Biology Commons^™

An Observation Of Apparent Teaching Behavior In The Pallid Bat, Antrozous Pallidus, Jessie P. Bunkley, Jesse R. Barber

Biology Faculty Publications and Presentations

During a laboratory study of pallid bat (Antrozous pallidus) hunting behavior, we observed an interaction wherein an adult female appeared to aid a juvenile male in learning a novel foraging task. This single observation adheres to the 3 requirements of teaching outlined by Caro and Hauser (1992). A female bat experienced with a hunting task modified her behavior in the presence of a naïve observing male, resulting in a cost of reduced food availability to the female when she was hungry, while directing the male to food resources and accelerating his learning of a foraging task. …

Construction And Characterization Of Non-Toxic Bacterial Enterotoxins As Vaccine Adjuvants, Lavanya Vempati

Boise State University Theses and Dissertations

The development of adjuvants that can promote the delivery of purified subunit vaccines by mucosal routes, such as the nose or the mouth, is recognized as a top priority for vaccine research. The bacterial enterotoxins; cholera toxin (CT) and E.coli heat-labile toxin (LTI), have long been recognized as powerful adjuvants with the ability to stimulate specific immune responses to co-administered antigens when delivered to mucosal surfaces. Shiga toxin 1 (ST1) and pertussis toxin (PT) are structurally homologous bacterial toxins secreted by Escherichia coli 0157:H7 and Bordetella pertussis respectively. ST1 and PT also have reported adjuvant activity but it is less …

Movements Of A Juvenile Crowned Eagle (Harpyhaliaetus Coronatus) Tracked By Satellite Telemetry In Central Argentina, Vicente Urios, Maria Pilar Donat-Torres, Marc J. Bechard, Miguel Ferrer

Biology Faculty Publications and Presentations

Background: A juvenile Crowned Eagle was tagged at its nest with a satellite transmitter. The Crowned Eagle (Harpyhaliaetus coronatus) is one of the most unknown raptor species from the American continent. Their current distribution ranges from central Brazil to central Argentina, with a total population of 350–1500 individuals across this large area, being thus largely fragmented.

Results: During the three years of tracking the bird concentrated its movements in a range spanning for 12845 km², but concentrating mainly in four smaller areas accounting for 3073 km². The locations were recorded mainly over shrubland habitats …

Factors Associated With Flammulated Owl And Northern Saw-Whet Owl Occupancy In Southern Idaho, Micah N. Scholer, Matthias Leu, James R. Belthoff

Biology Faculty Publications and Presentations

Spatially explicit models depicting species occupancy offer a useful conservation tool for land managers. Using occurrence data collected in 2009 and 2010 from the Boise National Forest, Idaho, we developed distribution models for Flammulated Owls (Psiloscops flammeolus) and Northern Saw-whet Owls (Aegolius acadicus) to explore associations between habitat factors and owl occupancy. We then spatially applied these models in a Geographic Information System. We considered land cover and topographic variables at three spatial scales: 0.4-km, 1-km, or 3-km-radius plots centered on point-count locations (n  =  150) with resolution of land covers at 30 m. Flammulated …

Diet Of A Cryptic But Widespread Invader, The Oriental Weatherfish (Misgurnus Anguillicaudatus) In Idaho, Usa, Alexander N. Urquhart, Peter Koetsier

Biology Faculty Publications and Presentations

The oriental weatherfish (Misgurnus anguillicaudatus) is invasive to 13 U.S. states and at least 9 other countries. Although this cryptic fish species is widespread, very little is known regarding its ecology. We used baited minnow traps to collect 237 individuals from water bodies connected to the Boise River, Idaho. To determine the diet of the fish within this invasive population, we dissected and examined stomach contents of the sampled fish. Most of the stomachs (158 of 237) were empty, and 42 contained only the trap bait. However, 37 contained natural food items. Gravimetric analysis, frequency of occurrence, and …

Influence Of Wildfire Disturbance And Post-Fire Seeding On Vegetation And Insects In Sagebrush Habitats, Ashley T. Rohde

