Biology Commons^™

Grizzly Bears And Humans At Alpine Moth Sites In Wyoming, Usa, Erika A. Nunlist, Dan Tyers, Andrew Pils, Bok F. Sowell

Human–Wildlife Interactions

Army cutworm moths (Euxoa auxiliaris; moths) are an important seasonal higher elevational food source for grizzly bears (Ursus arctos horribilis; bears) in the Greater Yellowstone Ecosystem (Wyoming, Montana, and Idaho, USA). Increased human interaction with bears at moth sites is an important management issue because of the potential for displacing bears and the concern for human safety. Managers will need better information regarding human–bear interactions at high-density moth sites that are also accessible to humans to mitigate potential conflicts. In the summers of 2017 and 2018, we studied human–bear interactions at 2 of the most human …

Thermal Tolerance And Physiology Of Eleutherodactylus Coqui Along An Elevational Gradient In Hawai‘I, Karen H. Beard, Susannah S. French, Jack Marchetti

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The purpose of this study was to determine if the thermal tolerance and physiology of the coqui frog changed along HawaiÔiÕs elevation gradient. A short acclimation experiment was used to test the baseline thermal tolerance and physiology, and an extended acclimation experiment to test the acclimation ability of the thermal tolerance and physiology metrics of the coqui frogs collected from low (m), medium (350-550 m) and high elevations (>750 m).

Elevation Influences The Microhabitat Use And Baseline Physiology Of Coqui Frogs In Hawai‘I, Karen H. Beard, Susannah S. French, Jack Marchetti

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The purpose of this study was to determine whether elevation in HawaiÔi affects the coquiÕs microhabitat use such as substrate choice and height off the forest floor and physiological metrics such as osmolality, oxidative status, and energy metabolites (glucose, free glycerol, and triglycerides).

Avian Species Distribution Models: Using Location Data To Inform Management Decisions, Marilyn E. Wright

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Both state and federal wildlife agencies strive to conserve and protect wildlife and their habitats as an important public resource. Applied management decisions often rely on being able to obtain data that can efficiently and effectively enhance the understanding of these systems for informing management actions. Wildlife managers often focus efforts on a small subset of species from an ecosystem, typically called focal species, who can serve as surrogates for understanding the health and function of the system. Models that consider how these focal species interact with the ecosystem are often used to better understand important aspects of their life …

Bugs, Bullets And Birds: Factors Affecting The Health And Survival Of Ferruginous Hawk Nestlings In The Intermountain West, Ellis A. Juhlin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ferruginous Hawks are a bird of prey species that nest in sagebrush steppe and grassland habitat. These birds are threatened by habitat loss and fragmentation from urbanization, rural development, oil and natural gas extraction, habitat treatment projects, and wildfires, and experiencing widespread population declines across their breeding range. Because of this, Ferruginous Hawks (FEHAs) have been deemed a Species of Greatest Conservation Need by state management agencies in Utah, Idaho, Wyoming and Nevada. The health and survival of Ferruginous Hawk (FEHA) nestlings is crucial to long-term population viability. Understanding the threats these birds are facing as nestlings can provide valuable …

Insecticide Susceptibility And Resistance Detection In Phlebotomus Argentipes Sandflies (Diptera: Psychodidae: Phlebotominae), Shawna M. Hennings

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Leishmaniasis is an understudied disease found predominantly in high heat and humidity areas. The disease is transmitted by sandflies which are blood-feeding, biting insects. There is currently no known vaccine for any form of leishmaniasis and treatment imposes a significant economic impact on already poor and marginalized populations, as well as severe, lingering side effects for afflicted individuals. Annually, there are millions of people around the world afflicted with a form of Leishmaniasis that is transmitted by species of sandflies. The primary sandfly population control method for over half a century has been indoor residual spraying using insecticides. The selective …

Immunological Tradeoffs And The Impacts Of Urbanization On The Reproductive Ecology And Physiology Of The Side-Blotched Lizard (Uta Stansburiana), Emily E. Virgin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Investing resources into reproduction can limit energy available to other competing demands, such as fighting off an infection; yet, both processes are necessary for organisms to survive and pass on their genes to the next generation. These strategies often follow patterns associated with lifespan, such that shorter-lived animals are more likely to invest more resources into reproduction over survival, and vice versa in long-lived animals. However, environmental change caused by urbanization can disrupt these relationships, and the within- and transgenerational costs of urbanization on females and offspring are unknown. I address these uncertainties in three research chapters to better understand …

In Silico Analysis Of Tetrodotoxin Binding In Voltage-Gated Sodium Ion Channels From Toxin-Resistant Animal Lineages, Shana L. Geffeney, Jennie Ann Cordingley, Kenyon Mitchell, Charles T. Hanifin

