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Articles 1411 - 1440 of 1681
Full-Text Articles in Biology
Alkaliphilic Micro-Organisms And Habitats, Zeynep Ulukanli, Meti̇n Diğrak
Alkaliphilic Micro-Organisms And Habitats, Zeynep Ulukanli, Meti̇n Diğrak
Turkish Journal of Biology
Alkaline environments are typical extreme environments which include naturally occurring soda lakes, deserts, soils and artificially occurring industrial-derived waters. Micro-organisms that occupy extreme pH environments have resulted in the definition of an unusual group, termed alkaliphiles. In this review, the current status of the biodiversity of alkaliphilic micro-organisms in various environments and aspects of their biotechnological potential are summarised briefly.
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Turkish Journal of Biology
A soluble lectin was purified from the hemolymph of Agrotis segetum by two methods, via affinity chromatography on a Sepharose-4B column and gel filtration on a Superdex-200 column. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2-Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex-200. Electron microscopic observation of the purified lectin was reticular in structure and consisted of tandem aligned spherical basic units.
Effects Of Subchronic Treatment Of Some Plant Growth Regulators On Serum Enzyme Levels In Rats, İsmai̇l Çeli̇k, Hanefi̇ Özbek, Yasi̇n Tülüce
Effects Of Subchronic Treatment Of Some Plant Growth Regulators On Serum Enzyme Levels In Rats, İsmai̇l Çeli̇k, Hanefi̇ Özbek, Yasi̇n Tülüce
Turkish Journal of Biology
The effects of a sublethal concentration of three plant growth regulators (PRGs) on serum enzymes in rats were investigated under laboratory conditions. 100-ppm of PRGs, indoleacetic acid (IAA), indolebutiric acid (IBA) and kinetin were administered orally to 8 rats ad libitum for 3 weeks. The hormon treatments caused different effects on the level of serum alanin aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), amylase and creatine phosphokinase (CPK) in comparison to those of control rats. According to the results, while the levels of LDH and CPK were increased significantly by IBA, the levels of AST, LDH and CPK were …
The Chronic Effects Of Teucrium Polium On Some Blood Parameters And Histopathology Of Liver And Kidney In The Rat, Khaled Khleifat, Jumah Shakhanbeh, Khaled A. Tarawneh
The Chronic Effects Of Teucrium Polium On Some Blood Parameters And Histopathology Of Liver And Kidney In The Rat, Khaled Khleifat, Jumah Shakhanbeh, Khaled A. Tarawneh
Turkish Journal of Biology
The effects of chronic treatment with small sublethal doses (20 mg/kg and 50 mg/kg) of Teucrium polium ethanolic extract were investigated on the hematological and biochemical composition of the blood, histopathology of the liver and kidney, and fertility in the rat. Hematological and biochemical parameters of the blood, as well as sperm count, morphology and motility were normal after 6 weeks of herbal treatment. However, blood urea (P < 0.05) and cholesterol (P < 0.005) were significantly increased, with marked cytoplasmic vacuolation of the liver and kidney cells after chronic treatment with 50 mg/kg of the plant. These results demonstrate some histopathological effects of T. polium on the liver and kidney under long-term administration conditions.
