Biology Commons^™

Population Genetics Of Cownose Rays, Rhinoptera Spp. In The Western Atlantic, Helen Weber

Master's Theses

Using molecular tools (e.g., the NADH subunit II mitochondrial gene), this study was the first to document the Brazilian cownose ray, Rhinoptera brasiliensis, within the northwestern Atlantic (NWA), and was the first study to examine population structure in R. brasiliensis within the NWA or northern Gulf of Mexico (GMX), revealing novel insights into the population biology of the animal and extending its range by nearly 1,500 km. This study also examined the sympatrically occurring American cownose ray, R. bonasus, and found population structure between the NWA and the GMX and the NWA and the southwestern Atlantic (SWA). High …

Seed Longevity And Climatic Tolerance Of San Joaquin Wooly-Threads (Monolopia Congdonii; Asteraceae) An Endangered Plant From The San Joaquin Desert, California, Paul Excoffier

Master's Theses

San Joaquin wooly-threads (Monolopia congdonii; Asteraceae) is a federally-listed, endangered annual plant species from the desert areas of the San Joaquin Valley. Its limited range puts it at risk of extinction if the climate changes in such a way as to hinder its growth and reproduction. The primary aims of the study were to 1) determine how long-lived the seeds of the M. congdonii are, a key determinant of survival of desert annual plant populations through long droughts and 2) determine how severely hotter, drier conditions impact the ability of emerged plants to grow and reproduce. Secondarily, I …

Understanding Rare Species In California: An Assessment Of Camatta Canyon Amole (Hooveria Purpurea Var. Reducta) And A Meta-Analysis Of California Rare Plants In Literature, Kieran N. Althaus

Master's Theses

California is currently in the midst of a biodiversity crisis. There are approximately 5,000 native species of plants in California, a quarter of which are considered rare. Determining threats to these rare plants is often times difficult. Despite California's botanical resources, we still know very little about much of California’s rare plants. San Luis Obispo County is home to 2,000 of California’s native plant taxa, one- third of which are rare or endemic to the county. These species are of great local and environmental concern.

In Chapter 1, we attempted to assess the impact of non native species on a …

A Comparison Of Natural, Living, And Hardened Shorelines Ability To Prevent Coastal Erosion And Maintain A Healthy Ecosystem, Gabrielle Spellmann

Master's Theses

It is important to find a suitable method to protect the U.S. Gulf Coast shoreline, since its’ low elevation and the Loop current make it vulnerable to sea level rise. I focused on two manmade methods, hardened, and living shorelines, of coastal protection for when the natural marsh suffers excess erosion rates. Living shorelines are a suite of shoreline conservation and restoration techniques that usually involve some sort of hardened structure that dampens wave energy so that the native vegetation behind it can take root and stabilize the shoreline. This study looked at six different sites, all containing a natural, …

Elucidating The Function Of An Evolutionarily Conserved Col2Α1 Isoform During Vertebrate Development, Charles Lantz H. Ingersoll

Master's Theses

ABSTRACT

Formation of skeletal structural elements during vertebrate embryogenesis is largely a

conserved evolutionary process that employs numerous conserved genes and pathways. One

critical gene in this process is the highly conserved vertebrate Type II Collagen alpha 1 (col2α1).

It produces multiple splice isoforms that have different functions and expression patterns during

skeletogenesis. Two of the primary isoforms are of great interest; variant 1, the primary

embryonic isoform, and variant 2, a post embryonic isoform. While the more commonly known

post-embryonic isoform has been well studied over the last 30 years, identification of a unique

role for the evolutionarily conserved …

Development And Characterization Of A Tissue-Specific Reporter Line For Monitoring Circadian Clock Transcriptional Activity, Lilyan Mather

Master's Theses

Daily rhythms in physiology and behavior are produced by a circadian system consisting of a central clock located in the brain and peripheral clocks located in various peripheral tissues. Circadian clocks track time of day through the presence of a molecular clock that functions as a transcriptional-translational feedback loop. In the nucleus, the transcription factors CLOCK (CLK) and CYCLE (CYC) activate transcription of clock genes period (per) and timeless (tim). PER and TIM proteins slowly accumulate in the cytoplasm before entering the nucleus, where they inhibit CLK and CYC, and therefore repress their own transcription. Degradation of nuclear PER and …

