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Full-Text Articles in Biology
Cd47 Blockade Enhances Macrophage Efferocytosis Via A Process Requiring Low-Density Lipoprotein Receptor-Related Protein 1, Richard Anthony Maldonado
Cd47 Blockade Enhances Macrophage Efferocytosis Via A Process Requiring Low-Density Lipoprotein Receptor-Related Protein 1, Richard Anthony Maldonado
CUP Undergraduate Research
Vulnerable atherosclerotic plaques are characterized by large necrotic cores caused by impaired clearance of apoptotic cells (efferocytosis) in the artery wall. Targeted antibody blockade of CD47 (CD47), an anti-efferocytosis protein, enhances efferocytosis and reduces plaque area in mouse models of atherosclerosis. LDL receptor-related protein-1 (LRP1) is a pro-efferocytosis receptor whose deletion from macrophages accelerates atherogenesis. We recently found that CD47 requires macrophage LRP1 to limit atherosclerosis. Thus, we hypothesize that the enhanced efferocytosis caused by CD47 also requires macrophage LRP1. We conducted in vitro studies using wildtype (WT) and LRP1-/- macrophages as efferocytes and either Jurkat lymphocyte cells or WT …
Reliability Of Chronically Recorded Visually Evoked Potentials In Awake Mouse Visual Cortex: Effect Of Restraint Habituation, Mckenna D. Stocker
Reliability Of Chronically Recorded Visually Evoked Potentials In Awake Mouse Visual Cortex: Effect Of Restraint Habituation, Mckenna D. Stocker
CUP Undergraduate Research
Visual function in mice can be quantified using electrophysiological methods. This can be done using chronically implanted electrodes that record visually evoked potentials (VEPs) from a population of neurons in the primary visual cortex (V1) in order to estimate visual acuity. The inherently noisy environment of the brain presents a challenge, as the VEP signal is very small. Our goal is to optimize VEP recording procedures to produce the highest signal-to-noise ratio possible by investigating the role of restraint habituation. The approach we designed included three experimental groups: one in which the animals received regular stimulus exposure and no habituation, …
The Development Of A Low-Cost Air Quality Sensor Array For Mobile Platforms, Justin Taylor
The Development Of A Low-Cost Air Quality Sensor Array For Mobile Platforms, Justin Taylor
CUP Undergraduate Research
The traditional method of measuring air quality uses large immobile sensors. These sensors can cost hundreds of thousands of dollars. If small, low-cost sensors are proven reliable then they can be attached to a drone for air quality management. The K30 Carbon Dioxide sensor from CO2Meter.com was used for testing. The BME280 sensor from Adafruit was used to measure humidity and temperature. A Raspberry Pi was used as an electronic controller and the programming was written in Python. After testing was completed, the K30 Carbon Dioxide sensor was found inadequate for atmospheric testing. The BME280 was determined effective for air …
Measuring Visual Acuity In Awake Mice Using Visually Evoked Potentials (Veps), Emanuel Drutu
Measuring Visual Acuity In Awake Mice Using Visually Evoked Potentials (Veps), Emanuel Drutu
CUP Undergraduate Research
We investigated a means to measure visual acuity in awake mice using visually evoked potentials (VEPs). Using counter-phasing sinusoidal gratings as stimuli, we compared the effectiveness of vertical and horizontal orientations in generating VEPs. Using stereotaxic implanted electrodes in the animal’s primary visual cortex, the evoked VEPs were recorded and analyzed. At the lowest spatial frequency, vertical stimuli evoked the largest VEP amplitude. However, at higher spatial frequencies, a reversal occurs where horizontal gratings evoked larger VEPs. These data suggest vertical and horizontal stimuli have different effects on generating VEPs although further investigation is required to determine which stimulus is …
Independent Association Of Sudden Cardiac Death And The Tpeak-Tend Interval On Vectorcardiogram, Ryan Richard Gonzales
Independent Association Of Sudden Cardiac Death And The Tpeak-Tend Interval On Vectorcardiogram, Ryan Richard Gonzales
CUP Undergraduate Research
The causes of Sudden Cardiac Death (SCD) are still uncertain in the field of electro-cardiology. Cardiac repolarization may be the instance in which fatal arrhythmias lead to SCD development. Cardiac disease has not been well treated, as it is the leading cause of death in industrialized nations. Correlation of the T wave length to Sudden Cardiac Death (SCD) has been demonstrated in recent studies, most notably in a meta-analysis published in 2017, (Tse, Gong, Wong, Georgopoulos, Letsas, Vassiliou, … Liu, 2017). However, this project is attempting to show independent association of the T wave and SCD. Independent association demonstrates some …
The Use Of Molecular Dynamics Simulations To Predict Thymine Dimer Formation In Histone Bound Dna, Emily Ann Brune Heinsen
The Use Of Molecular Dynamics Simulations To Predict Thymine Dimer Formation In Histone Bound Dna, Emily Ann Brune Heinsen
CUP Undergraduate Research
Thymine dimers are the major lesions in DNA that are caused by environmental conditions that can lead to apoptosis or can become carcinogenic (Schreier, et al., 2007). These dimers form when the pi electrons of one thymine in a neighboring thymine pair is excited by a photon from UV light, and occurs on the picosecond time scale (Schreier, et al., 2007). Due to the ultrafast nature of this reaction, the thymines must be in proper conformation prior to excitation in order for the reaction to take place. The proper conformation for the reaction has been found to occur when the …
Uv Irradiation Of Thymine Molecules And Gas Chromatography – Mass Spectrometry, Kelsey Elaine Mauk
