Biology Commons^™

Philibaetis Gen. Nov., A New Genus From The Philippines (Ephemeroptera, Baetidae), Thomas Kaltenbach, Jhoana M. Garces, Jean-Luc Gattolliat

Biology Faculty Publications

Investigations of type material and new material from the Philippines (Luzon) revealed that Baetis luzonensis Müller-Liebenau, 1982 and B. realonae Müller-Liebenau, 1982 do not belong to Baetis Leach, 1815. A new genus, Philibaetis gen. nov., is described to accommodate both species and both are re-described based on larvae. The new genus is characterised by having a rectangular labrum with a submarginal row of long, simple setae on the dorsal surface and ventrally on lateral margins long, simple, spine-like setae, on anterolateral margins long, feathered setae and medially long, bifid setae and a partial, submarginal row of lanceolate setae. Both mandibles …

Rapid Survey Of Anuran Species In Baguio-Benguet Area And Isolation Of Their Fungal Symbionts, Arthien Lovell P. Pelingen, Camille Andrea Flores, Axel John Briz, Roland Hipol, Celia Austria

Biology Faculty Publications

Baguio City is one of the fastest-growing centers of urbanization in the Philippines. As part of the Cordillera Mountain Range, it is a biodiversity hotspot that is largely unexplored. This study is a preliminary investigation using visual encounter surveys of anuran species in various localities of the Baguio-Benguet area (BBA). These include Loakan Airport (LA), Camp John Hay (CJH), and Soroptimist Compound (SC) at the Military Cut Off, all in Baguio; Nangalisan, Tuba (NT), Benguet; and Master’s Garden (MG) at Puguis, La Trinidad, Benguet. We documented three Luzon-endemic species of frogs – namely, Kaloula rigida (Family Microhylidae), Sanguirana luzonensis (Family …

Aquatic Coleoptera Of Northern Negros, Philippines, Marc Ryan Sabordo, Carl Michael Buyco, Gwendalyn Henares, Jessica Pacalioga, Hendrik Freitag

Biology Faculty Publications

The water beetle fauna of northern Negros Island has not been comprehensively studied. In this study, aquatic Coleoptera of northern Negros have been compiled from various collections. Specimens were sampled through manual sampling and light trap. Twenty-six (26) species were recorded including 21 which are Philippine endemics. Nine genera and three known species are recorded for the first time from Negros.. Notes on distribution, ecology and identification are provided. Possible new species, future potential discoveries as well as current significance in terms of conservation are discussed.

Hydraena (S.Str.) Dinarica, New Species (Coleoptera: Hydraenidae) Along With Further Records Of Hydraena Spp. From Durmitor National Park, Montenegro And Comments On The Dna Barcoding Problem With The Genus, Hendrik Freitag, Rick De Vries, Marta Paterno, Simone Maestri, Massimo Delledonne, Cameron G. Thompson, Helena Lamed, Rebekah Lambert, Michael F. Fox, Mariela C. Gonzalez, Emmanuel D. Delocado, Marc R. Sabordo, Clister V. Pangantihon, Iva Njunjić

Biology Faculty Publications

Background Long-palped Water Beetles were collected during a taxon expedition in Montenegro which involved citizen scientists, students and taxonomists. The material was collected from springs, brooks, fens and the Tara River, at altitudes between 600 m and 1450 m above sea level, using fine-meshed hand-nets and by manual checking of submerged substrates. The morphological species delimitation was supplemented and congruent with mtDNA sequences mainly obtained in the field using the newly-developed MinION-based ONTrack pipeline. New information The new species Hydraena dinarica Freitag & de Vries, sp. n. from Durmitor Mt. is described, illustrated and compared in detail to closely-related congeners …

Discovering Genes Involved In The Synthesis Of Secondary Metabolites From The Seeds Of Moringa Oleifera Through Transcriptome Analysis, Vivian A. Panes, Ma. Divina Kristi A. Discar

