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Full-Text Articles in Biology
A Rapid System For Analyzing Histone H4 Mutations In The Yeast Saccharomyces Cerevisiae, Edward Rex Lakey Harkrader
A Rapid System For Analyzing Histone H4 Mutations In The Yeast Saccharomyces Cerevisiae, Edward Rex Lakey Harkrader
Master's Theses
Genetically engineered, specific histone H4 mutations have been analyzed in yeast which lack normal H4 protein using the time consuming technique of tetrad dissection. The purpose of the present research project has been to develop a rapid new system for analyzing histone H4 mutations in yeast. The new system has made it possible to isolate quickly cells which are surviving on mutant H4 only. The system will make it possible to analyze more quickly large numbers of H4 mutations. It is termed a plasmid shuttle system because of the use of plasmid vectors for shuttling histone H4 alleles in and …
Sodium/Proton Exchange In The Gills Of Three Species Of Crabs : Libinia Dubia, Callinectes Sapidus, And Menippe Mercenaria, Nicholas J. Maiolo
Sodium/Proton Exchange In The Gills Of Three Species Of Crabs : Libinia Dubia, Callinectes Sapidus, And Menippe Mercenaria, Nicholas J. Maiolo
Master's Theses
Na+/H+ exchange was studied in plasma membrane vesicles from gills of three species of crabs: Callinectes sapidus (blue crab), Libinia dubia (spider crab), and Menippe mercenaria (stone crab). Spider crabs and stone crabs were acclimated to full (35ppt) seawater, while blue crabs were acclimated to both dilute (5ppt) and full strength seawater. Membrane vesicles were prepared by sucrose density centrifugation. Na+/H+ exchange was analyzed using acridine orange fluorescence. Male C. sapidus showed the most exchange activity (per g gill tissue), followed by M. mercenaria, female C. sapidus and Libinia dubia. No difference in exchange activity was evident when blue crabs …
A Reverse Genetics Approach For Studying Histone H4 Structure-Function Relationships In Yeast, Michael J. Bonaduce
A Reverse Genetics Approach For Studying Histone H4 Structure-Function Relationships In Yeast, Michael J. Bonaduce
Master's Theses
A previously constructed mutation of the histone H4 gene has been the subject of a detailed analysis. The mutation involves an H4 gene encoding two amino acid changes which substitute aspartic acid for glycine at positions 13 and 42 of the polypeptide. These substitutions result in a nonfunctional H4 protein when assayed in Saccharomyces cerevisiae. In contrast, an analysis of an amino terminal deletion of amino acids 3-26 results in a functional H4 protein. Physical biochemistry studies identify the sites of histone-histone interactions between residues 38-102 of histone H4, the same area in which position 42 resides. The question being …
Histology Of The Ovaries And Fat Bodies Of Chthonerpeton Indistinctum, Rafael O. De Sá, Nibia Berois
Histology Of The Ovaries And Fat Bodies Of Chthonerpeton Indistinctum, Rafael O. De Sá, Nibia Berois
Biology Faculty Publications
Histological analysis revealed three different stages in the development of ovarian follicles of Chthonerpeton indistinctum. These stages are identified by (1) location of the oocyte in the ovary wall, (2) oocyte morphology and staining characteristics, (3) development of the zona pellucida, and (4) organization of the follicular layer. Atretic follicles and corpora lutea also were found. Fat bodies are composed of well-developed adipose tissue.
Some Chemical And Physical Factors Influencing Growth Of Panus Stipticus Bulliard Ex Fries And Armillariella Mellea (Vahl Ex Fries) Karsten, James Robert Kirkland
Some Chemical And Physical Factors Influencing Growth Of Panus Stipticus Bulliard Ex Fries And Armillariella Mellea (Vahl Ex Fries) Karsten, James Robert Kirkland
Master's Theses
Two species of wood rotting fungi were used to investigate the possibility that growth-enhancing or inhibitory substances in woods from various trees are responsible for the distribution of these fungi in nature. Panus stipticus (Bulliard ex Fries) and Armillariella mellea (Vahl ex Fries) Karsten were cultured in the laboratory on blocks of wood from Taxodium distichum (L.) Richard, Juniperus virginiana L., Pinus taeda L. and Quercus rubra L. Panus stipticus grew well on the surface of the wood blocks; histological studies showed less penetration into the xylem of the softwoods than into that of Q. rubra. Armillariella mellea grew well …