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Articles 661 - 690 of 1681
Full-Text Articles in Biology
Kremen Is Beyond A Subsidiary Co-Receptor Of Wnt Signaling: An In Silico Validation, Hemn Mohammadpour, Saeed Khalili, Zahra Sadat Hashemi
Kremen Is Beyond A Subsidiary Co-Receptor Of Wnt Signaling: An In Silico Validation, Hemn Mohammadpour, Saeed Khalili, Zahra Sadat Hashemi
Turkish Journal of Biology
Kremen-1 is a co-receptor of Wnt signaling, interacting with the Dkk-3 protein, which is an antagonist of the Wnt/ß catenin pathway. In the present study we attempted to shed some light on the possible orientations of Kremen/Dkk-3 interactions, explaining the mechanisms of Kremen and Dkk-3 functions. Employing state-of-the-art software, a Kremen model was built and subsequently refined. The quality of the final model was evaluated using RMSD calculations. Ultimately, we used the Kremen model for docking analysis between Kremen and Dkk-3 molecules. A model built by the Robetta server showed the best quality scores. Near native coordination of the final …
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Effects Of Turkish Propolis Extract On Secretion Of Polymorphonuclear Elastase Following Respiratory Burst, Yaşam Barlak, Orhan Değer, Meltem Uçar, Tuğba Ni̇gar Çakiroğlu
Turkish Journal of Biology
Propolis is a natural, resinous, hive product with various bioactive properties. Polymorphonuclear leukocytes (PMNLs) use both oxygen-dependent (respiratory burst) and oxygen-independent mechanisms (releasing proteolytic enzymes such as elastase) to destroy infectious agents. The in vitro effects of dimethyl sulfoxide (DMSO) extract of Turkish propolis on the secretion of PMN elastase from PMNLs following respiratory burst was studied. Phorbol myristate acetate was used to stimulate respiratory burst. The Flow Cytometric CellProbe Oxidative Burst Test was used to determinate if respiratory burst occurred or not and what its severity was after a 4 h incubation with propolis extracts (0 mg/mL, 0.625 mg/mL, …
Screening And Characterization Of Biosurfactant-Producing Bacteria Isolatedfrom The Arabian Sea Coast Of Karachi, Erum Shoeb, Nuzhat Ahmed, Jameela Akhter, Uzma Badar, Khaizran Siddiqui, Faiza Ansari, Maheen Waqar, Samina Imtiaz, Nimrah Akhtar, Qurat Ul Ain Shaikh, Roanuq Baig, Saba Butt, Saema Khan, Sidra Khan, Shahid Hussain
Screening And Characterization Of Biosurfactant-Producing Bacteria Isolatedfrom The Arabian Sea Coast Of Karachi, Erum Shoeb, Nuzhat Ahmed, Jameela Akhter, Uzma Badar, Khaizran Siddiqui, Faiza Ansari, Maheen Waqar, Samina Imtiaz, Nimrah Akhtar, Qurat Ul Ain Shaikh, Roanuq Baig, Saba Butt, Saema Khan, Sidra Khan, Shahid Hussain
Turkish Journal of Biology
The aim of the present study was to investigate biosurfactant-producing culturable bacteria inhabiting the coast of the Arabian Sea at Karachi. Overall, 15 seawater samples were collected from the Arabian Sea coast of Karachi. Isolation, characterization, and screening for 89 biosurfactant-producing bacterial strains were conducted through 8 conventional screening tests. Through GSP agar plate method 22 strains were found to be Pseudomonas aeruginosa and Gram reaction revealed 70% of the isolates to be gram-negative. Furthermore, 24% of the isolates showed hemolytic activity, 44% exhibited positive results for oil-spreading test, 54% showed emulsification to at least 1 of the 3 hydrocarbons …
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Ultrastructural Effects Of Lead Acetate On The Spleen Of Rats, Mesure Türkay, Hüseyi̇n Türker, Turan Güven
Turkish Journal of Biology
