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Articles 181 - 210 of 397

Full-Text Articles in Biology

Progesterone And Prostaglandin F2Α Induce Species-Typical Female Preferences For Male Sexual Displays In Cope's Gray Treefrog (Hyla Chrysoscelis), J. L. Ward, E. K. Love, Alexander T. Baugh, N. M. Gordon, J. C. Tanner, M. A. Bee Dec 2015

Progesterone And Prostaglandin F2Α Induce Species-Typical Female Preferences For Male Sexual Displays In Cope's Gray Treefrog (Hyla Chrysoscelis), J. L. Ward, E. K. Love, Alexander T. Baugh, N. M. Gordon, J. C. Tanner, M. A. Bee

Biology Faculty Works

Endocrine systems play critical roles in facilitating sexual behavior in seasonally breeding vertebrates. Much of the research exploring this topic has focused on the endocrine correlates of signaling behavior in males and sexual proceptivity in females. What is less understood is how hormones promote the expression of the often complex and highly selective set of stimulus–response behaviors that are observed in naturally breeding animals. In female frogs, phonotaxis is a robust and sensitive bioassay of mate choice and is exhibited by gravid females during the breeding season. In stark contrast, females exhibit low phonotactic responsiveness outside the breeding season, but …


Review Of "Race Unmasked: Biology And Race In The Twentieth Century" By M. Yudell, John B. Jenkins Dec 2015

Review Of "Race Unmasked: Biology And Race In The Twentieth Century" By M. Yudell, John B. Jenkins

Biology Faculty Works

No abstract provided.


Anthropologists Are Talking – About The Anthropocene, D. Haraway, N. Ishikawa, Scott F. Gilbert, K. Olwig, A. L. Tsing, N. Bubandt Nov 2015

Anthropologists Are Talking – About The Anthropocene, D. Haraway, N. Ishikawa, Scott F. Gilbert, K. Olwig, A. L. Tsing, N. Bubandt

Biology Faculty Works

No abstract provided.


No Effect Of Host Species On Phenoloxidase Activity In A Mycophagous Beetle, Vincent A. Formica, Amanda Kar-Men Chan , '16 Oct 2015

No Effect Of Host Species On Phenoloxidase Activity In A Mycophagous Beetle, Vincent A. Formica, Amanda Kar-Men Chan , '16

Biology Faculty Works

Ecological immunology is an interdisciplinary field that helps elucidate interactions between the environment and immune response. The host species individuals experience have profound effects on immune response in many species of insects. However, this conclusion comes from studies of herbivorous insects even though species of mycophagous insects also inhabit many different host species. The goal of this study was to determine if fungal host species as well as individual, sex, body size, and host patch predict one aspect of immune function, phenoloxidase activity (PO). We sampled a metapopulation of Bolitotherus cornutus, a mycophagous beetle in southwestern Virginia. B. cornutus live …


Parameter Estimation For Gene Regulatory Networks From Microarray Data: Cold Shock Response In Saccharomyces Cerevisiae, Kam D. Dahlquist, Ben G. Fitzpatrick, Erika T. Camacho, Stephanie D. Entzminger, Nathan C. Wanner Oct 2015

Parameter Estimation For Gene Regulatory Networks From Microarray Data: Cold Shock Response In Saccharomyces Cerevisiae, Kam D. Dahlquist, Ben G. Fitzpatrick, Erika T. Camacho, Stephanie D. Entzminger, Nathan C. Wanner

Biology Faculty Works

We investigated the dynamics of a gene regulatory network controlling the cold shock response in budding yeast, Saccharomyces cerevisiae. The medium-scale network, derived from published genome-wide location data, consists of 21 transcription factors that regulate one another through 31 directed edges. The expression levels of the individual transcription factors were modeled using mass balance ordinary differential equations with a sigmoidal production function. Each equation includes a production rate, a degradation rate, weights that denote the magnitude and type of influence of the connected transcription factors (activation or repression), and a threshold of expression. The inverse problem of determining model …


