Biology Commons^™

Tree Calls Of Three Treefrogs (Hyla Femoralis, H. Gratiosa, And H. Squirella): Analysis Of Environmental, Behavioral, And Acoustic Characteristics, Kristine Schad

Electronic Theses and Dissertations

Male frogs typically call near water at dusk to attract females for breeding. During the breeding season, male treefrogs also emit diurnal "tree calls" or "rain calls" from the tops of trees. Very little is known about tree calls, although many treefrogs use them. Tree calls may be used to attract females, deter males or be triggered by weather conditions favorable for breeding: high temperature and relative humidity, and a drop in barometric pressure. As dusk approaches, male treefrogs continue tree calls from lower in the trees, and if conditions are favorable, jump to the ground and travel to a …

Dynamics Of Cicrobial Communities Associated With Typha Angustifolia (Cattail) Litter In A Wetland Ecosystem: Effects Of Litter Manipulations On Microbial Decay Processes, Andrew John Foley

Master's Theses and Doctoral Dissertations

Quantification of microbial processes involved in plant decomposition is important in understanding energy flow and nutrient recycling in wetlands. Prior studies examining emergent plant decomposition have often used litter that had been prematurely harvested (senescent) and/or manipulated (oven-dried). I examined the effects of litter manipulations on microbial decay dynamics associated with litter of the emergent macrophyte Typha angustifolia. Plant litter was harvested after senescence and after a period of standing-dead decay. This collected litter was either air-dried or oven-dried, placed into fine mesh (1 mm) litterbags, and submerged in the wetland. Litterbags were retrieved periodically and analyzed for microbial biomass, …

Life Without Water : Expression Of Plant Lea Genes By An Anhydrobiotic Arthropod., Steven Hand, Dana Jones, Michael Menze, Trudy Witt

Faculty and Staff Scholarship

Anhydrobiotic animals protect cellular architecture and metabolic machinery in the dry state, yet the molecular repertoire supporting this profound dehydration tolerance is not fully understood. For the desiccation-tolerant crustacean, Artemia franciscana, we report differential expression of two distinct mRNAs encoding for proteins that share sequence similarities and structural features with late-embryogenesis abundant (LEA) proteins originally discovered in plants. Bioinformatic analyses support assignment of the LEA proteins from A. franciscana to group 3. This eucoelomate species is the most highly evolved animal for which LEA gene expression has been reported. It is becoming clear that an ensemble of micromolecules and macromolecules …

The Bioaccumulation Of Persistent Organic Pollutants In Marine Species From Irish And Surrounding Waters, Brendan Mchugh

Doctoral

The presence of anthropogenic pollutants throughout all compartments of the marine environment have been of international concern for a number of decades. While a great number of datasets documenting “absolute” concentrations of persistent organic pollutants in a variety of marine biota are available, bioaccumulation, biomagnification and the fate of these compounds in the marine food web or marine ecosystem is often not possible. This thesis reports various analytical methodologies employed to determine levels of a wide scope of persistent organic pollutants in marine biota and traces the flow of carbon and nitrogen isotopes through the marine food web, ultimately applying …

Models Of Density‐Dependent Genic Selection And A New Rock‐Paper‐Scissors Social System, Barry Sinervo, Benoit Heulin, Yann Surget-Groba, Jean Clobert, Donald B. Miles, Ammon Corl, Alexis Chaine, Alison Davis

Biology Faculty Publications

We describe new ESS models of density regulation driven by genic selection to explain the cyclical dynamics of a social system that exhibits a rock-paper-scissors (RPS) set of three alternative strategies. We tracked changes in morph frequency and fitness of Lacerta vivipara and found conspicuous RPS cycles. Morphs of Uta and Lacerta exhibited parallel survival-performance trade-offs. Frequency cycles in both species of lizards are driven by genic selection. In Lacerta, frequency of each allele in adult cohorts had significant impacts on juvenile recruitment, similar to mutualistic, altruistic, and antagonistic relations of RPS alleles in Uta. We constructed evolutionarily stable strategy …

The Cytomegalovirus Homolog Of Interleukin-10 Requires Phosphatidylinositol 3-Kinase Activity For Inhibition Of Cytokine Synthesis In Monocytes, Juliet V. Spencer

Biology Faculty Publications

Human cytomegalovirus (CMV) has evolved numerous strategies for evading host immune defenses, including piracy of cellular cytokines. A viral homolog of interleukin-10, designated cmvIL-10, binds to the cellular IL-10 receptor and effects potent immune suppression. The signaling pathways employed by cmvIL-10 were investigated, and the classic IL-10R/JAK1/Stat3 pathway was found to be activated in monocytes. However, inhibition of JAK1 had little effect on cmvIL-10-mediated suppression of tumor necrosis factor alpha (TNF-α) production. Inhibition of the phosphatidylinositol 3-kinase/Akt pathway had a more significant impact on TNF-α levels but did not completely relieve the immune suppression, demonstrating that cmvIL-10 stimulates multiple signaling …

Strategies For Odor Coding In The Piriform Cortex, Jessica H. Brann, Shari R. Saideman, Matthew T. Valley, Denise Wiedl

Biology: Faculty Publications and Other Works

No abstract provided.

