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Full-Text Articles in Biology

The Effect Of Hyperosmotic Stress And Nitrogen Starvation On Growth And \Beta-Galactosidase Synthesis In Kluyveromyces Lactis And Kluyveromyces Marxianus, Sezai̇ Türkel, Eli̇f Arik, Si̇nem Güzelvardar Jan 2008

The Effect Of Hyperosmotic Stress And Nitrogen Starvation On Growth And \Beta-Galactosidase Synthesis In Kluyveromyces Lactis And Kluyveromyces Marxianus, Sezai̇ Türkel, Eli̇f Arik, Si̇nem Güzelvardar

Turkish Journal of Biology

Kluyveromyces lactis and Kluyveromyces marxianus are industrial yeasts widely used in the production of the \beta-galactosidase enzyme. Biosynthesis of \beta-galactosidase is controlled by glucose repression. In this study it was demonstrated that the derepression of \beta-galactosidase biosynthesis in these yeast strains is inhibited by high osmotic stress. It was found that the \beta-galactosidase activity of K. lactis and K. marxianus remained approximately at the repressed level when these yeast cells were subjected to NaCl-, KCl-, or sucrose-induced high osmotic stress. Derepression of \beta-galactosidase biosynthesis seems to be more sensitive to high osmotic stress in K. marxianus than in K. lactis. …


Comparative Antioxidant Activity Of Some Edible Plants, Zaha A. El-Agbar, Ashok K. Shakya, Nooman A. Khalaf, Makbula Al-Haroon Jan 2008

Comparative Antioxidant Activity Of Some Edible Plants, Zaha A. El-Agbar, Ashok K. Shakya, Nooman A. Khalaf, Makbula Al-Haroon

Turkish Journal of Biology

Terminalia chebula, Salvia officinalis L., Calamintha incana, Thymus vulgaris L., Matricaria chamomilla, and Nigella sativa are commonly used spices. In the present study the methanolic extracts of these spices were evaluated for their antioxidant activity by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging method. All the methanolic extracts showed significant antioxidant activity. The IC_{50} of the methanolic extracts ranged between 3.8 ± 0.1 and 168.8 ± 2.1 \mug/ml, and that of ascorbic acid was 8.6 ± 0.1 \mug/ml. The study revealed that the consumption of these spices could exert several beneficial effects by virtue of their antioxidant activity.


The Antioxidant Activity Of Some Medicinal Plants, Prateek Kumar Jain, Veerasamy Ravichandran, Simant Sharma, Ram. K. Agrawal Jan 2008

The Antioxidant Activity Of Some Medicinal Plants, Prateek Kumar Jain, Veerasamy Ravichandran, Simant Sharma, Ram. K. Agrawal

Turkish Journal of Biology

The antioxidant properties of the methanol extracts of 10 medicinal plants were evaluated with the DPPH (1,1-diphenyl-2-picryl hydrazyl) test, hydroxyl scavenging test, and lipid peroxidation assay. The methanol extract of Emblica officinalis was the most active according to the DPPH test, with an IC_{50} value of 7.92 ± 0.1 \mug/ml, followed by Asparagus racemosus, with an IC_{50} value of 9.28 ± 0.2 \mug/ml. The minimum free radical scavenging activity was shown by Zingiber officinale, with an IC_{50} value of 44.48 ± 1.8 \mug/ml. The hydroxyl radical scavenging activity of the methanolic extracts of various plants was as follows: Asparagus racemosus …


Antioxidant And Vasorelaxant Activities Of Flavonoids From Amygdalus Lycioides Var. Horrida, Hossein Babaei, Omid Sadeghpour, Lutfun Nahar, Abbas Delazar, Hossein Nazemiyeh, Mohammad Reza Mansouri, Naser Poursaeid, Solmaz Asnaashari, Sedigheh Bamdad Moghadam, Satyajit Dey Sarker Jan 2008

Antioxidant And Vasorelaxant Activities Of Flavonoids From Amygdalus Lycioides Var. Horrida, Hossein Babaei, Omid Sadeghpour, Lutfun Nahar, Abbas Delazar, Hossein Nazemiyeh, Mohammad Reza Mansouri, Naser Poursaeid, Solmaz Asnaashari, Sedigheh Bamdad Moghadam, Satyajit Dey Sarker