An Inquiry-Infused Introductory Biology Laboratory That Integrates Mendel's Pea Phenotypes With Molecular Mechanisms, Philip Kudish, E. Schlag, Nicholas J. Kaplinsky May 2015

An Inquiry-Infused Introductory Biology Laboratory That Integrates Mendel's Pea Phenotypes With Molecular Mechanisms, Philip Kudish, E. Schlag, Nicholas J. Kaplinsky

Biology Faculty Works

We developed a multi-week laboratory in which college-level introductory biology students investigate Mendel's stem length phenotype in peas. Students collect, analyze and interpret convergent evidence from molecular and physiological techniques. In weeks 1 and 2, students treat control and experimental plants with Gibberellic Acid (GA) to determine whether uncharacterized short mutant lines are GA responsive. These data allow students to place the mutation in the GA signal transduction pathway. During weeks 2 and 3, plants are genotyped for Mendel's "le" mutation using a derived cleaved polymorphic sequences (dCAPS) PCR assay. This laboratory allows students to make a direct connection between …


The Fossil Record Of Phenotypic Integration And Modularity: A Deep-Time Perspective On Developmental And Evolutionary Dynamics, Anjali Goswami, Wendy Binder, Julie Meachen, F. Robin O’Keefe Apr 2015

The Fossil Record Of Phenotypic Integration And Modularity: A Deep-Time Perspective On Developmental And Evolutionary Dynamics, Anjali Goswami, Wendy Binder, Julie Meachen, F. Robin O’Keefe

Biology Faculty Works

Variation is the raw material for natural selection, but the factors shaping variation are still poorly understood. Genetic and developmental interactions can direct variation, but there has been little synthesis of these effects with the extrinsic factors that can shape biodiversity over large scales. The study of phenotypic integration and modularity has the capacity to unify these aspects of evolutionary study by estimating genetic and developmental interactions through the quantitative analysis of morphology, allowing for combined assessment of intrinsic and extrinsic effects. Data from the fossil record in particular are central to our understanding of phenotypic integration and modularity because …


Dna-Based Authentication Of Botanicals And Plant-Derived Dietary Supplements: Where Have We Been And Where Are We Going?, Denise F. Coutinho Moraes, David W. Still, Michelle R. Lum, Ann M. Hirsch Apr 2015

Dna-Based Authentication Of Botanicals And Plant-Derived Dietary Supplements: Where Have We Been And Where Are We Going?, Denise F. Coutinho Moraes, David W. Still, Michelle R. Lum, Ann M. Hirsch

Biology Faculty Works

Herbal medicines and botanicals have long been used as sole or additional medical aids worldwide. Currently, billions of dollars are spent on botanicals and related products, but minimal regulation exists regarding their purity, integrity, and efficacy. Cases of adulteration and contamination have led to severe illness and even death in some cases. Identifying the plant material in botanicals and phytomedicines using organoleptic means or through microscopic observation of plant parts is not trivial, and plants are often misidentified. Recently, DNA-based methods have been applied to these products because DNA is not changed by growth conditions unlike the chemical constituents of …


Textures And Traction: How Tube-Dwelling Polychaetes Get A Leg Up, Rachel Merz Mar 2015

Textures And Traction: How Tube-Dwelling Polychaetes Get A Leg Up, Rachel Merz

Biology Faculty Works

By controlling the traction between its body and the tube wall, a tube-dwelling polychaete can move efficiently from one end of its tube to the other, brace its body during normal functions (e.g., ventilation and feeding), and anchor within its tube avoiding removal by predators. To examine the potential physical interaction between worms and the tubes they live in, scanning electron microscopy was used to reveal and quantify the morphology of worm bodies and the tubes they produce for species representing 13 families of tube-dwelling polychaetes. In the tubes of most species there were macroscopic or nearly macroscopic (~10 μm–1 …


