Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Apoptosis (44)
- Antimicrobial activity (32)
- Cancer (32)
- Oxidative stress (31)
- Cytotoxicity (30)
-
- Antioxidant (25)
- COVID-19 (25)
- Breast cancer (24)
- SARS-CoV-2 (24)
- Melatonin (23)
- Antioxidant activity (22)
- Purification (20)
- Mesenchymal stem cells (19)
- Turkey (18)
- Antioxidant enzymes (17)
- Rat (17)
- Antibacterial activity (15)
- Inflammation (15)
- Gene expression (14)
- Regeneration (14)
- Bioinformatics (13)
- Characterization (13)
- Liver (13)
- Antibacterial (12)
- Genetic diversity (12)
- Growth (12)
- Saccharomyces cerevisiae (11)
- Streptomyces (11)
- Toxicity (11)
- Catalase (10)
- Publication Year
Articles 481 - 510 of 1681
Full-Text Articles in Biology
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
In Vitro Evaluation Of Plla/Pbs Sponges As A Promisingbiodegradable Scaffold For Neural Tissue Engineering, İrem Ayşe Kanneci̇ Altinişik, Fatma Neşe Kök, Deni̇z Yücel, Gamze Köse
Turkish Journal of Biology
In tissue engineering, the use of poly-L-lactic acid (PLLA)/polybutylene succinate (PBS) blend for the construction of scaffold is very limited. Moreover, polymeric sponges fabricated from PLLA/PBS have not been studied for neural tissue engineering. In the present study, the potential of the utility of PLLA/PBS polymeric sponges seeded with Schwann cells was investigated. PLLA and PBS were blended in order to increase the processability and tune the crystallinity, porosity, and degradation rate of the resulted polymeric sponges. These sponges were then seeded with Schwann cells. Porosity analysis showed that there were no significant differences between different compositions of PLLA/PBS blends; …
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Accumulation Of Phenolics In Natural And Micropropagated Plantlets Of Thymus Pseudopulegioides Klokov & Des.-Shost. With Their Antioxidant Potentials, Mustafa Günaydin, Abdul Hafeez Laghari, Ersan Bektaş, Münevver Sökmen, Atalay Sökmen
Turkish Journal of Biology
Thymus pseudopulegioides plantlets were propagated in vitro via direct organogenesis by using Murashige and Skoog (MS) media containing kinetin, thidiazuron, and 6-benzyladenine (BA) individually. Methanol extracts obtained both from plantlets and wild plants were analyzed for their total phenolics and flavonoid contents, then quantified by HPLC. The highest total phenolic (8.83 mg/g as gallic acid equivalent) and total flavonoid (0.92 mg/mL as rutin equivalent) values were from the MS media supplemented with 1.0 mg/L kinetin and 0.5 mg/L BA, respectively. The plantlets grown in those media also showed remarkable antioxidant activities with an IC50 value of 4.77 \mug/mL in DPPH …
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Proteomics Analysis Of Mitochondrial Dysfunction Triggered By Complex Specific Electron Transport Chain Inhibitors Reveals Common Pathways Involving Protein Misfolding In An Sh-Sy5y In Vitro Cell Model, Betül Şahi̇n, Ahmet Tarik Baykal
Turkish Journal of Biology
Mitochondrial dysfunction has been previously identified in neurodegenerative diseases such as Alzheimer disease, Huntington disease, and Parkinson disease. Chemical inhibition of the mitochondrial electron transport chain (ETC) was shown to trigger symptoms in animal models similar to those observed in human neurodegenerative diseases. In order to understand the effect of mitochondrial dysfunction on the proteome level, LC-MSE-based bottom-up, label-free differential proteomics expression analysis was used to monitor protein level changes in SH-SY5Y neuroblastoma cells induced by ETC-specific inhibitors (MPTP, 3-NP, sodium azide, antimycin A, and oligomycin). A total of 379 proteins were identified across the sample set and 75 of …
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Turkish Journal of Biology
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
Turkish Journal of Biology
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and …
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Turkish Journal of Biology
The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth …
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Effects Of Celecoxib And L-Name On Apoptosis And Cell Cycle Ofmcf-7 Cd44+/Cd24-/Low Subpopulation, Maryam Majdzadeh, Shima Aliebrahimi, Melody Vatankhah, Seyed Nasser Ostad
Turkish Journal of Biology
