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Articles 511 - 540 of 1156
Full-Text Articles in Biology
Growth And Competitive Effects Of Centaurea Stoebe Populations In Response To Simulated Nitrogen Deposition, Wei-Ming He, Daniel Montesinos, Giles C. Thelan, Ragan M. Callaway
Growth And Competitive Effects Of Centaurea Stoebe Populations In Response To Simulated Nitrogen Deposition, Wei-Ming He, Daniel Montesinos, Giles C. Thelan, Ragan M. Callaway
Biological Sciences Faculty Publications
Increased resource availability can promote invasion by exotic plants, raising concerns over the potential effects of global increases in the deposition of nitrogen (N). It is poorly understood why increased N favors exotics over natives. Fast growth may be a general trait of good invaders and these species may have exceptional abilities to increase growth rates in response to N deposition. Additionally, invaders commonly displace locals, and thus may have inherently greater competitive abilities. The mean growth response of Centaurea stoebe to two N levels was significantly greater than that of North American (NA) species. Growth responses to N did …
Ngf Causes Trka To Specifically Attract Microtubules To Lipid Rafts, Shona Pryor, Gretchen Mccaffrey, Lindsay R. Young, Mark L. Grimes
Ngf Causes Trka To Specifically Attract Microtubules To Lipid Rafts, Shona Pryor, Gretchen Mccaffrey, Lindsay R. Young, Mark L. Grimes
Biological Sciences Faculty Publications
Membrane protein sorting is mediated by interactions between proteins and lipids. One mechanism that contributes to sorting involves patches of lipids, termed lipid rafts, which are different from their surroundings in lipid and protein composition. Although the nerve growth factor (NGF) receptors, TrkA and p75(NTR) collaborate with each other at the plasma membrane to bind NGF, these two receptors are endocytosed separately and activate different cellular responses. We hypothesized that receptor localization in membrane rafts may play a role in endocytic sorting. TrkA and p75(NTR) both reside in detergent-resistant membranes (DRMs), yet they responded differently to a variety of conditions. …
Different Selective Pressures Lead To Different Genomic Outcomes As Newly-Formed Hybrid Yeasts Evolve, Jeff S. Piotrowski, Saisubramanian Nagarajan, Evgueny Kroll, Alison Stanbery, Kami E. Chiotti, Arthur L. Kruckeberg, Barbara Dunn, Gavin Sherlock, Frank Rosenzweig
Different Selective Pressures Lead To Different Genomic Outcomes As Newly-Formed Hybrid Yeasts Evolve, Jeff S. Piotrowski, Saisubramanian Nagarajan, Evgueny Kroll, Alison Stanbery, Kami E. Chiotti, Arthur L. Kruckeberg, Barbara Dunn, Gavin Sherlock, Frank Rosenzweig
Biological Sciences Faculty Publications
Background: Interspecific hybridization occurs in every eukaryotic kingdom. While hybrid progeny are frequently at a selective disadvantage, in some instances their increased genome size and complexity may result in greater stress resistance than their ancestors, which can be adaptively advantageous at the edges of their ancestors' ranges. While this phenomenon has been repeatedly documented in the field, the response of hybrid populations to long-term selection has not often been explored in the lab. To fill this knowledge gap we crossed the two most distantly related members of the Saccharomyces sensu stricto group, S. cerevisiae and S. uvarum, and established a …
Population-Level Compensation Impedes Biological Control Of An Invasive Forb And Indirect Release Of A Native Grass, Yvette K. Ortega, Dean E. Pearson, Lauren P. Waller, Nancy J. Sturdevant, John L. Maron
Population-Level Compensation Impedes Biological Control Of An Invasive Forb And Indirect Release Of A Native Grass, Yvette K. Ortega, Dean E. Pearson, Lauren P. Waller, Nancy J. Sturdevant, John L. Maron
Biological Sciences Faculty Publications
The intentional introduction of specialist insect herbivores for biological control of exotic weeds provides ideal but understudied systems for evaluating important ecological concepts related to top-down control, plant compensatory responses, indirect effects, and the influence of environmental context on these processes. Centaurea stoebe (spotted knapweed) is a notorious rangeland weed that exhibited regional declines in the early 2000s, attributed to drought by some and to successful biocontrol by others. We initiated an experiment to quantify the effects of the biocontrol agent, Cyphocleonus achates, on Ce. stoebe and its interaction with a dominant native grass competitor, Pseudoroegneria spicata, under …
Cell Signaling. Structural Origins Of Receptor Bias, Stephen R. Sprang, Jackson Chief Elk
Cell Signaling. Structural Origins Of Receptor Bias, Stephen R. Sprang, Jackson Chief Elk
Biological Sciences Faculty Publications
Agonists can elicit pathway-specific conformational changes in a G protein–coupled receptor.
