Biology Commons^™

Experimentally Altered Navigational Demands Induce Changes In The Cortical Forebrain Of Free-Ranging Northern Pacific Rattlesnakes (Crotalus O. Oreganus), Matthew L. Holding, Julius A. Frazier, Emily N. Taylor, Christine R. Strand

Biological Sciences

The hippocampus of birds and mammals plays a crucial role in spatial memory and navigation. The hippocampus exhibits plasticity in adulthood in response to diverse environmental factors associated with spatial demands placed on an animal. The medial and dorsal cortices of the telencephalon of squamate reptiles have been implicated as functional homologues to the hippocampus. This study sought to experimentally manipulate the navigational demands placed on free-ranging northern Pacific rattlesnakes (Crotalus o. oreganus) to provide direct evidence of the relationship between spatial demands and neuroplasticity in the cortical telencephalon of the squamate brain. Adult male rattlesnakes were radio-tracked for 2 …

The Proteomic Response Of Mytilus Californianus To Acute Oxidative Stress In The Presence Of A Sirtuin Inhibitor, H. Chilton, J. Mier, M. Zuzow, L. Tomanek

Biological Sciences

The ribbed mussel (Mytilus californianus) occurs in the in the rocky intertidal zone along the Pacific coast of North America where it experiences greatly varying environmental conditions in terms of temperature and hypoxia, stressors that are known to induce oxidative stress. It has been hypothesized that while under acute heat stress, related Mytilus congeners undergo a shift in redox potential through the reduction of NADH fueled respiration pathways to the production of the reducing agent NADPH as a potential defensive mechanism against the production of reactive oxygen species. In addition, it has been hypothesized that sirtuins (a family …

Proteomic Responses Of Mytilus Congeners To Hypo-Saline Stress, M. Z. Zuzow, L. Serafini, L. Hitt, J. Valenzuela, L. Tomanek

Biological Sciences

The invading Mediterranean blue mussel species Mytilus galloprovincialis requires higher salinity levels than the more hypo-saline tolerant and native M.trossulus,which it replaced from its southern range in California, possibly due to climate change.It is also the warm-adapted congener.Hypo-salinity,due to an increase in extreme precipitation events,has been hypothesized to limit the expansion of the invader.By comparing the proteomic responses of the congeners to acute hypo-saline stress we were trying to identify the interspecific differences that set limits to hypo-saline stress and assess their contribution to the range shifts.We exposed mussels to acute hypo-saline(100%, 85% and 70% salinity)stress for 4h followed …

Proteomic Analysis Of Hyposalinity Stress In The Ascidian Species Ciona Savignyi And C. Intestinalis, James S. Koman, Lars Tomanek

Biological Sciences

The ascidian species Ciona savignyi and C. intestinalis are invasive species but show interspecific differences in their population response to hypo-saline stress associated with heavy winter-run off events that are predicted to become more frequent due to climate change. Despite an almost world-wide distribution, C. intestinalis seems to be more susceptible to hypo-saline stress than the geographically more limited C. savignyi. Given that the genomes of both species are fully sequenced, we were able to compare their proteomic response to both acute and chronic salinity to characterize the mechanisms that are responsible for setting tolerance limits to hyposaline conditions …

Proteomic Response Of Tidal-Rhythm Entrained Mytilus Californianus To Acute Aerial Heat Stress, D. Hernandez, M. Schuman, L. Tomanek

Biological Sciences

The rocky intertidal mussel species Mytilus californianus is native to the Pacific coast of North America. It is frequently exposed to temperatures that can induce the cellular stress response due to the tidal rhythm. Instead of acclimating mussels to common conditions in the laboratory for several weeks, we collected mussels from a site with mussel body temperature recordings (specimens’ thermal history) and immediately started experimentation by mimicking the tidal rhythm with different levels of heat stress over multiple low tides and with different recovery periods. By using individuals that were entrained to the tidal rhythm and by maintaining it, we …

The Discovery Of Insulin: A Case Study Of Scientific Methodology, William D. Stansfield

Biological Sciences

The nature of scientific research sometimes involves a trial-and-error procedure. Popular reviews of successful results from this approach often sanitize the story by omitting unsuccessful trials, thus painting the rosy impression that research simply follows a direct route from hypothesis to experiment to scientific discovery. The discovery of insulin is a classical case study in this genre that begs for an explanation to our students because it is so often ignored or misrepresented even in biology and physiology textbooks.

