Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Apoptosis (44)
- Antimicrobial activity (32)
- Cancer (32)
- Oxidative stress (31)
- Cytotoxicity (30)
-
- Antioxidant (25)
- COVID-19 (25)
- Breast cancer (24)
- SARS-CoV-2 (24)
- Melatonin (23)
- Antioxidant activity (22)
- Purification (20)
- Mesenchymal stem cells (19)
- Turkey (18)
- Antioxidant enzymes (17)
- Rat (17)
- Antibacterial activity (15)
- Inflammation (15)
- Gene expression (14)
- Regeneration (14)
- Bioinformatics (13)
- Characterization (13)
- Liver (13)
- Antibacterial (12)
- Genetic diversity (12)
- Growth (12)
- Saccharomyces cerevisiae (11)
- Streptomyces (11)
- Toxicity (11)
- Catalase (10)
- Publication Year
Articles 241 - 270 of 1681
Full-Text Articles in Biology
Detection Of Grain Yield Qtls In The Durum Population Lahn/Cham1 Tested Incontrasting Environments, Issame Farouk, Ahmad Alsaleh, Jihan Motowaj, Fatima Gaboun, Bouchra Belkadi, Abdelkarim Filali Maltouf, Zakaria Kehel, Ismahane Elouafi, Nasserelhaq Nsarellah, M. Miloudi Nachit
Detection Of Grain Yield Qtls In The Durum Population Lahn/Cham1 Tested Incontrasting Environments, Issame Farouk, Ahmad Alsaleh, Jihan Motowaj, Fatima Gaboun, Bouchra Belkadi, Abdelkarim Filali Maltouf, Zakaria Kehel, Ismahane Elouafi, Nasserelhaq Nsarellah, M. Miloudi Nachit
Turkish Journal of Biology
Durum wheat (Triticum turgidum L. var durum) is tetraploid wheat (AABB); it is the main source of semolina and other pasta products. Grain yield in wheat is quantitatively inherited and influenced by the environment. The genetic map construction constitutes the essential step in identifying quantitative trait loci (QTLs) linked to complex traits, such as grain yield. The study aimed to construct a genetic linkage map of two parents that are widely grown durum cultivars (Lahn and Cham1) in the Mediterranean basin, which is characterized by varying climate changes. The genetic linkage map of Lahn/Cham1 population consisted of 112 recombinant inbred …
Expression Profiles Of Interferon-Related Genes In Cells Infected With Influenza A Viruses Or Transiently Transfected With Plasmids Encoding Viral Rna Polymerase, Eli̇f Çağlayan, Kadi̇r Turan
Expression Profiles Of Interferon-Related Genes In Cells Infected With Influenza A Viruses Or Transiently Transfected With Plasmids Encoding Viral Rna Polymerase, Eli̇f Çağlayan, Kadi̇r Turan
Turkish Journal of Biology
Influenza A viruses frequently change their genetic characteristics, which leads to the emergence of new viruses. Consequently, elucidation of the relationship between influenza A virus and host cells has a great importance to cope with viral infections. In this study, it was aimed to determine expression profiles of interferon response genes in human embryonic kidney 293 (HEK293) cells infected with human (A/WSN-H1N1) and avian influenza A viruses (duck/Pennsylvania/10218/84/H5N2) or transfected with plasmids encoding viral RdRP subunits and, to obtain clues about the genes that may be important for the viral pathogenesis. The HEK293 cells cultured in a 12-well plate were …
Current Mutatome Of Sars-Cov-2 In Turkey Reveals Mutations Of Interest, Doğa Eski̇er, Evren Akalp, Özlem Dalan, Gökhan Karakülah, Yavuz Oktay
Current Mutatome Of Sars-Cov-2 In Turkey Reveals Mutations Of Interest, Doğa Eski̇er, Evren Akalp, Özlem Dalan, Gökhan Karakülah, Yavuz Oktay
Turkish Journal of Biology
