Biology Commons^™

Spatial And Temporal Expression Profiling Of Cell-Wall Invertase Genes During Early Development In Hybrid Poplar, Thomas Canam, Sarah W.Y. Mak, Shawn D. Mansfield

Faculty Research & Creative Activity

Cell-wall invertase genes are spatially and temporally regulated in several plant species, including Daucus carota L., Lycopersicon esculentum L. and Solanum tuberosum L. However, few studies of cell-wall invertase genes of trees have been conducted, despite the importance of trees as a source of lignocellulosic biopolymers.We identified three putative cell-wall invertase genes in hybrid poplar (Populus alba L. × grandidentata Michx.) that showed higher homology to each other than to cell-wall invertases of other dicotyledonous species, with two of the genes (Pa×gINV2 and Pa×gINV3) appearing as a genomic tandem repeat. These genes are more similar to each other than to …

Phylogenetic Utility Of Ycf1 In Orchids: A Plastid Gene More Variable Than Matk, Kurt M. Neubig, W. Mark Whitten, Barbara S. Carlsward, Mario A. Blanco, Lorena Endara, Norris H. Williams, Michael Moore

Faculty Research & Creative Activity

Plastid DNA sequences have been widely used by systematists for reconstructing plant phylogenies. The utility of any DNA region for phylogenetic analysis is determined by ease of amplification and sequencing, confidence of assessment in phylogenetic character alignment, and by variability across broad taxon sampling. Often, a compromise must be made between using relatively highly conserved coding regions or highly variable introns and intergenic spacers. Analyses of a combination of these types of DNA regions yield phylogenetic structure at various levels of a tree (i.e., along the spine and at the tips of the branches). Here, we demonstrate the phylogenetic utility …

Simulating The Effects Of Wetland Loss And Inter-Annual Variability On The Fitness Of Migratory Bird Species, Jill L. Deppe, James A. Smith

Faculty Research & Creative Activity

Long-distance migratory shorebirds require wetland stopover sites where they can forage and deposit sufficient fat to complete their migration and, in the spring, reproduce. Conservation biologists are concerned that continental-scale reductions in wetland availability and quality due to human disturbance, climate change, and natural drought events are negatively impacting these species by eliminating critical stopovers along migratory flyways. We describe an individual-based migration model driven by remotely sensed land surface data, climate data assimilation models, and biological field data to examine the impact of changing environmental conditions on migration routes, temporal patterns, and fitness. We used an evolutionary programming approach …

Vegetative Anatomy Of Calypsoeae (Orchidaceae), William Louis Stern, Barbara S. Carlsward

Faculty Research & Creative Activity

Calypsoeae represent a small tribe of anatomically little-known orchids with a wide distribution in the Western Hemisphere. Leaves are present in all genera, except Corallorhiza and Wullschlaegelia both of which are subterranean taxa. Stomata are abaxial (ad- and abaxial in Aplectrum) and tetracytic (anomocytic in Calypso). Fiber bundles are absent in leaves of all taxa examined except Govenia tingens. Stegmata are present in leaves of only Cremastra and Govenia. Roots are velamentous, except in filiform roots of Wullschlaegelia. Vegetative anatomy supports a relationship between Wullschlaegelia and Corallorhiza but does not support the grouping of winter-leaved Aplectrum and Tipularia nor proposed …

Phylogenetic Utility Of Ycf1 In Orchids: A Plastid Gene More Variable Than Matk, Kurt Neubig, W. Whitten, Barbara Carlsward, Mario Blanco, Lorena Endara, Norris Williams, Michael Moore

Faculty Research & Creative Activity

Plastid DNA sequences have been widely used by systematists for reconstructing plant phylogenies. The utility of any DNA region for phylogenetic analysis is determined by ease of amplification and sequencing, confidence of assessment in phylogenetic character alignment, and by variability across broad taxon sampling. Often, a compromise must be made between using relatively highly conserved coding regions or highly variable introns and intergenic spacers. Analyses of a combination of these types of DNA regions yield phylogenetic structure at various levels of a tree (i.e., along the spine and at the tips of the branches). Here, we demonstrate the phylogenetic utility …

Accelerated Exchange Of Exon Segments In Viperid Three-Finger Toxin Genes (Sistrurus Catenatus Edwardsii; Desert Massasauga), Robin Doley, Susanta Pahari, Stephen P. Mackessy, Manjunatha Kini

Biological Sciences Faculty Publications

BACKGROUND: Snake venoms consist primarily of proteins and peptides showing a myriad of potent biological activities which have been shaped by both adaptive and neutral selective forces. Venom proteins are encoded by multigene families that have evolved through a process of gene duplication followed by accelerated evolution in the protein coding region. RESULTS: Here we report five gene structures of three-finger toxins from a viperid snake, Sistrurus catenatus edwardsii. These toxin genes are structured similarly to elapid and hydrophiid three-finger toxin genes, with two introns and three exons. Both introns and exons show distinct patterns of segmentation, and the insertion/deletion …

