Open Access. Powered by Scholars. Published by Universities.®

Biology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Smith College

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 331 - 340 of 340

Full-Text Articles in Biology

Cloning And Comparison Of Repeated Dna Sequences From The Human Filarial Parasite Brugia Malayi And The Animal Parasite Brugia Pahangi, Larry A. Mcreynolds, Susan M. Desimone, Steven A. Williams Feb 1986

Cloning And Comparison Of Repeated Dna Sequences From The Human Filarial Parasite Brugia Malayi And The Animal Parasite Brugia Pahangi, Larry A. Mcreynolds, Susan M. Desimone, Steven A. Williams

Biological Sciences: Faculty Publications

No abstract provided.

Go to article

Metatherian Reproduction: Transitional Or Transcending?, Virginia Hayssen, R. C. Lacy, P. J. Parker Jan 1985

Metatherian Reproduction: Transitional Or Transcending?, Virginia Hayssen, R. C. Lacy, P. J. Parker

Biological Sciences: Faculty Publications

The major difference between metatherian and eutherian reproductive patterns is the relative apportionment of maternal resources to gestation versus lactation, not the total temporal and energetic investment in each reproductive attempt. Gestation accounts for an average of 12% of the time from conception to weaning in marsupials; in placental mammals it accounts for 56% of this period of development. Parental investment at birth is less in marsupials (litter weight averages 0.09% of female body mass) than in eutherians (average 15.5%), but total maternal effort is comparable in the two groups, since the litter weight at weaning is similar for metatherians …

Go to article

Cloning The Gene For The Malolactic Fermentation Of Wine From Lactobacillus Delbrueckii In Escherichia Coli And Yeasts., Steven A. Williams, Robert A. Hodges, Terry L. Strike, Richard Snow, Ralph E. Kunkee Feb 1984

Cloning The Gene For The Malolactic Fermentation Of Wine From Lactobacillus Delbrueckii In Escherichia Coli And Yeasts., Steven A. Williams, Robert A. Hodges, Terry L. Strike, Richard Snow, Ralph E. Kunkee

Biological Sciences: Faculty Publications

No abstract provided.

Go to article

Macrophages Contain At Least Two Myosins, John A. Trotter, Stylianos P. Scordilis, Sarkis S. Margossian May 1983

Macrophages Contain At Least Two Myosins, John A. Trotter, Stylianos P. Scordilis, Sarkis S. Margossian

Biological Sciences: Faculty Publications

A number of motile functions of macrophages are thought to be mediated by myosin. We have observed that myosin from rabbit alveolar macrophages is heterogeneous with respect to its 20 kDa light chain: two species of 20 kDa light chain are identified by one-dimensional and two-dimensional polyacrylamide gel electrophoresis, in a ratio of 2:1. Native myosin, analyzed on non-denaturing gels, is also composed of two species, in a ratio of 2:1. These results indicate that macrophages contain at least two different myosins, which might have different physiological functions.

Go to article

Changes In Myosin And Myosin Light Chain Kinase During Myogenesis, Stylianos P. Scordilis, J. Maurice Miles, Robert S. Adelstein, B. William Uhlendorf, Sigfrido Scarpa, Giulio L. Cantoni Jan 1981

Changes In Myosin And Myosin Light Chain Kinase During Myogenesis, Stylianos P. Scordilis, J. Maurice Miles, Robert S. Adelstein, B. William Uhlendorf, Sigfrido Scarpa, Giulio L. Cantoni

Biological Sciences: Faculty Publications

Myosins and myosin light chain kinases have been isolated from a cloned line of myoblasts (L5/A10) as this cell line undergoes differentiation toward adult muscle. At least three myosin isozymes were obtained during this developmental process. Initially a nonmuscle type of myosin was found in the myoblasts. The molecular weights of the myoblast light chains were 20 000 and 15 000. Myosin isolated from early myotubes had light chains with molecular weights of 20 000 and 19 500. Myosin isolated from myotubes which contained sarcomeres had light chains with molecular weights of 23 000, 18 500, and 16000. This last …

Go to article

A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein Dec 1978

A Comparative Study Of The Myosin Light Chain Kinases From Myoblast And Muscle Sources. Studies On The Kinases From Proliferative Rat Myoblasts In Culture, Rat Thigh Muscle, And Rabbit Skeletal Muscle, S. P. Scordilis, R. S. Adelstein