As the underlying pathogen for the COVID-19 pandemic that has affected tens of millions of lives worldwide, SARS-CoV-2 and its mutations are among the most urgent research topics worldwide. Mutations in the virus genome can complicate attempts at accurate testing or developing a working treatment for the disease. Furthermore, because the virus uses its own proteins to replicate its genome, rather than host proteins, mutations in the replication proteins can have cascading effects on the mutation load of the virus genome. Due to the global, rapidly developing nature of the COVID-19 pandemic, local demographics of the virus can be difficult …
Targeting The Tumor Stroma: Integrative Analysis Reveal Gata2 And Toryaip1 As Novel Prognostic Targets In Breast And Ovarian Cancer, Ömer Faruk Erceylan, Ayşe Savaş, Esra Göv
Targeting The Tumor Stroma: Integrative Analysis Reveal Gata2 And Toryaip1 As Novel Prognostic Targets In Breast And Ovarian Cancer, Ömer Faruk Erceylan, Ayşe Savaş, Esra Göv
Turkish Journal of Biology
Tumor stroma interaction is known to take a crucial role in cancer growth and progression. In the present study, it was performed gene expression analysis of stroma samples with ovarian and breast cancer through an integrative analysis framework to identify common critical biomolecules at multiomics levels. Gene expression datasets were statistically analyzed to identify common differentially expressed genes (DEGs) by comparing tumor stroma and normal stroma samples. The integrative analyses of DEGs indicated that there were 59 common core genes, which might be feasible to be potential marks for cancer stroma targeted strategies. Reporter molecules (i.e. receptor, transcription factors and …
Prediction Of Host-Pathogen Protein Interactions By Extended Network Model, İrfan Kösesoy, Murat Gök, Tamer Kahveci̇
Prediction Of Host-Pathogen Protein Interactions By Extended Network Model, İrfan Kösesoy, Murat Gök, Tamer Kahveci̇
Turkish Journal of Biology
Knowledge of the pathogen-host interactions between the species is essentialin order to develop a solution strategy against infectious diseases. In vitro methods take extended periods of time to detect interactions and provide very few of the possible interaction pairs. Hence, modelling interactions between proteins has necessitated the development of computational methods. The main scope of this paper is integrating the known protein interactions between thehost and pathogen organisms to improve the prediction success rate of unknown pathogen-host interactions. Thus, the truepositive rate of the predictions was expected to increase.In order to perform this study extensively, encoding methods and learning algorithms …
Transcriptome Profiles Associated With Selenium-Deficiency-Dependent Oxidative Stress Identify Potential Diagnostic And Therapeutic Targets In Liver Cancer Cells, Damla Gözen, Deni̇z Cansen Kahraman, Kübra Narci, Huma Shehwana, Özlen Konu, Rengül Çeti̇n Atalay
Transcriptome Profiles Associated With Selenium-Deficiency-Dependent Oxidative Stress Identify Potential Diagnostic And Therapeutic Targets In Liver Cancer Cells, Damla Gözen, Deni̇z Cansen Kahraman, Kübra Narci, Huma Shehwana, Özlen Konu, Rengül Çeti̇n Atalay
Turkish Journal of Biology
Hepatocellular carcinoma (HCC) is one of the most common cancer types with high mortality rates and displays increased resistance to various stress conditions such as oxidative stress. Conventional therapies have low efficacies due to resistance and off-target effects in HCC. Here we aimed to analyze oxidative stress-related gene expression profiles of HCC cells and identify genes that could be crucial for novel diagnostic and therapeutic strategies. To identify important genes that cause resistance to reactive oxygen species (ROS), a model of oxidative stress upon selenium (Se) deficiency was utilized. The results of transcriptome-wide gene expression data were analyzed in which …
