Biology Commons^™

Sapl Annual Meeting 2012 : Dalliance With Dali's Ants, Deby L. Cassill, Nelson Poynter Memorial Library.

USF St. Petersburg Campus Faculty Publications

Speakers: Peter Tush, Curator of Education, Salvador Dali Museum; Deby Cassil, Ph.D. Associate Professor, USFSP.

Plant Species Richness And Species Area Relationships In A Florida Sandhill, Monica Ruth Downer

USF Tampa Graduate Theses and Dissertations

Pine sandhill are integral pyrogenic communities in the southeastern United States. Though once widespread, habitat destruction, fire suppression and fragmentation have reduced the population to nearly 3%. It is important to learn as much as possible about these unique areas in order to implement best management practices to conserve and restore the existing populations of these communities.

Fire is central to the maintenance of pine sandhill communities and two conceptual hypothesis regarding burn frequency have come to light in maintaining the unique species composition and richness of these areas. The first is the Intermediate Disturbance Hypothesis which suggests that intermediate …

Assessing The Link Between Coastal Development And The Quality Of Fish Habitat In Mangrove Tidal Tributaries, Justin Micheal Krebs

USF Tampa Graduate Theses and Dissertations

To assess the potential influence of coastal development on the quality of estuarine habitat for nekton, we characterized land use and the intensity of land development surrounding small tidal tributaries of Tampa Bay. Based on this characterization, we classified tributaries as undeveloped, industrial, urban or man-made (i.e., mosquito-control ditches). Over one-third (37%) of tributaries were determined to be heavily developed, while fewer than one-third (28%) remain relatively undeveloped. We then examined the nekton community from eleven tributaries in watersheds representing the defined land-use classes. Whereas mean nekton density and species richness were both independent of land use, nekton-community structure differed …

Matrix Metalloproteinase Genes Are Transcriptionally Regulated By E2f Transcription Factors: A Link Between Cell Cycle Control And Metastatic Progression, Jacqueline Lea Johnson

USF Tampa Graduate Theses and Dissertations

The RbµE2F transcriptional regulatory pathway plays a critical role in the cell cycle. Rb is inactivated through multiple waves of phosphorylation, mediated mainly by cyclin D and cyclin E associated kinases. Once Rb is inactivated, cells can enter Sµphase. Collectively, three Rb family members and ten E2F proteins coordinate every additional stage of the cell cycle, from quiescence to mitosis. However the RbµE2F pathway is frequently altered in cancer. Aside from cell proliferation, the RbµE2F pathway regulates other essential cellular processes including apoptosis, cell differentiation, angiogenesis and DNA damage repair pathways, but its role in invasion and cancer progression is …

University Of South Florida St. Petersburg Student Research Journal, Vol. 1, Issue 1, Article 5 : Geometric Probability Of Mating Success For The Greater Short-Nosed Fruit Bat, Cynopterus Sphinx, Krista Ford, Leon Hardy, Deby L. Cassill

USF St. Petersburg Campus Faculty Publications

In the bat Cynopterus sphinx, the random probability of mating success was calculated to be 4%. A combination of several adaptations dramatically increases their mating success to nearly 100%. First, the male and female hang upside down in a front-to-back mount. From behind, the male positions his penis dorsoventral toward the female's genitalia. The male maintains a tight hold on the female by biting the scruff on her neck and by holding her wings with his thumbs, allowing the pair to move forwards and backwards uninterruptedly and rhythmically. The male inserts the glans of his penis while the female bends …

Consequences Of Kleptoplasty On The Distribution, Ecology, And Behavior Of The Sacoglossan Sea Slug, Elysia Clarki, Michael Louis Middlebrooks

USF Tampa Graduate Theses and Dissertations

The sacoglossan sea slug Elysia clarki is able to photosynthesize for three to four months using chloroplasts sequestered from its algal food sources. Furthermore, the slug is able to store multiple chloroplasts from different algal species within the same cell. This research, consisting of several related studies, explores the role that provision of organic nutrients via photosynthesis plays in the biology of the slug. The first chapter demonstrates that, under conditions of starvation, photosynthetic activity in E. clarki remains fully functional for one month after which it then declines. During the first month of starvation the slug exhibits similar feeding …

Use Of Adventitious Roots For The Determination Of Hydroperiod In Isolated Wetlands, Michael Joseph Reyes