Turkish Journal of Biology

Amygdalus lycioides var. horrida (Spach) Browicz (Rosaceae), also known as Prunus lycioides (Spach.) Schneid., is an endemic Iranian species of the genus Amygdalus. In Iranian traditional medicine, the aerial parts and roots of A. lycioides are used in the treatment of diabetes. Six flavonoids, i.e. quercetin 3-O-rhamnoside (1), luteolin 7-O-rhamnoside (2), isorhamnetin 3-O-rutinoside (3), kaempferol 3-O-rhamnoside (4), apigenin (5), and naringenin (6), have been isolated from the aerial parts of this plant. The structures of these compounds were elucidated by UV, MS, and NMR spectroscopic data analyses. While the antioxidant activity of these compounds was assessed by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) …


Specific Individuals Of Rainbow Trout (Oncorhynchus Mykiss) Are Able To Show Time-Place Learning, M. Saeed Heydarnejad, G. J. Purser Jan 2008

Specific Individuals Of Rainbow Trout (Oncorhynchus Mykiss) Are Able To Show Time-Place Learning, M. Saeed Heydarnejad, G. J. Purser

Turkish Journal of Biology

Using demand-feeders, time-place learning association of food was investigated in rainbow trout (Oncorhynchus mykiss) in 2 small raceways (TR1 and TR2). In order for the fish to be visually identified, they were tagged individually and their behaviour and distribution were monitored by cameras. Equipping with a demand-feeder, TR1 was assigned to test 2 times/1 place (2T/1P) while 2 demand-feeders were used in TR2 assigned to test 2 times/2 places (2T/2P). After 26 days of restricted feeding (RF) the fish were deprived of food for 3 days. The results of the present study indicate that, at the group level, rainbow trout …


Chitinase Production By Serratia Marcescens Gg5, Gursharan Singh, Joginder Ram Sharma, Gurinder Singh Hoondal Jan 2008

Chitinase Production By Serratia Marcescens Gg5, Gursharan Singh, Joginder Ram Sharma, Gurinder Singh Hoondal

Turkish Journal of Biology

Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was 0.3 U/ml when the organism was grown in M9 medium supplemented with 0.5% swollen chitin and 0.5% soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase.


The Effects Of Mistletoe (Viscum Album L. Subsp. Album) Extracts On Isolated Intestinal Contractions, Alper Karakaş, Erdi̇nç Seri̇n, Bülent Gündüz, Arzu Uçar Türker Jan 2008

The Effects Of Mistletoe (Viscum Album L. Subsp. Album) Extracts On Isolated Intestinal Contractions, Alper Karakaş, Erdi̇nç Seri̇n, Bülent Gündüz, Arzu Uçar Türker

Turkish Journal of Biology

Throughout history folk medicine has served as an alternative or complimentary method of treating disorders like intestinal colic. Mistletoe is a common name for many species of semi-parasitic plants that grow on trees throughout the world, and has been used widely to treat intestinal colic. We conducted a study to evaluate the effect of freeze-dried (group 1) and heat-treated (group 2) extracts of Viscum album subsp. album leaves on contractions induced by acetylcholine in isolated hamster intestinal segments (duodenum, jejunum, and ileum). Freeze-dried extracts decreased intestinal contractions in all the intestinal segments (P < 0.05). The strongest relaxant effect was observed in the jejunum and the weakest was seen in the duodenum. Higher doses of heat-treated extracts were required to affect segmental contractions than that of freeze-dried extracts (P < 0.05); heat-treated extracts had the greatest effect on reducing contractions in the jejunum and the least effect in the duodenum. Mistletoe extracts affected the level of contractions in a dose- and extraction preparation-dependent manner. The results of this study show that mistletoe extracts could be effective against intestinal motility problems and that they have potential use as a therapeutic remedy for intestinal colic.