A New Molecular Technique For The Identification Of Micro-Organisms In Biological Treatment Plants: Fluorescent In Situ Hybridization, Gülnur Coşkuner
A New Molecular Technique For The Identification Of Micro-Organisms In Biological Treatment Plants: Fluorescent In Situ Hybridization, Gülnur Coşkuner
Turkish Journal of Biology
Wastewater treatment has gained increased importance worldwide due to increasing demand on fresh clean water supplies. Biological treatment achieved by the activities of micro-organisms, is one of the most widely used processes in the treatment of wastewater. High numbers and activities of some groups of micro-organisms are required to treat wastewater in biological processes. In contrast, some groups of micro-organisms must be absent from the system since their presence and activities cause problems in the treatment of wastewater. The structure and function of micro-organisms in wastewater treatment plants (WWTP) have been investigated for decades. However, the identification of both the …
Effects Of Photoperiod And Temperature On Growth And Reproductive Organ Mass In Adult Male Mongolian Gerbils, Meriones Unguiculatus, Bülent Gündüz
Effects Of Photoperiod And Temperature On Growth And Reproductive Organ Mass In Adult Male Mongolian Gerbils, Meriones Unguiculatus, Bülent Gündüz
Turkish Journal of Biology
Rates of growth and sexual maturation of Mongolian gerbils vary in response to photoperiod. Previous work has shown that the responses of the testes and body weight of juvenile and adult gerbils are influenced not only by the photoperiods but also by other factors. The present work sought to determine if Mongolian gerbils alter body mass, body composition and/or reproductive organ mass in response to different photoperiods and temperatures. Male Mongolian gerbils were raised under long (14L) or short (10L) photoperiods from 26 days of age. Photoperiods did not affect body mass and body length. Temperature affected body mass only …
Food Intake And Feed Conversion Ratios In Abant Trout (Salmo Trutta Abanticus T., 1954) And Rainbow Trout (Oncorhynchus Mykiss W., 1792) In Pond Culture, İrfan Uysal, Ati̇lla Alpbaz
Food Intake And Feed Conversion Ratios In Abant Trout (Salmo Trutta Abanticus T., 1954) And Rainbow Trout (Oncorhynchus Mykiss W., 1792) In Pond Culture, İrfan Uysal, Ati̇lla Alpbaz
Turkish Journal of Biology
This study was carried out at DSI-Gölköy Fish Production Station in Bolu and lasted for 350 days. Food consumption (FC) and feed conversion ratios (FCR) in Abant and rainbow trout in pond culture were compared. Abant trout larvae obtained from eggs of wild Abant trout broodstocks in Lake Abant and rainbow trout larvae obtained from eggs of the cultured broodstocks in Gölköy Fish Production Station were used in this study. Trial I involved 4 groups (2 Abant and rainbow trout experimental groups and 2 Abant and rainbow trout parallel groups). Two hundred and thirty-six larvae were in each group; a …
Increasing The Injured Area On Hypocotyl Explants Of Flax (Linum Usitatissimum L.) Leads To High Frequency Callus-Based Shoot Regeneration, Mustafa Yildiz, Celal Er
Increasing The Injured Area On Hypocotyl Explants Of Flax (Linum Usitatissimum L.) Leads To High Frequency Callus-Based Shoot Regeneration, Mustafa Yildiz, Celal Er
Turkish Journal of Biology
In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as 0.8 g, 40%, 4.24 and 16.96, respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to 1.35 …
Effect Of Some Biocides On Glutathione-S-Transferase In Barley, Wheat, Lentil And Chickpea Plants, Gülseren Coşkun, Fi̇gen Zi̇hni̇oğlu
Effect Of Some Biocides On Glutathione-S-Transferase In Barley, Wheat, Lentil And Chickpea Plants, Gülseren Coşkun, Fi̇gen Zi̇hni̇oğlu
Turkish Journal of Biology
The conjugation of glutathione with herbicides and insecticides is considered to be one of the major detoxification mechanisms of plants. This conjugation is catalysed by a family of multifunctional proteins known as glutathione-s-transferases. Treatment of wheat (Triticum aestivum L. cv. Cumhuriyet-75), barley (Hordeum vulgare L. cv. Kaya), chickpea (Cicer arietinum L. cv. Ispanyol), and lentil (Lens culinaris Medik. cv. Kışlık-kırmızı-51) with the selective herbicides 2,4-D (2,4-Dichloro phenoxyacetic acid) and linuron (N-(3,4-dichlorophenyl)-N´-methoxy-N´-methyl urea), and insecticides parathion methyl (O,O-dimethyl O-(4-nitrophenyl) phosphorothioate) and malathion (S-1,2-bis(ethoxycarbonyl)ethyl O,O-dimethyl phosphorodithioate) caused a progressive increase in glutathione-s-transferase activity in roots and shoots. The maximum increase in enzyme …
The Effects Of Heavy Metals On Pollen Germination And Pollen Tube Length In The Tobacco Plant, A. Levent Tuna, Betül Bürün, İbrahi̇m Yokaş, Esi̇n Çoban
