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- 4 dichlorophenoxyacetic acid 6 n benzyladenine dicamba kinetin article callus (plant) concentration (parameters) controlled study greenhouse Murashige and Skoog medium nonhuman plant development plant growth plant root plantlet process development shoot (1)
- Adventitious buds Banana Micropropagation Musa acuminata benzyladenine cytokinin indoleacetic acid unclassified drug article budding concentration (parameters) controlled study culture medium in vitro propagation nonhuman plant growth plant reproduction plant tissue culture (1)
- Boesenbergia rotunda Callus Plant proteomics Temu kunci Two dimensional electrophoresis Boesenbergia rotunda extract enzyme plant extract protein proteome unclassified drug antibacterial activity antifungal activity antiinflammatory activity antineoplastic activity antioxidant activity antiviral activity article biosynthesis controlled study food in vitro study nonhuman proteomics reproducibility Southeast Asia suspension cell culture traditional medicine two dimensional gel electrophoresis Zingiberaceae (1)
- Boesenbergia rotunda Medicinal plant Micropropagation Organogenesis Plant cell cultures Zingiberaceae 2 (1)
- Boesenbergia rotunda Medicinal plant Micropropagation Zingiberaceae 1 naphthylacetic acid 6 n benzyladenine gelrite sucrose acclimatization article bud concentration (parameters) culture technique inoculation Murashige and Skoog medium nonhuman rhizome rooting shoot (1)
Articles 1 - 5 of 5
Full-Text Articles in Biology
Optimization Of Two-Dimensional Gel Electrophoresis Protocols For Boesenbergia Rotunda In Vitro Suspension Culture, Norzulaani Khalid
Optimization Of Two-Dimensional Gel Electrophoresis Protocols For Boesenbergia Rotunda In Vitro Suspension Culture, Norzulaani Khalid
Norzulaani Khalid
Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures.
Effect Of Benzylaminopurine (Bap) Pulsing On In Vitro Shoot Multiplication Of Musa Acuminata (Banana) Cv. Berangan, Norzulaani Khalid
Effect Of Benzylaminopurine (Bap) Pulsing On In Vitro Shoot Multiplication Of Musa Acuminata (Banana) Cv. Berangan, Norzulaani Khalid
Norzulaani Khalid
MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of abnormal shoots. After the first apical bud appeared, explants were transferred to MS medium with lower concentrations of BAP either with or without indole acetic acid (IAA). Proliferation media supplemented with IAA showed enhanced shoot multiplication and elongation but did not help to reduce the abnormality index that occurred
Rapid Micropropagation Of Boesenbergia Rotunda (L.) Mansf. Kulturpfl. (A Valuable Medicinal Plant) From Shoot Bud Explants, Norzulaani Khalid
Rapid Micropropagation Of Boesenbergia Rotunda (L.) Mansf. Kulturpfl. (A Valuable Medicinal Plant) From Shoot Bud Explants, Norzulaani Khalid
Norzulaani Khalid
A successful protocol was developed for mass propagation of Boesenbergia rotunda (L.) Mansf. Kulturpfl., an important medicinal plant. Numerous shoots were induced from young shoot bud of B. rotunda mature rhizome on Murashige and Skoog (1962) medium supplemented with 30.0 g/l sucrose, 2.0 g/l gelrite, different concentrations of 6-benzylaminopurine (BAP) and α-naphtaleneacetic acid (NAA). Plant medium supplemented with different concentrations of BAP alone or with NAA produced varying degree of multiple shoots. A supplementation of 2.0 mg/l BAP and 0.5 mg/l NAA gave the best result. Ninety percent of the explants induced multiple shoots within 10 to 14 days of …
Determining The Optimal Concentration Of Mannose As An Effective Selection Agent For Transformed Oil Palm Cells Using The Phosphomannose Isomerase (Pmi) Gene As A Positive Selectable Marker, Norzulaani Khalid
Norzulaani Khalid
The elimination of antibiotic or herbicide resistance gene usage in genetically modified plants is being encouraged due to public concern. In response to this, alternative selection systems for the recovery of transgenic oil palm were developed using positive selectable markers. To establish a selection system that utilises the phosphomannose isomerase (pmi) gene for oil palm transformation, we first determined the optimal mannose concentration for selecting the transformed cells. Non-transformed embryogenic calli were cultured on media containing various combinations and concentrations of mannose and a usable source of carbon, i.e. sucrose, ranging in content from 0 to 30 g litre . …
Efficient Propagation Of An Important Medicinal Plant Boesenbergia Rotunda By Shoot Derived Callus, Norzulaani Khalid
Efficient Propagation Of An Important Medicinal Plant Boesenbergia Rotunda By Shoot Derived Callus, Norzulaani Khalid
Norzulaani Khalid
A protocol for high frequency shoot organogenesis and plant establishment from shoot base derived callus was developed for Boesenbergia rotunda (L.) Mansf. Kulturpfl. The cultures produced callus in MS medium supplemented with various concentrations of dicamba and 2,4-D. Nevertheless, embryogenic callus only emerged on 2, 4-D augmented MS medium. In addition, the success of callus induction was achieved from meristem derived callus producing 100% of embryogenic callus. Multiple shoot induction was achieved from the surface of the callus after transferring onto MS medium supplemented with BAP or kinetin (shoot induction medium). The highest frequency of callus producing shoots (49.8%) was …