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Articles 151 - 180 of 1681
Full-Text Articles in Biology
Emerging Applications Of 3d Engineered Constructs From Plant Seed Extracts, Sedat Odabaş, Bora Gari̇pcan, Rihab Ksouri
Emerging Applications Of 3d Engineered Constructs From Plant Seed Extracts, Sedat Odabaş, Bora Gari̇pcan, Rihab Ksouri
Turkish Journal of Biology
Seeds, by-products derived from various plants such as mango, quince, and apples, are considered waste, though they have emerging commercial potential, and have been used in biological, industrial, and physiological research. Seed-derived natural macromolecules- mainly polysaccharides, mucilage, gums, and cellulose-have physicochemical and structural diversification, giving the potential for forming gels, texturing, thickening, and providing interfacial adsorption. Seed-derived natural macromolecules have been widely used during the last few years in cell research and tissue engineering applications. Their widespread approachability and safety, high rate of biodegradability, biocompatibility, supporting cell proliferation, and extracellular matrix synthesis are the main properties making plant seed derivatives …
Linc00460 Mediates Hmga2 Expression Through Binding To Mirna-143-5p Competitively In Gastric Carcinoma, Xuqing Zhu, Yanli Xiang, Feifei Mo, Lingling Jin
Linc00460 Mediates Hmga2 Expression Through Binding To Mirna-143-5p Competitively In Gastric Carcinoma, Xuqing Zhu, Yanli Xiang, Feifei Mo, Lingling Jin
Turkish Journal of Biology
Background: Compelling evidence has manifested a strong association between aberrant expression of long noncoding RNAs (lncRNAs) and gastric carcinoma (GC) development. Nonetheless, biological impacts of differentially expressed lncRNAs (DElncRNAs) on GC are not scrutinized. Methods: Bioinformatics methods were employed for differential expression analysis and target gene prediction. MTT, colony formation, and Transwell methods were implemented for GC cell proliferation, migration, and invasion assessment. Western blot was implemented to test the protein level. The binding of genes was tested with dual-luciferase and RNA binding protein immunoprecipitation (RIP) approaches. Results: Noticeably high level of LINC00460 was observed in GC tissues and cells. …
Boquila: Ngs Read Simulator To Eliminate Read Nucleotide Bias In Sequence Analysis, Ümi̇t Akköse, Ogün Adebali̇
Boquila: Ngs Read Simulator To Eliminate Read Nucleotide Bias In Sequence Analysis, Ümi̇t Akköse, Ogün Adebali̇
Turkish Journal of Biology
Sequence content is heterogeneous throughout genomes. Therefore, genome-wide next-generation sequencing (NGS) reads biased towards specific nucleotide profiles are affected by the genome-wide heterogeneous nucleotide distribution. Boquila generates sequences that mimic the nucleotide profile of true reads, which can be used to correct the nucleotide-based bias of genome-wide distribution of NGS reads. Boquila can be configured to generate reads from only specified regions of the reference genome. It also allows the use of input DNA sequencing to correct the bias due to the copy number variations in the genome. Boquila uses standard file formats for input and output data, and it …
Cryogenic X-Ray Crystallographic Studies Of Biomacromolecules At Turkish Light Source "Turkish Delight", Necati̇ Atalay, Enver Kami̇l Akcan, Mehmet Gül, Esra Ayan, Ebru Destan, Fatma Betül Ertem Kuzucu, Nuretti̇n Tokay, Bariş Çakilkaya, Zeli̇ş Nergi̇z, Gözde Karakadioğlu Usta, Abdullah Kepceoğlu, İlki̇n Yapici, Bi̇lge Tosun, Ni̇lüfer Baldir, Günseli̇ Yildirim, J Austin Johnson, Ömür Güven, Alaleh Shafiei, Nazli Eylül Arslan, Merve Yilmaz, Cahi̇ne Kulakman, Seyi̇de Seda Paydos, Seynep Sena Çi̇nal, Kardelen Şabanoğlu, Ayşegül Pazarçevi̇ren, Ayşenur Yilmaz, Başak Canbay, Bengi̇su Aşçi, Esra Kartal, Serra Tavli, Mehmet Çaliseki̇, Günce Göç, Ari̇f Mermer, Gamze Yeşi̇lay, Sevde Altuntaş, Hiroshi Tateishi, Masami Otsuka, Mikako Fujita, Şaban Teki̇n, Hali̇li̇brahi̇m Çi̇ftçi̇, Serdar Durdaği, Gi̇zem Di̇nler Doğanay, Ezgi̇ Karaca, Burcu Kaplan Türköz, Burak Veli̇ Kabasakal, Ahmet Kati, Hasan Demi̇rci̇