The Effects Of Heavy Metals On Pollen Germination And Pollen Tube Length In The Tobacco Plant, A. Levent Tuna, Betül Bürün, İbrahi̇m Yokaş, Esi̇n Çoban
Turkish Journal of Biology
This study was carried out to determine the effects of heavy metals (Ni, Fe, Pb, Co, Cd, Hg, Al, Zn and Cu) on pollen germination and pollen tube length in the tobacco plant (Nicotiana tabacum L.) cv. Karabağlar. The results showed that enhanced concentrations of heavy metals, except Fe, decreased the pollen germination rates and the pollen tube lengths. With Fe concentrations, on the other hand, first a positive, and then a negative relation was determined between the pollen characteristics examined. The most toxic effect on pollen germination was seen with the applications of Cu, Ni and Hg; on pollen …
Use Of Ram Horn Hydrolysate As Peptone For Bacterial Growth, Esabi̇ Başaran Kurbanoğlu, Ömer Faruk Algur
Use Of Ram Horn Hydrolysate As Peptone For Bacterial Growth, Esabi̇ Başaran Kurbanoğlu, Ömer Faruk Algur
Turkish Journal of Biology
Peptone from ram horn was compared with a casein and other peptones for bacterial growth. First, horns were ground and 35 g of horn flour was hydrolyzed chemically (acid hydrolysis). As a result of this process, 30 g of the 35 g horn flour (85.7%) could be hydrolyzed. Hydrolyzed material was completed to 400 ml with deionized water, and this resulting solution was termed ram horn hydrolysate (RHH). The contents of protein, nitrogen, ash, some minerals, total sugars, total lipids and amino acids of RHH were determined. It was found that it has both organic and inorganic materials sufficient for …
Assessment Of Two Highly Polymorphic Barley Microsatellite Markers For Detecting Polymorphism In Wheat, Emel Banu Büyükünal Bal, Mahi̇nur S. Akkaya
Assessment Of Two Highly Polymorphic Barley Microsatellite Markers For Detecting Polymorphism In Wheat, Emel Banu Büyükünal Bal, Mahi̇nur S. Akkaya
Turkish Journal of Biology
Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes. They are considered one of the most powerful molecular markers for many diverse applications in genome related studies, e.g., DNA fingerprinting for discrimination of genotypes and genetic mapping. Locus specificity, co-dominant inheritance and easy detection with PCR amplifications are some of the favorable features of this marker system. On the other hand, isolation of SSR markers depends on time-and labor-consuming processes. The need to obtain sequence information for locus …
Typing Of Some Clinically Significant Nocardia Strains Using A Digoxigenin-Labelled Rdna Gene Probe, Kami̇l Işik, Nevzat Şahi̇n, Ergi̇n Kari̇ptaş, Michael Goofellow
Typing Of Some Clinically Significant Nocardia Strains Using A Digoxigenin-Labelled Rdna Gene Probe, Kami̇l Işik, Nevzat Şahi̇n, Ergi̇n Kari̇ptaş, Michael Goofellow
Turkish Journal of Biology
In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically well-established species, except N. pseudobrasiliensis. Therefore, it can be concluded that …
Effects Of Dietary Carbohydrate Level On Gastric Evacuation And Return Of Appetite In The Rainbow Trout, Oncorhynchus Mykiss, Ahmet Adem Teki̇nay, Simon John Davies
Effects Of Dietary Carbohydrate Level On Gastric Evacuation And Return Of Appetite In The Rainbow Trout, Oncorhynchus Mykiss, Ahmet Adem Teki̇nay, Simon John Davies
Turkish Journal of Biology
Three experimental diets containing 14.9% (low carbohydrate; LC), 31.4% (medium carbohydrate; MC) and 42.3% (high carbohydrate; HC) of extruded wheat meal were fed to rainbow trout, Oncorhynchus mykiss, to investigate the significance of carbohydrate level on the rates of gastric evacuation and return of appetite (feed intake). Gastric evacuation measurements were performed by serial slaughter and recovery of digesta whilst X-radiography was used to estimate the rates of return of appetite. Gastric evacuation data of all three treatments were best explained by square root equations. The gastric evacuation rate of the LC group was significantly different (P0.05) between the emptying …
The Effect Of Different Explant Sources On Adventitious Shoot Regeneration In Flax (Linum Usitatissimum L.), Mustafa Yildiz, Sebahatti̇n Özcan, Celal Er
The Effect Of Different Explant Sources On Adventitious Shoot Regeneration In Flax (Linum Usitatissimum L.), Mustafa Yildiz, Sebahatti̇n Özcan, Celal Er
Turkish Journal of Biology
Hypocotyl and stem explants excised from in vitro and greenhouse-grown seedlings of Madaras, 1886 Sel. and Omega flax cultivars were compared in terms of percentage of explants producing shoots, shoot number per explant and total shoot number per petri dish. Hypocotyl explants resulted in better shoot regeneration. In vitro-grown seedlings were found to be more suitable than greenhouse-grown seedlings as an explant source.
