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Articles 721 - 750 of 1681
Full-Text Articles in Biology
Effect Of Sodium Nitroprusside On Micropropagation And Biochemical Parameters Of Cab-6p And Gisela 6 Cherry Rootstocks, Virginia Sarropoulou, Kortessa Dimassi-Theriou, Ioannis Therios
Effect Of Sodium Nitroprusside On Micropropagation And Biochemical Parameters Of Cab-6p And Gisela 6 Cherry Rootstocks, Virginia Sarropoulou, Kortessa Dimassi-Theriou, Ioannis Therios
Turkish Journal of Biology
The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 $\mu$M 6-benzylaminopurine (BA) and SNP. Gisela 6 microcuttings treated with BA + 30 $\mu$M SNP gave the highest average shoot number (4.15) and multiplication percentage (100%). In CAB-6P, $\alpha$-naphthaleneacetic acid (NAA) + 30 $\mu$M SNP led to the maximum root number (13.79) and fresh weight (0.336 g); average root length (82 mm) and rooting percentage (95.45%) were greatest after adding 40 and 10 $\mu$M SNP + NAA, respectively. In Gisela 6, NAA + 40 …
Y Chromosome Genetic Diversity And Breed Relationships In Native Polish Cattle Assessed By Microsatellite Markers, Beata Prusak, Wioletta Sawicka-Zugaj, Agnieszka Korwin-Kossakowska, Tomasz Grzybowski
Y Chromosome Genetic Diversity And Breed Relationships In Native Polish Cattle Assessed By Microsatellite Markers, Beata Prusak, Wioletta Sawicka-Zugaj, Agnieszka Korwin-Kossakowska, Tomasz Grzybowski
Turkish Journal of Biology
Cattle provide a wide range of products and services to humans; thus the significance of conservation of livestock genetic resources is evident. We analyzed the genetic variability of five Y-chromosomal microsatellites in 395 bulls representing six cattle breeds (Polish Red, Polish Whitebacks, Polish Red-and-White, Polish Black-and-White, Polish Holstein Friesian, and Simmental). We identified three haplotypes in the paternal gene pool of analyzed populations. Haplotype diversity was low and frequencies ranged from 9.87% to 100%. In Polish autochthonous breeds (Polish Red, Polish Whitebacks), we detected two and three haplotypes. The network analysis revealed that tested bulls of both breeds were distributed …
Ghrelin Effects On Midgut Tissue Antioxidative Defense And Glutathione S-Transferase Activity In Lymantria Dispar (Lepidoptera), Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dragana Matic, Anja Gavrilovic, Aleksandra Mrkonja, Larisa Ilijin
Ghrelin Effects On Midgut Tissue Antioxidative Defense And Glutathione S-Transferase Activity In Lymantria Dispar (Lepidoptera), Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dragana Matic, Anja Gavrilovic, Aleksandra Mrkonja, Larisa Ilijin
Turkish Journal of Biology
The aim of the study was to examine changes in Cu?Zn superoxide dismutase (SOD), catalase (CAT), their gel electrophoresis profiles, glutathione reductase (GR) activity, amount of glutathione (GSH), and the activity of glutathione S-transferase (GST) - phase II biotransformation enzyme in the midgut tissue of gypsy moth (Lymantria dispar L. (Lepidoptera: Lymantriidae)) larvae after ghrelin treatment. Four subpicomolar injections of ghrelin (0.3 pmol) or physiological saline (control) were applied every 24 h. The SOD, CAT, GR, GST activity, and amount of GSH were higher in the ghrelin-treated group than in the control. Electrophoresis gel bands of SOD and CAT had …
The Biochemical And Histoanatomical Response Of Some Woody Species To Anthropic Impact In Suceava County, Romania, Elena Ciornea, Irina Boz, Elena Ionel, Sabina Ioana Cojocaru, Gabriela Dumitru
The Biochemical And Histoanatomical Response Of Some Woody Species To Anthropic Impact In Suceava County, Romania, Elena Ciornea, Irina Boz, Elena Ionel, Sabina Ioana Cojocaru, Gabriela Dumitru
Turkish Journal of Biology
The use of extractive mining technologies in various areas of Suceava, Romania (C?limani Mountains - sulfur, Tarni?a-Ostra - copper and barite, and Crucea-Botuşana - uranium) has resulted in the accumulation of surplus chemicals with direct action on the ecosystem. The aim of this study was to analyze the possible damage on some woody species (Picea abies L., Populus tremulaL., Salix alba L., Betula verrucosa Ehrh.,and Larix decidua Mill.) and their adaptive and phytoremediation capacity through the biomarkers of oxidative stress and histoanatomical observations. Thus, we determined the total soluble protein concentration and superoxide dismutase, catalase, and peroxidase activities. Cross-sections of …
