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Articles 1471 - 1500 of 1681
Full-Text Articles in Biology
Molecular Characterization Of The Gene Encoding For The Salivaricin B Activity And Its Flanking Sequences, Osman Çataloluk
Molecular Characterization Of The Gene Encoding For The Salivaricin B Activity And Its Flanking Sequences, Osman Çataloluk
Turkish Journal of Biology
Lactic acid bacteria (LAB) produce many different antimicrobial proteins, some of which have potential in food preservation. The molecular analysis of bacteriocins has gained much attention and has advanced rapidly in recent years, and it became routine to analyze the way in which bacteriocins are expressed and translocated. Since they are associated with foods of plant and animal origins, the shared interest is that they can be utilized as a vector to deliver the active constituents at the target in the gastrointestinal tract after digestion and be used in therapy. In this respect, the molecular pattern of the expression and …
In Situ Localization Of Germin Gene Expression During Auxin Induced Callus Formation, Mahmut Çalişkan
In Situ Localization Of Germin Gene Expression During Auxin Induced Callus Formation, Mahmut Çalişkan
Turkish Journal of Biology
Wheat embryogenic callus was induced by treating immature wheat embryos with 2,4-D. Digoxigenin-labeled germin sense and anti-sense RNA probes were prepared by in vitro transcription. By using these probes it was revealed that there was a striking induction of germin gene expression prior to visible callus formation. Although no germin gene expression was localized on the sections of immature embryos which were not incubated in callus induction medium, following 4 hours incubation in callus induction medium, germin mRNA was localized on the sections of immature embryos. Germin gene expression more conspicuous and widespread on the sections which were incubated for …
Restriction Fragment Length Polymorphism Analysis (Rflp) Of Some Streptomyces Strains From Soil, Ekrem Atalan
Restriction Fragment Length Polymorphism Analysis (Rflp) Of Some Streptomyces Strains From Soil, Ekrem Atalan
Turkish Journal of Biology
To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis. A dendrogram were constructed using the unweighed pair group method using arithmetic averages algorithm (UPGMA) after analysis restriction patterns. Five RFLP groups were obtained anone test strain was left as a single member group. RFLP profile indicated that unknown strains could be identified with data based on the interspacer region of …
Pulsed-Field Gel Electrophoresis (Pfge) Technique And Its Use In Molecular Biology, Esi̇n Basim, Hüseyi̇n Basim
Pulsed-Field Gel Electrophoresis (Pfge) Technique And Its Use In Molecular Biology, Esi̇n Basim, Hüseyi̇n Basim
Turkish Journal of Biology
In recent years, the use of pulsed-field gel electrophoresis (PFGE) in the molecular biology area has been subject to much research. PFGE is a powerful tool for characterizing various strains at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria that are poorly understood at the genetic level as well as in separating chromosomes in microorganisms, and in the long-range mapping of mammalian genes. PFGE also has advantage of examining the elongated and oriented configuration of large DNA molecules in agarose gels at finite field strengths. In this …
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Viral Control Of The European Pine Sawfly, Neodiprion Sertifer (Geoffroy) In Turkey, Mustafa Yaman, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
The European pine sawfly, Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae), is a serious pest of pine plantations and ornamentals. Chemical pesticides used to control this pest have a detrimental effect on the environment. The Neodiprion sertifer nuclear polyhedrosis virus (NsNPV) frequently causes considerable decreases in the populations but doses not harm other animals or plants. In this study, we tested five different concentrations of NsNPV against the larvae of N. sertifer and showed viral infection using Giemsa staining. When a viral concentration of 5x10^9 PIB/ml (Polyhedral Inclusion Body) was utilized, larval mortality started on the fourth day and reached 90.4% on …
Callus Induction And Plant Regeneration From Mature Embryos Of Oat (Avena Sativa L.), Melahat A.Bi̇rsi̇n, Sertaç Önde, Murat Özgen
Callus Induction And Plant Regeneration From Mature Embryos Of Oat (Avena Sativa L.), Melahat A.Bi̇rsi̇n, Sertaç Önde, Murat Özgen
Turkish Journal of Biology
The aim of this study, which was conducted in Ankara University, Faculty of Agriculture, Department of Field Crops, biotechnology laboratory in 1998-1999, was to determine the callus induction and plant regeneration capacity of oat (Avena sativa L.). The mature embryos of 10 oat genotypes were utilised for callus induction. The statistical analysis of the results revealed that callus induction and plant regeneration capacity were dependent on the oat genotype. However, a significant relationship was not established between callus induction and regeneration capacity and, furthermore, the number of regenerated plants was determined to be directly related to the callus production capacity.
