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Articles 1531 - 1560 of 1681
Full-Text Articles in Biology
The Effect Of Cadmium Nitrate On The Longevity Of Drosophilamelanogaster, Eli̇f Yeşi̇lada, Lütfi̇ye Gelegen
The Effect Of Cadmium Nitrate On The Longevity Of Drosophilamelanogaster, Eli̇f Yeşi̇lada, Lütfi̇ye Gelegen
Turkish Journal of Biology
The effect of cadmium nitrate on the longevity of Drosophila melanogaster was tested. When cadmium nitrate was given throughout the adult life it caused a decrease in the mean life-span of flies in all treated groups. Furthermore, the effect of cadmium nitrate on young populations was also compared with aged populations. The young populations survived longer than aged populations with the food medium containing cadmium nitrate.
The Isolation And Characterization Of Glucoamylase Enzyme Of Anaspergillus Niger Natural Isolate, Gökhan Coral, Ömer Çolak
The Isolation And Characterization Of Glucoamylase Enzyme Of Anaspergillus Niger Natural Isolate, Gökhan Coral, Ömer Çolak
Turkish Journal of Biology
An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.
Obtaining Biosurfactant From Olive Oil Mill Effluent (Oome) Usingpseudomonas Sp., Uğur Sidal, Nazi̇f Kolankaya, Cengi̇z Kurtonur
Obtaining Biosurfactant From Olive Oil Mill Effluent (Oome) Usingpseudomonas Sp., Uğur Sidal, Nazi̇f Kolankaya, Cengi̇z Kurtonur
Turkish Journal of Biology
Olive oil mill effluent (OOME) is one of the major pollutants produced by the agricultural industry in Mediterranean countries. The olive oil mills in these countries generate about 2-3 million tonnes of effluent annually. OOME is a black liquor containing a water-soluble fraction of ripe olives and water that is used in the process of olive oil extraction. This waste has a high mineral content and a chemical oxygen demand of 25.000-100.000 mg/l. depending on the olive oil extraction process used. This study presents a process for biosurfactant as rhamnolipid production by Pseudomonas sp. from olive oil mill effluent (OMME). …
The Seasonal Variation In Total Lipid And Fatty Acid Contents Of Thegonads Of Cyprinion Macrostomus (Heckel, 1843), Kubi̇lay Meti̇n, Mehmet Ali̇ Akpinar
The Seasonal Variation In Total Lipid And Fatty Acid Contents Of Thegonads Of Cyprinion Macrostomus (Heckel, 1843), Kubi̇lay Meti̇n, Mehmet Ali̇ Akpinar
Turkish Journal of Biology
The seasonal variation in total lipid and total fatty acid contents of the gonads of Cyprinion macrostomus(HECKEL, 1843) living in the Topardiç Brook (Kangal-Sivas) were investigated. It was observed that the variations in the gonad weights and the amount of total lipid and total fatty acid in the gonad of the male and female fish were more noticeable especially at the time of gonad development and the spawning period. The total lipid and total fatty acid amount which increased during the spawning period showed a decrease at the end of this period.
Study Of Adventitious Shoot Formation In Leaves Of Grape (Vitis Spp.), Ni̇lgün Göktürk Baydar
Study Of Adventitious Shoot Formation In Leaves Of Grape (Vitis Spp.), Ni̇lgün Göktürk Baydar
Turkish Journal of Biology
The effects of genotype, source of explant and nutrient medium on adventitious shoot formation were examined in the leaves of 5 grape cultivars (Atasarisi, Çavus, Kalecik Karasi, Sultani çekirdeksiz and Yapincak), 2 rootstocks (Kober 5BB ( V. berlandieri x V. riparia) and 41 B M.G. (Chasselas x V. berlandieri)) by organogenesis. In this study, petioles and laminae isolated from in vitro shoots were used as the sources of explant. The highest rates of adventitious shoot formation were obtained from petiole explants. Leaf explants were cultured on MS- and NN- based regeneration media containing different concentrations of BAP and NAA. It …
Karyotype Analysis In Chalcalburnus Mossulensis (Heckel, 1843), Süleyman Gül, Ahmet Çolak, İlhan Sezgi̇n
Karyotype Analysis In Chalcalburnus Mossulensis (Heckel, 1843), Süleyman Gül, Ahmet Çolak, İlhan Sezgi̇n
Turkish Journal of Biology
