Open Access. Powered by Scholars. Published by Universities.®

Biology Commons™

Open Access. Powered by Scholars. Published by Universities.®

2012

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 1081 - 1110 of 1224

Full-Text Articles in Biology

Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk Jan 2012

Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk

Turkish Journal of Biology

Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia (At-ext) were investigated using both Allium cepa root meristematic cells and a short-term mutation assay in Salmonella typhimurium with TA98 and TA100 strains in the presence or absence of S9 mix, respectively. In the Allium tests, EC_{50} value was determined as 50 g/L, and 0.5 × EC_{50}, EC_{50}, and 2 × EC_{50} concentrations of At-ext were applied to onion tuber roots. Methyl methane sulfonate (MMS, 10 ppm) and distilled water were used as positive and negative controls, respectively. The mitotic index (MI) increased in all concentrations in …

Go to article

Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi Jan 2012

Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi

Turkish Journal of Biology

Phoenix dactylifera L. was introduced in Tunisia a very long time ago and plays an important socioeconomic role, especially in the south of the country. Genetic diversity and relationships among 26 cultivars were assessed with 7 ISSR primers. A total of 43 amplified bands were obtained. Principal component analyses based on Nei genetic distances showed no geographical separation with the exception of the Dhahbi cultivar, which has a limited geographical distribution. A group of cultivars that are also phonetically clustered was distinguished. These cultivars have a common maturity period and a common fruit consistency. The Mantel test emphasizes a significant …

Go to article

Antioxidant Enzymes And Gst Activity In Natural Populations Of Holandriana Holandrii From The Bosna River, Jelena Vrankovic, Svetlana Labus-Blagojevic, Bela Csanyi, Jarmila Makovinska, Olga Cvetkovic, Zoran Gacic, Dusko Blagojevic, Momir Paunovic Jan 2012

Antioxidant Enzymes And Gst Activity In Natural Populations Of Holandriana Holandrii From The Bosna River, Jelena Vrankovic, Svetlana Labus-Blagojevic, Bela Csanyi, Jarmila Makovinska, Olga Cvetkovic, Zoran Gacic, Dusko Blagojevic, Momir Paunovic

Turkish Journal of Biology

Specimens of the freshwater snail Holandriana holandrii affected by different levels of contamination were collected from 3 sites within the Bosna River Basin, i.e. Visoko, Doboj, and Modrica. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) were investigated in order to understand their variation with respect to the pollution status of the sampling locations. The results revealed a significant increase in CAT, GPx, and GST activities in the snails collected from Modrica, suggesting that the animals at this location are exposed to a higher level of oxidative …

Go to article

Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva Jan 2012

Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva

Turkish Journal of Biology

One of the natural reservoirs of potentially human-pathogenic bacteria is believed to be the rhizosphere. The aim of the present work was to test nontuberculous mycobacterium Mycobacterium phlei MbP18 for its ability to colonize the rhizosphere of wheat and to evaluate its effect on plant growth under saline conditions. In competitive wheat root tip colonization assays, M. phlei MbP18 showed poor competitive colonization of the wheat rhizosphere compared to the reference strain. The strain produced lipase, amylase, cellulase, and pectinase and grew well in the presence of high salt (up to 4% NaCl) and at high temperatures (up to 40 …

Go to article

Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy Jan 2012

Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy

Turkish Journal of Biology

Oxidative stress responses were evaluated in the digestive gland tissue of Perna viridis exposed to water-accommodated fractions (WAFs) of petrol and diesel at 0.5% and 5% concentrations over 5-, 10-, and 15-day periods. Increased lipid peroxidation, protein carbonyl, DNA integrity, and hydrogen peroxide in WAF-exposed specimens are indicative of oxidative damage due to dissolved petroleum extracts. A consistent increase in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione, and ascorbic acid in WAF-exposed specimens signify the importance of antioxidants in protecting the cell against the oxidative damage arising from petroleum contamination. The responses of field specimens collected from …