Trehalose Is A Chemical Attractant In The Establishment Of Coral Symbiosis, M. Hagedorn, V. Carter, N. Zuchowicz, M. Phillips, C. Penfield, B. Shamenek, Elizabeth A. Vallen, F. W. Kleinhans, K. Peterson, M. White, P. H. Yancey Jan 2015

Trehalose Is A Chemical Attractant In The Establishment Of Coral Symbiosis, M. Hagedorn, V. Carter, N. Zuchowicz, M. Phillips, C. Penfield, B. Shamenek, Elizabeth A. Vallen, F. W. Kleinhans, K. Peterson, M. White, P. H. Yancey

Biology Faculty Works

Coral reefs have evolved with a crucial symbiosis between photosynthetic dinoflagellates (genus Symbiodinium) and their cnidarian hosts (Scleractinians). Most coral larvae take up Symbiodinium from their environment; however, the earliest steps in this process have been elusive. Here we demonstrate that the disaccharide trehalose may be an important signal from the symbiont to potential larval hosts. Symbiodinium freshly isolated from Fungia scutaria corals constantly released trehalose (but not sucrose, maltose or glucose) into seawater, and released glycerol only in the presence of coral tissue. Spawning Fungia adults increased symbiont number in their immediate area by excreting pellets of Symbiodinium, and …


Thermal Variation, Thermal Extremes And The Physiological Performance Of Individuals, W. Wesley Dowd, Felicia A. King, Mark W. Denny Jan 2015

Thermal Variation, Thermal Extremes And The Physiological Performance Of Individuals, W. Wesley Dowd, Felicia A. King, Mark W. Denny

Biology Faculty Works

In this review we consider how small-scale temporal and spatial variation in body temperature, and biochemical/physiological variation among individuals, affect the prediction of organisms’ performance in nature. For ‘normal’ body temperatures – benign temperatures near the species’ mean – thermal biology traditionally uses performance curves to describe how physiological capabilities vary with temperature. However, these curves, which are typically measured under static laboratory conditions, can yield incomplete or inaccurate predictions of how organisms respond to natural patterns of temperature variation. For example, scale transition theory predicts that, in a variable environment, peak average performance is lower and occurs at a …


A Reliable Method For The Selection And Confirmation Of Transconjugants Of Plant Growth-Promoting Bacteria Especially Plant-Associated Burkholderia Spp., Michelle Lum Jan 2015

A Reliable Method For The Selection And Confirmation Of Transconjugants Of Plant Growth-Promoting Bacteria Especially Plant-Associated Burkholderia Spp., Michelle Lum

Biology Faculty Works

Selectable markers, e.g., antibiotic resistance, for conjugation experiments are not always effective for slow-growing plant growth promoting bacteria such as Burkholderia. We used PCAT medium containing Congo Red for selecting Burkholderia transconjugants. This method allows for the reliable selection of transconjugants of these novel plant growth-promoting bacteria.


Proteomic Analysis Of Cardiac Response To Thermal Acclimation In The Eurythermal Goby Fish Gillichthys Mirabilis, W. Wesley Dowd, Nishad Jayasundara, Lars Tomanek, George N. Somero Jan 2015

Proteomic Analysis Of Cardiac Response To Thermal Acclimation In The Eurythermal Goby Fish Gillichthys Mirabilis, W. Wesley Dowd, Nishad Jayasundara, Lars Tomanek, George N. Somero

Biology Faculty Works

Cardiac function is thought to play a central role in determining thermal optima and tolerance limits in teleost fishes. Investigating proteomic responses to temperature in cardiac tissues may provide insights into mechanisms supporting the thermal plasticity of cardiac function. Here, we utilized a global proteomic analysis to investigate changes in cardiac protein abundance in response to temperature acclimation (transfer from 13°C to 9, 19 and 26°C) in a eurythermal goby, Gillichthys mirabilis. Proteomic data revealed 122 differentially expressed proteins across acclimation groups, 37 of which were identified using tandem mass-spectrometry. These 37 proteins are involved in energy metabolism, mitochondrial regulation, …