Biology Faculty Publications

Multiple animal species have evolved resistance to the neurotoxin tetrodotoxin (TTX) through changes in voltage-gated sodium ion channels (VGSCs). Amino acid substitutions in TTX-resistant lineages appear to be positionally convergent with changes in homologous residues associated with reductions in TTX block. We used homology modeling coupled with docking simulations to test whether positionally convergent substitutions generate functional convergence at the level of TTX–channel interactions. We found little evidence that amino acids at convergent positions generated similar patterns among TTX-resistant animal lineages across several metrics, including number of polar contacts, polar contact position, and estimates of binding energy. Though binding energy …

An Evaluation Of Landscape, Climate, And Management Impacts On Bumble Bees (Hymenoptera: Apidae: Bombus) In Agroecosystems, Morgan Elizabeth Christman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bumble bees play pivotal roles in pollinating wild and cultivated plant communities. Unfortunately, bumble bee populations are declining due to disturbances such as landscape conversion and climate change. Additionally, traps used to monitor pest insect populations often capture bumble bees, leading to a concern that trap captures increase bumble bee mortality. First, I studied bumble bee communities based on land cover and weather variables in agricultural fields in Utah. Bumble bee communities were more diverse in agricultural fields with more agricultural land in the surrounding area, low temperatures, and high humidity during the growing season, and less diverse in fields …

Biodiversity And Foraging Preferences Of Bee Communities At Pinnacles National Park Over Time, Abigail M. E. Lehner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bees are considered to be the most important animal pollinator, providing billions of dollars in pollination services each year. Despite their importance in both natural and agricultural settings, the status of most native bees is unknown. Native bees are subject to a variety of threats including habitat loss, pesticide use, and climate change. Yet, monitoring programs have been implemented in few natural areas. Pinnacles National Park, PNP, in California is one of the only natural areas to have a large historical dataset on bees across decades with surveys conducted in 1996, 1997, 1998, 1999, 2002, 2011, and 2012. These surveys …

Natural History And Development Of Melittobia Acasta On Megachile Rotunda , Alan R. Anderson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Alfalfa is one of the most economically important crops in North America. To produce alfalfa seed, a pollinator must release plant reproductive organs by applying pressure to keel petals. The alfalfa leafcutting bee, Megachile rotundata, is the primary commercial pollinator of alfalfa. U.S. bee managers struggle to maintain populations due to many mortality factors. One cause is attack from parasitoid wasps such as Melittobia acasta. Females can lay hundreds of eggs and have multiple generations, which can decimate bee stocks. Chapter Two investigates the life cycle and base temperature that allows development of M. acasta on bee hosts. …

Maternal Effects And Management Of Alfalfa Leafcutting Bees, Makenna May Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Most bees are solitary and important contributors to pollination of a variety of crops. Solitary bees do not live in colonies. Instead, females work individually to construct nests and provision nutrients for each offspring. However, solitary bees can be difficult to manage, especially on a large scale like honey bees, because of differences in how species provision for their offspring and construct nests. Variations in the timing of offspring development to adulthood even within species makes commercial management difficult. Variations in reproductive strategy, like how many offspring to make, how much provision to give each, and when to make them …

Data From: Complex Tourism And Season Interactions Contribute To Disparate Physiologies In An Endangered Rock Iguana, Susannah French

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To promote survival and fitness, organisms use a suite of physiological systems to respond to both predictable and unpredictable changes in the environment. These physiological responses are also influenced by changes in life history state. The continued activation of physiological systems stemming from persistent environmental perturbations enable animals to cope with these challenges but may over time lead to significant effects on the health of wildlife. In the present study, we tested how varying environmental perturbations driven by tourism and associated supplemental feeding affects the energetics, corticosterone and immunity of six discrete populations of the northern Bahamian rock iguana (Cyclura …

Blood Chemistry And Biliverdin Differ According To Reproduction And Tourism In A Free-Living Lizard, Susannah French

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Changes in the physiological health of species are an essential indicator of changing conditions and environmental challenges. Reponses to environmental challenges can often induce stress, influence physiology, and change metabolism in organisms. Here we tested blood chemistry parameters indicative of stress and metabolic activity using an i-STAT point-of-care blood analyzer in seven populations of free-ranging rock iguanas exposed to varying levels of tourism and supplemental feeding. We found significant differences in blood chemistry (glucose, oxygen, carbon dioxide, hematocrit, hemoglobin, calcium, potassium, and biliverdin levels) among populations exposed to varying levels of tourism, and also some variation between sexes and reproductive …

Collaborative Research: From Cooperation To Exploitation: Context-Dependent Effects Of Nectar Microbes On Pollination Mutualisms, Robert Nelson Schaeffer

Funded Research Records

No abstract provided.

