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Full-Text Articles in Biology

Identification Of Microrna Biomarkers In Her2-Positive Breast Cancer, Hossam Tashkandi May 2014

Identification Of Microrna Biomarkers In Her2-Positive Breast Cancer, Hossam Tashkandi

Theses and Dissertations

Human epidermal growth factor receptor 2 (HER2) is overexpressed in approximately 30% of breast cancer patients with poor prognosis. In addition, microRNAs are small non-coding RNA that have been linked to many cancers. Here we investigate which miRNAs are differentially regulated by HER2 overexpression. Using quantitative reverse-transcription prolymerase chain reaction (QRT-PCR) and matching it with the clinical data acquired from Dvinge, we find five candidate miRNAs. When comparing the miRNAs’ effect on patient survival, only three miRNAs stand as good predictors of patient survival outcome. These miRNAs are miR-146a-5p, miR-181d, and miR-195-5p. When miR-146a-5p is up-regulated, which is the case …


Correction Of The Caulobacter Crescentus Na1000 Genome Annotation, Bert Ely, Latia Etheredge Scott Mar 2014

Correction Of The Caulobacter Crescentus Na1000 Genome Annotation, Bert Ely, Latia Etheredge Scott

Faculty Publications

Bacterial genome annotations are accumulating rapidly in the GenBank database and the use of automated annotation technologies to create these annotations has become the norm. However, these automated methods commonly result in a small, but significant percentage of genome annotation errors. To improve accuracy and reliability, we analyzed the Caulobacter crescentus NA1000 genome utilizing computer programs Artemis and MICheck to manually examine the third codon position GC content, alignment to a third codon position GC frame plot peak, and matches in the GenBank database. We identified 11 new genes, modified the start site of 113 genes, and changed the reading …


Multiple Roles For Hoxa3 In Regulating Thymus And Parathyroid Differentiation And Morphogenesis In Mouse, Jena L. Chojnowski, Kyoko Masuda, Heidi A. Trau, Kirk Thomas, Mario Capeechi, Nancy R. Manley Jan 2014

Multiple Roles For Hoxa3 In Regulating Thymus And Parathyroid Differentiation And Morphogenesis In Mouse, Jena L. Chojnowski, Kyoko Masuda, Heidi A. Trau, Kirk Thomas, Mario Capeechi, Nancy R. Manley

Natural Sciences Faculty Publications

Hoxa3 was the first Hox gene to be mutated by gene targeting in mice and is required for the development of multiple endoderm and neural crest cell (NCC)-derived structures in the pharyngeal region. Previous studies have shown that the Hoxa3 null mutant lacks third pharyngeal pouch derivatives, the thymus and parathyroids by E18.5, and organ-specific markers are absent or downregulated during initial organogenesis. Our current analysis of the Hoxa3 null mutant shows that organ-specific domains did undergo initial patterning, but the location and timing of key regional markers within the pouch, including Tbx1, Bmp4 and Fgf8, were altered. …


Art Or Science?, Allison Marsh Jan 2014

Art Or Science?, Allison Marsh

Section 3: Imaging the Fast Moving

No abstract provided.


Richard E. Petit, 1931-2013: A Brief Account Of His Malacological Contributions, Douglas A. Wolfe Ph.D. Jan 2014

Richard E. Petit, 1931-2013: A Brief Account Of His Malacological Contributions, Douglas A. Wolfe Ph.D.

Journal of the South Carolina Academy of Science

Abstract. R.E. Petit, a life member of the South Carolina Academy of Sciences, died on 31 December 2013. His remarkable scientific achievements as a self-trained malacologist are summarized, including an extensive list of named Molluscan taxa, mostly in the Cancellariidae, and a bibliography of his malacological publications


Modular Network Construction Using Eqtl Data: An Analysis Of Computational Costs And Benefits, Yen Yi Ho, Leslie M. Cope, Giovanni Parmigiani Jan 2014

Modular Network Construction Using Eqtl Data: An Analysis Of Computational Costs And Benefits, Yen Yi Ho, Leslie M. Cope, Giovanni Parmigiani

Faculty Publications

Background: In this paper, we consider analytic methods for the integrated analysis of genomic DNA variation and mRNA expression (also named as eQTL data), to discover genetic networks that are associated with a complex trait of interest. Our focus is the systematic evaluation of the trade-off between network size and network search efficiency in the construction of these networks. Results: We developed a modular approach to network construction, building from smaller networks to larger ones, thereby reducing the search space while including more variables in the analysis. The goal is achieving a lower computational cost while maintaining high confidence in …


Table Of Contents, Michele Harmon Dec 2013

Table Of Contents, Michele Harmon

Journal of the South Carolina Academy of Science

No abstract provided.