Cryogenic X-Ray Crystallographic Studies Of Biomacromolecules At Turkish Light Source "Turkish Delight", Necati̇ Atalay, Enver Kami̇l Akcan, Mehmet Gül, Esra Ayan, Ebru Destan, Fatma Betül Ertem Kuzucu, Nuretti̇n Tokay, Bariş Çakilkaya, Zeli̇ş Nergi̇z, Gözde Karakadioğlu Usta, Abdullah Kepceoğlu, İlki̇n Yapici, Bi̇lge Tosun, Ni̇lüfer Baldir, Günseli̇ Yildirim, J Austin Johnson, Ömür Güven, Alaleh Shafiei, Nazli Eylül Arslan, Merve Yilmaz, Cahi̇ne Kulakman, Seyi̇de Seda Paydos, Seynep Sena Çi̇nal, Kardelen Şabanoğlu, Ayşegül Pazarçevi̇ren, Ayşenur Yilmaz, Başak Canbay, Bengi̇su Aşçi, Esra Kartal, Serra Tavli, Mehmet Çaliseki̇, Günce Göç, Ari̇f Mermer, Gamze Yeşi̇lay, Sevde Altuntaş, Hiroshi Tateishi, Masami Otsuka, Mikako Fujita, Şaban Teki̇n, Hali̇li̇brahi̇m Çi̇ftçi̇, Serdar Durdaği, Gi̇zem Di̇nler Doğanay, Ezgi̇ Karaca, Burcu Kaplan Türköz, Burak Veli̇ Kabasakal, Ahmet Kati, Hasan Demi̇rci̇
Turkish Journal of Biology
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and …
Sdf-1 Induces Directional Chemotaxis Of Endogenous Bmscs At The Intervertebral Fusion Site And Promotes Osteogenic Differentiation By Regulating Wnt/Ss-Catenin In The Bone Marrow Chimera Spinal Intervertebral Fusion Mouse Model, Qiwen Zhang, Ning Liang, Bin He, Siyou Wu, Depeng Wen, Xiaoyong Tang, Xiongcheng Shen
Sdf-1 Induces Directional Chemotaxis Of Endogenous Bmscs At The Intervertebral Fusion Site And Promotes Osteogenic Differentiation By Regulating Wnt/Ss-Catenin In The Bone Marrow Chimera Spinal Intervertebral Fusion Mouse Model, Qiwen Zhang, Ning Liang, Bin He, Siyou Wu, Depeng Wen, Xiaoyong Tang, Xiongcheng Shen
Turkish Journal of Biology
Clinical observations show that the current spinal fusion with internal fixation has a nonfusion rate of 5%-35%; however, methods to promote spinal fusion are limited. This study aimed to investigate the role of SDF-1-induced directional chemotaxis of BMSCs in bone marrow chimera spinal intervertebral fusion mouse model. BMSCs were isolated from bone marrow and identified by detecting CD44/CD34 positive cells. BMSCs (GFP-BMSCs) were labeled with GFP for tracking in vivo. Mice were inoculated with GFP-BMSCs to construct bone marrow chimera spinal intervertebral fusion model, which were divided into BM-SIF model, BM-SIF+SDF-1, BM-SIF+SDF-1-Anta group. The callus area of intervertebral fusion site …
Hydroquinidine Displays A Significant Anti-Carcinogenic Activity In Breast And Ovarian Cancer Cells Via Inhibiting Cell-Cycle And Stimulating Apoptosis, Mervenur Yavuz, Betül Şahi̇n, Ahmet Tarik Baykal, Turan Demi̇rcan
Hydroquinidine Displays A Significant Anti-Carcinogenic Activity In Breast And Ovarian Cancer Cells Via Inhibiting Cell-Cycle And Stimulating Apoptosis, Mervenur Yavuz, Betül Şahi̇n, Ahmet Tarik Baykal, Turan Demi̇rcan
Turkish Journal of Biology
Breast and ovarian cancers are women's most commonly diagnosed cancers. Seeking an efficient anticarcinogenic compound is still a top priority regarding the aggressiveness of these cancers and the limited benefit of current therapies. Hydroquinidine (HQ) is a natural alkaloid used in arrhythmia and Brugada syndrome. As an ion channel blocker, HQ exhibits its activity by altering ion gradient and membrane potential. Considering the growing evidence of ion channel blockers' antineoplastic potential, we were prompted to test HQ's effect on breast and ovarian cancers. MCF-7 and SKOV-3 cell lines were used to inspect how HQ acts on survival, clonogenicity, migration, tumorigenicity, …
Astragaloside Iv Improves Renal Function And Alleviates Renal Damage And Inflammation In Rats With Chronic Glomerulonephritis, Dong Zhang, Zongying Li, Yuan Gao, Hailing Sun
Astragaloside Iv Improves Renal Function And Alleviates Renal Damage And Inflammation In Rats With Chronic Glomerulonephritis, Dong Zhang, Zongying Li, Yuan Gao, Hailing Sun
