Biology Commons^™

Models Of Density‐Dependent Genic Selection And A New Rock‐Paper‐Scissors Social System, Barry Sinervo, Benoit Heulin, Yann Surget-Groba, Jean Clobert, Donald B. Miles, Ammon Corl, Alexis Chaine, Alison Davis

Biology Faculty Publications

We describe new ESS models of density regulation driven by genic selection to explain the cyclical dynamics of a social system that exhibits a rock-paper-scissors (RPS) set of three alternative strategies. We tracked changes in morph frequency and fitness of Lacerta vivipara and found conspicuous RPS cycles. Morphs of Uta and Lacerta exhibited parallel survival-performance trade-offs. Frequency cycles in both species of lizards are driven by genic selection. In Lacerta, frequency of each allele in adult cohorts had significant impacts on juvenile recruitment, similar to mutualistic, altruistic, and antagonistic relations of RPS alleles in Uta. We constructed evolutionarily stable strategy …

The Cytomegalovirus Homolog Of Interleukin-10 Requires Phosphatidylinositol 3-Kinase Activity For Inhibition Of Cytokine Synthesis In Monocytes, Juliet V. Spencer

Biology Faculty Publications

Human cytomegalovirus (CMV) has evolved numerous strategies for evading host immune defenses, including piracy of cellular cytokines. A viral homolog of interleukin-10, designated cmvIL-10, binds to the cellular IL-10 receptor and effects potent immune suppression. The signaling pathways employed by cmvIL-10 were investigated, and the classic IL-10R/JAK1/Stat3 pathway was found to be activated in monocytes. However, inhibition of JAK1 had little effect on cmvIL-10-mediated suppression of tumor necrosis factor alpha (TNF-α) production. Inhibition of the phosphatidylinositol 3-kinase/Akt pathway had a more significant impact on TNF-α levels but did not completely relieve the immune suppression, demonstrating that cmvIL-10 stimulates multiple signaling …

Recent Advances In Basic Neurosciences And Brain Disease: From Synapses To Behavior, Guo-Qiang Bi, Vadim Bolshakov, Guojun Bu, Catherine M. Cahill, Zhou-Feng Chen, Graham L. Collingridge, Robin L. Cooper, Jens R. Coorssen, Alaa El-Husseini, Vasco Galhardo, Wen-Biao Gan, Jianguo Gu, Kazuhide Inoue, John Isaac, Koichi Iwata, Zhengping Jia, Bong-Kiun Kaang, Mikito Kawamata, Satoshi Kida, Eric Klann, Tatsuro Kohno, Min Li, Xiao-Jiang Li, John F. Macdonald, Karim Nader, Peter V. Nguyen, Uhtaek Oh, Ke Ren, John C. Roder, Michael W. Salter, Weihong Song, Shuzo Sugita, Shao-Jun Tang, Yuanxiang Tao, Yu Tian Wang, Newton Woo, Melanie A. Woodin, Zhen Yan, Megumu Yoshimura, Ming Xu, Zao C. Xu, Xia Zhang, Mei Zhen, Min Zhuo

Biology Faculty Publications

Understanding basic neuronal mechanisms hold the hope for future treatment of brain disease. The 1st international conference on synapse, memory, drug addiction and pain was held in beautiful downtown Toronto, Canada on August 21-23, 2006. Unlike other traditional conferences, this new meeting focused on three major aims: (1) to promote new and cutting edge research in neuroscience; (2) to encourage international information exchange and scientific collaborations; and (3) to provide a platform for active scientists to discuss new findings. Up to 64 investigators presented their recent discoveries, from basic synaptic mechanisms to genes related to human brain disease. This meeting …

Karyotypes Of Eight Species Of Leptodactylus (Anura, Leptodactylidae) With A Description Of A New Karyotype For The Genus, Renata Cecília Amaro-Ghilardi, Gabriel Skuk, Rafael O. De Sá, Miguel Trefaut Rodrigues, Yatiyo Yonenaga-Yassuda