Susceptibility Of Different Bean (Phaseolus Vulgaris L.) Cultivars To Agrobacterium Tumefaciens, Azi̇z Karakaya, Sebahatti̇n Özcan
Susceptibility Of Different Bean (Phaseolus Vulgaris L.) Cultivars To Agrobacterium Tumefaciens, Azi̇z Karakaya, Sebahatti̇n Özcan
Turkish Journal of Biology
Although several direct gene transfer techniques have been developed, Agrobacterium tumefaciens is still preferred for the transfer of foreign genes into many plant species. Perhaps the greatest weakness of A. tumefaciens is the host range limitation. Therefore, in this study, the susceptibility of different bean cultivars to oncogenic A. tumefaciens strain A281 was assessed. The cultivars 4F-2409, Karacaşehir-90, Akman-98 and Eskişehir-855 resulted in higher frequencies of tumor formation than the other cultivars tested. Tumor formation was not observed in Afyon-3, IVD-10, Göynük-98, Yunus-90 or Şehirali-90 bean cultivars. Tumor formation was observed on both the hypocotyl and epicotyl portions of the …
Ga3, Aba And Cytokinin Production By Lentinus Tigrinus And Laetiporus Sulphureus Fungi Cultured In The Medium Of Olive Oil Mill Waste, Bi̇rgül Özcan, S. Fati̇h Topcuoğlu
Ga3, Aba And Cytokinin Production By Lentinus Tigrinus And Laetiporus Sulphureus Fungi Cultured In The Medium Of Olive Oil Mill Waste, Bi̇rgül Özcan, S. Fati̇h Topcuoğlu
Turkish Journal of Biology
The levels of endogenous form of free, bound and total-gibberellic acid (GA_3), abscisic acid (ABA) and cytokinin (zeatin) in culture medium were determined. The changes in dry weight of the mycelium, dependent on the culture periods, was examined through the use of white-rot fungus Lentinus tigrinus and brown-rot fungus Laetiporus sulphureus which were cultured in the medium of olive oil mill waste in a static culture. Spectrophotometric techniques were used to determine the amounts of endogenous GA_3, ABA and zeatin. As a result, fungi used in this study showed that they synthesized GA_3, ABA and zeatin as a primary or …
The Evaluation Of Alkalitolerant-Mesophilic Streptomyces By Gas Chromatography And Pyrolysis Mass Spectrometry, Nevzat Şahi̇n, Kami̇l Işik, Ergi̇n Kari̇ptaş, Michael Goodfellow
The Evaluation Of Alkalitolerant-Mesophilic Streptomyces By Gas Chromatography And Pyrolysis Mass Spectrometry, Nevzat Şahi̇n, Kami̇l Işik, Ergi̇n Kari̇ptaş, Michael Goodfellow
Turkish Journal of Biology
Thirty-eight alkalitolerant-mesophilic Streptomyces, defined by Saddler in his numerical taxonomic study, were evaluated by gas chromotography and pyrolysis mass spectrometry. Group I Streptomyces sp. AK409 and group II Streptomyces sp. AK029 selected duplicated strains were clustered in their own groups. It was observed that the results of gas chromatography and pyrolysis mass spectrometry was in good congruance with the numerical taxonomic results. Therefore, it can be concluded that the different chemotaxonomic methods provide rapid and reproducible information for classifying biotechnologically significant Streptomyces strains.
