Biology Commons^™

Effect Of Body Size On Expression Of Manduca Sexta Midgut Genes, Christopher Gillen

Christopher M. Gillen

Isometric growth of larval insect midgut predicts that the ratio of midgut surface area to body mass decreases as larvae grow. Gut tissue and gut content masses were measured in first through fifth instar Manduca sexta larvae. Wet mass of gut tissue increased in relationship to body mass with a scaling exponent of 0.85 compared to an exponent of 1.33 for gut content mass, suggesting that surface area becomes increasingly limiting in larger larvae. To test the hypothesis that compensation for the decrease in relative surface area of the midgut occurs by increased expression of membrane proteins, we compared midgut …

Expression Of Genes Encoding Ca2+ Exporting Proteins In Freshwater Crayfish Procambarus Clarkii During Cold Exposure, Christopher Gillen

Christopher M. Gillen

1. This study examined expression of two primary transmembrane Ca2+ export proteins (plasma membrane Ca2+ ATPase, (PMCA); Na+/Ca2+ exchanger, sodium/calcium exchanger (NCX)) in epithelial (antennal gland, kidney) and non-epithelial (axial abdominal muscle) tissues of the freshwater crayfish Procambarus clarkii following exposure (28 days) to 4 °C (compared with control 23 °C). 2. Crayfish tissues responded to cold exposure within 5–7 days by upregulating the mRNA (determined through real-time polymerase chain reaction (PCR)) for both PMCA and NCX. After 28 days, levels were 4–5× higher in antennal gland and 2–3× higher in muscle. In situ hybridization localized the mRNA increase to …

The Cation-Chloride Cotransporter, Masbsc, Is Widely Expressed In Manduca Sexta Tissues., Haruhiko Itagaki, Christopher Gillen, Kathy Gillen

Christopher M. Gillen

Cation-chloride cotransporters, including the Na-K-Cl cotransporter, play an important role in epithelial ion transport in insects. We have determined the tissue distribution of Manduca sexta bumetanide sensitive cotransporter (masBSC), a putative Na-K-Cl cotransporter that was originally cloned from M. sexta Malpighian tubules. We developed a polyclonal antibody (M6) against a C-terminal fragment of masBSC. masBSC protein was detected by M6 at an apparent molecular mass of approximately 220kDa in M. sexta foregut, midgut, hindgut, Malpighian tubule, salivary gland, fat body, trachea, and nerve cord. Higher expression was observed in the foregut than in other tissues. M6 stained the apical membrane …

Procambarus Clarkii Sarcoplasmic Calcium-Binding Protein Variant A (Scp1) Mrna, Complete Cds, M. J. Northcutt, Christopher M. Gillen

Christopher M. Gillen

Procambarus Clarkii Sarcoplasmic Calcium-Binding Protein Variant B (Scp1) Mrna, Complete Cds, Christopher M. Gillen, M. J. Northcutt

Christopher M. Gillen

Procambarus Clarkii Sarcoplasmic Calcium-Binding Protein Variant C (Scp1) Mrna, Complete Cds, Christopher M. Gillen, M. J. Northcutt

Christopher M. Gillen

Manduca Sexta Cation-Chloride Cotransporter Gene, Partial Cds, Christopher M. Gillen

Christopher M. Gillen