In this experiment, the ultrastructural effects of orally administered lead acetate on rat (Rattus rattus) spleen were studied. Rats weighing approximately 200-250 g were used in this experiment. They were divided into five groups, each one including five rats. Group I served as the normal control group while the others served as the experimental groups. The rats were exposed to oral administrations of lead acetate of 2.5 mg dissolved in 1 mL of drinking water per day. The administration lasted 96, 144, 336, and 432 h. The animals were anesthetized using chloroform inhalation and the peritoneum was stripped open and …
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Phytofabrication Of Biomolecule-Coated Metallic Silver Nanoparticles Using Leaf Extracts Of In Vitro-Raised Bamboo Species And Its Anticancer Activity Against Human Pc3 Cell Lines, Kalamegam Kalaiarasi, Govindaraj Prasannaraj, Shivendra Vikram Sahi, Perumal Venkatachalam
Turkish Journal of Biology
The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30-90 nm), and EDX analysis. Cell viability assays were carried …
Micropropagation Of Vaccinium Myrtillus L. (Bilberry) Naturally Growingin The Turkish Flora, Mustafa Cüce, Atalay Sökmen
Micropropagation Of Vaccinium Myrtillus L. (Bilberry) Naturally Growingin The Turkish Flora, Mustafa Cüce, Atalay Sökmen
Turkish Journal of Biology
This study was designed to micropropagate Vaccinium myrtillus L., a naturally growing bilberry, in the Turkish flora. In order to determine the most effective basal medium, lateral buds were initially selected as explants, which were then individually cultured in Murashige and Skoog medium, Anderson's rhododendron medium, and McCown's woody plant medium (WPM) supplemented with 1.0 mg L-1 zeatin in combination with either 0.1 mg L-1 indole-3-butyric acid (IBA) or 0.1 mg L-1 \alpha-naphthalene acetic acid. WPM, supplemented with zeatin and IBA, was found to be the most effective basal medium tested. As shoot regeneration ability highly depends on the medium …
Salicylic Acid Alleviates Cold-Induced Photosynthesis Inhibition Andoxidative Stress In Jasminum Sambac, Han Cai, Mengying He, Kun Ma, Yonggao Huang, Yun Wang
Salicylic Acid Alleviates Cold-Induced Photosynthesis Inhibition Andoxidative Stress In Jasminum Sambac, Han Cai, Mengying He, Kun Ma, Yonggao Huang, Yun Wang
Turkish Journal of Biology
Salicylic acid (SA) is a signal molecule that mediates many biotic and abiotic stress-induced physiological responses in plants. In the current study the protective effects of SA on cold stress-caused oxidative damage and photosynthesis inhibition in jasmine plants (Jasminum sambac) were examined. Jasmine seedlings were pretreated with 100 µM SA for 3 days and then subjected to cold stress (4 °C) for 15 days. The amounts of superoxide radicals (O_2^{-}) and hydrogen peroxide (H_{2}O_{2}) significantly increased in leaves of plants exposed to cold stress and resulted in oxidative damage, indicated by an increase in malondialdehyde accumulation and membrane leakage. The …
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
An Improved Pvs2 Cryopreservation Technique For Ascocenda Wangsa Gold Orchid Using Protocorm-Like Bodies, Arulvilee Rajasegar, Asyraf Mansor, Ranjetta Poobathy, Elayabalan Sivalingam, Uma Rani Sinniah, Sreeramanan Subramaniam
Turkish Journal of Biology
Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate …
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Purification And Characterization Of A Cyanide-Degrading Nitrilase Fromtrichoderma Harzianum Vsl291, Jorge Ricaño-Rodriguez, Mario Ramirez Lepe
Turkish Journal of Biology