Biology Faculty Publications

Karyotypes of eight species of Leptodactylus (Anura, Leptodactylidae) with a description of a new karyotype for the genus. Eight species of the Neotropical genus Leptodactylus were karyologically studied: seven of them (L. gracilis, L. mystacinus, L. petersii, L. pustulatus, L. macrosternum, L. ocellatus, L. labyrinthicus) presented 2n=22 and L. silvanimbus showed a distinctive karyotype with 2n=24. Nucleolar organizer regions (Ag-NORs) were detected in two different pairs of chromosomes: pair 4 at the proximal region of the long arm of one individual of L. mystacinus from São Paulo state and of L. petersii; and …

Genetic Resolution Of The Enigmatic Lesser Antillean Distribution Of The Frog Leptodactylus Validus (Anura, Leptodactylidae), Keneth Yanek, W. R. Heyer, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus validus has an unusual distribution, inhabiting Trinidad, Tobago, and the Lesser Antilles, but not the mainland of South America. This distribution is inconsistent with other distribution patterns observed for these islands. Although slight variation in adult morphology has been observed among the different island populations of L. validus, call data suggest the presence of a single species. Calls of L. pallidirostris from Venezuela and Brazil suggested that this taxon might be conspecific with L. validus. Sequence data from the 12S and 16S mt rDNA genes indicate that L. validus represents a single species throughout its distribution and is conspecific …

Investigation Of De Novo Cholesterol Synthetic Capacity In The Gonads Of Goldfish (Carassius Auratus) Exposed To The Phytosterol Beta-Sitosterol, Rainie L. Sharpe, Melissa Drolet, Deborah L. Maclatchy

Biology Faculty Publications

Total and intra-mitochondrial gonadal cholesterol concentrations are decreased in fish exposed to the phytoestrogen beta-sitosterol (beta-sit). The present study examined the potential for beta-sit to disrupt de novo cholesterol synthesis in the gonads of goldfish exposed to 200 microgram/g beta-sit and 10 microgram/g 17beta-estradiol (E2; estrogenic control) by intra-peritoneal Silastic® implants for 21 days. The de novo cholesterol synthetic capacity was estimated by incubating gonadal tissue with 14C-acetate for a period of 18 hours, followed by chloroform/methanol lipid extraction and thin layer chromatography (TLC) lipid separation. Lipid classes were confirmed using infrared spectroscopy. Plasma testosterone (T) and total cholesterol concentration …

Identification Of Micrornas And Other Small Rnas From The Adult Newt Eye, Evgeny Makarev, Jason R. Spence, Katia Del Rio-Tsonis, Panagiotis A. Tsonis

Biology Faculty Publications

MicroRNAs (miRNAs) are capable of controlling gene expression by targeting complimentary sequences in many mRNAs. Thus, a small number of miRNAs are capable of regulating expression of many different genes. miRNAs have been found in all animals from Drosophila to human and they are highly conserved. This work was undertaken in order to identify such RNAs in the newt eye.

The Fat Cadherin Acts Through The Hippo Tumor-Suppressor Pathway To Regulate Tissue Size, Maria Willecke, Fisun Hamaratoglu, Madhuri Kango-Singh, Ryan Udan, Chiao-Lin Chen, Chunyao Tao, Xinwei Zhang, Georg Halder

Biology Faculty Publications

Background: The Hippo tumor-suppressor pathway has emerged as a key signaling pathway that controls tissue size in Drosophila. Merlin, the Drosophila homolog of the human Neurofibromatosis type-2 (NF2) tumor-suppressor gene, and the related protein Expanded are the most upstream components of the Hippo pathway identified so far. However, components acting upstream of Expanded and Merlin, such as transmembrane receptors, have not yet been identified.