Expression, Purification, And Characterization Of Recombinant Humanparaoxonase 1 (Rhpon1) In Pichia Pastoris, Yağmur Ünver, Esabi̇ Başaran Kurbanoğlu, Orhan Erdoğan
Expression, Purification, And Characterization Of Recombinant Humanparaoxonase 1 (Rhpon1) In Pichia Pastoris, Yağmur Ünver, Esabi̇ Başaran Kurbanoğlu, Orhan Erdoğan
Turkish Journal of Biology
The main purpose of the present study was to perform the expression in Pichia pastoris X-33 of the human paraoxonase 1 (hPON1) enzyme, which is a mammalian serum protein. Extracellular hPON1 enzyme was expressed with the pPICZ?A vector using a strong AOX promoter, and enzyme secretion in the fermentation medium was achieved by means of Saccharomyces cerevisiae alpha factor signal sequence. The recombinant cells were grown in a shaking flask containing production medium. SDS-PAGE and Western blot analysis illustrated that the molecular mass of extracellular hPON1 enzyme produced by the recombinant P. pastoris strain was 59.1 kDa. Biochemical characterization of …
Melatonin, Mitochondria, And Ca2+ Homeostasis In The Exocrine Pancreas: An Overview, Antonio Gonzalez, Patricia Santofimia-Castaño, Gines Maria Salido
Melatonin, Mitochondria, And Ca2+ Homeostasis In The Exocrine Pancreas: An Overview, Antonio Gonzalez, Patricia Santofimia-Castaño, Gines Maria Salido
Turkish Journal of Biology
Melatonin, a derivative of the amino acid tryptophan, was initially thought to be mainly produced and secreted by the pineal gland; with time, it was also found in other tissues and organs, and even in plants. Since its discovery, the study of the role of the indole in cellular homeostasis has generated impressive data, which have led researchers to a common idea regarding the positive actions of melatonin in health. The uncontrolled production of free radicals in cellular systems leads to the situation termed oxidative stress, which has been signaled as the basis of disease and aging. In the exocrine …
Role Of Melatonin On Calcium Signaling And Mitochondrial Oxidativestress In Epilepsy: Focus On Trp Channels, Mustafa Naziroğlu
Role Of Melatonin On Calcium Signaling And Mitochondrial Oxidativestress In Epilepsy: Focus On Trp Channels, Mustafa Naziroğlu
Turkish Journal of Biology
Calcium ion (Ca2+) accumulation and excessive oxidative stress in the hippocampus and brain cortex have long been known as major contributors to the etiology of epilepsy. I have reviewed the role of Ca2+ signaling through cation channels and mitochondria-mediated oxidative stress on epilepsy in human and animals. A review of the relevant papers and results from recent studies were obtained from PubMed and the Science Citation Index. Current literature findings indicate that melatonin and agomelatine reduce activation of hippocampal transient receptor potential (TRP), glutamate receptors, and voltage-gated calcium channels that are critical for the development of abnormal Ca2+ homeostasis and …
Melatonin As A Stabilizer Of Mitochondrial Function: Role In Diseases And Aging, Cristina Carrasco, Ana B. Rodriguez, Jose A. Pariente
Melatonin As A Stabilizer Of Mitochondrial Function: Role In Diseases And Aging, Cristina Carrasco, Ana B. Rodriguez, Jose A. Pariente
Turkish Journal of Biology
It is now almost 60 years since the discovery of melatonin and new physiological functions of the indole continuously appear in the most recent studies worldwide. Experimental evidence emphasizes its importance as a stabilizer of the mitochondrial bioenergetics, which could be related to the prevention of development of aging and several diseases. In the next years, conscientious investigation about this topic should be undertaken by scientists of different research areas to achieve a better understanding of the molecular mechanisms implied. This will ultimately allow the development and clinical application of efficacious treatments.