Biology Faculty Publications

Moringa oleifera is a widely used crop that produces seeds with a plethora of benefits encompassing health and nutrition. Secondary metabolite compounds were determined in the seeds of Moringa oleifera that possess nutritional and pharmacological benefits. Although various phytochemical researchers reported the presence of secondary metabolites in M. oleifera seeds, there is a lack of research on the genes encoding for enzymes that catalyze the synthesis of secondary metabolites in the seeds of M. oleifera. In the present study, RNA sequencing was used to analyze the transcriptome of the mature seed embryos of M. oleifera. Biological pathway analysis …

Aquatic Coleoptera Of Northern Negros, Philippines, Marc Ryan Sabordo, Carl Michael Buyco, Gwendalyn Henares, Jessica Pacalioga, Hendrik Freitag

Biology Faculty Publications

The water beetle fauna of northern Negros Island has not been comprehensively studied. In this study, aquatic Coleoptera of northern Negros have been compiled from various collections. Specimens were sampled through manual sampling and light trap. Twenty-six (26) species were recorded including 21 which are Philippine endemics. Nine genera and three known species are recorded for the first time from Negros. Notes on distribution, ecology and identification are provided. Possible new species, future potential discoveries as well as current significance in terms of conservation are discussed.

Seed Priming With Phytohormones: An Effective Approach For The Mitigation Of Abiotic Stress, Mohammad Saidur Rhaman, Shahin Imran, Farjana Rauf, Mousumi Khatun, Carol C. Baskin, Yoshiyuki Murata, Mirza Hasanuzzaman

Biology Faculty Publications

Plants are often exposed to abiotic stresses such as drought, salinity, heat, cold, and heavy metals that induce complex responses, which result in reduced growth as well as crop yield. Phytohormones are well known for their regulatory role in plant growth and development, and they serve as important chemical messengers, allowing plants to function during exposure to various stresses. Seed priming is a physiological technique involving seed hydration and drying to improve metabolic processes prior to germination, thereby increasing the percentage and rate of germination and improving seedling growth and crop yield under normal and various biotic and abiotic stresses. …

Larval Anatomy Of Monotypic Painted Ant Nest Frogs Lithodytes Lineatus Reveals Putative Homoplasies With The Leptodactylus Pentadactylus Group (Anura: Leptodactylidae), Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The morphological diversity of anuran larvae made them an important source of information for evolutionary and systematic studies. For the monotypic frog genus Lithodytes, which has an interesting taxonomic history, including its past synonymizing with Adenomera and its placement as a subgenus of Leptodactylus, the information provided from its larvae can help to understand its systematics interrelationships and also provide insights about its evolutionary trajectories. Herein, we provide a detailed description of the larval morphology of Lithodytes lineatus, including novel data of internal morphology (buccopharyngeal cavity and skeleton), and discuss some morphological and evolutionary aspects in relation …

Water Availability For Cannabis In Northern California: Intersections Of Climate, Policy, And Public Discourse, Betsy Morgan, Kaitlyn Spangler, Jacob Stuivenvolt-Allen, Christina N. Morrisett, Mark W. Brunson, Shih-Yu Simon Wang, Nancy Huntly

Biology Faculty Publications

Availability of water for irrigated crops is driven by climate and policy, as moderated by public priorities and opinions. We explore how climate and water policy interact to influence water availability for cannabis (Cannabis sativa), a newly regulated crop in California, as well as how public discourse frames these interactions. Grower access to surface water covaries with precipitation frequency and oscillates consistently in an energetic 11–17 year wet-dry cycle. Assessing contemporary cannabis water policies against historic streamflow data showed that legal surface water access was most reliable for cannabis growers with small water rights ( < 600 m³) and …

Taxonomic Review Of South American Butter Frogs: Phylogeny, Geographic Patterns, And Species Delimitation In The Leptodactylus Latrans Species Group (Anura: Leptodactylidae), Felipe De M. Magalhães, Mariana L. Lyra, Thiago R. De Carvalho, Diego Baldo, Francisco Brusquetti, Pamela Burella, Guarino R. Colli, Marcelo C. Gehara, Rafael O. De Sá, Et Al.