Determination Of Some Properties Of Bacillus Isolated From Soil, Belma Aslim, Necdet Sağlam, Yavuz Beyatli
Determination Of Some Properties Of Bacillus Isolated From Soil, Belma Aslim, Necdet Sağlam, Yavuz Beyatli
Turkish Journal of Biology
Forty strains were isolated from six different soil samples. The soil samples were taken from the grasslands of Elmadağ, Çubuk, Gölbaşı, Haymana, Polatlı and Kazan near Ankara. As a result of the identification tests, 30 strains were identified as Bacillus subtilis, Bacillus megaterium, Bacillus firmus, Bacillus sphaericus, Bacillus thuringiensis, Bacillus pumilus and other Bacillus spp. The inhibitory effect of Bacillus strains against the test bacteria Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica and Micrococcus flavus was examined. It was found that B. thuringiensis D1, B. thuringiensis D3 and B. megaterium Y6 strains showed an inhibitory effect on all of the test …
Extractions Of Aspergillus Flavus Link Ex Gray And Cladosporium Cladosporioides (Fresen.) De Vries From Allergenic Microfungi And Application Of Toxicity Tests, Günay Çolakoğlu
Turkish Journal of Biology
Aspergillus flavus Link ex Gray and Cladosporium cladosporioides (Fresen.) de Vries, isolated from the soil of Florya Atatürk Forest, were extracted and some toxicity tests were carried out in order to determine their toxic effects on mice for the first time in Turkey. The method of extraction complied with the one described in the literature. During the process, Coca's solution was used as an extractive agent. Sterile filtration was employed in the sterilization process. The stocks were diluted to 1/10 of their concentrations before the toxicity tests were carried out. It was found that the A. flavus Link ex Gray …
Selective Isolation And Numerical Classification Of Novel Thermophilic Streptomycetes, Nevzat Şahi̇n, Erman Öztürk, Kami̇l Işik, Ergi̇n Kari̇ptaş, Reşi̇t Özkanca
Selective Isolation And Numerical Classification Of Novel Thermophilic Streptomycetes, Nevzat Şahi̇n, Erman Öztürk, Kami̇l Işik, Ergi̇n Kari̇ptaş, Reşi̇t Özkanca
Turkish Journal of Biology
Forty-five thermophilic streptomycetes were isolated from different habitats and examined for 163 unit characters with 8 representative thermophilic type strains, 29 reference strains and 16 randomly selected duplicated organisms for many phenotypic properties. The data were evaluated using the simple matching coefficients (S_{SM}), and clustering achieved using the complete algorithm. It was clearly seen that the high cophenetic correlation, low test error values and high similarity level agreed with the defined numerical taxonomic criteria. The test strains were assigned to four aggregate groups consisting seven major (5-8 strains), nine minor (2-4 strains) and ten single membered clusters. It can be …
Effects Of Ph And Aluminium Ion Concentration On Spore Germination And Growth Of Some Soil Fungi, Şükrü Dursun, Lynne Boddy, Juliet C. Frankland
Effects Of Ph And Aluminium Ion Concentration On Spore Germination And Growth Of Some Soil Fungi, Şükrü Dursun, Lynne Boddy, Juliet C. Frankland
Turkish Journal of Biology
Concentrations of three aluminium compounds significantly affected the mycelial extension of Trichoderma viride with low pH values growing on agar media, and the largest effect was recorded with aluminium chloride. Experiments in this work showed that there were significant relationships between Al concentration and pH in the Al inhibition of fungal activity on agar media. Different effects of aluminium concentration on the mycelial extension rate of eight fungal species inoculated on pH gradient plates were recorded. The spore germination of Phoma exigua and Cladosporium herbarum was reduced by increasing aluminium and acidity, and low pH increased the inhibitory effect of …
Effects Of Indoleacetic Acid And Kinetin On Lipid Peroxidation Levels In Various Rat Tissues, İsmai̇l Çeli̇k, Yasi̇n Tülüce, Necati̇ Özok