Observations On The Diets Of An Anuran And Eight Lizard Taxa From Sonora, Chihuahua, And Coahuila, Mexico, With Some Notes On Clutch Sizes, Geoffrey R. Smith, J. A. Lemos-Espinal, Christopher B. Dibble, Megan E. Ogle, Joshua J. Schulte, Andrew J. Terlecky, Allison Boyd

Faculty Publications

No abstract provided.

Increasing Serum 25-Hydroxyvitamin D As A Possible Deterrent To The Onset Of Multiple Sclerosis, Nicholas J. Perretti

Seton Hall University Dissertations and Theses (ETDs)

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Sexual Dimorphism, Population Dynamics And Some Aspects Of Life History Of Echiniscus Mauccii (Tardigrada; Heterotardigrada), Colleen R. Mitchell, Frank A. Romano Iii

Research, Publications & Creative Work

A fifteen month study (December 2002 though February 2004) of a meiofaunal community living in moss and lichen from a Pecan tree on the campus of Jacksonville State University reports 9,791 microinvertebrates. Echiniscus mauccii was the most prevalent tardigrade species (1,329 specimens) and was chosen to determine population dynamics and some aspects of their life histories. The average length of all the specimens (adults, juveniles, males, and females) for each month was determined. A plot of all E. mauccii specimens was used to determine the following life stages of this species; juvenile, pre-reproductive, and reproductive. The studied population exhibited relatively …

The Role Of B2-Glycoprotein I In Homeostatis, Frederic Lin

Doctoral

B2-glycoprotein I (B2GPI) is a phospholipid-binding protein of 326 amino acids found in plasma at a concentration of approx. 180 ug/ml. It has a sequence of positively charged amino acids located at the carboxy terminus that mediates anionic phospholipid binding. B2GPI is suspected to have a role in inhibition of thrombosis. This suspicion is reinforced by the observation that B2GPI is the major target for autoantibodies in the antiphospholipid syndrome. However, little is known about circulating levels of the protein in common thrombotic diseases of inflammation. In the first part of this thesis, we developed a sensitive sandwich-direct ELISA for …

Robustness Of Proxy-Based Climate Field Reconstruction Methods, Scott Rutherford, Michael E. Mann, Eugene Wahl, Caspar Ammann

Arts & Sciences Faculty Publications

We present results from continued investigations into the fidelity of covariance-based climate field reconstruction (CFR) approaches used in proxy-based climate reconstruction. Our experiments employ synthetic “pseudoproxy” data derived from simulations of forced climate changes over the past millennium. Using networks of these pseudoproxy data, we investigate the sensitivity of CFR performance to signal-to-noise ratios, the noise spectrum, the spatial sampling of pseudoproxy locations, the statistical representation of predictors used, and the diagnostic used to quantify reconstruction skill. Our results reinforce previous conclusions that CFR methods, correctly implemented and applied to suitable networks of proxy data, should yield reliable reconstructions of …

Symposium: Effects Of Human Choices On Characteristics Of Urban Ecosystems, Loren Byrne

Arts & Sciences Faculty Publications

Most urban ecology in cities remains an “ecology in cities” rather than an “ecology of cities.” Accomplishing the latter requires the inclusion of humans within the concept of “ecosystem,” both how humans alter the properties of urban ecosystems and how these alterations in turn influence human well-being. These influences are both direct (e.g., physiological and psychological influences on the human organism) and indirect, by influencing ecosystem sustainability.