Biology Faculty Publications

The Leptodactylus latrans species group currently comprises eight medium- to large-sized frog species with a convoluted taxonomic history, particularly related to the specific limits of the L. latrans complex, and the species pair Leptodactylus chaquensis–Leptodactylus macrosternum. Their homogeneous external morphology and continental geographic distribution in South America have posed severe limitations to a comprehensive review, such that taxonomic consensus and species limits remain uncertain. This is further worsened by the presence of chromatic polymorphism among coexisting species that can hardly be distinguished by external morphology. Based on a large-scale geographic sampling including multilocus DNA analyses, and acoustic …

A Two-Clone Approach To Study Signaling Interactions Among Neuronal Cells In A Pre-Clinical Alzheimer’S Disease Model, Catherine J. Yeates, Ankita Sarkar, Prajakta Deshpande, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

To understand the progression of Alzheimer's disease, studies often rely on ectopic expression of amyloid-beta 42 (Aβ42) throughout an entire tissue. Uniform ectopic expression of Aβ42 may obscure cell-cell interactions that contribute to the progression of the disease. We developed a two-clone system to study the signaling cross talk between GFP-labeled clones of Aβ42-expressing neurons and wild-type neurons simultaneously generated from the same progenitor cell by a single recombination event. Surprisingly, wild-type clones are reduced in size as compared with Aβ42-producing clones. We found that wild-type cells are eliminated by the induction of cell death. Furthermore, aberrant activation of c-Jun-N-terminal …

Does The Presence Of Multiple Β-Lactamases In Gram-Negative Bacilli Impact The Results Of Antimicrobial Susceptibility Tests And Extended-Spectrum Β-Lactamase And Carbapenemase Confirmation Methods?, Fred C. Tenover, Caitlin M. Dela Cruz, Scott Dewell, Victoria M. Le, Isabella A. Tickler

Biology Faculty Publications

Objectives: Many multidrug-resistant Gram-negative bacilli (MDR-GNB) harbour multiple β-lactamases. The aim of this study was to assess the impact of multiple β-lactamase carriage on the accuracy of susceptibility tests and extended-spectrum β-lactamase (ESBL) and carbapenemase confirmation methods.

Methods: A total of 50 MDR-GNB, of which 29 carried multiple β-lactamases, underwent broth microdilution (BMD) and disk diffusion (DD) testing as well as confirmation tests for ESBLs and carbapenemases. Whole-genome sequencing (WGS) was used for β-lactamase gene identification.

Results: Categorical agreement of BMD and DD testing results ranged from 86.5 to 97.7% for 10 β-lactam agents. BMD and DD algorithms for ESBL …

Draft Genome Of The Common Snapping Turtle, Chelydra Serpentina, A Model For Phenotypic Plasticity In Reptiles, Debojyoti Das, Sunil Kumar Singh, Jacob Bierstedt, Alyssa Erickson, Gina L. J. Galli, Dane A. Crossley Ii, Turk Rhen

Biology Faculty Publications

Turtles are iconic reptiles that inhabit a range of ecosystems from oceans to deserts and climates from the tropics to northern temperate regions. Yet, we have little understanding of the genetic adaptations that allow turtles to survive and reproduce in such diverse environments. Common snapping turtles, Chelydra serpentina, are an ideal model species for studying adaptation to climate because they are widely distributed from tropical to northern temperate zones in North America. They are also easy to maintain and breed in captivity and produce large clutch sizes, which makes them amenable to quantitative genetic and molecular genetic studies of …

Plant Single-Cell Metabolomics—Challenges And Perspectives, Leonardo Perez De Souza, Monica Borghi, Alisdair R. Fernie

