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Articles 271 - 300 of 414
Full-Text Articles in Biology
Direct Profiling Of Environmental Microbial Populations By Thermal Dissociation Analysis Of Native Rrnas Hybridized To Oligonucleotide Microarrays, Said El Fantroussi, Hidetoshi Urakawa, Anne E. Bernhard, John J. Kelly, Peter A. Noble, H Smidt, G M. Yershov, David A. Stahl
Direct Profiling Of Environmental Microbial Populations By Thermal Dissociation Analysis Of Native Rrnas Hybridized To Oligonucleotide Microarrays, Said El Fantroussi, Hidetoshi Urakawa, Anne E. Bernhard, John J. Kelly, Peter A. Noble, H Smidt, G M. Yershov, David A. Stahl
Biology: Faculty Publications and Other Works
Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.
Parallel Characterization Of Anaerobic Toluene-And Ethylbenzene-Degrading Microbial Consortia By Pcr-Denaturing Gradient Gel Electrophoresis, Rna-Dna Membrane Hybridization, And Dna Microarray Technology, Yoshikazu Koizumi, John J. Kelly, Tatsunori Nakagawa, Hidetoshi Urakawa, Said El Fantroussi, Saleh Al-Muzaini, Manubu Fukui, Yoshikuni Urushigawa, David A. Stahl
Parallel Characterization Of Anaerobic Toluene-And Ethylbenzene-Degrading Microbial Consortia By Pcr-Denaturing Gradient Gel Electrophoresis, Rna-Dna Membrane Hybridization, And Dna Microarray Technology, Yoshikazu Koizumi, John J. Kelly, Tatsunori Nakagawa, Hidetoshi Urakawa, Said El Fantroussi, Saleh Al-Muzaini, Manubu Fukui, Yoshikuni Urushigawa, David A. Stahl
Biology: Faculty Publications and Other Works
A mesophilic toluene-degrading consortium (TDC) and an ethylbenzene-degrading consortium (EDC) were established under sulfate-reducing conditions. These consortia were first characterized by denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR-amplified 16S rRNA gene fragments, followed by sequencing. The sequences of the major bands (T-1 and E-2) belonging to TDC and EDC, respectively, were affiliated with the family Desulfobacteriaceae. Another major band from EDC (E-1) was related to an uncultured non-sulfate-reducing soil bacterium. Oligonucleotide probes specific for the 16S rRNAs of target organisms corresponding to T-1, E-1, and E-2 were designed, and hybridization conditions were optimized for two analytical formats, membrane and …
Single-Base-Pair Discrimination Of Terminal Mismatches By Using Oligonucleotide Microarrays And Neural Network Analyses, Hidetoshi Urakawa, Peter A. Noble, Said El Fantroussi, John J. Kelly, David A. Stahl
Single-Base-Pair Discrimination Of Terminal Mismatches By Using Oligonucleotide Microarrays And Neural Network Analyses, Hidetoshi Urakawa, Peter A. Noble, Said El Fantroussi, John J. Kelly, David A. Stahl
Biology: Faculty Publications and Other Works
The effects of single-base-pair near-terminal and terminal mismatches on the dissociation temperature (T(d)) and signal intensity of short DNA duplexes were determined by using oligonucleotide microarrays and neural network (NN) analyses. Two perfect-match probes and 29 probes having a single-base-pair mismatch at positions 1 to 5 from the 5' terminus of the probe were designed to target one of two short sequences representing 16S rRNA. Nonequilibrium dissociation rates (i.e., melting profiles) of all probe-target duplexes were determined simultaneously. Analysis of variance revealed that position of the mismatch, type of mismatch, and formamide concentration significantly affected the T(d) and signal intensity. …
Type-Specific Inositol 1,4,5-Trisphosphate Receptor Localization In The Vomeronasal Organ And Its Interaction With A Transient Receptor Potential Channel, Trpc2, Jessica H. Brann, John C. Dennis, Edward E. Morrison, Debra A. Fadool
Type-Specific Inositol 1,4,5-Trisphosphate Receptor Localization In The Vomeronasal Organ And Its Interaction With A Transient Receptor Potential Channel, Trpc2, Jessica H. Brann, John C. Dennis, Edward E. Morrison, Debra A. Fadool
Biology: Faculty Publications and Other Works
The vomeronasal organ (VNO) is the receptor portion of the accessory olfactory system and transduces chemical cues that identify social hierarchy, reproductive status, conspecifics and prey. Signal transduction in VNO neurons is apparently accomplished via an inositol 1,4,5-trisphosphate (IP3)-activated calcium conductance that includes a different set of G proteins than those identified in vertebrate olfactory sensory neurons. We used immunohistochemical (IHC) and SDS-PAGE/western analysis to localize three IP3 receptors (IP3R) in the rat VNO epithelium. Type-I IP3R expression was weak or absent. Antisera for type-II and -III IP3R recognized appropriate molecular weight proteins by SDS-PAGE, and labeled protein could be …