Karyotype analysis was performed in Chalcalburnus mossulensis (Heckel, 1843) belonging to the carp family by investigating the number and structures of their chromosomes. The fish used in this study were caught with fishing nets from Kizilirmak river and taken to the laboratory. They received an abdominal injection of 0.6 mg colchicine (Colchicum Dispert) dissolved in 1 ml of distilled water per 1 g body weight. As a result of the C-metaphase investigation, it was determined that C. mossulensis had 2n=48 chromosomes. Their karyotypes were determined to be composed of 6 metacentric, 10 submetacentric, and 8 acrocentric chromosome pairs. We were …
Boron Accumulation In Carp Tissues ( Cyprinus Carpio L., 1758) In Damlakes Çötören And Kunduzlar (Kırka-Eskişehir), Şaki̇r Özkurt
Boron Accumulation In Carp Tissues ( Cyprinus Carpio L., 1758) In Damlakes Çötören And Kunduzlar (Kırka-Eskişehir), Şaki̇r Özkurt
Turkish Journal of Biology
Samples of water, bottom clay, plankton and fish were collected over a period of one year. The boron contents of the samples taken were determined in the laboratory. The differences and relationships in the obtained data were identified using various statistical methods. Boron gradually accumulates in the plankton, bottom clay and water of Çatören and Kunduzar. Boron collects in the tissues of the fish Cyprinus carpio in both lakes, in the musce, brain and liver in Çatören, and in the musce, liver and brain in Kunduzlar. Boron pollution is more serious in Çatören dam than in Kunduzlar. It was observed …
The Effects Of Heavy Metals On The Pollen Germination And Pollen Tubegrowth Of Apples (Malus Sylvestris Miller Cv. Golden), Ömer Munzuroğlu, Nazmi̇ Gür
The Effects Of Heavy Metals On The Pollen Germination And Pollen Tubegrowth Of Apples (Malus Sylvestris Miller Cv. Golden), Ömer Munzuroğlu, Nazmi̇ Gür
Turkish Journal of Biology
The effect of heavy metals, namely mercury (Hg^{+2}), zinc (Zn^{+2}), nickel (Ni^{+2}) and cobalt (Co^{+2}), which were applied in the form of chloride salt, and that of lead (Pb^{+2}) and cadmium (Cd^{+2}) applied in the form of nitrate salt, on the pollen germination and tube growth were investigated on apples ( Malus sylvestris cv. Golden). According to the results obtained, these heavy metals led to a significant decrease in the pollen germination and tube growth. It was found that there was a parallelism between the concentration of heavy metal salt, and pollen germination and tube growth. Among the heavy metals …
Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard
Prevention Of Hydrogen Peroxide And Cisplatininduced Apoptosis By Intracellular Catalaseoverexpression, H. Barbaros Oral, Andrew J.T. George, Dorian O. Haskard
Turkish Journal of Biology
Apoptosis induced by hydrogen peroxide (H_2O_2 ) was investigated in Chinese hamster ovarian (CHO) cells, and compared to apoptosis induced by cisplatin (25 µM), a well-established apoptosis-inducing agent. In preliminary experiments, CHO cells were treated with low concentrations of H_2O_2 (250-500 µM) continuously or for 3 hours, followed by washing and further incubation in complete growth medium for 1-10 days. Fluorescence microscopy of acridine orange-stained cells indicated morphological changes of apoptosis. Similar changes were observed in cisplatin-treated cells. DNA-laddering also revealed that DNA fragmentation occurred in both adherent and non-adherent cells by day 2. To prevent H_2O_2 -mediated apoptosis, the …
Production Of Monoclonal Antibodies Specific Forprogesterone, Estradiole By Simultaneous Injectionof Different Steroids, Fatma Şahi̇ngöz Yücel, Beyazit Çirakoğlu
Production Of Monoclonal Antibodies Specific Forprogesterone, Estradiole By Simultaneous Injectionof Different Steroids, Fatma Şahi̇ngöz Yücel, Beyazit Çirakoğlu
Turkish Journal of Biology
We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone (MAM 3C2, MAM 3E3) and one of them reacted specifically with estradiol (MAM 7H7). Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found …
Effects Of Ph On The Genesis Of Membrane Potentialchanges At Fertilization In The Egg Of The Frog Ranacameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Effects Of Ph On The Genesis Of Membrane Potentialchanges At Fertilization In The Egg Of The Frog Ranacameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Turkish Journal of Biology
