Biology Commons^™

Isolation & Characterization Of Dna Polymerase Alpha And Gamma From Turnips (Brassica Rapa) And Etiolated Soybeans (Glycine Max), Donna Hill

Masters Theses & Specialist Projects

DNA polymerase alpha, the enzyme involved in nuclear DMA replication, and DNA polymerase gamma, the enzyme involved in organellular DNA replication, were isolated and purified from soybean and turnip. The enzymes were characterized following ammonium sulfate precipitation, DEAF-cellulcse, phosphocelluloca, and hydroxylapatite chromatography, and by non-denaturing polyacrylamide gel electrophoresis. Protein banct were electropluted and the enzymes characterized using kinetic studies and sensitivity to divalent cations and inhibitors. Molecular weight and subunit composition studies indicated a molecular weight for the catalytic subunit of DNA polymerase alpha in soybean and turnip to be 46kDa. DNA polymeraqe gamma was composed of a catalytic subunit …

The Short Term Effects Of Manganese Toxicity On Isocitrate Dehydrogenase In Wheat & Tobacco, Juergen Pfeiffer

Masters Theses & Specialist Projects

The short term effects of high manganese levels (80 mg/L) on wheat and tobacco isocitrate dehydrogenase (IDH) activity were compared. The change in activity was then compared to the concentrations of manganese in the tissues.

As the time of manganese exposure increased, the fresh weight IDH activities in wheat and tobacco decreased. In contrast both control groups had similar enzyme activities every 6 hr over the 24 hr test period. The decrease in IDH activity of wheat and tobacco plants in experimental groups seemed to be related to an increase of the manganese in the leaf tissue. The slower decrease …

Sulfur Accumulation In Xylem Tissue Of Forest Trees From Kentucky & Tennessee & Its Relation To Yearly Growth, Jennifer Sharp

Masters Theses & Specialist Projects

The levels of sulfur present in xylem tissue of Quercus alba (white oak) show a statistically significant increase from 1970 to 1983. This increase was found in trees from three different sites in Kentucky and Tennessee. The levels of sulfur present in Tsuga canadensis (hemlock) show a statistically significant increase from 1955 to 1970. Sensitivity, growth response to the tree's environment, decreased in oaks from 1933 to 1968 but increased slightly in hemlocks during the same time period. The data tend to support a direct relationship between increasing sulfur levels and a reduction in growth. Physiological and ecological studies of …

Ua66/6/2 Bionews From The Hill, Wku Biology

WKU Archives Records

Newsletter created by and about the WKU Biology department highlighting students, faculty and alumni activities.

Patterns Of Sulfur Deposition In The Wood Of Pinus Echinatia Mill. From The Cumberland Plateau In Kentucky, Darrell Ray

Masters Theses & Specialist Projects

Air pollution and acid precipitation have long had an influence on plant and animal life. In this study, the level of sulfur in the stem tissue of Pinus echinata Mill. was measured in trees from various sites along the western edge of the Cumberland Plateau to determine the presence and pattern of sulfur deposition in this species. Overall, a 40.28% increase in sulfur levels was observed from four sites along the plateau with levels increasing from 0.072 mgS/gdwt in the 1962-66 growth increment to 0.101 mg in the 1982-86 increment. According to a computer generated analysis of variance, the differences …

Sensory Stimuli Involved In Eliciting Agonistic Behavior In The Northern Fence Lizard, Sceloporus Undulatus Hyacinthinus, Laura Taylor

Masters Theses & Specialist Projects

Comparisons among sequence analyses of actions in response to various treatments of visual and olfactory cues were carried out in the lizard, Sceloporus undulatus hyacinthinus, to determine the relative roles of these cues in eliciting agonistic behavior. Visual cues were tested using mirrors, models and clear partitions between cages. Chemical cues were tested using the secretion from the cloaca of males. A combination of visual and olfactory cues was tested by streaking a mirror with the substance from the cloaca of a male. The responses to these treatment conditions were videotaped, and the transition frequencies between the most frequently …

Ua35/11 Wku Student Honors Research Bulletin, Wku University Honors Program

WKU Archives Records

The Western Kentucky University Student Honors Research Bulletin is dedicated to scholarly involvement and student research. These papers represent work done by students from throughout the university.

• Kesselring, Marcia. Attitudes Toward the Need for Computer Literacy
• Tuck, Janna & Karen Wiggins. Methylation and Confirmation of PGE
• Lewis, Gloria. John Donne's Attitude Toward Love
• Johnson, Linda. International Telecommunications Trade with Japan
• Sharpe, Greg. Precipitation Patterns in Bowling Green, Kentucky, 1980-1985
• Smith, Sandy. Religion and the Media: Alliance or War?
• Bell, Suzanne. Early Secret Involvement of the United States Military in Cambodia
• Scariot, Linda. Parental Divorce and Childhood Emotional Disturbances
• Daniel, Janice. …