Biology Commons^™

Characterization Of Microbial Communities In Karstic Sediments : A Study Of Cave Bacteria In Central Kentucky., Justine Kay Roback 1964-

Electronic Theses and Dissertations

Few previous studies have compared microbial communities in subterranean and surface environments. Chemical analyses used to characterize the surface and cave microbial environments indicated limited exchange between surface and subsurface waters. Here, bacterial communities from four distinct cave sediments collected in central Kentucky and adjacent surface streams were compared using a combination of culture and molecular methods. Predictions were that: 1) Cave communities will have unique community structures when compared to those of nearby surface sediments, and 2) Genetic diversity of bacterial communities in cave sediments will be higher than those in nearby surface sediments. Since diversity and community structure …

Effects Of Food Deprivation On Blood Lipid Concentration And Composition In Steller Sea Lions (Eumetopias Jubatus), Michelle Lea Berman

Electronic Theses and Dissertations

Steller sea lions, the largest Otariid, fast during their breeding season; during this time they refrain from ingesting food for a period of 12-43 days. Fasting, while undertaking an extremely energetically demanding activity (breeding and pupping), requires specific physiological adaptations. This study examined the physiological response to fasting of two age classes, juveniles and sub-adults, during the breeding and non-breeding seasons to determine how these animals utilize lipids and the pattern of fatty acid mobilization from lipid stores during fasting. Four juveniles and 5 sub-adults were fasted for one and two weeks, respectively, and blood samples were collected approximately every …

Responses Of The Florida Mouse (Podomys Floridanus) To Habitat Management, Jason Depue

Electronic Theses and Dissertations

The Florida mouse (Podomys floridanus), a species restricted to the Lake Wales Ridge and the Atlantic Coastal Ridge, is recognized by the Florida Fish and Wildlife Conservation Commission as a species of special concern, highlighting its status as a species that is threatened by habitat loss statewide. Publicly owned lands offer protection for the species, but management is generally focused on protecting biodiversity in general and not a particular species. The response of the Florida mouse to land management practices such as mechanical treatment and prescribed fires is poorly documented. This research examined the population responses of Florida mice on …

The Effects Of Ecotourism On Polar Bear Behavior, Gillian Eckhardt

Electronic Theses and Dissertations

Polar bears spend the majority of their lives on the sea ice, where they gain access to seals and mates. In western Hudson Bay, the sea ice melts for three to four months in the summer, and polar bears there are forced onto land. These bears live on their fat reserves for the duration of the iceless period, until temperatures get colder in the fall and freeze up begins. The aggregation of polar bears near Churchill, Manitoba during the ice free period has led to a thriving tourist industry, with a large influx of tourists visiting Churchill in the fall …

Site Preparation Methods For Restoration Of Non-Native Pasturelands To Native Upland Habitat, Krisann Joie Kosel

Electronic Theses and Dissertations

The flatwoods ecosystem of Florida has been heavily depleted over time but remains one of the most important systems to many threatened and endangered species. Areas that have been converted into non-native pastures may be restored to provide not only this invaluable ecosystem but also restore connectivity of the surrounding ecosystems. The pasture areas on The Disney Wilderness Preserve in central Florida were surveyed, and a conceptual plan for restoration was written in 1996. That same year a pilot study was developed to assess five methods for removing non-native pasture grasses. The treatments studied were single herbicide, single disc, multiple …

A Theoretical Approach To Assessing Annual Energy Balance In Gray Whales (Eschrichtius Robustus), Nathalie Lucie Elizabeth Greenwald

Electronic Theses and Dissertations

While direct measurements of energetic demands are nearly impossible to collect on large cetaceans, comprehensive bioenergetic models can give insights on such parameters by combining physiological and ecological knowledge. This model was developed to estimate necessary food intake of gray whales, Eschrichtius robustus, of the Eastern North Pacific stock. Field Metabolic Rates (FMR) for gray whales were first estimated based on various assumptions (e.g. volumetric representation of gray whales, extent of their feeding season, and blubber depth distribution) using morphometric data, energetic costs, and food assimilation according to age and gender specific requirements. Food intake rates for gray whales of …

Historical Responses Of Marine Turtles To Global Climate Change And Juvenile Loggerhead Recruitment In Florida, Joshua Reece