Biological Sciences: Faculty Publications

Myosin light chain kinases have been isolated from rat thigh and rabbit skeletal muscle and cultured rat myoblasts. From these preparations, two types of kinases can be distinguished: calcium-dependent and calcium-independent. Both types of kinases can phosphorylate isolated P-light chains of myosin from several sources (skeletal muscle, cardiac muscle, and platelet). Data are shown which support the phosphorylation of the same site on the non-muscle P-light chains by both types of kinases. The rates of these reactions are, however, different for the two types of kinases. Kinetic analysis of the myoblast kinase show differing affinities for various P-light chains (non-muscle …

Go to article

Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi Jul 1978

Membrane Potentials And Resistances Of Giant Mitochondria: Metabolic Dependence And The Effects Of Valinomycin, Bruce L. Maloff, Stylianos P. Scordilis, Christopher Reynolds, Henry Tedeschi

Biological Sciences: Faculty Publications

The membrane potentials and resistances of giant mitochondria from mice fed cuprizone have been studied. They were found to correspond approx. 10-20 mV, positive inside, and 2 M omega, respectively. These properties were found to be independent of the metabolic state. The microelectrodes were in the inner mitochondrial space since (a) the potentials in the presence of valinomycin depended on the K+ concentration of the medium and magnitude of the K+ diffusion potentials was consistent with the presence of a high internal concentration of K+, (b) almost identical results were obtained with mitochondria from which the external membrane had been …

Go to article

Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi Jan 1978

Assays Of The Metabolic Viability Of Single Giant Mitochondria. Experiments With Intact And Impaled Mitochondria, B. L. Maloff, S. P. Scordilis, H. Tedeschi

Biological Sciences: Faculty Publications

Single giant mitochondria isolated from mice fed cuprizone were assayed for their metabolic viability. Two tests were devised. One test optically detected the accumulation of calcium phosphate within the mitochondria under massive loading conditions (including the presence of succinate and ATP). The accumulation corresponds to a test of energy coupling from either electron transport or the hydrolysis of ATP since it is blocked by either antimycin A or oligomycin. The other assay tested for the production of ATP from ADP and Pi, using myofibrils. Myofibrils prepared from glycerinated rabbit psoas muscle contract only in the presence of ATP and not …

Go to article

Characterization Of The Myosin-Phosphorylating System In Normal Murine Astrocytes And Derivative Sv40 Wild-Type And A-Mutant Transformants, S. P. Scordilis, J. L. Anderson, R. Pollack, R. S. Adelstein Jan 1977

Characterization Of The Myosin-Phosphorylating System In Normal Murine Astrocytes And Derivative Sv40 Wild-Type And A-Mutant Transformants, S. P. Scordilis, J. L. Anderson, R. Pollack, R. S. Adelstein

Biological Sciences: Faculty Publications

Myosin and myosin light-chain kinase have been isolated and characterized from small quantities of normal and SV40-transformed, murine astrocytic neuroglial cells in culture and from intact normal mouse brain. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the astrocyte myosins revealed a heavy chain of 200,000 daltons and two light chains of 20,000 and 15,000 daltons. These myosins are similar to other cytoplasmic myosins. The astrocyte 20,000-dalton light chain can be phosphorylated by an endogenous myosin light- chain kinase which has properties similar to those of the myosin light-chain kinase found in human platelets. No differences were detected in either the …

Go to article

Donnan Potential Of Rabbit Skeletal Muscle Myofibrils I: Electrofluorochromometric Detection Of Potential, S. P. Scordilis, H. Tedeschi, C. Edwards Jan 1975

Donnan Potential Of Rabbit Skeletal Muscle Myofibrils I: Electrofluorochromometric Detection Of Potential, S. P. Scordilis, H. Tedeschi, C. Edwards

Biological Sciences: Faculty Publications

The fluorescence of the dye CC 6 [(3 hexyl 2 (3 hexyl 2 benzoxazolinylidene) 1 propenyl) benzoxazolium iodide] was shown to indicate Donnan potentials in rabbits skeletal muscle myofibrils. These results are in aggreement with previously published work in which the potentials were measured with microelectrodes on glycerol extracted muscle fibers. The magnitude of the Donnan potential of the myofibrils was shown to be dependent on the state (rigor or relaxed) of the system.

Go to article

First Prev