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Articles 61 - 90 of 303
Full-Text Articles in Biology
Establishing A Direct Role For The Bartonella Bacilliformis Invasion-Associated Locus B (Ialb) Protein In Human Erythrocyte Parasitism, Sherry A. Coleman, Michael F. Minnick
Establishing A Direct Role For The Bartonella Bacilliformis Invasion-Associated Locus B (Ialb) Protein In Human Erythrocyte Parasitism, Sherry A. Coleman, Michael F. Minnick
Biological Sciences Faculty Publications
The invasion-associated locus A and B genes (ialAB) of Bartonella bacilliformis were previously shown to confer an erythrocyte-invasive phenotype upon Escherichia coli, indirectly implicating their role in virulence. We report the first direct demonstration of a role for ialB as a virulence factor in B, bacilliformis. The presence of a secretory signal sequence and amino acid sequence similarity to two known outer membrane proteins involved in virulence suggested that IalB was an outer membrane protein, To develop an antiserum for protein localization, the ialB gene was cloned in frame into an expression vector with a six-histidine tag and under control …
Preparations For Transformation Of Drosophila Melanogaster With Green Fluorescent Protein-Tagged Dmnopp140 And Dmnopp140-Rgg By P-Element Transformation, Jennifer Mccain
Preparations For Transformation Of Drosophila Melanogaster With Green Fluorescent Protein-Tagged Dmnopp140 And Dmnopp140-Rgg By P-Element Transformation, Jennifer Mccain
Honors Capstones
No abstract provided.
Temperature Effects On The Dynamics Of Aedes Albopictus (Diptera : Culicidae) Populations In The Laboratory, Barry W. Alto, Steven A. Juliano
Temperature Effects On The Dynamics Of Aedes Albopictus (Diptera : Culicidae) Populations In The Laboratory, Barry W. Alto, Steven A. Juliano
Faculty Publications – Biological Sciences
We investigated how constant temperatures of 22, 24, and 26 degreesC experienced across the full life cycle affected the dynamics of caged populations of Aedes albopictus (Skuse). All cages were equipped with plastic beakers that served as sites for oviposition and larval development. We measured the per capita daily mortality and emergence rates of the adults and size of adult females, and estimated the intrinsic rate of increase (r) and asymptotic density (K) for each adult population. populations at 26 degreesC had greater intrinsic rates of increase and lower asymptotic densities than populations at 22 and 24 degreesC. Populations at …
Assembly Of Reca-Like Recombinases: Distinct Roles For Mediator Proteins In Mitosis And Meiosis, Stephen L. Gasior
Assembly Of Reca-Like Recombinases: Distinct Roles For Mediator Proteins In Mitosis And Meiosis, Stephen L. Gasior
Biological Sciences Faculty Publications
Members of the RecA family of recombinases from bacteriophage T4, Escherichia coli, yeast, and higher eukaryotes function in recombination as higher-order oligomers assembled on tracts of single-strand DNA (ssDNA). Biochemical studies have shown that assembly of recombinase involves accessory factors. These studies have identified a class of proteins, called recombination mediator proteins, that act by promoting assembly of recombinase on ssDNA tracts that are bound by ssDNA-binding protein (ssb). In the absence of mediators, ssb inhibits recombination reactions by competing with recombinase for DNA-binding sites. Here we briefly review mediated recombinase assembly and present results of new in vivo experiments. …
Comparison Of A Space Shuttle Flight (Sts-78) And Bed Rest On Human Muscle Function, Scott W. Trappe, Todd A. Trappe, Gary A. Lee, Jeffrey J. Widrick, David L. Costill, Robert H. Fitts
Comparison Of A Space Shuttle Flight (Sts-78) And Bed Rest On Human Muscle Function, Scott W. Trappe, Todd A. Trappe, Gary A. Lee, Jeffrey J. Widrick, David L. Costill, Robert H. Fitts
Biological Sciences Faculty Research and Publications
The purpose of this investigation was to assess muscle fiber size, composition, and in vivo contractile characteristics of the calf muscle of four male crew members during a 17-day spaceflight (SF; Life and Microgravity Sciences Spacelab Shuttle Transport System-78 mission) and eight men during a 17-day bed rest (BR). The protocols and timelines of these two investigations were identical, therefore allowing for direct comparisons between SF and the BR. The subjects’ age, height, and weight were 43 +/- 2 yr, 183 +/- 4 cm, and 86 +/- 3 kg for SF and 43 +/- 2 yr, 182 +/- 3 cm, …