Electronic Theses and Dissertations

Marine turtle conservation is most successful when it is based on sound data incorporating life history, historical population stability, and gene flow among populations. This research attempts to provide that information through two studies. In chapter I, I identify historical patterns of gene flow, population sizes, and contraction/expansion during major climatic shifts. In chapter II, I reveal a life history characteristic of loggerhead turtles previously undocumented. I identify a pattern of juvenile recruitment to foraging grounds proximal to their natal nesting beach. This pattern results in a predictable recruitment pattern from juvenile foraging ground aggregations to local rookeries. This research …

Native And Invasive Competitors Of The Eastern Oyster Crassostrea Virginica In Mosquito Lagoon, Florida, Michelle Boudreaux

Electronic Theses and Dissertations

Populations of Crassostrea virginica within Mosquito Lagoon, Florida have recently undergone significant die-offs, which are a subject of major concern. Restoration efforts within Mosquito Lagoon are focusing on reconstructing the three-dimensional reef habitats. Before effective protocols can be established, however, important questions about the sources of juvenile and adult oyster mortality must be answered. Potential causes of Crassostrea virginica mortality in the Indian River Lagoon system include sediment loads, competition, predation, and disease. My research focused on the interactions between oysters and the competitors that may affect the settlement, growth, and survival of Crassostrea virginica. The four objectives of my …

Soil Microbial Community Dynamics In Florida Scrub Ecosystem, Maria Albarracin

Electronic Theses and Dissertations

Pyrogenic ecosystems are maintained by fires which vary in frequency, seasonality, and intensity. Florida oak-saw palmetto scrub ecosystem is characterized by fires occurring at intervals of 10-20 years. Diverse factors as private land acquisition and development has created a patchy distribution of scrub ecosystems and also interrupted the natural fire cycle. The effects of fire over plant regeneration and fauna habitat utilization of the scrub have been well characterized in previous research. In the present paper the objective is to characterize the short- and long-term fire effects on the soil microbial community. Fire effects were studied in a chronosequence, comprising …

Effects Of A Shore Protection Project On Loggerhead And Green Turtle Nesting Activity And Reproduction In Brevard County, Florida, Kelly Brock

Electronic Theses and Dissertations

Marine turtle reproductive success is strongly correlated with the stability and quality of the nesting environment. Because females show fidelity to key nesting beaches, the management and physical characteristics of these beaches directly affect future generations of marine turtles and may be essential for the recovery of these threatened and endangered species. The impacts of beach restoration on loggerhead turtles (Caretta caretta) and on green turtles (Chelonia mydas) were investigated. Previous studies concerning beach nourishment projects have focused on loggerhead turtles. I compared data between nourished and non-nourished areas and between loggerhead and green turtles. I found, at one season …

Expression Of Gal/Galnac Lectin Of Entamoeba Histolytica In Transgenic Chloroplasts To Develop A Vaccine For Amebiasis, Seethamahalakshmi Chebolu

Electronic Theses and Dissertations

Amebiasis, also defined as invasive intestinal and extra intestinal amebiasis, is caused by Entameoba histolytica, an invasive protozoan parasite. World Health Organization (WHO) has reported that approximately 50 million people are infected each year causing an estimated 40 to 100 thousand deaths annually. Entameoba histolytica ranks only second to malaria as a protozoan cause of death. Amebiasis occurs world wide but people living in Central and South America, Africa and Asia are the majority to suffer from morbidity and mortality. The enteric parasite has no zoonotic reservoirs and insect vectors for its transmission and infects humans and non-human primates. Therefore, …

Juvenile Green Turtle (Chelonia Mydas) Foraging Ecology:Feeding Selectivity And Forage Nutrient Analysis, Eliza Gilbert

Electronic Theses and Dissertations

For the endangered green turtle, Chelonia mydas, a fundamental component of recovery and conservation is an understanding of its foraging ecology. Foraging optimality models suggest animals will select resources of high quality over those of low quality. For green turtles, this behavior is important, as sufficient quantities of nutritionally adequate forage items are necessary for growth and reproduction. One intrinsic element in the understanding of green turtle foraging ecology is to identify and document the availability and quality of forage resources preferred by green turtles. The objectives of this study were: 1) determine whether juvenile green turtles showed a feeding …

Cascading Effects Of Predator Removal On The Ecology Of Sea Turtle Nesting Beaches, Brandon Barton

