmet Duran, Meryem Şeker, Derya Boduroğlu, Bahri̇ye Eşmekaya

Turkish Journal of Biology

In this study, the karyotypes of 13 taxa of the tribe Lactuceae growing naturally in Turkey were examined. Of the species of the genus Scorzonera L. (Asteraceae), S. laciniata L. subsp. laciniata, S. cana (C.A.Mey.) Hoffm. var. jacquiniana (W.Koch) Chamb., S. suberosa C.Koch subsp. suberosa, S. mollis M.Bieb. subsp. mollis, S. papposa DC., S. lacera Boiss. & Bal., S. elata Boiss., and S. parviflora Jacq. have a diploid chromosome number of 2n = 14. For S. phaeopappa (Boiss.) Boiss., this number is 2n = 28, and for S. eriophora DC., S. pseudolanata Grossh., S. tomentosa L., and S. kotschyi Boiss., …

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Hepatoprotective Effect Of Vitamins C And E Against Gasoline Vapor-Induced Liver Injury In Male Rats, Friday Effiong Uboh, Patrick Ekong Ebong, Henry Dan Akpan, Itoro Friday Usoh Jan 2012

Hepatoprotective Effect Of Vitamins C And E Against Gasoline Vapor-Induced Liver Injury In Male Rats, Friday Effiong Uboh, Patrick Ekong Ebong, Henry Dan Akpan, Itoro Friday Usoh

Turkish Journal of Biology

The protective effect of vitamins C and E against gasoline vapor-induced liver injury was investigated in rats. Liver injury was assessed from the activities of liver function diagnostic indices including serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (\gamma-GT), alkaline phosphatase (ALP) activities, total serum protein (TSP), albumin concentrations, and the histological architectures of the liver tissues of the experimental animals. The results showed that gasoline vapors caused a significant (P = 0.05) higher than that of vitamin C. The results of our study suggest a protective effect of vitamin C and vitamin E against gasoline vapor-induced liver …

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Accumulation Of Poly (3-Hydroxybutyrate) By Microbacterium Barkeri Dsm 20145, Srividya Shivakumar Jan 2012

Accumulation Of Poly (3-Hydroxybutyrate) By Microbacterium Barkeri Dsm 20145, Srividya Shivakumar

Turkish Journal of Biology

A gram-positive bacterium that accumulated poly(3-hydroxybutyric) acid (PHB) was isolated from petrol bunk soil. From its morphological and physiological properties and the nucleotide sequence (about 1.5 kb) of its 16S rDNA, it was identified as Microbacterium barkeri DSM 20145. Based on the available literature, this is the first report of PHB accumulation by Microbacterium sp. The isolate accumulated 0.6 g/L, or 29.7%, of PHB under growth conditions in a nitrogen-deficient medium containing glucose and yeast extract (4:1 ratio), with maximum production by 48 h. The capacity of the isolate to produce PHB under different carbon and nitrogen sources was also …

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Fatty Acid Composition Of The Seeds Of Some Species Of Peucedanum (Kral Otu) From Turkey, Nükhet Akpinar, Sali̇h Görgün, Hüseyi̇n Aşkin Akpulat, Mehmet Ali̇ Akpinar, Apdurahman Aktümsek, Ali̇ Emre Akpinar Jan 2012

Fatty Acid Composition Of The Seeds Of Some Species Of Peucedanum (Kral Otu) From Turkey, Nükhet Akpinar, Sali̇h Görgün, Hüseyi̇n Aşkin Akpulat, Mehmet Ali̇ Akpinar, Apdurahman Aktümsek, Ali̇ Emre Akpinar

Turkish Journal of Biology

The fatty acid compositions of the seeds of Peucedanum ruthenicum, Peucedanum chryseum, Peucedanum palimbioides, Peucedanum obtusifolium, and Peucedanum zedelmeierianum were investigated by gas chromatography (GC). The main fatty acids in the species were found to be palmitic acid (C 16:0, 5.78%-14.68%), oleic acid (C 18:1 \omega-9, 31.28%-68.06%), and linoleic acid (C 18:2 \omega-6, 15.99%-33.74%). Trace amounts of cis-vaccenic acid (C 18:1 \omega-7, 0.82%-1.40%) were found in the seed oils of all of the species investigated.