Mining The Phytomicrobiome To Understand How Bacterial Coinoculations Enhance Plant Growth, Nancy Fujishige Jan 2015

Mining The Phytomicrobiome To Understand How Bacterial Coinoculations Enhance Plant Growth, Nancy Fujishige

Biology Faculty Works

In previous work, we showed that coinoculating Rhizobium leguminosarum bv. viciae 128C53 and Bacillus simplex 30N-5 onto Pisum sativum L. roots resulted in better nodulation and increased plant growth. We now expand this research to include another alpha-rhizobial species as well as a beta-rhizobium, Burkholderia tuberum STM678. We first determined whether the rhizobia were compatible with B. simplex 30N-5 by cross-streaking experiments, and then Medicago truncatula and Melilotus alba were coinoculated with B. simplex 30N-5 and Sinorhizobium (Ensifer) meliloti to determine the effects on plant growth. Similarly, B. simplex 30N-5 and Bu. tuberum STM678 were coinoculated onto Macroptilium atropurpureum. The …


Mia40 Protein Serves As An Electron Sink In The Mia40-Erv1 Import Pathway, Sonya E. Neal, Deepa V. Dabir, Heather L. Tienson, Darryl M. Horn, Kathrin Glaeser, Rachel R. Ogozalek Loo, Antoni Barrientos, Carla M. Koehler Jan 2015

Mia40 Protein Serves As An Electron Sink In The Mia40-Erv1 Import Pathway, Sonya E. Neal, Deepa V. Dabir, Heather L. Tienson, Darryl M. Horn, Kathrin Glaeser, Rachel R. Ogozalek Loo, Antoni Barrientos, Carla M. Koehler

Biology Faculty Works

A redox-regulated import pathway consisting of Mia40 and Erv1 mediates the import of cysteine-rich proteins into the mitochondrial intermembrane space. Mia40 is the oxidoreductase that inserts two disulfide bonds into the substrate simultaneously. However, Mia40 has one redox-active cysteine pair, resulting in ambiguity about how Mia40 accepts numerous electrons during substrate oxidation. In this study, we have addressed the oxidation of Tim13 in vitro and in organello. Reductants such as glutathione and ascorbate inhibited both the oxidation of the substrate Tim13 in vitro and the import of Tim13 and Cmc1 into isolated mitochondria. In addition, a ternary complex consisting …


Patterns Of Testosterone In Three Nearctic-Neotropical Migratory Songbirds During Spring Passage, Kristen M. Covino Jan 2015

Patterns Of Testosterone In Three Nearctic-Neotropical Migratory Songbirds During Spring Passage, Kristen M. Covino

Biology Faculty Works

Preparation for breeding may overlap extensively with vernal migration in long-distance migratory songbirds. Testosterone plays a central role in mediating this transition into breeding condition by facilitating changes to physiology and behavior. While changes in testosterone levels are well studied in captive migrants, these changes are less well known in free-living birds. We examined testosterone levels in free-living Nearctic-Neotropical migrants of three species during their vernal migration. Testosterone levels increased during the migratory period in males of all three species but significantly so in only two. Testosterone levels in females remained the same throughout their migration. Our results support the …


Fall Migratory Patterns Of The Blackpoll Warbler At A Continental Scale, Kristen M. Covino Jan 2015

Fall Migratory Patterns Of The Blackpoll Warbler At A Continental Scale, Kristen M. Covino

Biology Faculty Works

Blackpoll Warblers (Setophaga striata) breed across Canada and the northern United States and fly across the Atlantic Ocean to South America in fall. Studies of fall migration in this species have centered on the extensive overwater flights but have ignored other aspects of this migration, including potential geographic variation. We used Blackpoll Warbler banding records from 3 coastal (Atlantic seaboard) sites and 9 inland sites to study the fall migration. The sites covered a wide range of the species' geographic distribution during breeding and fall migration in North America. The data spanned the years 1967-2013, though the particular years sampled …


Review Of "The Pku Paradox: A Short History Of A Genetic Disease" By D. B. Paul And J. P. Brosco, John B. Jenkins Dec 2014

Review Of "The Pku Paradox: A Short History Of A Genetic Disease" By D. B. Paul And J. P. Brosco, John B. Jenkins

Biology Faculty Works

No abstract provided.