Biology Faculty Publications

Omics approaches for investigating biological systems were introduced in the mid-1990s and quickly consolidated to become a fundamental pillar of modern biology. The idea of measuring the whole complement of genes, transcripts, proteins, and metabolites has since become widespread and routinely adopted in the pursuit of an infinity of scientific questions. Incremental improvements over technical aspects such as sampling, sensitivity, cost, and throughput pushed even further the boundaries of what these techniques can achieve. In this context, single-cell genomics and transcriptomics quickly became a well-established tool to answer fundamental questions challenging to assess at a whole tissue level. Following a …

Evaluation Of The Xpert Carba-R Nxg Assay For Detection Of Carbapenemase Genes In A Global Challenge Set Of Pseudomonas Aeruginosa Isolates, Fred C. Tenover, Christian M. Gill, Tomefa E. Asempa, Isabella A. Tickler, Caitlin M. Dela Cruz, David P. Nicolau

Biology Faculty Publications

The growing prevalence and diversity of carbapenemase producers among carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates warrants an expansion of detection capabilities. The purpose of this study was to evaluate the performance of the commercially available Xpert Carba-R (Carba-R) and the research-use-only Xpert Carba-R NxG (Carba-R NxG) in a global collection of P. aeruginosa. The challenge set included 123 P. aeruginosa clinical isolates from 12 countries. Isolates were previously categorized via PCR or whole-genome sequencing. Carbapenemase classes tested include VIM, IMP, NDM, SPM, KPC, and GES. Non-carbapenemase (non-CP)-harboring isolates were also tested (negative control). Isolates were tested using the Carba-R NxG and …

Stream Dissolved Organic Matter In Permafrost Regions Shows Surprising Compositional Similarities But Negative Priming And Nutrient Effects, Ethan Wologo, Sarah Shakil, Scott Zolkos, Sadie Textor, Stephanie Ewing, Jane Klassen, Robert G. M. Spencer, David C. Podgorski, Suzanne E. Tank, Michelle A. Baker, Jonathan A. O'Donnell, Kimberly P. Wickland, Sydney S. W. Foks, Jay P. Zarnetske, Joseph Lee-Cullin, Futing Liu, Yuanhe Yang, Pirkko Kortelainen, Jaana Kolehmainen, Joshua F. Dean, Jorien E. Vonk, Robert M. Holmes, Gilles Pinay, Michaela M. Powell, Jansen Howe, Rebecca J. Frei, Samuel P. Bratsman, Benjamin W. Abbott

Biology Faculty Publications

Permafrost degradation is delivering bioavailable dissolved organic matter (DOM) and inorganic nutrients to surface water networks. While these permafrost subsidies represent a small portion of total fluvial DOM and nutrient fluxes, they could influence food webs and net ecosystem carbon balance via priming or nutrient effects that destabilize background DOM. We investigated how addition of biolabile carbon (acetate) and inorganic nutrients (nitrogen and phosphorus) affected DOM decomposition with 28-day incubations. We incubated late-summer stream water from 23 locations nested in seven northern or high-altitude regions in Asia, Europe, and North America. DOM loss ranged from 3% to 52%, showing a …

Climate-Related Geographical Variation In Performance Traits Across The Invasion Front Of A Widespread Non-Native Insect, Lily M. Thompson, Sean D. Powers, Ashley Appolon, Petra Hafker, Leila Milner, Dylan Parry, Salvatore J. Agosta, Kristine L. Grayson

Biology Faculty Publications

Aim

Invasive species are ideal systems for testing geographical differences in performance traits and measuring evolutionary responses as a species spreads across divergent climates and habitats. The European gypsy moth, Lymantria dispar dispar L. (Lepidoptera: Erebidae), is a generalist forest defoliator introduced to Medford, Massachusetts, USA in 1869. The invasion front extends from Minnesota to North Carolina and the ability of this species to adapt to local climate may contribute to its continuing spread. We evaluated the performance of populations along the climatic gradient of the invasion front to test for a relationship between climate and ecologically important performance traits. …

Increasing Our Ability To Predict Contemporary Evolution, Patrik Nosil, Samuel M. Flaxman, Jeffrey L. Feder, Zachariah Gompert

Biology Faculty Publications

Classic debates concerning the extent to which scientists can predict evolution have gained new urgency as environmental changes force species to adapt or risk extinction. We highlight how our ability to predict evolution can be constrained by data limitations that cause poor understanding of deterministic natural selection. We then emphasize how such data limits can be reduced with feasible empirical effort involving a combination of approaches.