Prooxidant Effects Of Melatonin: A Brief Review, Malwina S. Munik, Cem Ekmekçi̇oğlu
Prooxidant Effects Of Melatonin: A Brief Review, Malwina S. Munik, Cem Ekmekçi̇oğlu
Turkish Journal of Biology
Melatonin acts classically through the widely expressed G protein-coupled membrane receptors MT1 and MT2, respectively. The functional role of the MT receptors is not fully clear with multiple effects, such as effects on the circadian, reproductive, immune, cardiovascular, and intestinal systems, being suggested. In addition to receptor-mediated effects, melatonin also acts as a potent antioxidant and it is quite evident that melatonin and its metabolites efficiently reduce oxidative damage to proteins, lipids, and DNA and also exert protective effects on mitochondria. The potent antioxidant activity of melatonin stems from various complex mechanisms, including direct scavenging of radicals, stimulation of antioxidant …
Mitochondrial Dna-Related Disorders: Emphasis On Mechanisms And Heterogeneity, Umut Cagin, Jose Antonio Enriquez
Mitochondrial Dna-Related Disorders: Emphasis On Mechanisms And Heterogeneity, Umut Cagin, Jose Antonio Enriquez
Turkish Journal of Biology
Mitochondrial diseases are a heterogeneous group of disorders that are currently the focus of intense research. The many cell functions performed by mitochondria include ATP production, calcium homeostasis, and apoptosis. One of the unique properties of mitochondria is the existence of a separate mitochondrial genome (mitochondrial DNA, mtDNA) found in varying copy numbers and containing 37 genes, 13 of them encoding proteins. All 13 mitochondrially encoded proteins form part of oxidative phosphorylation complexes through combination with approximately 100 nuclear DNA-encoded proteins. Coregulation of nDNA and mtDNA is therefore essential for mitochondrial function, and this coregulation contributes to the heterogeneity and …
Expression Profiles Of Genes Related To Melatonin And Oxidative Stress In Human Renal Proximal Tubule Cells Treated With Antibiotic Amphotericin B And Its Modified Forms, Joanna Gola, Aleksandra Skubis, Bartosz Sikora, Celina Kruszniewska-Rajs, Jolanta Adamska, Urszula Mazurek, Barbara Strzalka-Mrozik, Grzegorz Czernel, Mariusz Gagos
Expression Profiles Of Genes Related To Melatonin And Oxidative Stress In Human Renal Proximal Tubule Cells Treated With Antibiotic Amphotericin B And Its Modified Forms, Joanna Gola, Aleksandra Skubis, Bartosz Sikora, Celina Kruszniewska-Rajs, Jolanta Adamska, Urszula Mazurek, Barbara Strzalka-Mrozik, Grzegorz Czernel, Mariusz Gagos
Turkish Journal of Biology
Melatonin protects cells from oxidative stress caused by antibiotics. Through the generation of reactive oxygen species (ROS), amphotericin B (AmB) causes oxidative injuries of the kidneys and liver. Generation of oxidized forms of AmB (AmB-ox) in a patient's circulation may also generate ROS, causing side effects. Studies aimed at elimination of toxic properties of AmB are focused on forming complexes of AmB with copper(II) ions (AmB-Cu2+). However, the influence of such modified forms on renal cells and their transcriptome has not been studied so far. Therefore, the aim of this study was to answer the question of whether AmB-Cu2+ complexes …
The Effects Of Midazolam And Etomidate On The Antioxidant System In The Rat Liver, Di̇lek Bayram, Meral Öncü, Efkan Uz, Haci Ramazan Yilmaz, Ayşe Yi̇ği̇t, Ahmet Koçak
The Effects Of Midazolam And Etomidate On The Antioxidant System In The Rat Liver, Di̇lek Bayram, Meral Öncü, Efkan Uz, Haci Ramazan Yilmaz, Ayşe Yi̇ği̇t, Ahmet Koçak
Turkish Journal of Biology