Boise State University Theses and Dissertations

Disturbance events alter community composition and structure because of differences in resistance and resilience of individual taxa, changes in habitat resulting in colonization by new taxa and alteration of biotic interaction patterns. Recent changes in disturbance types, frequencies and intensities caused by anthropogenic activities may further alter community composition and structure if these disturbances exceed the tolerances or adaptations of some taxa. In sagebrush steppe habitats of the western United States, wildfire is the current dominant disturbance type, burning millions of hectares annually. Further, up to 90% of sagebrush-steppe ecosystems are affected by anthropogenic influences such as invasive species. Post-fire …

The First Successful Use Of A Low Stringency Familial Match In A French Criminal Investigation, Emmanuel Pham-Hoai, Frank Crispino, Greg Hampikian

Biology Faculty Publications and Presentations

We describe how a very simple application of familial searching resolved a decade-old, high-profile rape/murder in France. This was the first use of familial searching in a criminal case using the French STR DNA database, which contains approximately 1,800,000 profiles. When an unknown forensic profile (18 loci) was searched against the French arrestee/offender database using CODIS configured for a low stringency search, a single low stringency match was identified. This profile was attributed to the father of the man suspected to be the source of the semen recovered from the murder victim Elodie Kulik. The identification was confirmed using Y-chromosome …

Immunogenicity Of A West Nile Virus Diii-Cholera Toxin A2/B Chimera After Intranasal Delivery, Juliette K. Tinker, Jie Yan, Reece J. Knippel, Panos Panayiotou, Kenneth A. Cornell

Biology Faculty Publications and Presentations

West Nile virus (WNV) causes potentially fatal neuroinvasive disease and persists at endemic levels in many parts of the world. Despite advances in our understanding of WNV pathogenesis, there remains a significant need for a human vaccine. The domain III (DIII) region of the WNV envelope protein contains epitopes that are the target of neutralizing antibodies. We have constructed a chimeric fusion of the non-toxic cholera toxin (CT) CTA₂/B domains to DIII for investigation as a novel mucosally-delivered WNV vaccine. Purification and assembly of the chimera, as well as receptor-binding and antigen delivery, were verified by western blot, …

Temporal And Spatial Variation Of Nitrogen In Wyoming Big Sagebrush, Stephanie Williams, Jordan Nobler, Kristina Gehlken, Janet Rachlow, Lisa Shipley

College of Arts and Sciences Presentations

Sagebrush steppe systems represent one of North America’s greatest conservation challenges. Shrinking habitat and declining animal populations have prompted researchers to fill gaps in our understanding of the ecology of this system, particularly at a scale relevant to individual animals. What animals eat and why are fundamental questions linked to habitat quality and use. We investigated the temporal and spatial heterogeneity of the dietary quality of food to better understand habitat use by a sagebrush specialist, the pygmy rabbit (Brachylagus idahoensis). Within a foraging patch, pygmy rabbits selectively browse on individual Wyoming big sagebrush (Artemisia tridentata wyomingensis) that are …

Stüve-Wiedemann Syndrome: Lifr And Associated Cytokines In Clinical Course And Etiology, Dawn Mikelonis, Cheryl L. Jorcyk, Ken Tawara, Julia Thom Oxford

Biology Faculty Publications and Presentations

Stüve-Wiedemann syndrome (STWS; OMIM #610559) is a rare bent-bone dysplasia that includes radiologic bone anomalies, respiratory distress, feeding difficulties, and hyperthermic episodes. STWS usually results in infant mortality, yet some STWS patients survive into and, in some cases, beyond adolescence. STWS is caused by a mutation in the leukemia inhibitory factor receptor (LIFR) gene, which is inherited in an autosomally recessive pattern. Most LIFR mutations resulting in STWS are null mutations which cause instability of the mRNA and prevent the formation of LIFR, impairing the signaling pathway. LIFR signaling usually follows the JAK/STAT3 pathway, and is initiated by …