Go to article

Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu Jan 2012

Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu

Turkish Journal of Biology

Oral squamous cell carcinomas (OSCCs) are the most common type of oral cancers, and high morbidity and mortality are largely attributable to late-stage diagnosis. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl trisulfide (DATS), an oil-soluble organosulfur compound of garlic, was recently reported for its anticancer effect on several types of cancer cells in vitro; however, these anticancer effects are still unknown in human OSCC cells. In the present study, the effects of DATS on OSCC cell invasion in vitro were investigated. Human OSCC cell lines (HSC-2 and Ca9-22 cells) were treated with DATS …

Go to article

Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu Jan 2012

Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu

Turkish Journal of Biology

Glutathione S-transferase (GST) is the one of most widely used affinity tags in biotechnology applications. The present study named a GST gene as the TtGSTz1 gene, characterized with the conserved glutathione binding motif (SSTSWRVRIAL) under a GST zeta subfamily from Tetrahymena thermophila. Phylogenetic analysis of the TtGstz1p protein sequence, with its orthologs from different GST classes, showed that it is a member of the unicellular GSTz monophyletic clade, which is positioned close to the bacterial GSTz clade. Seven codons of the TtGSTz1 gene were first engineered by introducing silent mutations (TAA > CAA or TAG > CAG) to the code for glutamine …

Go to article

A Comparative Study On The Fatty Acid Composition Of The Oils From Five Bupleurum Species Collected From Turkey, Hati̇ce Taner Saraçoğlu, Gökhan Zengi̇n, Mehtap Akin, Apdurahman Aktümsek Jan 2012

A Comparative Study On The Fatty Acid Composition Of The Oils From Five Bupleurum Species Collected From Turkey, Hati̇ce Taner Saraçoğlu, Gökhan Zengi̇n, Mehtap Akin, Apdurahman Aktümsek

Turkish Journal of Biology

The oil content and fatty acid compositions of 5 Bupleurum species (B. intermedium, B. lancifolium, B. croceum, B. rotundifolium, and B. cappadocicum) were determined. Their oil contents ranged from 2.87% to 4.20%. Gas chromatography (GC) was used for the fatty acid composition. Twenty-one fatty acids were identified and quantified by GC. Oleic acid (C18:1) accounted for more than 50% of the total fatty acids in 3 of the Bupleurum species (B. intermedium, B. lancifolium, and B. rotundifolium). The total unsaturated fatty acid contents varied from 67.28% in B. cappadocicum to 87.06% in B. rotundifolium. The total saturated fatty acids represent …

Go to article

Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya Jan 2012

Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya

Turkish Journal of Biology

In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C. The molecular weight of the phytase from Lactobacillus brevis was estimated as 73 kDa and 34 kDa on SDS-PAGE. The optimum activity of Lactobacillus brevis extracellular and intracellular enzymes emerged at 120 °C, pH 3.4 and at 110 °C, pH 3.4, respectively. The extracellular enzyme from Lactobacillus brevis was 91% stable for 5 …

Go to article

Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin Jan 2012

Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin

Turkish Journal of Biology

Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that …

Go to article

Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul Jan 2012

Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul

Turkish Journal of Biology

The genus Fritillaria embraces about 165 taxa in the family Liliaceae. In this study, the molecular phylogenetic relationships between 10 Fritillaria taxa were presented. Fritillaria spp. were collected from their natural habitats, and specimens with different morphological features were obtained via selective breeding. Specimens with the desired characters were presented as promising candidate cultivars for ornamental use. Phylogenetic analysis was based on DNA sequences of the chloroplast trnL-trnF region. The phylogeny was constructed using the neighbor joining, maximum parsimony, maximum likelihood, and Bayesian inference methods. The data showed that the examined Fritillaria spp. were evidently diverged into 2 Fritillaria subgenera. …