The apparent change in the egg membrane potential at fertilization gives rise to fertilization potential (FP) . FP is the initial, transient electrical block to polyspermy as shown in most species especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until the permanent, mechanical block is set up. Polyspermy is lethal in most species, and for a successful fertilization resting membrane potential (RMP) and FP of the egg have to be held in optimum ranges. Shifts in environmental pH may interfere with a successful fertilization by affecting these bioelectrical potentials. In the present study, …
Characterization Of Endoxylanase Activity Fromthermomonospora Fusca Bd25, Müni̇r Tuncer
Characterization Of Endoxylanase Activity Fromthermomonospora Fusca Bd25, Müni̇r Tuncer
Turkish Journal of Biology
Thermomonospora fuscaBD25 produces relatively high levels of activity of endoxylanases. Only oat spelt xylan, RBB-xylan and birchwood xylan reacted with endoxylanases with the maximum specific activity of 47.65 U mg -1 protein on birchwood xylan. The endoxylanase remained stable up to 70 °C. At 60 °C endoxylanase activity showed stability (92 %) for 16 h, however it showed a half-life of 8 h at 70 °C and 30 min at 80 °C in the absence of substrate at pH 8.0. The relative endoxylanase activity in the pH ranges of 6.5 to 9.5 remained between 68 % and 72 % of …
Characterization Of \Beta-Xylosidase And\Alpha-L-Arabinofuranosidase Activities Fromthermomonospora Fusca Bd25, Müni̇r Tuncer
Characterization Of \Beta-Xylosidase And\Alpha-L-Arabinofuranosidase Activities Fromthermomonospora Fusca Bd25, Müni̇r Tuncer
Turkish Journal of Biology
Thermomonospora fuscaBD25 produces relatively high levels of activity of \beta-xylosidase and \alpha-L-arabinofuranosidase. The aim of the work described in this study was to characterize some properties of \beta-xylosidase and \alpha-L-arabinofuranosidase produced by T. fusca BD25 when growing on oat spelt xylan as the main carbon and energy sources. The substrates p-NPX and p-NPA reacted with \beta-xylosidase and \alpha-L-arabinofuranosidase with specific activities of 4.01 U mg -1 protein and 0.35 U mg -1 protein, respectively. The \beta-xylosidase remained stable at up to 65 °C, but \alpha-L-arabinofuranosidase lost 10 % of its maximum activity at 55 °C. \beta-xylosidase and \alpha-L-arabinofuranosidase activities remained …
Production Of Auxin And Abscisic Acid Byphanerochaete Chrysosporiumme446 Immobilizedon Polyurethane Foam, Serpi̇l Ünyayar, Eli̇f Ünal, Ali̇ Ünyayar
Production Of Auxin And Abscisic Acid Byphanerochaete Chrysosporiumme446 Immobilizedon Polyurethane Foam, Serpi̇l Ünyayar, Eli̇f Ünal, Ali̇ Ünyayar
Turkish Journal of Biology
The fungus Phanerochaete chrysosporiumME446 was entrapped on polyurethane foam to synthesize auxin and abscisic acid (ABA). The maximum amounts of auxin produced on the 18 th day in both free and immobilized cells were 55.91mg/mL and 76.07mg/mL, respectively. Maximum ABA concentrations on the 12 th day in both free and immobilized cells were 6.87 mg/mL and 10.69 mg/mL, respectively. An immobilized system of auxin and ABA production resulted in a comparatively higher yield than free fungal mycelium.
Temporal And Spatial Determination Of Germinbiosynthesis In Wheat Tissues, Mahmut Çalişkan, Andrew C. Cuming
Temporal And Spatial Determination Of Germinbiosynthesis In Wheat Tissues, Mahmut Çalişkan, Andrew C. Cuming
Turkish Journal of Biology
In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.
Cultural Characteristics Of Morchella Esculentamycelium On Some Nutrients, Peri̇han Güler, Oğuz Arkan
Cultural Characteristics Of Morchella Esculentamycelium On Some Nutrients, Peri̇han Güler, Oğuz Arkan
Turkish Journal of Biology
The cultural characteristics of Morchella esculenta mycelium were investigated in potato dextrose agar, malt extract agar and complete medium yeast plates prepared with casein (cas), Casamino Acids (caa), peptone (p) and sodium nitrate (NaNO 3 ). Aerial and vegetative mycelium types were determined in the agar media with cas, caa and p. The third colonial characteristic was observed in the agar media with NaNO 3 . In this media, a colonization increase in circular forms spreading from the centre to the edges was determined. Mycelium colonization periods were 20-24 days in agar media with NaNO 3 .