The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson Dec 2013

The Effects Of Salinity, Ph, Temperature, And Dissolved Oxygen On Sensitivity Of Pcr Identification Of T4 Bacteriophage, Joesph F. Cannon, Nicholas A. Thurn, Paul E. Richardson

Journal of the South Carolina Academy of Science

Bacteriophages are used as indicators of pathogenic bacteria in drinking, and wastewaters. They also show potential in limiting aquatic bacterial populations through their lytic properties. The effect of different water characteristics (salinity, pH, dissolved oxygen, and temperature) on the sensitivity of the PCR identification of virus particles were analyzed to determine at what levels bacteriophage can be detected in environmental samples. Results from this preliminary study indicate that a PCR bacteriophage detection technique has potential as a relatively efficient and economical indicator of coliform contamination in multiple aquatic environments. While further evaluation is needed, the protocol appears to function in …


Exploring The Mechanisms Of Allelopathic Interaction In The Invasive Annual Plant, Phyllanthus Urinaria, Alyssa L. Smith, Andrew R. Dyer Dec 2013

Exploring The Mechanisms Of Allelopathic Interaction In The Invasive Annual Plant, Phyllanthus Urinaria, Alyssa L. Smith, Andrew R. Dyer

Journal of the South Carolina Academy of Science

The belowground mechanisms facilitating invasion and proliferation of non-native plant species into natural communities are of critical importance for understanding plant invasions. Research on allelopathy suggests that some exotic plant species produce compounds via root exudates that may suppress or inhibit the growth of neighboring plants, but the specific mechanisms and consequences of these plant-chemical interactions remain elusive. In an effort to understand the abiotic and biotic factors governing allelopathic activity, a two-part greenhouse experiment was designed to examine plant-soil interactions of the invasive euphorb, Phyllanthus urinaria, and the residual soil effects of these interactions on subsequent growth of …


Dose Dependent Effects Of Caffeine On Cognitive Performance And Neuronal Activation, Stephan Albrecht, Helen Morris, Michelle Vieyra Dec 2013

Dose Dependent Effects Of Caffeine On Cognitive Performance And Neuronal Activation, Stephan Albrecht, Helen Morris, Michelle Vieyra

Journal of the South Carolina Academy of Science

Many students assume that the more caffeine you drink, the better your cognitive performance. Over-consumption of caffeine has many negative effects, so if there are no dose related cognitive benefits to large amounts of caffeine, then college students should limit their intake. This study looked at whether ingesting a medium dose (200 mg) versus a lower dose (100 mg) of caffeine improved short term memory as measured by Flanker and n-back tests, compared to a control group. In addition, we looked at whether larger doses of caffeine produced a difference in neuronal activation during these tests as measured by functional …


Fourier Analysis Of Phase Resetting Curves Of Neural Oscillators, Robert A. Raidt, Sorinel A. Oprisan Dec 2013

Fourier Analysis Of Phase Resetting Curves Of Neural Oscillators, Robert A. Raidt, Sorinel A. Oprisan

Journal of the South Carolina Academy of Science

We investigated the impact of changes in biologically relevant control parameters, such as the shape of an external perturbation or the conductance values of an individual model neuron, on the shape of the phase resetting curve (PRC) of that neuron. For that purpose, PRCs were generated for groups of Morris-Lecar (ML) model neurons with different conductance values but similar firing periods (within 0.005ms) using external rectangular, triangular, or trapezoidal perturbations of varying areas. These PRCs were numerically described and analyzed as a series of coefficient values using a Fourier Discrete Sine Transform (DST). We found that changes in the shape …


Experimental Demonstration Of Accelerated Extinction In Source-Sink Metapopulations, John M. Drake, Blaine D. Griffen Aug 2013

Experimental Demonstration Of Accelerated Extinction In Source-Sink Metapopulations, John M. Drake, Blaine D. Griffen