Go to article

Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao Jan 2012

Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao

Turkish Journal of Biology

The persistence of endosulfan and intermediate metabolite endosulfan sulfate in the environment and their toxic effects on biota necessitate their removal. This study investigated the bioaugmentation of endosulfan-contaminated soil by fungal inoculant Aspergillus niger ARIFCC 1053. The influence of bioaugmentation by A. niger on endosulfan-contaminated soil was evaluated with the help of change in pH and released chloride, and by thin layer chromatography and gas chromatography analysis. Its effects on soil functionality were monitored by estimating dehydrogenase and arylsulfatase enzyme activities. The endosulfan degradation reached an undetectable level on day 15. The pH of the medium was nearly neutral (6.9) …

Go to article

Genotoxicity Of Bovine Bone-Derived Microhydroxyapatite (Boneplus-Xs), Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇, Mehmet Büyükleyla, Hasan Basri̇ İla, Tuba Canitezer, Mehmet Kürkçü Jan 2012

Genotoxicity Of Bovine Bone-Derived Microhydroxyapatite (Boneplus-Xs), Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇, Mehmet Büyükleyla, Hasan Basri̇ İla, Tuba Canitezer, Mehmet Kürkçü

Turkish Journal of Biology

The aim of this study was to evaluate the genotoxic effect of a bovine bone-derived hydroxyapatite (HA), Boneplus-xs (B-P-XS). The genotoxicity of B-P-XS was investigated by examining the potential of the xenograft (B-P-XS) to induce gene mutation (evaluated using the Ames test), in vitro chromosome aberration (CA), and sister chromatid exchange (SCE) with tests in cultured human peripheral lymphocytes. Counts of reverse mutations in Salmonella typhimurium TA98 and TA100 strains were not found to be significantly increased after treatment with B-P-XS (P > 0.05). The presence of B-P-XS in peripheral blood culture for 24 and 48 h did not lead to …

Go to article

Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin Jan 2012

Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin

Turkish Journal of Biology

Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.

Go to article

Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin Jan 2012

Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin

Turkish Journal of Biology

Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and …

Go to article

Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi Jan 2012

Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi

Turkish Journal of Biology

Hyperthyroidism increases the vulnerability of the heart to ischemia-reperfusion (I/R) injury. Regarding the fact that diazepam also affects cardiac I/R injury, the current study was conducted to investigate the effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart. The animals were divided into 6 groups: the control, hyperthyroid, control diazepam (1 mg/kg), hyperthyroid diazepam (1 mg/kg), control diazepam (5 mg/kg), and hyperthyroid diazepam (5 mg/kg) groups. Langendorff-perfused rat hearts underwent 40 min of global ischemia and 45 min of reperfusion. Different cardiac parameters, including the left ventricular developed pressure, heart rate, coronary flow, and rate pressure product (RPP), were measured. …

Go to article

Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam Jan 2012

Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam

Turkish Journal of Biology

Every living organism on earth owes its viability to its different sizes of nanostructures and the interaction of these structures at the nano size. Nanotechnology gives us an opportunity to understand nanoscale processes in living organisms and interfere with and manipulate them. Today, biocompatible nanosized structures are designed by applying developments in nanotechnology to biomedicine; thus, therapeutic agents are available to reach diseased tissues and even cells. However, it is essential that nanomaterials that would be used for therapeutic aims be targetable to diseased areas and have low toxicity. In addition, these nanomaterials must have high biocirculation and pharmacokinetic properties. …

Go to article

Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab Jan 2012

Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab

Turkish Journal of Biology

The present study was undertaken to examine the effects of exogenous application of ascorbic acid (AA) through different modes on growth and associated biochemical parameters in Saccharum spp. hybrid cv. HSF-240, under salt stress. In a pot experiment, AA was applied through irrigation or foliar-spray at the concentrations of 0.1, 0.5, and 1 mM with or without 100 mM NaCl concentration. Vegetative growth measurements, antioxidant enzyme activities (POD and SOD), and protein and proline contents of plants were recorded to study the effects of these treatments. The presence of salt reduced the growth of sugarcane plants. The AA application not …

Go to article

Biological Screening Of Various Medicinal Plant Extracts For Antibacterial And Antitumor Activities, Fatma Pehli̇van Karakaş, Arzu Yildirim, Arzu Türker Jan 2012