Bioluminescence In The High Arctic During The Polar Night, J. Berge, A.S. Båtnes, G. Johnsen, S.M. Blackwell, Mark A. Moline

Biological Sciences

This study examines the composition and activity of the planktonic community during the polar night in the high Arctic Kongsfjord, Svalbard. Our results are the first published evidence of bioluminescence among zooplankton during the Arctic polar night. The observations were collected by a bathyphotometer detecting bioluminescence, integrated into an autonomous underwater vehicle, to determine the concentration and intensity of bioluminescent flashes as a function of time of day and depth. To further understand community dynamics and composition, plankton nets were used to collect organisms passing through the bathyphotometer along with traditional vertical net tows. Additionally, using a moored bathyphotometer closed …

New Metrics For Managing And Sustaining The Ocean’S Bounty, Heather Tallis, Sarah E. Lester, Mary Ruckelshaus, Mark Plummer, Karen Mcleod, Anne Guerry, Sandy Andelman, Margaret R. Caldwell, Marc Conte, Stephen Copps, David Fox, Rod Fujita, Steven D. Gaines, Guy Gelfenbaum, Barry Gold, Peter Kareiva, Choong-Ki Kim, Kai Lee, Michael Papenfus, Scott Redman, Brian Silliman, Lisa Wainger, Crow White

Biological Sciences

Policies are arising around the world, most recently in the United States, that mandate the implementation of marine spatial planning as a practical pathway towards ecosystem-based management. In the new United States ocean policy, and several other cases around the globe, ecosystem services are at the core of marine spatial planning, but there is little guidance on how ecosystem services should be measured, making it hard to implement this new approach. A new framework is shown here for practical, rigorous ecosystem service measurement that highlights contributions from both natural and social systems. The novel three-step framework addresses traditional shortcomings of …

Mycorrhizal Colonization Of Hypericum Perforatum L. (Hypericaceae) From Serpentine And Granite Outcrops On The Deer Isles, Maine, Naveed Davoodian, Jason Bosworth, Nishanta Rajakaruna

Biological Sciences

Given the paucity of literature on plant-fungal interactions on serpentine soils and limited investigation of serpentine geoecology in eastern North America, we examined mycorrhizal colonization of Hypericum perforatum from adjacent serpentine and granite outcrops on the Deer Isles, ME to determine whether plants were differentially colonized based on substrate. We coincided our sampling with three phenologic stages of H. perforatum (preflowering, flowering, postflowering) to determine possible differences in colonization based on plant phenology. The levels of mycorrhizal colonization in H. perforatum were not significantly different between serpentine and granite sites, while levels of colonization in postflowering plants were significantly higher …

A Collaborative Approach To Investigate Site Fidelity, Home Range, And Homing Behavior Of Cabezon (Scorpaenichthys Marmoratus), Carlos Mireles, Royden Nakamura, Dean Wendt

Biological Sciences

Understanding the area use requirements of species targeted for protection by marine protected areas (MPAs) is critical to the future conservation efforts of economically important fish species. Knowledge of home range size and site fidelity is essential in determining whether species will benefit from the protection offered by a MPA, the size of the area needed to protect individuals, and the extent to which surrounding unprotected areas may be supplemented through post-recruitment movement or “spillover”. We utilized a traditional mark and recapture approach, along with GIS spatial analysis to investigate the site fidelity, home range, and homing behavior of the …

Utilizing Fishermen Knowledge And Expertise: Keys To Success For Collaborative Fisheries Research, Noëlle Yochum, Richard M. Starr, Dean Wendt

Biological Sciences

Collaborative fisheries research provides a mechanism for integrating the unique knowledge, experience, and skills of fishermen and scientists. It is a joint intellectual endeavor that begins with the inception of a project and continues until its final stages, with each group having mutual investment in—and ownership of— the project. Collaborative fisheries research promotes communication and trust among fishermen, scientists, and managers and can provide much-needed scientifically valid data for fisheries management. It can enhance federal and state management data collection programs, which span broad sections of coastline, by increasing the ability to detect changes in local metapopulations that may be …

Traversing Swanton Road, 6th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