Molecular Diversity, Structure And Domestication Of Grasses, Edward S. Buckler Iv, Jeffry M. Thornsberry, Stephen Kresovich
Molecular Diversity, Structure And Domestication Of Grasses, Edward S. Buckler Iv, Jeffry M. Thornsberry, Stephen Kresovich
Faculty Publications
Over the last 10,000 years, crop domestication has been the single most important human cultural development. Grasses are prominent among these crops, and provide the vast majority of the world's food. Similar traits have been selected during the domestication and breeding of these critically important grasses, and since they share a similar complement of genes, the same set of genes may have been selected. Even though the process of domestication occurred over the same 5000 to 10,000 year period, the domesticated grasses have major differences in genome structure, diversity, and life history. Molecular investigations of grass domestication have succeeded in …
Different Regulatory Mechanisms Modulate The Expression Of A Dinoflagellate Iron-Superoxide Dismutase, O. Keith Okamoto, Deborah L. Robertson, Thomas F. Fagan, J. Woodland Hastings, Pio Colepicolo
Different Regulatory Mechanisms Modulate The Expression Of A Dinoflagellate Iron-Superoxide Dismutase, O. Keith Okamoto, Deborah L. Robertson, Thomas F. Fagan, J. Woodland Hastings, Pio Colepicolo
Biology
Regulation of antioxidant enzymes is critical to control the levels of reactive oxygen species in cell compartments highly susceptible to oxidative stress. In this work, we studied the regulation of a chloroplastic iron superoxide dismutase (Fe-SOD) from Lingulodinium polyedrum (formerly Gonyaulax polyedra) under different physiological conditions. A cDNA-encoding Fe-SOD was isolated from this dinoflagellate, showing high sequence similarity to cyanobacterial, algal, and plant Fe-SODs. Under standard growth conditions, on a 12:12-h light-dark cycle, Lingulodinium polyedrum Fe-SOD exhibited a daily rhythm of activity and cellular abundance with the maximum occurring during the middle of the light phase. Northern analyses showed that …
G Protein Regulation Of Ion Channels And Abscisic Acid Signaling In Arabidopsis Guard Cells, Hemayet Ullah, Xi-Qing Wang, Alan Jones, Sarah Assmann
G Protein Regulation Of Ion Channels And Abscisic Acid Signaling In Arabidopsis Guard Cells, Hemayet Ullah, Xi-Qing Wang, Alan Jones, Sarah Assmann
Department of Biology Faculty Publications
The phytohormone abscisic acid (ABA) promotes plant water conservation by decreasing the apertures of stomatal pores in the epidermis through which water loss occurs. We found that Arabidopsis thaliana plants harboring transferred DNA insertional mutations in the sole prototypical heterotrimeric GTP-binding (G) protein alpha subunit gene, GPA1, lack both ABA inhibition of guard cell inward K(+) channels and pH-independent ABA activation of anion channels. Stomatal opening in gpa1 plants is insensitive to inhibition by ABA, and the rate of water loss from gpa1 mutants is greater than that from wild-type plants. Manipulation of G protein status in guard cells may …
Parasitism Patterns And The Size-Fecundity Relationship In The Acoustically Orienting Dipteran Parasitoid Ormia Ochracea, Gita R. Kolluru, Marlene Zuk
Parasitism Patterns And The Size-Fecundity Relationship In The Acoustically Orienting Dipteran Parasitoid Ormia Ochracea, Gita R. Kolluru, Marlene Zuk
Biological Sciences
Female parasitoids are expected to distribute offspring among hosts in a manner that maximizes fitness. Several theoretical and empirical studies have focussed on clutch-size patterns in hymenopteran parasitoids. In contrast, dipteran parasitoids, which differ from hymenopterans in potentially important ways, have received little attention. The phonotactic tachinid fly Ormia ochracea has been intensively studied for its effects on host crickets, and has recently been the subject of studies of its own reproductive biology. This work suggests a negative relationship between clutch size and progeny fitness (consistent with hymenopterans), but no adjustment of clutch size to host size (different from hymenopterans). …
Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric K. Bollinger
Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric K. Bollinger
Faculty Research & Creative Activity
This book review appeared in The Auk, Volume 118 No. 3 (July 2001).
Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric Bollinger
Book Review Of "Ecology And Conservation Of Grassland Birds Of The Western Hemisphere", Eric Bollinger
Faculty Research & Creative Activity
This book review appeared in The Auk, Volume 118 No. 3 (July 2001).
Invasion Genetics Of New World Medflies: Testing Alternative Colonization Scenarios, Andrew J. Bohonak, Neil Davies, Francis X. Villablanca, George K. Roderick
Invasion Genetics Of New World Medflies: Testing Alternative Colonization Scenarios, Andrew J. Bohonak, Neil Davies, Francis X. Villablanca, George K. Roderick
Biological Sciences
The Mediterranean fruit fly (Ceratitis capitata) is an invasive agricultural pest with a wide host range and a nearly global distribution. Efforts to forgo the medfly''s spread into the United States are dependent on an understanding of population dynamics in newly established populations elsewhere. To explore the potential influence of demographic and historical parameters in six medfly populations distributed from Mexico to Peru, we created population genetic null models using Monte Carlo simulations. Null expectations for genetic differentiation (F ST) were compared with actual sequence variation from four highly polymorphic nuclear loci. Four colonization scenarios that …
Modulation Of Cell Proliferation By Heterotrimeric G Protein In Arabidopsis, Hemayet Ullah
Modulation Of Cell Proliferation By Heterotrimeric G Protein In Arabidopsis, Hemayet Ullah
Department of Biology Faculty Publications
The alpha subunit of a prototypical heterotrimeric GTP-binding protein (G protein), which is encoded by a single gene (GPA1) in Arabidopsis, is a modulator of plant cell proliferation. gpa1 null mutants have reduced cell division in aerial tissues throughout development. Inducible overexpression of GPA1 in Arabidopsis confers inducible ectopic cell division. GPA1 overexpression in synchronized BY-2 cells causes premature advance of the nuclear cycle and the premature appearance of a division wall. Results from loss of function and ectopic expression and activation of GPA1 indicate that this subunit is a positive modulator of cell division in plants.
Characterization Of Partially Transesterified Poly(Β-Hydroxyalkanoate)S By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry, Broderick Eribo
Characterization Of Partially Transesterified Poly(Β-Hydroxyalkanoate)S By Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry, Broderick Eribo
Department of Biology Faculty Publications
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for the characterization of a partially transesterified poly(beta-hydroxyalkanoate) (PHA), a polymer produced by the bacterial strain Alcaligenes eutrophus with saponified vegetable oils as the sole carbon sources. The transesterification was carried out separately under acidic and basic conditions to obtain PHA oligomers weighing <10 kDa. The intact oligomers were detected in their cationized forms, [M + Na]+ and [M + K]+, by MALDI-TOFMS. A composition analysis, using the MALDI-TOF spectra, indicated that the oligomers obtained via acid catalysis contained a methyl 3-hydroxybutyrate end group, and those obtained by base catalysis had a methyl crotonate (olefinic) end group. In addition to hydroxybutyrate (HB), the oligomers were found to contain a small percentage of hydroxyvalerate, which was independently confirmed by gas chromatography/mass spectrometry. In comparison, analysis of a commercial PHA polymer, transesterified under identical conditions, showed only the presence of HB, i.e., a pure poly(HB) homopolymer.
The Mayfly Newsletter, Peter M. Grant
The Mayfly Newsletter, Peter M. Grant
The Mayfly Newsletter
The Mayfly Newsletter is the official newsletter of the Permanent Committee of the International Conferences on Ephemeroptera.