This study was conducted to investigate the histopathological and biochemical effects of midazolam and etomidate, which are intravenous anesthetics used in surgical operations, on rat liver. There were 3 study groups each consisting of 9 animals: the control, midazolam, and etomidate groups. Midazolam and etomidate were administered 10 times with 1-day intervals. At the end of the study, tissues were taken for histological examination and biochemical analysis. We determined superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels in the liver. There was a slight increase in MDA level in the midazolam-exposed group when compared with the controls, and this …
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Turkish Journal of Biology
Acrylamide (AA) has neurotoxic, mutagenic, and genotoxic effects in humans and experimental animals. Fruit consumption is important for human health, because fruits are the source of many nutrients such as vitamins, minerals, carotenoids, dietary fiber, and phytonutrients. Many agricultural products provide natural melatonin in the diet. At the onset of the study, rats were weighted and randomly divided into four groups each containing 10 rats as follows: group 1: control (fed with normal diet and normal drinking water); group 2: apricot (fed with a daily diet with 5% apricot and normal drinking water); group 3: AA (administered daily acrylamide at …
Melatonin Induces Antiproliferative Activity Through Modulation Of Apoptoticpathway In H-Ras Oncogene Transformed 5rp7 Cells, Ayşe Kaplan, Gülşen Akalin Çi̇ftçi̇, Hati̇ce Mehtap Kutlu
Melatonin Induces Antiproliferative Activity Through Modulation Of Apoptoticpathway In H-Ras Oncogene Transformed 5rp7 Cells, Ayşe Kaplan, Gülşen Akalin Çi̇ftçi̇, Hati̇ce Mehtap Kutlu
Turkish Journal of Biology
In the present study, cytotoxic and apoptotic effects of melatonin were investigated on H-ras oncogene transformed rat embryo fibroblast 5RP7 cells. Melatonin inhibited cell growth and induced apoptosis in 5RP7 cells. We showed inhibition of cell proliferation that was time- and dose-dependent in 5RP7 cells for 24 and 48 h. Melatonin was not toxic in NIH/3T3 primary mouse embryonic fibroblast cells at low doses for 24 and 48 h. The IC50 (380 μM) value of melatonin for 48 h was chosen for advanced assays. The percentages of early/late apoptotic cells to which melatonin (IC50: 380 μM) was administered were increased …
The Effects Of Topical Melatonin On Oxidative Stress, Apoptosis Signals,And P53 Protein Expression During Cutaneous Wound Healing, Azi̇ze Şener, Özge Çevi̇k, Özge Doğan, Nazli Gül Altindi̇ş, Hali̇l Aksoy, Betül Okuyan
The Effects Of Topical Melatonin On Oxidative Stress, Apoptosis Signals,And P53 Protein Expression During Cutaneous Wound Healing, Azi̇ze Şener, Özge Çevi̇k, Özge Doğan, Nazli Gül Altindi̇ş, Hali̇l Aksoy, Betül Okuyan
Turkish Journal of Biology
Elimination of reactive oxygen species (ROS) can be an important strategy to improve healing of wounds. ROS have an effect on proliferation and cell survival signaling, which results in alteration of apoptotic pathways in cells. Melatonin has antioxidant properties on skin wounds. In our study, we investigated the effects of topical melatonin (3%, w/w) on apoptosis and p53 protein expression together with parameters of oxidative stress in a cutaneous excision wound model. Bcl-2 protein levels in wound tissue at the end of days 3, 7, and 14 were significantly increased, while caspase-3 activity and p53 protein expression in wound tissue …