Recent studies have reported that cancer stem cells (CSCs) play a pivotal role in treatment failure, causing cancer recurrence. Here, we investigated the effects of L-NAME (an iNOS inhibitor) and celecoxib (a selective COX-2 inhibitor) on CSC-like cells (CSC-LCs) and their parental cells. Breast CSC-LCs derived from the MCF-7 cell line were sorted and characterized with the CD44+/CD24-/low phenotype. After isolation, the percentage of the subpopulation expressing CD44+/CD24-/low biomarkers increased considerably from 0.96% to 28.6%. Use of L-NAME and celecoxib showed antiproliferative activity towards both MCF-7 and CSC-LCs. Although celecoxib enhanced apoptotic cell death, the CSC-LC population was more resistant …
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Effect Of Mutation In Active Site Residue Trp209 To Val, Leu, Ile And Pro On The Catalytic Activity And Affinity For Some Benzenesulfonamides Of Human Carbonic Anhydrase Ii, Deryanur Kiliç, Orhan Erdoğan, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Biology
Human carbonic anhydrase II (hCA II) enzyme was firstly expressed using a pET-SUMO expression vector in Escherichia coli and the recombinant enzyme was purified using nickel (Ni2+) affinity chromatography. The substitutions of Trp 209 with four amino acid (Val, Leu, Ile, and Pro) in the hydrophobic pocket of hCA II were conducted using site-directed mutagenesis. The p-nitrophenyl esterase activity of hCA II variants correlates with the hydrophobicity and size of residue, suggesting that the hydrophobic character of this residue is important for catalysis. The Trp 209 was forecast as an important residue and was exposed to computational mutagenesis. This forecast …
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Extracellular Presence/Release Of Galectins From Htr-8/Svneo Extravillous Trophoblast Cells*, Danica Cujic, Maja Kosanovic, Milica Jovanovic Krivokuca, Ljiljana Vicovac, Miroslava Jankovic
Turkish Journal of Biology
Galectins (gals) are ß-galactoside binding lectins, involved in many processes at the fetomaternal interface where they can exert their roles both in and out of cells. The aim of this work was to explore the extracellular presence/release of HTR-8/SVneo extravillous trophoblast cell line galectins. To that end, conditioned medium (CM) from HTR-8/SVneo cell culture was fractionated into a high molecular mass fraction (HMF) and low molecular mass fraction (LMF) using 100 kDa cut-off concentrators. In addition, extracellular vesicles (EVs) were isolated from CM by ultracentrifugation. Size and shape of the EVs were analyzed by transmission electron microscopy and their galectin …
Effect Of Calprotectin Subunit S100a9 On The Expression And Methylation Of Ocln In Human Melanoma Cell Line A-375, Mostafa Najafi, Safar Ali Alizadeh, Mehdi Azad, Taghi Naserpour Farivar, Farzad Rajaei, Kourosh Hotam Sorouri, Babak Rahmani, Nematollah Gheibi
Effect Of Calprotectin Subunit S100a9 On The Expression And Methylation Of Ocln In Human Melanoma Cell Line A-375, Mostafa Najafi, Safar Ali Alizadeh, Mehdi Azad, Taghi Naserpour Farivar, Farzad Rajaei, Kourosh Hotam Sorouri, Babak Rahmani, Nematollah Gheibi
Turkish Journal of Biology
Increased levels of calprotectin subunits S100A8 and S100A9 have been detected in human cancers. Melanoma is the most aggressive type of skin cancer, and its treatment is challenging because of its brain metastasis. OCLN encodes occludin, which plays a major role in the formation and regulation of tight junctions. The aim of this study was to evaluate the methylation status of the OCLN promoter and its expression in A-375 melanoma cells treated with or without various concentrations of S100A9 for 24, 48, and 72 h. Total RNA was extracted, and synthesized cDNA was assessed by performing real-time PCR. MSP-PCR was …
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Turkish Journal of Biology
The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. Cotton plants were grown with different concentrations of MgSO4 in hydroponic medium to create nutrient deficiency (0, 75, 150, 1000 μM Mg). Gradual decreases in growth and photosynthetic rates were observed with declining Mg concentrations and 0, 75, and …
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Turkish Journal of Biology
Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 …
Screening Of Yeast Isolates From Flowers For Effective Ethanol Production, Asma Chaudhary, Shuichi Karita