Lack Of Assortative Mating For Tail, Body Size, Or Condition In The Elaborate Monomorphic Turquoise-Browed Motmot (Eumomota Superciliosa), Troy G. Murphy

Biology Faculty Research

Elaborate male and female plumage can be maintained by mutual sexual selection and function as a mate-choice or status signal in both sexes. Both male and female Turquoise-browed Motmot (Eumomota superciliosa) have long tails that terminate in widened blue-and-black rackets that appear to hang, unattached, below the body of the bird. I tested whether mutual sexual selection maintains the Turquoise-browed Motmot’s elaborate tail plumage by testing the prediction that mating occurs in an assortative manner for tail plumage. I also tested whether assortative mating occurs for body size, a potential measure of dominance, and for phenotypic condition, a …

Plant Regeneration From Callus Culture Of Clematis Gouriana Roxb. – A Rare Medicinal Plant, Hanumanaika Raja Naika, Venkatarangaiah Krishna

Turkish Journal of Biology

An in vitro regeneration protocol through stem callus culture has been standardized for the medicinal climber Clematis gouriana. The explant induced callus on MS-medium supplemented with 0.5 to 1.5 mg l^{-1} BAP and 0.1 to 0.5 mg l^{-1} NAA. The optimized callus induction occurred at the concentration of 1.0 mg l^{-1} BAP and 0.3 mg l^{-1} NAA. After initiation of callus, it was immediately transferred to MS medium containing 4.0 mg l^{-1} FAP and 0.5 mg l^{-1} indole-3-butyric acid (IBA). Upon longer incubation for about 2-6 weeks on the same culture medium initiation of shoot buds from the callus mass …

Purinergic Receptors In The Circumvallate Papillae Of Rats, Alana Marselle Montoya

Electronic Theses and Dissertations

P2X2 receptors and P2Y4 receptors are ATP-activated cell surface receptors that gate movement of K+, Na+, and Ca2+. We used immunocytochemistry for P2X2 and P2Y4 receptors as well as taste cell type markers to learn if P2X2 immunoreactivity (IR) is present in nerve processes in close apposition to Type II and/or Type III taste cells. We also tested to see if P2Y4 IR is present in Type II and/or Type III taste cells. Our results indicate that P2X2 is present only in intragemmal nerve processes. P2X2-LIR nerve processes form close contacts with Type II and Type III taste cells. P2Y4 …

Methods For Detection Of Matrix Metalloproteinases As Biomarkers In Cardiovascular Disease, Viorica Lopez-Avila, Juliet Spencer

Biology Faculty Publications

Matrix metalloproteinases (MMPs) are a family of zinc-dependent proteolytic enzymes that degrade extracellular matrix (ECM) components like collagen, fibronectin, and laminin. While this activity is important for normal development, morphogenesis, and wound healing, deregulation of MMP activity has been implicated in a number of cardiovascular diseases, including congenital heart defects, atherosclerosis, myocardial infarction, and congestive heart failure. MMPs are good potential diagnostic indicators of cardiovascular disease, but current detection methods are time consuming and quite laborious. This review will discuss MMP biology, current methods for detection of MMPs from patient samples, and potential new developments in multiplexed analysis of MMPs.

Metacommunity Patterns In Larval Odonates, Shannon J. Mccauley, Christopher J. Davis, Rick A. Relyea, Kerry L. Yurewicz, David K. Skelly, Earl E. Werner

Biological Sciences

The growth of metacommunity ecology as a subdiscipline has increased interest in how processes at different spatial scales structure communities. However, there is still a significant knowledge gap with respect to relating the action of niche-and dispersal-assembly mechanisms to observed species distributions across gradients. Surveys of the larval dragonfly community (Odonata: Anisoptera) in 57 lakes and ponds in southeast Michigan were used to evaluate hypotheses about the processes regulating community structure in this system. We considered the roles of both niche- and dispersal-assembly processes in determining patterns of species richness and composition across a habitat gradient involving changes in the …

Predator Induction Of Spine Length In Larval Leucorrhinia Intacta (Odonata), Shannon Mccauley, Christopher J. Davis, Earl E. Werner

Biological Sciences

Questions: Do larvae of a dragonfly with a broad habitat distribution have longer abdominal spines when they co-exist with fish, and are these differences the result of phenotypic plasticity?
Hypothesis: Phenotypic plasticity will result in larvae having longer spines when they are exposed to cues from predatory fish.
Organism: Larvae of Leucorrhinia intacta (Odonata: Libellulidae).
Research site: Natural ponds and cattle tanks on the E.S. George Reserve in southeast Michigan.
Methods: We compared the morphology of larvae collected from two natural ponds before and after a drought resulted in the extirpation of fish from one pond. We also compared …

Creationism, Catastrophism, And Velikovsky, William D. Stansfield

Biological Sciences

No abstract provided.