USF Tampa Graduate Theses and Dissertations

Abstract

Accurate measurement of the hydroperiod in isolated wetlands currently relies upon the installation and frequent monitoring of devices such as piezometers and staff gauges. Observations of biological indicators of the hydroperiod may be able to supplement data collected from these devices and could potentially replace them as a means of accurately determining this hydrologic interval. The study objective was to determine whether adventitious root formation and maturation on buttonbush (Cephalanthus occidentalis) could be used as a viable indicator of the hydroperiod in isolated wetlands. Buttonbush seedlings were flooded in a controlled environment over a three month period in the …

Stable Carbon Isotope Discrimination By Rubisco Enzymes Relevant To The Global Carbon Cycle, Amanda J. Boller

USF Tampa Graduate Theses and Dissertations

Five different forms of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO; IA, IB, IC, ID, II), the carboxylase of the Calvin-Benson-Bassham cycle (CBB), are utilized by plants, algae and autotrophic bacteria for carbon fixation. Discrimination against ¹³C by RubisCO is a major factor dictating the stable carbon isotopic composition (δ¹³C = {[¹³C/¹²C sample/¹³C/¹²C standard] - 1} X 1000) of biomass. To date, isotope discrimination, expressed as ε values (={[¹²k/¹³k] - 1} X 1000; ¹²k and ¹³k …

Targeting Α4 Integrin Containing Complexes In Multiple Myeloma Using Peptidomimetics, Michael Foster Emmons

USF Tampa Graduate Theses and Dissertations

In our previous work we demonstrated that the integrin antagonist, HYD1, induced necrotic cell death in myeloma cell lines in vitro and in vivo as a single agent. In order to further delineate biomarkers of response to HYD1 we developed an isogenic drug resistant variant named H929-60. We show that the acquisition of resistance towards HYD1 correlates with reduced expression of the cleaved α4 integrin subunit and beta 1 integrin. Moreover, we demonstrate that HYD1 interacts with α4 integrin in myeloma cells. Consistent with reduced VLA-4 expression, the resistant variant showed ablated functional binding to fibronectin, VCAM-1 and the bone …

Responses To Chemical Exposure By Foraminifera: Distinguishing Dormancy From Mortality, Benjamin James Ross

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout in 2010 released an estimated 4.9 million barrels of crude oil into the Gulf of Mexico in the 83 days between the initial explosion and the capping of the well. Response included extensive use of Corexit© oil dispersant. Although South Florida was spared exposure by currents, this event highlights the need for effective bioassay organisms for coral reefs. Amphistegina spp. are benthic foraminifers that host diatom symbionts in a relationship similar to that of coral and their zooxanthellae. Amphistegina spp. occur abundantly in reef communities nearly worldwide, are easily collected and maintained in culture, and are …

Management In A Neotropical Show Cave: Planning For Invertebrates Conservation, Thais Giovannini Pellegrini, Rodrigo Lopes Ferreira

International Journal of Speleology

Lapa Nova is a dolomitic cave about 4.5 km long located in northwestern Minas Gerais state, Brazil. The cave experiences intense tourism, concentrated over a single period of the year, during the Feast of Our Lady of Lapa. In order to evaluate the impacts felt by the invertebrate community from this tourism, a new methodology was proposed. Four types of areas (intense visitation area, outlying visitation areas, moderate visitation areas and no-visitation areas) were sampled for invertebrates. There was one sampling prior and another on the last day of the 128^th feast, to evaluate the effects of visitation on …

Aging Of Florida Blue Crabs, Callinectes Sapidus, Through The Biochemical Extraction Of Lipofuscin, Claire Elizabeth Crowley

USF Tampa Graduate Theses and Dissertations

The blue crab, Callinectes sapidus, represents an ecologically and economically important component of marine and estuarine ecosystems. In Florida, blue crab landings accounted for $9.6 million dollars during the 2010 fishing season. Accurate stock assessments for this valuable fishery are essential. Age is a critical biological component of accurate stock assessments; however, blue crabs and other crustaceans are especially difficult to age because of the complex nature of discrete growth. Biochemical extraction of an aging pigment, lipofuscin, was developed using blue crab eyestalks. The current study investigated the effects of freezing preservation on lipofuscin extracts and examined whether the …