Screening Of Yeast Isolates From Flowers For Effective Ethanol Production, Asma Chaudhary, Shuichi Karita
Turkish Journal of Biology
The use of alternative substrates to produce biofuel is a striking option nowadays. This study aimed to screen bioethanol-producing yeast strains. From different flowers, 65 yeasts were isolated. Twelve isolates assimilated glucose by liberation of CO2 anaerobically. Out of these, only 5 yeast isolates fermented glucose in medium consisting of 0.8 g/L Mg2+ ions to produce 2.05 $\pm$ 0.03% ethanol. The selected five strains were identified as members of the genera Metschnikowia or Meyerozyma based on molecular characterization. Selected yeast strains were used for conversion of rice into bioethanol following dilute acid hydrolysis and fermentation. Consistent ethanol production was 1.80 …
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Determining Expression Of Mirnas That Potentially Regulatestat5a And 5b In Dasatinib-Sensitive K562 Cells, Asu Fergün Yilmaz, Burçi̇n Kaymaz, Çağdaş Aktan, Nur Soyer, Buket Kosova, Ajda Güneş, Fahri̇ Şahi̇n, Melda Özkan, Güray Saydam, Fi̇li̇z Vural
Turkish Journal of Biology
In the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ΔΔCT method. At the last stage of the study, we …
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Mesoporous Silica Nanoparticles Sba-15 Loaded With Emodin Upregulate The Antioxidative Defense Of Euproctis Chrysorrhoea (L.) Larvae, Milena Jankovic-Tomanic, Dajana Todorovic, Zoran Stanivukovic, Vesna Peric Mataruga, Ludger Wessjohann, Goran Kaludjerovic
Turkish Journal of Biology
The study presented here aims to elucidate the effects of emodin (EO = 1,3,8-trihydroxy-6-methylanthraquinone) in its free form and when loaded into a mesoporous silica nanocarrier SBA-15 (→ SBA-15 EO) on the activities of the main antioxidative enzymes, superoxide dismutase, catalase, glutathione S-transferase, and glutathione reductase, in larvae of a polyphagous insect pest, the browntail moth Euproctis chrysorrhoea (L.). The results show that only SBA-15 EO upregulates the activities of the tested antioxidative enzymes. These results point to significant differences in the effectiveness of the compound in the free versus the loaded form.
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Turkish Journal of Biology
Lead (Pb) has wide-ranging effects on various essential physiological processes in plants including seed germination, root/shoot growth, photosynthetic efficiency, water status, and activities of enzymes. The effect of combined treatment of 24-epibrassinolide (10-7 M, EBL) and salicylic acid (1 mM, SA) on growth, photosynthetic attributes, and phenolic compounds in 30-, 60-, and 90-day-old plants of Brassica juncea L. under Pb stress (0.25 mM, 0.50 mM, and 0.75 mM) were studied. It was observed that Pb toxicity resulted in lowered growth and photosynthetic efficiency. The expressions of CHLASE, PSY, CHS, and PAL genes were altered. Presoaking treatment with the combination of …
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Optimizations Needed For Lateral Flow Assay For Rapid Detection Of Pathogenic E. Coli, Di̇lek Çam, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Lateral flow assay (LFA), or the immunochromatographic strip test, is popular to use for rapid and sensitive immunoassays. Gold nanoparticles (GNPs), due to tunable optical characteristics and easy manipulation of size or shape, represent an attractive approach for LFA technology. Since most enterohemorrhagic infections result from water and food contaminations of Escherichia coli O157:H7, selective and rapid detection of this organism in environmental and biological complexes is necessary. In this study, optimized parameters of antibody (Ab)-based LFA for rapid detection of pathogenic E. coli O157:H7 are described. GNPs were used as visualizing agents. The measuring parameters include the Ab concentration …
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Gelatin Positively Regulates The Immunosuppressive Capabilitiesof Adipose-Derived Mesenchymal Stem Cells, Imdad Ullah Khan, Yongseok Yoon, Wan Hee Kim, Oh-Kyeong Kweon
Turkish Journal of Biology
The characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. The expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 …
Efficient Polyhydroxybutyrate Production From Bacillus Thuringiensis Using Sugarcane Juice Substrate, Anon Thammasittirong, Sudarat Saechow, Sutticha Na-Ranong Thammasittirong