Social And Ecological Drivers Of Life History Evolution In Wild Bees, Karen Kapheim

Funded Research Records

No abstract provided.

Understanding The Drivers And Limiting Factors Of Tui Chub In Pyramid Lake, Nv As The Most Important Food Source For Recovering Lahontan Cutthroat Trout, Phaedra Budy

Funded Research Records

No abstract provided.

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preference, Hailey Hatch

Undergraduate Honors Capstone Projects

Floral microbes are an overlooked aspect of the extended floral phenotype. Through altering floral nectar chemistry, they can mediate interactions between flowers, pollinators, and other floral microbes, with significant implications for plant and pollinator health. Interactions between floral microbes and pollinators are critically important to understand, as pollinators provide important ecosystem services in both natural and agriculture systems. Here, I explored how floral nectar traits affected both evolution and competition within the floral yeast Metschnikowia reukaufii, the floral bacterium Bacillus subtilis, and other microbes isolated from Brassica rapa nectar, an important plant model system and oilseed crop. To …

Effect Of Basal Diet And Black Raspberry Supplementation On Gene Biomarkers Of "Leaky Gut" In A Mouse Model Of Colitis-Associated Colorectal Cancer, Eliza Catherine Stewart

Undergraduate Honors Capstone Projects

Colorectal cancer is currently the second leading cause of cancer death in the world. The risk of developing colorectal cancer increases significantly in individuals who suffer from colitis, which is inflammation of the colon lining, seen in Irritable Bowel Disease. The term “leaky gut” is often used to describe increased intestinal permeability and is closely related to colitis. Leaky gut is associated with dysbiosis of the gut microbiota, which can occur through diet. This dysbiosis leads to prolonged inflammation and affects genes that encode for proteins involved in the tight junctions between cells. Compromised intestinal integrity allows for translocation of …

Global Change Effects On Carbon Cycling In Terrestrial Ecosystems, Guopeng Liang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Since terrestrial ecosystems store approximately 3 times more carbon (C) than the atmosphere, they have a significant effect on the atmospheric CO₂ concentration. Although many studies have been conducted to determine global change effects on C cycling in terrestrial ecosystems, the underlying mechanisms remain uncertain. To address this knowledge gap, I utilized meta-analysis, laboratory experiments, and soil microbial community analysis.

In chapter 2, I conducted a meta-analysis to examine whether effects of long-term N addition on plant productivity can shift over time. I found that 44% of studies showed a marked trend (increase or decrease) in the strength of …

Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse

Undergraduate Honors Capstone Projects

Cloning is an important technique in functional genomics, particularly genetics and molecular biology. Cloned genes can be used to facilitate protein expression; this is an essential step in understanding gene function. In traditional cloning, the gene of interest is amplified and tagged with a restriction enzyme sequence and ligated to the digested open vector. However, the GATEWAY™ Cloning Technology provides a speedy and more efficient route to insert the gene of interest in the desired vector, known as Destination vectors or Expression vectors, using site-specific recombination sites and recombinase enzymes.¹ Once DNA segments from the gene of interest are …

Greater Sage-Grouse Brood Responses To Livestock Grazing In Sagebrush Rangelands, Hailey Peatross Wayment

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The distribution and abundance of the greater sage-grouse (Centrocercus urophasianus; sage-grouse) have declined in the last 60 years. Range contractions and population declines have been attributed to loss and fragmentation of their sagebrush (Artemisia spp.) habitats. Grazing by livestock remains the predominant anthropogenic land-use across sagebrush ecosystems in North America, occurring on 87% of remaining sage-grouse habitat. Most of the peer-reviewed literature reports the potential for negative impacts of sagebrush reduction treatments, to increase livestock forage, on sage-grouse habitat. However, few studies have linked livestock grazing at the landscape level to vital rates (e.g., nest initiation rates, …

Synthetic Hagfish Slime: Mechanical Characterization, Hayden Johns, Spencer Walker

Student Research Symposium

Hagfish are ancient animals that eject a strong slime when attacked by predators. The slime that the hagfish emit is composed of protein strands that cause it to have incredible strength. To defend against foes, the Navy launches plastic ropes into the propellers of enemy warships in order to decrease the thrust of the motors. In a push to find a more biodegradable solution, the utilization of hagfish slime has shown greatpromise in stopping propellers.We hope to understand how this remarkable biomaterial withstands the impact of a sharp and quickly rotating propeller, while simultaneously reducing the thrust of the propeller. …