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Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan Jan 2012

Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan

Turkish Journal of Biology

The essential oils obtained from Anethum graveolens, Foeniculum vulgare collected at fully-mature and flowering stages, Mentha piperita, Mentha spicata, Lavandula officinalis, Ocimum basilicum (green- and purple-leaf varieties), Origanum onites, O. vulgare, O. munitiflorum, O. majorana, Rosmarinus officinalis, Salvia officinalis, and Satureja cuneifolia, as well as the widely encountered components in essential oils (\gamma-terpinene, 4-allylanisole, (-)-carvone, dihydrocarvone, D-limonene, (-)-phencone, cuminyl alcohol, cuminyl aldehyde, cuminol, trans-anethole, camphene, isoborneol, (-)-borneol, L-bornyl acetate, 2-decanol, 2-heptanol, methylheptane, farnesol, nerol, isopulegol, citral, citronellal, citronellol, geraniol, geranyl ester, linalool, linalyl oxide, linalyl ester, \alpha-pinene, \beta-pinene, piperitone, (-)-menthol, isomenthone, carvacrol, thymol, vanillin, and eugenol), were screened for their …

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Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol Jan 2012

Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol

Turkish Journal of Biology

Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus …

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Genetic Variability Of Beauveria Bassiana And Metarhizium Anisopliae Var. Anisopliae Isolates Obtained From The Eastern Black Sea Region Of Turkey, Ali̇ Sevi̇m, Monica Höfte, Zi̇hni̇ Demi̇rbağ Jan 2012

Genetic Variability Of Beauveria Bassiana And Metarhizium Anisopliae Var. Anisopliae Isolates Obtained From The Eastern Black Sea Region Of Turkey, Ali̇ Sevi̇m, Monica Höfte, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae var. anisopliae (Metschnikoff) Sorokin are the most common entomopathogenic fungi used in microbial control programs all over the world. Assessments of the genetic variability of these 2 important species are useful for the development of effective biocontrol strategies and for evaluating the impact of artificial epizootics. In this study, the genetic diversity of 13 B. bassiana and 33 M. anisopliae var. anisopliae strains isolated from the hazelnut-growing region of Turkey was determined by using the amplified fragment length polymorphism (AFLP) and \alpha- and \beta-isoenzyme analyses. Cluster analysis of AFLP data clearly separated both …

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Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta Jan 2012

Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta

Turkish Journal of Biology

The present experiment was undertaken to determine the mutagenic potential of lead on the cytomorphology of Trigonella foenum-graecum L. and to determine the maximum concentration of lead nitrate that induces maximum genetic variability. The seeds were treated with 6 different concentrations (10, 25, 50, 100, 200, and 400 ppm) of lead nitrate. The higher concentrations of lead nitrate significantly reduced plant height, number of branches per plant, pollen fertility, and yield; higher concentrations also caused variation in the plants at the seedling and mature stages. In addition, concentrations affected the pairing of homologous chromosomes and spindle formation. This resulted in …

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Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti Jan 2012

Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti

Turkish Journal of Biology

Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 µg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were grown on direct mushroom bed extract, 0.2% glucose-containing mushroom bed extract, and common production medium for determination of growth and lignolytic activities. Since lignolytic activities were high with the direct mushroom bed extract, this medium was further diluted to 1:1 and 1:2 ratios and …

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A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan Jan 2012

A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan

Turkish Journal of Biology

Mucuna pruriens belongs to the family Papillonaceae. The seeds and leaves of these plants contain a large amount of L-DOPA. L-DOPA (L-3,4-dihydroxyphenylalanine) is one of the most widely used drugs in the treatment of Parkinson disease. Development of an efficient gene transfer method is an absolute requirement for genetically improving Mucuna pruriens and creating plants with more desirable traits. A simple protocol was developed for the Agrobacterium-mediated stable genetic transformation of M. pruriens. Agrobacterium tumefaciens strain EHA 101, containing the vector pCAMBIA1305 and the hptII and GUS plus genes, was used for the gene transfer experiments. Putative transgenic shoots were …

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Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz Jan 2012

Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz

Turkish Journal of Biology

Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high (98.61%) and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that …

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Poly-3-Hydroxybutyrate-Producing Extreme Halophilic Archaeon: Haloferax Sp. Ma10 Isolated From Çamaltı Saltern, İzmir, Ebru Teki̇n, Mustafa Ateş, Özge Kahraman Jan 2012

Poly-3-Hydroxybutyrate-Producing Extreme Halophilic Archaeon: Haloferax Sp. Ma10 Isolated From Çamaltı Saltern, İzmir, Ebru Teki̇n, Mustafa Ateş, Özge Kahraman

