Biology Commons^™

Is Selenoprotein K Required For Borrelia Burgdorferi Infection Within The Tick Vector Ixodes Scapularis?, Deepak Kumar, Monica Embers, Thomas N. Mather, Shahid Karim

Faculty Publications

Background: Tick selenoproteins are involved in regulating oxidative and endoplasmic reticulum stress during prolonged tick feeding on mammalian hosts. How selenoproteins are activated upon tick-borne pathogen infection is yet to be defined.

Methods: To examine the functional role of selenoprotein K in Borrelia burgdorferi infection within the tick host Ixodes scapularis, RNA interference (RNAi)-based gene silencing was performed.

Results: Selenoprotein K is an endoplasmic reticulum (ER)-resident protein and a component of the ERAD complex involved in ER homeostasis. A qRT-PCR assay revealed the significant upregulation of selenogene K (selenoK) expression in B. burgdorferi-infected …

The Kinesin‐3 Motor, Klp‐4, Mediates Axonal Organization And Cholinergic Signaling In Caenorhabditis Elegans, Margaret E. Magaletta, Kendall J. Perkins, Catherine P. Deuchler, Jay N. Pieczynski

Faculty Publications

Microtubule plus‐end directed trafficking is dominated by kinesin motors, yet kinesins differ in terms of cargo identity, movement rate, and distance travelled. Functional diversity of kinesins is especially apparent in polarized neurons, where long distance trafficking is required for efficient signal transduction‐behavioral response paradigms. The Kinesin‐3 superfamily are expressed in neurons and are hypothesized to have significant roles in neuronal signal transduction due to their high processivity. Although much is known about Kinesin‐3 motors mechanistically in vitro, there is little known about their mechanisms in vivo. Here, we analyzed KLP‐4, the Caenorhabditis elegans homologue of human KIF13A and KIF13B. …

Tidal And Meteorological Influences On The Growth Of Invasive Spartina Alterniflora: Evidence From Uav Remote Sensing, Xudong Zhu, Lingxuan Meng, Yihui Zhang, Qihao Weng, James T. Morris

Faculty Publications

Rapid invasion of Spartina alterniflora into Chinese coastal wetlands has attracted much attention. Many field and remote sensing studies have examined the spatio-temporal dynamics of S. alterniflora invasion; however, spatially explicit quantitative analyses of S. alterniflora invasion and its underlying mechanisms at both patch and landscape scales are seldom reported. To fill this knowledge gap, we integrated multi-temporal unmanned aerial vehicle (UAV) imagery, light detection and ranging (LiDAR)-derived elevation data, and tidal and meteorological time series to explore the growth potential (lateral expansion rates and canopy greenness) of S. alterniflora over the intertidal zone in a subtropical coastal wetland (Zhangjiang …

Longitudinal Homogenization Of The Microbiome Between Both Occupants And The Built Environment In A Cohort Of United States Air Force Cadets, Anukriti Sharma, Miles Richardson, Lauren Cralle, Christopher E. Stamper, Juan P. Maestre, Kelly A. Stearns-Yoder, Teodor T. Postolache, Katherine L. Bates, Kerry A. Kinney, Lisa A. Brenner, Christopher A. Lowry, Jack A. Gilbert, Andrew J. Hoisington

Faculty Publications

The microbiome of the built environment has important implications for human health and wellbeing; however, bidirectional exchange of microbes between occupants and surfaces can be confounded by lifestyle, architecture, and external environmental exposures. Here, we present a longitudinal study of United States Air Force Academy cadets (n = 34), which have substantial homogeneity in lifestyle, diet, and age, all factors that influence the human microbiome. We characterized bacterial communities associated with (1) skin and gut samples from roommate pairs, (2) four built environment sample locations inside the pairs’ dormitory rooms, (3) four built environment sample locations within shared spaces …

Tetrahydrocannabinol Reduces Hapten-Driven Mast Cell Accumulation And Persistent Tactile Sensitivity In Mouse Model Of Allergen-Provoked Localized Vulvodynia, Beebie Boo, Rohit Kamath, Erica Arriaga-Gomez, Jasmine Landry, Elizabeth Emanuel, Sookyong Joo, Marietta Sald Montivero, Tijana Martinov, Brian T. Fife, Devavani Chatterjea