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
In Vitro Model For The Study Of Listeria And Salmonella Adherence To Intestinal Epithelial Cells, Nevzat Yurdusev
Turkish Journal of Biology
A quantitative test to study Listeria and Salmonella adherence to epithelial cells was developed. The quantitative test is rapid and allows the simultaneous testing of many variables such as the adherence ability of different bacteria to their target cells as well as the capability of various molecules to inhibit bacterial adherence. By using a strain-specific standard curve in each test, the test for the quantification of adherent bacteria became specifically sensitive. Non-viable biotinylated bacteria and immobilized cells or their extract were found relevant for the study of bacteria-epithelial cell interactions. Adherence ability of Listeria strains was found to be 10-fold …
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
The Cytogenetical Comparisons Of Spalax (Rodentia: Spalacidae) Populations From Middle Kızılırmak Basin, Turkey, Eşref Yüksel, Mehmet Doğan Gülkaç
Turkish Journal of Biology
Chromosomal polymorphism and speciation problem in genus Spalax has been investigated. All the specimens were identified as Spalax leucodon cilicicus Mehely, 1909. In according to karyotype analysis, two different populations were described from the field. The diploid number of Population A from south ant northwest of the field were found to be 60 and number of fundamental numbers were 80. The corresponding numbers in the Population B from northeast of the field were 54 and 74 respectively. In the result, the possibility of inversions in the Population B was discussed from evolutionary point of view and also the evolutionary background …
Availability Of Use Of Total Extracellular Proteins In Sds-Page For Typing Staphylococcus Aureus And Coagulase-Negative Staphylococci, Sefa Can Saçilik, Özlem Osmanağaoğlu, A. Hakki Sayar, Cumhur Çökmüş
Availability Of Use Of Total Extracellular Proteins In Sds-Page For Typing Staphylococcus Aureus And Coagulase-Negative Staphylococci, Sefa Can Saçilik, Özlem Osmanağaoğlu, A. Hakki Sayar, Cumhur Çökmüş
Turkish Journal of Biology
A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. …
Determination Of Some Fungal Metabolite As Influenced By Temperature, Time, Ph And Sugars By Bioassay Method, Meti̇n Diğrak, Sami̇ Özçeli̇k
Determination Of Some Fungal Metabolite As Influenced By Temperature, Time, Ph And Sugars By Bioassay Method, Meti̇n Diğrak, Sami̇ Özçeli̇k
Turkish Journal of Biology
This study investigated the fungal biomass and inhibition of Bacillus megaterium DSM 32 with extracts of Fusarium equiseti, Penicillium italicum, Penicillium digitatum, Aspergillus candidus and Aspergillus parasiticus in modified Czapek Dox Broth (mCDB) as affected by temperature (6, 15, 25 °C), incubation time (up to 4 wk), pH (3.0, 5.0 and 8.0) and glucose the and fructose (4.0, 6.0 and 9.0%). The biomass production was greatest at 25 °C. In general, biomass production was greatest in media containing an addition of 4.0% glucose or 6.0% fructose; however, bacterial inhibition production was reduced by elevated sugars in mCDB over a 4-wk …
Effects Of Cd^{2+}, Zn^{2+}, Li^+ And Al^{3+} On The Fertilization-Induced Membrane Potential Changes In The Egg Of The Frog Rana Cameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Effects Of Cd^{2+}, Zn^{2+}, Li^+ And Al^{3+} On The Fertilization-Induced Membrane Potential Changes In The Egg Of The Frog Rana Cameranoi, Şeref Erdoğan, Gülay Loğoğlu, Kerem Tuncay Özgünen, Tuncay Özgünen
Turkish Journal of Biology
The apparent change in the egg membrane potential at fertilization gives rise to fertilization potential (FP). FP is the initial, transient electrical block to polyspermy as shown in most species especially those exhibiting external fertilization, and it protects the egg from a second sperm entry until the permanent, mechanical block is set up. Polyspermy risk is increased when this effective electrical block cannot reach a certain peak value or when it is of short duration. On the other hand, metal accumulation in the external environment is potentially toxic to living beings, and we aimed in the present study to evaluate …