Demasculinization And Feminization Of Male Gonads By Atrazine: Consistent Effects Across Vertebrate Classes, Krista A. Mccoy

Integrative Biology Faculty and Staff Publications

Atrazine is the most commonly detected pesticide contaminant of ground water, surface water, and precipitation. Atrazine is also an endocrine disruptor that, among other effects, alters male reproductive tissues when animals are exposed during development. Here, we apply the nine so-called “Hill criteria” (Strength, Consistency, Specificity, Temporality, Biological Gradient, Plausibility, Coherence, Experiment, and Analogy) for establishing cause–effect relationships to examine the evidence for atrazine as an endocrine disruptor that demasculinizes and feminizes the gonads of male vertebrates. We present experimental evidence that the effects of atrazine on male development are consistent across all vertebrate classes examined and we present a …

University Of South Florida St. Petersburg Student Research Journal, Vol. 1, Issue 1, Article 3 : Modeling A Molecular Dynamic Simulation Of The Stat3 Monomer, Jessica Ruekberg, Demetrios Kiriopoulos, Kevin Moore, Deby L. Cassill, Leon Hardy

USF St. Petersburg Campus Faculty Publications

STAT3, also known as signal transducer and activator of transcription 3, is a DNA transcription enzyme. In mammals including humans, STAT3 regulates the expression of a variety of genes that play a role in embryonic development. Specifically, STAT3 regulates cell division, cell differentiation and cell death (apoptosis). During embryonic development, STAT3 proteins are phosphorylated in a cell's cytoplasm in response to chemical growth factors. Once phosphorylated, STAT3 proteins pair up to form homodimers that act like pliers. STAT3 homodimers are transferred into the cell nucleus, where they bind to DNA to regulate embryonic development. When mutations of the STAT3 gene …

Blood-Brain Barrier Impairment In An Animal Model Of Mps Iii B, Svitlana N. Garbuzova-Davis, Michael Louis, Edward M. Haller, Hiranya M. Derasari, Ashley E. Rawls, Paul R. Sanberg

Integrative Biology Faculty and Staff Publications

Background: Sanfilippo syndrome type B (MPS III B) is caused by a deficiency of α-N-acetylglucosaminidase enzyme, leading to accumulation of heparan sulfate within lysosomes and eventual progressive cerebral and systemic multiple organ abnormalities. However, little is known about the competence of the blood-brain barrier (BBB) in MPS III B. BBB dysfunction in this devastating disorder could contribute to neuropathological disease manifestations.

Methodology/Principal Findings: In the present study, we investigated structural (electron microscope) and functional (vascular leakage) integrity of the BBB in a mouse model of MPS III B at different stages of disease, focusing on brain structures known to experience …

Computational Discovery Of Phenotype Related Biochemical Processes For Engineering, Andrea M. Rocha

USF Tampa Graduate Theses and Dissertations

Application of bioengineering technologies for enhanced biological hydrogen production is a promising approach that may play a vital role in sustainable energy. Due to the ability of several naturally occurring microorganisms to generate hydrogen through varying metabolic processes, biological hydrogen has become an attractive alternative energy and fuel source.

One area of particular interest is the production of biological hydrogen in organically-rich engineered systems, such as those associated with waste treatment. Despite the potential for high energy yields, hydrogen yields generated by bacteria in waste systems are often limited due to a focus on microbial utilization of organic material towards …

A Novel Device For Cell-Cell Electrofusion, Justin T. Stewart

USF Tampa Graduate Theses and Dissertations

Cell transplantation therapy is a potentially powerful tool and can be used to replace defective cells with healthy cells. This offers the possibility of alleviating the destructive symptoms for many diseases such as Parkinson's disease, Alzheimer's disease, stroke, spinal cord trauma, Type I diabetes and many more. While there are many diseases that could be positively impacted from cell transplantation therapy, the focus of this research is insulin dependent, Type I Diabetes.