Optimization Of Process Parameters For Alkaline Protease Production By Bacillus Licheniformis N-2 And Kinetics Studies In Batch Fermentation, Muhammad Nadeem, Javed Iqbal Qazi, Qurat-Ul-Ain Syed, Shahjahan Baig Jan 2008

Optimization Of Process Parameters For Alkaline Protease Production By Bacillus Licheniformis N-2 And Kinetics Studies In Batch Fermentation, Muhammad Nadeem, Javed Iqbal Qazi, Qurat-Ul-Ain Syed, Shahjahan Baig

Turkish Journal of Biology

Production of alkaline protease from B. licheniformis N-2 was studied in shake flask experiments in growth medium comprised of (g/l): glucose, 10; soybean meal, 10; K_2HPO_4, 5; MgSO_4.7H_2O, 0.5; NaCl, 0.5; CaCl_2.2H_2O, 0.5. Different process parameters, such as fermentation period, initial pH, incubation temperature, agitation speed, and size and age of inoculum, were optimized for the maximum yield of the alkaline protease. Maximum enzyme production (991.04 PU/ml) was obtained after 24 h of incubation at 37 °C in growth medium of pH 10. The optimum inoculum size and age were determined as 2.0% (v/v) and 24 h, respectively. Kinetics of …


The Interaction Effects Of Flooding And Kinetin On Growth Criteria, Chlorophyll Content, And 5-Aminolevulinic Acid Dehydratase Activity In Corn Seedlings, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare Jan 2008

The Interaction Effects Of Flooding And Kinetin On Growth Criteria, Chlorophyll Content, And 5-Aminolevulinic Acid Dehydratase Activity In Corn Seedlings, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare

Turkish Journal of Biology

The interaction effects of kinetin and flooding on growth indexes, chlorophyll content, and the activity of 5-aminolevulinic acid dehydratase (ALA-D; EC 4.2.1.24) in seedlings of corn (Zea mays L. cv. 704) were investigated. Samples were taken 48, 96, 144, and 192 h after the start of treatment. Growth criteria (root length, shoot height, and dry weight) were inhibited more in flooded plants than in control plants. The pattern of growth changes was similar to chlorophyll a and b, as well as ALA-D activity. The exogenous application of kinetin (100 ppm) nullified the resulting decrease in growth and chlorophyll, but only …


In Vivo Anti-Ulcerogenic Activity Of Equisetum Telmateia Ehrh. Extracts Used In Turkish Folk Medicine, İlhan Gürbüz, Erdem Yeşi̇lada Jan 2008

In Vivo Anti-Ulcerogenic Activity Of Equisetum Telmateia Ehrh. Extracts Used In Turkish Folk Medicine, İlhan Gürbüz, Erdem Yeşi̇lada

Turkish Journal of Biology

The aerial part of Equisetum telmateia Ehrh. is used against various conditions including stomachache and peptic ulcer in Turkish folk medicine. In order to prove the claimed anti-ulcerogenic potential of the plant, aqueous and methanol extracts were prepared from aerial parts of E. telmateia and investigated for their anti-ulcerogenic effect on ethanol-induced gastric ulcer model in rats. Pharmacological experiments clearly demonstrated that the oral administration of both methanol and aqueous extracts of Equisetum telmateia Ehrh. plant showed significant stomach protection (77.9% and 100% ulcer inhibition, respectively) against the applied model of ulcerogenesis (P < 0.001). Since both extracts were found significantly active, 80% ethanol was preferred for the extraction of the plant material and this crude methanolic extract was further fractionated by successive solvent extractions with chloroform and H_2O saturated n-BuOH to obtain the chloroform and n-BuOH extracts as well as the remaining aqueous extract. The 80% ethanol extract and the solvent extracts were then submitted to pharmacological assay using the same in vivo experimental ulcer model. All the extracts statistically showed potent anti-ulcerogenic effect on this model (P < 0.001). However, the remaining aqueous extract was found prominent (98.2% ulcer inhibition). Consequently, folkloric utilization of E. telmateia on peptic ulcer has been confirmed in the present study by using ethanol-induced experimental ulcer model in rats.