Patch-Clamp Analysis Of Voltage-Activated And Chemically Activated Currents In The Vomeronasal Organ Of Sternotherus Odoratus (Stinkpot/Musk Turtle), D. A. Fadool, M. Wachowiak, J. H. Brann
Patch-Clamp Analysis Of Voltage-Activated And Chemically Activated Currents In The Vomeronasal Organ Of Sternotherus Odoratus (Stinkpot/Musk Turtle), D. A. Fadool, M. Wachowiak, J. H. Brann
Biology: Faculty Publications and Other Works
The electrophysiological basis of chemical communication in the specialized olfactory division of the vomeronasal (VN) organ is poorly understood. In total, 198 patch-clamp recordings were made from 42 animals (Sternotherus odoratus, the stinkpot/musk turtle) to study the electrically and chemically activated properties of VN neurons. The introduction of tetramethylrhodamine-conjugated dextran into the VN orifice permitted good visualization of the vomeronasal neural epithelium prior to dissociating it into single neurons. Basic electrical properties of the neurons were measured (resting potential, -54.5 +/- 2.7 mV, N=11; input resistance, 6.7 +/- 1.4 G Omega, N=25; capacitance, 4.2 +/- 0.3 pF, N=22; means +/- …
Isolation And Characterization Of A Genomic Clone For A Nitrate Reductasae Isoform From Soybean, Glycine Max, Brent Chyna
Isolation And Characterization Of A Genomic Clone For A Nitrate Reductasae Isoform From Soybean, Glycine Max, Brent Chyna
Master's Theses
No abstract provided.
Sequence Characterization And Phylogenetic Analyses Of The Pol Region From Sire-1, An Endogenous Retrovirus From Glycine Max, Eric A. Gaucher
Sequence Characterization And Phylogenetic Analyses Of The Pol Region From Sire-1, An Endogenous Retrovirus From Glycine Max, Eric A. Gaucher
Master's Theses
No abstract provided.
Adaptation Of The Freshwater Benthic Diatom Achanthidium Rostratum To Resource Limitation, Patrick Daniel Donovan
Adaptation Of The Freshwater Benthic Diatom Achanthidium Rostratum To Resource Limitation, Patrick Daniel Donovan
Dissertations
No abstract provided.
The Soybean Retroelement, Sire-1, Encodes An Envelope-Like Protein-Sire-1 Is An Endogenous, Proretrovirus-Like Genomic Element, Arpita Majumdar
The Soybean Retroelement, Sire-1, Encodes An Envelope-Like Protein-Sire-1 Is An Endogenous, Proretrovirus-Like Genomic Element, Arpita Majumdar
Master's Theses
No abstract provided.
Analysis Of The Inheritance Repression Of A Transposable Element In Drosophila Melanogaster, Melissa Leone
Analysis Of The Inheritance Repression Of A Transposable Element In Drosophila Melanogaster, Melissa Leone
Master's Theses
No abstract provided.
Benthic Invertebrate Community Responses To Round Goby (Neogobius Melanostomus) And Zebra Mussel (Dreissena Polymorpha) Invasion In Southern Lake Michigan, Linda A. Benning
Benthic Invertebrate Community Responses To Round Goby (Neogobius Melanostomus) And Zebra Mussel (Dreissena Polymorpha) Invasion In Southern Lake Michigan, Linda A. Benning
Master's Theses
No abstract provided.
Characterization Of Deletion Sites In A Human Collagen-Like Locus, Marissa A. Michaels
Characterization Of Deletion Sites In A Human Collagen-Like Locus, Marissa A. Michaels
Master's Theses
No abstract provided.
Regulation Of The Activities Of The Three Nitrate Reductase Isoforms In Wild-Type Soybean Glycine Max (L.) Merr. (Variety Williams) By Protein Phosphorylation, Maribel T. Joson
Regulation Of The Activities Of The Three Nitrate Reductase Isoforms In Wild-Type Soybean Glycine Max (L.) Merr. (Variety Williams) By Protein Phosphorylation, Maribel T. Joson
Master's Theses
No abstract provided.
Saccharomyces Cerevisiae-Produced Recombinant Pfs230: A Malaria Transmission-Blocking Vaccine Candidate, Kathryn M. Glynn
Saccharomyces Cerevisiae-Produced Recombinant Pfs230: A Malaria Transmission-Blocking Vaccine Candidate, Kathryn M. Glynn
Master's Theses
No abstract provided.
Afferent And Efferent Connections Of The Octavolateral Cerebellum In The Channel Catfish, Ictalurus Punctatus, Richard C. Rayborn
Afferent And Efferent Connections Of The Octavolateral Cerebellum In The Channel Catfish, Ictalurus Punctatus, Richard C. Rayborn
Master's Theses
No abstract provided.