High Species Diversity In Fleshy-Fruited Tropical Understory Plants, James F. Smith
High Species Diversity In Fleshy-Fruited Tropical Understory Plants, James F. Smith
Biology Faculty Publications and Presentations
Key innovations may increase the number of taxa in a clade that possesses the proposed innovation in comparison to its sister group that lacks the trait through either increased speciation or reduced extinction rates. Comparing sister clades across several independent lineages provides statistical support that the trait has increased species diversity. Previous studies have indicated that there may not be a relationship between biotic dispersal and higher species diversity, but only a few of these studies specified habit, habitat, or type of disperser. No previous study has specified all of the above parameters and used a phylogenetic approach. This article …
Combined, Functional Genomic-Biochemical Approach To Intermediary Metabolism: Interaction Of Acivicin, A Glutamine Amidotransferase Inhibitor, With Escherichia Coli K-12, D. R. Smulski, L. L. Huang, M. P. Mccluskey, M. J. Gladnick Reeve, Amy Cheng Vollmer, T. K. Van Dyk, R. A. Larossa
Combined, Functional Genomic-Biochemical Approach To Intermediary Metabolism: Interaction Of Acivicin, A Glutamine Amidotransferase Inhibitor, With Escherichia Coli K-12, D. R. Smulski, L. L. Huang, M. P. Mccluskey, M. J. Gladnick Reeve, Amy Cheng Vollmer, T. K. Van Dyk, R. A. Larossa
Biology Faculty Works
Acivicin, a modified amino acid natural product, is a glutamine analog. Thus, it might interfere with metabolism by hindering glutamine transport, formation, or usage in processes such as transamidation and translation. This molecule prevented the growth of Escherichia coli in minimal medium unless the medium was supplemented with a purine or histidine, suggesting that the HisHF enzyme, a glutamine amidotransferase, was the target of acivicin action. This enzyme, purified from E. coli, was inhibited by low concentrations of acivicin. Acivicin inhibition was overcome by the presence of three distinct genetic regions when harbored on multicopy plasmids. Comprehensive transcript profiling using …
Comparative Phylogeography Of Mesoamerican Highland Rodents: Concerted Versus Independent Response To Past Climatic Fluctuations, Duke S. Rogers, Jack Sullivan, Elizabeth Arellano
Comparative Phylogeography Of Mesoamerican Highland Rodents: Concerted Versus Independent Response To Past Climatic Fluctuations, Duke S. Rogers, Jack Sullivan, Elizabeth Arellano
Faculty Publications
The phylogeography of Sumichrast's harvest mouse (Reithrodontomys sumichrasti) was examined through maximumlikelihood and parsimony analyses of 1,130 bp of mitochondrial Cytochrome b sequence data from 43 individuals. The phylogeography of this Middle American highland forest-dwelling species was compared to that previously published for the codistributed Aztec deer mouse complex (Peromyscus aztecus/Peromyscus hylocetes complex) in order to test competing hypotheses of concerted versus independent responses of codistributed forms to past climatic fluctuations. Qualitatively, there were strong similarities in the phylogeographic patterns of the two groups, yet there were also areas of incongruence. Likelihood-ratio tests (Kishino-Hasegawa-Templeton and parametric bootstrap tests) indicated that …
First Nesting Records Of The Black Phoebe (Sayornis Nigricans) In Southernmost Texas, Timothy Brush
First Nesting Records Of The Black Phoebe (Sayornis Nigricans) In Southernmost Texas, Timothy Brush
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)
No abstract provided.
The Wings Of Bombyx Mori Develop From Larval Discs Exhibiting An Early Differentiated State: A Preliminary Report, Madhuri Kango-Singh, Amit Singh, K. P. Gopinathan
The Wings Of Bombyx Mori Develop From Larval Discs Exhibiting An Early Differentiated State: A Preliminary Report, Madhuri Kango-Singh, Amit Singh, K. P. Gopinathan
Biology Faculty Publications
Lepidopteran insects present a complex organization of appendages which develop by various mechanisms. In the mulberry silkworm, Bombyx mori, a pair of meso- and meta-thoracic discs located on either side in the larvae gives rise to the corresponding fore- and hind-wings of the adult. These discs do not experience massive cell rearrangements during metamorphosis and display the adult wing vein pattern. We have analysed wing development in B. mori by two approaches, viz., expression of patterning genes in larval wing discs, and regulatory capacities of larval discs following explantation or perturbation. Expression of Nubbin is seen all over the presumptive …
Ecology Of Audubon's Shearwaters (Puffinus Lherminieri) At San Salvador, Bahamas, Neville Anthony Trimm Jr.
Ecology Of Audubon's Shearwaters (Puffinus Lherminieri) At San Salvador, Bahamas, Neville Anthony Trimm Jr.