Results: Here, we report that the protocadherin Fat acts as an upstream component in the Hippo pathway. Fat is a known tumor-suppressor gene in Drosophila, and fat mutants have severely overgrown imaginal discs. We …

Lobe And Serrate Are Required For Cell Survival During Early Eye Development In Drosophila, Amit Singh, Xiao Shi, Kwang-Wook Choi

Biology Faculty Publications

Organogenesis involves an initial surge of cell proliferation, leading to differentiation. This is followed by cell death in order to remove extra cells. During early development, there is little or no cell death. However, there is a lack of information concerning the genes required for survival during the early cell-proliferation phase. Here, we show that Lobe (L) and the Notch (N) ligand Serrate (Ser), which are both involved in ventral eye growth, are required for cell survival in the early eye disc. We observed that the loss-of-ventral-eye phenotype in L or Ser mutants is due to …

Rana Ocellata Linnaeus, 1758 (Currently Leptodactylus Ocellatus; Amphibia, Anura): Proposed Conservation Of Usage Of The Specific Name By The Designation Of A Neotype, W. Ronald Heyer, Ulisses Caramaschi, Rafael O. De Sá

Biology Faculty Publications

The purpose of this application, under Article 75.6 of the Code, is to conserve the usage of the specific name of Rana ocellata Linnaeus, 1758 for a species of leptodactylid frog from South America by the designation of a neotype. Prevailing usage of the name is threatened by the identity of the type specimen which is a different species than that which is currently known as Leptodactylus ocellatus. It is proposed that all name-bearing types be set aside and a neotype designated in accord with prevailing usage.

Expression Of The Primary Carbohydrate Component Of The Bordetella Bronchiseptica Biofilm Matrix Is Dependent On Growth Phase But Independent Of Bvg Regulation, Yasuhiko Irie, Andrew Preston, Ming H. Yuk

Biology Faculty Publications

We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix was xylose, and linkage analysis indicated a majority of it to be in a 4-linked polymeric form. The production of xylose was independent of Bvg regulation but instead was dependent on bacterial growth phase. In addition, N-acetyl-glucosamine in the matrix was found to be important for the initial development of the biofilm. These …

The Influence Of Low Humidity, Pseudacteon Flies, And Competition By Solenopsis Xyloni On Solenopsis Invicta, Ricardo A. Ramirez, D C. Thompson, M D. Remmenga

Biology Faculty Publications

Red imported fire ant, Solenopsis invicta Buren, is a serious pest in the southeastern United States. It has caused economic losses in agricultural products, stings can cause anaphylactic shock, and it has reduced biodiversity. S. invicta has displaced native ant species throughout its range, presumably by competitive exclusion. In 1998, S. invicta populations were confirmed in southern New Mexico, resulting in a quarantine of Doña Ana County. Although large populations of the native southern fire ant, S. xyloniMcCook, are common in the Mesilla Valley, only two small populations of S. invicta have been found since the initial quarantine. It is …

Age And Population Structure Of Joshua Trees (Yucca Brevifolia) In The Northwestern Mojave Desert, Kimberly D. Gilliland, Nancy J. Huntly, J. E. Anderson

Biology Faculty Publications

Many desert perennials are long-lived, but there are few data on ages or population structures of desert plants. We used 2 methods to estimate ages of a population of Yucca brevifolia (Joshua tree) in southwestern Utah from a 14-year census of plant sizes. Plant height at the 1st census ranged from 0.08 m to 6.0 m, and trees grew in height at a mean rate of 3.75 cm ⋅ yr^–1. Plants also increased slowly in basal diameter (0.142 cm ⋅ yr^–1) and branch length (0.024 m ⋅ yr^–1), but basal diameter varied greatly from …

Queen Weights Of Polygyne Solenopsis Invicta And S. Xyloni Suggest Reduced Competitive Ability, Ricardo A. Ramirez, D C. Thompson

Biology Faculty Publications

Solenopsis invicta Buren populations were confirmed in southern New Mexico, resulting in a quarantine of Doña Ana County in 1998; however, Solenopsis xyloni McCook remains the dominant fire ant. This study compared the queen weights and egg production of polygyne colonies of both species. There was no difference in live weights of the major worker caste; however, queens were very different in weight. In this study, S. invicta queens weighed almost twice as much as S. xyloni (S. invicta = 11.32 ± 0.25mg vs. S. xyloni = 6.40 ± 0.30mg) and produced over 50% as many eggs as S. xyloni. …