Arid4b Physically Interacts With Tfap2c In Mouse Embryonic Stem Cells, Ezgi̇ Gül Keski̇n, Jialiang Huang, Ni̇hal Terzi̇ Çi̇zmeci̇oğlu
Arid4b Physically Interacts With Tfap2c In Mouse Embryonic Stem Cells, Ezgi̇ Gül Keski̇n, Jialiang Huang, Ni̇hal Terzi̇ Çi̇zmeci̇oğlu
Turkish Journal of Biology
Precise regulation of gene expression is required for embryonic stem cell (ESC) differentiation. Transcription factor (TF) networks coordinate the balance of pluripotency and differentiation in response to extracellular and intracellular signals. Chromatin factors work alongside TFs to achieve timely regulation of gene expression for differentiation process. Our previous studies showed that a member of the Sin3a corepressor complex, Arid4b, is critical for proper mouse ESC differentiation into mesoderm and endoderm. We found elevated histone 3 lysine 27 acetylation (H3K27Ac) in a subset of genomic loci in meso/endoderm directed arid4b? cells, coincident with their derepression. We reasoned that Sin3a complex may …
Positive Regulation Of Tnfr1 Signaling Via Sh3 Recognition Motif, Fatma Ece Çopuroğlu, Fatma Zehra Hapi̇l Zevkli̇ler, Şükran Burçak Yoldaş, Osman Ni̇dai̇ Özeş
Positive Regulation Of Tnfr1 Signaling Via Sh3 Recognition Motif, Fatma Ece Çopuroğlu, Fatma Zehra Hapi̇l Zevkli̇ler, Şükran Burçak Yoldaş, Osman Ni̇dai̇ Özeş
Turkish Journal of Biology
TNF is a pleiotropic cytokine and shows its biological function by binding to its receptors called TNFR1 and TNFR2. While TNFR1 induces apoptosis by activation of caspase-8 via the "death domain", it also activates IKKα/β, MKK3/6, MKK4/7 by activation of TAK1. Although the TNFR1 signaling pathway is known by in large, it is not known how AKT and MAPKs p38, ERK1/2, and JNK1/2 are activated. The presence of a proline-rich PPAP region, (P448PAP451, a binding site for the SH3 domain-containing proteins) very close to the C-terminus promoted us to determine whether this region has any role in the TNFR1 signal …
Negative Regulation Of Diminutive Cancer Regulator Through Differentiation And Microrna Pathway Components In Drosophila Cells, Sumira Malik
Negative Regulation Of Diminutive Cancer Regulator Through Differentiation And Microrna Pathway Components In Drosophila Cells, Sumira Malik
Turkish Journal of Biology
Drosophila model is intensively studied for the development of cancer. The diminutive (dMyc), a homolog of the human MYC gene, is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells. Here we report protein complex of Argonaute 1 (AGO1), Bag of marbles (Bam), and Brain tumor (Brat) proteins and not the individual factor of this complex repression of …
Compensatory Expression Regulation Of Highly Homologous Proteins Hnrnpa1 Andhnrnpa2, Yan Chang, Xiao Feng Lu, Jia Ying Qiu
Compensatory Expression Regulation Of Highly Homologous Proteins Hnrnpa1 Andhnrnpa2, Yan Chang, Xiao Feng Lu, Jia Ying Qiu
Turkish Journal of Biology
Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains. Transfection of HEK293 cells with small interfering RNA and overexpression vectors of HNRNPA1 and HNRNPA2 demonstrated that HNRNPA1 and HNRNPA2 paralogs regulate each other?s expression in a compensatory manner at …
Synthesis Of Intracellular And Extracellular Gold Nanoparticles With A Green Machine And Its Antifungal Activity, Nurbanu Gürsoy, Betül Yilmaz Öztürk, İlknur Dağ
Synthesis Of Intracellular And Extracellular Gold Nanoparticles With A Green Machine And Its Antifungal Activity, Nurbanu Gürsoy, Betül Yilmaz Öztürk, İlknur Dağ
Turkish Journal of Biology