Turkish Journal of Biology

İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell …

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Biofilm Formation Comparison Of The Sanipacking® Cooling Tower Fill Material Against Standard Polypropylene Fill Material In A Recirculating Model Water System, İrfan Türetgen, Nazmi̇ye Özlem Şanli Yürüdü, Imke Norden Jan 2012

Biofilm Formation Comparison Of The Sanipacking® Cooling Tower Fill Material Against Standard Polypropylene Fill Material In A Recirculating Model Water System, İrfan Türetgen, Nazmi̇ye Özlem Şanli Yürüdü, Imke Norden

Turkish Journal of Biology

Cooling towers are heat rejection systems that are used in some industrial applications, and they have the potential to develop infectious concentrations of Legionella pneumophila. SANIPACKING® cooling tower fill material and standard polypropylene fill material were compared in terms of biofilm formation potential and anti-Legionella activity within a 4-month period using a laboratory-scale recirculating water system. The recirculating water system was experimentally infected with a L. pneumophila standard strain (ATCC 33152) suspension and operated continuously until all experiments had been completed. Results showed that the L. pneumophila rapidly multiplied in a short time during the study within the model system …

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Genetic Diversity Among Salvia Miltiorrhiza Bunge And Related Species Inferred From Nrdna Its Sequences, Li Zhang, Hong-Xia Zhao, Xing Fan, Meng Wang, Chunbang Ding, Rui-Wu Yang, Zhong-Qiong Yin, Xian-Li Xie, Yong-Hong Zhou, De-Guang Wan Jan 2012

Genetic Diversity Among Salvia Miltiorrhiza Bunge And Related Species Inferred From Nrdna Its Sequences, Li Zhang, Hong-Xia Zhao, Xing Fan, Meng Wang, Chunbang Ding, Rui-Wu Yang, Zhong-Qiong Yin, Xian-Li Xie, Yong-Hong Zhou, De-Guang Wan

Turkish Journal of Biology

To investigate the genetic diversity and phylogenetic relationships of Salvia miltiorrhiza and related species, we analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) region for 7 accessions of Salvia miltiorrhiza and another 23 samples from other taxa within the genus Salvia by maximum parsimony and Bayesian inference analyses. There were 257 variation sites amounting to 40.8% of the total base pairs. All of the data revealed abundant genetic diversity in the genus Salvia. The results showed that the tested materials could be grouped into 3 clusters, and the 3 different occasions in Salvia were clustered into 1 clade, which …

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The Effect Of Salt Stress On Antioxidative Enzymes And Proline Content Of Two Turkish Tobacco Varieties, Özge Çeli̇k, Çi̇men Atak Jan 2012

The Effect Of Salt Stress On Antioxidative Enzymes And Proline Content Of Two Turkish Tobacco Varieties, Özge Çeli̇k, Çi̇men Atak

Turkish Journal of Biology

The aim of this study was to compare the salinity tolerances of 2 oriental tobacco varieties (İzmir Özbaş and Akhisar 97). Salinity stress experiments were performed under both in vitro and in vivo conditions. Seedlings of each variety were subjected to 0, 50, 100, 150, 200, 250, 300, and 350 mM NaCl. Photosynthetic pigment levels, lipid peroxidation rate, total protein content, antioxidant enzyme activities, and proline concentrations were determined for seedlings treated with salt for 14 days. The Akhisar 97 variety was found to be more sensitive to salinity stress than the İzmir Özbaş variety. Although proline is thought to …

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Influence Of Phytophthora Capsici L. Inoculation On Disease Severity, Necrosis Length, Peroxidase And Catalase Activity, And Phenolic Content Of Resistant And Susceptible Pepper (Capsicum Annuum L.) Plants, Esra Koç, Ayşen Sülün Üstün Jan 2012

Influence Of Phytophthora Capsici L. Inoculation On Disease Severity, Necrosis Length, Peroxidase And Catalase Activity, And Phenolic Content Of Resistant And Susceptible Pepper (Capsicum Annuum L.) Plants, Esra Koç, Ayşen Sülün Üstün

Turkish Journal of Biology

This study explored the level of infection caused by different inoculum concentrations (10^2, 10^3, and 10^4 zoospores mL^-^1) of Phytophthora capsici in 3 pepper cultivars at days 2, 4, and 6. The effect that the infection induced on the peroxidase (POX), catalase (CAT), and phenolics of resistant and sensitive seedlings, as well as the defense mechanism against the pathogen, were also investigated. The resistance of PM-702 against the isolate used was high, whereas KM-Hot and DEM-8 displayed sensitive reactions. As a result of analyses of the leaves and stems from the 3 pepper cultivars, important changes in biochemical parameters were …