Biological Screening Of Various Medicinal Plant Extracts For Antibacterial And Antitumor Activities, Fatma Pehli̇van Karakaş, Arzu Yildirim, Arzu Türker

Turkish Journal of Biology

Bioassays of 2 types (antibacterial and antitumor) were performed to show the biological activities of 16 different plants grown in Bolu, Turkey: Clinopodium vulgare L. subsp. vulgare L., Salvia verticillata L. subsp. amasiaca (Frey & Bornm.) Bornm., Salvia tomentosa Mill., Mentha pulegium L., Melilotus officinalis (L.) Desr., Melilotus alba Desr., Medicago lupulina L., Galega officinalis L., Xeranthemum annuum L., Cichorium intybus L., Plantago lanceolata L., Plantago major L. subsp. major, Fumaria officinalis L., Galium palustre L., Echium vulgare L., and Sambucus nigra L. For each plant, 3 different extracts (aqueous, ethanol, and methanol) were obtained, and a total of 48 …

Go to article

Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz Jan 2012

Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz

Turkish Journal of Biology

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in …

Go to article

Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur Jan 2012

Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur

Turkish Journal of Biology

In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class …

Go to article

Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural Jan 2012

Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural

Turkish Journal of Biology

Twenty-seven aqueous extracts obtained from 21 plants used in the treatment of respiratory tract infections as folk remedies in Turkey were investigated for their relative total phenolic contents and antioxidant, antibacterial, antimycobacterial, and antifungal activities. Antibacterial and antifungal activities against dermatophytes and yeast-like fungi were determined using the microdilution method. Antibacterial activity of all extracts was more pronounced against gram-positive bacteria than against gram-negative bacteria. These extracts also exhibited higher antifungal activity against Candida krusei than ketaconazole. Six extracts displayed antidermatophytic activity against Epidermophyton floccosum, while only 3 extracts were highly active against Trichophyton rubrum. The extracts showed strong activity …

Go to article

The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal Jan 2012

The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal

Turkish Journal of Biology

This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) alpha, pi, mu, and theta in prostatic acinar adenocarcinoma (PCA), prostatic intraepithelial neoplasia (PIN), and benign prostatic tissues from 19 patients. Relationships between GST isoenzyme expression in benign, PIN, and PCA tissue were examined by the Wilcoxon signed-rank test and clinicopathological data were examined by the Spearman correlation rank test. When the benign, PIN, and PCA tissues from these cases were compared according to their staining intensity, GST alpha, pi, mu, and theta expressions in tumor cells were significantly lower than in benign epithelial cells (P < 0.05). The GST alpha class displayed the lowest level of expression in PIN and PCA. Expression of GST pi was lower in PCA tissue than in PIN and benign epithelial tissue (P < 0.05). We hypothesize that carcinogenesis in the prostate results from impaired cellular handling of mutagenic agents owing to reduction or loss of expression of multiple GST isoenzymes and other detoxifying and antimutagenesis agents. This study confirms the down-regulation of GST isoenzymes in PCA of the prostate and shows that the loss of GST isoenzyme expression is a phenotype associated with malignant transformation. There was no statistical relationship between GST isoenzyme expression and the clinicopathological data (age, Gleason score, and total serum prostate-specific antigen levels) (P > 0.05).

Go to article

Adam10: A Novel Regulator Of Mast Cell Function And Activation, Travis Faber Jan 2012

Adam10: A Novel Regulator Of Mast Cell Function And Activation, Travis Faber

Theses and Dissertations

In this study we show, to our knowledge, the first description of the role ADAM10 plays on mast cells. ADAM10 is abundantly expressed on mast cells both in vitro and in vivo. Its expression is inhibited by IL-10, a suppressive cytokine. siRNA depletion of ADAM10 on bone marrow-derived mast cells (BMMC) caused decreased IL-6 production following IgE cross-linking and also impaired BMMC stem cell factor (SCF)-induced migration through collagen IV. Mast cells and T helper cells (Th cells) in the peritoneum were reduced in ADAM10 KO mice. In addition, ADAM10 KO BMMC produced significantly less of all cytokines measured following …