Green synthesis method is being increasingly used in the development of safe, stable, and eco-friendly nanostructures with biological resources. In this study, extracellular and intracellular synthesis of gold nanoparticles (AuNPs) was carried out using green algae Chlorella sorokiniana Shihira & R.W. Fresh algae were isolated and identified from Musaözü Pond located in the province of Eskişehir and then extraction process were performed. Optimization studies were studied using pH value, metal salt concentration, and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs …
Helicobacter-Stimulated Il-10-Producing B Cells Suppress Differentiation Of Lipopolysaccharide/Helicobacter Felis-Activated Stimulatory Dendritic Cells, Doğuş Altunöz, Ayça Sayi Yazgan
Helicobacter-Stimulated Il-10-Producing B Cells Suppress Differentiation Of Lipopolysaccharide/Helicobacter Felis-Activated Stimulatory Dendritic Cells, Doğuş Altunöz, Ayça Sayi Yazgan
Turkish Journal of Biology
Regulatory B cells (Bregs) produce antiinflammatory cytokines and inhibits proinflammatory response. Recently, immunosuppressive roles of Bregs in the effector functions of dendritic cells (DCs) were demonstrated. However, cross talk between Bregs and DCs in Helicobacter infection remains unknown. Here, we showed that direct stimulation of bone marrow-derived DCs (BMDCs) with Helicobacter felis (H. felis) antigen upregulates their CD86 surface expression and causes the production of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). Furthermore, prestimulation of DCs with supernatants derived from both Helicobacter-stimulated IL-10- B (Hfstim-IL-10- B) or IL-10+ B (Hfstim-IL-10+) cells suppresses the secretion of …
Il-1r1 Deficiency Impairs Liver Regeneration After 2/3 Partial Hepatectomy In Aged Mice, Deming Li, Ze Wang, Chunyan Zhang, Cunshuan Xu
Il-1r1 Deficiency Impairs Liver Regeneration After 2/3 Partial Hepatectomy In Aged Mice, Deming Li, Ze Wang, Chunyan Zhang, Cunshuan Xu
Turkish Journal of Biology
Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative realtime polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient …
Reconstitution Of Pol Ii (G) Responsive Form Of The Human Mediator Complex, Murat Alper Cevher
Reconstitution Of Pol Ii (G) Responsive Form Of The Human Mediator Complex, Murat Alper Cevher
Turkish Journal of Biology
RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription …
Crosstalk Between Autophagy And Dna Repair Systems, Si̇nem Sarikaya, Hati̇ce Çakir, Devri̇m Gözüaçik, Yunus Akkoç
Crosstalk Between Autophagy And Dna Repair Systems, Si̇nem Sarikaya, Hati̇ce Çakir, Devri̇m Gözüaçik, Yunus Akkoç
Turkish Journal of Biology
Autophagy and DNA repair are two essential biological mechanisms that maintain cellular homeostasis. Impairment of these mechanisms was associated with several pathologies such as premature aging, neurodegenerative diseases, and cancer. Intrinsic or extrinsic stress stimuli (e.g., reactive oxygen species or ionizing radiation) cause DNA damage. As a biological stress response, autophagy is activated following insults that threaten DNA integrity. Hence, in collaboration with DNA damage repair and response mechanisms, autophagy contributes to the maintenance of genomic stability and integrity. Yet, connections and interactions between these two systems are not fully understood. In this review article, current status of the associations …
Polyhydroxyalkanoate Accumulation In Streptomyces Coelicolor Affected By Sco7613 Gene Region, Zeynep Demi̇r Öksüz, Tuğrul Doruk, Nevi̇n Yağci, Sedef Tunca Gedi̇k