Postdispersal Seed Predation Limits The Abundance Of A Long-Lived Perennial Forb (Lithospermum Ruderale), Mary Carolyn Bricker, John L. Maron
Postdispersal Seed Predation Limits The Abundance Of A Long-Lived Perennial Forb (Lithospermum Ruderale), Mary Carolyn Bricker, John L. Maron
Biological Sciences Faculty Publications
Loss of seeds to consumers is common in plant communities, but the degree to which these losses influence plant abundance or population growth is often unclear. This is particularly the case for postdispersal seed predation by rodents, as most studies of rodent seed predation have focused on the sources of spatiotemporal variation in seed loss but not quantified the population consequences of this loss. In previous work we showed that seed predation by deer mice (Peromyscus maniculatus) substantially reduced seedling recruitment and establishment of Lithospermum ruderale (Boraginaceae), a long-lived perennial forb. To shed light on how rodent seed …
Heterogeneity In Genetic Diversity Among Non-Coding Loci Fails To Fit Neutral Coalescent Models Of Population History, Jeffrey L. Peters, Trina E. Roberts, Kevin Winker, Kevin G. Mccracken
Heterogeneity In Genetic Diversity Among Non-Coding Loci Fails To Fit Neutral Coalescent Models Of Population History, Jeffrey L. Peters, Trina E. Roberts, Kevin Winker, Kevin G. Mccracken
Biological Sciences Faculty Publications
Inferring aspects of the population histories of species using coalescent analyses of non-coding nuclear DNA has grown in popularity. These inferences, such as divergence, gene flow, and changes in population size, assume that genetic data reflect simple population histories and neutral evolutionary processes. However, violating model assumptions can result in a poor fit between empirical data and the models. We sampled 22 nuclear intron sequences from at least 19 different chromosomes (a genomic transect) to test for deviations from selective neutrality in the gadwall (Anas strepera), a Holarctic duck. Nucleotide diversity among these loci varied by nearly two …
Inhibitors Of Membranous Adenylyl Cyclases, Roland Seifert, Gerald H. Lushington, Tung-Chung Mou, Andreas Gille, Stephen R. Sprang
Inhibitors Of Membranous Adenylyl Cyclases, Roland Seifert, Gerald H. Lushington, Tung-Chung Mou, Andreas Gille, Stephen R. Sprang
Biological Sciences Faculty Publications
Membranous adenylyl cyclases (mACs) constitute a family of nine isoforms with different expression patterns. Studies with mAC gene knockout mice provide evidence for the notion that AC isoforms play distinct (patho)physiological roles. Consequently, there is substantial interest in the development of isoform-selective mAC inhibitors. Here, we review the current literature on mAC inhibitors. Structurally diverse inhibitors targeting the catalytic site and allosteric sites (e.g. the diterpene site) have been identified. The catalytic site of mACs accommodates both purine and pyrimidine nucleotides, with a hydrophobic pocket constituting a major affinity-conferring domain for substituents at the 2'- and 3'-O-ribosyl position of nucleotides. …
Distribution, Population Size, And Structure Of Himalayan Grey Goral Naemorhedus Goral Bedfordi (Cetartiodactyla: Bovidae) In Pakistan, Fakhar -I- Abbas, Kathreen E. Ruckstuhl, Afsar Mian, Tanveer Akhtar, Thomas P. Rooney