Analysis Of The Relationship Between Oxytocin Receptor Mutations, Oxytocin Receptor Binding And Autism, Ethan Dayley

Student Research Symposium

Autism Spectrum Disorder (ASD) is a common neurological disorder with a wide range of symptoms, many of them social in nature. Although there is a large body on research regarding ASD, up until now there is no gene-brain-behavior link that has shown the exact cause of ASD. In 2018, Dr. Sara Freeman's research showed differences in oxytocin receptor (OXTR) binding density in two brain regions between typically developing and ASD individuals. Here we show that about 50% of the variation seen in Dr. Freeman's study can be explained by genetic variations (single nucleotide polymorphisms or SNPs) in the OXTR gene, …

Evolution Of Floral Microbes And The Resulting Effects On Pollinator Preferences, Hailey Hatch

Student Research Symposium

Floral microbes are an overlooked aspect of the extended floral phenotype. They mediate interactions between flowers, pollinators, and other floral microbes that have significant implications for pollinator and plant health by altering floral nectar chemistry. The interactions between floral microbes and pollinators are critically important to understand as pollinators have a key role in both the crop and natural ecosystem world. Our aim is to explore how floral nectar traits may affect evolution and competition within the floral yeast Metchnikowia reukaufii, the floral bacteria Bacillus subtilis, and other microbes isolated from Brassica rapa nectar. To address this, we are conducting …

Correction Of F508del And G542x Mutations In Sheep Fibroblasts Of Cystic Fibrosis Models Using Crispr/Cas9 And Piggybac, Kaden Bunch

Student Research Symposium

Cystic Fibrosis (CF) is a human genetic disease caused by mutations in the CF Transmembrane Conductance Regulator (CFTR) gene. Among the ~2000 known CF mutations, F508del mutation is found in 84% and G542X in 4.6% of the CF patients in US, respectively. The F508del mutation occurs in the exon 11 and is characterized by the deletion of the ‘CTT’ nucleotides that ultimately deletes the Phenylalanine residue at the position 508 of CFTR. It causes the misfolding of the CFTR protein, which is further degraded by proteases. The G542X is a nonsense mutation found in the exon 12 and associated with …

What Makes For A Good Silk?, Elijah Moss

Student Research Symposium

In Dr. Justin Jones' research lab, research is being conducted on different silks. The quality of the silk must be evaluated, and so I have identified three paragons that each silk strain is compared to: commercial viability, the efficacy of transgenic antimicrobial peptides, and tensile strength.

Phylogeny And Historical Biogeography Of Uroleucon Aphids, Audrey Mullen

Student Research Symposium

The aphid genus Uroleucon (Aphididae: Aphidinae: Macrosiphini) is a diverse group of about 230 species feeding mainly on Asteraceae as host plants and found globally in most temperate regions. However, the origins of the South American species are unknown, as are the relationships of these taxa to northern hemisphere Uroleucon species. This study seeks to establish the relationships between Uroleucon aphids native to South America and their North American and Eurasian counterparts using genetic data. We will use phylogenetic analysis of cytochrome c oxidase subunits 1 and 2 mitochondrial genes as well as the elongation factor 1α nuclear gene to …

Impacts Of Cftr Mutations On Pulmonary Development In Sheep, Kaden Bunch

Student Research Symposium

Due to various mutations, the loss of cystic fibrosis transmembrane conductance regulator (CFTR) function causes cystic fibrosis (CF), predisposing the lungs to chronic infection and inflammation. In young infants with CF, structural airway defects are increasingly recognized before the onset of significant lung disease, which suggests a developmental origin and a possible role in lung disease pathogenesis. The function of CFTR in lung development is unclear and developmental studies in humans with CF are not feasible. Young CF sheep show similar lung development and spontaneous postnatal lung disease similar to humans impacted by CF. We studied lung development in the …

Transgenic Mice Demonstrate The Level Of Contribution Of Protein Cd38 To Nad+ Decline Seen In Human Aging, Abby Tucker

Student Research Symposium

Niacin (NAD+) is a a vital molecule in human metabolism. NAD+ decline with age is well documented and low NAD+ levels may cause aging symptoms, however the molecular cause of NAD+ decline is poorly understood. Few pathways in the human metabolism fully consume NAD+ as it is usually recycled to NADH. One protein found on immune cells, called CD38, does fully consume NAD+ in its reaction, and so it may contribute to niacin deficiency by depleting the NAD+ in the body over time. To determine CD38’s role in causing niacin deficiency and aging, we knocked out the CD38 gene in …