Polyhydroxyalkanoate Accumulation In Streptomyces Coelicolor Affected By Sco7613 Gene Region, Zeynep Demi̇r Öksüz, Tuğrul Doruk, Nevi̇n Yağci, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. BLAST search results of the GenBank database argued that SCO7613 could specify a putative polyhydroxyalkanoate synthase (PhaC) responsible for PHA biosynthesis. Deduced amino acid sequence of SCO7613 showed the presence of conserved lipase box like sequence, …
Lipase From New Isolate Bacillus Cereus Ata179: Optimization Of Production Conditions, Partial Purification, Characterization And Its Potential In The Detergent Industry, Eli̇f Demi̇rkan, Aynur Aybey Çeti̇nkaya, Maoulida Abdou
Lipase From New Isolate Bacillus Cereus Ata179: Optimization Of Production Conditions, Partial Purification, Characterization And Its Potential In The Detergent Industry, Eli̇f Demi̇rkan, Aynur Aybey Çeti̇nkaya, Maoulida Abdou
Turkish Journal of Biology
In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4)2HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h. ATA179 strain showed a 51% increase in enzyme production in the modified medium created by optimizing nutritional …
Transcriptome Profiling Of Stevia Rebaudiana Ms007 Revealed Genes Involved In Flower Development, Nurul Hidayah Samsulrizal, Khairul Shahyidi Khadzran, Tamil Sundram Meenakshi Sundram, Zarina Zainuddin, Siti Hajar Nor Shaarani, Nur Sabrina Ahmad Azmi, Sarahani Harun
Transcriptome Profiling Of Stevia Rebaudiana Ms007 Revealed Genes Involved In Flower Development, Nurul Hidayah Samsulrizal, Khairul Shahyidi Khadzran, Tamil Sundram Meenakshi Sundram, Zarina Zainuddin, Siti Hajar Nor Shaarani, Nur Sabrina Ahmad Azmi, Sarahani Harun
Turkish Journal of Biology
Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence, this study aims to identify the genes involved in the flowering of local S. rebaudiana accession MS007 by investigating the transcriptomic data of two stages of growth, before flowering (BF) and after flowering (AF) that were deposited under accession number SRX6362785 and SRX6362784 at the NCBI SRA database. The …
Identification Of Serum Predictors Of N-Acetyl-L-Cysteine And Isoproterenol Induced Remodelling In Cardiac Hypertrophy, Dharaniyambigai Kuberapandian, Victor Arokia Doss
Identification Of Serum Predictors Of N-Acetyl-L-Cysteine And Isoproterenol Induced Remodelling In Cardiac Hypertrophy, Dharaniyambigai Kuberapandian, Victor Arokia Doss
Turkish Journal of Biology
Cardiac hypertrophy (CH), leading to cardiac failure is due to chronic metabolic alterations occurring during cellular stress. Besides the already known relationship between oxidative stress and CH, there are implications of reductive stress leading to CH. This study attempted to develop reductive stress-based CH rat model using n-acetyl-L-cysteine (NAC), a glutathione agonist that was compared with typical isoproterenol (ISO) induced CH model. The main objective was to identify serum metabolites that can serve as potent predictors for seven routine clinical and diagnostic parameters in CH: 3-hydroxybutyrate (3-HB), lactic acid (LA), urea, and ECG-CH parameters (QRS complex, R-amplitude, R-R interval, heart …
Linkage Of Nanosecond Protein Motion With Enzymatic Methyl Transfer By Nicotinamide N-Methyltransferase, Yahui Jing, Yiting Cheng, Fangya Li, Yuping Li, Fan Liu, Jianyu Zhang
Linkage Of Nanosecond Protein Motion With Enzymatic Methyl Transfer By Nicotinamide N-Methyltransferase, Yahui Jing, Yiting Cheng, Fangya Li, Yuping Li, Fan Liu, Jianyu Zhang
Turkish Journal of Biology
Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N1-methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking. Here, wild-type nicotinamide N-methyltransferase together with the mutation at position 20 with Y20F, Y20G, and free tryptophan were carried out to explore the connection between protein dynamics and catalysis using time-resolved fluorescence lifetimes. The results show that wild-type nicotinamide N-methyltransferase prefers …