Distribution, Population Size, And Structure Of Himalayan Grey Goral Naemorhedus Goral Bedfordi (Cetartiodactyla: Bovidae) In Pakistan, Fakhar -I- Abbas, Kathreen E. Ruckstuhl, Afsar Mian, Tanveer Akhtar, Thomas P. Rooney
Biological Sciences Faculty Publications
Himalayan grey goral (Naemorhedus goral bedfordi) is a Red List (Near Threatened) member of Caprinae endemic to Asia, occupying the south-facing slopes of the Himalaya Mountains. The species is listed as Vulnerable on the National Pakistan Red List, but the distribution and abundance of the species within the nation is poorly known. We conducted a national census of Himalayan grey goral in Pakistan during 2002 – 2004, with the objective of documenting the geographic locations of populations, estimating population size, and describing group sizes and sex ratios. We conducted a direct count census in 98 sites distributed over …
Time Of Day Regulates Subcellular Trafficking, Tripartite Synaptic Localization, And Polyadenylation Of The Astrocytic Fabp7 Mrna, Jason R. Gerstner, William M. Vanderheyden, Timothy Lavaute, Cara J. Westmark, Labib Rouhana, Allan I. Pack, Marvin Wickens, Charles F. Landry
Time Of Day Regulates Subcellular Trafficking, Tripartite Synaptic Localization, And Polyadenylation Of The Astrocytic Fabp7 Mrna, Jason R. Gerstner, William M. Vanderheyden, Timothy Lavaute, Cara J. Westmark, Labib Rouhana, Allan I. Pack, Marvin Wickens, Charles F. Landry
Biological Sciences Faculty Publications
The astrocyte brain fatty acid binding protein (Fabp7) has previously been shown to have a coordinated diurnal regulation of mRNA and protein throughout mouse brain, and an age-dependent decline in protein expression within synaptoneurosomal fractions. Mechanisms that control time-of-day changes in expression and trafficking Fabp7 to the perisynaptic process are not known. In this study, we confirmed an enrichment of Fabp7 mRNA and protein in the astrocytic perisynaptic compartment, and observed a diurnal change in the intracellular distribution of Fabp7 mRNA in molecular layers of hippocampus. Northern blotting revealed a coordinated time-of-day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout …
Construction And Transformation Of A Thermotoga-E. Coli Shuttle Vector, Zhaohui Xu, Dongmei Han, Stephen M. Norris
Construction And Transformation Of A Thermotoga-E. Coli Shuttle Vector, Zhaohui Xu, Dongmei Han, Stephen M. Norris
Biological Sciences Faculty Publications
Background: Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems for understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp. Results: Methods for preparing and handling Thermotoga solid cultures under aerobic conditions were optimized. A plating efficiency of similar to 50% was achieved when the bacterial cells were embedded in 0.3% Gelrite. A Thermotoga-E. coli shuttle vector pDH10 was constructed using pRQ7, a cryptic mini-plasmid found …
The Pentatomidae, Or Stink Bugs, Of Kansas With A Key To Species (Hemiptera: Heteroptera), Richard J. Packauskas Ph.D.
The Pentatomidae, Or Stink Bugs, Of Kansas With A Key To Species (Hemiptera: Heteroptera), Richard J. Packauskas Ph.D.
Biological Sciences Faculty Publications
Forty eight species of Pentatomidae are listed as occurring in the state of Kansas, nine of these are new state records. A key to all species known from the state of Kansas is given, along with some notes on new state records.