For the 2007 ESA meeting, Larry Baker, Loren Byrne, Jason Walker, and Alex Felson organized a symposium to address the relationships among human choices and urban ecosystems. In the introductory talk of this …

Functional Characteristics Of Nematocysts Found On The Scyphomedusa Cyanea Capillata, Sean Colin, John H. Costello

Arts & Sciences Faculty Publications

Although prey capture by cnidarians is mediated through nematocysts, their influence on prey selection by cnidarians remains poorly documented. The difficulty in visualizing nematocyst–prey interactions remains the chief obstacle to understanding how the wide variety of nematocyst types influences the mechanics of prey capture. One solution to this limitation has been to assign functional roles to nematocysts based on morphological characters of discharged cnidae. Here we report results of an alternative approach based upon dynamic traits of nematocyst discharge. We examined tubule lengths, tubule discharge velocities and net-to-gross displacement ratios of tubules of discharging nematocysts possessed by the cosmopolitan scyphomedusa, …

Lysmata Rafa, A New Species Of Peppermint Shrimp (Crustacea, Caridea, Hippolytidae) From The Subtropical Western Atlantic, Andrew L. Rhyne, Arthur Anker

Arts & Sciences Faculty Publications

Lysmata rafa n. sp. is described from freshly collected specimens from the Keys West Lakes, Florida Keys, and from a museum specimen collected at Bear Cut, Biscayne Bay, Florida. The new species is morphologically most similar to the western Atlantic Lysmata rathbunae Chace, 1970 and the eastern PaciWc Lysmata gracilirostris Wicksten, 2000, but can be distinguished from them by the number of carpal segments in the second pereiopod; the length and dentition of the rostrum; the shape and number of spines on the dactylus of the third to Wfth pereiopods; and the absence of a tooth on the pterygostomial margin …

Density-Dependent Effect On Reproductive Behaviour Of Lysmata Amboinensis And L. Boggessi (Decapoda: Caridea: Hippolytidae), Dong Zhang, Andrew L. Rhyne, Junda Lin

Arts & Sciences Faculty Publications

We compared the reproductive behaviours of two protandric simultaneous hermaphroditic species (Lysmata amboinensis and L. boggessi) that belong to two groups of Lysmata shrimp with different morphology, geographical distribution, and density. Lysmata amboinensis occurs in tropical waters at low population densities, and L. boggessi is found in aggregation in sub-tropical and temperate areas. Reproductive behaviour of L. boggessi under two densities and L. amboinensis in different habitats were compared. Results show that L. amboinensis was much less active during mating than L. boggessi. Male shrimp of L. amboinensis did not display obvious pre-copulation behaviour. They also took …

Screening New Cytokinesis Genes And Investigation Of Regulation Of Hof1 In Cytokinesis, Jung Eun Park

Masters Theses

"In 1996, a complete sequence of the budding yeast genome was completed and numerous studies begun to discover the function of genes. A primary concern of this study was to identify genes that may function in cytokinesis. Seven candidate genes were chosen, which were previously shown to localize to the bud neck, the site of cytokinesis, or interact with a protein that is involved in cytokinesis. Six of those uncharacterized genes, YHR149C, YLR187W, YOL070C, YMR124W, YPL158C, and YOR304C-A, were identified by data generated by GFP tagging of the yeast proteome. Data from a large-scale two-hybrid screen was used to identify …

Ambystoma: Perspectives On Adaptation And The Evolution Of Vertebrate Genomes, Jeramiah James Smith

University of Kentucky Doctoral Dissertations

Tiger salamanders, and especially the Mexican axolotl (Ambystoma mexicanum), are important model organisms in biological research. This dissertation describes new genomic resources and scientific results that greatly extend the utility of tiger salamanders. With respect to new resources, this dissertation describes the development of expressed sequence tags and assembled contigs, a comparative genome map, a web-portal that makes genomic information freely available to the scientific community, and a computer program that compares structure features of organism genomes. With respect to new scientific results, this dissertation describes a quantitative trait locus that is associated with ecologically and evolutionarily relevant …

Blood, Serum Glucose And Renal Parameters In Lead-Loaded Albino Rats And Treatment With Some Chelating Agents And Natural Oils, Abd El Rahiem A. Ashour, Maged M. Yassin, Nahed M. Abu Aasi, Rokaya M. Ali

Turkish Journal of Biology

This study investigated lead impact on blood, serum glucose and kidney function and the efficacy of treatment with chelating agents meso-2,3-dimercaptosuccinic acid (DMSA), calcium disodium ethylenediaminetetraacetic acid (CaNa_2-EDTA) and natural olive and garlic oils to reduce lead toxicity in albino rats. Oral administration of 1000 or 2000 ppm lead acetate significantly decreased red blood cell count, hemoglobin level and hematocrit value at 20, 40 and 60 days compared with control groups (rats administered deionized water and 1000 or 2000 ppm sodium acetate). Serum glucose was also decreased significantly with both doses of lead acetate administration. In contrast, serum urea, uric …