Turkish Journal of Biology
From Astragalus membranaceus (Fisch.) Bge.var. mongholicus (Bge.) Hsiao, astragaloside IV (AS-IV), a saponin can be purified and is considered traditional Chinese medicine. The purpose of this study was to evaluate the AS-IV-mediated mechanism on chronic glomerulonephritis (CGN). A cationic bovine serum albumin-induced CGN rat model was established and 10, 15, or 20 mg/kg of AS-IV was administered to measure renal function and inflammatory infiltration. Influences of AS-IV on proliferation, cell cycle, and inflammation of LPS-induced rat mesangial cells (RMCs) were determined. The results demonstrated that AS-IV alleviated renal dysfunction, renal lesions, and inflammation in CGN rats. AS-IV prolonged the G0-G1 …
A Ph-Responsive Shell-Sheddable Polymeric Nanoparticles As The Photodynamic Drug Carrier: Synthesis, Characterization, And In Vitro Studies, Jing Lu
Turkish Journal of Biology
Shell-sheddable nanoparticles, composed of amphiphilic blockpolymers, have emerged as an attractive vehicle for the site-specific delivery of therapeutic agents. In this study, pH-responsive sheddable copolymers bearing an orthoester linker were synthesized via the ring-opening polymerization between γ-benzyl-L-glutamate N-carboxyanhydride and orthoester-bearing poly (ethylene glycol) macroinitiator (PEG-pH-NH2). The obtained poly (ethylene glycol)-b-poly(γ-benzyl-L-glutamate) (PEG-PBLG) could form stable nanoparticles in aqueous solutions due to the amphiphilic nature of the block copolymers. The PEG-PBLG-based nanoparticle exhibited good stability in physiological conditions (pH 7.4), whereas the nanoparticle was disassembled under acidic conditions (pH 5.0). The nanoparticles could encapsulate a photosensitizer, protophorphyrin IX …
Methionine Restriction And Cancer Treatment: A Systems Biology Study Of Yeast To Investigate The Possible Key Players, Esra Börklü
Methionine Restriction And Cancer Treatment: A Systems Biology Study Of Yeast To Investigate The Possible Key Players, Esra Börklü
Turkish Journal of Biology
Background/aim: Dietary restriction, mainly carbon and/or methionine restriction are among the upcoming supporting interventions along with chemotherapy in various cancers. Although dietary restriction has been proven to be beneficial, the main cellular machineries affected by its administration lacks deeper information considerably, a notable pitfall in its use as a personalized nutritional approach. Materials and methods: In this study, cellular effects of methionine restriction on a yeast model are explored via systems biology approaches. The methionine biosynthesis network, constructed by integrating interaction data with gene ontology terms, was analysed topologically, and proved to be informative about the intertwined relationship of methionine …
The Protective Impact Of Growth Hormone Against Rotenone-Induced Apoptotic Cell Death Via Acting On Endoplasmic Reticulum Stress And Autophagy Axis, Özge Rencüzoğullari, Selay Tornaci, Yağmur Çeli̇k, Nayat Narot Taş, Pinar Obakan Yerli̇kaya, Eli̇f Damla Arisan, Ajda Çoker Gürkan
The Protective Impact Of Growth Hormone Against Rotenone-Induced Apoptotic Cell Death Via Acting On Endoplasmic Reticulum Stress And Autophagy Axis, Özge Rencüzoğullari, Selay Tornaci, Yağmur Çeli̇k, Nayat Narot Taş, Pinar Obakan Yerli̇kaya, Eli̇f Damla Arisan, Ajda Çoker Gürkan
Turkish Journal of Biology
Human growth hormone (GH) is crucial modulator of cellular metabolisms, including cell proliferation and organ development, by stimulating insulin-like growth factor-1 (IGF-1), which has various functions such as cell proliferation, tissue growth, survival, or neuroprotection. Therefore, GH is implicated as a critical player in the cell and can enhance neurogenesis and provide neuroprotection during the treatment of neurological diseases such as Parkinson's disease (PD). In this study, the neuroprotective role of GH was investigated in rotenone-induced PD models for the first time. Both SH-SY5Y and SK-N-AS neuroblastoma cells were exposed to rotenone to mimic PD pathogenesis as stated in previous …