Apolipoprotein E Pathology In Vascular Dementia, Troy T. Rohn, Ryan J. Day, Colin B. Sheffield, Alexander J. Rajic, Wayne W. Poon

Biology Faculty Publications and Presentations

Vascular dementia (VaD) is the second most common form of dementia and is currently defined as a cerebral vessel vascular disease leading to ischemic episodes. Apolipoprotein E (apoE) gene polymorphism has been proposed as a risk factor for VaD, however, to date there are few documented post-mortem studies on apoE pathology in the VaD brain. To investigate a potential role for the apoE protein, we analyzed seven confirmed cases of VaD by immunohistochemistry utilizing an antibody that specifically detects the amino-terminal fragment of apoE. Application of this antibody, termed N-terminal, apoE cleavage fragment (nApoECF) revealed consistent labeling within neurofibrillary tangles …

Morphologic And Molecular Description Of Metopus Fuscus Kahl From North America And New Rdna Sequences From Seven Metopids (Armophorea, Metopidae), William A. Bourland, Laura Wendell, Greg Hampikian

Biology Faculty Publications and Presentations

Most species in the large ciliate genus Metopus Claparède & Lachmann, 1858 lack detailed descriptions based on modern morphologic and molecular methods. This lack of data for the vast majority of species hampers application of a morphospecies approach to the taxonomy of Metopus and other armophorids. In this report we redescribe the large species, Metopus fuscus Kahl, 1927 based on in vivo observation, silver impregnation, scanning electron microscopy, and single-cell 18S rDNA sequencing of a freshwater North American (Idaho) population. Metopus fuscus invariably has a perinuclear envelope of endosymbiotic bacteria not found in other species. Unlike the original description of …

Regional Distribution Shifts Help Explain Local Changes In Wintering Raptor Abundance: Implications For Interpreting Population Trends, Neil Paprocki, Julie A. Heath, Shawn J. Novak

Biology Faculty Publications and Presentations

Studies of multiple taxa across broad-scales suggest that species distributions are shifting poleward in response to global climate change. Recognizing the influence of distribution shifts on population indices will be an important part of interpreting trends within management units because current practice often assumes that changes in local populations reflect local habitat conditions. However, the individual- and population-level processes that drive distribution shifts may occur across a large, regional scale and have little to do with the habitats within the management unit. We examined the latitudinal center of abundance for the winter distributions of six western North America raptor species …

Immunolocalization Of An Amino-Terminal Fragment Of Apolipoprotein E In The Pick's Disease Brain, Troy T. Rohn, Ryan J. Day, Lindsey W. Catlin, Raquel J. Brown, Alexander J. Rajic, Wayne W. Poon

Biology Faculty Publications and Presentations

Although the risk factor for apolipoprotein E (apoE) polymorphism in Alzheimer's disease (AD) has been well described, the role that apoE plays in other neurodegenerative diseases, including Pick's disease, is not well established. To examine a possible role of apoE in Pick's disease, an immunohistochemical analysis was performed utilizing a novel site-directed antibody that is specific for an amino-terminal fragment of apoE. Application of this antibody, termed the amino-terminal apoE cleavage fragment (nApoECF) antibody, consistently labeled Pick bodies within area CA1 of the hippocampus in 4 of the 5 cases examined. Co-localization of the nApoECF antibody with PHF-1, a general …

Comparative Genome Analysis Of Ciprofloxacin-Resistant Pseudomonas Aeruginosa Reveals Genes Within Newly Identified High Variability Regions Associated With Drug Resistance Development, Hsun-Cheng Su, Jainab Khatun, Dona M. Kanavy, Morgan C. Giddings

Biology Faculty Publications and Presentations

The alarming rise of ciprofloxacin-resistant Pseudomonas aeruginosa has been reported in several clinical studies. Though the mutation of resistance genes and their role in drug resistance has been researched, the process by which the bacterium acquires high-level resistance is still not well understood. How does the genomic evolution of P. aeruginosa affect resistance development? Could the exposure of antibiotics to the bacteria enrich genomic variants that lead to the development of resistance, and if so, how are these variants distributed through the genome? To answer these questions, we performed 454 pyrosequencing and a whole genome analysis both before and after …