Contributions To The Knowledge Of Water Bugs In Mindoro Island, Philippines, With A Species Checklist Of Nepomorpha And Gerromorpha (Insecta, Hemiptera, Heteroptera), Arthien Lovell Pelingen, Herbert Zettel, Clister V. Pangantihon, Kyra Mari Dominique Aldaba, Earl Kevin Fatallo, Jemillie Madonna De Leon, Hendrik Freitag

Biology Faculty Publications

This survey aims to provide an updated species checklist of aquatic and semi-aquatic bugs in the intra-Philippine biogeographic Region of Mindoro. An assessment survey of water bugs (Hemiptera, Heteroptera) was conducted mostly by manual collection in selected areas of Oriental Mindoro from 2017 to 2018, in which some of the collecting activities were undertaken by graduate students of Ateneo de Manila University. Twenty-nine aquatic and semi-aquatic heteropteran species were documented and some are known island-endemic species or subspecies, including Enithares martini mindoroensis Nieser & Zettel, 1999, Hydrotrephes stereoides mindoroensis Zettel, 2003, Aphelocheirus freitagi Zettel & Pangantihon, 2010, Rhagovelia mindoroensis Zettel, …

Shoot Extracts From Two Low Nodulation Mutants Significantly Reduce Nodule Number In Pea, Christian A. Huynh, Frédérique Guinel

Biology Faculty Publications

E107 and E132 are pea mutants that nodulate poorly. Because they have a shoot-controlled nodulation phenotype, we asked if their mutated genes were implicated in the autoregulation of nodulation (AON), a mechanism which consists of two systemic circuits, the positive CEP/CRA2 and the negative CLE/SUNN, coordinated via NIN and miR2111. We further characterized the mutants’ phenotype by studying nodule distribution and nodulation efficiency. E107 was similar to wild-type (WT) in its nodule distribution, but E132 had an extended nodulation zone with nodules forming distally on its lateral roots. Moreover, we tested whether their shoots produced a compound inhibitory to nodulation. …

Spiny Mice (Acomys) Exhibit Attenuated Hallmarks Of Aging And Rapid Cell Turnover After Uv Exposure In The Skin Epidermis, Wesley Wong, Austin Kim, James R. Monaghan, Ashley W. Seifert, Malcolm Maden, Justin D. Crane

Biology Faculty Publications

The study of long-lived and regenerative animal models has revealed diverse protective responses to stressors such as aging and tissue injury. Spiny mice (Acomys) are a unique mammalian model of skin wound regeneration, but their response to other types of physiological skin damage has not been investigated. In this study, we examine how spiny mouse skin responds to acute UVB damage or chronological aging compared to non-regenerative C57Bl/6 mice (M. musculus). We find that, compared to M. musculus, the skin epidermis in A. cahirinus experiences a similar UVB-induced increase in basal cell …

Comparable Human Reconstitution Following Cesium-137 Versus X-Ray Irradiation Preconditioning In Immunodeficient Nog Mice, Anna Halling Folkmar Andersen, Stine Sofie Frank Nielsen, Rikka Olesen, Jakob Le Fèvre Harslund, Ole S. Søgaard, Lars Østergaard, Paul Denton, Martin Tolstrup