Rpi-1 (Human Dcdc2) Displays Functional Redundancy With Nephronophthisis 4 In Regulating Cilia Biogenesis In C. Elegans, Oktay İsmai̇l Kaplan
Rpi-1 (Human Dcdc2) Displays Functional Redundancy With Nephronophthisis 4 In Regulating Cilia Biogenesis In C. Elegans, Oktay İsmai̇l Kaplan
Turkish Journal of Biology
Projecting from most cell surfaces, cilia serve as important hubs for sensory and signaling processes and have been linked to a variety of human disorders, including Bardet-Biedl Syndrome (BBS), Meckel-Gruber Syndrome (MKS), Nephronophthisis (NPHP), and Joubert Syndrome, and these diseases are collectively known as a ciliopathy. DCDC2 is a ciliopathy protein that localizes to cilia; nevertheless, our understanding of the role of DCDC2 in cilia is still limited. We employed C. elegans to investigate the function of C. elegans RPI-1, a Caenorhabditis elegans ortholog of human DCDC2, in cilia and found that C. elegans RPI-1 localizes to the entire ciliary …
Effects Of Growth Factor Deprivation On Mkn-45 Spheroid Cells, Özlem Türksoy, Gökhan Terzi̇oğlu
Effects Of Growth Factor Deprivation On Mkn-45 Spheroid Cells, Özlem Türksoy, Gökhan Terzi̇oğlu
Turkish Journal of Biology
Background/aim: Serum and growth factor deprivation, a common cellular stressor in solid tumors, arises upon irradiation, chemotherapy, and antiangiogenesis. Spheroid body culture aims to enrich cancer stem cells by using low attachment conditions and some growth factors, such as basic fibroblast growth factor and epidermal growth factor to support the spheroid formation in serum-free spheroid culture. However, spheroid culture without any growth factors can imitate the tumor environment more realistically. In this study, we aimed to identify the effect of growth factor deprivation on the MKN-45 gastric cancer cell line in terms of stemness characteristics. Materials and methods: The spheroids …
Application Of Scanning Acoustic Microscopy For Evaluation Of Mmp Activation In Multiple Cancer Cell Lines With A Smart Probe, Hasan Ozan Otaş, Nasi̇re Uluç, İrem Demi̇rkan, Ayli̇n Alkan, Açelya Yilmazer, Seda Yaşa Ercan, Davod Khalafkhany, Nesri̇n Özören, Mehmet Burçi̇n Ünlü
Application Of Scanning Acoustic Microscopy For Evaluation Of Mmp Activation In Multiple Cancer Cell Lines With A Smart Probe, Hasan Ozan Otaş, Nasi̇re Uluç, İrem Demi̇rkan, Ayli̇n Alkan, Açelya Yilmazer, Seda Yaşa Ercan, Davod Khalafkhany, Nesri̇n Özören, Mehmet Burçi̇n Ünlü
Turkish Journal of Biology
Background/aim: Matrix metalloproteinases (MMPs) play an important role in the evaluation of many cancer types; however, the detection usually presents a challenge. Further assays for a better understanding of the fundamental roles of MMPs in pathophysiology are still needed. We aimed to use an activatable probe in scanning acoustic microscopy (SAM) to evaluate acoustically if the probe can aid the visualization of the effects of in vitro MMP activity. Materials and methods: We applied scanning acoustic impedance microscopy to obtain acoustic impedance maps of the cell line models of HT1080, THP-1, and SK-MEL-28 with and without MMPSense 680 probe incubation. …
Transcriptomic Profiling Of Mice Brain Under Bex3 Regulation, Zhimin Xu, Noor Bahadar, Yingxin Zhang, Shuang Tan, Zhongze Wang, Bingyi Ren, Shitao Liu, Huanyan Dai, Yaowu Zheng, Bing Han
Transcriptomic Profiling Of Mice Brain Under Bex3 Regulation, Zhimin Xu, Noor Bahadar, Yingxin Zhang, Shuang Tan, Zhongze Wang, Bingyi Ren, Shitao Liu, Huanyan Dai, Yaowu Zheng, Bing Han
Turkish Journal of Biology