An intracellular nitrilase (Nit1) with cyanide-degrading activity was isolated from Trichoderma harzianum VSL291, cultivated on benzonitrile as the sole carbon source. Nit1 was purified to homogeneity by ion exchange and gel filtration chromatography with a recovery of 7.15% and a fold of 22.5. The molecular weight was estimated to be 47.7 kDa and the purified enzyme was sequenced with a system of liquid chromatography and mass spectrometry (LC-MS). The enzyme consists of 436 amino acids with a predicted molecular weight of 47.088 kDa. The sequence revealed conserved domains for a nitrilase super family such as putative active and binding sites …
Energy Efficiency Of The Sunlight Harvesting And Storing System In Bacterial Photosynthesis: Comparison With Semiconductor Photovoltaic Cells, Erkin Zakhidov, Mavluda Zakhidova, Abdumutallib Kokhkharov, Abdurasul Yarbekov, Vakhobjon Kuvondikov, Sherzod Nematov, Erkin Normatov, Aziz Saparbayev
Energy Efficiency Of The Sunlight Harvesting And Storing System In Bacterial Photosynthesis: Comparison With Semiconductor Photovoltaic Cells, Erkin Zakhidov, Mavluda Zakhidova, Abdumutallib Kokhkharov, Abdurasul Yarbekov, Vakhobjon Kuvondikov, Sherzod Nematov, Erkin Normatov, Aziz Saparbayev
Turkish Journal of Biology
The light phase of photosynthesis is considered a joint operation of 2 functional pigment-protein complexes: a light harvesting antenna, absorbing sunlight in a wide spectral range, and a reaction center, utilizing the energy of absorbed light quanta in photochemical charge separation reactions. These complexes allow converting solar energy to the energy of specific biomolecules with high quantum efficiency. However, before being transferred to reaction centers, the solar energy is stored in the lowest excited state of pigment molecules of the light harvesting antenna that partially convert light quantum energy into heat. These energy losses bring a significant reduction of energy …
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Investigation Of Mode Of Action Of Dna Insecticides On Thebasis Of Ldmnpv Iap-3 Gene, Volodymyr V. Oberemok, Palmah M. Nyadar
Turkish Journal of Biology
This study investigates the mechanism of action of DNA insecticides, a novel preparation against gypsy moth (Lymantria dispar) based on the DNA fragments of the antiapoptotic gene of its nuclear polyhedrosis virus. In our experiments gypsy moth showed significant susceptibility to applied DNA insecticides on the basis of BIR and RING domains fragments of LdMNPV IAP-3, whereas common fruit fly and tobacco hornworm did not. PCR with the same fragments of BIR and RING domains of the LdMNPV IAP-3 gene as primers revealed parts of the genomes of gypsy moth, common fruit fly, and tobacco hornworm. Part of the gypsy …
Td-Damd-Pcr Assays For Fingerprinting Of Commercial Carnations, Ayşe Gül İnce, Mehmet Karaca
Td-Damd-Pcr Assays For Fingerprinting Of Commercial Carnations, Ayşe Gül İnce, Mehmet Karaca
Turkish Journal of Biology
Dianthus L. is one of the highly valued plant species in the family Caryophyllaceae. More than 30,000 cultivars of commercial carnations have been recorded and there is a need for an effective and cheap method to reveal their genetic diversity and identify the cultivars. To the best of our knowledge this is the first report of implementation of the touchdown direct amplification of minisatellite-region DNA polymerase chain reaction (Td-DAMD-PCR) technique in the genus Dianthus. A total of 12 core minisatellite primers empirically selected from 22 primers were used in fingerprinting and phylogenetic studies of some commercial carnation cultivars. Analysis revealed …