Effects Of Curcumin On Stem-Like Cells In Human Esophageal Squamous Carcinoma Cell Lines, Michael Eric Geusz, Taghreed N. Almanaa, Roudabeh J. Jamasbi
Effects Of Curcumin On Stem-Like Cells In Human Esophageal Squamous Carcinoma Cell Lines, Michael Eric Geusz, Taghreed N. Almanaa, Roudabeh J. Jamasbi
Biological Sciences Faculty Publications
Background: Many cancers contain cell subpopulations that display characteristics of stem cells. Because these cancer stem cells (CSCs) appear to provide resistance to chemo-radiation therapy, development of therapeutic agents that target CSCs is essential. Curcumin is a phytochemical agent that is currently used in clinical trials to test its effectiveness against cancer. However, the effect of curcumin on CSCs is not well established. The current study evaluated curcumin-induced cell death in six cancer cell lines derived from human esophageal squamous cell carcinomas. Moreover, these cell lines and the ones established from cells that survived curcumin treatments were characterized.Methods: Cell loss …
An At Mutational Bias In The Tiny Gc-Rich Endosymbiont Genome Of Hodgkinia, James T. Van Leuven, John Mccutcheon
An At Mutational Bias In The Tiny Gc-Rich Endosymbiont Genome Of Hodgkinia, James T. Van Leuven, John Mccutcheon
Biological Sciences Faculty Publications
The fractional guanine + cytosine (GC) contents of sequenced bacterial genomes range from 13% to 75%. Despite several decades of research aimed at understanding this wide variation, the forces controlling GC content are not well understood. Recent work has suggested that a universal adenine + thymine (AT) mutational bias exists in all bacteria and that the elevated GC contents found in some bacterial genomes is due to genome-wide selection for increased GC content. These results are generally consistent with the low GC contents observed in most strict endosymbiotic bacterial genomes, where the loss of DNA repair mechanisms combined with the …
A New Forest Fire Paradigm: The Need For High-Severity Fires, Monica L. Bond, Rodney B. Siegel, Richard L. Hutto, Victoria A. Saab, Stephen A. Shunk
A New Forest Fire Paradigm: The Need For High-Severity Fires, Monica L. Bond, Rodney B. Siegel, Richard L. Hutto, Victoria A. Saab, Stephen A. Shunk
Biological Sciences Faculty Publications
Forest fires, particularly those that burn at mixed and high severity (collectively called ‘severe’), have been traditionally perceived as catastrophic events, directing public attention and immense forest management budgets toward fire prevention and suppression. These fires may indeed be catastrophic when measured by losses of human lives and property. However, severe fires in wildland areas are both natural and necessary to maintain the integrity of dynamic, disturbance-adapted forest systems. We propose a change in the current paradigm—which holds that severe forest fires are always harmful—to a new one that embraces their ecological necessity.
The Curious Case Of Arenavirus Entry, And Its Inhibition, Jack H. Nunberg, Joanne York
The Curious Case Of Arenavirus Entry, And Its Inhibition, Jack H. Nunberg, Joanne York
Biological Sciences Faculty Publications
Arenaviruses comprise a diverse family of enveloped negative-strand RNA viruses that are endemic to specific rodent hosts worldwide. Several arenaviruses cause severe hemorrhagic fevers in humans, including Junin and Machupo viruses in South America and Lassa fever virus in western Africa. Arenavirus entry into the host cell is mediated by the envelope glycoprotein complex, GPC. The virion is endocytosed on binding to a cell-surface receptor, and membrane fusion is initiated in response to physiological acidification of the endosome. As with other class I virus fusion proteins, GPC-mediated membrane fusion is promoted through a regulated sequence of conformational changes leading to …
Correlation Of Air Displacement Plethysmography With Alternative Body Fat Measurement Techniques In Men And Women, Brittany L. Reinert, Roberta L. Pohlman, Lynn K. Hartzler