The Islets of Langerhans are composed of various types of cells located in the pancreas and are responsible for a variety of biochemical functions. Specifically, the beta Islet …

The Conservation And Ecology Of Carnivorous Plants, David Jennings

USF Tampa Graduate Theses and Dissertations

As discussed in Chapter 1, although our understanding of the ecology and evolution of carnivorous plants has greatly improved in recent years, many fundamental questions remain unanswered. Unfortunately, at the present time, many carnivorous plants are increasingly threatened by anthropogenic activities. Indeed, over half of the carnivorous plant species assessed by the International Union for the Conservation of Nature (IUCN) are listed as `threatened', but the threats to carnivorous plants have not previously been quantified. In Chapter 2, I quantified the conservation threats to carnivorous plant taxa worldwide by searching peer-reviewed literature, and found data on the threats to 48 …

Seagrass Patch Dynamics In Areas Of Historical Loss In Tampa Bay, Fl, Usa, Kristen A. Kaufman

USF Tampa Graduate Theses and Dissertations

The study documents seagrass patch dynamics over large spatial extents in Tampa Bay, Florida. Using GIS techniques a set of fine scale seagrass maps was created within locations previously identified as "patchy" seagrass or areas of seagrass loss. Thirty randomly selected landscape windows of various extents were mapped for the years 2004, 2006, and 2008 by visualizing 0.3 m resolution color imagery on-screen at a digitizing scale of 1:500 using a minimum mapping unit of 1 m². Characteristics of seagrass patches and patterns of seagrass change were quantified using area-based and time interval metrics including total seagrass area, …

Underwater Hearing In The Loggerhead Turtle (Caretta Caretta): A Comparison Of Behavioral And Auditory Evoked Potential Audiograms, Kelly Martin

USF Tampa Graduate Theses and Dissertations

Methods for collecting behavioral audiograms are often time consuming and require trained, captive subjects. It is more practical to measure hearing sensitivity using electrophysiological methods, such as auditory evoked potential (AEP) testing, in which electrodes measure action potentials in response to acoustic stimuli. These data can be collected in a matter of hours. However, results should be verified through behavioral testing. Current knowledge of marine turtle auditory abilities is based on a few electrophysiological tests. The purpose of this study was to collect and compare behavioral and auditory evoked potential audiograms in a captive adult loggerhead turtle (Caretta caretta …

Population Genetics Of Amphiuma Means And Siren Lacertina In Central Florida, Anna C. Deyle

USF Tampa Graduate Theses and Dissertations

Population structure can be defined as a difference in the frequencies of alleles among populations, and it is often caused by barriers to dispersal. I used mitochondrial DNA sequences to examine the population structure of two large, aquatic salamanders, Amphiuma means (the Two-Toed Amphiuma) and Siren lacertina (the Greater Siren), at two spatial scales in peninsular Florida. Both species appear to have similar life histories, including poor dry land dispersal capabilities, and I hypothesized that they would exhibit significant population structure at both broad and fine scales. Wetland loss and landscape fragmentation are certain to have caused decreases in abundance …

Evaluating The Effects Of Beach Nourishment On Loggerhead Sea Turtle (Caretta Caretta) Nesting In Pinellas County, Florida, Corey R. Leonard Ozan

USF Tampa Graduate Theses and Dissertations

The health of Florida's beaches are vital to the survival of loggerhead sea turtles (Caretta caretta), as nearly half of the world's loggerheads nest on the states beaches. Many of the beaches utilized by the turtles have undergone nourishment projects in hopes of combating erosion of the shoreline, protecting beachfront property, and creating more suitable beaches for tourism. Although it is argued that beach nourishment benefits sea turtles by providing more nesting habitat, the effects of the Pinellas County nourishment projects on loggerhead nesting are unknown. Beach nourishment can alter the compaction, moisture content, and temperature of the sand, all …

The Roles Of Elevated Bcl-2 In Ovarian Cancer, Nicole Shree Anderson

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) is the second most common gynecologic cancer; however it is responsible for the most gynecologic cancer-related deaths. Apoptosis evasion is an important mechanism in OC tumorigenesis, and the prototypic anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), is often overexpressed in OC tumors. Gaining a better understanding of the mechanism(s) behind Bcl-2 overexpression and potential extra-anti-apoptotic functions of Bcl-2 could elucidate the importance of elevated Bcl-2 in OC. In the current study, I show through immunohistochemical analysis of normal, benign, and OC tissue sections, that both epithelial and stromal Bcl-2 expression decreases with OC progression. However, the number of …

Discrimination Of Human And Non-Human Sources Of Pollution In Gulf Of Mexico Waters By Microbial Source Tracking Methods And The Investigation Of The Influence Of Environmental Factors On Escherichia Coli Survival, Asja Korajkic

USF Tampa Graduate Theses and Dissertations

Water quality worldwide is assessed by enumeration of fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli, and enterococci) intended to act as surrogates for human enteric pathogens. In environmental waters, this predictive relationship is confounded by many possible sources of FIB with varying implications for human health. Many physico-chemical and biological factors influence the fate of enteric pathogens and FIB in aquatic habitats, but are poorly understood, thus limiting our understanding of the usefulness of FIB as fecal pollution indicators.