Comparison Of Feeding Functional Morphology Development In North American Esocids, Cheryl Theile

Master's Theses

Esocidae is a family of freshwater fishes within order Esociformes known for its long, cylindrical bodies, duck-bill snouts, and an impressive number of sharp teeth. There are four species, including two subspecies, in North America with areas of overlap of their geographic distribution. These ambush predators change diets from invertebrate prey to vertebrate prey during their first year of life, and the timing of this shift varies between species. In particular, the sister species Esox lucius (Northern Pike) and Esox masquinongy (Muskellunge) overlap in the upper Midwest, but E. masquinongy transitions to a vertebrate diet sooner in its development than …

Rapid Radiation Of Zeiformes Revealed Through Comparison Of Jaw Morphologies, Jeffrey Peters

Master's Theses

Zeiformes (e.g., Dories, Lookdown dories, Tinselfishes, Oreos) are a deep (1000 m) to mid-water (100 m) marine order of acanthomorph fishes with a circumpolar distribution, and a fossil record extending back to the Late Cretaceous. The order consists of 33 species across six families. The phylogenetic placement of the Zeiformes within Teleostei has been debated, but more recent studies based on both molecular and morphological data place Zeiforms within Paracanthopterygii closely related to Gadiformes. Zeiformes are characterized by anal and dorsal profiles that contain a variable number of fin rays and spines. They are mostly deep bodied, highly compressed fishes …

Biofilms On Plastic Litter: Community Composition And Activity And The Effects On Ecosystem Processes, Raul F. Lazcano

Master's Theses

The rapid increase of plastic production and disposal has resulted in plastic pollution becoming a global problem. In aquatic ecosystems, plastic litter is a substrate for biofilms, but little research has simultaneously assessed the effects of plastic litter on biofilm activity, community composition, and ecosystem processes. Our objectives were to: (1) measure biofilm activity and community composition on plastic litter relative to a natural surface in an urban river and (2) assess the impact of microplastic pollution on ecosystem metabolism and N2 flux in an oligotrophic lake. For objective (1) we incubated three common plastics with distinct physical and chemical …

Peruvian Mammals Collected By The Andrews University. Department Of Biology : Expeditions & Patterns Of Diversity, Kieran M. Taylor

Master's Theses

The Department of Biology at Andrews University conducted three expeditions to Peru in 1964, 1965, and 1968. During the expeditions, thousands of specimens were collected of several taxa, including mammals. As far as we know, no complete summary of the expeditions and the mammals collected has been created. Knowledge of the expeditions and evaluation of the mammals collected compared to current literature could have conservation implications. My purpose was to recreate the story of the expeditions and use this contextual information to catalog and identify the number of mammal species collected and evaluate the mammal diversity collected according to current …

Influences Of Grazing On Habitat Characteristics, Avian Community Composition And Nesting Bird Abundance Within Cheyenne Bottoms, Ks, Kirsten Granstrom-Arndt

Master's Theses

Cheyenne Bottoms is a 41,000-acre prairie-marsh ecosystem in central Kansas. Approximately 8,000 acres of mixed grassland are dedicated for the conservation of bird populations, but little is known about the status of bird communities within these areas. This study took place within grassland areas of Cheyenne Bottoms from May – July 2021. I investigated bird community composition, relative abundance of frequently observed bird species, vegetative characteristics, and similarity of sites across different grazing intensities (continuous, rotational, and non-grazed). The four most common bird species observed were dickcissel (Spiza americana), grasshopper sparrow (Ammodramus savannarum), meadowlark species ( …

A 16s & Its Soil Microbiome Analysis Of Native & Old-World Bluestem Invaded Soils Of Kansas Grasslands, Zachary Nelson

Master's Theses

Invasive species are becoming an increasing problem throughout the world. Their effect on local ecosystems is detrimental and widespread, harming productive efforts such as biomass accumulation, forcing native species (plant, animals, microbes) out, and ultimately, reducing biodiversity. Investigations of how invasive plants spread are widely studied; however, there has been little to no examination of how native plants influence the microbiome in the surrounding soil. In this study, I performed a fungal and bacterial metagenomic analysis of the soil and root microbiomes of both native grasslands and grasslands invaded by Old-World Bluestem Grasses to help determine if they influence the …