Faculty Publications

Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. An increase in numbers of vulvar mast cells often accompanies a clinical diagnosis of vulvodynia and a history of allergies amplifies the risk of developing this condition. We previously showed that repeated exposures to oxazolone dissolved in ethanol on the labiar skin of mice led to persistent genital sensitivity to pressure and a sustained increase in labiar mast cells. Here we sensitized female mice to the hapten dinitrofluorobenzene (DNFB) dissolved in saline on their flanks, and subsequently challenged them with the same hapten or saline vehicle alone for ten …

Zinc Toxicity And Iron-Sulfur Cluster Biogenesis In Escherichia Coli, Huangen Ding

Faculty Publications

While zinc is an essential trace metal in biology, excess zinc is toxic to organisms. Previous studies have shown that zinc toxicity is associated with disruption of the (4Fe-4S] clusters in various dehydratases in Escherichia coli. Here, we report that the intracellular zinc overload in E. coli cells inhibits iron-sulfur cluster biogenesis without affecting the preassembled iron-sulfur clusters in proteins. Among the housekeeping iron-sulfur cluster assembly proteins encoded by the gene cluster iscSUA-hscBA-fdx-iscX in E. coli cells, the scaffold IscU, the iron chaperone IscA, and ferredoxin have strong zinc binding activity in cells, suggesting that intracellular zinc overload inhibits iron-sulfur …

Comparison Of Anthracnose Resistance With The Presence Of Two Scar Markers Associated With The Rca2 Gene In Strawberry, Melinda A. Miller-Butler, Barbara J. Smith, Brian R. Kreiser, Eugene K. Blythe

Faculty Publications

Strawberry anthracnose diseases are caused primarily by three Colletotrichum species: C. acutatum J.H. Simmonds, C. fragariae A.N. Brooks, and C. gloeosporioides (Penz.) Penz. & Sacc. Molecular markers are being used in breeding programs to identify alleles linked to disease resistance and other positive agronomic traits. In our study, strawberry cultivars and breeding germplasm with known anthracnose susceptibility or resistance to the three anthracnose-causing Colletotrichum species were screened for two sequence characterized amplified region (SCAR) markers linked to the Rca2 gene. The Rca2 resistant allele SCAR markers were associated with varying degrees of significance for a strawberry plant’s anthracnose resistance to …

Short-Term Effect Of Simulated Salt Marsh Restoration By Sand-Amendment On Sediment Bacterial Communities, François Thomas, James T. Morris, Cathleen Wigand, Stefan M. Sievert

Faculty Publications

Coastal climate adaptation strategies are needed to build salt marsh resiliency and maintain critical ecosystem services in response to impacts caused by climate change. Although resident microbial communities perform crucial biogeochemical cycles for salt marsh functioning, their response to restoration practices is still understudied. One promising restoration strategy is the placement of sand or sediment onto the marsh platform to increase marsh resiliency. A previous study examined the above- and below-ground structure, soil carbon dioxide emissions, and pore water constituents in Spartina alterniflora-vegetated natural marsh sediments and sand-amended sediments at varying inundation regimes. Here, we analyzed samples from the …

Ecological Mechanisms Can Modify Radiation Effects In A Key Forest Mammal Of Chernobyl, Tapio Mappes, Zbyszek Boratynski, Kati Kivisaari, Anton Lavrinienko, Anders P. Moller, Eugene Tukalenko, Phillip C. Watts

Faculty Publications

Nuclear accidents underpin the need to quantify the ecological mechanisms which determineinjury to ecosystems from chronic low-dose radiation. Here, we tested the hypothesis that ecological mech-anisms interact with ionizing radiation to affect natural populations in unexpected ways. We used large-scale replicated experiments and food manipulations in wild populations of the rodent, Myodes glareolus,inhabiting the region near the site of the Chernobyl disaster of 1986. We show linear decreases in breedingsuccess with increasing ambient radiation levels with no evidence of any threshold below which effects arenot seen. Food supplementation of experimental populations resulted in increased abundances but only inlocations where radioactive …

Algal-Mediated Priming Effects On The Ecological Stoichiometry Of Leaf Litter Decomposition: A Meta-Analysis, Halvor M. Halvorson, Steven N. Francoeur, Robert H. Findlay, Kevin A. Kuehn