Correlation Of Air Displacement Plethysmography With Alternative Body Fat Measurement Techniques In Men And Women, Brittany L. Reinert, Roberta L. Pohlman, Lynn K. Hartzler
Biological Sciences Faculty Publications
Obesity has reached epidemic proportions with serious health consequences. Techniques used to measure body fat (BF) yield variable BF estimates, and this variability may lead to underestimation or overestimation of BF and subsequent treatment options. The measurements that are most accurate (Dual-Energy X-ray Absorptiometry (DEXA) and Air Displacement Plethysmography (ADP)) are expensive and often unavailable. The purpose of this study is to find the commonly available BF measurement that is the most accurate and practical for individual body types in the general population and compare these measurements to ADP (BOD POD®) as the standard. Field measurements include skinfolds …
Prmt5 And The Role Of Symmetrical Dimethylarginine In Chromatoid Bodies Of Planarian Stem Cells, Labib Rouhana, Ana P. Vieira, Rachel H. Roberts-Galbraith, Phillip A. Newmark
Prmt5 And The Role Of Symmetrical Dimethylarginine In Chromatoid Bodies Of Planarian Stem Cells, Labib Rouhana, Ana P. Vieira, Rachel H. Roberts-Galbraith, Phillip A. Newmark
Biological Sciences Faculty Publications
Planarian flatworms contain a population of adult stem cells (neoblasts) that proliferate and generate cells of all tissues during growth, regeneration and tissue homeostasis. A characteristic feature of neoblasts is the presence of chromatoid bodies, large cytoplasmic ribonucleoprotein (RNP) granules morphologically similar to structures present in the germline of many organisms. This study aims to reveal the function, and identify additional components, of planarian chromatoid bodies. We uncover the presence of symmetrical dimethylarginine (sDMA) on chromatoid body components and identify the ortholog of protein arginine methyltransferase PRMT5 as the enzyme responsible for sDMA modification in these proteins. RNA interference-mediated depletion …
Morphological Variation Of Rusty Crayfish Orconectes Rusticus (Cambaridae) With Gender And Local Scale Spatial Gradients, Luke Etchison, Stephen J. Jacquemin, Michael Allen, Mark Pyron
Morphological Variation Of Rusty Crayfish Orconectes Rusticus (Cambaridae) With Gender And Local Scale Spatial Gradients, Luke Etchison, Stephen J. Jacquemin, Michael Allen, Mark Pyron
Biological Sciences Faculty Publications
We tested for the influence of gender, stream, and urbanization on morphological variation in rusty crayfish (Orconectes rusticus) in an east-central Indiana, US watershed. We used geometric morphometrics to characterize shape and tested for differences among and within sites. Males had shallower rostrum, increased head width and length, decreased abdomen and cephalothorax width and length, and increased telson length compared to females. Morphology of males did not vary with stream or along an urban gradient. The morphology of females varied with stream and along an urban gradient. Female shapes from small creek sites were stouter and less fusiform …
The Sign And Strength Of Plant-Soil Feedback For The Invasive Shrub, Lonicera Maackii, Varies In Different Soils, Kelly Schradin, Don Cipollini
The Sign And Strength Of Plant-Soil Feedback For The Invasive Shrub, Lonicera Maackii, Varies In Different Soils, Kelly Schradin, Don Cipollini
Biological Sciences Faculty Publications
Plants alter soil characteristics causing changes in their subsequent growth resulting in positive or negative feedback on both their own fitness and that of other plants. In a greenhouse study, we investigated whether the sign and strength of feedback changed across two distinct soil types, and whether effects were due to shifts in biotic or abiotic soil traits. Using soils from two different locations, we examined growth of the exotic invasive shrub, Lonicera maackii and the related native shrub, Diervilla lonicera, in unconditioned soils and in soils conditioned by previous growth of L. maackii, D. lonicera, and …