BEX family genes are expressed in various tissues and play significant roles in neuronal development. A mouse model of Bex3 gene knock-out was generated in this study, using the CRISPR-Cas9 system. Transcriptomic analysis of the brain was performed to identify genes and pathways under Bex3 regulation. Essential biological functions under the control of Bex3 related to brain development were identified. Ninety-five genes were differentially expressed under Bex3-/- regulation, with 53 down and 42 up. Among down-regulated genes, LOC102633156 is a member of zf-C2H2, Xlr3a is an X-linked lymphocyte regulated gene, LOC101056144 is a hippocampal related gene, 2210418O10Rik and Fam205a3 are …
Noncoding Rnas In Apoptosis: Identification And Function, Özge Tüncel, Merve Kara, Bi̇lge Yaylak, İpek Erdoğan, Bünyami̇n Akgül
Noncoding Rnas In Apoptosis: Identification And Function, Özge Tüncel, Merve Kara, Bi̇lge Yaylak, İpek Erdoğan, Bünyami̇n Akgül
Turkish Journal of Biology
Apoptosis is a vital cellular process that is critical for the maintenance of homeostasis in health and disease. The derailment of apoptotic mechanisms has severe consequences such as abnormal development, cancer, and neurodegenerative diseases. Thus, there exist complex regulatory mechanisms in eukaryotes to preserve the balance between cell growth and cell death. Initially, protein-coding genes were prioritized in the search for such regulatory macromolecules involved in the regulation of apoptosis. However, recent genome annotations and transcriptomics studies have uncovered a plethora of regulatory noncoding RNAs that have the ability to modulate not only apoptosis but also many other biochemical processes …
Modeling Of C-Terminal Tail Of Human Sting And Its Interaction With Tank-Binding Kinase 1, Rahaf Ata Ouda Al-Masri, Hajara Audu-Bida, Şebnem Eşsi̇z
Modeling Of C-Terminal Tail Of Human Sting And Its Interaction With Tank-Binding Kinase 1, Rahaf Ata Ouda Al-Masri, Hajara Audu-Bida, Şebnem Eşsi̇z
Turkish Journal of Biology
Stimulator of interferon genes (STING) plays a significant role in a cell's intracellular defense against pathogens or self-DNA by inducing inflammation or apoptosis through a pathway known as cGAS-cGAMP-STING. STING uses one of its domains, the C-terminal tail (CTT) to recruit the members of the pathway. However, the structure of this domain has not been solved experimentally. STING conformation is open and more flexible when inactive. When STING gets activated by cGAMP, its conformation changes to a closed state covered by 4 beta-sheets over the binding site. This conformational change leads to its binding to Tank-binding kinase 1 (TBK1). TBK1 …
Ubiquitin Specific Protease 7 Maintains Pluripotency Of Mouse Embryonic Stem Cells Through Stabilization Of Ss-Catenin, Taha Bartu Hayal
Ubiquitin Specific Protease 7 Maintains Pluripotency Of Mouse Embryonic Stem Cells Through Stabilization Of Ss-Catenin, Taha Bartu Hayal
Turkish Journal of Biology
Embryonic stem cells (ESCs), which are derived from the undifferentiated inner cell mass of the embryo, can differentiate every cell type of the body regarding their pluripotency. Therefore, human or mouse ESCs can be used as an unlimited cell source for numerous researches or therapeutical approaches. However, pluripotency maintenance of ESCs during in vitro culture is challenging because of their endless differentiation capacity. In the current study, the effect of USP7 on pluripotency maintenance of mouse ESCs (mESCs) has been investigated with the help of cell viability assay, morphological analysis, alkaline phosphatase (ALP) staining, qPCR analysis, and Western Blotting. 600 …
P110Α Activity At The M-To-G1 Transition Is Critical For Cellular Proliferation And Reentry Into The Cell Cycle, Onur Çi̇zmeci̇oğlu
P110Α Activity At The M-To-G1 Transition Is Critical For Cellular Proliferation And Reentry Into The Cell Cycle, Onur Çi̇zmeci̇oğlu
Turkish Journal of Biology