In Vitro Antimicrobial Activity And Phytochemical Analysis Of Some Indian Medicinal Plants, Jigna Parekh, Sumitra Chanda

Turkish Journal of Biology

The antibacterial effect of some selected Indian medicinal plants was evaluated on bacterial strains like Bacillus cereus ATCC11778, Staphylococcus aureus ATCC25923, Enterobacter aerogenes ATCC13048, Escherichia coli ATCC25922 and Klebsiella pneumoniae NCIM2719. The solvents used for the extraction of plants were water and methanol. The in vitro antibacterial activity was performed by agar disc diffusion and agar well diffusion method. The most susceptible Gram-positive bacteria was B. cereus, while the most susceptible Gram-negative bacteria was K. pneumoniae. The extracts of Abrus precatorius, Cardiospermum halicacabum and Gmelina asiatica could not inhibit any of the bacterial strains investigated. The most active antibacterial plant …

Effect Of 28-Homobrassinolide On Salinity-Induced Changes In Brassica Juncea, Shamsul Hayat, Barket Ali, Syed Aiman Hasan, Aqil Ahmad

Turkish Journal of Biology

Seedlings of Brassica juncea Czern & Coss cv. Varuna generated from seeds soaked in 0, 50, 100, or 150 mM NaCl for 6 h were treated with 0, 10^{-10}, 10^{-8}, or 10^{-6} M 28-homobrassinolide (HBL) after 14 days of growth. Plants that received only NaCl treatment exhibited a decrease in nitrate reductase and carbonic anhydrase activity, chlorophyll content, and P_N 60 days after sowing (DAS), and decreased seed yield at harvest (140 DAS). Subsequent treatment with HBL significantly increased all of the above parameters. The 10^{-8} M concentration of HBL generated the best response and also overcame the detrimental effects …

The Abundance Of Arbuscular Mycorrhizal Fungi Spores In Rhizospheres Of Different Crops, Ali Akbar Safari Sinegani, Zahed Sharifi

Turkish Journal of Biology

The number of spores of vesicular-arbuscular mycorrhizal (VAM) fungi occurring in the rhizospheric soil of 14 crops was investigated using traditional wet-sieving/decanting methods. The aim of the study was to determine the effect of some agronomy species (Gramineae, Leguminosae, Solanaceae, Labiatae, Cruciferae, Umbellifera, and Alliaceae) on VAM spores numbers in the rhizosphere. The agronomy species were planted under greenhouse conditions in pots containing semiarid soil. The number of spores in the rhizosphere of each species was studied at the middle and end of vegetative plant growth. In the middle of vegetative growth the number of spores increased significantly in the …

Planktonic Diatom (Bacillariophyta) Composition Of Lake Kaz (Pazar, Tokat), Esra Zai̇m

Turkish Journal of Biology

The composition, density, and seasonal variations of the planktonic diatoms of Lake Kaz were studied using samples taken from the surface and 1.0 m depths between May 2005 and April 2006. Some physical and chemical properties of the lake water were also determined. Planktonic algae communities were identified, with 143 belonging to Bacillariophyta. Aulocoseira granulata, Aulocoseira italica, Cocconeis placentula, Cocconeis placentula var. euglypta, Cyclotella meneghiniana, Navicula radiosa, and Synedra ulna from the Bacillariophtya were dominant. Seasonal variations in the planktonic flora were observed during that period. According to the chemical and physical analyses performed, the lake has low alkali soft …

Foraging Ecology Of Black-Crowned Night-Herons (Nycticorax Nycticorax) In The New York City Area, Andrew James Bernick

Dissertations, Theses, and Capstone Projects

Over 1,700 pairs of colonial wading birds (i.e., herons, egrets, and ibis) breed and forage in the industrialized ecosystem of metropolitan New York City (NYC). Wading bird colonies are located on 7 islands that lie between western Staten Island and Long Island Sound. The Black-crowned Night-Heron (BCNH), a mainly nocturnal forager, is the numerically dominant breeding heron in these colonies, and has been undergoing population declines both locally and region-wide since the mid-1990s. My objective was to determine how BCNHs forage in NYC's urban estuarine, freshwater, and terrestrial environments. From March-September 2002-2004, I conducted weekly night surveys on Staten Island, …

Growth Of Mc3t3-El Pre-Osteoblasts On Borate Containing Glasses & Growth Of Osteoblastic Cells On 13-93 Glass Scaffolds, Agatha Dwilewicz

Opportunities for Undergraduate Research Experience Program (OURE)