The Distress Calls Of Leptodactylus Chaquensis Cei, 1950 And Leptodactylus Elenae Heyer, 1978 (Anura: Leptodactylidae), José M. Padial, Rafael O. De Sá, Ignacio De La Riva

Biology Faculty Publications

We describe the distress calls of Leptodactylus chaquensis and Leptodactylus elenae. They each consist of a single, long, tonal sound with complex harmonic structure and complex frequency modulation. The distress calls of both species are different from their respective advertisement calls. Moreover, there are also clear interspecific differences. Harmonic frequencies in L. elenae are higher, there are fewer emphasized harmonics and the call is shorter and repeated at a higher rate (sometimes paired) than the call of L. chaquensis. Call duration, call rate, and position of the dominant harmonic change with the strength of the stimulus that the …

Phylogenetic Analyses Of Mtdna Sequences Reveal Three Cryptic Lineages In The Widespread Neotropical Frog Leptodactylus Fuscus (Schneider, 1799) (Anura, Leptodactylidae), Arley Camargo, Rafael O. De Sá, W. Ronald Heyer

Biology Faculty Publications

Leptodactylus fuscus is a neotropical frog ranging from Panamá to Argentina, to the east of the Andes mountains, and also inhabiting Margarita, Trinidad, and the Tobago islands. We performed phylogenetic analyses of 12S rRNA, 16S rRNA, tRNA-Leu, and ND1 mitochondrial (mt) DNA sequences from specimens collected across the geographic distribution of L. fuscus to examine two alternative hypotheses: (i) L. fuscus is a single, widely distributed species, or (ii) L. fuscus is a species complex. We tested statistically for geographic association and partitioning of genetic variation among mtDNA clades. The mtDNA data supported the hypothesis of several cryptic species within …

The Biology Of Canadian Weeds. 133. Cuscuta Campestris Yuncker, C. Gronovii Willd. Ex Schult., C. Umbrosa Beyr. Ex Hook., C. Epithymum (L.) L. And C. Epilinum Weihe, Mihai Costea, François J. Tardif

Biology Faculty Publications

Cuscuta spp. (dodders) are rootless, holoparasitic herbs with filiform stems attached to the host by numerous haustoria. In Canada, Cuscuta gronovii is the most common native species of the genus followed by Cuscuta campestris and C. umbrosa. Cuscuta epithymum and C. epilinum, both introduced species in Canada, occur occasionally. Infestation by Cuscuta spp. can result in serious yield losses and dodders are listed as noxious weeds in British Columbia, Ontario and Québec, and as restricted weeds in Alberta. These plants have evolved special adaptations to ensure their success: germination occurs late in the season when potential hosts are …

The Offspring/Clutch Size Tradeoff In Mammals, E. L. Charnov, S.K. Morgan Ernest

Biology Faculty Publications

The Smith‐Fretwell model for optimal offspring size assumes the existence of an inverse proportional relationship (i.e., trade‐off) between the number of offspring and the amount of resources invested in an individual offspring; virtually all of the many models derived from theirs make the same trade‐off assumption. Over the last 30 years it has become apparent that the predicted proportionality is often not observed when evaluated across species. We develop a general allometric approach to correct for size‐related differences in the resources available for reproduction. Using data on mammals, we demonstrate that the predicted inverse proportional relationship between number of offspring …

A Comparison Of The Species-Time Relationship Across Ecosystems And Taxonomic Groups, Ethan P. White, P. B. Adler, W. K. Lauenroth, R. A. Gill, D. Greenberg, D. M. Kaufman, A. Rassweiler, J. A. Rusak, M. D. Smith, J. R. Steinbeck, R. B. Waide, J. Yao

Biology Faculty Publications

The species-time relationship (STR) describes how the species richness of a community increases with the time span over which the community is observed. This pattern has numerous implications for both theory and conservation in much the same way as the speciesarea relationship (SAR). However, the STR has received much less attention and to date only a handful of papers have been published on the pattern. Here we gather together 984 community time-series, representing 15 study areas and 9 taxonomic groups, and evaluate their STRs in order to assess the generality of the STR, its consistency across ecosystems and taxonomic groups, …