Faculty Publications

In aquatic settings, periphytic algae exude labile carbon (C) that can significantly suppress or stimulate heterotrophic decomposition of recalcitrant C via priming effects. The magnitude and direction of priming effects may depend on the availability and stoichiometry of nutrients like nitrogen (N) and phosphorus (P), which can constrain algal and heterotrophic activity; in turn, priming may affect heterotrophic acquisition not only of recalcitrant C, but also N and P. In this study, we conducted a meta-analysis of algal-mediated priming across leaf litter decomposition experiments to investigate (1) bottom-up controls on priming intensity by dissolved N and P concentrations, and (2) …

Anisotropy Vs Isotropy In Living Cell Indentation With Afm, Yuri M. Efremov, Mirian Velay-Lizancos, Cory J. Weaver, Ahmad I. Athamneh, Pablo D. Zavattieri, Daniel M. Suter, Arvind Raman

Faculty Publications

The measurement of local mechanical properties of living cells by nano/micro indentation relies on the foundational assumption of locally isotropic cellular deformation. As a consequence of assumed isotropy, the cell membrane and underlying cytoskeleton are expected to locally deform axisymmetrically when indented by a spherical tip. Here, we directly observe the local geometry of deformation of membrane and cytoskeleton of different living adherent cells during nanoindentation with the integrated Atomic Force (AFM) and spinning disk confocal (SDC) microscope. We show that the presence of the perinuclear actin cap (apical stress fibers), such as those encountered in cells subject to physiological …

Genomic, Ecological, And Morphological Approaches To Investigating Species Limits: A Case Study In Modern Taxonomy From Tropical Eastern Pacific Surgeonfishes, Prosanta Chakrabarty

Faculty Publications

A wide variety of species are distinguished by slight color variations. However, molecular analyses have repeatedly demonstrated that coloration does not always correspond to distinct evolutionary histories between closely related groups, suggesting that this trait is labile and can be misleading for species identification. In the present study, we analyze the evolutionary history of sister species of Prionurus surgeonfishes in the Tropical Eastern Pacific (TEP), which are distinguished by the presence or absence of dark spots on their body. We examined the species limits in this system using comparative specimen-based approaches, a mitochondrial gene (COI), more than 800 nuclear loci …

Amplification Dynamics Of Platy-1 Retrotransposons In The Cebidae Platyrrhine Lineage, Mark A. Batzer, Jessica M. Storer, Jackson R. Mierl, Sarah A. Brantley

Faculty Publications

Platy-1 elements are Platyrrhine-specific, short interspersed elements originally discovered in the Callithrix jacchus (common marmoset) genome. To date, only the marmoset genome has been analyzed for Platy-1 repeat content. Here, we report full-length Platy-1 insertions in other New World monkey (NWM) genomes (Saimiri boliviensis, squirrel monkey; Cebus imitator, capuchin monkey; and Aotus nancymaae, owl monkey) and analyze the amplification dynamics of lineage-specific Platy-1 insertions. A relatively small number of full-length and lineage-specific Platy-1 elements were found in the squirrel, capuchin, and owl monkey genomes compared with the marmoset genome. In addition, only a few older Platy-1 subfamilies were recovered in …

The Cure For Cultivating Fastidious Microbes, William Wischusen, Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick

Faculty Publications

Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the …

The Strength Of Migratory Connectivity For Birds En Route To Breeding Through The Gulf Of Mexico, Emily B. Cohen, Clark R. Rushing, Frank R. Moore, Michael T. Hallworth, Jeffrey A. Hostetler, Mariamar Gutierrez Ramirez, Peter P. Marra

Faculty Publications

The strength of migratory connectivity is a measure of the cohesion of populations among phases of the annual cycle, including breeding, migration, and wintering. Many Nearctic‐Neotropical species have strong migratory connectivity between breeding and wintering phases of the annual cycle. It is less clear if this strength persists during migration when multiple endogenous and exogenous factors may decrease the cohesion of populations among routes or through time along the same routes. We sampled three bird species, American redstart Setophaga ruticilla, ovenbird Seiurus aurocapilla, and wood thrush Hylocichla mustelina, during spring migration through the Gulf of Mexico region …