Fibronectin In Aging Extracellular Matrix Fibrils Is Progressively Unfolded By Cells And Elicits An Enhanced Rigidity Response, Kristopher Kubow

Selected Works

No abstract provided.

Cave Biology: It’S Not A Job, It’S An Adventure, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Competitive Abilities Of Native Grasses And Non-Native (Bothriochloa Spp.) Grasses, Cheryl D. Schmidt, Rob Channell Ph.D., Karen R. Hickman, Keith Harmoney, William J. Stark Ph.D.

Biological Sciences Faculty Publications

Old World Bluestems (OWB), introduced from Europe and Asia in the 1920s, recently have begun to raise concerns in the Great Plains. Despite suggestion in the late 1950s that OWB were weedy and negatively impacted biological diversity, they were widely introduced throughout the Great Plains for agricultural purposes. Anecdotal evidence suggests that OWB exhibit invasive characteristics that promote competitive exclusion of native species. The objective of our study was to quantify the competitive abilities of two OWB species (Caucasian bluestem; Bothriochloa bladhii (Retz.) S. T. Blake (= Bothriochloa caucasica (Trin.) C. E. Hubb.) and yellow bluestem; Bothriochloa ischaemum (L.) Keng) …

Pison Menkei, A New Crabronid Wasp In Dominican Amber, Daniel J. Bennett, Michael S. Engel

Faculty Publications

A new crabronid wasp species, Pison menkei sp. n., is described and figured from a single female preserved in Early Miocene (Burdigalian) Dominican amber. The fossil is herein distinguished from extant and extinct congeners and regarded as an isolated species removed from modern Neotropical species groups. It is the seventh named fossil Pison and the second from Dominican amber.

Rapid Detection Of Avian Eimeria Species Using Denaturing Gradient Gel Electrophoresis, Alexandra Martynova-Van Kley, A. Syvyk, I. Teplova, M. E. Hume, A. Nalian

Faculty Publications

A denaturing gradient gel electrophoresis (DGGE) assay was developed to rapidly discriminate species of avian Eimeria. Amplification by PCR of the small subunit ribosomal RNA gene (approximately 1,600 nucleotides) with Eimeria genus-specific primers followed by cloning and sequencing allowed us to carry out phylogenetic analyses and identify clone sequences to species level in most cases. Clones were subsequently used to amplify a smaller fragment (approximately 120 nucleotides) suitable for DGGE. The fragments were separated on denaturing gradient gel and bands with unique migration distances were mixed to obtain an identification ladder. The identification ladder and PCR products obtained from …

Denaturing Gradient Gel Electrophoresis As A Tool To Determine Batch Similarity Of Probiotic Cultures Of Porcine Cecal Bacteria, Michael Hume, Charles M. Scanlan, Roger B. Harvey, Kathleen Andrews, James D. Snodgrass, Armen Nalian, Alexandra Martynova-Van Kley, David Nisbet

Faculty Publications

A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

Sexual Selection And Alternative Reproductive Tactics In Cyprinodon Elegans, Jennifer M. Gumm

Faculty Publications

In systems with alternative reproductive tactics (ARTs), males have discrete behaviors and morphologies that allow them to optimize their reproductive success in relation to others in the population. Males with different phenotypes directly compete with one another for mates, providing unique opportunities for studying social interactions within a species. Using the Comanche Springs pupfish, Cyprinodonelegans, a species with three alternative reproductive tactics, I examine how male behaviors and social interactions influence the reproductive success of each tactic.

Previous studies suggest that large territorial males have high reproductive success, while satellite males and female mimics must intrude into territories in order …

2c Dna Content Values In Amaranthus (Amaranthaceae) [Abstract], Donald B. Pratt, Shalini N. Jhangiani, Robert J. Wiggers

Faculty Publications

No abstract provided.

Development And Characterization Of Microsatellite Markers For The Grain Amaranths [Abstract], Melanie A. Mallory, Rozaura V. Hall, Andrea R. Mcnabb, Donald B. Pratt, Eric N. Jellen, Peter J. Maughan

Faculty Publications

No abstract provided.