Leptodactylus Fragilis, Mirriiam Muedeking Heyer, W. Ronald Heyer, Rafael O. De Sá

Biology Faculty Publications

Leptodactylus fragilis (Brocchi) White-lipped thin-toed frogs are characteristically defined according to their habitation and age. There is a diagnosis of different species group within Leptodactylus. It is furthermore described from different perspectives and discoveries such as its eggs and karyotype. The distribution of the frog is considered to be prominent occurring form Southernmost Teaxs throughout Mexico and Middle America expanding to Venezuela. The fossil fuel records and literature on Leptodactylus are slighly viewed before studying its nomenclatural history and rarity.

The Amphibian Tree Of Life, Darrel R. Frost, Taran Grant, Julián Faivovich, Raoul H. Bain, Alexander Haas, Célio F.B. Haddad, Rafael O. De Sá

Biology Faculty Publications

The evidentiary basis of the currently accepted classification of living amphibians is discussed and shown not to warrant the degree of authority conferred on it by use and tradition. A new taxonomy of living amphibians is proposed to correct the deficiencies of the old one. This new taxonomy is based on the largest phylogenetic analysis of living Amphibia so far accomplished. We combined the comparative anatomical character evidence of Haas (2003) with DNA sequences from the mitochondrial transcription unit H1 (12S and 16S ribosomal RNA and tRNAValine genes, ø 2,400 bp of mitochondrial sequences) and the nuclear genes histone H3, …

Leptodactylus Fragilis (Brocchi): White-Lipped Thin-Toed Frog, Miriam M. Heyer, W. Ronald Heyer, Rafael O. De Sá

Biology Faculty Publications

Adult Leptodactylus fragilis are small. The head is longer than wide, and the hind limbs are moderately long (Table 1; Heyer and Thompson 2000 provided definitions of adult size and leg length categories for Leptodactylus). Male vocal sacs are laterally expanded and range from lightly gray speckled to darkly pigmented. Male snout is spatulate; female snout is rounded. Male forearms are not hypertrophied and males lack asperities on the thumbs and chest. The dorsum is spotted or blotched with dark markings, which are often chevron-shaped and sometimes confluent. The supratympanic fold is dark brown. A pair of dorsolateral folds …

Identification Of A Family Of Bsp-A Like Surface Proteins Of Entamoeba Histolytica With Novel Leucine Rich Repeats., Paul H. Davis, Zhi Zhang, Minghe Chen, Xiaochun Zhang, Subhra Chakraborty, Samuel L. Stanley Jr.

Biology Faculty Publications

Leucine rich repeats serve as recognition motifs for surface proteins from bacteria and eukaryotes. The BspA protein from Bacteroides forsythus mediates bacterial binding to fibronectin and contains leucine rich repeats of the Treponema pallidum (TpLRRP) family. Here we show that the protozoan parasite Entamoeba histolytica contains multiple BspA-like proteins, including a family of surface proteins which possess a new form of a leucine rich repeat that differs from the standard Treponema pallidum- like leucine rich repeat (TpLRRP) by possessing two conserved cysteine residues.

Eye Development At The Houston "Fly Meeting", Amit Singh

Biology Faculty Publications

Meeting report: The 47th Annual Drosophila Research Conference or "Fly Meeting" took place at Houston, Texas, USA from March 29th- April 2nd, 2006, under the aegis of the Genetics Society of America. The Fly Meeting provides an excellent opportunity for fly researchers to present their work and to get a snapshot of recent developments and upcoming trends in their research field. The fruit fly, Drosophila melanogaster, is a very versatile model to study growth, patterning, neural development, evolution, systemetics and various other facets of biomedical science. The topics presented in the meeting covered a very broad spectrum of fly research. …

Gene Expression And Discovery During Lens Regeneration In Mouse: Regulation Of Epithelial To Mesenchymal Transition And Lens Differentiation, Mario Medvedovic, Craig R. Tomlinson, Mindy Kay Call, Matthew Grogg, Panagiotis A. Tsonis

Biology Faculty Publications

Purpose: It has been shown that after extracapsular lens removal by anterior capsulotomy in the mouse, the lens can be regenerated. However, as the capsular bag is filled with fibers, epithelial to mesenchymal transition (EMT), an event which is common after cataract surgery as well, takes place during early stages. This study, using a unique mouse model, was undertaken to identify novel regulators and networks in order to more clearly understand secondary cataracts at the molecular level.