Effects Of Indoleacetic Acid And Kinetin On Lipid Peroxidation Levels In Various Rat Tissues, İsmai̇l Çeli̇k, Yasi̇n Tülüce, Necati̇ Özok
Turkish Journal of Biology
This study was carried out to investigate whether plant growth regulators (PGRs) could affect lipid peroxidation (Malondialdehyde = MDA level) in erythrocyte, muscle, liver, heart and kidney tissue. Eighteen Sprague-Dawley albino rats were divided into three experimental groups: control and PGR-treated (indoleacetic acid and kinetin). Seventy-five ppm of PGRs, indoleacetic acid (IAA), and kinetin were administered orally to 6 rats ad libitum for 25 days. The hormone treatments caused different effects on the level of malondialdehyde (MDA) to those of the control rats. According to the results, while liver and kidney MDA levels were increased significantly by IAA administration in …
Antimicrobial And Cytotoxic Activities Of Ceratonia Siliqua L. Extracts, Bi̇jen Kivçak, Tuba Mert, H. Tansel Öztürk
Antimicrobial And Cytotoxic Activities Of Ceratonia Siliqua L. Extracts, Bi̇jen Kivçak, Tuba Mert, H. Tansel Öztürk
Turkish Journal of Biology
The antimicrobial and cytotoxic activities of n-hexane, methanol, ethanol, ethyl acetate and water extracts of Ceratonia siliqua L. leaves were evaluated in this study. The antimicrobial activities of the extracts were reported against Escherichia coli ATCC 29998, Escherichia coli ATCC 25922, Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Staphylococcus aureus ATCC6538P, Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Salmonella thyphimurium CCM 5445, Enterobacter cloacae ATCC 13047, Enterococcus faecalis ATCC 29212, Pseudomonas aeroginosa ATCC 27853 as bacteria and Candida albicans ATCC 10239 as yeast-like fungi by the disc diffusion method. The cytotoxic activity of the extracts was evaluated by …
Antimicrobial Peptides: A Potential Therapeutic Alternative For The Treatment Of Fish Diseases, Ali̇ye Sarmaşik
Antimicrobial Peptides: A Potential Therapeutic Alternative For The Treatment Of Fish Diseases, Ali̇ye Sarmaşik
Turkish Journal of Biology
Fish losses from infectious diseases are a significant problem in aquaculture worldwide. However, peptide antibiotics show potent activity against a broad range of pathogens including fish pathogens. Therefore, the ability of antimicrobial peptides to protect fish against infections caused by fish pathogens clearly shows the potential for utilisation of antimicrobial peptides for the treatment of fish diseases. The strategy of overexpressing the antimicrobial peptide genes in transgenic fish may provide a method of decreasing fish disease problems in aquaculture.
Some Properties Of Crude Carboxymethyl Cellulase Of Aspergillus Niger Z10 Wild-Type Strain, Gökhan Coral, Burhan Arikan, M. Ni̇sa Ünaldi, Hati̇ce Güvenmez
Some Properties Of Crude Carboxymethyl Cellulase Of Aspergillus Niger Z10 Wild-Type Strain, Gökhan Coral, Burhan Arikan, M. Ni̇sa Ünaldi, Hati̇ce Güvenmez
Turkish Journal of Biology
A carboxymethyl cellulase enzyme was prepared from a wild type strain of Aspergillus niger Z10. Analyses of the enzyme preparation by SDS-PAGE revealed two protein bands showing cellulolytic activity. The molecular weight of these bands was estimated to be around 83,000 and 50,000. The optimum temperature of the enzyme was observed to be around 40 °C. It was found that the enzyme's activity has a broad pH range between 3 to 9 and 41.2% of the original activity was retained after heat treatment at 90 °C for 15 min.
Epipelic And Epilithic Algae Of The Yedigöller Lakes (Erzurum-Turkey), Bülent Şahi̇n
Epipelic And Epilithic Algae Of The Yedigöller Lakes (Erzurum-Turkey), Bülent Şahi̇n
Turkish Journal of Biology
The epipelic and epilithic algae of the Yedigöller Lakes were studied qualitatively between June and September 2001. As a result 90 taxa (43 belonging to Bacillariophyta, 33 to Chlorophyta, 11 to Cyanophyta and three to Euglenophyta) were determined. Based on the algal community composition, the Yedigöller Lakes can be characterized as oligotrophic.