Melatonin Attenuates Apoptosis And Mitochondrial Depolarization Levels In Hypoxic Conditions Of Sh-Sy5y Neuronal Cells Induced By Cobalt Chloride (Cocl2), Abdülhadi̇ Ci̇hangi̇r Uğuz, Ahmi̇ Öz, Büşra Yilmaz, Seda Altunbaş, Ömer Çeli̇k
Melatonin Attenuates Apoptosis And Mitochondrial Depolarization Levels In Hypoxic Conditions Of Sh-Sy5y Neuronal Cells Induced By Cobalt Chloride (Cocl2), Abdülhadi̇ Ci̇hangi̇r Uğuz, Ahmi̇ Öz, Büşra Yilmaz, Seda Altunbaş, Ömer Çeli̇k
Turkish Journal of Biology
Melatonin (MEL) and its metabolites serve as endogenous reactive oxygen species (ROS) scavengers and have a wide spectrum of antioxidant activity. Cobalt chloride is one of the commonly used hypoxia-mimetic agents, due to blocking the degradation of and triggering the accumulation of hypoxia-inducible factor-1 alpha (HIF-1α ) protein, which is very well known as a critical regulator of the cellular response against hypoxia. In the current study we aimed to determine the possible protective effects of melatonin on a cobalt chloride-induced hypoxia model of SH-SY5Y neuronal cells. Group I was the control group and SH-SY5Y cells were incubated in normal …
The Effects Of Agomelatine And Melatonin On Ecog Activity Of Absenceepilepsy Model In Wag/Rij Rats, Hati̇ce Aygün, Duygu Aydin, Sema İnanir, Fati̇h Eki̇ci̇, Mustafa Ayyildiz, Erdal Ağar
The Effects Of Agomelatine And Melatonin On Ecog Activity Of Absenceepilepsy Model In Wag/Rij Rats, Hati̇ce Aygün, Duygu Aydin, Sema İnanir, Fati̇h Eki̇ci̇, Mustafa Ayyildiz, Erdal Ağar
Turkish Journal of Biology
The aim of this study was to evaluate the effect of the melatonergic M1 and M2 receptor agonist and serotonergic 5-HT2C receptor antagonist agomelatine on the spike wave discharges (SWDs) seen in electrocorticographic (ECoG) recordings of WAG/Rij rats with absence epilepsy. Twenty-one WAG/Rij male rats were used in this study. Tripolar electrodes were placed on skulls and control ECoG activities were recorded. Experimental groups received normal saline (Group I: 1 mL, intraperitoneally (i.p)), agomelatine (Group II: 40 mg/kg, i.p), and melatonin (Group III: 40 mg/kg, i.p) injections for 7 days. Following this period, 2-h ECoG recordings were repeated. The number …
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Turkish Journal of Biology
Aluminum is the most abundant metal on earth, known for its toxicity to humans and laboratory animals. Melatonin, produced by the pineal gland, has been shown to be an effective antioxidant. In this study, we investigated the possible protective role of melatonin in modulating toxicity and oxidative stress induced by chronic exposure to aluminum in the small intestine of rats. Wistar albino rats were divided into five groups. Group I served as the control and received only physiological saline, Group II served as the control for the melatonin group and received ethanol and physiological saline, Group III received melatonin, Group …
Dna Protective And Antioxidative Effects Of Melatonin In Streptozotocin-Induced Diabetic Rats, Seli̇m Sekki̇n, Eda Duygu İpek, Murat Boyacioğlu, Cavi̇t Kum, Ümi̇t Karademi̇r, Hande Sultan Yalinkilinç, Mehmet Onur Ak, Hulki̇ Başaloğlu
Dna Protective And Antioxidative Effects Of Melatonin In Streptozotocin-Induced Diabetic Rats, Seli̇m Sekki̇n, Eda Duygu İpek, Murat Boyacioğlu, Cavi̇t Kum, Ümi̇t Karademi̇r, Hande Sultan Yalinkilinç, Mehmet Onur Ak, Hulki̇ Başaloğlu
Turkish Journal of Biology