Uv Irradiation Of Thymine Molecules And Gas Chromatography – Mass Spectrometry, Kelsey Elaine Mauk
CUP Undergraduate Research
Cyclobutane pyrimidine dimers (CPDs) are products of a photochemical reaction caused by ultraviolet (UV) irradiation of adjacent pyrimidine molecules. Thymine dimers, the most common form of CPDs in the body, are of particular interest because they are the cause of the majority of UV-induced mutations in mammals, often resulting in the development of skin cancers. Because of the photochemical properties of the reactions of thymine dimers, and the relation of these dimers to cancer pathogenesis, the formation and analysis of thymine dimers in a laboratory setting could be a useful educational tool for pre-medicine students learning about organic and photochemistry. …
Effects Of Ph20 Hyaluronidase Inhibition In Vivo Following Cerebral White Matter Injury, Rachel E. Siltman
Effects Of Ph20 Hyaluronidase Inhibition In Vivo Following Cerebral White Matter Injury, Rachel E. Siltman
CUP Undergraduate Research
Human infants born prematurely between 23-32 weeks have a predisposition toward sustaining white matter injury (WMI). In survivors of preterm birth, hypoxic-ischemic (H-I) damage to the cerebral white matter is the leading cause of a spectrum of neurological and motor deficits and in severe cases, cerebral palsy (CP). H-I triggers extracellular matrix (ECM) breakdown leading to production of inhibitory ECM components. H-I also induces vigorous proliferation of oligodendrocyte progenitor cells (OPCs) leading to accumulation of the pre-oligodendrocytes (preOLs). However, these preOLs fail to differentiate and generate myelin producing oligodendrocytes (OLs). Mechanistically, ECM breakdown blocks repair and regeneration of myelin following …
Invertebrate Neurophysiology: Testing The Efficacy Of Natural Insecticides, Anastasiya S. Ananenka, Wayne Tschetter
Invertebrate Neurophysiology: Testing The Efficacy Of Natural Insecticides, Anastasiya S. Ananenka, Wayne Tschetter
CUP Undergraduate Research
Insecticides are widely used in agriculture. While there are several classes of synthetic insecticides that are highly effective against insects, there is data suggesting that these compounds are also toxic to non-target species. However, compounds like caffeine have been shown to act as natural insecticides. Here we investigate the effects of a wellknown class of insecticides, pyrethroids, as well as natural compounds such as caffeine. Since insecticides work at the level of the nervous system we propose to measure neural activity in crickets (Acheta Domestica) after exposure to synthetic and naturally occurring insecticides. Here we show our approach and preliminary …
Uv Irradiation Of Thymine Molecules And Gas Chromatography-Mass Spectrometry, Kelsey Mauk, Andrew Johnson
Uv Irradiation Of Thymine Molecules And Gas Chromatography-Mass Spectrometry, Kelsey Mauk, Andrew Johnson
CUP Undergraduate Research
When irradiated with UV light, adjacent pyrimidine bases undergo a photochemical reaction to form cyclobutane pyrimidine dimers (CPD)3. Although this damage is often repaired, CPDs can cause issues with DNA replication and transcription, and are believed to be one of the major sources of carcinogenic mutations created through DNA replication2. Although CPDs can be formed between any two pyrimidine bases, the most common dimers formed are between two thymine molecules. Previous studies have shown success in the creation of thymine dimers by irradiating frozen thymine solution with UV light4, however techniques of analysis vary. Gas chromatography-mass spectrometry (GCMS) is an …
The Use Of Molecular Dynamics Simulations To Predict Thymine Dimer Formation In Histone Bound Dna, Emily Heinsen
The Use Of Molecular Dynamics Simulations To Predict Thymine Dimer Formation In Histone Bound Dna, Emily Heinsen
CUP Undergraduate Research
No abstract provided.
Uv Irradiation Of Thymine Molecules And Gas Chromatography – Mass Spectrometry, Kelsey Mauk
Uv Irradiation Of Thymine Molecules And Gas Chromatography – Mass Spectrometry, Kelsey Mauk
CUP Undergraduate Research
No abstract provided.
Extracellular Matrix Breakdown Blocks Repair And Regeneration Following Preterm Brain Injury, Rachel Siltman, Stephen Black, Taasin Srivastava
Extracellular Matrix Breakdown Blocks Repair And Regeneration Following Preterm Brain Injury, Rachel Siltman, Stephen Black, Taasin Srivastava
CUP Undergraduate Research
No abstract provided.
2013 Ii Cu Suri (Common Poster), Vanessa Selimovic, Loriann Reese, Ruthie Nelson, Emma Sleeman, Hibery Ho, Maria Polozova
2013 Ii Cu Suri (Common Poster), Vanessa Selimovic, Loriann Reese, Ruthie Nelson, Emma Sleeman, Hibery Ho, Maria Polozova
CUP Undergraduate Research
A common poster providing information for several research projects: aerosol particles, deoxycytidine kinase, squaraine rotaxanes, glycol nucleic acied, and Kombucha.
Bacterial Analysis Of Refrigerated Human Milk Following Infant Feeding, Rachel Renee Brusseau
Bacterial Analysis Of Refrigerated Human Milk Following Infant Feeding, Rachel Renee Brusseau
CUP Undergraduate Research
The number of infants who are breastfed is on the rise, as is the number of women in the workforce. Many women who choose breastfeeding after returning to work, express milk during the day and store this milk for a future feeding. When infants do not finish a bottle of expressed breastmilk, doctors recommend unfinished portions be thrown away. This study examined bacterial levels in expressed, partially consumed breastmilk that was stored for 48 hours at 4-6° C. A portion of unconsumed milk was examined as a control. Samples were taken every 12 hours for bacterial analysis. Tests were performed …