Electronic Theses and Dissertations

Traditional views of food web dynamics have characterized species interactions as linear and direct. However, modern food web theory suggests that interactions can also be nonlinear and indirect, so that disturbance at one trophic level is transmitted throughout the community. Many previous studies have demonstrated that the removal of top predators from terrestrial ecosystems can have broad-scale impacts on community ecology. I examined the direct and indirect effects of raccoon removal from sea turtle nesting beaches in east-central Florida during the summers of 2003 and 2004. Raccoon and ghost crab predation are among the highest causes of egg mortality for …

Confocal Laser Scanning Microscopy As A Tool For The Investigation Of Tetracycline Fluorescence In Archaeologicalhuman Bone, Corey Maggiano

Electronic Theses and Dissertations

Fluorochromes such as tetracycline have been used to label bone for histomorphometric analysis, measuring bone formation, growth, maintenance, and pathology. More recently, similar fluorescence has been observed in ancient human bone. Attributed to tetracycline (TC) exposure, this phenomenon could affect various aspects of health during life and/or preservation of remains postmortem. Standard epifluorescence microscopy is the most common tool employed in the analysis of these labels. Though valuable, this technique is limited by its inability to penetrate bone three-dimensionally and its inclusion of out-of-focus light, possibly disrupting accurate analysis. Confocal Laser Scanning Microscopy (CLSM) has been demonstrated as a valuable …

Characterization Of A Catechol-Type Siderophore And The Detection Of A Possible Outer Membrane Receptor Protein From Rhizobium Leguminosarum Strain Iari 312., Brianne Lee Clark

Electronic Theses and Dissertations

Many gram-negative bacteria produce and secrete siderophores under iron-deficient conditions. Siderophores are low molecular weight compounds (600-1500 Daltons), which chelate ferric iron with an extremely high affinity, and the complex is actively transported across the outer and inner membranes of gram-negative bacteria. There are two main classes of siderophores: catechol and hydroxamate. Catechol-type siderophores chelate ferric iron via hydroxyl groups, and hydroxamate-type siderophores chelate ferric iron via a carbonyl group with an adjacent nitrogen. Rhizobia fix atmospheric nitrogen symbiotically in leguminous plants using the iron-containing enzyme nitrogenase. To satisfy their iron requirements, many rhizobia are known to produce siderophores. Rhizobium …

The Magnolia Warbler Dendroica Magnolia On Unaka Mountain, Unicoi County, Tn: Possible Breeding And Habitat Analysis., Kevin Patrick Elam

Electronic Theses and Dissertations

The chief purpose of this study is to provide information on the habitat and breeding information of Magnolia Warblers on Unaka Mountain. Magnolia Warblers breed in Canada and the Northeastern United States. There are no current breeding records for this species in Tennessee.

For the habitat analysis, trees were identified on individual subplots. Shrubs were analyzed using the point-intercept method. Most of the major tree and shrub species were different than those found in Maine, which is a major breeding region for this species. Therefore, it's the structure of the habitat that is important for nesting. Fledglings were seen, giving …

Population Size And Movements Of Spotted Salamanders At South Holston Dam, Sullivan County, Tennessee., Eric Alexander Smith

Electronic Theses and Dissertations

In the Southern Appalachians, there are growing concerns about Spotted Salamanders (Ambystoma maculatum) due to habitat destruction and the uncertain future of their forested riparian habitat. In this study, I establish baseline data of a population of Spotted Salamanders so future monitoring of this population can be performed. The study site is located in northeastern Tennessee, just east of the city of Bristol. It is a vernal pond at the Osceola Island Recreation Area one mile below the South Holston Dam on Holston View Dam road. A drift fence was established completely around the perimeter of the vernal …

Interactions Between The Nudibranch Okenia Zoobotryon And Its Bryozoan, Nicole Robinson

Electronic Theses and Dissertations

In the Indian River Lagoon, the nudibranch Okenia zoobotryon and its egg masses are found attached to the bryozoan Zoobotryon verticillatum throughout the year. Okenia zoobotryon is thought to live, feed, and reproduce exclusively on Z. verticillatum, which would make this a very specialized predator-prey interaction. The primary goal of my study was to document certain aspects of the ecological and chemical relationships between Z. verticillatum and O. zoobotryon. Specifically, I wanted to understand the cues used by the nudibranch to locate and remain on Z. verticillatum. Population surveys on Z. verticillatum, as well as other potential hosts, were performed. …