Diabetes mellitus is a chronic disease characterized by elevated blood sugar levels. In diabetic patients, oxidative stress induced by the presence of excessive free radicals is closely associated with chronic in?ammation, leading to potential tissue damage. Melatonin (MEL) is a compound synthesized by the pineal gland and a scavenger of free radicals. The aim of this study was to research the effects of MEL on oxidative stress and its DNA protective effects in streptozotocin-induced diabetic rats. In total, 32 rats were equally divided into four experimental groups: control, melatonin, diabetic, and diabetic + melatonin. A single dose of streptozotocin (60 …
Effects Of Photoperiod Variations And Alpha-Lipoic Acid Treatment On Melatonin,Cortisol, And Oxidative Stress Levels In The Blood Of Rats, Cahi̇de Çevi̇k, Recep Aslan
Effects Of Photoperiod Variations And Alpha-Lipoic Acid Treatment On Melatonin,Cortisol, And Oxidative Stress Levels In The Blood Of Rats, Cahi̇de Çevi̇k, Recep Aslan
Turkish Journal of Biology
This study aimed to investigate the effects of frequently changing photoperiods and alpha-lipoic acid treatment on melatonin, cortisol, oxidative markers, and some blood parameters. Eighty-four rats were randomly and equally divided into 7 groups: a control group, 3 prolonged light groups, and 3 prolonged dark groups. The study was completed in 2 stages, with 1- and 4-week periods, respectively. Blood samples were obtained at the end of the first and fourth weeks, and at the end of the dark period for the first and second stages. Serum aspartate aminotransferase and creatinine levels were significantly elevated in the prolonged dark olive …
A New Entomopathogenic Nematode Species From Turkey, Steinernema Websteri (Rhabditida: Steinernematidae), And Its Virulence, Ci̇han Gökçe, Zeynep Erbaş, Hüseyi̇n Yilmaz, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
A New Entomopathogenic Nematode Species From Turkey, Steinernema Websteri (Rhabditida: Steinernematidae), And Its Virulence, Ci̇han Gökçe, Zeynep Erbaş, Hüseyi̇n Yilmaz, Zi̇hni̇ Demi̇rbağ, İsmai̇l Demi̇r
Turkish Journal of Biology
Entomopathogenic nematodes were isolated from a population of larvae of the cutworm Agrotis segetum Denis & Schiffermüller, 1775 (Lepidoptera: Noctuidae) collected from the Black Sea region of Turkey in 2009 using White traps. The isolated nematodes were identified as Steinernema websteri based on morphological and molecular properties. Steinernema websteri was also found for the first time in Turkey and in A. segetum larvae. The efficacy of isolates was tested on A. segetum larvae in plastic boxes. Different concentrations of nematodes (100, 300, and 500 infective juveniles (IJs) g^{-1} of dry sand) at 23 ± 1 °C were used. The obtained …
Genetic Integrity Assessment Of Cryopreserved Tomato (Lycopersicon Esculentum Mill.) Genotypes, Ana Coste, Dana Suteu, Ioan Bacila, Constantin Deliu, Sergiu Valimareanu, Adela Halmagyi
Genetic Integrity Assessment Of Cryopreserved Tomato (Lycopersicon Esculentum Mill.) Genotypes, Ana Coste, Dana Suteu, Ioan Bacila, Constantin Deliu, Sergiu Valimareanu, Adela Halmagyi
Turkish Journal of Biology
Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene $\beta$-cyclase gene (LCY-B) from leaves. The highest shoot regeneration after cryopreservation was 70% (Pontica) following 24 h osmoprotection in 0.5 M sucrose and 30 min dehydration in plant vitrification solution 2 (PVS2). The data inferred from AFLP showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues compared with the noncryopreserved controls. Although a single nucleotide polymorphism, a G→T …
Nkx3.1 Binding To Gpx2, Qscn6, Sod1, And Sod2 Promoters Contributes To Antioxidant Response Regulation Via Transactivation, Bi̇lge Debeleç Bütüner, Kemal Sami̇ Korkmaz