Molecular Characterization And Tissue-Specific Gene Expression Of Dermacentor Variabilis Α-Catenin In Response To Rickettsial Infection, Piyanate Sunyakumthorn, Natthida Petchampai, Michael T. Kearney, Daniel E. Sonenshine, Kevin R. Macaluso
Molecular Characterization And Tissue-Specific Gene Expression Of Dermacentor Variabilis Α-Catenin In Response To Rickettsial Infection, Piyanate Sunyakumthorn, Natthida Petchampai, Michael T. Kearney, Daniel E. Sonenshine, Kevin R. Macaluso
Biological Sciences Faculty Publications
Alpha catenin is a cytoskeleton protein that acts as a regulator of actin rearrangement by forming an E-cadherin adhesion complex. In Dermacentor variabilis, a putative α-catenin (Dvα-catenin) was previously identified as differentially regulated in ovaries of ticks chronically infected with Rickettsia montanensis. To begin characterizing the role(s) of Dvα-catenin during rickettsial infection, the full-length Dvα-catenin cDNA was cloned and analysed. Comparative sequence analysis demonstrates a 3069-bp cDNA with a 2718-bp open reading frame with a sequence similar to Ixodes scapularis α-catenin. A portion of Dvα-catenin is homologous to the vinculin-conserved domain containing a putative …
Fungal Endophytes Directly Increase The Competitive Effects Of An Invasive Forb, Erik T. Aschehoug, Kerry L. Metlen, Ragan M. Callaway, George Newcombe
Fungal Endophytes Directly Increase The Competitive Effects Of An Invasive Forb, Erik T. Aschehoug, Kerry L. Metlen, Ragan M. Callaway, George Newcombe
Biological Sciences Faculty Publications
Competitive outcomes among plants can vary in different abiotic and biotic conditions. Here we tested the effects of two phylotypes of Alternaria endophytes on the growth, competitive effects, and competitive responses of the exotic invasive forb Centaurea stoebe. Centaurea stoebe was a better competitor against North American grass species than grasses from its European home range in the absence of endophytes. However, one endophyte both increased the biomass of C. stoebe and reduced the competitive effect of North American grasses on C. stoebe. The competitive effects of C. stoebe on grass species native to North America were enhanced by both …
The Effect Of Fire On Spatial Separation Between Wolves And Caribou, Hugh S. Robinson, Mark Hebblewhite, Nicholas J. Decesare, Jessie Whittington, Layla Neufeld, Mark Bradley, Marco Musiani
The Effect Of Fire On Spatial Separation Between Wolves And Caribou, Hugh S. Robinson, Mark Hebblewhite, Nicholas J. Decesare, Jessie Whittington, Layla Neufeld, Mark Bradley, Marco Musiani
Biological Sciences Faculty Publications
Fire management is an important conservation tool in Canada’s national parks. Fires can benefit some species, while others may be negatively impacted. We used GPS and VHF collar data for 47 wolves from 12 separate packs and 153 caribou from 5 separate herds, and resource selection analysis to model the effects of fire on these species’ habitat and potential interactions. Resource selection modeling showed that wolves select for burned areas and areas close to burns, presumably due to the presence of primary prey (i.e., elk and moose), while caribou avoid burns. Fire reduced the amount of high quality caribou habitat …
Cyanobacteria Dominance In The Oligohaline Waters Of Back Bay, Virginia, Harold G. Marshall
Cyanobacteria Dominance In The Oligohaline Waters Of Back Bay, Virginia, Harold G. Marshall
Biological Sciences Faculty Publications
Back Bay and its flora have historically been influenced by the interaction of freshwater flow in combination with frequent intrusion of saline water into its basin. These events have resulted in a dynamic environmental setting influencing the abundance and composition of its phytoplankton community. Dominating these oligohaline waters is a diverse representation and high abundance of freshwater filamentous and colonial cyanobacteria. These include the nonheterocystous Planktolyngbya contorta, Planktolyngbya limnetica, and Pseudanabaena limnetica, taxa implicated as bloom producers in Bay waters with N:P molar ratios ranging from 23:1 to 74:1.