Loma Linda University Electronic Theses, Dissertations & Projects
The Audubon's Shearwater (Pqffinus lherminieri), nearly pantropical in distribution, remains a poorly studied species. The subspecies P. l. lherminieri is endemic to the West Indies region. An estimated 5,000 pairs inhabit the Caribbean, but there has been a substantial decline with many colonies becoming extirpated. It is thought that as many as 3,000 pairs (60% of the West Indian population) nest in the Bahamas. In May and June of 2000, I collected data on shearwater demographics, colony site selection, burrow microhabitat, chick behavior and the effect of moonlight on the onset of calling of the Audubon's Shearwaters nesting on …
Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto
Comparison Of The Quantification Of Caffeine In Human Plasma By Gas Chromatography And Elisa, A. B. Carregaro, W. E. Woods, Thomas Tobin, A. Queiroz-Neto
Veterinary Science Faculty Publications
In the present study we evaluated the precision of the ELISA method to quantify caffeine in human plasma and compared the results with those obtained by gas chromatography. A total of 58 samples were analyzed by gas chromatography using a nitrogen-phosphorus detector and routine techniques. For the ELISA test, the samples were diluted to obtain a concentration corresponding to 50% of the absorbance of the standard curve. To determine whether the proximity between the I50 of the standard curve and that of the sample would bring about a more precise result, the samples were divided into three blocks according …
Salivary Acinar Cells From Aquaporin 5-Deficient Mice Have Decreased Membrane Water Permeability And Altered Cell Volume Regulation, Carissa M. Krane, James E. Melvin, Ha-Van Nguyen, Linda Richardson, Jennifer E. Towne, Thomas Doetschman, Anil G. Menon
Salivary Acinar Cells From Aquaporin 5-Deficient Mice Have Decreased Membrane Water Permeability And Altered Cell Volume Regulation, Carissa M. Krane, James E. Melvin, Ha-Van Nguyen, Linda Richardson, Jennifer E. Towne, Thomas Doetschman, Anil G. Menon
Biology Faculty Publications
Aquaporins (AQPs) are channel proteins that regulate the movement of water through the plasma membrane of secretory and absorptive cells in response to osmotic gradients. In the salivary gland, AQP5 is the major aquaporin expressed on the apical membrane of acinar cells. Previous studies have shown that the volume of saliva secreted by AQP5-deficient mice is decreased, indicating a role for AQP5 in saliva secretion; however, the mechanism by which AQP5 regulates water transport in salivary acinar cells remains to be determined. Here we show that the decreased salivary flow rate and increased tonicity of the saliva secreted byAqp5 …
A Setback To The Dialogue: Response To Huston Smith, Ursula Goodenough
A Setback To The Dialogue: Response To Huston Smith, Ursula Goodenough
Biology Faculty Research
Huston Smith's book, Why Religion Matters, offers an eloquent evocation of mystical sensibility. Unfortunately, along the way, he offers a strongly negative and often inaccurate account of the scientific worldview, the claim being that the science is laying siege to the spiritual.
Ecology Of The Endangered Sandy Cay Rock Iguana, Cyclura Rileyi Cristata, In The Bahamas, Shawn Kendall Fry
Ecology Of The Endangered Sandy Cay Rock Iguana, Cyclura Rileyi Cristata, In The Bahamas, Shawn Kendall Fry
Loma Linda University Electronic Theses, Dissertations & Projects
In this thesis I present the results of a study on the population demographics and home range size of Cyclura rileyi cristata. There are eight species of West Indian rock iguanas, genus Cyclura. All are endangered, including C. rileyiwhich is endemic to the Bahamas. One of three recognized subspecies, C. r. cristatalives on a single cay in the Exumas chain and is among the most endangered lizards in the world. Various size measurements indicate that this taxon is among the smallest within the genus. Distance surveys and a Lincoln-Petersen estimate based on resightings of marked iguanas …
Functional Properties Of Slow And Fast Gastrocnemius Muscle Fibers After A 17-Day Spaceflight, Jeffrey J. Widrick, Janell Romatowski, Kris M. Norenberg, Shannon T. Knuth, James L. W. Bain, Danny A. Riley, S. W. Trappe, Todd A. Trappe, David Costill, Robert H. Fitts