Nkx3.1 Binding To Gpx2, Qscn6, Sod1, And Sod2 Promoters Contributes To Antioxidant Response Regulation Via Transactivation, Bi̇lge Debeleç Bütüner, Kemal Sami̇ Korkmaz
Turkish Journal of Biology
NKX3.1 is a prostate-specific transcription factor that is regulated by the androgen receptor in the presence of androgens. It functions as a tumor suppressor against the development of prostatic intraepithelial neoplasia and primary prostate tumors. Here, a recognized approach combining in silico analysis and chromatin immunoprecipitation (ChIP) was used to identify the genes directly regulated by NKX3.1 promoter binding in LNCaP cells. Quantitative PCR using ChIP-captured DNAs as templates verified a subset of NKX3.1 binding motifs. Thus, in the presence of androgens, significant NKX3.1 binding occurs to promoters of GPX2, QSCN6, SOD1, and SOD2 genes that contribute to oxidative stress …
Identification Of An Aflp Marker Linked With Yellow Rust Resistance In Wheat (Triticum Aestivum L.), Handan Balta, Özge Karakaş Meti̇n, Funda Şentürk Akfirat, Fahri̇ye Ertuğrul, Semra Hasançebi̇, Yildiz Aydi̇n, Kadi̇r Akan, Zafer Mert, Müge Türet, Ahu Altinkut Uncuoğlu
Identification Of An Aflp Marker Linked With Yellow Rust Resistance In Wheat (Triticum Aestivum L.), Handan Balta, Özge Karakaş Meti̇n, Funda Şentürk Akfirat, Fahri̇ye Ertuğrul, Semra Hasançebi̇, Yildiz Aydi̇n, Kadi̇r Akan, Zafer Mert, Müge Türet, Ahu Altinkut Uncuoğlu
Turkish Journal of Biology
An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F_2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP …
Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin
Prothoracicotropic Hormone-Producing Neurosecretory Neurons And Antioxidative Defense In Midgut Of Lymantria Dispar In Trophic Stress, Vesna Peric Mataruga, Milena Vlahovic, Marija Mrdakovic, Dajana Todorovic, Dragana Matic, Anja Gavrilovic, Larisa Ilijin
Turkish Journal of Biology
As a very invasive insect species, Lymantria dispar is adaptable and sensitive to a changing environment. In insects the neuroendocrine system first reacts to stress by production of prothoracicotropic neurohormones (PTTH) that control ecdysteroid synthesis (morphogenetic and stress hormones). In this article, we report changes in the L2' brain neurosecretory neurons that synthesize PTTH in L. dispar larvae after feeding on locust tree leaves (Robinia pseudoacacia), an unsuitable host plant. Groups of larvae (n = 20 per experimental group) were offered this in comparison with oak leaves (Quercus robur), a suitable control diet, for 3 days after molting into the …
Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi
Optimization Of E. Coli Culture Conditions For Efficient Dna Uptake By Electroporation, Irfan Ahmad, Tehseen Rubbab, Farah Deeba, Syed Muhammad Saqlan Naqvi
Turkish Journal of Biology
Bacterial transformation is a significant breakthrough in the field of molecular biology for cloning purposes. A variety of chemical and physical methods are utilized for enhancing bacterial competence. The present study was designed, via the consolidation of chemical and physical methods, to improve competence of cells for high transformation efficiency up to 10^9 transformants/µg of DNA. LB and SOC media and their supplemented combinations with osmotic agents (sucrose and glycine) were used to prepare electrocompetent cells of E. coli DH5\alpha at temperatures of 25 and 37 °C, which were then subjected to electroporation. The results showed that cells grown in …
Phylogenetic Analysis And Characterization Of An Alkane-Degrading Yeast Strain Isolated From Oil-Polluted Soil, Ortansa Csutak, Tatiana Vassu