E-Cadherin Might Be A Stage-Dependent Modulator In Aggressiveness In Pancreatic Cancer Cells, Esra Aydemi̇r Çoban, Di̇dem Teci̇mel, Ezgi̇ Kaşikci, Ömer Faruk Bayrak, Fi̇kretti̇n Şahi̇n
E-Cadherin Might Be A Stage-Dependent Modulator In Aggressiveness In Pancreatic Cancer Cells, Esra Aydemi̇r Çoban, Di̇dem Teci̇mel, Ezgi̇ Kaşikci, Ömer Faruk Bayrak, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Pancreatic ductal adenocarcinoma (PDAC) pathology is known for its uncontrollable progress due to highly invasive characteristics and refractory behavior against existing chemotherapies. The aberrant expression of CDH1 (expresses the protein E-cadherin) is associated with increased overall survival in various cancers, however, E-cadherin expression in PDAC progression has remained elusive. We investigated the impact of exogenously elevated E-cadherin levels on the tumorigenicity of transduced low grade and metastatic PDAC cell lines, Panc-1 and AsPC-1, respectively. Constitutive expression of E-cadherin promoted a more hybrid E/M state in AsPC-1 cells, while it was associated with the acquisition of a more epithelial-like state in …
Comparison Of Anticarcinogenic Properties Of Viburnum Opulus And Its Active Compound P-Coumaric Acid On Human Colorectal Carcinoma, Serdar Karakurt, Gülsüm Abuşoğlu, Zeki̇ye Ceren Arituluk
Comparison Of Anticarcinogenic Properties Of Viburnum Opulus And Its Active Compound P-Coumaric Acid On Human Colorectal Carcinoma, Serdar Karakurt, Gülsüm Abuşoğlu, Zeki̇ye Ceren Arituluk
Turkish Journal of Biology
Resistance to therapeutic agents and the highly toxic side effects of synthetic drugs has spurred new research in the treatment of colon cancer, which has high morbidity and mortality ratios. This study aims to clarify the molecular mechanisms of the anticarcinogenic properties of methanol extract of Viburnum opulus L. (EVO)and its main active compound, trans-p-coumaric acid (p-CA), on human colon cancer cells (DLD-1, HT-29, SW-620, Caco-2) and healthy colon epithelial cells (CCD-18Co). The effects of EVO on controlled cell death (apoptosis) and the cell division cycle were determined by flow cytometry. Alteration in mRNA and protein expressions of switch genes …
Caffeic Acid Phenethyl Ester Induces Apoptosis In Colorectal Cancer Cells Via Inhibition Of Survivin, Ceren Sari, Ceren Sümer, Fi̇gen Celep Eyüpoğlu
Caffeic Acid Phenethyl Ester Induces Apoptosis In Colorectal Cancer Cells Via Inhibition Of Survivin, Ceren Sari, Ceren Sümer, Fi̇gen Celep Eyüpoğlu
Turkish Journal of Biology
Colorectal cancer is one of the most common types of cancer. Drug resistance and drug-induced damage of healthy tissues are major obstacles in cancer treatment. Therefore, to develop efficient anticancer therapy, it is necessary to find compounds that affect tumor cells, but do not exhibit toxicity to healthy cells. Caffeic acid phenethyl ester (CAPE) has been demonstrated to have anticancer properties in many types of cancer. In this study, the cytotoxic and apoptotic effects of CAPE on the RKO colorectal cancer cell line and CCD 841-CoN normal colorectal cell line was investigated. In addition, changes in the survivin expression were …
Anxiolytic, Antioxidant, And Neuroprotective Effects Of Goji Berry Polysaccharides In Ovariectomized Rats: Experimental Evidence From Behavioral, Biochemical, And Immunohistochemical Analyses, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Hayri̇ye Soytürk, Bi̇hter Gökçe Bozat