Healing Sanctuary, Kammy Downs

Master's Theses

The natural world has hidden wisdom and resources that are disregarded as we misuse our responsibility to care for them. The benefits of being in tune with nature have been traded for a culture that manipulates nature on the altar of expediency, waste, and unquenchable desire. Healing Sanctuary presents a duality. My work speaks about the relationship between the seen and unseen, represented by ten larger-than-life drawings of medicinal herbal plants that have had a profound physical healing effect on me. At the same time, plants, roots, and seeds create visceral metaphors for mental health. Our accomplishments, outlook on life, …

The Impact Of Planting Season And Crop Residue On Germination, Reproductive Success, And Mass Of Native Forbs, Michaela Vonlintel

Master's Theses

The lack of biodiversity in prairie restorations compared to native prairies is alarming, and restoring this diversity has been a key focus of research and restoration projects for years. This study aims to assess two variables: planting season and plant residue, for achieving success in forb establishment. This research was conducted in a greenhouse using mesocosms that were seeded in spring, summer, and fall with nine forb species. Half of each seasonal treatment received ground cover, while the other half did not. Two hypotheses were formed. The first was that the fall planting will be the most successful seasonal treatment …

Efficacy Of Non-Lethal Molecular Methods In Elucidating Distribution Of Gray Treefrog Complex (Hyla Chrysoscelis/Versicolor) In Kansas, Nora K. Lazerus

Master's Theses

Globally, amphibians are the most imperiled vertebrate taxa in part because they rely on both aquatic and terrestrial ecosystems. Specifically, their permeable skin makes them uniquely susceptible to habitat degradation and alteration. Cope’s Gray Treefrog (Hyla chrysoscelis) and the Gray Treefrog (Hyla versicolor) are a diploid-tetraploid, morphologically indistinguishable sister pair of cryptic anurans native to Kansas. Since 1987, the distribution of gray treefrogs in Kanas has extended west but the status of each species in the complex in Kansas is not known beyond its documented combined western expansion. Currently, species identification cannot be determined by nonlethal …

Effects Of Aggressive Reed (Phragmites Australis) On Aquatic Communities In A Kansas Reservoir, Allison Pardis

Master's Theses

Phragmites australis is an Aquatic Nuisance Species (ANS) in Kansas, a non-native reed that threatens lake and river ecology, displaces desirable species, impedes movement of wildlife and humans, that can have detrimental economic effects on communities. The majority of Phragmites research is in brackish ecosystems and the effects of Phragmites in freshwater systems and especially on fishes is undocumented, even as many states and agencies invest substantial resources in management. As freshwater systems face a biodiversity crisis, prevention and control of invasive species is critical. Effective management therefore requires a thorough understanding of the effects these invaders have on ecosystems …

A Comparison Of Impa2 & Isyna1 Gene Expression And Intracelular Myo-Inositol Levels In Bipolar And Non-Bipolar Disorder Derived Human Lymphoblasts, Christina Rosette

Master's Theses

The thesis objective was to determine if myo-inositol concentration levels and the gene expression of IMPA2 & ISYNA1 differ among bipolar disorder types 1 and 2 compared to healthy controls (non-bipolar disorder). Previous studies have correlated different myo-inositol concentration levels with specific phases of bipolar and pharmaceutical treatments. Two genes, inositol monophosphatase 2 (IMPA2) and inositol-3-phosphate synthase 1 (ISYNA1), involved in de novo myo-inositol biosynthesis have been implicated in the pathophysiology of bipolar. In this study, differences in myo-inositol concentration and IMPA2 & ISYNA1 gene expression in lymphoblasts derived from subjects with bipolar types 1, 2, and healthy control were …

Temozolomide-Nsaid Mixtures And Novel Diclofenac-Purine Hybrids As Potential Antiglioblastoma Agents, Joshua Rotich

Master's Theses

Glioblastoma (GBM) is the most common, lethal and aggressive brain tumor in adults. Standard treatment involves surgery, radiation therapy and temozolomide (TMZ) chemotherapy. However, GBM recurs and the average survival rate is between 12 to 18 months with 25% 1-year survival rate and 9% 5 years survival rate. Treatment options and advancement is limited by the blood brain barrier (BBB) which restricts drug entry into the brain and the immense heterogeneity of the tumor which limit adequate control of the entire tumor using one drug. In this research, we explored whether a combination mixture of TMZ and non-steroidal anti-inflammatory drugs …