Parasite Infection And Sand Coarseness Increase Sand Crab (Emerita Analoga) Burrowing Time, Gita R. Kolluru, Zachary S. Green, Larisa K. Vredevoe, Matthew R. Kuzma, Sera N. Ramadan, Marc R. Zosky

Biological Sciences

Parasites with indirect life cycles require trophic transmission from intermediate hosts to definitive (vertebrate) hosts. Transmission may be facilitated if parasite infection alters the behavior of intermediate hosts such that they are more vulnerable to predation. Vulnerability to predation may also be influenced by abiotic factors; however, rarely are the effects of parasites and abiotic factors examined simultaneously. The swash zone of sandy beaches is a particularly harsh environment. Sand crabs (Emerita analoga) burrow rapidly in the swash zone to avoid predators and dislodgment. We examined prevalence and abundance of the acanthocephalan parasite Profilicollis altmani in sand crabs, …

Climate Change, Body Size, And Phenotype Dependent Dispersal, Shannon J. Mccauley, Karen E. Mabry

Biological Sciences

No abstract provided.

Investigating The Roles Of Ndj1 And Tid1 In Crossover Assurance In Saccharomyces Cerevisiae, Rianna Knowles

Master's Theses

Meiosis is the specialized process of cell division utilized during gametogenesis in all sexually reproducing eukaryotes, which consists of one round of DNA replication followed by two rounds of chromosome segregation and results in four haploid cells. Crossovers between homologous chromosomes promote proper alignment and segregation of chromosomes during meiosis.

Crossover interference is a genetic phenomenon in which crossovers are non-randomly placed along chromosomes. Crossover assurance ensures that every homologous chromosome pair obtains at least one crossover during Prophase I. Crossovers physically connect homologous pairs, allowing spindle fibers to attach and separate homologs properly. However, some organisms have shown an …

Educational Curriculum Standards & Standardized Educational Tests: Comparing Apples & Oranges?, William D. Stansfield

Biological Sciences

Mandated biology curriculum standards and standardized tests may vary widely from one state to another, making academic-performance comparisons among the states problematic. This report outlines the effects of the “No Child Left Behind” law and uses California as a test case against which teachers in other states may make comparisons of their own biology curriculum standards and tests. Several sources are cited that offer sample multiple-choice questions that have appeared on previous California standardized tests for sciences/life sciences/biology. These examples, and critique thereof, may help teachers better prepare their own tests to improve student performance in meeting the state's curriculum …

Season And Sex Of Host Affect Intensities Of Ectoparasites In Western Fence Lizards (Sceloporus Occidentalis) On The Central Coast Of California, Anthony S. Lumbad, Larisa K. Vredevoe, Emily N. Taylor

Biological Sciences

Seasonal variability in intensities of ectoparasites of western fence lizards (Sceloporus occidentalis) was quantified in spring, summer, and autumn 2008. Lizards were suspended over pans of water in the laboratory to enumerate replete western black-legged ticks (Ixodes pacificus) and mites. Intensity of ectoparasites was greatest in spring. On average, mites comprised the greatest proportion of ectoparasites, followed by larval and nymphal ticks, respectively. Mites were on lizards year-round. Overall intensity of ticks was greatest in spring, but low during summer and autumn. Males were more heavily parasitized by ticks than females in spring, whereas females had …

The Proteomic Response Of Sea Squirts (Genus Ciona) To Acute Heat Stress: A Global Perspective On The Thermal Stability Of Proteins, Loredana Serafini, Jay B. Hann, Dietmar Kültz, Lars Tomanek

Biological Sciences

Congeners belonging to the genus Ciona have disparate distributions limited by temperature. Ciona intestinalis is more widespread with a cosmopolitan distribution ranging from tropical to sub-arctic zones, while Ciona savignyi is limited to temperate-latitudes of the northern Pacific Ocean. To compare the heat stress response between congeners, we quantified changes in protein expression using proteomics. Animals were exposed to 22 °C, 25 °C, and 28 °C for 6 h, then recovered at a control temperature (13 °C) for 16 h (high heat stress experiment). In a second experiment we exposed animals to lower levels of heat stress at 18 °C, …

Interspecies Comparison Of Αii-Spectrin Abundance Between Chinook Salmon And Steelhead, Brielle D. Kemis, Ann L. Miracle, Katie A. Wagner, Christa M. Woodley