Ultra-Strong Long-Chain Polyamide Elastomers With Programmable Supramolecular Interactions And Oriented Crystalline Microstructures, Lingzhi Song, Tianyu Zhu, Liang Yuan, Jiangjun Zhou, Yaqiong Zhang, Zhongkai Wang, Chuanbing Tang

Faculty Publications

Polyamides are one of the most important polymers. Long-chain aliphatic polyamides could bridge the gap between traditional polyamides and polyethylenes. Here we report an approach to preparing sustainable ultra-strong elastomers from biomass-derived long-chain polyamides by thiol-ene addition copolymerization with diamide diene monomers. The pendant polar hydroxyl and non-polar butyrate groups between amides allow controlled programming of supramolecular hydrogen bonding and facile tuning of crystallization of polymer chains. The presence of thioether groups on the main chain can further induce metal–ligand coordination (cuprous-thioether). Unidirectional step-cycle tensile deformation has been applied to these polyamides and significantly enhances tensile strength to over 210 …

Functional And Transcriptomic Characterization Of Carboplatin-Resistant A2780 Ovarian Cancer Cell Line, Tamara Viscarra, Kurt Buchegger, Ignacio Jofre, Ismael Riquelme, Louise Zanella, Michel Abanto, Alyssa C. Parker, Stephen R. Piccolo, Juan Carlos Roa, Carmen Ili, Priscilla Brebi

Faculty Publications

Background

Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen is based on carboplatin (CBDCA). However, CBDCA resistance is the main obstacle to a better prognosis. An in vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize cellular and molecular changes of induced CBDCA-resistant ovarian cancer cell line A2780.

Methods

The cell selection strategy used in this study was a dose-per-pulse method using a concentration of 100 μM for 2 h. Once 20 cycles of exposure to …

Complex Gene Loss And Duplication Events Have Facilitated The Evolution Of Multiple Loricrin Genes In Diverse Bird Species, Anthony C. Davis, Matthew J. Greenwold, Roger H. Sawyer

Faculty Publications

The evolution of a mechanically resilient epidermis was a key adaptation in the transition of amniotes to a fully terrestrial lifestyle. Skin appendages usually form via a specialized type of programmed cell death known as cornification which is characterized by the formation of an insoluble cornified envelope (CE). Many of the substrates of cornification are encoded by linked genes located at a conserved genetic locus known as the epidermal differentiation complex (EDC). Loricrin is the main protein component of the mammalian CE and is encoded for by a gene located within the EDC. Recently, genes resembling mammalian loricrin, along with …

The Aqueous Environment As An Active Participant In The Protein Folding Process, Michal Brylinski

Faculty Publications

Existing computational models applied in the protein structure prediction process do not sufficiently account for the presence of the aqueous solvent. The solvent is usually represented by a predetermined number of H2O molecules in the bounding box which contains the target chain. The fuzzy oil drop (FOD) model, presented in this paper, follows an alternative approach, with the solvent assuming the form of a continuous external hydrophobic force field, with a Gaussian distribution. The effect of this force field is to guide hydrophobic residues towards the center of the protein body, while promoting exposure of hydrophilic residues on its surface. …

Host Lipids In Positive-Strand Rna Virus Genome Replication, Zhenlu Zhang, Guijuan He, Natalie A. Filipowicz, Glenn Randall, George A. Belov, Benjamin G. Kopek, Xiaofeng Wang

Faculty Publications

Membrane association is a hallmark of the genome replication of positive-strand RNA viruses [(+)RNA viruses]. All well-studied (+)RNA viruses remodel host membranes and lipid metabolism through orchestrated virus-host interactions to create a suitable microenvironment to survive and thrive in host cells. Recent research has shown that host lipids, as major components of cellular membranes, play key roles in the replication of multiple (+)RNA viruses. This review focuses on how (+)RNA viruses manipulate host lipid synthesis and metabolism to facilitate their genomic RNA replication, and how interference with the cellular lipid metabolism affects viral replication.

