Biology Commons^™

Beneficial Effects Of Estradiol Are Mediated Through Apoe, Aseem Hussain

Masters Theses

Estradiol is the primary premenopausal hormone circulating in women. It is a powerful hormone in the female body since it sends signals to the brain, regulates ovulation and menstrual cycle and acts on the nervous syste~ heart, breast and many other tissues in the body. However all estradiol production is stopped after menopause. Post menopausal women can develop a host of neurodegenerative diseases such as Alzheimer's disease. If the onset of progressive dementia is after menopause during loss of estradiol, it seems logical to assume that the lack of estradiol should have a big role to play in the pathogenesis …

Tracing Sperm In Multiply-Mated Female Anastrepha Suspensa, (Diptera:Tephridae), Preeti Dhakal

Masters Theses

Anastrepha suspensa females have four different sites of sperm storage in their reproductive tract, may mate with more than one male, and store sperm in differing amounts in each organ after copulation. In order to study the dynamics of sperm storage patterns and subsequent paternity outcomes, a method was developed for both genotyping and quantifying sperm in the spermathecae. Spermathecae were isolated and sonicated to remove maternal cells, and subsequently crushed between two microslides to release sperm from the capsule. After DNA isolation, microsatellite loci could be amplified by PCR for fragment analysis. A newly developed method was then used …

Artificial Riffles As A Stream Remediation Technique, Bethany S. Harrington

Masters Theses

No abstract provided.

Influence Of Seed Source On Vegetative And Reproductive Performance Of Three Common Prairie Grasses Common In Grassland Restoration, Matt Burmeister

Masters Theses

Though tallgrass prairie once covered 250 million acres of the midwestem U.S, now less than one percent· of this original area is left. In recent years the restoration and reconstruction of tallgrass prairie has become more popular throughout the prairie region. There is evidence that local and distant populations may both be practical seed sources for ecological restoration projects. To address this basic issue in restoration, I tested the how performance of three grass species from different locations was affected by the distance from the restored area. Three species, Andropogon gerardii, Sorghastrum nutans and Panicum virgatum, of perennial grasses common …

Influence Of Fluvial Geomorphology On Fish Assemblage Structure Within An Agriculturally Impacted Watershed, Jeffrey D. Fore

Masters Theses

Warmwater stream fish assemblages are often considered limited by instream habitat availability. Thus, much current research has focused on determining habitat features that limit the abundance and persistence of stream fishes. These studies provide valuable information to managers who are concerned with increasing species abundance and persistence, but they may not provide information on the processes structuring the stream fish community. To address this issue, we hypothesized that stream geomorphology differed among stream sizes and types (i.e., channelized vs. unchannelized streams) and that the fish assemblages associated with each geomorphic type would be unique. Fish assemblages were sampled and geomorphic …

Long-Term Functional Trait Dynamics In Abandoned Agricultural Fields, Timothy A. Rye

Masters Theses

A fundamental question of plant succession is whether species within different successional stages differ in terms of functional traits as changes in community-level traits reflect changes in plant strategies over time. Although there are many studies that focus on that question, researchers have not studied the long-term dynamics of those traits within plant communities in depth. We used data from a 49-year study of old-field succession (the Buell-Small Succession Study) to assess the dynamics of important functional traits (height, seed mass, specific leaf area) and compared their dynamics among life forms and between native and non-native species. We assessed communitylevel …

Foraging Site Selection In Prairie Kingsnakes (Lampropeltis C. Calligaster): An Experimental Approach, Angela M. Fornell

Masters Theses

A critical component to a predator’s survival is the choice of foraging sites that yield enough predatory success to meet energetic demands. To elucidate cues used to select foraging sites, I examined foraging site selection in Prairie Kingsnakes (Lampropeltis c. calligaster), a generalist species whose foraging mode is flexible. My objectives were to determine: the pattern of foraging site selection; the importance of certain cues that kingsnakes use when selecting foraging sites; and, whether or not kingsnakes adjust their foraging patterns in response to alterations in the microhabitat. I tested subjects in a large experimental arena under several treatment conditions …