Functional Properties Of Slow And Fast Gastrocnemius Muscle Fibers After A 17-Day Spaceflight, Jeffrey J. Widrick, Janell Romatowski, Kris M. Norenberg, Shannon T. Knuth, James L. W. Bain, Danny A. Riley, S. W. Trappe, Todd A. Trappe, David Costill, Robert H. Fitts
Biological Sciences Faculty Research and Publications
The purpose of this investigation was to study the effects of a 17-day spaceflight on the contractile properties of individual fast- and slowtwitch fibers isolated from biopsies of the fast-twitch gastrocnemius muscle of four male astronauts. Single chemically skinned fibers were studied during maximal Ca2+-activated contractions with fiber myosin heavy chain (MHC) isoform expression subsequently determined by SDS gel electrophoresis. Spaceflight had no significant effect on the mean diameter or specific force of single fibers expressing type I, IIa, or IIa/IIx MHC, although a small reduction in average absolute force (Po) was observed for the type …
Restoration Of Fish Habitat In The Provo River: A Multispecies Approach, Mark C. Belk
Restoration Of Fish Habitat In The Provo River: A Multispecies Approach, Mark C. Belk
Faculty Publications
Historically, the Provo river, located in Utah and Wasatch Cos., Utah, was a clear-flowing stream with headwaters in the western edge of the Uinta mountains that emptied into Utah Lake near Provo, Utah. The river had a wide flood plain and a braided channel, especially in the Heber valley and Utah valley areas. Diversion of water and construction of water storage facilities began in the late 1800's and intensified in the 1900's. Deer Creek reservoir was built in the 1950's and diking and channelization continued until the construction of Jordanelle reservoir in the late 1980's. The Provo River Restoration Project, …
Analysis And New Records Of Billfish (Teleostei: Perciformers: Istiophoridae) From The Yorktown Formation, Early Pliocene Of Eastern North Carolina At Lee Creek Mine, Harry L. Fierstine
Analysis And New Records Of Billfish (Teleostei: Perciformers: Istiophoridae) From The Yorktown Formation, Early Pliocene Of Eastern North Carolina At Lee Creek Mine, Harry L. Fierstine
Biological Sciences
Five species of the billfish family Istiophoridae (Istiophorus platypterus (Shaw and Nodder), Makaira indica (Cuvier), M. nigricans Lacépède, M. purdyi Fierstine, Tetrapturus albidus Poey) were identified from approximately 500 separate bones collected in the Yorktown Formation (early Pliocene) at Lee Creek Mine, North Carolina. This is the only record of M. purdyi, the first fossil record of the genus Tetrapturus (specifically T. albidus), the second fossil record of I. platypterus and M. indica, and the first record of I. platypterus, M. indica, M. nigricans, and T. albidus from fossil deposits bordering the Atlantic Ocean.
Identification was …
Neuromotor And Cognitive Development In Children With Cerebral Palsy In Relation To Neural Plasticity, Vaniecea Pollard
Neuromotor And Cognitive Development In Children With Cerebral Palsy In Relation To Neural Plasticity, Vaniecea Pollard
McCabe Thesis Collection
Infants with neurological disabilities, such as cerebral palsy (CP) and Down syndrome (DS), show severe delays in motor and cognitive development relative to chronological age. Involvement of neural plasticity as a treatment of such neurological disorders is increasingly being seen. Previous studies have found that interventions involving sensory linked motor performance have been critical in facilitating motor improvement. The purpose of this study was to compare self-initiated mobility in children with CP and DS. Three infants, ages 8, 9, and 22 months, with diagnoses of no CP, DS, and CP respectively, participated in the study. The infants were videotaped in …
16s Ribosomal Dna Terminal Restriction Fragment Pattern Analysis Of Bacterial Communities In Feces Of Rats Fed Lactobacillus Acidophilus Ncfm, Christopher W. Kaplan, Johanna C. Astaire, M. E. Sanders, Bandaru S. Reddy, Christopher L. Kitts
16s Ribosomal Dna Terminal Restriction Fragment Pattern Analysis Of Bacterial Communities In Feces Of Rats Fed Lactobacillus Acidophilus Ncfm, Christopher W. Kaplan, Johanna C. Astaire, M. E. Sanders, Bandaru S. Reddy, Christopher L. Kitts
Biological Sciences
16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics of Lactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence of L. johnsonii was confirmed by isolation from feces.