The Origin And Loss Of Periodic Patterning In The Turtle Shell, J. E. Moustakas-Verho, R. Zimm, J. A. Cebra-Thomas, N. K. Lempiainen, A. Kallonen, K. L. Mitchell, K. Hamalainen, I. Salazar-Ciudad, J. Jernvall, Scott F. Gilbert Aug 2014

The Origin And Loss Of Periodic Patterning In The Turtle Shell, J. E. Moustakas-Verho, R. Zimm, J. A. Cebra-Thomas, N. K. Lempiainen, A. Kallonen, K. L. Mitchell, K. Hamalainen, I. Salazar-Ciudad, J. Jernvall, Scott F. Gilbert

Biology Faculty Works

The origin of the turtle shell over 200 million years ago greatly modified the amniote body plan, and the morphological plasticity of the shell has promoted the adaptive radiation of turtles. The shell, comprising a dorsal carapace and a ventral plastron, is a layered structure formed by basal endochondral axial skeletal elements (ribs, vertebrae) and plates of bone, which are overlain by keratinous ectodermal scutes. Studies of turtle development have mostly focused on the bones of the shell; however, the genetic regulation of the epidermal scutes has not been investigated. Here, we show that scutes develop from an array of …


A Holobiont Birth Narrative: The Epigenetic Transmission Of The Human Microbiome, Scott F. Gilbert Aug 2014

A Holobiont Birth Narrative: The Epigenetic Transmission Of The Human Microbiome, Scott F. Gilbert

Biology Faculty Works

This essay plans to explore, expand, and re-tell the human birth narrative. Usually, human birth narratives focus on the origins of a new individual, focusing on the mother and fetus. This essay discusses birth as the origin of a new community. For not only is the eukaryotic body being reproduced, but so also are the bodies of its symbiotic microbes and so is the set of relationships between these organic components. Several parts of the new narrative are surprising: (1) bacterial symbionts might cause some of the characteristics of pregnancy and prepare a symbiotic community for transfer; (2) the first …


Insect Visitation Of Peduncular And Petiolar Extrafloral Nectar Glands On Castor Bean (Ricinus Communis L.) Plants In Southern California, Victor D. Carmona-Galindo, Taylor Waters, David Chirikian Jun 2014

Insect Visitation Of Peduncular And Petiolar Extrafloral Nectar Glands On Castor Bean (Ricinus Communis L.) Plants In Southern California, Victor D. Carmona-Galindo, Taylor Waters, David Chirikian

Biology Faculty Works

Castor bean (Ricinus communis L.) is a myrmecophytic plant species with specialized extrafloral nectar (EFN) glands that serve to attract predatory insects, which in return defend plant-tissues against herbivores. The EFN glands on castor bean plants are located along the leaf petioles as well as on the peduncles of its imperfect (unisexual) flowers. This field-project evaluates the richness, diversity, and species assemblage of insects visiting EFN glands located on (female and male) flower peduncles and leaf petioles on castor bean plants growing in a Southern California coastal landscape. We detected that EFN glands on female-flower peduncles were visited by …


Exploration Of Climate Influences On The Abundance Of Galls On Red Willow (Salix /Aevigata) Across Two Riparian Communities In Southern California, Victor D. Carmona-Galindo, Rachael Sears, Tauras P. Vilgalys, Emily Hand, Sara Morledge-Hampton May 2014