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Effects Of Long-Term Exposure Of Female Rats To Low Levels Of Lead: Ovary And Uterus Histological Architecture Changes, Eugenia Dumitrescu, Viorica Chiurciu, Florin Muselin, Roxana Popescu, Diana Brezovan, Romeo Teodor Cristina
Turkish Journal of Biology
The aim of the current study was to evaluate lead accumulation in the ovaries, fallopian tubes, and uterus and to take note of any consequent histo-architectural changes. The experiment involved a 12-month chronic exposure of 28 Wistar female rats at sexual maturity (221 ± 0.88 g/individual) to lead acetate in drinking water. The rats were divided into 4 groups based on the level of lead exposure: E1 at 0.050 mg L^{-1}, E2 at 0.100 mg L^{-1}, E3 at 0.150 mg L^{-1} , and a control group that received tap water. Lead level evaluation was performed by atomic absorption spectrometry at …
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Enhancing The Expression Of Aspergillus Niger Ss-Mannanase In Pichia Pastoris By Coexpression Of Protein Disulfide Isomerase, Xiaoling Chen, Bo Zhou, Meng Xu, Zhiqing Huang, Gang Jia, Jiayun Qiao, Guangmang Liu
Turkish Journal of Biology
A gene encoding ß-mannanase from Aspergillus niger GIM3.452 was amplified and inserted into a pPIC9K vector. The resulting recombinant plasmid, pPIC9K-MAN, was transformed into Pichia pastoris GS115. One strain (GSKM-1) having the highest ß-mannanase activity of 26.6 U/mL was obtained. In order to increase the secretion of ß-mannanase in P. pastoris, we constructed a double recombinant yeast and made it coexpress protein disulfide isomerase. One strain (GSKZ\alphaM2) with the highest ß-mannanase activity of 40 U/mL was then obtained and used to optimize expression conditions. When the GSKZ\alphaM2 strain was induced under the optimized conditions (methanol concentration 1.5%, induction time 7 …
Artichoke Compound Cynarin Differentially Affects The Survival, Growth, And Stress Response Of Normal, Immortalized, And Cancerous Human Cells, Ceren Gezer, Sevi̇nç Yücecan, Suresh Inder Singh Rattan
Artichoke Compound Cynarin Differentially Affects The Survival, Growth, And Stress Response Of Normal, Immortalized, And Cancerous Human Cells, Ceren Gezer, Sevi̇nç Yücecan, Suresh Inder Singh Rattan
Turkish Journal of Biology
Cynarin (CYN) is the main derivative of caffeoylquinic acid, found in leaves and heads of artichoke. It may have hepatoprotective, antiatherosclerotic, antioxidative, choleretic, and cholesterol-lowering effects. We tested the effects of various doses of CYN on the proliferative potential, survival, morphology, and stress response (SR) markers heme oxygenase-1 (HO-1) and heat shock protein-70 (HSP70) in normal human skin fibroblasts (FSF-1), telomerase-immortalized mesenchymal stem cells (hTERT-MSC), and cervical cancer cells (HeLa). The effects of CYN on cell proliferation and morphology were dose- and cell type-dependent, with 500 µM CYN as the upper limit for all cell types. While the growth and …
Estradiol Receptors Mediate Estradiol-Induced Inhibition Of Mitochondrialca^{2+} Efflux In Rat Caudate Nucleus And Brain Stem, Snjezana Petrovic, Maja Milosevic, Danijela Ristic-Medic, Natasa Velickovic, Dunja Drakulic, Ivana Grkovic, Anica Horvat
Estradiol Receptors Mediate Estradiol-Induced Inhibition Of Mitochondrialca^{2+} Efflux In Rat Caudate Nucleus And Brain Stem, Snjezana Petrovic, Maja Milosevic, Danijela Ristic-Medic, Natasa Velickovic, Dunja Drakulic, Ivana Grkovic, Anica Horvat
Turkish Journal of Biology
Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca^{2+} efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl_2 (0.6?0.75 µCi ^45CaCl_{2}) was used for Ca^{2+} transport monitoring. The results revealed that in the presence of ER antagonist 7\alpha,17ß-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol (ICI 182,780) (1 µmol/L), the inhibitory effect of estradiol on mitochondrial Ca^{2+} efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of …
Two-Dimensional Gel-Based Serum Protein Profile Of Growing Piglets, Malgorzata Ozgo, Adam Lepczynski, Agnieszka Herosimczyk
Two-Dimensional Gel-Based Serum Protein Profile Of Growing Piglets, Malgorzata Ozgo, Adam Lepczynski, Agnieszka Herosimczyk
Turkish Journal of Biology
In the present study we have introduced a proteomic workflow, 2-dimensional electrophoresis coupled with matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) to create a 2-D map reflecting the characteristic pattern of serum proteins in growing piglets. Blood samples were collected from 6 piglets aged 50 days. Serum proteins were resolved at pH 3-10, followed by 12% SDS-PAGE. Reproducible protein spots (183) were picked from the gels and submitted for identification by peptide mass fingerprinting. As a result, 105 spots were positively identified, representing the products of 37 unique genes. Nearly half of the identified proteins (48.57%) were associated with …
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Prokaryotic Expression, Purification, Polyclonal Antibody Preparation, And Tissue Distribution Of Porcine Six1, Meng Xu, Xiaoling Chen, Zhiqing Huang, Wanxue Wen, Shuai Chang, Xiaoyan Wang, Daiwen Chen, Bing Yu, Junqiu Luo, Guangmang Liu
Turkish Journal of Biology
Sine oculis homeobox 1 (Six1), a member of the Six homeoproteins, plays an important role in skeletal myogenesis and the specification of myofiber diversity. In this study, in order to scale up the production of recombinant porcine Six1 (pSix1), a pET-30a(+)-pSix1 plasmid was constructed and transformed into Escherichia coli BL21 (DE3). The recombinant pSix1 could be induced for efficient expression with 2 mM IPTG for 2 h at 30 °C, yielding approximately 4.6 mg/L. The protein was then purified and identified by western blot, and it was used for preparing its polyclonal antibody. The recombinant pSix1 was tagged with a …
A Letter In Response To "Cancer Stem Cells: Emerging Actors In Both Basic And Clinical Cancer Research" Turk J Biol (2014) 38: ©Tübitak - Doi:10.3906/Biy-1406-93 Cancer Stem Cells (Cscs): Targets And Strategies For Intervention, Suresh Palamadai Krishnan
A Letter In Response To "Cancer Stem Cells: Emerging Actors In Both Basic And Clinical Cancer Research" Turk J Biol (2014) 38: ©Tübitak - Doi:10.3906/Biy-1406-93 Cancer Stem Cells (Cscs): Targets And Strategies For Intervention, Suresh Palamadai Krishnan
Turkish Journal of Biology
This letter to the editor highlights the important aspects of cancer stem cells and provides a deeper insight into the origin and characterization of these cells as well as the mechanisms contributing to drug resistance and relapse. The two currently available models (CSC and the clonal evolution model) need not be mutually exclusive to explain the origin of CSCs. The identification of CSCs (based on surface markers, drug efflux, enzyme activity, and signal transduction molecules and proteins involved in EMT processes) can help in discriminating these cells from other cell types. Moreover, CSCs prefer a hypoxic environment. This microenvironmental cue …
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Heterologous Expression And Purification Of Porcine Fat Mass Andobesity-Associated Gene In Escherichia Coli, Bo Zhou, Xiaoling Chen, Zhiqing Huang, Gang Jia, Guangmang Liu