Faculty Publications

Population extinction is a fundamental ecological process which may be aggravated by the exchange of organisms between productive (source) and unproductive (sink) habitat patches. The extent to which such source-sink exchange affects extinction rates is unknown. We conducted an experiment in which metapopulation effects could be distinguished from source-sink effects in laboratory populations of Daphnia magna. Time-to-extinction in this experiment was maximized at intermediate levels of habitat fragmentation, which is consistent with a minority of theoretical models. These results provided a baseline for comparison with experimental treatments designed to detect effects of concentrating resources in source patches. These treatments showed …


2013 Annual Meeting Program Aug 2013

2013 Annual Meeting Program

Journal of the South Carolina Academy of Science

No abstract provided.


Aspergillosis Of Pseudopterogorgia Americana: Increased Host Range Of Aspergillus Sydowii From The Wider Caribbean, Garriet W. Smith Aug 2013

Aspergillosis Of Pseudopterogorgia Americana: Increased Host Range Of Aspergillus Sydowii From The Wider Caribbean, Garriet W. Smith

Journal of the South Carolina Academy of Science

Reports of gorgonian diseases have been increasing in the past few years,but, with the exception of Gorgonia spp., the etiological agents responsible are generally unknown. In the summer of 1999, populations of Pseudopterogorgia americana were observed with lesions and galls, somewhat similar to Aspergillus sydowii infections on Gorgonia ventalina, in Bermuda. Surveys of three sites were made, repeated the following two years, and compared with sites in the Bahamas. Microscopic observations of affected tissue and subsequent pure culture studies indicated the pathogen was also A. sydowii. Over half of the colonies at the Bahamas site were affected, but …


South Carolina Mayflies (Insecta: Ephemeroptera) Of Conservation Concern, Luke M. Jacobus Jul 2013

South Carolina Mayflies (Insecta: Ephemeroptera) Of Conservation Concern, Luke M. Jacobus

Journal of the South Carolina Academy of Science

Abstract: Nine mayfly species (Insecta: Ephemeroptera) that may be of conservation concern in South Carolina are discussed. Three such species associated with sand-bottomed streams are Acanthametropus pecatonica (Burks, 1953), Dolania americana Edmunds & Traver, 1959 and Homeoneuria dolani Edmunds, Berner & Traver, 1958. Three species of potential concern are associated with Hornleaf Riverweed (Podostemum ceratophyllum Michaux, 1803), and they include Barbaetis benfieldi Kennedy, 1985, Heterocloeon berneri (Muller-Liebenau, 1974) and Tsalia berneri (Allen & Edmunds, 1958). Mayflies of slow or stagnant waters that may be of conservation concern in South Carolina include Arthroplea bipunctata (McDunnough, 1924), Macaffertium lenati (McCafferty, 1990) …


Ernest Everett Just, Phd: Pioneer In Ecological Developmental (Eco-Devo) Biology, Katelyn M. Williams, Bryan A. Wilson, Wendi G. O'Connor, Monte S. Willis Jul 2013

Ernest Everett Just, Phd: Pioneer In Ecological Developmental (Eco-Devo) Biology, Katelyn M. Williams, Bryan A. Wilson, Wendi G. O'Connor, Monte S. Willis

Journal of the South Carolina Academy of Science

Ernest Everett Just, a pioneering American biologist, discovered the fundamental role of the environment in the development of embryos. His work led to the creation of the area of biology known as ecological developmental (Eco-Devo) biology. However, both his work and the context of his scientific contributions are not widely known. His work covered a diversity of fields of biology, including marine biology, cytology, and parthogenesis (asexual reproduction where growth and development of embryos occur without fertilization). His findings provided important concepts in developmental biology that are used to this day. Specifically, he demonstrated the importance of the cellular cytoplasm …


Sugar Promotes Vegetative Phase Change In Arabidopsis Thaliana By Repressing The Expression Of Mir156a And Mir156c, Li Yang, Mingli Xu, Yeonjong Koo, Jia He, R. Scott Poethig Mar 2013

Sugar Promotes Vegetative Phase Change In Arabidopsis Thaliana By Repressing The Expression Of Mir156a And Mir156c, Li Yang, Mingli Xu, Yeonjong Koo, Jia He, R. Scott Poethig