Determination Of Antimicrobial And Antioxidant Activities Of Turkish Endemic Salvia Halophila Hedge, Sevi̇l Albayrak, Ahmet Aksoy, Ergi̇n Hamzaoğlu Jan 2008

Determination Of Antimicrobial And Antioxidant Activities Of Turkish Endemic Salvia Halophila Hedge, Sevi̇l Albayrak, Ahmet Aksoy, Ergi̇n Hamzaoğlu

Turkish Journal of Biology

In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC_{50} of 67.73 \mug/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallic acid equivalent …


Investigation Of The Antimutagenic Potentials Of The Methanol Extract Of Origanum Vulgare L. Subsp. Vulgare In The Eastern Anatolia Region Of Turkey, Tüli̇n Özbek, Medi̇ne Güllüce, Fi̇kretti̇n Şahi̇n, Hakan Özkan, Sevgi̇ Sevsay, Özlem Bariş Jan 2008

Investigation Of The Antimutagenic Potentials Of The Methanol Extract Of Origanum Vulgare L. Subsp. Vulgare In The Eastern Anatolia Region Of Turkey, Tüli̇n Özbek, Medi̇ne Güllüce, Fi̇kretti̇n Şahi̇n, Hakan Özkan, Sevgi̇ Sevsay, Özlem Bariş

Turkish Journal of Biology

The aim of this study was to evaluate the antimutagenic activity of the methanol extract of Origanum vulgare L. subsp. vulgare. Antimutagenic activity was estimated by employing the plate incorporation AMES/Salmonella histidine reversion assay. The base pair substitution tester strain Salmonella typhimurium TA1535 and the frame shift mutagen strain S. typhimurium TA1538 were used against direct acting mutagens - sodium azide (NaN_3), 4-nitro-1-quinoline oxide (4NQO) and the S9-dependent mutagen 2-aminofluorene (2AF). The results suggested that in the absence of S9 metabolic activation, all 3 doses (5, 0.5, and 0.05 \mug/plate) of the plant extract tested caused statistically significant (P < 0.05) antimutagenic activity on TA1535 strain, but not on TA1538 strain. However, in the presence of S9 microsomal fraction, the plant extract exerted moderate antimutagenic activity against the 2AF mutagen and reduced mutant colonies in TA1535 and TA1538 strains. As a result, the methanol extract of Origanum vulgare subsp. vulgare showed antimutagenic effects at 5, 0.5, and 0.05 \mug/plate concentrations. These effects may be explained with the antioxidant activity mechanism, changes in membrane lipids, and permeability of ion channels.


Analysis Of Ki-Ras Exon 2 Gene Mutations In 3-Methylcholanthrene And Butylated Hydroxytoluene-Induced Rat Lung Tissues, Fi̇kri̇ye Polat, Öztürk Özdemi̇r, Şahende Elagöz Jan 2008

Analysis Of Ki-Ras Exon 2 Gene Mutations In 3-Methylcholanthrene And Butylated Hydroxytoluene-Induced Rat Lung Tissues, Fi̇kri̇ye Polat, Öztürk Özdemi̇r, Şahende Elagöz

Turkish Journal of Biology

3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung …


The Effects Of Micronuclei With Whole Chromosome On Biological Dose Estimation, Funda Si̇bel Pala, Fadi̇me Alkaya, Kiymet Tabakçioğlu, Füsun Tokatli, Cem Uzal, Şule Parlar, Çeti̇n Algüneş Jan 2008

The Effects Of Micronuclei With Whole Chromosome On Biological Dose Estimation, Funda Si̇bel Pala, Fadi̇me Alkaya, Kiymet Tabakçioğlu, Füsun Tokatli, Cem Uzal, Şule Parlar, Çeti̇n Algüneş

Turkish Journal of Biology

The total micronucleus (MN) assay has been used for purposes of biological dosimetry for many years. The variable spontaneous incidence of micronuclei in peripheral blood lymphocytes affects the sensitivity of biological dose estimations at low doses. It has been suggested that this problem could be solved by using the micronuclei-centromere assay. In this study, Co-60 gamma ray dose response curves for micronuclei (MN) and micronuclei without centromeres (MNC-) in the range of 0-5.0 Gy were established using a pancentromeric FISH probe on cultured binucleate lymphocytes from 2 donors. There were no significant inter-donor differences in the dose responses for either …