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Deletion Of Polyphosphate Kinase Gene (Ppk)Has A Stimulatory Effect On Actinorhodin Production By Streptomyces Coelicolor A3(2), İrem Yalim Camci, Tuğrul Doruk, Ümmehan Avi̇can, Sedef Tunca Gedi̇k Jan 2012

Deletion Of Polyphosphate Kinase Gene (Ppk)Has A Stimulatory Effect On Actinorhodin Production By Streptomyces Coelicolor A3(2), İrem Yalim Camci, Tuğrul Doruk, Ümmehan Avi̇can, Sedef Tunca Gedi̇k

Turkish Journal of Biology

Polyphosphate, which is synthesized by the enzyme polyP kinase (PPK), is an important energy and phosphate storage polymer that plays a crucial role in the regulation of adaptive responses of cells to physical and chemical stresses. In this work, the polyphosphate kinase gene (ppk) of Streptomyces coelicolor was deleted, and the effect of this mutation on actinorhodin and undecylprodigiosin biosynthesis was investigated. Deletion of the ppk gene had a stimulatory effect on actinorhodin production; the mutant strain produced about 5 times more antibiotic compared to the wild type strain at 120 h of growth. There was no difference in undecylprodigiosin …

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Calcium Oxalate Crystal Types In Three Oak Species (Quercus L.) In Turkey, Bedri̇ Serdar, Hati̇ce Demi̇ray Jan 2012

Calcium Oxalate Crystal Types In Three Oak Species (Quercus L.) In Turkey, Bedri̇ Serdar, Hati̇ce Demi̇ray

Turkish Journal of Biology

Crystal types from 3 oak species representing 3 different sections of the genus Quercus were identified. Crystals were studied with scanning electron microscopy after localisation by light microscopy. The crystals were composed of calcium oxalate silicates as whewellite (calcium oxalate monohydrate) composites. In Quercus macranthera Fisch. et Mey. subsp. syspirensis (C.Koch) Menitsky (İspir oak) from the white oaks (section Quercus: Leucobalanus), whewellite and trihydrated weddellites coexisted. Axial parenchyma cells included 30 crystal chains with walls as chambers in uniseriate ray cells; some cells had many crystals without any chambers and were small. Long thin crystals were found adherent to the …

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Optimization Of The Process Parameters For The Utilization Of Orange Peel To Produce Polygalacturonase By Solid-State Fermentation From An Aspergillus Sojae Mutant Strain, Hande Demi̇r, Ni̇han Göğüş, Canan Tari, Doreen Heerd, Marcelo Fernandez Lahore Jan 2012

Optimization Of The Process Parameters For The Utilization Of Orange Peel To Produce Polygalacturonase By Solid-State Fermentation From An Aspergillus Sojae Mutant Strain, Hande Demi̇r, Ni̇han Göğüş, Canan Tari, Doreen Heerd, Marcelo Fernandez Lahore

Turkish Journal of Biology

The effect of orange peel concentration, HCl concentration, incubation time and temperature, and inoculum size on the spore count and activity of polygalacturonase (PG) enzyme produced from Aspergillus sojae M3 by solid-state fermentation was screened using 2^k factorial design. Orange peel and HCl concentrations and incubation time were significant factors affecting the responses. Optimum conditions favoring both PG and spore production from Aspergillus sojae M3 were determined as 2% orange peel and 50 mM HCl concentrations at 22 °C and 4.3 days of incubation. An overlay plot was constructed for use as a practical chart for production of high enzyme …

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Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu Jan 2012

Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu

Turkish Journal of Biology

Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants …

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Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin Jan 2012

Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin

Turkish Journal of Biology

The focus of this study was Cyclamen alpinum (formerly C. trochopteranthum). Habitat fragmentation, environmental degradation, and overharvesting of tubers have exerted pressure on native populations of this valuable ornamental species. Although the entire Cyclamen genus is in the Convention on International Trade in Endangered Species of Wild Fauna and Flora-Appendix II (CITES II), no species has yet been red-listed. Estimating the level and distribution of genetic variation in populations of rare and endemic species is important for conserving genetic diversity within a species in the context of well-developed conservation strategies. Currently, DNA markers are the most effective means used to …

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