The Ecology Of Drift Algae In The Indian River Lagoon, Florida, Julie Lynnae Liss

Electronic Theses and Dissertations

To gain an understanding of the ecology of drift algae in the Indian River Lagoon system along the east coast of central Florida, four questions were addressed: 1) What is the composition and rate of accumulation of drift? 2) How much movement and turnover occurs within drift accumulations? 3) Do growth rates differ for drift versus attached algae? 4) Is there a difference in photosynthetic performance in drift versus attached algal species? Manipulative field and laboratory experiments were conducted to address these questions with the green macroalga Codium decorticatum and the red macroalga Gracilaria tikvahiae. Changes in pigment concentration and …

Land Use Effects On Lake Water Quality In Central Florida, Jonathan Seiler

Electronic Theses and Dissertations

Land use affects the water quality of lakes. Different land use types yield different effects due to varying amounts and constituents of runoff. In this study, the effects of surrounding land use on the water quality of 50 lakes in Seminole County, Florida was assessed. Using GIS, I placed buffers of 100 and 500 m around each lake. The percentages of land use type were calculated within these buffers for 1990 and 1995. An ordination of lakes was done using Canonical Correspondence Analysis (CCA) to determine if the surrounding land use patterns were adequate to describe the trophic status of …

Recruitment And Restoration Of The Oyster Crassostrea Virginica In Areas With Intense Boating Activity In Mosquito Lagoon, Florida, Lisa Michele Wall

Electronic Theses and Dissertations

Productivity, diversity and survival of estuaries are threatened by explosive coastal population growth and associated recreational activities. One major area of recreational growth has been the number of small pleasure craft motoring in shallow waters at high speeds. On the east coast of Central Florida in the Indian River Lagoon system, intense boating activity occurs year-round and intertidal reefs of the eastern oyster Crassostrea virginica with dead margins (piles of disarticulated shells) on their seaward edges are commonly found adjacent to major boating channels. The cause(s) of the dead margins is unclear. However, the disarticulated shells may be reducing reef …

Genetic Analysis Of Rhoa Signaling During Epithelial Morphogenesis In Drosophila, Amanda Fitch Leppert

Electronic Theses and Dissertations

Epithelial morphogenesis is contingent upon cell shape changes. Cell shape changes are the driving force for the metamorphosis of the adult Drosophila leg from the leg imaginal disc precursor. Genetic analysis has identified several Drosophila genes involved in regulating cell shape changes during leg disc morphogenesis. These include members of the RhoA signaling pathway and the product of the Stubble-stubbloid (Sb-sbd) locus, a transmembrane serine protease. Mutations in the Sb-sbd gene interact genetically with the members of the RhoA signaling pathway, however the nature of the relationship between Sb-sbd serine protease activity and RhoA signaling is not understood. To identify …

Habitat Use And Seasonal Activity Of Selected Snakes On John F. Kenned, Karen Dyer

Electronic Theses and Dissertations

An intensive, replicated monthly sampling of snake communities inhabiting four habitat types was conducted at John F. Kennedy Space Center, Brevard County, Florida from November 2002 through October 2003. Thirteen species (580 individuals plus 74 recaptures) plus one hybrid were captured. The three most commonly captured species, Coluber constrictor, Thamnophis sirtalis, and Thamnophis sauritus, combined made up 85% of the sample. These three species were active during every month of the year, but showed modal activity patterns typical of Temperate Zone snakes. Monthly snake captures were correlated with monthly captures of potential prey species and with mean monthly temperature. Species …

A Comparison Of Bioaccumulation And Digestive Enzyme Solubilization Of Copper In Two Species Of Sea Cucumbers With Different Feeding Habits., John W. Bundridge

Electronic Theses and Dissertations

The mode of feeding exhibited by different organisms may influence the form or quantity of copper that is bioaccumulated. This hypothesis has been tested by exposing 2 species of sea cucumbers, Pentacta anceps and Stichopus chloronotus, which possess different feeding modes, to varying concentrations of copper.