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Assessment Of Polymorphic Aflp Markers In Triticum Durum And Aegilops Sp., Aytül İnci̇rli̇, Hati̇ce Bi̇lgi̇ç, Mahi̇nur S. Akkaya
Turkish Journal of Biology
Compared to other Polymerase Chain Reaction (PCR) based molecular markers, the Amplified Fragment Length Polymorphism (AFLP) marker system probably requires more technical expertise for the sequential enzymatic reaction steps. In addition, the reproducibility of the AFLP marker patterns is much more dependent on the quality and the purity of the DNA samples to be processed. The present study describes the optimization of reaction conditions and determination of the most polymorphic selective amplification primer sets in a comparison of winter and spring type Turkish durum wheat varieties. AFLP products resulted in about 100 bands, and the number of polymorphic AFLP loci …
In Vitro Inhibition Of The Mycelial Growth Of Some Root Rot Fungi By Rhizobium Leguminosarum Biovar Phaseoli Isolates, İbrahi̇m Özkoç, Muhammet Hani̇fi̇ Deli̇veli̇
In Vitro Inhibition Of The Mycelial Growth Of Some Root Rot Fungi By Rhizobium Leguminosarum Biovar Phaseoli Isolates, İbrahi̇m Özkoç, Muhammet Hani̇fi̇ Deli̇veli̇
Turkish Journal of Biology
In solid medium, the effects of twenty-three Rhizobium leguminosarum biovar phaseoli isolates on the mycelium development of the phytopathogenic fungi of three different genera (Fusarium oxysporum, Pythium ultimum and Rhizoctonia solani) were tested in vitro. The experiments were achieved by applying the superimpose dual culture technique. Most of the rhizobia tested inhibited the fungal development. The inhibition rates displayed differences in accordance with the bacteria and fungi isolates utilized. However, the inhibitory effect of the rhizobia occurred most on the isolates of Fusarium and Pythium species (up to 30.35% and 29.75% respectively) and least on those of Rhizoctonia species (16.03%). …
The Cytogenetic Effects Of Npyr On Mice, Mehmet Korkmaz, Ahmet Çolak
The Cytogenetic Effects Of Npyr On Mice, Mehmet Korkmaz, Ahmet Çolak
Turkish Journal of Biology
The effects of N-nitrosopyrrolidine (NPYR) on mice chromosomes and micronucleus induction were investigated in order to evaluate its genotoxic effects. Twenty 10-12 week old mice and 40 8- 10 week old male mice ( Mus musculus var. albinos) were used for chromosome analysis without sex discrimination and micronucleus tests respectively. Mice were intraperitoneally treated in single injections with three dose levels (100, 200 and 300 mg/kg) of NPYR with isotonic saline as the solvent vehicle. Bone marrow was sampled at 24 and 48 h postinjection. Examination of the mice bone marrow chromosomes at the metaphase (24 h) showed that in …
A Quantitative And Qualitative Study On The Phytoplankton Of Palandöken Pond, Hasan Gürbüz, Zekeri̇ya Altuner
A Quantitative And Qualitative Study On The Phytoplankton Of Palandöken Pond, Hasan Gürbüz, Zekeri̇ya Altuner
Turkish Journal of Biology
In this research, the phytoplankton community of Palandöken pond was investigated quantitatively and qualitatively in samples taken vertically from the surface to 10 m depth between October 1990 and October 1992. The physical and chemical properties of the pond water were also determined. 99 taxa were determined in the phytoplankton and the proportions of them were as follows: Bacillariophyta 79%, Chlorophyta 10%, Cynophyta 6%, Euglenophyta 5%. The phytoplankton generally increased in the spring and early fall whereas in the summer and late fall it decreased. Physical factors had the greatest effect on phytoplankton growth.