STAR Program Research Presentations

Salmonids, such as Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss), are a staple economic, recreational, tribal, and environmental resource, yet many populations are unsustainable. This study was part of a broad scale effort to monitor the impact of downstream migration obstacles on juvenile salmonid health and survival, which is an essential step towards increasing Smolt-to-Adult Return ratios (SARs). The objective of this study was to determine if juvenile Chinook salmon and steelhead exhibit differing quantities of alphaII-Spectrin Breakdown Products (SBDPs) over two consecutive spring migration periods, indicative of neurogenesis rate and/or biological response to head …

Forward Osmosis Bag: Ground Testing The Prospect Of Using Alternative Water Sources For Drinking Water, Michaela Marie Brant, Ariella Yendler, Mona Hammoudeh, Michael T. Flynn

STAR Program Research Presentations

On the STS-135 Space Shuttle mission, to be launched July 8, 2011, a forward osmosis bag (FOB) study will be conducted. At NASA Ames this summer, the ground truth testing is being conducted for results comparisons. The FOB technology is derived from a commercial product, the X-Pack water filter. Forward osmosis operates by utilizing an established concentration gradient across a semi-permeable membrane to move water molecules from one side of the membrane to the other. This concept is exploited to harvest drinking water from grey water sources such as urine, sea water, or vehicle water. In this experiment, potassium chloride …

Addressing Biased Occurrence Data In Predicting Potential Sierra Nevada Red Fox Habitat For Survey Prioritization, Casey Cleve, John D. Perrine, Barbara Holzman, Ellen Hines

Biological Sciences

The Sierra Nevada red fox Vulpes vulpes necator is listed as a threatened species under the California Endangered Species Act. It originally occurred throughout California’s Cascade and Sierra Nevada mountain regions. Its current distribution is unknown but should be determined in order to guide management actions. We used occurrence data from the only known population, in the Lassen Peak region of northern California, combined with climatic and remotely sensed variables, to predict the species’ potential distribution throughout its historic range. These model predictions can guide future surveys to locate additional fox populations. Moreover, they allow us to compare the relative …

Bridging The Marine-Terrestrial Disconnect To Improve Marine Coastal Zone Science And Management, Benjamin I. Ruttenberg, Elise F. Granek

Biological Sciences

Coastal zone ecosystems sit between larger terrestrial and marine environments and, therefore, are strongly affected by processes occurring in both systems. Marine coastal zone systems provide a range of benefits to humans, and yet many have been significantly degraded as a result of direct and indirect human impacts. Management efforts have been hampered by disconnects both between management and scientific research and across linked marine–terrestrial systems. Management jurisdictions often start or end at the shoreline, and multiple agencies at different levels of government often have overlapping or conflicting management goals or priorities, or suffer from a lack of knowledge or …

Tissue-Specific Thyroid Hormone Regulation Of Gene Transcripts Encoding Iodothyronine Deiodinases And Thyroid Hormone Receptors In Striped Parrotfish (Scarus Iseri), Kaitlin M. Johnson, Sean C. Lema

Biological Sciences

In fish as in other vertebrates, the diverse functions of thyroid hormones are mediated at the peripheral tissue level through iodothyronine deiodinase (dio) enzymes and thyroid hormone receptor (tr) proteins. In this study, we examined thyroid hormone regulation of mRNAs encoding the three deiodinases dio1, dio2 and dio3 – as well as three thyroid hormone receptors trαA, trαB and trβ – in initial phase striped parrotfish (Scarus iseri). Parrotfish were treated with dissolved phase T₃ (20 nM) or methimazole (3 mM) for 3 days. Treatment with exogenous T₃ elevated circulating T₃, while the …

A Preliminary Study Of The Vegetation Of Vernal Pools Of Acadia National Park, Maine, U.S.A, Brett Ciccotelli, Tanner B. Harris, Bruce Connery, Nishanta Rajakaruna

Biological Sciences

We conducted a preliminary floristic study of six vernal pools in Acadia National Park on Mount Desert Island, Maine. Plant species were recorded on three sampling dates from April to October, 2008. Sixty-five vascular plant species from 26 families were recorded. Of these, 27 are considered occasional or uncommon in Acadia National Park. Thirteen species are new reports for vernal pools in the northeastern United States. This represents the first published study of the vernal pool flora of Acadia National Park.