Efficient Polyhydroxybutyrate Production From Bacillus Thuringiensis Using Sugarcane Juice Substrate, Anon Thammasittirong, Sudarat Saechow, Sutticha Na-Ranong Thammasittirong
Turkish Journal of Biology
The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. Among 602 local Bacillus isolates, Bacillus thuringiensis B417-5 produced the highest amount of PHB (2.278 g/L, 60.07% of dry cell weight, DCW). 1H NMR and FTIR analyses of the extracted polymer revealed the characteristic peaks of PHB. The optimization results showed that the highest PHB accumulation (2.768 g/L, 72.08% of DCW) was achieved when culturing B. thuringiensis B417-5 in a nitrogen-deficient medium containing 1% total sugar from sugarcane juice and 0.5% yeast extract, with a pH of 7.0 and an …
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Improved Insulin-Secreting Properties Of Pancreatic Islet Mesenchymalstem Cells By Constitutive Expression Of Pax4 And Mafa, Ayşegül Açiksari, Gökhan Duruksu, Erdal Karaöz
Turkish Journal of Biology
For long-term treatment of diabetes type 1, transplantation of insulin-producing beta cells may be a promising method, but the limited number of islets for transplantation requires the development of different approaches. In this study, we aimed to generate beta-like insulin-producing cells. For this purpose, MafA, Pax4, and Ngn3 genes were transferred into pancreatic islet-derived mesenchymal stem cells, and the effect of their ectopic expressions on differentiation efficiency was examined. Stemness properties of pancreatic islet stem cells were characterized. The 3 genes were transfected by electroporation and expressed constitutively. The transfected cells were further stimulated to differentiate by using chemical induction. …
Overexpression Of The Ibor Gene From Sweet Potato (Ipomea Batatas `Hoang Long') In Maize Increases Total Carotenoid And Ss-Carotene Contents, Thi Luong Tran, Thi Huong Ho, Duc Thanh Nguyen
Overexpression Of The Ibor Gene From Sweet Potato (Ipomea Batatas `Hoang Long') In Maize Increases Total Carotenoid And Ss-Carotene Contents, Thi Luong Tran, Thi Huong Ho, Duc Thanh Nguyen
Turkish Journal of Biology
Nutritional quality of most maize varieties is very low due to the lack of lysine and tryptophan and extremely low provitamin A carotenoids including ß-carotene, Α-carotene, and ß-cryptoxanthin. In this study, we report the successful overexpression of the IbOr gene in H145 and H95 inbred maize lines under the control of maize seed-specific promoter globulin 1 (Glo1) for the purpose of improving ß-carotene in maize. The results showed that the total carotenoid and ß-carotene content of all analyzed transgenic maize plants were significantly higher than those of wild-type lines. For H145-IbOr transgenic maize, in the best line (H145-IbOr.10), the total …
Identification Of Proteins Differentially Accumulated In Enterococcus Faecalis Under Acrylamide Exposure, Aleksandra Bocian, Konrad Hus, Marcin Jaromin, Miroslaw Tyrka, Andrzej Lyskowski
Identification Of Proteins Differentially Accumulated In Enterococcus Faecalis Under Acrylamide Exposure, Aleksandra Bocian, Konrad Hus, Marcin Jaromin, Miroslaw Tyrka, Andrzej Lyskowski
Turkish Journal of Biology
Analysis of bacterial proteomes can be used to obtain large amounts of information about adaptive microbial mechanisms to changing extracellular conditions. In the past, many bacterial species with the ability to degrade acrylamide were isolated. In this study differences in the Enterococcus faecalis proteome upon acrylamide exposure were investigated. We revealed substantial changes in the proteome of bacteria cultured in different environmental conditions. Microorganisms exposed to acrylamide showed higher accumulation of proteins associated with energy metabolism and its regulation. Moreover, several proteins involved in protection of cells from stress conditions were also identified. These biomacromolecules are involved in proper folding …
Converted Carotenoid Production In Dunaliella Salina By Using Cyclization Inhibitors 2-Methylimidazole And 3-Amino-1,2,4-Triazole, Arzu Yildirim, İsmai̇l Hakki Akgün, Meltem Dalay
Converted Carotenoid Production In Dunaliella Salina By Using Cyclization Inhibitors 2-Methylimidazole And 3-Amino-1,2,4-Triazole, Arzu Yildirim, İsmai̇l Hakki Akgün, Meltem Dalay
Turkish Journal of Biology