The Monthly Variations Of Blood Glucose Level Of Capoeta Tinca Capoeta(Heckel, 1843) In Çoruh Basin-Oltu Stream, Ayhan Yildirim, Mustafa Türkmen, İrfan Altuntaş
The Monthly Variations Of Blood Glucose Level Of Capoeta Tinca Capoeta(Heckel, 1843) In Çoruh Basin-Oltu Stream, Ayhan Yildirim, Mustafa Türkmen, İrfan Altuntaş
Turkish Journal of Biology
In this study, the monthly variation of blood glucose level of Capoeta tinca caught from in Çoruh Basin - Oltu Stream, between Augist 1995 and June 1996 was determined. It was determined that the average blood glucose level was 86.19±3.86 mg/dl in males and 83.58±3.77 mg/dl in females. The blood glucose level was highest (116.36±8.58 mg/dl) in April, and lowest (51.00±7.10 mg/dl) in June.
Detection Of Mutagenic Effects Of 2, 4, 5 Tri (Sübstitüe) Phenyl Imidazole And Its Derivatives In Ames/Salmonella/Test System, Ayşe Mercangöz, Berri̇n Ayaz Tüylü
Detection Of Mutagenic Effects Of 2, 4, 5 Tri (Sübstitüe) Phenyl Imidazole And Its Derivatives In Ames/Salmonella/Test System, Ayşe Mercangöz, Berri̇n Ayaz Tüylü
Turkish Journal of Biology
The mutagenic effects of 2, 4, 5 tri-phenylimidazole and its nine different derivatives, which are aimed at producing medicine as a primary substance, were investigated by the Ames Salmonella/Microsome plate assay. The test compounds were directly effective, especially in the TA 98 strain. A relationship was found between the mutagenic effect and the positions of the methyl and methoxy groups.
The Inhibition Effect Of Yoghurt Starter Culture Metabolites, Belma Aslim, Yavuz Beyatli, Kadi̇r Halkman
The Inhibition Effect Of Yoghurt Starter Culture Metabolites, Belma Aslim, Yavuz Beyatli, Kadi̇r Halkman
Turkish Journal of Biology
In this study effect of some separate and combined metabolic compounds produced by lactic acid were tested on food pathogenic and contaminant microorgnasms. For this study, five strains of each Lactobacillus bulgaricus and Streptococcus thermophilus were used. The inhibition effects of the metabolic products (lactic acid, hydrogen peroxide, acetaldehyde, diacetyl) produced by the stains on test bacteria ( Staphylococcus aureus, Escherichia coli) were determined. Also, a random combinations of the strains were prepared and the metabolic compounds produced by these cultures and their antimicrobial activities on tested bacteria were determined. By regarding the metabolic products mainly, the samples of lactic …
The Effects Of Malt Extract And Corn Strach On The Vegetativedevelopment And Morphology Of The Pleurotus Floridafovose Mycelia, Abdunnasir Yildiz
The Effects Of Malt Extract And Corn Strach On The Vegetativedevelopment And Morphology Of The Pleurotus Floridafovose Mycelia, Abdunnasir Yildiz
Turkish Journal of Biology
No statistical differences were determined in the effects of malt extract (ME) and corn strach (CS) doses on the vegetative development of mycelia (P>0.05). The mycelia in all gropus covered 9.0 cm agar plates in 9.0 days and some characteristic differences, development from (vertical or horizontal), cluster formation, tickening and tissue formations, were observed. In the group containing 2% CS, the mycelia formed a sparse development horizontally on the surface and center of the agar plate, whereas clustering, thickening and tissue formation wereobserved in the form of a thick zone on the edges of the petri dishes. In 1.5% …