Exploration Of Climate Influences On The Abundance Of Galls On Red Willow (Salix /Aevigata) Across Two Riparian Communities In Southern California, Victor D. Carmona-Galindo, Rachael Sears, Tauras P. Vilgalys, Emily Hand, Sara Morledge-Hampton

Biology Faculty Works

In Southern California, the red willow (Sa/ix laevigata Bebb) hosts a variety of gall-inducing parasitic insects. However, little is known about the ecology of these parasites, particularly the characterization of their microclimate preferences. This study explores the relationship between microclimate and gall frequencies in S. laevigata in the Ballona Wetlands and Temescal Canyon, and gall count correlated with biotic and abiotic factors such as soil pH, soil moisture and willow density. Significantly more galls per leaf were found at Temescal Canyon than Ballona Wetlands. Although the number of galls per leaf correlated negatively with soil pH, soil moisture content and …


Symbiosis As The Way Of Eukaryotic Life: The Dependent Co-Origination Of The Body, Scott F. Gilbert Apr 2014

Symbiosis As The Way Of Eukaryotic Life: The Dependent Co-Origination Of The Body, Scott F. Gilbert

Biology Faculty Works

Molecular analyses of symbiotic relationships are challenging our biological definitions of individuality and supplanting them with a new notion of normal part whole relationships. This new notion is that of a 'holobiont', a consortium of organisms that becomes a functionally integrated 'whole'. This holobiont includes the zoological organism (the 'animal') as well as its persistent microbial symbionts. This new individuality is seen on anatomical and physiological levels, where a diversity of symbionts form a new 'organ system' within the zoological organism and become integrated into its metabolism and development. Moreover, as in normal development, there are reciprocal interactions between the …


Review Of "Once We All Had Gills: Growing Up Evolutionist In An Evolving World" By R. A. Raff, Scott F. Gilbert Mar 2014

Review Of "Once We All Had Gills: Growing Up Evolutionist In An Evolving World" By R. A. Raff, Scott F. Gilbert

Biology Faculty Works

No abstract provided.


Differential Resource Allocation Of Black Mustard Plants (Brassica Nigra L.) With Proximity To Black Walnut Trees (Jug/Ans Californica L.) In A Southern California Riparian Ecosystem, Victor D. Carmona-Galindo, Matt Cronrod, Kellyanne Gold Feb 2014

Differential Resource Allocation Of Black Mustard Plants (Brassica Nigra L.) With Proximity To Black Walnut Trees (Jug/Ans Californica L.) In A Southern California Riparian Ecosystem, Victor D. Carmona-Galindo, Matt Cronrod, Kellyanne Gold

Biology Faculty Works

The invasive forb Brassica nigra (black mustard) and the native tree Jug/ans californica (black walnut) are allelopathic species that suppress the growth of neighboring plants. This study evaluates how allocation to root, reproductive, and photosynthetic biomass in B. nigra was affected with proximity to J. califomica. We hypothesized that a joint suppressive effect would lead to lower root biomass allocation in B. nigra near J. californica due to reduced interspecific competition. Our results indicate that B. nigra plants growing near J. califomica had significantly lower root : total biomass ratios, and provide insights into how to effectively control this invasive …


Formalizing Theories Of Development: A Fugue On The Orderliness Of Change, Scott F. Gilbert, J. Bard Jan 2014

Formalizing Theories Of Development: A Fugue On The Orderliness Of Change, Scott F. Gilbert, J. Bard

Biology Faculty Works

This chapter looks at developmental biology as performance. Each animal inherits score (the DNA), mechanisms for interpreting of the score, and mechanisms for improvisation should the score be deficient. Developmental causation is found to be both upwards from the genome, downward from the environment, and laterally between cells. Developmental plasticity, organicism, phenotypic heterogeneity, symbiotic co-development, and cytoplasmic localization are each examples of causation from the environment downward. Stereocomplementary relationships are the key components of most developmental interactions. These interactions can be placed into a formal language of graph theory. Morphogenesis can be depicted in the general structure, where nouns cover …