The Ecology Of Fear: Colonization And Oviposition In Aquatic Systems [Poster], Leeanna Pletcher, Johanna Kraus, James R. Vonesh

Biology Presentations

Introduction

Amphibians and aquatic invertebrates have complex life histories that link aquatic and terrestrial food webs. It has been suggested that amphibian reproduction is an important source of carbon to some aquatic systems. Movement of organisms across the aquatic-terrestrial habitat boundary can represent important subsidies to the receiving habitat. Subsidies are organisms, nutrients, or detritus that cross habitat boundaries and are consumed, and these allochthonous inputs can affect food web structure. Predators can alter subsidies by consuming organisms that would otherwise move across habitat boundaries. Predator induced shifts in habitat selection are a well known non-lethal effect in aquatic systems. …

Mitochondria In Energy-Limited States: Mechanisms That Blunt The Signaling Of Cell Death, Steven C. Hand, Michael A. Menze

Faculty Research & Creative Activity

Cellular conditions experienced during energy-limited states – elevated calcium, shifts in cellular adenylate status, compromised mitochondrial membrane potential – are precisely those that trigger, at least in mammals, the mitochondrion to initiate opening of the permeability transition pore, to assemble additional protein release channels, and to release pro-apoptotic factors. These proapototic factors in turn activate initiator and executer caspases. How is activation of mitochondria-based pathways for the signaling of apoptotic and necrotic cell death avoided under conditions of hypoxia, anoxia, diapause, estivation and anhydrobiosis? Functional trade-offs in environmental tolerance may have occurred in parallel with the evolution of diversified pathways …

Clapper Rails As Indicators Of Mercury And Pcb Bioavailability In A Georgia Saltmarsh System, James C. Cumbee Jr., Karen F. Gaines, Gary L. Mills, N. Garvin, Warren L. Stephens Jr., James N. Novak, I. L. Brisbin Jr.

Faculty Research & Creative Activity

Clapper rails (Rallus longirostris) were used as an indicator species of estuarine marsh habitat quality because of their strong site fidelity and predictable diet consisting of mostly benthic organisms. Mercury (Hg) and the polychlorinated biphenyl (PCB) Aroclor 1268 concentrations were determined for sediments, crabs, as well as clapper rail adults and chicks collected from salt marshes associated with the LCP Superfund site in Brunswick, Georgia. Home ranges were established for adult rails, and sediment and crab samples were taken from each individual’s range. The study was designed to minimize the spatial variability associated with trophic transfer studies by choosing an …

Exposure And Exposure Modeling, Karen F. Gaines, T. E. Chow, S. A. Dyer

Faculty Research & Creative Activity

Exposure to contaminants in the environment is quantified through the ecological risk assessment (ERA) process which provides a framework for the development and implementation of environmental management decisions. The ERA uses available toxicological and ecological information to estimate the probability of occurrence for a specified undesired ecological event or endpoint. The level for these endpoints depends on the objectives and the constraints imposed upon the risk assessment process; therefore, multiple endpoints at different scales may be necessary. ERAs Ecotoxicology | Exposure and Exposure Assessment 1527Author's personal copy often rely on the link between these undesired endpoints to a threshold of …

The Correct Genus For The Jingle Bell Orchid, Harrisella Porrecta, Barbara S. Carlsward, Mark Whitten

Faculty Research & Creative Activity

No abstract provided.

Spatial And Temporal Expression Profiling Of Cell-Wall Invertase Genes During Early Development In Hybrid Poplar, Thomas Canam, Sarah Mak, Shawn Mansfield

Faculty Research & Creative Activity

Cell-wall invertase genes are spatially and temporally regulated in several plant species, including Daucus carota L., Lycopersicon esculentum L. and Solanum tuberosum L. However, few studies of cell-wall invertase genes of trees have been conducted, despite the importance of trees as a source of lignocellulosic biopolymers.We identified three putative cell-wall invertase genes in hybrid poplar (Populus alba L. × grandidentata Michx.) that showed higher homology to each other than to cell-wall invertases of other dicotyledonous species, with two of the genes (Pa×gINV2 and Pa×gINV3) appearing as a genomic tandem repeat. These genes are more similar to each other than to …

Vegetative Anatomy Of Calypsoeae (Orchidaceae), William Stern, Barbara Carlsward

Faculty Research & Creative Activity

Calypsoeae represent a small tribe of anatomically little-known orchids with a wide distribution in the Western Hemisphere. Leaves are present in all genera, except Corallorhiza and Wullschlaegelia both of which are subterranean taxa. Stomata are abaxial (ad- and abaxial in Aplectrum) and tetracytic (anomocytic in Calypso). Fiber bundles are absent in leaves of all taxa examined except Govenia tingens. Stegmata are present in leaves of only Cremastra and Govenia. Roots are velamentous, except in filiform roots of Wullschlaegelia. Vegetative anatomy supports a relationship between Wullschlaegelia and Corallorhiza but does not support the grouping of winter-leaved Aplectrum and Tipularia nor proposed …

The Correct Genus For The Jingle Bell Orchid, Harrisella Porrecta, Barbara Carlsward, Mark Whitten

Faculty Research & Creative Activity

No abstract provided.