Bacteriocin Typing Of Some Turkish Isolates Ofpseudomonas Syringae Pv. Phaseolicola, Kiymet Güven
Bacteriocin Typing Of Some Turkish Isolates Ofpseudomonas Syringae Pv. Phaseolicola, Kiymet Güven
Turkish Journal of Biology
Eighty-six Pseudomonas syringae pv. phaseolicola isolates collected from different bean growing areas in Eskişehir were typed for the production of bacteriocin.All the isolates tested produced bacteriocin and 24 bacteriosin groups were determined. No correlation was found between the bacteriocin groups and geographical origin. Authentic isolates of the bacterium representing 3 different races were also tested for bacteriocin production and bacteriocin types did not correlate with the races.
Electrophoretic Analysis Of Candida Albicans Isolatescollected From Turkish Hospitals By Native-Polyacrylamidegel Electrophoresis (N-Page), Özlem Osmanağaoğlu, Sefa Can Saçilik, Ufuk Gündüz, Nurten Altinlar, Cumhur Çökmüş
Electrophoretic Analysis Of Candida Albicans Isolatescollected From Turkish Hospitals By Native-Polyacrylamidegel Electrophoresis (N-Page), Özlem Osmanağaoğlu, Sefa Can Saçilik, Ufuk Gündüz, Nurten Altinlar, Cumhur Çökmüş
Turkish Journal of Biology
Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicansstrains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicansisolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. …
Protein Profiles And Prevalence Of Methicillinresistant Staphylococcus Aureus (Mrsa) In Gülhanemilitary Academy Hospital In Turkey, Sefa Can Saçilik, Özlem Osmanağaoğlu, Özgül Kisa, Ahmet Başustaoğlu, Cumhur Çökmüş
Protein Profiles And Prevalence Of Methicillinresistant Staphylococcus Aureus (Mrsa) In Gülhanemilitary Academy Hospital In Turkey, Sefa Can Saçilik, Özlem Osmanağaoğlu, Özgül Kisa, Ahmet Başustaoğlu, Cumhur Çökmüş
Turkish Journal of Biology
The present study was undertaken to determine the relationship between Methicillin-Resistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. In 1997, at this hospital, the percentage of S. aureus among all nosocomial isolates was 20% and the percentage of MRSA among these S. aureus isolates was 51%. Both sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-Page (N-PAGE) were used for typing MRSA isolates which have common antibiotic susceptibility test results. MRSA isolates were also evaluated according to the whole-cell proteins (WCPs) and filtrate proteins (FPs). Using these methods, we concluded that …
Availibility Of Use Of Total Extracellular Proteins Insds-Page For Characterization Of Gram-Positive Cocci, Sefa Can Saçilik, Özlem Osmanağaoğlu, Ufuk Gündüz, Cumhur Çökmüş
Availibility Of Use Of Total Extracellular Proteins Insds-Page For Characterization Of Gram-Positive Cocci, Sefa Can Saçilik, Özlem Osmanağaoğlu, Ufuk Gündüz, Cumhur Çökmüş
Turkish Journal of Biology
There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDS-PAGE of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and characteristic banding patterns for the study of the characterization of gram-positive cocci. This method was found …
Nonspecific Esterase Patterns Of Rattus Norvegicus(Berkenhout, 1769) In Western Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Nonspecific Esterase Patterns Of Rattus Norvegicus(Berkenhout, 1769) In Western Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
Nonspecific esterases in the muscle, heart, kidney and liver tissues of wild rats (Rattus norvegicus) were examined by horizontal starch gel electrophoresis. Zymogram bands corresponding to Es-10 and Es-4 in the muscle, heart, kidney and liver homogenates, Es-3 and Es-17 in the kidney and liver, Es-18 in the liver tissue, Es-15 in kidney and liver, Es-2 in the muscle and heart, Es-16 in the heart tissue were detected. These findings showed that nonspecific esterases are very polymorphic and tissue-specific, and are not of taxonomic importance for the populations of Rattus norvegicus.