Phytoplankton Species Composition And Seasonal Variation At Wadi Al-Arab Dam Lake, Jordan, Ismail M. Saadoun, Erwah Bataineh, Adil Alhandal Jan 2008

Phytoplankton Species Composition And Seasonal Variation At Wadi Al-Arab Dam Lake, Jordan, Ismail M. Saadoun, Erwah Bataineh, Adil Alhandal

Turkish Journal of Biology

The composition of phytoplankton species thriving in Wadi Al-Arab Dam Reservoir, Jordan and their seasonal variation in relation to water ecology were studied between February 2001 and February 2002. A total of 75 species of phytoplankton were identified: Bacillariophyceae (diatoms) contributed with the highest number of taxa (39) followed by Chlorophyceae (23), Cyanophyceae (9), and Dinophyceae (4). Diatoms constituted 52% of the total phytoplankton flora of the reservoir and the most common species were Synedra ulna, Cymbella affinis, and Cyclotella meneghiniana with pennate and centric diatoms being the dominant species in May and October-November, respectively. Although Chlorophytes occurred most of …


Plant Growth Promoting Properties Of Rhizobacteria Isolated From Wheat And Pea Grown In Loamy Sand Soil, Dilfuza Egamberdieva Jan 2008

Plant Growth Promoting Properties Of Rhizobacteria Isolated From Wheat And Pea Grown In Loamy Sand Soil, Dilfuza Egamberdieva

Turkish Journal of Biology

Microbes are important catalysts to regulate functional properties of terrestrial ecosystems. In this study, rhizosphere and phyllosphere bacteria were isolated from wheat and peas and examined for their plant growth promoting properties. The effects of bacterial inoculants on the growth of peas and wheat were studied in a series of pot experiments using loamy sand soil. The results showed that the colonisation of bacteria was higher in the rhizosphere as compared to the phyllosphere of both plants. Bacterial strains were identified as Pseudomonas, Bacillus, Kocuria, and Microbacterium, and Cellulomonas species. The response of wheat and peas when inoculated with bacteria …


A Comparative Study On Colchicine Application Methods In Obtaining Doubled Haploids Of Tobacco (Nicotiana Tabacum L.), Betül Bürün, Ülkü Emi̇roğlu Jan 2008

A Comparative Study On Colchicine Application Methods In Obtaining Doubled Haploids Of Tobacco (Nicotiana Tabacum L.), Betül Bürün, Ülkü Emi̇roğlu

Turkish Journal of Biology

In order to obtain androgenic doubled haploids of tobacco (Nicotiana tabacum cv. Karabağlar 6265) colchicine was applied at 3 different stages of anther culture. Before culture, anthers were treated with 0.4% aqueous solution of colchicine for 0, 2, 4, 6, 8, 10, and 12 h. Culture response of anthers decreased as the treatment duration increased (except 12 h) and the highest diploidization of 29.7% was obtained with 6 h. During culture, when macroscopic embryoids appeared in dehisced anthers, they were wrapt up in sterile cotton saturated with 0.2% colchicine for 3 days and transferred to fresh medium. This application resulted …


Life In The Absence Of Oxygen: Alternative Electron Acceptors For Anaerobic Microorganisms In A Petroleum Environment, Meli̇ke Balk Jan 2007

Life In The Absence Of Oxygen: Alternative Electron Acceptors For Anaerobic Microorganisms In A Petroleum Environment, Meli̇ke Balk

Turkish Journal of Biology

Anaerobic microorganisms derive energy by transferring electrons from an external source or donor to an external electron sink or terminal acceptor and often have the capacity to reduce 2 or more terminal electron acceptors. The well-known type of microbial respiration, in which oxygen serves as an electron acceptor for the oxidation of organic carbon and/or hydrogen, has been studied elsewhere in detail. Anaerobic microorganisms are widely distributed in oil-producing vents, hydrothermal vents, volcanic hot springs, non-volcanic geothermally heated subsurface aquifers, and soil. In this study, anaerobic, thermophilic, and fermenting microorganisms in a petroleum sample from the Adıyaman region of Turkey …