Terrestrial Macrofungi Of Old-Growth Prairie Groves, Vincent P. Hustad

Masters Theses

The purpose of this study was to evaluate and contrast the communities of terrestrial macrofungi of Brownfield and Trelease Woods, Champaign Co., IL; old-growth prairie groves isolated for> 120 years. This study represents the first known analysis of fungal communities in old-growth prairie grove ecosystems in the Midwestern United States, and provides an excellent opportunity to characterize the fungal biodiversity of established old-growth reference communities for comparisons with other sites, both local and distant. Fungal communities were sampled during the fall fruiting seasons of 2006 and 2007 using a plot frequency study of fungal abundance. Several estimators of species richness …

Correlating Phytoplankton Assemblages With Water Quality In Illinois Lakes And Reservoirs: Validating Models Based On Historical Data, Jason D. Allen

Masters Theses

In the last two decades management and monitoring efforts in freshwater systems has shifted from point source pollutants to non-point source pollutants and environmental modification. Long term, large scale sampling projects necessitated by such a shift often are infeasible due to logistical, cost, and time constraints.

In recent years fish and benthic macroinvertebrate indices of biological integrity (IBI's) have been used to survey the quality of flowing (lotic) water systems, which have drastically reduced sampling time and facilitated large scale monitoring projects. However, it is unlikely that these could be adapted to lentic systems. This study was undertaken in an …

The Impact Of Climate On The Leaf Characteristics Of Genus Fraxinus, Brian W. Craven

Masters Theses

Fraxinus (ash) has six species across eastern North America, five of which are found in Illinois (F. americana, F. pennsylvanica, F. nigra, F. quadrangulata, F. profimda). It is also threatened by the emerald ash borer (Agrilus planipennis) (BAB). Are climate differences across a north-south distance of 600 km great enough to create differences in the morphological characteristics of Fraxinus species? Studying latitudinal variation in the species of Fraxinus would reveal the impact of climate on the leaf characteristics of a genus of trees which extend over a considerable portion of the continent. The objectives of this study were to determine …

Evaluation Of The Oxalate-Consuming Activities Of Probiotic Microorganisms, Alexandra E.C. Baluka

Masters Theses

This study identifies the oxalate-consuming capabilities of mixtures of probiotic microorganisms, present as commercial probiotics, as well as in seventeen pure cultures of probiotic microorganisms. Probiotics are sold commercially as powders, tablets, and supplements of live microorganisms that, when taken in large amounts, confer a health benefit to the host. Increased levels of urinary oxalate coincide with the formation of kidney stones, so this study sought to identify organisms capable of oxalate consumption that could potentially be manufactured for those suffering from calcium oxalate kidney stones. This study determined that consumption of oxalate was not directly tied to active growth …

Mating Bias In The Red Imported Fire Ant, Solenopsis Invicta, Kendra Carroll

Masters Theses

The two social forms in the red imported fire ant, Solenopsis invicta, are believed to be governed by the expression of one specific gene. This gene, known as Gp-9, is responsible for the production of general protein-9, thought to be a pheromone binding protein. Among the social aspects thought to be controlled by this locus is reproduction. Many aspects of breeding within and between the two colonial forms have been investigated over the past years since in introduction of this species to the United States. In this study, several features of the currently accepted model were examined. Newlymated queens were …

Evaluating The Illinois Stream Valley Segment Model As An Effective Management Tool, Stephen S. Warrner

Masters Theses

Stream habitat assessment procedures have been used to monitor logical potential, habitat restoration, and determine anthropogenic impacts on streams. In the past, the Illinois Department of Natural Resources (IDNR) and the Illinois Natural History Survey (INHS) have determined which stream reaches to sample habitat quality based on historical data, accessibility, and whether or not a reach is representative of an area. To develop a consistent and unbiased procedure for choosing sampling locations, the IDNR and INHS have proposed a technique by which watersheds are divided into homogeneous stream segments called valley segments. Valley segments within each watershed are determined by …