Three borate-based glasses, compositions designated 1B, 2B, and 3B, with B₂0₃ levels of 15, 31, and 46 mole percent (Table 1), respectively, were tested for their effects on the growth of MC3T3-El mouse pre-osteoblastic cells under physiological fluid conditions. Silicate type 45S5 glass was used as a control for comparison. Independently, the biocompatibility of recently developed silicate-based porous 13-93 bioactive glass scaffolds was tested; Saos-2 human osteosarcoma cell line was used. Tests performed for the two studies included: contact assays of cell growth at the glass interface, chemical analysis of borate released into the culture medium, quantitative …

Cell Cycle Analysis Of Atrazine-Treated Human Fibroblast Cells Using Flow Cytometry, Andrea L. Austin

Honors Program Theses

Atrazine is the most frequently used triazine herbicide in the United States. Studies have shown an association between herbicide exposure and increased levels of DNA damage, reproductive and endocrine problems, and an increased risk for certain cancers. The United States Environmental Protection Agency (USEP A) has set a maximum allowable limit at 3 parts per billion (ppb ). Previous work from our lab has shown decreased cell proliferation of normal human fibroblasts after low-level atrazine exposure for 72 hours at 0.8-100 ppb without a corresponding increase in apoptosis or necrosis.

The objective of the current study is to determine a …

Examination Of Post-Translational Modifications And Associate Proteins Of Pcbp1 In Neuronal Cells, Ying-Chih Lin

Seton Hall University Dissertations and Theses (ETDs)

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Germ-Line Chimerism And Paternal Care In Marmosets (Callithrix Kuhlii), Corinna N. Ross, J. A. French, G. Ortí

Biology Faculty Publications (Archived)

The formation of viable genetic chimeras in mammals through the transfer of cells between siblings in utero is rare. Using microsatellite DNA markers, we show here that chimerism in marmoset (Callithrix kuhlii) twins is not limited to blood-derived hematopoietic tissues as was previously described. All somatic tissue types sampled were found to be chimeric. Notably, chimerism was demonstrated to be present in germ-line tissues, an event never before documented as naturally occurring in a primate. In fact, we found that chimeric marmosets often transmit sibling alleles acquired in utero to their own offspring. Thus, an individual that contributes gametes to …

Mda-7/Il-24, Novel Anticancer Cytokine: Focus On Bystander Antitumor, Radiosensitization And Antiangiogenic Properties And Overview Of The Phase I Clinical Experience (Review), Irina V. Lebedeva, Luni Emdad, Zao-Zhong Su, Pankaj Gupta, Moira Sauane, Devanand Sarkar, Michelle R. Staudt, Shi-Jian Liu, Mohiuddin M. Taher, Ruoyu Xiao, Paola Barral, Seok-Geun Lee, Dongning Wang, Nicollaq Vozhilla, Eun Sook Park, Lejuan Chatman, Habib Boukerche, Rajagopal Ramesh, Satoshi Inoue, Sunil Chada, Rong Li, Anthony L. De Pass, Parameshwar J. Mahasreshti, Igor P. Dmitriev, David T. Curiel, Adly Yacoub, Steven Grant, Paul Dent, Neil Senzer, John J. Nemunaitis, Paul B. Fisher

Publications and Research

Subtraction hybridization applied to a ‘differentiation therapy’ model of cancer employing human melanoma cells resulted in the cloning of melanoma differentiation associated gene-7/interleukin-24 (mda-7/IL-24). Initial studies confirm an inverse correlation between mda-7 expression and melanoma development and progression. Forced expression of mda-7 by means of a plasmid or via a replication incompetent adenovirus (Ad.mda-7) promotes growth suppression and induces apoptosis in a broad array of human cancers. In contrast, mda-7 does not induce growth suppressive or toxic effects in normal cells. Based on structure (containing an IL-10 signature motif), secretion by cells (including subsets of T-cells) and location on chromosome …

Pathways Of Anaerobic Carbon Cycling Across An Ombrotrophic–Minerotrophic Peatland Gradient, Jason K. Keller, Scott D. Bridgham

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Peatland soils represent globally significant stores of carbon, and understanding carbon cycling pathways in these ecosystems has important implications for global climate change. We measured aceticlastic and autotrophic methanogenesis, sulfate reduction, denitrification, and iron reduction in a bog, an intermediate fen, and a rich fen in the Upper Peninsula of Michigan for one growing season. In 3-d anaerobic incubations of slurried peat, denitrification and iron reduction were minor components of anaerobic carbon mineralization. Experiments using 14C-labeled methanogenic substrates showed that methanogenesis in these peatlands was primarily through the aceticlastic pathway, except early in the growing season in more ombrotrophic peatlands, …