Carotenoids are vital for most photosynthetic organisms because of their crucial role in prevention of the damage caused by excess light or stress conditions like heat or nutrient deprivation. Some of them are also valuable for biotechnology wıth respect to their colorful feature and antioxidant properties. In this study, a natural β-carotene producer, green microalga Dunaliella salina, was evaluated for its potential to produce different valuable carotenoids through the inhibition of cyclization reactions in the carotenoid pathway by the chemical inhibitors 2-methylimidazole (2MI) and 3-amino-1,2,4-triazole (Amitrol). 2MI was shown to be effective on lutein accumulation in D. salina cultures grown …
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Turkish Journal of Biology
This article explores the relationship between the renal expression of cystathionine gamma lyase (CSE) and endothelial nitric oxide synthase (eNOS) and the responsiveness of α1A adrenergic receptors in the renal vasculature following left ventricular hypertrophy (LVH) in rats. LVH was established by administering isoprenaline (5 mg/kg, 5 injections subcutaneously, 72 h apart) with 62 mg/L caffeine in drinking water for 2 weeks. Renal vasoconstrictor responses were measured using local administration of adrenergic agonists noradrenaline (NA), phenylephrine (PE), and methoxamine (ME) and the selective α1A adrenergic antagonist 5-methylurapidil (5-MeU). Mean arterial blood pressure was higher (144 ± 9 vs. 116 ± …
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Turkish Journal of Biology
Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production. It has the capacity to tolerate marginal growing conditions better than most other crops. In this study, a proline- and glycine-rich protein encoding gene, IbPGAHRP1, was isolated and characterized from the transcriptome of sweet potato cultivar Xushu 18. Sequence analyses showed that proline, glycine, alanine, and histidine account for 62.6% of the total amino acid residues. Two proline-rich tandem repeats, an alanine-rich repeat, a histidine-rich tandem repeat, and five XYPP motifs were present in IbPGAHRP1. Sequence similarity analysis …
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Turkish Journal of Biology
Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into …
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Inorganic phosphate is stored as a polyphosphate (polyP) polymer inside every living cell. This polymer is synthesized by the polyP kinase (PPK) enzyme using the terminal phosphate of ATP as substrate and it performs important functions in the cell. In this study, effects of high levels of PPK on Bacillus thuringiensis subsp. israelensis were analyzed. Recombinant Bti ppk, a PPK overproducer, was found to uptake more phosphate into the cell and produce a consistently higher amount of endotoxin than the wild type under culture conditions including a range of temperatures (25 °C, 30 °C, and 37 °C), pH values (pH …
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Turkish Journal of Biology
Neoplastic cells need essential metals, such as iron and copper, for cellular functions and rapid growth. Metal chelation and changes in their redox cycle in favor of oxidative stress may be critical for making these cells vulnerable to cell death. The HER2-overexpressing breast cancer cell line, MCF7-HER2, and its vehicle control, MCF7-vec cells, were treated with CuCl2, FeSO4, and Dp44mT. Reactive oxygen species (ROS) production in response to these chemicals was detected with flow cytometry, and cell viability was measured by MTT assay in the cells. ROS levels were relatively increased by Dp44mT in the cells, and this was reversed …
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Turkish Journal of Biology
The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools. The study resulted in identifying antigenic regions in the beginning and middle (3-17 and 965-997 amino acid residues) as suitable binders to MHCII and the carboxyl terminal of the protein (1800-1958 amino acid residues) in B-cell epitopes. The appropriate region in B-cell epitopes was chosen for cloning. The presence of recombinant protein was detected with immunological methods. Subsequently, BALB/C mice were immunized with …
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Turkish Journal of Biology
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the …