Spatial And Ecological Patterns Of Tent Location In The Eastern Tent Caterpillar, Malacosoma Americana (Lepidoptera:Lasiocampidae), Jennifer Pleasant

Masters Theses

The eastern tent caterpillar, Malacosoma americanum (Fabricius) (Lepidoptera: Lasiocampidae) is a widely studied social caterpillar. The nests (i.e. tents) of the tent caterpillar are most frequently found on Black Cherry trees (Prunus serotina). However, dispersions of tents are often clumped with one cherry tree having five or more tent caterpillar nests in it and another cherry tree 3 meters away having only one nest, or none at all. I tried to determine why this happens. Because of the short field season, much of the research on the caterpillars has been done in the laboratory. I tried to determine host selection …

A Survey Of Carrion And Burying Beetles (Coleoptera:Silphidae) In Mchenry County, Illinois, David Suhrbur

Masters Theses

This study compared the occurrence of beetles in the family Silphidae using historical collection records with that of field collections from five different sites within McHenry County. The five sites include: Glacial Park, Woodstock, Silver Creek, Rush Creek, and Coral Woods. Historically, 11 known silphid species have been found in McHenry County, compared with seven species found during this study' collection period.

Percentages of silphid species at each site was calculated, as well as total number of individuals sampled, and two indices of diversity (Shannon-Weiner and Simpson's) were applied to both current and historical collection data. The historical collection (when …

Dessication Stress, Steven C. Hand, Michael A. Menze

Faculty Research & Creative Activity

The threat of desiccation for organisms inhabiting the intertidal zone occurs during emersion at low tides or when organisms are positioned in the high intertidal zone, where wetting occurs primarily by spring tides, storm waves, and spray. Drying due to evaporative water loss is the most common mechanism for dehydration, although during winter in northern temperate regions freezing can also occur, which reduces the liquid water in extracellular fluids and can lead to intracellular dehydration in multicellular organisms. Freezing tolerance has been reported and characterized for a number of intertidal invertebrates, including gastropods such as an air-breathing snail and a …

Floral Biology Of Physaria Ludoviciana (Brassicaceae), A Plant Rare To The Midwest, Ann E. Claerbout, Janice M. Coons, Henry R. Owen, Kenneth R. Robertson

Faculty Research & Creative Activity

Physaria ludoviciana (Brassicaceae) is rare in Illinois, Minnesota, and Wisconsin. Environmental effects on floral development are unclear. Both self-compatibility and self-incompatibility occur within Physaria species. Objectives were to describe flowers, to determine how photoperiod affects flower development, and to predict whether flowers are self-compatible or self-incompatible. For photoperiods, greenhouse-grown plants were placed in either 16 or 8 hr photoperiods. Inflorescences and open flowers were counted weekly. For pollination, flowers were self-pollinated or cross-pollinated. Plants developed inflorescences after 20 and 28 d in long and short days, respectively. Inflorescences/plant increased for both photoperiods throughout the study. In short days, plants produced …

The Distribution Of Apolipoprotein E In Mouse Olfactory Epithelium, Britto Nathan, Sreenivas Nannapaneni, Salina Gairhe, Ikemefuna Nwosu, Robert Struble

Faculty Research & Creative Activity

Previous studies from our laboratory suggest that apolipoprotein (apoE), a lipid transporting protein, facilitates olfactory nerve regeneration. We have shown that apoE is enriched in the olfactory nerve and around the glomeruli of the olfactory bulb (OB). The studies reported herein were undertaken to identify possible sources of apoE in the olfactory epithelium (OE). Immunoblotting results revealed apoE expression in the OE of wild-type (WT) mice, but not in apoE deficient/knockout (KO) mice. Immunohistochemical studies revealed that the perikarya and processes of sustentacular (Sus) cells expressed apoE-like immunoreactivity. Minimal neuronal apoE immunostaining was seen, although apoE was observed in the …