Protargol Synthesis: An In-House Protocol, Xuming Pan, William A. Bourland, Weibo Song

Biology Faculty Publications and Presentations

The protargol staining method has proved to be indispensable for revealing the cellular structures of a variety of protozoa, especially the flagellates and ciliates. Protargol provides permanent stains of a variety of cellular structures: nuclei, extrusomes, basal bodies, and microfilamentous constituents of cells. Together with the older silver nitrate methods, protargol impregnations have provided the basis for the detailed descriptions of nearly all ciliates to date. The performance of commercially available preparations has varied widely. Recently, suppliers have stopped stocking the effective forms of protargol resulting in a worldwide shortage. Thus, it has become necessary for protistologists to explore on-site …

Serdemetan Antagonizes The Mdm2-Hif1Α Axis Leading To Decreased Levels Of Glycolytic Enzymes, Allan R. Albig

Biology Faculty Publications and Presentations

Serdemetan (JNJ-26854165), an antagonist to Mdm2, was anticipated to promote the activation of p53. While regulation of p53 by Mdm2 is important, Mdm2 also regulates numerous proteins involved in diverse cellular functions. We investigated if Serdemetan would alter the Mdm2-HIF1α axis and affect cell survival in human glioblastoma cells independently of p53. Treatment of cells with Serdemetan under hypoxia resulted in a decrease in HIF1α levels. HIF1α downstream targets, VEGF and the glycolytic enzymes (enolase, phosphoglycerate kinase1/2, and glucose transporter 1), were all decreased in response to Serdemetan. The involvement of Mdm2 in regulating gene expression of glycolytic enzymes raises …

Autism As The Early Closure Of A Neuroplastic Critical Period Normally Seen In Adolescence, Julia Marie Berger, Troy T. Rohn, Julia Thom Oxford

Biology Faculty Publications and Presentations

The most severe cases of autism are diagnosed by extreme social dysfunction and other behavioral abnormalities. A number of genetic studies have been conducted to correlate behavioral phenotypes to genetic dysfunctions, but no “autism gene” has yet been discovered. In addition, environmental factors have been found to influence the development of autistic traits with high probability. This review will examine the role of a shortened period of neuroplasticity as a unifying feature of the autistic phenotype. The neuroplastic period of interest normally extends into adolescence, allowing for neural integration and the development of language and social skills. Early closure of …

Impacts Of An Ethanol-Blended Fuel Release On Groundwater And Fate Of Produced Methane: Simulation Of Field Observations, Kevin P. Feris

Biology Faculty Publications and Presentations

[1] In a field experiment at Vandenberg Air Force Base (VAFB) designed to mimic the impact of a small-volume release of E10 (10% ethanol and 90% conventional gasoline), two plumes were created by injecting extracted groundwater spiked with benzene, toluene, and o-xylene, abbreviated BToX (no-ethanol lane) and BToX plus ethanol (with-ethanol lane) for 283 days. We developed a reactive transport model to understand processes controlling the fate of ethanol and BToX. The model was calibrated to the extensive field data set and accounted for concentrations of sulfate, iron, acetate, and methane along with …

Local Recruitment And Natal Dispersal Distances Of American Kestrels, Karen Steenhof, Julie A. Heath

Biology Faculty Publications and Presentations

Variation in recruitment patterns and dispersal behavior can have important consequences for population viability, genetic structure, and rates of evolutionary change. From 1992 to 2006 we studied a marked population of American Kestrels (Falco sparverius) nesting in boxes in southwestern Idaho to identify factors that affect local recruitment and natal dispersal distances. A low proportion (4%) of locally produced kestrels (n = 2180) returned to nest in study area boxes. Offspring of locally produced individuals were 3.1 times more likely to return than offspring of parents that did not hatch in study area boxes and, independent of …