Identification And Characterization Of The Rat Dvl2 Gene Using Bioinformatic Tools, Lokman Varişli, Osman Çen Jan 2007

Identification And Characterization Of The Rat Dvl2 Gene Using Bioinformatic Tools, Lokman Varişli, Osman Çen

Turkish Journal of Biology

We identified and characterized the rat DVL2 gene using bioinformatics. In addition to the structure and chromosomal localization of the rat DVL2 gene, the transcribed and translated protein product of the gene was analyzed in silico. Results showed that the rat DVL2 gene consists of 15 exons and is located on the rat genomic contig WGA1854.3 on chromosome 10. Database searches using the rat DVL2 amino acid sequence as a query showed a number of homologous protein sequences in different species, including M. musculus, P. troglodytes, C. familiaris, H. sapiens, B. taurus, D. rerio, X. laevis, and T. nigroviridis. DAX, …


In Vitro Antibacterial Studies Of Ciprofloxacin-Imines And Their Complexes With Cu(Ii),Ni(Ii),Co(Ii), And Zn(Ii), Muhammad Imran, Javed Iqbal, Shahid Iqbal, Nazia Ijaz Jan 2007

In Vitro Antibacterial Studies Of Ciprofloxacin-Imines And Their Complexes With Cu(Ii),Ni(Ii),Co(Ii), And Zn(Ii), Muhammad Imran, Javed Iqbal, Shahid Iqbal, Nazia Ijaz

Turkish Journal of Biology

Some new transition metal complexes of ciprofloxacin-imines derived from ciprofloxacin and p-substituted anilines were synthesized and characterized on the basis of physical properties, conductance measurements, elemental analysis, UV/Vis., infrared and nuclear magnetic resonance spectroscopy. These ligands as well as their metal complexes were also evaluated for their antibacterial activity against several bacterial strains, such as Staphylococcus aureus, Bacillus subtilus, Salmonella typhae, and E. coli. It was found that metal complexes are more antibacterial as compared to uncomplexed ligands. The present study was carried out in search of a target antibiotic instead of a broad spectrum one.


Molecular Characterization Of Borrelia Burgdorferi Linear Plasmids By Dna Hybridization, Pcr, Two-Dimensional Gel Electrophoresis, And Electron Microscopy, Ali̇ Karami, Seyed Javad Hosseyni, Yaser Kiarudi Jan 2007

Molecular Characterization Of Borrelia Burgdorferi Linear Plasmids By Dna Hybridization, Pcr, Two-Dimensional Gel Electrophoresis, And Electron Microscopy, Ali̇ Karami, Seyed Javad Hosseyni, Yaser Kiarudi

Turkish Journal of Biology

Borrelia burgdorferi sensu stricto is the predominant cause of Lyme disease. B. burgdorferi strains carry several linear and circular plasmids and this may be also used as a distinguishing property of the bacteria. The presence of a mixed population of linear and circular plasmids makes separation and characterization of each kind very difficult. In this study, 7 clinical strains of skin and CSF isolates were determined by clonal selection and plasmid profile of B. burgdorferi by electron microscopy and 2-dimensional agarose gel electrophoresis. The results demonstrate that the skin isolate (DK1) has 2 different phenotypes A and B, but the …


Maternal Transfer Of Photoperiodic Information Regulates The Postnatal Reproductive System Development Of The Mongolian Gerbil (Meriones Unguiculatus), Alper Karakaş, Bülent Gündüz Jan 2007

Maternal Transfer Of Photoperiodic Information Regulates The Postnatal Reproductive System Development Of The Mongolian Gerbil (Meriones Unguiculatus), Alper Karakaş, Bülent Gündüz

Turkish Journal of Biology

We investigated whether photoperiodic information is transferred from mother to young and whether the pineal gland and its hormone, melatonin, are involved in this transfer in Mongolian gerbils. Adult female gerbils were paired with males and housed in photoperiods of 12L:12D or 8L:16D. Three groups of pregnant gerbils in each photoperiod were prepared: one group was the sham-operated control, one group consisted of pinealectomized gerbils, and one group consisted of pinealectomized gerbils that were treated with melatonin. Pinealectomy was performed before pairing the animals and melatonin injections were applied to the pinealectomized group from day 8 of gestation until parturition. …