Phylogenetic Analysis And Characterization Of An Alkane-Degrading Yeast Strain Isolated From Oil-Polluted Soil, Ortansa Csutak, Tatiana Vassu
Turkish Journal of Biology
The isolation and phylogenetic analysis of new yeast strains represent an important basis for the development of practical applications in biotechnology, industry, and bioremediation. The yeast strain RG1 was isolated from oil-polluted soil and characterized using morphological observations and the API 20C AUX system. The amplified ITS1-5.8S rDNA-ITS2 region had 653 bp and the restriction profiles obtained with Cfo I, Dde I, Hae III, Hinf I, and Hpa II endonucleases showed high similarities with those from Rhodotorula glutinis reference strains. The phylogenetic tree based on the Cfo I patterns confirmed the classification of the strain RG1 as belonging to the …
Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi
Cloning, Purification, And Characterization Of A Thermophilic Ribulokinase From Anoxybacillus Kestanbolensis Ac26sari, Müslüm Tokgöz, Kadri̇ye İnan, Ali̇ Osman Beldüz, Öznur Gedi̇kli̇, Sabri̇ye Çanakçi
Turkish Journal of Biology
The gene encoding ribulokinase araB from Anoxybacillus kestanbolensis AC26Sari was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21 under the control of isopropyl-\beta-D-thiogalactopyranoside-inducible T7 promoter. The enzyme, designated as AC26RK, was purified with the MagneHis Protein Purification System. The molecular mass of the native protein, as determined by SDS-PAGE, was about 61 kDa. AC26RK was active throughout a broad pH (pH 5.0-10.0) and temperature (50-75 °C) range, and it had an optimum pH of 9.0 and optimum temperature of 60 °C. The enzyme displayed about 90%-100% of its original activities after a 30-min incubation at a …
Progesterone Receptor Modulators In Breast Cancer, Ronald D. Wiehle
Progesterone Receptor Modulators In Breast Cancer, Ronald D. Wiehle
Turkish Journal of Biology
Breast cancer has been treated successfully with selective estrogen receptor antagonists (SERMs) such as tamoxifen, receptor-depleting agents such as fulvestrant, and aromatase inhibitors such as anastrozole. Selective progesterone receptor modulators (SPRMs or PRMs) have not been studied as much and are currently under investigation for inhibition of mammary carcinogenesis in animal models and breast cancer prevention trials in women. They might follow tamoxifen and aromatase inhibitors in the adjuvant treatment of breast cancers with acquired resistance. These uses have not provoked ground-breaking progress or studies and PRMs do not have a high profile. Most in vitro and in vivo studies …
Loss Of Heterozygosity In Ing3 And Ing5 Genes In Breast Cancer, Esra Gündüz, Gökhan Nas, Muradi̇ye Acar, Eyyüp Üçtepe, Mi̇kdat Bozer, Murat Öznur, Reyhan Bayrak, Mehmet Gündüz
Loss Of Heterozygosity In Ing3 And Ing5 Genes In Breast Cancer, Esra Gündüz, Gökhan Nas, Muradi̇ye Acar, Eyyüp Üçtepe, Mi̇kdat Bozer, Murat Öznur, Reyhan Bayrak, Mehmet Gündüz
Turkish Journal of Biology
The tumor suppressor genes (TSGs) ING3 and ING5, members of the inhibitor of growth gene family, are effective in inhibition of cell growth and induction of apoptosis. However, in many cancer types, one of the alleles of a TSG is lost through carcinogenesis, while the remaining allele is usually inactivated through a process called loss of heterozygosity (LOH). Previous studies in head and neck cancer revealed that allelic loss and reduced expression is a common pattern of ING gene family members. Fifty paraffin-embedded breast cancer tissues were analyzed by polymerase chain reaction and denatured-polyacrylamide gel electrophoresis for LOH status. The …