Biology Faculty Publications

Humanized mouse models are used extensively in research involving human pathogens and diseases. However, most of these models require preconditioning. Radio-active sources have been used routinely for this purpose but safety issues have motivated researchers to transition to chemical or X-ray based preconditioning. In this study, we directly compare 350 kV X-ray and Cs-137 low-dose precondition of NOG mice before human stem cell transplantation. Based on flow cytometry data, we found that engraftment of human cells into the mouse bone marrow was similar between radiation sources. Likewise, human engraftment in the peripheral blood was comparable between Cs-137 and three different …

Tadpole Of The Amazonia Frog Edalorhina Perezi (Anura: Leptodactylidae) With Description Of Oral Internal And Chondrocranial Morphology, Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The genus Edalorhina consists of two species of small forest-floor frogs inhabiting the Amazon basin. The tadpole of Edalorhina perezi, the most widely distributed species, was previously described based on a single and early stage (Gosner 25) individual. Herein, we provide a description of the tadpole in Gosner stages 35–36 including internal morphology data (i.e., buccopharyngeal cavity and larval skeleton) based on samples from two populations from Ecuador. Edalorhina shares a generalized morphology with most members of its closely related taxa; however, it is distinguished from the other species by having an almost terminal oral disc. The presence of …

Field Evaluation Of Recombinant Antigen Elisa In Detecting Zoonotic Schistosome Infection Among Water Buffaloes In Endemic Municipalities In The Philippines, Jose Ma. M. Angeles, Yasuyuki Goto, Masahi Kirinoki, Elena A. Villacorte, Kharleezelle J. Moendeg, Pilarita T. Rivera, Yuichi Chigusa, Shin-Ichiro Kawazu

Biology Faculty Publications

In this study, we investigated the use of recombinant antigens thioredoxin peroxidase-1 (rSjTPx-1) and tandem repeat rSj1TR in evaluating the antibody positivity rates of Schistosoma japonicum infection among water buffaloes from four endemic areas in the Philippines, two municipalities with high endemicity (Calatrava, Negros Occidental and Catarman, Northern Samar) and two municipalities nearing elimination with no cases of human schistosomiasis (Talibon and Trinidad, Bohol). These recombinant antigen ELISA assays were compared with other diagnostic tests including SEA-ELISA, FECT, and fecal-based PCR. Results showed that rSj1TR-ELISA has the highest agreement with PCR in all study areas. Furthermore, significant positivity rates among …

Herb-Layer Dynamics In An Old-Growth Forest: Vegetation–Environment Relationships And Response To Invasion-Related Perturbations, Taylor M. Buskey, Margaret E. Maloney, Julia I. Chapman, Ryan W. Mcewan

Biology Faculty Publications

Temperate forests of eastern North America are subject to multiple invasions from non-native species that have the potential to drive long-term dynamics in biodiversity. Garlic mustard (Alliaria petiolata (M. Bieb.) Cavara and Grande) is an invasive plant in many deciduous forests, and management efforts often focus on removing this species to initiate native species restoration. Agrilus planipennis Fairmaire (emerald ash borer; Coleoptera: Buprestidae) is a non-native insect pest that has caused substantial loss of ash trees (Fraxinus spp. L.) in North America. Our goal was to understand how the herbaceous layer in an old-growth forest responded to the removal of …

Mechanistic Insights Into Strigolactone Biosynthesis, Signaling And Regulation During Plant Growth And Development, Kaiser Iqbal Wani, Andleeb Zehra, Sadaf Choudhary, M Naeem, M. Masroor A. Khan, Christian Danve Castroverde, Tariq Aftab

Biology Faculty Publications

Strigolactones (SLs) constitute a group of carotenoid-derived phytohormones with butenolide moieties. These hormones are involved in various functions, including regulation of secondary growth, shoot branching and hypocotyl elongation, and stimulation of seed germination. SLs also control hyphal branching of arbuscular mycorrhizal (AM) fungi, and mediate responses to both abiotic and biotic cues. Most of these functions stem from the interplay of SLs with other hormones, enabling plants to appropriately respond to changing environmental conditions. This dynamic interplay provides opportunities for phytohormones to modulate and augment one another. In this article, we review our current mechanistic understanding of SL biosynthesis, receptors …

