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Selected Works

2011

Wendy M Schluchter

Articles 1 - 4 of 4

Full-Text Articles in Biology

Characterization Of The Activities Of The Cpey, Cpez, And Cpes Bilin Lyases In Phycoerythrin Biosynthesis In Fremyella Diplosiphon Strain Utex 481*, Wendy Schluchter Oct 2011

Characterization Of The Activities Of The Cpey, Cpez, And Cpes Bilin Lyases In Phycoerythrin Biosynthesis In Fremyella Diplosiphon Strain Utex 481*, Wendy Schluchter

Wendy M Schluchter

When grown in green light, Fremyella diplosiphon strain UTEX481 produces the red-colored protein phycoerythrin (PE) to maximize photosynthetic light harvesting. PE is composed of two subunits, CpeA and CpeB, which carry two and three phycoerythrobilin (PEB) chromophores, respectively, that are attached to specific Cys residues via thioether linkages. Specific bilin lyases are hypothesized to catalyze each PEB ligation. Using a heterologous, coexpression system in Escherichia coli, the PEB ligation activities of putative lyase subunits CpeY, CpeZ, and CpeS were tested on the CpeA and CpeB subunits from F. diplosiphon. Purified His6-tagged CpeA, obtained by coexpressing cpeA, cpeYZ, and the genes …

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Effects Of Modified Phycobilin Biosynthesis In The Cyanobacterium Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter Dec 2010

Effects Of Modified Phycobilin Biosynthesis In The Cyanobacterium Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter

Wendy M Schluchter

No abstract provided.

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Attachment Of Non-Cognate Chromophores To Cpca Of Synechocystis Sp. Strain Pcc 6803 Expressed In E. Coli, Wendy M. Schluchter Dec 2010

Attachment Of Non-Cognate Chromophores To Cpca Of Synechocystis Sp. Strain Pcc 6803 Expressed In E. Coli, Wendy M. Schluchter

Wendy M Schluchter

No abstract provided.

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Biosynthesis Of Cyanobacterial Phycobiliproteins In Escherichia Coli: Chromophorylation Efficiency And Specificity Of All Bilin Lyases From Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter Dec 2010

Biosynthesis Of Cyanobacterial Phycobiliproteins In Escherichia Coli: Chromophorylation Efficiency And Specificity Of All Bilin Lyases From Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter

Wendy M Schluchter

No abstract provided.

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