Anxiolytic, Antioxidant, And Neuroprotective Effects Of Goji Berry Polysaccharides In Ovariectomized Rats: Experimental Evidence From Behavioral, Biochemical, And Immunohistochemical Analyses, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Hayri̇ye Soytürk, Bi̇hter Gökçe Bozat
Turkish Journal of Biology
Recent studies have indicated that polysaccharides, the main component of the Lycium barbarum L. fruit, have beneficial effects (e.g., anxiolytic, antioxidant, and neuroprotective) on humans and rodents. However, the effects of different dosages of such polysaccharides on ovariectomized rats and their underlying mechanisms in the brain have not been evaluated in the literature. Here, we aimed to evaluate the effects of the high and low doses of polysaccharides obtained from Lycium barbarum fruits (HD-LBP and LD-LBP, respectively) on anxious behaviors via behavioral (using the OFT and EPM), biochemical (using ELISA), and immunohistochemical (using immunohistochemical staining) measures in detail. Two weeks …
Upregulation Of Psmd4 Gene By Hypoxia In Prostate Cancer Cells, Sümeyye Aydogan Türkoğlu, Gi̇zem Dayi, Feray Köçkar
Upregulation Of Psmd4 Gene By Hypoxia In Prostate Cancer Cells, Sümeyye Aydogan Türkoğlu, Gi̇zem Dayi, Feray Köçkar
Turkish Journal of Biology
Ubiquitin-proteasome pathways have a crucial role in tumor progression. PSMD4 (Rpn10, 26S proteasome non-ATPase subunit 4), which is a subunit of the regulatory particle, is a major ubiquitin (Ub) receptor of 26S proteasome. PSMD4 overexpression has been observed in colon carcinoma, hepatocellular carcinoma, and breast cancer. In this work, we elucidated the effect of hypoxia on PSMD4 gene expression in prostate cancer cells (PC3). Chemically mimicked hypoxia drastically upregulated PSMD4 gene expression at both mRNA and protein levels. Transient transfection experiments indicated that all promoter fragments were active in PC3 cells. Hypoxia increased transcriptional activity of all PSMD4 promoter constructs. …
Single Nucleotide Polymorphisms In Micrornas Action As Biomarkers For Breast Cancer, Thanh Thi Ngoc Nguyen, Minh Thi Hong Tran, Vy Thi Lan Nguyen, Uyen Doan Phuong Nguyen, Giang Dien Thanh Nguyen, Luan Huu Huynh, Hue Thi Nguyen
Single Nucleotide Polymorphisms In Micrornas Action As Biomarkers For Breast Cancer, Thanh Thi Ngoc Nguyen, Minh Thi Hong Tran, Vy Thi Lan Nguyen, Uyen Doan Phuong Nguyen, Giang Dien Thanh Nguyen, Luan Huu Huynh, Hue Thi Nguyen
Turkish Journal of Biology
MicroRNAs (miRNAs) have been recently described as small noncoding RNAs that are involved in numerous crucial physiological processes, such as cell cycles, differentiation, development, and metabolism. Thus, dysregulation of these molecules could lead to several severe disorders, including breast cancer (BC). Ongoing investigations in malignant growth diagnostics have distinguished miRNAs as promising disease biomarkers. As with any other mRNAs, single nucleotide polymorphisms (SNPs) in DNA sequence encoding for miRNA (miR-SNPs) indeed lead to potential changes in the function of miRNA. In this study, miR-SNPs located in different miRNA sequence regions, which have been associated with BC in different ways, and …
Regulation Of E2f1 Activity Via Pka-Mediated Phosphorylations, Mustafa Gökhan Ertosun, Sayra Di̇lmaç, Fatma Zehra Hapi̇l Zevkli̇ler, Gamze Tanriöver, Sadi̇ Köksoy, Osman Ni̇dai̇ Özeş
Regulation Of E2f1 Activity Via Pka-Mediated Phosphorylations, Mustafa Gökhan Ertosun, Sayra Di̇lmaç, Fatma Zehra Hapi̇l Zevkli̇ler, Gamze Tanriöver, Sadi̇ Köksoy, Osman Ni̇dai̇ Özeş
Turkish Journal of Biology
No abstract provided.