Phosphoinositide 3-kinase (PI3K) signaling pathway is essential for normal physiology and is impaired in diseases such as premalignant hyperproliferative disorders, primary immunodeficiency, metabolic disorders, and cancer. Although the core PI3K pathway components are known today, a long-standing gap in our knowledge of PI3K signaling concerns how distinct PI3K isoforms and their activity patterns contribute to the functional consequences of pathway upregulation. In order to address this issue, we devised a molecular genetic cell model, which allowed temporal regulation of the indispensable PI3K isoform, p110α in distinct stages of the cell cycle. We found that late M and early G1 …
Cytoplasmically Localized Trna-Derived Fragments Inhibit Translationin Drosophila S2 Cells, Syed Muhammad Hamid, Bünyami̇n Akgül
Cytoplasmically Localized Trna-Derived Fragments Inhibit Translationin Drosophila S2 Cells, Syed Muhammad Hamid, Bünyami̇n Akgül
Turkish Journal of Biology
Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 …
Sirna-Mediated Stathmin1 Silencing Inhibits Proliferation Of Prostate Carcinoma Cell Line, Asude Aksoy, Safi̇ye Selcen Vi̇cdanli, Ebru Önalan, Ahmet Tektemur, Gökhan Artaş, Asuman Orhan Varoğlu, Mustafa Koç
Sirna-Mediated Stathmin1 Silencing Inhibits Proliferation Of Prostate Carcinoma Cell Line, Asude Aksoy, Safi̇ye Selcen Vi̇cdanli, Ebru Önalan, Ahmet Tektemur, Gökhan Artaş, Asuman Orhan Varoğlu, Mustafa Koç
Turkish Journal of Biology
Stathmin1 (STMN1) has been proposed as a possible prognostic marker and a potential therapeutic target for some cancers. We aimed to analyze the changes in autophagy, invasion, apoptosis-related genes in prostate cancer (PCa) cell line (PC-3), after small interfering RNA (siRNA)-mediated STMN1 silencing, and also the relationships of STMN1 expression, clinicopathological parameters, and survival (OS) in PCa cases. The STMN1 expressions were analyzed, immunohistochemically, in formalin-fixed paraffin-embedded 75 PCa and 15 benign prostatic hypertrophy (BPH) tissues. The correlation between the levels of expression STMN1, clinicopathological features, and OS was determined in PCa cases. The siRNA-mediated STMN1 incubated PC-3 cells were …
Transcriptome Sequencing Reveals Circrna Expression Profile In Parkinson's Disease-Like Mice After Aerobic Exercise, Tianlun Fan, Xiating Li, Chuan Fu, Lichun Sun
Transcriptome Sequencing Reveals Circrna Expression Profile In Parkinson's Disease-Like Mice After Aerobic Exercise, Tianlun Fan, Xiating Li, Chuan Fu, Lichun Sun
Turkish Journal of Biology
Parkinson's disease (PD) is a common complex neurodegenerative disease, and aerobic exercise (EX) has potential to improve motor dysfunction. This study aimed to explore whether EX acts on PD in mice mode. Mice were administered 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (MPTP) and subjected to a 4-week physical exercise regimen (EX-PD group) and underwent RNA-Seq. Here, MPTP caused PD, which was characterized by neuron shrinkage and behavioral deficits, whereas EX improved PD by rescuing neuronal survival and motor function in mice. Moreover, circRNA expression profiles identified a total of 142 differentially expressed circRNAs (DEcircRNAs) between PD and EX-PD group. These DEcircRNAs were mainly involved …
Recombinant Ahpc Antigen From Mycobacterium Bovis Boosts Bcg-Primed Immunity In Mice, Özgün Firat Düzenli̇, Sezer Okay, İnci̇ Kazkayasi Bölük, Ayşe Fi̇li̇z Öner
Recombinant Ahpc Antigen From Mycobacterium Bovis Boosts Bcg-Primed Immunity In Mice, Özgün Firat Düzenli̇, Sezer Okay, İnci̇ Kazkayasi Bölük, Ayşe Fi̇li̇z Öner
Turkish Journal of Biology
Tuberculosis (TB) is still one of the most common infectious diseases around the world despite the widespread use of BCG (bacille Calmette-Guerin) strain of Mycobacterium bovis as a vaccine. This vaccine does not always protect people from TB, and, thus, new effective vaccines or vaccination strategies are being investigated. In this study, alkyl hydroperoxide reductase (AhpC) from M. bovis was evaluated as a new candidate vaccine antigen against TB in BALB/c mice model. The ahpC gene was amplified from M.bovis genome, cloned, and expressed in Escherichia coli. Vaccine antigen AhpC was formulated with Montanide ISA 61 VG, an oil-based emulsion …