Properties Of Lewis Lung Carcinoma Cells Surviving Curcumin Toxicity, Michael Eric Geusz, Dejun Yan
Properties Of Lewis Lung Carcinoma Cells Surviving Curcumin Toxicity, Michael Eric Geusz, Dejun Yan
Biological Sciences Faculty Publications
The anti-inflammatory agent curcumin can selectively eliminate malignant rather than normal cells. The present study examined the effects of curcumin on the Lewis lung carcinoma (LLC) cell line and characterized a subpopulation surviving curcumin treatments. Cell density was measured after curcumin was applied at concentrations between 10 and 60 μM for 30 hours. Because of the high cell loss at 60 μM, this dose was chosen to select for surviving cells that were then used to establish a new cell line. The resulting line had approximately 20% slower growth than the original LLC cell line and based on ELISA contained …
Population Viability Analysis Of The Blue-Throated Macaw (Ara Glaucogularis) Using Individual-Based And Cohort-Based Pva Programs, Juan Luis Bouzat, Rosa I. Strem
Population Viability Analysis Of The Blue-Throated Macaw (Ara Glaucogularis) Using Individual-Based And Cohort-Based Pva Programs, Juan Luis Bouzat, Rosa I. Strem
Biological Sciences Faculty Publications
We developed a demographic model to perform a population viability analysis (PVA) of the Blue-throated Macaw (Ara glaucogularis), a critically endangered species endemic to Bolivia. PVA simulations were run using individual based (VORTEX 9.72) and cohort-based (RAMAS GIS 4.0) programs. A baseline simulation allowed for the assessment of the status of the species based on estimates of extinction risk and population declines under current conditions of abundance and habitat availability. The role of multiple demographic, environmental, and anthropogenic parameters was evaluated to assess changes affecting population declines and extinction risk. The baseline simulation showed that the Blue-throated Macaw has a …
Unraveling The Determinants Of Protrusion Formation, Mita Varghese, Peter Gorsevski, Marilyn L. Cayer, Nancy S. Boudreau, Carol A. Heckman
Unraveling The Determinants Of Protrusion Formation, Mita Varghese, Peter Gorsevski, Marilyn L. Cayer, Nancy S. Boudreau, Carol A. Heckman
Biological Sciences Faculty Publications
A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the …
Extraction Of Dna From Plant And Fungus Tissues In Situ, Scott O. Rogers, Amal S. Abu Almakarem, Katie L. Heilman, Heather L. Conger, Yury M. Shtarkman
Extraction Of Dna From Plant And Fungus Tissues In Situ, Scott O. Rogers, Amal S. Abu Almakarem, Katie L. Heilman, Heather L. Conger, Yury M. Shtarkman
Biological Sciences Faculty Publications
Background: When samples are collected in the field and transported to the lab, degradation of the nucleic acids contained in the samples is frequently observed. Immediate extraction and precipitation of the nucleic acids reduces degradation to a minimum, thus preserving accurate sequence information. An extraction method to obtain high quality DNA in field studies is described. Findings. DNA extracted immediately after sampling was compared to DNA extracted after allowing the sampled tissues to air dry at 21°C for 48 or 72 hours. While DNA extracted from fresh tissues exhibited little degradation, DNA extracted from all tissues exposed to 21°C air …
Plasticity Of Total And Intracellular Phosphorus Quotas In Microcystis Aeruginosa Cultures And Lake Erie Algal Assemblages, Robert Michael Mckay, Matthew A. Saxton, Robert J. Arnold, Richard A. Bourbonniere, Steven W. Wilhelm
Plasticity Of Total And Intracellular Phosphorus Quotas In Microcystis Aeruginosa Cultures And Lake Erie Algal Assemblages, Robert Michael Mckay, Matthew A. Saxton, Robert J. Arnold, Richard A. Bourbonniere, Steven W. Wilhelm
Biological Sciences Faculty Publications
Blooms of the potentially toxic cyanobacterium Microcystis are common events globally, and as a result significant resources continue to be dedicated to monitoring and controlling these events. Recent studies have shown that a significant proportion of total cell-associated phosphorus (P) in marine phytoplankton can be surface adsorbed; as a result studies completed to date do not accurately report the P demands of these organisms. In this study we measure the total cell-associated and intracellular P as well as growth rates of two toxic strains of Microcystis aeruginosa Kütz grown under a range of P concentrations. The results show that the …
An Evaluation Of The Role Of Sensory Drive In The Evolution Of Lake Malawi Cichlid Fishes, Adam R. Smith, Moira J. Van Staaden, Karen L. Carleton
An Evaluation Of The Role Of Sensory Drive In The Evolution Of Lake Malawi Cichlid Fishes, Adam R. Smith, Moira J. Van Staaden, Karen L. Carleton
Biological Sciences Faculty Publications
Although the cichlids of Lake Malawi are an important model system for the study of sensory evolution and sexual selection, the evolutionary processes linking these two phenomena remain unclear. Prior works have proposed that evolutionary divergence is driven by sensory drive, particularly as it applies to the visual system.While evidence suggests that sensory drive has played a role in the speciation of Lake Victoria cichlids, the findings fromseveral lines of research on cichlids of LakeMalawi are not consistent with the primary tenets of this hypothesis. More specifically, three observations make the sensory drive model implausible in Malawi: (i) a lack …