Turkish Journal of Biology
A porcine fat mass and obesity-associated gene (pFTO) was cloned into the expression vector pET30a(+) and heterologously expressed in Escherichia coli. The effects of isopropyl ß-D-thiogalactopyranoside (IPTG) concentration and induction time on the expression of recombinant pFTO were assessed. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the molecular mass of the recombinant pFTO protein was about 56 kDa, and the recombinant protein could be induced efficiently in E. coli BL21 by the addition of 0.75 mM IPTG for 4 h at 30 °C. The recombinant pFTO fusion protein was purified using Ni-IDA affinity chromatography with a yield of about 1.8 …
In Vitro High Frequency Regeneration Through Apical Shoot Proliferation Of Hemianthus Callitrichoides `Cuba' - A Multipurpose Ornamental Aquatic Plant, Surendra Barpete, Sancar Fati̇h Özcan, Muhammad Aasim, Sebahatti̇n Özcan
In Vitro High Frequency Regeneration Through Apical Shoot Proliferation Of Hemianthus Callitrichoides `Cuba' - A Multipurpose Ornamental Aquatic Plant, Surendra Barpete, Sancar Fati̇h Özcan, Muhammad Aasim, Sebahatti̇n Özcan
Turkish Journal of Biology
Hemianthus callitrichoides 'Cuba' is an ornamental aquatic plant used in water gardens and phytoremediation. A protocol for its rapid and reproducible shoot organogenesis from apical shoot explants was developed. Optimum culture conditions for shoot proliferation were tested in MS media containing different concentrations of 6-($\gamma$,$\gamma$-dimethylallylamino)purine (2iP), 6-benzylaminopurine (BAP), 1-phenyl-3-1,2,3-thiadiazol-5-yl urea (TDZ), and gibberellic acid (GA3). Plant growth regulators significantly (P ≤ 0.001) affected the diameter, area, and fresh weight of shoot clumps. On the basis of plant growth, the maximum shoot diameter (3.06 cm), area (6.12 cm), and clump weight (2.85 g) of apical shoots were achieved on MS medium …
Analysis Of Structure And Differential Expression Of Pseudomonas Syringae 5-Like(Rps5-Like) Genes In Pathogen-Infected Vitis Flexuosa, Md. Zaherul Islam, Soon Young Ahn, Hae Keun Yun
Analysis Of Structure And Differential Expression Of Pseudomonas Syringae 5-Like(Rps5-Like) Genes In Pathogen-Infected Vitis Flexuosa, Md. Zaherul Islam, Soon Young Ahn, Hae Keun Yun
Turkish Journal of Biology
No abstract provided.
Mechanisms Of Pluripotency And Epigenetic Reprogramming In Primordial Germ Cells: Lessons For The Conversion Of Other Cell Types Into The Stem Cell Lineage, Suresh Palamadai Krishnan
Mechanisms Of Pluripotency And Epigenetic Reprogramming In Primordial Germ Cells: Lessons For The Conversion Of Other Cell Types Into The Stem Cell Lineage, Suresh Palamadai Krishnan
Turkish Journal of Biology
Primordial germ cells (PGCs) provide an excellent tool to better understand ancestor?descendent relationships as well as the efficiency and molecular mechanisms governing pluripotency in the reprogramming of somatic cells, since the latter type of cells have a relatively lower efficiency of conversion to pluripotent cells. This kind of comparison has gained credence from the commonalities regarding the expression of key transcription factors such as octamer-binding transcriptionhttp://en.wikipedia.org/wiki/Transcription_factor factor-4 (Oct3/4), SRY-related HMG box (Sox2), myelocytomatosis (c-Myc), and Nanog, as well as redundancy in terms of Kruppel-like factor 2 (Klf2), Kruppel-like factor 5 (Klf5), estrogen-related receptor beta (Esrrb), and estrogen-related receptor gamma (Esrrg) …
