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Characterization Of A Newly Cloned Dna Damage- Inducible Gene From Saccharomyces Cerevisiae Strain D43, June-Home Chen
Characterization Of A Newly Cloned Dna Damage- Inducible Gene From Saccharomyces Cerevisiae Strain D43, June-Home Chen
Master's Theses
The identification and characterization of DNA damage-inducible genes is an important topic in the study of the integrity of the cellular genome. Previously, a TnlO-lacZ derivative was inserted randomly into yeast cells and the resulting mutants were screened for insertions in DNA damage-inducible genes. S. cerevisiae strain D43 was identified as one such TnlO-lacZ insertion mutant. DNA sequencing results show the insertion in D43 is located within the TDH2 gene, which encodes glyceraldehyde-3-phosphate dehydrogenase. Treatment of strain D43 with DNA damaging agents and heat shock suggest TDH2 is responsive to DNA damage, but not to heat shock. Haploid mutants carrying …