Antiviral Activity Of Some Plant Extracts On Thereplication Of Autographa Californicanuclearpolyhedrosis Virus, Ömer Ertürk, Zi̇hni̇ Demi̇rbağ, Ali̇ Osman Beldüz
Antiviral Activity Of Some Plant Extracts On Thereplication Of Autographa Californicanuclearpolyhedrosis Virus, Ömer Ertürk, Zi̇hni̇ Demi̇rbağ, Ali̇ Osman Beldüz
Turkish Journal of Biology
The effect of crude extracts, obtained from Aconitum nasutum, Daphne glomerata, Hypericum androsaemum, Laurus nobilis, Nerium oleander, Olea europaea, Prunus laurocerasus, Punica granatum, Rhododendron caucasicum, and Urtica dioica on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) grown in Spodoptera frugiperda cell culture were investigated by observing changes in cytophatic effects, progeny virus concentration and viral protein (polyhedrin) synthesis. We determined an inhibitory effect on cytophatic changes, virus concentration and synthesis of polyhedrin by utilizing the extract of A conitum nasutum, Hypericum androsaemum, Laurus nobilis, Rhododendron caucasicum, and Urtica dioica plants. On the other hand, the effects of Daphne …
The Possibility Of Using Crustacean Waste Products(Cwp) On Rainbow Trout ( Oncorhynchus Mykiss)Feeding, Yeşi̇m Özoğul
The Possibility Of Using Crustacean Waste Products(Cwp) On Rainbow Trout ( Oncorhynchus Mykiss)Feeding, Yeşi̇m Özoğul
Turkish Journal of Biology
Crustacean waste product (CWP) generated from the fishing industry could be used in diets for rainbow trout as an alternative animal protein. Some experiments have been undertaken with penaid species, showing that it is possible to partially replace fish meal with CWP in penaid feeds. This study has been designed to assess whether CWP could be used to partially replace fish meal in diets for rainbow trout. The results show that CWP improved the digestibility of the diet, yielding the highest performance, and also that the addition of large amounts of CWP to a low-protein diet did not depress appetite. …
Blood Serum Proteins Of The Genus Mesocricetusnehring, 1898 (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Blood Serum Proteins Of The Genus Mesocricetusnehring, 1898 (Mammalia: Rodentia) In Turkey, Reyhan Çolak, Nuri̇ Yi̇ği̇t, Ercüment Çolak, Mustafa Sözen, Şaki̇r Özkurt
Turkish Journal of Biology
In this study, 18 live specimens collected from six localities in the distribution areas of Mesocricetus brandti and Mesocricetus auratus were examined using SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis). The findings showed that there is no difference in globulin and albumin proteins of M. brandti and M. auratus, and supported the claim that M. auratus is very closely related to M. brandti.
Humerus Primer Ossification Zone Development In Rat Fetuses: A Histological And Histochemical Study, İsmai̇l Üstünel, Gökhan Akkoyunlu, Erdoğan Kocamaz, Ramazan Demi̇r
Humerus Primer Ossification Zone Development In Rat Fetuses: A Histological And Histochemical Study, İsmai̇l Üstünel, Gökhan Akkoyunlu, Erdoğan Kocamaz, Ramazan Demi̇r
Turkish Journal of Biology
In this investigation of the primary ossification zones of 14-18 day-old fetal rat humeri by histological, histochemical and histoenzymatical methods, 30 rat fetuses of the species Rattus norvegicus were used. The humeral diaphysis samples of the fetuses were subjected to alcian blue-heamatoxylin, periodic acid Schiff (PAS)-heamatoxylin, alcian blue-PAS-heamatoxylin, alizarin red and toluidine blue, alkaline phosphatase (ALP), adenosine triphosphatase (ATPase) and acid phophatase (ACP) staining techniques. The results can be summarized as follows: On day 14, no ossification signs were evident yet, whereas on day 15, the initiation of degenerations in some chondrocyte cytoplasms and the formation of osteoblastic cells on …
Production Of Uricase Enzyme From Aspergillus Niger And Determination Of Some Factors Affecting The Activity, Fi̇gen Ertan, Erol Aksöz
Production Of Uricase Enzyme From Aspergillus Niger And Determination Of Some Factors Affecting The Activity, Fi̇gen Ertan, Erol Aksöz
Turkish Journal of Biology