A Framework For Understanding Noise Impacts On Wildlife: An Urgent Conservation Priority, Clinton D. Francis, Jesse R. Barber

Biology Faculty Publications and Presentations

Anthropogenic noise is an important environmental stressor that is rapidly gaining attention among biologists, resource managers, and policy makers. Here we review a substantial literature detailing the impacts of noise on wildlife and provide a conceptual framework to guide future research. We discuss how several likely impacts of noise exposure have yet to be rigorously studied and outline how behavioral responses to noise are linked to the nature of the noise stimulus. Chronic and frequent noise interferes with animals’ abilities to detect important sounds, whereas intermittent and unpredictable noise is often perceived as a threat. Importantly, these effects can lead …

The River Otter In Idaho: Reproductive And Population Parameters And Liver Concentrations Of Environmental Contaminants, Gina Diggs

Boise State University Theses and Dissertations

To obtain current data on the North American river otter (Lontra canadensis) population in Idaho, licensed trappers were mandated to surrender river otter carcasses through provisions of the Idaho Department of Fish and Game’s mandatory harvest report. Throughout the 2002-2003 and 2003-2004 trapping seasons, 237 river otter carcasses were collected. Necropsies were performed to assess age and sex, general body condition, reproductive rates, and concentrations of environmental contaminants in the livers. Reproductive rates were determined by counting corpora lutea and blastocysts in female river otters. Livers were dissected and concentrations of environmental contaminants were determined for the following …

Impacts Of Wind Energy Development On Breeding Buteo Hawks In The Columbia Plateau Ecoregion, Patrick Shea Kolar

Boise State University Theses and Dissertations

Post-construction raptor fatality and nest monitoring is typically conducted at wind energy projects nationwide. However, pre- and post-construction surveys may fail to locate all breeding pairs and most studies at individual wind projects lack the necessary sample size or survey design to assess the effects of turbines on nesting raptors after construction. To address these potential issues, I used an information-theoretic approach to examine the influence of multiple spatial and temporal variables on reproductive success, post-fledging survival, and the distribution of breeding pairs from three sympatric Buteo species in the Columbia Plateau Ecoregion (CPE). Although the probability of detecting breeding …

Proteolytic Cleavage Of Apolipoprotein E4 As The Keystone For The Heightened Risk Associated With Alzheimer’S Disease, Troy T. Rohn

Biology Faculty Publications and Presentations

Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by microscopic lesions consisting of beta-amyloid plaques and neurofibrillary tangles (NFTs). The majority of cases are defined as sporadic and are likely caused by a combination of both genetic and environmental factors. Of the genetic risk factors identified, the 34 kDa protein, apolipoprotein (apo) E4, is of significant importance as APOE4 carriers account for 65%–80% of all AD cases. Although apoE4 plays a normal role in lipoprotein transport, how it contributes to AD pathogenesis is currently unknown. One potential mechanism by which apoE4 contributes to disease risk is its propensity to …

Increase In Acc Oxidase Levels And Activities During Paradormancy Release Of Leafy Spurge (Euphorbia Esula) Buds, Wun S. Chao, Marcelo Serpe, Jeffrey C. Suttle, Ying Jia

Biology Faculty Publications and Presentations

The plant hormone ethylene is known to affect various developmental processes including dormancy and growth. Yet, little information is available about the role of ethylene during paradormancy release in underground adventitious buds of leafy spurge. In this study, we examined changes in ethylene evolution and the ethylene biosynthetic enzyme ACC oxidase following paradormancy release (growth induction). Our results did not show an obvious increase in ethylene during bud growth. However, when buds were incubated with 1 mM ACC, ethylene levels were higher in growing than non-growing buds, suggesting that the levels of ACC oxidase increased in growing buds. Real-time qPCR …

Examining New Phylogenetic Markers To Uncover The Evolutionary History Of Early-Diverging Fungi: Comparing Mcm7, Tsr1 And Rrna Genes For Single- And Multi-Gene Analyses Of The Kickxellomycotina, Eric D. Tretter, E. M. Johnson, Yan Wang, Prasanna Kandel, Merlin M. White