Organ-Specific Antioxidant Defenses And Ft-Ir Spectroscopy Of Muscles In Crucian Carp (Carassius Auratus Gibelio) Exposed To Environmental Pb2+, Sher Ali Khan, Xiaoyu Liu, Hong Li, Wenting Fan, Bakht Ramin Shah, Jingna Li, Lu Zhang, Suyan Chen, Sher Bahadar Khan
Organ-Specific Antioxidant Defenses And Ft-Ir Spectroscopy Of Muscles In Crucian Carp (Carassius Auratus Gibelio) Exposed To Environmental Pb2+, Sher Ali Khan, Xiaoyu Liu, Hong Li, Wenting Fan, Bakht Ramin Shah, Jingna Li, Lu Zhang, Suyan Chen, Sher Bahadar Khan
Turkish Journal of Biology
Being a nonessential element, lead (Pb2+) can be toxic in trace amounts for freshwater fish. The present study was designed to evaluate the induction of oxidative stress, tissue-specific antioxidant response, acetyl cholinesterase (AchE) activities, and biochemical content of muscle in Crucian carp (Carassius auratus gibelio) after 96 h of acute exposure and 21 days of chronic exposure to various environmental concentrations (5, 10, and 30 µg L-1) of waterborne Pb2+. The results indicated that exposure to 10 and 30 µg L-1 Pb2+ significantly altered the activities of catalase, superoxide dismutase, and glutathione peroxidase in different tissues, while malondialdehyde content increased …
Marker-Assisted Selection For Rice Brown Planthopper (Nilaparvata Lugens)Resistance Using Linked Ssr Markers, Mahmoodreza Shabanimofrad, Mohd Rafii Yusop, Sadegh Ashkani, Mohamed Hanafi Musa, Nur Azura Adam, Iffah Haifa, Abdul Rahim Harun, Mohammad Abdul Latif
Marker-Assisted Selection For Rice Brown Planthopper (Nilaparvata Lugens)Resistance Using Linked Ssr Markers, Mahmoodreza Shabanimofrad, Mohd Rafii Yusop, Sadegh Ashkani, Mohamed Hanafi Musa, Nur Azura Adam, Iffah Haifa, Abdul Rahim Harun, Mohammad Abdul Latif
Turkish Journal of Biology
No abstract provided.
Apoptotic Effect Of Cordycepin On A549 Human Lung Cancer Cell Line, Hardeep Singh Tuli, Gaurav Kumar, Sardul Singh Sandhu, Anil Kumar Sharma, Dharmbir Kashyap
Apoptotic Effect Of Cordycepin On A549 Human Lung Cancer Cell Line, Hardeep Singh Tuli, Gaurav Kumar, Sardul Singh Sandhu, Anil Kumar Sharma, Dharmbir Kashyap
Turkish Journal of Biology
Cordycepin, an active ingredient in the insect fungus Cordyceps militaris, is in a category of compounds that exhibit significant therapeutic activity. The aim of the present study was to investigate the effect of cordycepin on cell proliferation, apoptosis, and cell cycle in A549 human lung cancer cells. MTT assay was used to evaluate the cytotoxic effect of cordycepin on A549 cell growth. Apoptotic effect was observed using cell morphology, DAPI staining, and DNA fragmentation studies. Flow cytometry (FCM) analysis was used to analyze cell cycle status after cordycepin treatment. Furthermore, apoptosis was assayed using annexin V-Alexa Fluor 488. Results of …
Molecular Characterization Of Diverse Wheat Germplasm For Puroindolineproteins And Their Antimicrobial Activity, Vishal Chugh, Kirandeep Kaur, Dharmendra Singh, Vinod Kumar, Harinderjit Kaur, Harcharan Singh Dhaliwal
Molecular Characterization Of Diverse Wheat Germplasm For Puroindolineproteins And Their Antimicrobial Activity, Vishal Chugh, Kirandeep Kaur, Dharmendra Singh, Vinod Kumar, Harinderjit Kaur, Harcharan Singh Dhaliwal
Turkish Journal of Biology
Thirty different wheat genotypes, including landraces, cultivars, and related wild species, were analyzed for puroindoline genes and proteins. Full-length Pina and Pinb genes were amplified using gene-specific markers where 13 genotypes amplified both genes, while 17 genotypes had either one or none of the puroindoline genes. None of the puroindoline genes were detected in any of the Triticum durum cultivars. Genotypes with both puroindoline genes had a soft grain texture, while other genotypes had hard grains. Related nonprogenitor species with A, C, M, U, and S genomes also showed the presence of either one or both the puroindoline genes, indicating …