The Treatment Efficiency Of Plankton In The İvedik Drinking Water Treatment Plant, Ankara, Ni̇lsun Demi̇r, Doğan Atay
The Treatment Efficiency Of Plankton In The İvedik Drinking Water Treatment Plant, Ankara, Ni̇lsun Demi̇r, Doğan Atay
Turkish Journal of Biology
The phyto-zooplankton composition and some physical and chemical properties of drinking water were investigated in the İvedik water treatment plant. The mean values of phytoplankton abundance were 641±92 and 438±55 ind/ml in the Kurtboğazı and Çamlıdere inlets, respectively. More than 50% of the phytoplankton belonging to the classes Bacillariophyceae, Chlorophyceae, Cryptophyceae and Dinophyceae were removed in the clarifiers. The treatment efficiency of phytoplankton ranged from 89 to 100% after the last chlorination. The mean values of zooplankton abundance were 93±23 and 59±18 ind/l in the Kurtboğazı and Çamlıdere inlets, respectively. The treatment efficiency of zooplankton varied between 97% and 100% …
Effects Of Gibberellic Acid Treatment For Pollen Sterility Induction On The Physiological Activity And Endogenous Hormone Levels Of The Seed In Safflower, Hasan Baydar
Turkish Journal of Biology
In this research, our aim was to determine the effects of gibberellic acid, which was applied to safflower plants (Carthamus tinctorius L. cv. Dinçer 5-118) for pollen sterility induction, on some physiological activity and endogenous hormone levels of the seeds. Exogenously applied gibberellic acid (GA_3) strongly influenced the endogenous hormone levels of the seeds by decreasing the levels of GA_3 and zeatin, and increasing the levels of indole-3-acetic acid (IAA) and abscisic acid (ABA). The lowered endogenous GA_3/ABA and zeatin/IAA ratios in the seeds significantly decreased the germination percentage and hypocotyl elongation, respectively. The seeds from GA_3 treated plants had …
Production Of Poly-B-Hydroxybutyrate (Phb) By Some Rhizobium Bacteria, Nazi̇me Mercan, Belma Aslim, Zehra Nur Yüksekdağ, Yavuz Beyatli
Production Of Poly-B-Hydroxybutyrate (Phb) By Some Rhizobium Bacteria, Nazi̇me Mercan, Belma Aslim, Zehra Nur Yüksekdağ, Yavuz Beyatli
Turkish Journal of Biology
In this study, the production of Poly-b-hydroxybutyrate (PHB) was determined in 1 Rhizobium japonicum, 6 Rhizobium cicer, 8 Rhizobium spp. and Bradyrhizobium japonicum USDA 110. The content of according to dry cell weight was determined to be 1.38-40.0%. In our study, Rhizobium spp. 2426, which produced the highest percentage yield of PHB, and Rhizobium spp. 640, which produced the intermediate percentage yield of PHB, were first selected among all the strains, and then the effect of different carbon and nitrogen sources on PHB production in these strains was tested. While the strains produced less PHB in yeast extract mannitol (YEM) …
The Role Of Phospholiphase D In Phospholiphid Transaction During Cotton Seeds Maturation, Muborak Mahmusovna Abdullaeva, Mamura Muhamedovna Mulladganova, Mirzalievna M. Umarova
The Role Of Phospholiphase D In Phospholiphid Transaction During Cotton Seeds Maturation, Muborak Mahmusovna Abdullaeva, Mamura Muhamedovna Mulladganova, Mirzalievna M. Umarova
Turkish Journal of Biology
The role of phospholipase D (EC 3.1.4.4) was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation. To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). Enzyme activity was judged from the formation of hydrolysis products - phosphatidic acid and phosphatidylmethanol. The phospholipid content was defined by using thin-layer chromatography with a follow up analysis of phosphorus quantity in phospholipid fractions. It was shown that phospholipase D displayed two functions during cotton seed maturation: transferase and …
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Cellular Adhesion And Adhesion Molecules, Zerri̇n Seller
Turkish Journal of Biology
In recent years, cell adhesion and cell adhesion molecules have been shown to be important for many normal biological processes, including embryonic cell migration, immune system functions and wound healing. It has also been shown that they contribute to the pathogenesis of a large number of common human disorders, such as rheumatoid arthritis and tumor cell metastasis in cancer. In this review, the basic mechanisms of cellular adhesion and the structural and functional features of adhesion molecules are summarized.
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Expression Of A Fungal Cellulase Gene By \Beta-Glucanase Promoter Of Streptococcus Bovis, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Biology
The promoter region of the §-glucanase gene was identified using a transcriptional fusion between the upstream region of the Streptococcus bovis \beta-glucanase gene and the celA gene. Using the transcriptional and protein localisation signals of the S. bovis \beta-glucanase gene, an in-frame tarnslational fusion between the end of the \beta-glucanase signal sequence and the ATG of the Neocallimastix patriciarum celA gene was constructed. The \beta-glucanase promoter-celA fusion was expressed in both E. coli and S. bovis. The activity of the protein produced was found to be cell-associated in E. coli, it but localised to the supernatant fraction of harvested cells …