Design And In Vitro, In Vivo Evaluation Of Antioxidant Bioadhesive Gelsfor Burn Treatment, Göksel Gökçe, Si̇nem Yaprak Karavana, Hüsnü Alper Bağriyanik, Çeti̇n Pekçeti̇n, Evren Alğin Yapar, Gülşen Aybar Tural, Evren Homan Gökçe
Design And In Vitro, In Vivo Evaluation Of Antioxidant Bioadhesive Gelsfor Burn Treatment, Göksel Gökçe, Si̇nem Yaprak Karavana, Hüsnü Alper Bağriyanik, Çeti̇n Pekçeti̇n, Evren Alğin Yapar, Gülşen Aybar Tural, Evren Homan Gökçe
Turkish Journal of Biology
Burn wounds are frequently encountered health problems, which need a new treatment approach especially in terms of good patient compliance. Availability of use of antioxidant agents and bio-adhesive gels in tissue healing can be an alternative as a new approach for wound healing. Antioxidant taurine containing bio-adhesive gels were prepared by using carbopol (CP) 940 and 934. Rheological and texture analyses were carried out on bio-adhesive gels for in vitro characterization. Wound model on Wistar rats was used to evaluate the in vivo evaluation of gels. Rheological and texture analyses showed that a carbopol bioadhesive gel has acceptable topically use …
A Sweet Way To Increase The Metabolic Activity And Migratory Response Of Wound-Related Cells: Deoxy-Sugar Incorporated Natural Polymeric Fibres As A Potential Bioactive Wound Patch, Serkan Di̇ki̇ci̇
Turkish Journal of Biology
The selection of a wound dressing is crucial for successful wound management. Conventional dressings are preferable for the treatment of simple wounds. However, a bioactive wound dressing that supports wound management and accelerates the healing process is required when it comes to treating non-self-healing wounds. 2-deoxy-D-ribose (2dDR) is a small deoxy sugar that naturally occurs in human body. Although we have previously demonstrated that 2dDR can be used to induce neovascularisation and accelerates wound healing in vitro and in vivo, the literature on small sugars is conflicting, and the knowledge on how 2dDR achieves its biological activity is very limited. …
Point-Of-Care Diagnosis Of Cervical Cancer: Potential Protein Biomarkersin Cervicovaginal Fluid, Büşra Köse, Özgüç Takmaz, Mete Güngör, Ahmet Tarik Baykal
Point-Of-Care Diagnosis Of Cervical Cancer: Potential Protein Biomarkersin Cervicovaginal Fluid, Büşra Köse, Özgüç Takmaz, Mete Güngör, Ahmet Tarik Baykal
Turkish Journal of Biology
Cervical cancer (CxCa) is preventable and treatable via vaccination and screening. Cervicovaginal fluid (CVF) represents the physiological components of the female genital tract. These components are suitable to be utilized for clinical purposes, therefore, making CVF a suitable material for disease screening approaches. Due to high false-negative result rates and low attendance of current expensive routine CxCa screening methods, it has become more important to develop a point-of-care (POC) screening method that every single woman could reach worldwide. For this purpose, various self-usage apparatus have been developed for screening of the human papilloma virus (HPV) infection. Furthermore, due to the …
Palbociclib Suppresses The Cancer Stem Cell Properties And Cell Proliferation Through Increased Levels Of Mir-506 Or Mir-150 In Panc-1 And Miapaca-2 Cells, Özge Rencüzoğullari, Eli̇f Damla Arisan
Palbociclib Suppresses The Cancer Stem Cell Properties And Cell Proliferation Through Increased Levels Of Mir-506 Or Mir-150 In Panc-1 And Miapaca-2 Cells, Özge Rencüzoğullari, Eli̇f Damla Arisan
Turkish Journal of Biology
The prognostic characteristics of pancreatic cancer (PC) are determined by the contributing factors from the tumor microenvironment. Leptin is a critical oncogenic factor released by adipocytes as an adipokine into the tumor microenvironment, where it promotes tumor development by activating cancer stem cell (CSC) molecular regulators Notch, Hedgehog, and Wnt/ß-catenin signaling. One of the downstream targets of these pathways is CDK4/6 and cyclin D which is controlled by P16 INK4A that is highly mutated in PC. Therefore, the purpose of this study was to determine the effect of a CDK4/6 inhibitor, palbociclib, on Leptin-induced PC cells and to target the …