These studies explored the field application of a “toolbox” approach to microbial source tracking (MST) intended to discriminate between human …

Evolution Of The Hammerhead Cephalofoil: Shape Change, Space Utilization, And Feeding Biomechanics In Hammerhead Sharks (Sphyrnidae), Kyle Reid Mara

USF Tampa Graduate Theses and Dissertations

The relationship between form and function is often used to elucidate the biological role of a structure. Hammerhead sharks offer a unique opportunity to study form and function through phylogeny. Because sphyrnid sharks display a range of cranial morphologies this group can be used to address questions about the evolution of cranial design and investigate the effects of changes in head morphology on feeding structures and bite force. Geometric morphometrics, volumetric analyses, morphological dissections, and phylogenetic analyses of the cephalofoil were used to gain insight into changes in cranial design through evolutionary history. External morphometrics and internal volumetric analyses indicated …

Chk2 Is Negatively Regulated By Protein Phosphatase 2a, Alyson Freeman

USF Tampa Graduate Theses and Dissertations

Checkpoint kinase 2 (CHK2) is an effector kinase of the DNA damage response pathway and although its mechanism of activation has been well studied, the attenuation of its activity following DNA damage has not been explored. Here, we identify the B'α subunit of protein phosphatase 2A (PP2A), a major protein serine/threonine phosphatase of the cell, as a CHK2 binding partner and show that their interaction is modulated by DNA damage. B'α binds to the SQ/TQ cluster domain of CHK2, which is a target of ATM phosphorylation. CHK2 is able to bind to many B' subunits as well as the PP2A …

Survival Of The Misfits : Why Most Sperm Are Duds & Other Interesting Tales Of Evolution, Deby L. Cassill, Nelson Poynter Memorial Library.

USF St. Petersburg Campus Faculty Publications

Speaker: Dr. Deby Cassill

Evidence Of Compromised Blood-Spinal Cord Barrier In Early And Late Symptomatic Sod1 Mice Modeling Als, Svitlana N. Garbuzova-Davis, Samuel Saporta, Edward M. Haller, Irina Kolomey, Steven P. Bennett, Huntington Potter, Paul R. Sanberg

Integrative Biology Faculty and Staff Publications

Background: The blood-brain barrier (BBB), blood-spinal cord barrier (BSCB), and blood-cerebrospinal fluid barrier (BCSFB) control cerebral/spinal cord homeostasis by selective transport of molecules and cells from the systemic compartment. In the spinal cord and brain of both ALS patients and animal models, infiltration of T-cell lymphocytes, monocyte-derived macrophages and dendritic cells, and IgG deposits have been observed that may have a critical role in motor neuron damage. Additionally, increased levels of albumin and IgG have been found in the cerebrospinal fluid in ALS patients. These findings suggest altered barrier permeability in ALS. Recently, we showed disruption of the BBB and …

An Evaluation Of Movement Patterns And Effects Of Habitat Patch Size On The Demography Of The Florida Mouse (Podomys Floridanus), Irmgard Lukanik

USF Tampa Graduate Theses and Dissertations

Habitat degradation by humans has been the main reason for the decline in numbers of P. floridanus, the only mammal indigenous to the state of Florida, in the past century. The mouse inhabits what remains of scrub and sandhill associations, which are characterized by patches of sandy soils within a more mesic landscape. It has long been accepted that small populations are more prone to decline and extinction than are larger ones as a result of environmental fluctuations. I hypothesized that the demography of a population of P. floridanus would be affected by a restriction in numbers through habitat …

Community Composition Of Crustaceans And Gastropods On Caulerpa Prolifera, Halodule Wrightii And Thalassia Testudinum, Jennifer A. Gibson

USF Tampa Graduate Theses and Dissertations

No abstract provided.