Faculty Publications

Nutrients shape the growth, maturation, and aging of plants and animals. In plants, the juvenile to adult transition (vegetative phase change) is initiated by a decrease in miR156. In Arabidopsis, we found that exogenous sugar decreased the abundance of miR156, whereas reduced photosynthesis increased the level of this miRNA. This effect was correlated with a change in the timing of vegetative phase change, and was primarily attributable to a change in the expression of two genes, MIR156A and MIR156C, which were found to play dominant roles in this transition. The glucose-induced repression of miR156 was dependent on the …


Pan-Arctic Distributions Of Continental Runoff In The Arctic Ocean, Cédric G. Fichot, Karl Kaiser, Stanford B. Hooker, Rainer M.W. Amon, Marcel Babin, Simon Bélanger, Sally A. Walker, Ronald Benner Jan 2013

Pan-Arctic Distributions Of Continental Runoff In The Arctic Ocean, Cédric G. Fichot, Karl Kaiser, Stanford B. Hooker, Rainer M.W. Amon, Marcel Babin, Simon Bélanger, Sally A. Walker, Ronald Benner

Faculty Publications

Continental runoff is a major source of freshwater, nutrients and terrigenous material to the Arctic Ocean. As such, it influences water column stratification, light attenuation, surface heating, gas exchange, biological productivity and carbon sequestration. Increasing river discharge and thawing permafrost suggest that the impacts of continental runoff on these processes are changing. Here, a new optical proxy was developed and implemented with remote sensing to determine the first pan-Arctic distribution of terrigenous dissolved organic matter (tDOM) and continental runoff in the surface Arctic Ocean. Retrospective analyses revealed connections between the routing of North American runoff and the recent freshening of …


Tnt1 Retrotransposon Mutagenesis: A Tool For Soybean Functional Genomics, Yaya Cui, Shyam Barampuram, Minviluz G. Stacey, C. Nathan Hancock, Seth Findley, Melanie Mathieu, Zhanyuan Zhang, Wayne A. Parrott, Gary Stacey Jan 2013

Tnt1 Retrotransposon Mutagenesis: A Tool For Soybean Functional Genomics, Yaya Cui, Shyam Barampuram, Minviluz G. Stacey, C. Nathan Hancock, Seth Findley, Melanie Mathieu, Zhanyuan Zhang, Wayne A. Parrott, Gary Stacey

Faculty Publications

Insertional mutagenesis is a powerful tool for determining gene function in both model and crop plant species. Tnt1, the transposable element of tobacco (Nicotiana tabacum) cell type 1, is a retrotransposon that replicates via an RNA copy that is reverse transcribed and integrated elsewhere in the plant genome. Based on studies in a variety of plants, Tnt1 appears to be inactive in normal plant tissue but can be reactivated by tissue culture. Our goal was to evaluate the utility of the Tnt1 retrotransposon as a mutagenesis strategy in soybean (Glycine max). Experiments showed that the …


Parsimony And Model-Based Analyses Of Indels In Avian Nuclear Genes Reveal Congruent And Incongruent Phylogenetic Signals, Tamaki Yuri, Rebecca T. Kimball, John Harshman, Rauri C.K. Bowie, Michael J. Braun, Jena L. Chojnowski, Kin-Lan Han, Shannon J. Hackett, Christopher J. Huddleston, William S. Moore, Sushma Reddy, Frederick H. Sheldon, David W. Steadman, Christopher C. Witt, Edward L. Braun Jan 2013

Parsimony And Model-Based Analyses Of Indels In Avian Nuclear Genes Reveal Congruent And Incongruent Phylogenetic Signals, Tamaki Yuri, Rebecca T. Kimball, John Harshman, Rauri C.K. Bowie, Michael J. Braun, Jena L. Chojnowski, Kin-Lan Han, Shannon J. Hackett, Christopher J. Huddleston, William S. Moore, Sushma Reddy, Frederick H. Sheldon, David W. Steadman, Christopher C. Witt, Edward L. Braun