Cranial Morphometrics Of The Dire Wolf, Canis Dirus, At Rancho La Brea: Temporal Variability And Its Links To Nutrient Stress And Climate, F. Robin O'Keefe, Wendy J. Binder, Stephen R. Frost, Rudyard W. Sadlier, Blaire Van Valkenburgh Jan 2014

Cranial Morphometrics Of The Dire Wolf, Canis Dirus, At Rancho La Brea: Temporal Variability And Its Links To Nutrient Stress And Climate, F. Robin O'Keefe, Wendy J. Binder, Stephen R. Frost, Rudyard W. Sadlier, Blaire Van Valkenburgh

Biology Faculty Works

The tar pits of Rancho La Brea are a unique window onto the biology and ecology of the terminal Pleistocene in southern California. In this study we capitalize on recent advances in understanding of La Brea tar pit chronology to perform the first morphometric study of crania of the dire wolf, Canis dirus, over time. We first present new data on tooth fracture and wear from pits dated older than heretofore analyzed, and demonstrate that fracture and wear events, and the increased competition and heightened carcass utilization they are thought to represent, were of varying intensity across the sampled …


Pvr Expression Regulators In Equilibrium Signal Control And Maintenance Of Drosophila Blood Progenitors, Bama C.Haran Mondal, Jiwon Shim, Cory J. Evans, Utpal Banerjee Jan 2014

Pvr Expression Regulators In Equilibrium Signal Control And Maintenance Of Drosophila Blood Progenitors, Bama C.Haran Mondal, Jiwon Shim, Cory J. Evans, Utpal Banerjee

Biology Faculty Works

Blood progenitors within the lymph gland, a larval organ that supports hematopoiesis in Drosophila melanogaster, are maintained by integrating signals emanating from niche-like cells and those from differentiating blood cells. We term the signal from differentiating cells the ‘equilibrium signal’ in order to distinguish it from the ‘niche signal’. Earlier we showed that equilibrium signaling utilizes Pvr (the Drosophila PDGF/VEGF receptor), STAT92E, and adenosine deaminase-related growth factor A (ADGF-A) (Mondal et al., 2011). Little is known about how this signal initiates during hematopoietic development. To identify new genes involved in lymph gland blood progenitor maintenance, particularly those involved in equilibrium …


Drosophila Hematopoiesis: Markers And Methods For Molecular Genetic Analysis, Cory J. Evans, Ting Liu, Utpal Banerjee Jan 2014

Drosophila Hematopoiesis: Markers And Methods For Molecular Genetic Analysis, Cory J. Evans, Ting Liu, Utpal Banerjee

Biology Faculty Works

Analyses of the Drosophila hematopoietic system are becoming more and more prevalent as developmental and functional parallels with vertebrate blood cells become more evident. Investigative work on the fly blood system has, out of necessity, led to the identification of new molecular markers for blood cell types and lineages and to the refinement of useful molecular genetic tools and analytical methods. This review briefly describes the Drosophila hematopoietic system at different developmental stages, summarizes the major useful cell markers and tools for each stage, and provides basic protocols for practical analysis of circulating blood cells and of the lymph gland, …


Stage-Dependent Plasticity Of The Anterior Neural Folds To Form Neural Crest, Maxellende Ezin Jan 2014

Stage-Dependent Plasticity Of The Anterior Neural Folds To Form Neural Crest, Maxellende Ezin

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The anterior neural fold (ANF) is the only region of the neural tube that does not produce neural crest cells. Instead, ANF cells contribute to the olfactory and lens placodes, as well as to the forebrain and epidermis. Here, we test the ability of the ANF to form neural crest by performing heterotopic transplantation experiments in the chick embryo. We find that, at the neurula stage (HH stage 7), the chick ANF retains the ability to form migrating neural crest cells when transplanted caudally to rostral hindbrain levels. This ability is gradually lost, such that by HH9, this tissue appears …