The digestive tract and body wall were dissected and analyzed for copper concentration using atomic absorption spectroscopy. A trend was present, exhibiting a small dose dependent curve. The results did not show a significant difference between species or treatments. This study indicates that feeding mode may influence the amount of copper accumulated but …

Cloning And Sequencing Of Glucosyltransferase (Gt) Genes From Grapefruit (Citrus Paradisi) Seedlings, Searching For 7-O-Gt., Mebrahtu Berhane Sibhatu

Electronic Theses and Dissertations

Flavonoids play crucial roles in plant life cycles and in human welfare. Grapefruit plants produce several glycosylated flavonoids and the glycosylation reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative grapefruit flavonoid GTs. Gene specific primers were designed from the plant secondary product glucosyltransferase (PSPG) box and used to amplify 5’GT clones. Clone-specific primers were designed from 5’clones to amplify 3’ GT clones. We obtained 5 5’ clones and 1 3’ clone as candidate GTs. We used 3 of these clones to predict 2 compiled GT …

Host Defense Mechanisms In The Crayfish: The Effect Of Injection With Live Or Killed Bacteria., Kimberly R. Goins

Electronic Theses and Dissertations

An increase in attachment of SRBCs to Procambarus clarkii hemocytes has been shown after the crayfish were injected with a live or killed Pseudomonas strain RS2b. The increase in attachment occurred at 8 hours post injection and peaked at 24 hours for both experimental groups. The population of hemocytes with receptors for LPS and mannose also increased at 8 hours post injection and peaked at 24 hours for both experimental groups. At 96 hours post injection the number of receptor bearing hemocytes and hemocytes bound to SRBCs began to decrease to the level of the control for both groups. The …

Fire, Soil, Native Species And Control Of Phalaris Arundinacea In A Wetland Recovery Project., Richard Douglas Foster

Electronic Theses and Dissertations

Southern Appalachian Phalaris arundinacea control was investigated by: 1) correlating cover and species richness with soil characteristics across transects; 2) burning and herbicide use to determine conditions facilitating native plant establishment; and 3) hemi-parasitic Pedicularis lanceolata tested as a biological control.

Phalaris cover was correlated with subsoil consolidation; areas without Phalaris had consolidated subsoil while Phalaris at >50% cover established on loose soil. Phalaris cover inhibited species richness (r²=0.78). No soil characteristic predicted species richness.

Herbicide reduced Phalaris cover and aerial biomass by 23% and 63% respectively, compared to controls. Burning was ineffective. Two summers after herbicide Phalaris …

The Effects Of Prescribed Fire On Millipede And Salamander Populations In A Southern Appalachian Deciduous Forest., Alison Baird Gagan

Electronic Theses and Dissertations

Prescribed fire has increased as a forest management practice in southern Appalachia, but investigations into the effects of this silvicultural treatment on non-game wildlife inhabiting the region is limited. This study investigated the effects of prescribed fire on millipede and salamander populations. Seventeen sites within the Cherokee National Forest in east Tennessee that were treated once with prescribed fire between 1998 and 2002 were examined in the spring of 2002. Each burned plot was paired with an adjacent unburned plot.

The number of individual millipedes and salamanders collected from burned plots was compared to the corresponding control plot. Millipedes declined …

An Experimental Approach To Teaching The Concept Of Functional Diversity., Cory Mckelvey Stanley

Electronic Theses and Dissertations

This study tested an experimental approach to use in teaching the concept of functional diversity. The project culminated in a laboratory exercise for use in high schools.

Experimental design consisted of representatives of three functional groups of plants, (legumes, grasses, and forbs), planted singly, and in two, or three species combinations. Legumes were represented by Trifolium repens and Medicago lupulina, grasses were represented by Cynodon dactylon and Festuca rubra, and forbs were represented by Helianthus annus and Raphanus sativa.

Plants were grown inside a controlled growth chamber. During the growth phase, measurements were taken to highlight temporal …

Mannose And Lipopolysaccharide Receptors On The Surface Of Granular Hemocytes From The Crayfish Procambarus Clarkii., Gelu Dobrescu

Electronic Theses and Dissertations

Procambarus clarkii hemocytes have been shown to bind to both LPS and a mannose containing neoglycoprotein conjugated to fluorescent probes. A decrease in the observed number of fluorescent hemocytes indirectly indicated that the binding of FITC-LPS to these cells is impaired by initial incubation with LPS. Prior treatment of hemocytes with horseradish peroxidase, a mannose rich glycoprotein, decreased their capacity to bind mannose. Mannan had no inhibitory effect on the binding of either ligand. Mixed hemocytes and hemocyte subpopulations separated by buoyant density were incubated with both ligands and revealed that a subpopulation of granular hemocytes bound both LPS and …