Protective Potential Of Glimepiride And Nerium Oleander Extract On Lipid Profile, Body Growth Rate, And Renal Function In Streptozotocin-Induced Diabetic Rats, Maged M. Yassin, Saleh N. Mwafy Jan 2007

Protective Potential Of Glimepiride And Nerium Oleander Extract On Lipid Profile, Body Growth Rate, And Renal Function In Streptozotocin-Induced Diabetic Rats, Maged M. Yassin, Saleh N. Mwafy

Turkish Journal of Biology

This study aimed to assess the protective potential of glimepiride and Nerium oleander extract on lipid profile, body growth rate, and renal function in streptozotocin-induced diabetic rats. The animals were divided into control and experimental groups. The experimental group was rendered diabetic by intraperitoneal injection of a single dose of 50 mg/kg body weight streptozotocin. Rats with glucose levels >200 mg/dl were subdivided into 3 sub-groups. Rats in the first sub-group remained without treatment and were considered diabetic. Those in the second and third subgroups were orally administered 0.1 mg/kg body weight daily glimepiride and 250 mg/kg body weight daily …


Photosynthetic And Yield Responses Of Nigella Sativa L. To Pre-Sowing Seed Treatment With Ga3, Shoukat H. Shah Jan 2007

Photosynthetic And Yield Responses Of Nigella Sativa L. To Pre-Sowing Seed Treatment With Ga3, Shoukat H. Shah

Turkish Journal of Biology

Surface sterilized seeds of black cumin (Nigella sativa L.) were soaked in 10^{-6}, 10^{-5}, or 10^{-4} M aqueous solution of gibberellic acid (GA_3) for 5, 10, or 15 h and sown in pots with sandy loam soil. The plants were then sampled at 50, 70, and 90 days after sowing, for the analysis of shoot length, leaf area, dry mass, carbonic anhydrase activity (E.C. 4.2.1.1), leaf chlorophyll content, stomatal conductance, and net photosynthetic rate. Seed yield was recorded at harvest (130 DAS). All parameters were found to be significantly enhanced by the hormone (GA_3) treatment, with maximum stimulation being noted …


What Is The Relationship Within The Family Lamnidae?, Mi̇ne Dosay Akbulut Jan 2007

What Is The Relationship Within The Family Lamnidae?, Mi̇ne Dosay Akbulut

Turkish Journal of Biology

Uncertainties remain about the relationship within the family Lamnidae, which includes the white shark, 2 makos, the porbeagle, and salmon shark. Several alternative hypotheses have been suggested related to the Lamnidae phylogeny. The aim of this study was to find an accurate answer to the family Lamnidae interrelationship. To that extend, the mitochondrial cytochrome b gene was studied in all the Lamnidae species and 2 outgroups from the families Alopiidae and Odontaspididae. The monophyly of 2 makos were obtained from both DNA and amino acid (AA) parsimony analysis. Based on our analysis, first the white shark joins this group, which …


Defoliation Effects On Sunflower (Helianthus Annuus L.) Seed Yield And Oil Quality, Sabri̇ Erbaş, Hasan Baydar Jan 2007

Defoliation Effects On Sunflower (Helianthus Annuus L.) Seed Yield And Oil Quality, Sabri̇ Erbaş, Hasan Baydar

Turkish Journal of Biology

The objective of the present study was to determine the influence of artificial defoliation on sunflower seed yield, oil content, and fatty acid composition. The research was carried out at Süleyman Demirel University, Isparta, Turkey, during 2002 and 2003. Six defoliation levels [0 (control), 5, 10, 15, 20 and 25 leaves per plant] were applied at the preflowering stage from bottom to top leaves. The results demonstrate a close relationship between yield reduction and the defoliation levels. The higher the defoliation level, the higher the yield reduction. Complete and partial leaf removal treatments reduced oil percentage of seed when compared …


Modified Path To High Lint Yield In Upland Cotton (Gossypium Hirsutum L.) Under Two Temperature Regimes, Saeed Rauf, Tariq Manzoor Khan, Abdul Naveed, Hassan Munir Jan 2007