Changes In Liver Cytoplasmic Glutathione-S-Transferase And Microsomalnitrosodimethylamine Demethylase Activities In Chicks Obtainedfollowing Daminozide Treatment, Yavuz Si̇li̇ğ, V. Kenan Çeli̇k, Ati̇lla Atalay
Changes In Liver Cytoplasmic Glutathione-S-Transferase And Microsomalnitrosodimethylamine Demethylase Activities In Chicks Obtainedfollowing Daminozide Treatment, Yavuz Si̇li̇ğ, V. Kenan Çeli̇k, Ati̇lla Atalay
Turkish Journal of Biology
In this study, microsomal nitrosodimethylamine demethylase (NDMAd) and cytoplasmic glutathione-s-tarnsferase (GST) activities in the livers of chicks obtained from the LD 10 (3 mg/ml) and LD 30 (9 mg/ml) doses of daminozide (Succinic acid 2,2-Dimethylhydrazide) injected Ross-Pm-3 fertilized chicken eggs were investigated. Spectrophotometric determination of NDMAd activity was performed at 412 nm by the detection of yellow coloured compound formed as a product of the reaction of formaldehyde with Nash reagent. No significant increase was observed in NDMAd activity (P>0.05) of the group treated with LD 10 dose although NDMAd activity was found to be significantly increased (P
The Effect Of Olive Oil Mill Effluent On The Mitotic Cell Division And Total Protein Amount Of The Root Tips Of Triticum Aestivuml., Mehmet Aybeke, Göksel Olgun, Uğur Sidal, Dürdane Kolankaya
The Effect Of Olive Oil Mill Effluent On The Mitotic Cell Division And Total Protein Amount Of The Root Tips Of Triticum Aestivuml., Mehmet Aybeke, Göksel Olgun, Uğur Sidal, Dürdane Kolankaya
Turkish Journal of Biology
In this work sitotoxic and mutagenic effects Olive Oil Mill Effluent (OOME) on the root tips of Triticum aestivumL. were investigated. In this purpose, germination rate of seeds, mitotic division abnormalities and total protein amounts were evaluated. The seeds kept in various OOME concentrastions, it was determinated that germination rate decreased, whilst mitotic abnormalities and mitotic cell division frequency increased. Especially, the increased cell division frequency was of significance, almost several times higher than the control group. The cells having multinucleus or fragmented nucleus and numerical or sutructural mutations were also found. Furthermore it was observed that total protein amounts …
Antioxidant Enzymes Activities In Chemically Warfared Samples Ofdociostaurus Maroccanusobtained From An Epidemic Population, Aysun Özkan, Gülgün Gündüz, Battal Çiplak, Kayahan Fişkin
Antioxidant Enzymes Activities In Chemically Warfared Samples Ofdociostaurus Maroccanusobtained From An Epidemic Population, Aysun Özkan, Gülgün Gündüz, Battal Çiplak, Kayahan Fişkin
Turkish Journal of Biology
Antioxidant enzyme activities at first, second, third nymphal stage and adult individuals of nition [O-O-dimethyl-O-(3-methyl-4-nitro-phenyl) phosphorothioate] administered D. maroccanusare investigated. Living specimens where nition had been administered for chemical contention by Agricultural Head Office of Antalya Province in 1996 were taken from epidemic population of Eynif plain (Ibradi, Antalya). Catalase, GSH-reductase, GSH-peroxidase and Se-dependent-GSH-peroxidase activities are assayed spectorofotometrically. The catalase activity did indicate no significant change in any nymphal stage. GSH-reductase activity increased in adult individuals. Se-GSH-peroxidase activity decreased with second nymphal stage and increased with the third one, Whereas GSH-peroxidase activity increased at second nymphal stage of D. maroccanus.