Three-Dimensional Mesenchymal Stem Cell Laden Scaffold Of Icariin Sustained-Release For Bone Regeneration, Yanbing Liu, Yan Fang
Three-Dimensional Mesenchymal Stem Cell Laden Scaffold Of Icariin Sustained-Release For Bone Regeneration, Yanbing Liu, Yan Fang
Turkish Journal of Biology
Background/aim: Icariin (ICA)-loaded zein/PLGA nanofiber membrane combined with MSCs was prepared by coaxial electrospinning and bioelectrospraying. Materials and methods: SEM and TEM were used to evaluate the surface morphology and microstructure of the fiber membrane. Ultraviolet spectrophotometry was used to detect drug release. A LIVE/DEAD Viability/Cytotoxicity Kit and fluorescence staining were used to detect cell morphology and activity. Alkaline phosphatase and calcium mineralization deposition were used to evaluate the osteoinductive activity of the scaffold. Dynamic mechanical analysis was used to determine the Young's modulus, maximum load, and maximum elongation of the prepared scaffold. Western blot was used to detect the …
Gemcitabine In Combination With Epibrassinolide Enhanced The Apoptotic Response In An Er Stress-Dependent Manner And Reduced The Epithelial-Mesenchymal Transition In Pancreatic Cancer Cells, Pinar Obakan Yerli̇kaya, Leila Mehdizadehtapeh, Özge Rencüzoğullari, Fadina Kuryayeva, Sena Sedef Çevi̇kli̇, Şevval Özağar, Si̇bel Pinar Odabaş, Sude Tunçkol, Hakan Yeti̇m, Ajda Çoker Gürkan, Eli̇f Damla Arisan
Gemcitabine In Combination With Epibrassinolide Enhanced The Apoptotic Response In An Er Stress-Dependent Manner And Reduced The Epithelial-Mesenchymal Transition In Pancreatic Cancer Cells, Pinar Obakan Yerli̇kaya, Leila Mehdizadehtapeh, Özge Rencüzoğullari, Fadina Kuryayeva, Sena Sedef Çevi̇kli̇, Şevval Özağar, Si̇bel Pinar Odabaş, Sude Tunçkol, Hakan Yeti̇m, Ajda Çoker Gürkan, Eli̇f Damla Arisan
Turkish Journal of Biology
Gemcitabine is a broad-spectrum antimetabolite and a deoxycytidine analog recognized as a standard therapy alone or in combination with other antineoplastic agents in the therapy of pancreas cancer. Drug resistance following gemcitabine treatment is a common phenomenon; therefore, combinational therapy models are usually preferred. Pancreatic ductal adenocarcinoma, or pancreas cancer, is the fourth leading cause of cancer-related deaths worldwide. With the increasing incidence of pancreatic cancer every year, the mortality rate is also rising significantly because of late diagnosis, and limited chemotherapy options. Adjuvant chemotherapy after surgical resection is the typical option for the treatment of early pancreatic cancer. Mostly, …
Supplement : Abstract Proceedings Of The 7th International Congress Of The Molecular Biology Association Of Turkey
Turkish Journal of Biology
No abstract provided.
Doxorubicin-Induced Transcriptome Meets Interactome: Identification Of New Drug Targets, Hi̇lal Taymaz-Ni̇kerel
Doxorubicin-Induced Transcriptome Meets Interactome: Identification Of New Drug Targets, Hi̇lal Taymaz-Ni̇kerel
Turkish Journal of Biology
The working mechanism of the chemotherapeutic drug doxorubicin, which is frequently used in cancer treatment, its effects on cell metabolism, and pathways activated solely by doxorubicin are not fully known. Understanding these principles is important both in improving existing therapies and in finding new drug targets. Here, I describe a systems-biology approach to find a generalizable working principle for doxorubicin by superimposition of human interactome over gene datasets commonly expressed among various cancer types. The common ?in at least two different diseases?transcriptional response of distinctive cancer cell lines to doxorubicin was reflected via 199 significantly and differentially expressed genes, mostly …