Modified Path To High Lint Yield In Upland Cotton (Gossypium Hirsutum L.) Under Two Temperature Regimes, Saeed Rauf, Tariq Manzoor Khan, Abdul Naveed, Hassan Munir

Turkish Journal of Biology

In this study 23 genotypes, including 8 cultivars and 15 advanced lines were consecutively grown under 2 temperature regimes in 2004 and 2005 at the Post Graduate Agriculture Research Station, University of Agriculture, Faisalabad, Pakistan. Lint yield and its component traits, i.e. lint weight per boll, lint weight per seed, fiber length, fibers per seed, fibers per unit surface area, lint weight per unit surface area, and fiber weight per unit surface area, were measured and analyzed for heritability, genetic advance, and correlations. Phenotypic correlations were also partitioned into path coefficients, keeping lint yield as the resultant variable and other …


Changes In Growth, Biochemical Constituents, And Antioxidant Potentials In Radish (Raphanus Sativus L.) Under Cobalt Stress, Kaliyamoorthy Jayakumar, Cheruth Abdul Jaleel, Packirisamy Vijayarengan Jan 2007

Changes In Growth, Biochemical Constituents, And Antioxidant Potentials In Radish (Raphanus Sativus L.) Under Cobalt Stress, Kaliyamoorthy Jayakumar, Cheruth Abdul Jaleel, Packirisamy Vijayarengan

Turkish Journal of Biology

The effects of cobalt (Co) stress on seedling vigor, photosynthetic pigment content, biochemical constituents, mineral status, and the activity of antioxidant enzymes in Raphanus sativus L. were studied. The plants were raised in earthen pots containing soils amended with different concentrations of Co (50, 100, 150, 200, and 250 mg kg^{-1}). Growth parameters (root and shoot length, and total leaf area), photosynthetic pigments content (chlorophyll a, chlorophyll b, and total chlorophyll), biochemical constituents (total sugar, amino acid, and protein content), mineral content (macro- and micronutrients), and the activity of antioxidant enzymes (catalase (CAT), peroxidase (POX), and polyphenol oxidase (PPO)) were …


Cadmium-Induced Changes In The Growth And Carbonic Anhydrase Activity Of Chickpea, Syed Aiman Hasan, Barket Ali, Shamsul Hayat, Aqil Ahmad Jan 2007

Cadmium-Induced Changes In The Growth And Carbonic Anhydrase Activity Of Chickpea, Syed Aiman Hasan, Barket Ali, Shamsul Hayat, Aqil Ahmad

Turkish Journal of Biology

The seedlings of Cicer arietinum, grown in sand culture, were supplied with 0, 50, 100 or 150 µM of cadmium in the form of cadmium chloride at 5, 15 or 25 day stage and assessed for specific characteristics, 10 days after each treatment. It was a general observation in all the samplings that length, fresh and dry mass of both root and shoot and activity of carbonic anhydrase in leaf decreased, whereas proline content both in leaf and root increased. The intensity of the damage was proportionate with the concentration of the metal but was prominent at the earliest stage …


Purification And Partial Characterization Of Intracellular Fructosyltransferase From A Novel Strain Of Aureobasidium Pullulans, Agbaje Lateef, Julius Kola Oloke, Siddalingaiya G. Prapulla Jan 2007

Purification And Partial Characterization Of Intracellular Fructosyltransferase From A Novel Strain Of Aureobasidium Pullulans, Agbaje Lateef, Julius Kola Oloke, Siddalingaiya G. Prapulla

Turkish Journal of Biology

The intracellular fructosyltransferase (FTase) of a novel strain of Aureobasidium pullulans (CFR 77) capable of producing 59% of fructooligosaccharides (FOS) within 9 h of reaction time was obtained by wet-milling, and then purified and characterized. The purified FTase revealed 2 bands of 147 and 170 KD; its activity was optimum at an approximate pH of 5.0 and temperature of 55 °C. The specific activity of the final purified material was 42, representing a purification factor of 79.44 and yield of 43%. The enzyme is very stable, retaining more than 80% of its original activity at the optimum reaction conditions after …