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Histone Deacetylase Inhibitory Activity Of Hydroxycapsaicin, A Synthetic Derivative Of Capsaicin, And Its Cytotoxic Effects Against Human Colon Cancer Cell Lines, Thanaset Senawong, Paweena Wongphakham, Thussanee Saiwichai, Chanokbhorn Phaosiri, Pakit Kumboonma
Turkish Journal of Biology
Capsaicin possesses cytotoxic/anticancer activity and shares some common structural features, including a benzene ring and a long hydrophobic carbon tail, with the histone deacetylase (HDAC) inhibitors trichostatin A and suberoylanilide hydroxamic acid. The aims of this study were to investigate HDAC inhibitory and cytotoxic activities of a synthetic derivative of capsaicin, hydroxycapsaicin (6-hydroxy-N-(4-hydroxy-3-methoxybenzyl)-8-methylnonenamide), in colon cancer cell lines. Hydroxycapsaicin inhibited HDAC activity in vitro (IC50 = 72 µM) much more effectively than the prototype capsaicin (IC50 > 13.1 mM) and also exhibited HDAC inhibitory activity in human cells (HeLa cells). MTT assay demonstrated that hydroxycapsaicin was less toxic than capsaicin against …
Eugenol Biologic Activity In Immunosuppressed Rat Females With Candida Albicans Genital Infection: Histocytological Changes, Romeo Teodor Cristina, Diana Obistioiu, Eugenia Dumitrescu, Ileana Nichita, Florin Muselin, Diana Brezovan, Mihai Sorin Cernea
Eugenol Biologic Activity In Immunosuppressed Rat Females With Candida Albicans Genital Infection: Histocytological Changes, Romeo Teodor Cristina, Diana Obistioiu, Eugenia Dumitrescu, Ileana Nichita, Florin Muselin, Diana Brezovan, Mihai Sorin Cernea
Turkish Journal of Biology
From our prior studies concerning eugenol's biologic activity we observed the very good in vitro antifungal efficiency of this natural compound. Those positive results generated the need to supplement the available information with a comparative in vivo animal model. In this context, our current study was proposed to ascertain and compare the effects of eugenol with nystatin with a placebo control group (saline solution) by evaluating the cytohistological alterations in immunosuppressed Wistar female rats genitally infected with Candida albicans. The observed histological alterations were most apparent in the placebo group. Candidiasis affected the liver, ovaries, kidneys, and spleen of the …
Investigation Of The In Vivo Interaction Between Ss-Lactamase And Its Inhibitor Protein, Ni̇lay Büdeyri̇ Gökgöz, Si̇may Yalaz, Naze Gül Avci, Gi̇zem Buldum, Eli̇f Özkirimli Ölmez, Berna Sariyar Akbulut
Investigation Of The In Vivo Interaction Between Ss-Lactamase And Its Inhibitor Protein, Ni̇lay Büdeyri̇ Gökgöz, Si̇may Yalaz, Naze Gül Avci, Gi̇zem Buldum, Eli̇f Özkirimli Ölmez, Berna Sariyar Akbulut
Turkish Journal of Biology
The affinity of ß-lactamase inhibitory protein (BLIP) for TEM-1 ß-lactamase has raised hopes in the challenge of protein-based inhibitor discovery for ß-lactamase-mediated antibiotic resistance. Currently, the effect of the formation of the ß-lactamase:BLIP complex in vivo in ß-lactam resistant bacteria is an open question. The scarcity of information to the extent to which BLIP can impair ß-lactamase activity inside cells has urged us to assess the in vivo efficacy of BLIP as a potent ß-lactamase inhibitor. To this end, ß-lactamase and BLIP were coexpressed in Escherichia coli. Simultaneous expression of ß-lactamase and BLIP and the formation of the TEM-1 ß-lactamase:BLIP …