This study was undertaken to obtain uricase enzyme from Aspergillus niger and also to determine the production conditions and the effects of some factors on enzyme activity. The results of the experiments, in consideration of the production of the uricase enzyme and the factors affecting the production, demonstrated that enzyme activity was maximum when the production time was 3 days at a temperature of 30ºC with the initial pH at 6.0 and inductor concentration at 0.1%. It was determined that ammonium compounds, which were added to the production medium, inhibited enzyme synthesis, and that MgS0_4 has an important role in …
Isolation, Identification And Toxigenic Tests Of Aeromonas Hydrophila From Several Sources In Izmir, Ataç Uzel, Füsun Uçar
Isolation, Identification And Toxigenic Tests Of Aeromonas Hydrophila From Several Sources In Izmir, Ataç Uzel, Füsun Uçar
Turkish Journal of Biology
A total of 43 samples (4 sea water, 5 water, 4 river water, 10 oyster and 10 fish samples) from Izmir were examined for the presence of Aeromonas hydrophila. Alkaline peptone Water (APW) for the environmental samples and Trypticase Soy Broth with Ampicillin and Toluidine Blue (TSBA) for the food samples were used for enrichment of the samples. In isolation, 5 different selective media were used: DNase Agar with Toluidine blue and Ampicillin (DNTA), Aeromonas Agar (AA), Rimler Shotts Agar (RSA), mA Agar and Aeromonas Mediums Base (AMB). Identification of the isolates was performed according to Bergey's Manual of Determinative …
Determination Of Organophosphate Resistance-Dependent Over-Produce Esterase Allel Type With Electrophoresis In The Malaria Vector Species Anopheles Sacharovi And Culex Pipiens (Diptera: Culicidae), Ümi̇t Lüleyap, Davut Alpteki̇n, Hali̇l Kasap, Mülki̇ye Kasap
Determination Of Organophosphate Resistance-Dependent Over-Produce Esterase Allel Type With Electrophoresis In The Malaria Vector Species Anopheles Sacharovi And Culex Pipiens (Diptera: Culicidae), Ümi̇t Lüleyap, Davut Alpteki̇n, Hali̇l Kasap, Mülki̇ye Kasap
Turkish Journal of Biology
In this study, over-produce esterase allele types dependent on organophosphate resistance in mosquitoes were determined by cellulose acetate gel electrophoresis in the human malaria vector species Anopheles sacharovi and Culex pipiens in Çukurova (Adana). We found A_2 and A_5 allele bands with some unknown and unidentified gel bands in some An. sacharovi field samples, of which some bands seemed to match with over-produce esterase. We identified A_2-B_2 allele types for all the Cx. pipiens samples collected in this study.
Histological Changes Induced By Sialoadenectomy And Flutamide On The Epididymal Tissue And Epididymal Abnormalities, M.Aydin Ketani̇, Ahmet Ersay, Yusuf Nergi̇z, Mustafa Deni̇z, Şennur Ketani̇
Histological Changes Induced By Sialoadenectomy And Flutamide On The Epididymal Tissue And Epididymal Abnormalities, M.Aydin Ketani̇, Ahmet Ersay, Yusuf Nergi̇z, Mustafa Deni̇z, Şennur Ketani̇
Turkish Journal of Biology
In this study the purpose was to determine the effect of sialoadenectomy (Maternal Epidermal Growth Factor deficiency) on epididymal development and to compare it with that of antiandrogen (Flutamide) application.Forty 12-week-old, sexually mature and pregnant Sprague-Dawley rats were chosen as the animal model. They were divided into four groups; Group I : Control group, Group II : Sialoadenectomized (mated on 28^{th} day), Group III: Flutamide applied (between gestational day 11 and 21), Group IV: Sialoadenectomy + Flutamide applied. After birth, all male rats were observed morphologically on the 60th day and sacrificed on the 60^{th} day postpartum under general anaesthesia. …
Effects Of Endosulfan On The Isolated Sciatic Nerve Of The Frog, Ülkü Çömelekoğlu, Belgi̇n Büyükakilli, Aynur Avci Özge
Effects Of Endosulfan On The Isolated Sciatic Nerve Of The Frog, Ülkü Çömelekoğlu, Belgi̇n Büyükakilli, Aynur Avci Özge
Turkish Journal of Biology
The purpose of this study was to investigate the effect of endosulfan on the isolated sciatic nerve of the frog. The nerve was exposed to 0.5 ppm endosulfan for 60 min and then compound action potentials were recorded extracellulary. The latencies, amplitudes, durations of depolarization and repolarization and threshold voltage were measured (n=30). Conduction velocities were calculated using the nerve lengths and latency values of each nerve. It was observed that values of amplitude and duration of repolarization were significantly higher (p