The Distribution Of Apolipoprotein E In Mouse Olfactory Epithelium, Britto P. Nathan, Sreenivas Nannapaneni, Salina Gairhe, Ikemefuna Nwosu, Robert G. Struble

Faculty Research & Creative Activity

Previous studies from our laboratory suggest that apolipoprotein (apoE), a lipid transporting protein, facilitates olfactory nerve regeneration. We have shown that apoE is enriched in the olfactory nerve and around the glomeruli of the olfactory bulb (OB). The studies reported herein were undertaken to identify possible sources of apoE in the olfactory epithelium (OE). Immunoblotting results revealed apoE expression in the OE of wild-type (WT) mice, but not in apoE deficient/knockout (KO) mice. Immunohistochemical studies revealed that the perikarya and processes of sustentacular (Sus) cells expressed apoE-like immunoreactivity. Minimal neuronal apoE immunostaining was seen, although apoE was observed in the …

Physical Contests For Females In The Japanese Beetle, Popillia Japonica, Paul Switzer, Kipp Kruse

Faculty Research & Creative Activity

We conducted field observations of physical competition for mates, in which a single male attempts to usurp a female from another male, in male Japanese beetles, Popillia japonica Newman (Coleoptera; Scarabaeidae). Physical contests for mates were relatively rare, but when they occurred the challenger male was able to successfully takeover females by dislodging the previously paired resident male in only 18% of contests, suggesting that a substantial prior residency advantage exists in this species. Challenger males that were successful in takeover attempts were significantly larger than the resident male. In contrast, no size pattern was found between intruding males and …

Production Of Methanethiol And Volatile Sulfur Compounds By The Archaeon ‘‘Ferroplasma Acidarmanus’’, David J. Baumler, Kai F. Hung, Kwang Cheol Jeong, Charles W. Kaspar

Faculty Research & Creative Activity

Acidophiles are typically isolated from sulfaterich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown ‘‘Ferroplasma acidarmanus’’ showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/ MS determined that the volatile sulfur compound emitted by active ‘‘F. acidarmanus’’ cultures is methanethiol. In ‘‘F. acidarmanus’’ cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and …

Native And Exotic Plant Species Exhibit Similar Population Dynamics During Succession, Scott Meiners

Faculty Research & Creative Activity

A growing body of literature has led to the debate in invasion biology whether exotic species perform within communities differently than native taxa due to inherent advantages. To address this issue, the population dynamics of native and exotic plant species were assessed from a 48-year record of permanent plot data from the Hutcheson Memorial Forest Center (New Jersey, USA) to determine rate of increase, lag time, maximum frequency, and the year of peak frequency. Overall, native and exotic species exhibited very similar population dynamics. Rates of increase and length of lag times were similar between native and exotic taxa but …

Life Without Water: Expression Of Plant Lea Genes By An Anhydrobiotic Arthropod, Steven C. Hand, Dana Jones, Michael A. Menze, Trudy L. Witt

Faculty Research & Creative Activity

Anhydrobiotic animals protect cellular architecture and metabolic machinery in the dry state, yet the molecular repertoire supporting this profound dehydration tolerance is not fully understood. For the desiccation-tolerant crustacean, Artemia franciscana, we report differential expression of two distinct mRNAs encoding for proteins that share sequence similarities and structural features with late-embryogenesis abundant (LEA) proteins originally discovered in plants. Bioinformatic analyses support assignment of the LEA proteins from A. franciscana to group 3. This eucoelomate species is the most highly evolved animal for which LEA gene expression has been reported. It is becoming clear that an ensemble of micromolecules and macromolecules …

Native And Exotic Plant Species Exhibit Similar Population Dynamics During Succession, Scott J. Meiners