The Effect Of Some Nutrients Supplementation To Synthetic Compostwith Wheat Straw Before Mycel Inoculation, On Mycelium Growth, Yieldand Early Ripe Of Agaricus Bitorquis(Quél). Sacc, Hasan Hüseyi̇n Doğan, Celaleddi̇n Öztürk, Cengi̇z Akköz
The Effect Of Some Nutrients Supplementation To Synthetic Compostwith Wheat Straw Before Mycel Inoculation, On Mycelium Growth, Yieldand Early Ripe Of Agaricus Bitorquis(Quél). Sacc, Hasan Hüseyi̇n Doğan, Celaleddi̇n Öztürk, Cengi̇z Akköz
Turkish Journal of Biology
The effect of some nutrients added to synthetic compost with wheat straw, on mycelium growth, yield and early ripe of Agaricus bitorquis (Quél.) Sacc. were investigated. The compost was prepared by fermentation and chemical disinfection methods. Corn flour, wheat flour, hen grain, soybean meal and sunflower seed hulls were used as additional nutrients. The experiments were made at the temperatüre 20-22°C and 80-90% relative humidity in the yield rooms. The shortest mycelium growth period (average 14 days) were determined in containing 350 g/10 kg and 450 g/10 kg sunflower seed hulls. The maximum production were taken in the sacks of …
Effects Of Gibberellic Acid On Male Sterility, Seed Yield And Oil And Fatty Acid Syntheses Of Safflower ( Carthamus Tinctorius L.), Hasan Baydar
Turkish Journal of Biology
The purpose of this study was to determine the effects of gibberellic acid (GA 3 ) on male and female sterility, seed yield, oil and fatty acid syntheses, growth and development properties in safflower ( Carthamus tinctorius var. Yenice). GA 3 was applied in five different doses at three different stages, and induced male sterility at a rate of up to 93.0%. This finding has important implications for the practical use of GA 3 in hybrid seed production. GA 3 applications significantly decreased the seed yield per plant both in isolation and non-isolation. Although fatty acid syntheses did not change …
The Use Of Random Amplified Polymorphic Dna(Rapd) Markers In Sex Discrimination In Niletilapia, Oreochromis Niloticus (Pisces: Cichlidae), Fevzi̇ Bardakci
The Use Of Random Amplified Polymorphic Dna(Rapd) Markers In Sex Discrimination In Niletilapia, Oreochromis Niloticus (Pisces: Cichlidae), Fevzi̇ Bardakci
Turkish Journal of Biology
Random amplified polymorphic DNA (RAPD) markers were successfully used in discrimination of sexes in Nile tilapia fish ( Oreochromis niloticus) using linear discriminant function analysis. The results provide support for the view that major genetical sex determining factors exist in tilapia.
Morphological Changes In Xenopus Laevisembryoswith Lithium And Evaluation Of The Fetax Test, Ayper Boğa, Seçi̇l Bi̇nokay, Ayşe Kendi̇rci̇, Tuncay Özgünen, Emi̇ne Kizilkanat
Morphological Changes In Xenopus Laevisembryoswith Lithium And Evaluation Of The Fetax Test, Ayper Boğa, Seçi̇l Bi̇nokay, Ayşe Kendi̇rci̇, Tuncay Özgünen, Emi̇ne Kizilkanat
Turkish Journal of Biology
The main goal was to standardize the FETAX (Frog Embryos Teratogenesis Assay: Xenopus) test. Since lithium ions are known to be teratogenic for embryos of many organisms, they were prefferred for our first standardization assay. Xenopus laevis embryos were exposed to lithium chloride (from 2x10 -4 to 6 x 10 -3 M concentrations) for 96 hours at 2-4 cell stages of development. Exposure to lower LiCl concentrations permitted larvae to survive and to develop into normal ones. On the other hand, depending on the increase in the LiCl concentration, the body length and survival rate (%) decreased. In addition, the …
Simple Production And Purification Of Monoclonalantibodies In Serum-Free Medium, Aynur Başalp, Beyazit Çirakoğlu, Engin Bermek
Simple Production And Purification Of Monoclonalantibodies In Serum-Free Medium, Aynur Başalp, Beyazit Çirakoğlu, Engin Bermek
Turkish Journal of Biology
Although serum is a main source of essential nutrients and growth factors, its use creates highly complex and poorly defined media. The use of serum-free media is more efficient than the use of media containing serum especially when the purification and characterization of antibodies are considered. In this study, we developed a serum-free medium system for cultivation of hybrid cells producing monoclonal antibodies specific for Tobacco Mosaic Virus (1F5) and 17\beta-estradiol (10F4) (15D4). Cells were cultivated in a medium with 10 % FCS for three days, and subsequently in serum-free medium for two days. The procedure was repeated once again …