Natural Sciences Faculty Publications

Insertion/deletion (indel) mutations, which are represented by gaps in multiple sequence alignments, have been used to examine phylogenetic hypotheses for some time. However, most analyses combine gap data with the nucleotide sequences in which they are embedded, probably because most phylogenetic datasets include few gap characters. Here, we report analyses of 12,030 gap characters from an alignment of avian nuclear genes using maximum parsimony (MP) and a simple maximum likelihood (ML) framework. Both trees were similar, and they exhibited almost all of the strongly supported relationships in the nucleotide tree, although neither gap tree supported many relationships that have proven …


The Effect Of Thymidylate Synthase Inhibitors On Bone Marrow Derived Cells In The Intestinal Tumor Microenvironment, Grishma Acharya Jan 2013

The Effect Of Thymidylate Synthase Inhibitors On Bone Marrow Derived Cells In The Intestinal Tumor Microenvironment, Grishma Acharya

Theses and Dissertations

Tumors have come to be known as independently functioning complex organs consisting of cancer cells coexisting with a heterogeneous mixture of host-derived non-neoplastic cells that form the tumor stroma. Tumor survival, progression, and metastasis depend on multiple close interactions between the cancer cells and the tumor stromal cells. These tumor stromal cells are mainly bone marrow derived cells (BMDCs) that are recruited to the primary tumor and sites of metastasis by a variety of signals secreted by the cancer cells. Because of the close interaction between the tumor and the tumor stroma, we propose that tumor stromal cells play an …


Quantifying The Effects Of Antibiotics Upon Benthic Diatoms And Marine Benthic Algal Communities, Isaac M. Hagenbuch Jan 2013

Quantifying The Effects Of Antibiotics Upon Benthic Diatoms And Marine Benthic Algal Communities, Isaac M. Hagenbuch

Theses and Dissertations

Antibiotics are a cornerstone of modern medicine. Their ability to impair prokaryotic metabolism has saved the lives of many millions of humans in the decades since wide antibiotic usage began. Antibiotic compounds are also widely used in the husbandry of animals that are used for food but, unfortunately, much of the antibiotic mass that goes into humans and animals is excreted unchanged. Those excretions go, directly or indirectly, into aquatic ecosystems. The discovery of this fact has led to a growing global concern about the fate of antibiotics in the environment. This concern is driven by no fewer than three …


Local Filters Limit Species Diversity, But Species Pools Determine Composition, Tara K. Rajaniemi, Deborah E. Goldberg, Roy Turkington, Andrew R. Dyer Dec 2012

Local Filters Limit Species Diversity, But Species Pools Determine Composition, Tara K. Rajaniemi, Deborah E. Goldberg, Roy Turkington, Andrew R. Dyer

Faculty Publications

In a series of three experiments, we tested for effects of species pools, resource stress, and species interactions on four aspects of community structure: species richness, evenness, species composition, and functional group composition. We also examined whether the impacts of species interactions on the community varied with resource availability or species pool. Communities of sand dune annuals grew from seed bank samples collected from two sites in three different years, so that the species pool differed at two levels: the source site and the year of seed bank collection. Communities experienced one of three irrigation treatments and a range of …


The Role Of Individual Behavior Type In Mediating Indirect Interactions, Blaine D. Griffen, Benjamin J. Toscano, John Gatto Aug 2012

The Role Of Individual Behavior Type In Mediating Indirect Interactions, Blaine D. Griffen, Benjamin J. Toscano, John Gatto

Faculty Publications

Trait-mediated indirect interactions (TMII) play an important role in structuring natural communities, and numerous studies have experimentally demonstrated their presence in a variety of systems. However, these studies have largely examined the presence or absence of traits that are responsible for these interactions, without considering natural variation between individuals in the extent to which these traits are manifested. We used a well-documented TMII to investigate the importance of individual behavior type for determining the strength of the TMII. The toadfish Opsanus tau has an indirect positive influence on bivalve survival because the mud crab Panopeus herbstii, a consumer of bivalves, …


A Plant Virus Substrate Induces Early Upregulation Of Bmp2 For Rapid Bone Formation, Pongkwan Sitasuwan, L. Andrew Lee, Peng Bo, Erin N. Davis, Yuan Lin, Qian Wang Apr 2012

A Plant Virus Substrate Induces Early Upregulation Of Bmp2 For Rapid Bone Formation, Pongkwan Sitasuwan, L. Andrew Lee, Peng Bo, Erin N. Davis, Yuan Lin, Qian Wang