Biology Faculty Publications and Presentations

The recently recognised protein-coding genes MCM7 and TSR1 have shown significant promise for phylogenetic resolution within the Ascomycota and Basidiomycota, but have remained unexamined within other fungal groups (except for Mucorales). We designed and tested primers to amplify these genes across early-diverging fungal clades, with emphasis on the Kickxellomycotina, zygomycetous fungi with characteristic flared septal walls forming pores with lenticular plugs. Phylogenetic tree resolution and congruence with MCM7 and TSR1 were compared against those inferred with nuclear small (SSU) and large subunit (LSU) rRNA genes. We also combined MCM7 and TSR1 data with the rDNA data to …

Bromus Tectorum Litter Alters Photosynthetic Characteristics Of Biological Soil Crusts From A Semiarid Shrubland, Marcelo D. Serpe, Eric Roberts, David J. Eldridge, Roger Rosentreter

Biology Faculty Publications and Presentations

Invasion by the exotic annual grass Bromus tectorum has increased the cover and connectivity of fine litter in the sagebrush steppes of western North America. This litter tends to cover biological soil crusts, which could affect their metabolism and growth. To investigate this possible phenomenon, biological soil crusts dominated by either the moss Bryum argenteum or the lichen Diploschistes muscorum were covered with B.tectorum litter (litter treatment) or left uncovered (control treatment) and exposed to natural field conditions. After periods of five and ten months, we removed the litter and compared the photosynthetic performance of biological soil crusts …

Evaluating The Effects Of Fungicides And Other Pesticides On Non-Target Gut Fungi And Their Aquatic Insect Hosts, Emma R. Wilson

Boise State University Theses and Dissertations

Pesticides are widespread and have been long used to combat the attack and destruction of crops. Fungicides have been used to prevent the establishment of many fungal pathogens, yet little is known about the impacts of fungicides on non-target fungi. With these considerations, it was predicted that trichomycetes, or gut fungi, a group of symbiotic fungi associated with aquatic macroinvertebrates and other arthropods, might be a candidate system to study because of the intimate association with their hosts. Field and laboratory studies were initiated to assess non-target impacts of fungicides on gut fungi. Field surveys were conducted on four streams …

A Pharm-Ecological Perspective Of Terrestrial And Aquatic Plant-Herbivore Interactions, Jennifer Sorensen Forbey, M. Denise Dearing, Elisabeth M. Gross, Colin M. Orians, Erik E. Sotka, William J. Foley

Biology Faculty Publications and Presentations

We describe some recent themes in the nutritional and chemical ecology of herbivores and the importance of a broad pharmacological view of plant nutrients and chemical defenses that we integrate as “Pharm-ecology”. The central role that dose, concentration, and response to plant components (nutrients and secondary metabolites) play in herbivore foraging behavior argues for broader application of approaches derived from pharmacology to both terrestrial and aquatic plant-herbivore systems. We describe how concepts of pharmacokinetics and pharmacodynamics are used to better understand the foraging phenotype of herbivores relative to nutrient and secondary metabolites in food. Implementing these concepts into the field …

Human Disturbance And Stage-Specific Habitat Requirements Influence Snowy Plover Site Occupancy During The Breeding Season, Alyson F. Webber, Julie A. Heath, Richard A. Fischer

Biology Faculty Publications and Presentations

Habitat use has important consequences for avian reproductive success and survival. In coastal areas with recreational activity, human disturbance may limit use of otherwise suitable habitat. Snowy plovers Charadrius nivosus have a patchy breeding distribution along the coastal areas on the Florida Panhandle, USA. Our goal was to determine the relative effects of seasonal human disturbance and habitat requirements on snowy plover habitat use. We surveyed 303 sites for snowy plovers, human disturbance, and habitat features between January and July 2009 and 2010. We made multiple visits during three different sampling periods that corresponded to snowy plover breeding: pre-breeding, incubation, …