Deepdrug3d: Classification Of Ligand-Binding Pockets In Proteins With A Convolutional Neural Network, Michal Brylinski, Limeng Pu, Rajiv Gandhi Govindaraj, Jeffrey Mitchell Lemoine

Faculty Publications

Comprehensive characterization of ligand-binding sites is invaluable to infer molecular functions of hypothetical proteins, trace evolutionary relationships between proteins, engineer enzymes to achieve a desired substrate specificity, and develop drugs with improved selectivity profiles. These research efforts pose significant challenges owing to the fact that similar pockets are commonly observed across different folds, leading to the high degree of promiscuity of ligand-protein interactions at the system-level. On that account, novel algorithms to accurately classify binding sites are needed. Deep learning is attracting a significant attention due to its successful applications in a wide range of disciplines. In this communication, we …

Host Sex, Size, And Hemoparasite Infection Influence The Effects Of Ectoparasitic Burdens On Free-Ranging Iguanas, Christine Lattin

Faculty Publications

Investigations focusing on host-ectoparasite interactions in animals have revealed asymptomatic to severe health and fitness consequences suggesting that species mobilize different interspecific response mechanisms. Fewer studies, however, have examined intraspecific responses to ectoparasitic burdens. In this study, we analyzed host health and fitness responses to increasing ectoparasite burdens along with the presence/absence of hemoparasites of free-ranging insular rock iguanas (Cyclura cychlura) in The Bahamas. Using hematology, plasma biochemistry, as well as body condition and growth rate comparisons, we failed to find significant associations of tick burdens with annual growth rate, corticosterone, packed cell volume, total white blood cell, and heterophil, …

Predator-Prey Dynamics Of Bald Eagles And Glaucous-Winged Gulls At Protection Island, Washington, Usa, Shandelle M. Henson, Robert A. Desharnais, Eric T. Funasaki, Joseph G. Galusha, James W. Watson, James L. Hayward

Faculty Publications

1. Bald eagle (Haliaeetus leucocephalus) populations in North America rebounded in the latter part of the twentieth century, the result of tightened protection and outlawing of pesticides such as DDT. An unintended consequence of recovery may be a negative impact on seabirds. During the 1980s, few bald eagles disturbed a large glaucous‐winged gull (Larus glaucescens) colony on Protection Island, Washington, USA, in the Salish Sea. Breeding gull numbers in this colony rose nearly 50% during the 1980s and early 1990s. Beginning in the 1990s, a dramatic increase in bald eagle activity ensued within the colony, after which began a significant …

Exposure To Environmental Radionuclides Associates With Tissue-Specific Impacts On Telomerase Expression And Telomere Length, Jenni Kesaniemi, Anton Lavrinienko, Eugene Tukalenko, Zbyszek Boratynski, Kati Kivisaari, Tapio Mappes, Gennadi Milinevsky, Anders Pape Moller, Timothy A. Mousseau, Phillip C. Watts

Faculty Publications

Telomeres, the protective structures at the ends of chromosomes, can be shortened when individuals are exposed to stress. In some species, the enzyme telomerase is expressed in adult somatic tissues, and potentially protects or lengthens telomeres. Telomeres can be damaged by ionizing radiation and oxidative stress, although the effect of chronic exposure to elevated levels of radiation on telomere maintenance is unknown for natural populations. We quantified telomerase expression and telomere length (TL) in different tissues of the bank vole Myodes glareolus, collected from the Chernobyl Exclusion Zone, an environment heterogeneously contaminated with radionuclides, and from uncontaminated control sites …

Potential New Biomarkers For Endometrial Cancer, Michelle H. Townsend, Zac E. Ence, Abigail M. Felsted, Alyssa C. Parker, Stephen R. Piccolo, Richard A. Robison, Kim L. O'Neill

Faculty Publications

Abstract

Background: Incidence of endometrial cancer are rising both in the United States and worldwide. As endometrial cancer becomes more prominent, the need to develop and characterize biomarkers for early stage diagnosis and the treatment of endometrial cancer has become an important priority. Several biomarkers currently used to diagnose endometrial cancer are directly related to obesity. Although epigenetic and mutational biomarkers have been identified and have resulted in treatment options for patients with specific aberrations, many tumors do not harbor those specific aberrations. A promising alternative is to determine biomarkers based on differential gene expression, which can be used to …

Effects Of Substrate On Movement Patterns And Behavior Of Stream Fish Through Culverts: An Experimental Approach, Kyla Johnson, Lindsay E. Wait, Suzanne K. Monk, Russell Rader, Rollin H. Hotchkiss, Mark C. Belk