Effects Of Moderate High Temperature And Uv-B On Accumulation Of Withanolides And Relative Expression Of The Squalene Synthase Gene In Physalis Peruviana, Günce Şahi̇n, Murat Telli̇, Ercan Selçuk Ünlü, Fatma Pehli̇van Karakaş
Effects Of Moderate High Temperature And Uv-B On Accumulation Of Withanolides And Relative Expression Of The Squalene Synthase Gene In Physalis Peruviana, Günce Şahi̇n, Murat Telli̇, Ercan Selçuk Ünlü, Fatma Pehli̇van Karakaş
Turkish Journal of Biology
Physalis peruviana L. (Cape gooseberry) is a source for a variety of phytocompounds such as withanolides, withanone, withaferin A, and withanolide A. These withanolides are high-value drug candidates due to their various pharmacological properties. To meet the increasing demands for these compounds, plant cell technology offers a reliable alternative. Exogenous addition of elicitors is considered the most effective strategy for enhanced production of secondary metabolites. In this study, we investigated changes in withanolide accumulation and characterized the gene expression level changes of squalene synthase enzyme in P. peruviana shoot cultures exposed to mild nonlethal heat stress (45°C for 2 and …
In Vitro Tooth-Shaped Scaffold Construction By Mimicking Late Bell Stage, Paki̇ze Nesli̇han Taşli, Gül Merve Yalçin Ülker, Alev Cumbul, Ünal Uslu, Şahi̇n Yilmaz, Batuhan Turhan Bozkurt, Fi̇kretti̇n Şahi̇n
In Vitro Tooth-Shaped Scaffold Construction By Mimicking Late Bell Stage, Paki̇ze Nesli̇han Taşli, Gül Merve Yalçin Ülker, Alev Cumbul, Ünal Uslu, Şahi̇n Yilmaz, Batuhan Turhan Bozkurt, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Neogenesis of osseous and ligamentous interfacial structures is essential for the regeneration of large oral or craniofacial defects. However, current treatment strategies are inadequate in renewing supporting tissues of teeth after trauma, chronic infections or surgical resection. Combined use of 3D scaffolds with stem cells became a promising treatment option for these injuries. Matching different scaffolding materials with different tissues can induce the correct cytokines and the differentiation of cells corresponding to that particular tissue. In this study, a hydroxyapatite (HA) based scaffold was used together with human adipose stem cells (hASCs), human bone marrow stem cells (hBMSCs) and gingival …
Adjuvant Potency Of Astragaloside Vii Embedded Cholesterol Nanoparticles For H3n2 Influenza Vaccine, Rükan Genç, Ni̇lgün Yakuboğullari, Ayşe Nalbantsoy, Fethi̇ye Çöven, Erdal Bedi̇r
Adjuvant Potency Of Astragaloside Vii Embedded Cholesterol Nanoparticles For H3n2 Influenza Vaccine, Rükan Genç, Ni̇lgün Yakuboğullari, Ayşe Nalbantsoy, Fethi̇ye Çöven, Erdal Bedi̇r
Turkish Journal of Biology
Adjuvants are substances that increase the immune response to a given antigen. In the development of novel vaccine adjuvants/ systems, saponins are one of the most attractive molecules due to their altered immunomodulatory activities. In this study, we tried to develop PEG (polyethylene glycol)/cholesterol-based lipid nanoparticles (LNPs) to deliver the Astragaloside VII (AST-VII) and potentiate adjuvant properties of AST-VII for the influenza vaccine. In the formation of PEG/cholesterol/AST-VII-based LNPs (PEG300: Chol-AST-VII LNPs), 3 different primary solvents (acetone, ethanol, and chloroform) were evaluated, employing their effects on hydrodynamic particle size, distribution, surface chemistry, and colloidal stability. Prepared nanoparticles were simply admixtured …