Faculty Publications

Many nanoscale materials have been developed to investigate the effects on stem cell differentiations via topographical and chemical cues for applications in tissue engineering and regenerative medicine. The use of plant viruses as cell supporting substrates has been of particular interest due to the rapid induction of bone marrow derived mesenchymal stem cells (BMSCs) towards osteogenic cells. In this study, the role of Tobacco mosaic virus (TMV) and its early effects on osteoinduction with particular emphasis on the regulation of bone morphogenetic protein-2 (BMP2) was examined. We observed that the cells on the virus substrate immediately aggregated and formed bone-like …


Tracing The Transport Of Colored Dissolved Organic Matter In Water Masses Of The Southern Beaufort Sea: Relationship With Hydrographic Characteristics, A. Matsuoka, A. Bricaud, Ronald Benner, J. Para, R. Sempéré, L. Prieur, S. Bélanger, M. Babin Mar 2012

Tracing The Transport Of Colored Dissolved Organic Matter In Water Masses Of The Southern Beaufort Sea: Relationship With Hydrographic Characteristics, A. Matsuoka, A. Bricaud, Ronald Benner, J. Para, R. Sempéré, L. Prieur, S. Bélanger, M. Babin

Faculty Publications

Light absorption by colored dissolved organic matter (CDOM) [aCDOM(λ)] plays an important role in the heat budget of the Arctic Ocean, contributing to the recent decline in sea ice, as well as in biogeochemical processes. We investigated aCDOM(λ) in the Southern Beaufort Sea where a significant amount of CDOM is delivered by the Mackenzie River. In the surface layer, aCDOM(440) showed a strong and negative correlation with salinity, indicating strong river influence and conservative transport in the river plume. Below the mixed layer, a weak but positive correlation between aCDOM(440) and salinity was observed above the upper halocline, resulting from …


Understanding Fracture, Allison Marsh Jan 2012

Understanding Fracture, Allison Marsh

Section 3: Imaging the Fast Moving

No abstract provided.


Wall_1.6 — Acknowledgements, Allison Marsh Jan 2012

Wall_1.6 — Acknowledgements, Allison Marsh

Section 1: Introduction

No abstract provided.


Photoproduction Of Ammonium In The Southeastern Beaufort Sea And Its Biogeochemical Implications, H. Xie, S. Belanger, G. Song, Ronald Benner, A. Taalba, M. Blais, J. É. Tremblay, M. Babin Jan 2012

Photoproduction Of Ammonium In The Southeastern Beaufort Sea And Its Biogeochemical Implications, H. Xie, S. Belanger, G. Song, Ronald Benner, A. Taalba, M. Blais, J. É. Tremblay, M. Babin

Faculty Publications

Photochemistry of dissolved organic matter (DOM) plays an important role in marine biogeochemical cycles, including the regeneration of inorganic nutrients. DOM photochemistry affects nitrogen cycling by converting bio-refractory dissolved organic nitrogen to labile inorganic nitrogen, mainly ammonium (NH4+).During the August 2009 Mackenzie Light and Carbon (MALINA) Program, the absorbed photon-based efficiency spectra of NH4+ photoproduction (i.e. photoammonification) were determined using water samples from the SE Beaufort Sea, including the Mackenzie River estuary, shelf, and Canada Basin. The photoammonification efficiency decreased with increasing wavelength across the ultraviolet and visible regimes and was higher in offshore waters …


An Unbiased Approach To Identify Genes Involved In Development In A Turtle With Temperature-Dependent Sex Determination, Jena L. Chojnowski, Edward L. Braun Jan 2012

An Unbiased Approach To Identify Genes Involved In Development In A Turtle With Temperature-Dependent Sex Determination, Jena L. Chojnowski, Edward L. Braun

Natural Sciences Faculty Publications

Many reptiles exhibit temperature-dependent sex determination (TSD). The initial cue in TSD is incubation temperature, unlike genotypic sex determination (GSD) where it is determined by the presence of specific alleles (or genetic loci). We used patterns of gene expression to identify candidates for genes with a role in TSD and other developmental processes without making a priori assumptions about the identity of these genes (ortholog-based approach). We identified genes with sexually dimorphic mRNA accumulation during the temperature sensitive period of development in the Red-eared slider turtle (Trachemys scripta), a turtle with TSD. Genes with differential mRNA accumulation in response to …