Faculty Research & Creative Activity

A growing body of literature has led to the debate in invasion biology whether exotic species perform within communities differently than native taxa due to inherent advantages. To address this issue, the population dynamics of native and exotic plant species were assessed from a 48-year record of permanent plot data from the Hutcheson Memorial Forest Center (New Jersey, USA) to determine rate of increase, lag time, maximum frequency, and the year of peak frequency. Overall, native and exotic species exhibited very similar population dynamics. Rates of increase and length of lag times were similar between native and exotic taxa but …

Alaska At The Crossroads Of Migration: Space-Based Ornithology, Jill L. Deppe, K Wessels, J A. Smith

Faculty Research & Creative Activity

Understanding bird migration on a global scale is one of the most compelling and challenging problems of modern biology. Each year multitudes of migratory birds travel between breeding grounds in Alaska and wintering grounds in the Americas, Asia, and Australia. Here we present the conceptual framework for a spatially explicit, individual-based biophysical migration model driven by dynamic remote sensing observations of atmospheric and land surface conditions to simulate migration routes, timing, energy budgets, and probability of survival. Understanding temporal and spatial patterns of bird migration will provide insight into pressing conservation and human health issues related to this taxonomic group.

Phylogeny And Geological History Of The Cynipoid Wasps (Hymenoptera: Cynipoidea), Zhiwei Liu, Michael S. Engel, David A. Grimaldi

Faculty Research & Creative Activity

The geological history of the wasp superfamily Cynipoidea is reviewed, with the description of various new taxa, being mostly in Late Cretaceous amber from New Jersey and Canada. The various fossil lineages are incorporated into a phylogenetic analysis of the superfamily, and their implications for understanding the evolution of the group are explored. The following new taxa or taxonomic changes are proposed (authorship of all taxa is Liu and Engel): Protimaspidae, new family; Stolamissidae, new family; Stolamissus, new genus; Stolamissus mirabilis, new species; Proliopterinae, new subfamily; Proliopteron, new genus; Proliopteron redactus, new species; Goeraniinae, new subfamily; Goerania, new genus; Goerania …

Over-Expression Of Udp-Glucose Pyrophosphorylase In Hybrid Poplar Affects Carbon Allocation, Heather Coleman, Thomas Canam, Kyu-Young Kang, David Ellis, Shawn Mansfield

Faculty Research & Creative Activity

The effects of the over-expression of the Acetobacter xylinum UDP-glucose pyrophosphorylase (UGPase) under the control of the tandem repeat Cauliflower Mosaic Virus promoter (2335S) on plant metabolism and growth were investigated in hybrid poplar (Populus alba3grandidentata). Transcript levels, enzyme activity, growth parameters, leaf morphology, structural and soluble carbohydrates, and soluble metabolite levels were quantified in both transgenic and wild-type trees. Transgenic 2335S::UGPase poplar showed impaired growth rates, displaying reduced height growth and stem diameter. Morphologically, 2335S::UGPase trees had elongated axial shoots, and leaves that were substantially smaller in size when compared with wild-type trees at equivalent developmental stages. Biochemical analysis …

Production Of Methanethiol And Volatile Sulfur Compounds By The Archaeon ‘‘Ferroplasma Acidarmanus’’, David Baumler, Kai Hung, Kwang Jeong, Charles Kaspar

Faculty Research & Creative Activity

Acidophiles are typically isolated from sulfaterich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown ‘‘Ferroplasma acidarmanus’’ showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/ MS determined that the volatile sulfur compound emitted by active ‘‘F. acidarmanus’’ cultures is methanethiol. In ‘‘F. acidarmanus’’ cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and …

Review Of Breeding Field Crops, 5th Ed., Henry R. Owen

Faculty Research & Creative Activity

No abstract provided.

Review Of Dictionary Of Plant Tissue Culture, Henry R. Owen

Faculty Research & Creative Activity

No abstract provided.