Go to article

Analysis Of A Wound-Induced Gene Family In Glycine Max, Gena Robertson Jan 2012

Analysis Of A Wound-Induced Gene Family In Glycine Max, Gena Robertson

Masters Theses

Gene families in plants are important in understanding genome evolution indicating when and where genome duplications and segmental duplications occurred as well as subsequent divergence and subfunctionalization. A gene family in Glycine max that encodes a WI12 protein, wound-induced protein, was found to consist of ten genes on five chromosomes. Wound-induced proteins are activated in response to wounding in plants, and the WI12 protein in particular is thought to be involved in cell wall modifications at the wound site. A variety of bioinformatics tools have been used to analyze the expansion of this family in soybean as well as identify …

Go to article

Biochemical Studies Of Abce1, Lynn Sims Jan 2012

Biochemical Studies Of Abce1, Lynn Sims

Electronic Theses and Dissertations

The growth and survival of all cells require functional ribosomes that are capable of protein synthesis. The disruption of the steps required for the function of ribosomes represents a potential future target for pharmacological anti-cancer therapy. ABCE1 is an essential Fe-S protein involved in ribosomal function and is vital for protein synthesis and cell survival. Thus, ABCE1 is potentially a great therapeutic target for cancer treatment. Previously, cell biological, genetic, and structural studies uncovered the general importance of ABCE1, although the exact function of the Fe-S clusters was previously unclear, only a simple structural role was suggested. Additionally, due to …

Go to article

Uncovering Morphological Variation In Light Of Genetic Data Within The Ozark Minnow, Notropis Nubilus, Sara Katherine Holmes Jan 2012

Uncovering Morphological Variation In Light Of Genetic Data Within The Ozark Minnow, Notropis Nubilus, Sara Katherine Holmes

Honors Program Theses

It has been hypothesized that the modern distribution of the Ozark minnow was shaped by the fragmentation of a widespread ancestral population by periodic glaciations during the late Pliocene and early Pleistocene eras (Berendzen et al., 2010). Today, the species comprises three distinct genetic clades that are: upper Mississippi and northern Ozarks, western Ozarks, and southern Ozarks. In light of this hypothesis, the objective of this study is to determine if the morphology of the fish displays differences that parallel the genetic variation. One way to determine these morphological differences within a population is to look at the geometric morphometrics …

Go to article

Feasibility Study Of Trans-Cranial Direct Current Stimulation In Presence Of Brain Tumor, Abhilash Nair Jan 2012

Feasibility Study Of Trans-Cranial Direct Current Stimulation In Presence Of Brain Tumor, Abhilash Nair

Dissertations and Theses

Trans-cranial Direct Current Stimulation has been shown to modulate cortical neuronal activity. Weak constant current is applied to the scalp using electrodes, leading to sub-threshold changes in neuronal membrane potential. Before actual clinical stimulation is performed, such systems are validated by simulating the stimulation and the resulting current flow patterns using finite element solvers. However, the reliability of such models depends upon the accuracy with which the underlying anatomy has been modeled [1]. In earlier studies it has been shown that the current flow patterns within the brain are altered due to the presence of lesioned brain tissues[2]. Here we …

Go to article

Slave Drivers; Increased Foraging By Host Workers Under The Slave-Making Ant Plotomognathus Americanus, Kevin Purce Jan 2012

Slave Drivers; Increased Foraging By Host Workers Under The Slave-Making Ant Plotomognathus Americanus, Kevin Purce

Dissertations and Theses

No abstract provided.

Go to article

The Effects Of Pleistocene Climatic Change On The Phylogeography Of Euploea Mulciber (Lepidoptera; Nymphalidae; Danainae), Elva Yang Jan 2012

The Effects Of Pleistocene Climatic Change On The Phylogeography Of Euploea Mulciber (Lepidoptera; Nymphalidae; Danainae), Elva Yang

Dissertations and Theses

No abstract provided.

Go to article

First Prev Next Last