Faculty Publications

Culverts can provide a significant barrier to fish passage by fragmenting fish habitats and impeding the passage success of small-bodied fish. Geographical connectivity is critical to the maintenance of diverse fish assemblages. Culverts with high cross-sectional velocity can cause population fragmentation by impeding passage of small, freshwater fish. Behavioral responses of small fish to high velocities can differ among functional groups, and swimming behavior of many species is not well known. We tested effects of substrate type on swimming behavior in two small, freshwater fish species—southern leatherside chub (Lepidomeda aliciae, a midwater species), and longnose dace (Rhinichthys …

Diversity And Shifts Of The Bacterial Community Associated With Baikal Sponge Mass Mortalities, Sergei Belikov, Natalia Belkova, Tatiana Butina, Lubov Chernogor, Alexandra Martynova-Van Kley, Armen Nalian, Colin Rorex, Igor Khanaev, Olga Maikova, Sergey Feranchuk

Faculty Publications

The disease of freshwater sponges was first discovered in 2011, when pink samples were found in the Central Basin of Lake Baikal. Subsequently, the visible signs of the disease have changed, and now sponges appear with various symptoms of damage to the body, such as discoloration, tissue necrosis, the formation of brown patches and dirty-purple biofilms on some branches. These signs of the disease are accompanied by the mass death of sponges. We identified differences in microbiomes by sequencing 16S rRNA genes and found changes in the consortium of microorganisms of freshwater Baikal sponges. We found that the observed imbalance …

Structural And Functional Determinants Of Rodent And Human Surfactant Protein A: A Synthesis Of Binding And Computational Data, Armen Nalian, Todd M. Umstead, Ching-Hui Yang, Patricia Silveyra, Neal J. Thomas, Joanna Floros, Francis X. Mccormack, Zissis C. Chroneos

Faculty Publications

Surfactant protein A (SP-A) provides surfactant stability, first line host defense, and lung homeostasis by binding surfactant phospholipids, pathogens, alveolar macrophages (AMs), and epithelial cells. Non-primates express one SP-A protein whereas humans express two: SP-A1 and SP-A2 with core intra- and inter-species differences in the collagen-like domain. Here, we used macrophages and solid phase binding assays to discern structural correlates of rat (r) and human (h) SP-A function. Binding assays using recombinant rSP-A expressed in insect cells showed that lack of proline hydroxylation, truncations of amino-terminal oligomerization domains, and site-directed serine (S) or alanine (A) mutagenesis of cysteine 6 (C6S), …

Whole Leaf Photosynthetic Light Response In A Carnivorous Plant Species Sarracenia Alata, K. A. Hopkins, Dennis A. Gravatt

Faculty Publications

Photosynthetic light-response curves of mature leaves of Sarracenia alata were generated and characterized using a custom built chamber (2.79 L) capable of accommodating whole leaves, or pitchers, coupled to a LI-COR 6400XT Portable Photosynthesis System. In addition to generating light-response curves, chlorophyll content, leaf area, and dry mass measurements were obtained for each pitcher. Gas-exchange variables from the light-response curves are reported on a leaf area and dry mass basis. Calculated light-saturated photosynthesis was found to be 2.09 µmol(CO₂) m^-2 s^-1 with a light-saturation point of 315.14 µmol(photon) m^-2 s^-1, light-compensation point of …

Arachidin-1 And Arachidin-3 Modulation Of Rotavirus-Infected Ma104 Cells, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah N. Lockwood, Macie N. Mattila, Stormey B. Wisdom, Luanna L. Saade Ferreira, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball, Rebecca D. Parr

Faculty Publications

Rotavirus (RV) causes severe life-threatening diarrhea in young children and immunocompromised individuals. There are several licensed attenuated vaccines for young children, but there are no vaccines or antiviral therapeutics for immunocompromised patients of any age. Previously, our laboratory demonstrated that arachidin 1 (A1) and arachidin 3 (A3) decreases the number of infectious simian RV particles and RV non-structural protein 4 (NSP4) in a human intestinal cell line which suggests effects on RV replication. This study examined the effects of the arachidins on the human RV (Wa)-infected African green monkey kidney cell line, MA104. The addition of either A1 or A3 …