Predator-Induced Demographic Shifts In Coral Reef Fish Assemblages, Benjamin I. Ruttenberg, Scott L. Hamilton, Sheila M. Walsh, Mary K. Donovan, Alan Friedlander, Edward Demartini, Enric Sala, Stuart A. Sandin

Biological Sciences

In recent years, it has become apparent that human impacts have altered community structure in coastal and marine ecosystems worldwide. Of these, fishing is one of the most pervasive, and a growing body of work suggests that fishing can have strong effects on the ecology of target species, especially top predators. However, the effects of removing top predators on lower trophic groups of prey fishes are less clear, particularly in highly diverse and trophically complex coral reef ecosystems. We examined patterns of abundance, size structure, and age-based demography through surveys and collection-based studies of five fish species from a variety …

Camera Study For Mammalian Carnivore Presence Across Seasons In Montana De Oro State Park, San Luis Obispo County, Ca, Samuel R. Young

Natural Resources Management and Environmental Sciences

Montana de Oro (MDO) is one of the largest state parks in California. Little is known however about its wildlife or their habits. Large predators such as mountain lion (Puma concolor) and black bear (Ursus americanus), and non–native species such as red fox (Vulpes vulpes) and feral pig (Sus scropha) are of management concern because of their potentially dramatic ecological roles. Cameras were deployed at 5 sites along the Coon Creek hiking trail in spring (May 3–June 7), summer (July 15–August 19), and fall (October 10–November 23) sessions with the goal of …

Using Portfolio Theory To Assess Tradeoffs Between Return From Natural Capital And Social Equity Across Space, Benjamin S. Halpern, Crow White, Sarah E. Lester, Christopher Costello, Steven D. Gaines

Biological Sciences

Spatial variance in returns from natural resources, driven by resource dynamics and regulations, can have strong consequences for equitable delivery of value to individuals and communities. Yet resource management models implicitly weight returns equally across space, even when space is explicitly included in model dynamics and policy. Here we translate financial portfolio theory from the temporal to spatial realm and use it to quantify the inherent tradeoff between resource returns and social equity, defined as a more uniform distribution of resource value across space. We illustrate this approach with a marine case study of the Channel Islands, California, USA. Depending …

Distinct Microbiome In Pouchitis Compared To Healthy Pouches In Ulcerative Colitis And Familial Adenomatous Polyposis, Garrett C. Zella, Elizabeth J. Hait, Tiffany Glavan, Dirk Gevers, Doyle V. Ward, Christopher L. Kitts, Joshua R. Korzenik

Biological Sciences

Background: Pouchitis occurs in up to 50% of patients with ulcerative colitis (UC) undergoing ileal pouch anal anastomosis (IPAA). Pouchitis rarely occurs in patients with familial adenomatous polyposis (FAP) who undergo IPAA. Our aim was to compare mucosal and luminal flora in patients with UC-associated pouchitis (UCP), healthy UC pouches (HUC), and healthy FAP pouches (FAP).

Methods: Nineteen patients were enrolled in thi s cross-sectional study (nine UCP, tl1ree HUC, seven FAP). Patie nts with active pouchitis were identified using the Pouchitis Disease Activity Index (PDAI). Ileal pouch mucosal biopsies and fecal samples were analyzed with a 16S rDNA-based terminal …

Traversing Swanton Road, 5th Ed., James A. West

Academic, Government & Associated Organizational Research Resources

Situated at the northwest end of Santa Cruz County and occupying circa 30 square miles of sharply contrasted terrain, the Scott Creek Watershed concentrates within its geomorphological boundaries, at least 10-12% of California's flora, both native and introduced. Paradoxically, the use/abuse that the watershed has sustained over the past 140+ years, has not necessarily diminished the biodiversity and perhaps parallels the naturally disruptive but biologically energizing processes (fire, flooding, landslides and erosion), which have also been historically documented for the area for +60 years. This is an early edition of an extensive document by James A. West. Please visit the …

The Truth About Models: How Well Do Mechanical Models Mimic The Observed Gender Distributions In Two-Child Families?, William D. Stansfield, Matthew A. Carlton

Biological Sciences

We question the use of mechanical models, such as coin flipping, to represent the probabilities of gender distributions in sibship families consisting of two children. Both the assumptions of the models and the reliability of the data should be evaluated. Using models without these critical evaluations may tend to perpetuate myths rather than elucidate biological realities.