A Tree Frog (Boana Pugnax) Dataset Of Skin Transcriptome For The Identification Of Biomolecules With Potential Antimicrobial Activities, Yamil Liscano Martinez, Claudia Marcela Arenas Gómez, Jeramiah J. Smith, Jean Paul Delgado

Biology Faculty Publications

Increases in the prevalence of multiply resistant microbes have necessitated the search for new molecules with antimicrobial properties. One noteworthy avenue in this search is inspired by the presence of native antimicrobial peptides in the skin of amphibians. Having the second highest diversity of frogs worldwide, Colombian anurans represent an extensive natural reservoir that could be tapped in this search. Among this diversity, species such as Boana pugnax (the Chirique-Flusse Treefrog) are particularly notable, in that they thrive in a diversity of marginal habitats, utilize both aquatic and arboreal habitats, and are members of one of few genera that are …

Comparison Of Thermal And Hydrotime Requirements For Seed Germination Of Seven Stipa Species From Cool And Warm Habitats, Rui Zhang, Kai Luo, Dali Chen, Jerry M. Baskin, Carol C. Baskin, Yanrong Wang, Xiaowen Hu

Biology Faculty Publications

Temperature and water potential are two important environmental factors influencing germination and subsequent seedling establishment. Seed germination requirements vary with species and with the environment in which the seeds are produced. Stipa species dominate large areas of the Eurasian zonal vegetation, but comparisons of germination requirements between Stipa species from different habitats is limited. We investigated the effects of temperature and water potential on seed germination of S. grandis, S. purpurea, and S. penicillata from habitats with low temperatures and relatively abundant rainfall (cool habitats) and S. glareosa, S. breviflora, S. gobiea, and S. bungeana …

Does The Presence Of Multiple Β-Lactamases In Gram-Negative Bacilli Impact The Results Of Antimicrobial Susceptibility Tests And Extended-Spectrum Β-Lactamase And Carbapenemase Confirmation Methods?, Fred C. Tenover, Caitlin M. Dela Cruz, Scott Dewell, Victoria M. Le, Isabella A. Tickler

Biology Faculty Publications

Objectives: Many multidrug-resistant Gram-negative bacilli (MDR-GNB) harbour multiple β-lactamases. The aim of this study was to assess the impact of multiple β-lactamase carriage on the accuracy of susceptibility tests and extended-spectrum β-lactamase (ESBL) and carbapenemase confirmation methods.

Methods: A total of 50 MDR-GNB, of which 29 carried multiple β-lactamases, underwent broth microdilution (BMD) and disk diffusion (DD) testing as well as confirmation tests for ESBLs and carbapenemases. Whole-genome sequencing (WGS) was used for β-lactamase gene identification.

Results: Categorical agreement of BMD and DD testing results ranged from 86.5 to 97.7% for 10 β-lactam agents. BMD and DD algorithms for ESBL …

Taxonomic Revision Of Agraphydrus Régimbart , 1903 V. Philippine Species And Their First Dna Barcodes (Coleoptera: Hydrophilidae: Acidocerinae), Hendrik Freitag, Albrecht Komarek

Biology Faculty Publications

The genus Agraphydrus RÉGIMBART , 1903 (Coleoptera: Hydrophilidae) of the Republic of the Philippines is taxonomically reviewed aided by COI mtDNA barcodes. Nine new species are described, all currently regarded as endemic to the Philippines: A. abrasus sp.n., A. ampullatus sp.n., A. batak sp.n., A. brevilobatus sp.n., A. occultus sp.n., A. palawanensis sp.n., A. pelingeni sp.n., A. tenuipalpis sp.n., and A. zetteli sp.n. Agraphydrus coomani (O RCHYMONT , 1927), widespread in the Oriental and Australasian regions, is recorded from the Philippines for the first time. COI sequences and thorough morphological examination suggest that this and further species represent a group …