The Distribution Of Gamma-Aminobutyric Acid (Gaba) And Glutamate In The Electrosensory System Of The Channel Catfish, Ictalurus Punctatus, Christian M. Fay
The Distribution Of Gamma-Aminobutyric Acid (Gaba) And Glutamate In The Electrosensory System Of The Channel Catfish, Ictalurus Punctatus, Christian M. Fay
Master's Theses
No abstract provided.
The Effects Of High Colony Density On Filtration, Mortality And Behavior In The Zebra Mussel, Dreissena Polymorpha, Christopher A. Call
The Effects Of High Colony Density On Filtration, Mortality And Behavior In The Zebra Mussel, Dreissena Polymorpha, Christopher A. Call
Master's Theses
No abstract provided.
Density Dependent Limitations And Vertical Position Of Diatoms In A Periphyton Mat, Ronald E. Johnson
Density Dependent Limitations And Vertical Position Of Diatoms In A Periphyton Mat, Ronald E. Johnson
Master's Theses
No abstract provided.
Sculpins As Predators Of Lake Trout Eggs And Larvae: An Assessment Of Their Predatory Capabilities And Constraints, Heather C. Biga
Sculpins As Predators Of Lake Trout Eggs And Larvae: An Assessment Of Their Predatory Capabilities And Constraints, Heather C. Biga
Master's Theses
No abstract provided.
The Role Of Protein Kinases In The Regulation Of The Na+/H+ Antiporter In Xenopus Oocytes, Andre P. J. Kulisz
The Role Of Protein Kinases In The Regulation Of The Na+/H+ Antiporter In Xenopus Oocytes, Andre P. J. Kulisz
Master's Theses
No abstract provided.
Microglial Reactivity To N-Methyl-D-Aspartate (Nmda) Induced Excitotoxicity In The Immature Rat Brain, Laia Acarin
Microglial Reactivity To N-Methyl-D-Aspartate (Nmda) Induced Excitotoxicity In The Immature Rat Brain, Laia Acarin
Master's Theses
No abstract provided.
Secondary Production And Dietary Preference In Orconectes Propinquus (Girard), Patricia B. Krema
Secondary Production And Dietary Preference In Orconectes Propinquus (Girard), Patricia B. Krema
Master's Theses
No abstract provided.
Relationship Between Bcl-2 Oncogene Expression And Apoptosis In A Human Leukemia T Cell Line, Sascha Hedjbeli
Relationship Between Bcl-2 Oncogene Expression And Apoptosis In A Human Leukemia T Cell Line, Sascha Hedjbeli
Master's Theses
No abstract provided.
Topography Of Anterior Lateral Line Nerve Branches In The Electrosensory And Mechanosensory Lateral Line Nuclei Of The Channel Catfish, Ictalurus Punctatus, Sanjay Singh
Master's Theses
No abstract provided.
Pathways Of Intracellular Protein Turnover In Cultured Human Fibroblasts, Sarah Olenick
Pathways Of Intracellular Protein Turnover In Cultured Human Fibroblasts, Sarah Olenick
Dissertations
No abstract provided.
A Comparison Of Insulin/Igf 1 And Progesterone-Induced Meiotic Maturation Of Xenopus Laevis Oocytes, Kevin T. Coleman
A Comparison Of Insulin/Igf 1 And Progesterone-Induced Meiotic Maturation Of Xenopus Laevis Oocytes, Kevin T. Coleman
Master's Theses
No abstract provided.
Cloning And Characterization Of A New Human Collagen Locus, Sheryl A. Cammarata
Cloning And Characterization Of A New Human Collagen Locus, Sheryl A. Cammarata
Master's Theses
No abstract provided.
An Evaluation Of Factors Affecting The Estrous Cycle Of The Djungarian Hamster, Phodopus Sungorus, Roberta W. Ellington
An Evaluation Of Factors Affecting The Estrous Cycle Of The Djungarian Hamster, Phodopus Sungorus, Roberta W. Ellington
Master's Theses
No abstract provided.
A Physical Map Of The Short Arm/Centromere Region Of Human Chromosome 21, Jennifer A. Doll
A Physical Map Of The Short Arm/Centromere Region Of Human Chromosome 21, Jennifer A. Doll
Master's Theses
No abstract provided.
Circulating Plasma Lipoproteins And Hepatic Ultrastructure In The Hibernating Thirteen-Lined Ground Squirrel, Spermophilus Tridecemlineatus, David G. Grecek
Circulating Plasma Lipoproteins And Hepatic Ultrastructure In The Hibernating Thirteen-Lined Ground Squirrel, Spermophilus Tridecemlineatus, David G. Grecek
Master's Theses
No abstract provided.