Intra-Guild Compensation Regulates Species Richness In Desert Rodents: Reply, J. R. Goheen, Ethan P. White, S.K. Morgan Ernest, J. H. Brown

Biology Faculty Publications

Null models have had a long and contentious history in community ecology (Connor and Simberloff 1979, Harvey et al. 1983, Gotelli and Graves 1996, Graves and Rahbek 2005). Much of this debate has arisen because multiple strategies for null models can be used to address a particular question of interest, and the present exchange is no exception. To assess constancy in species richness through time in a desert rodent community, Nichols et al. (2006) have proposed a Markov Chain model as an alternative to the random-walk model we developed (Goheen et al. 2005). While both null models simulate stochastic local …

Intra-Guild Compensation Regulatesspecies Richness In Desert Rodents: Reply, J. R. Goheen, Ethan P. White, S.K. Morgan Ernest, J. H. Brown

Biology Faculty Publications

No abstract provided.

A Switch From Polyandry To Serial Monogamy: Results From A Three Year Tagging Study Of Horseshoe Crabs In Long Island Sound, Jennifer Mattei Ph.D., Paul Goodell, Christine Depierro, Meghann Burke

Biology Faculty Publications

Part of an ongoing population study of the North American Horseshoe Crab, Limulus polyphemus, in Long Island Sound was completed during the 2003 – 2005 spawning seasons at Milford Point, in Milford, CT. Horseshoe crabs range more widely throughout Long Island Sound than expected and exhibit weak site fidelity. Animals originally tagged in Milford were found as far west as Stamford and as far east as Clinton, CT. during the 3 year study. Out of 522 female horseshoe crabs tagged in 2004 only 4 came back to spawn again at Milford Pt. in 2005. The sex ratios of tagged and …

Sal-Site: Integrating New And Existing Ambystomatid Salamander Research And Informational Resources, Jeramiah J. Smith, Srikrishna Putta, John A. Walker, D. Kevin Kump, Amy K. Samuels, James R. Monaghan, David W. Weisrock, Chuck Staben, S. Randal Voss

Biology Faculty Publications

Salamanders of the genus Ambystoma are a unique model organism system because they enable natural history and biomedical research in the laboratory or field. We developed Sal-Site to integrate new and existing ambystomatid salamander research resources in support of this model system. Sal-Site hosts six important resources: 1) Salamander Genome Project: an information-based web-site describing progress in genome resource development, 2) Ambystoma EST Database: a database of manually edited and analyzed contigs assembled from ESTs that were collected from A. tigrinum tigrinum and A. mexicanum, 3) Ambystoma Gene Collection: a database containing full-length protein-coding sequences, 4) Ambystoma Map and Marker …

Empirical Analysis Of The Str Profiles Resulting From Conceptual Mixtures, David R. Paoletti, Travis E. Doom, Carissa M. Krane, Michael L. Raymer, Dan E. Krane

Biology Faculty Publications

Samples containing DNA from two or more individuals can be difficult to interpret. Even ascertaining the number of contributors can be challenging and associated uncertainties can have dramatic effects on the interpretation of testing results. Using an FBI genotypes dataset, containing complete genotype information from the 13 Combined DNA Index System (CODIS) loci for 959 individuals, all possible mixtures of three individuals were exhaustively and empirically computed. Allele sharing between pairs of individuals in the original dataset, a randomized dataset and datasets of generated cousins and siblings was evaluated as were the number of loci that were necessary to reliably …