Biology Commons^™

Transcription Factor Expression Landscape In Drosophila Embryonic Cell Lines, Robert A. Drewell, Daniel Klonaros, Jacqueline Dresch

Biology

Background: Transcription factor (TF) proteins are a key component of the gene regulatory networks that control cellular fates and function. TFs bind DNA regulatory elements in a sequence-specific manner and modulate target gene expression through combinatorial interactions with each other, cofactors, and chromatin-modifying proteins. Large-scale studies over the last two decades have helped shed light on the complex network of TFs that regulate development in Drosophila melanogaster. Results: Here, we present a detailed characterization of expression of all known and predicted Drosophila TFs in two well-established embryonic cell lines, Kc167 and S2 cells. Using deep coverage RNA sequencing approaches we …

Effects Of Roadways On Seasonal Movement Strategies And Mate Location Success In An Imperiled Pit Viper (Crotalus Horridus), Elizabeth J. Noble

Graduate Research Showcase

A detailed understanding of animal movement behavior is fundamental to effective conservation and management. Within populations, a diversity of movement strategies can be displayed in search of critical resources, and these strategies are influenced by multiple interacting factors related to individuals and the environment. Mating partners are one critical resource that often serves as a prominent selective force shaping movement during mating seasons. For many large-bodied snakes, such as pit vipers (Viperidae: Crotalinae), male mate- searching movements are the primary determinant of mate location success. During this time, males incur significant risks associated with elevated movement. In an increasingly human- …

Application Of Criminology In Urban Ecology And Evolution: Routine Activity Theory And Field Equipment Disappearance Dynamics, Ignacy Stadnicki, Michela Corsini, Marta Szulkin

Biology Faculty Publications and Presentations

Research in urban ecology and evolution relies on the use of deployable scientific equipment. If left unattended in the field, scientific equipment may be prone to vandalism and theft, especially in the urban space. Here, we empirically applied a theory derived from the field of criminology, specifically the Routine Activity Theory (RAT) framework, to predict disappearance rates of scientific equipment in an ongoing urban ecology research project. First, we tested a routinely applied method of equipment protection – labelling – and investigated whether equipment disappearance varied with label information content and message tone. Second, we examined whether equipment attributes (price, …

How Will Climate Change-Induced Increases In Wildfire Frequency Affect Soil Microbial Communities?, Bahar Saadaie Jahromi

Waldo Library Student Exhibits

As climate change progresses, wildfires will become more common, increasing their impact on soil microbial ecosystems. This research examines how increased fire frequency affects soil microbial communities and whether effects differ for communities associated with different tree species. Soil samples were collected from under five individuals of four tree species within each of four 1-ha plots that had experienced fire from 24 to 111 years ago. Samples were analyzed for microbial DNA using next generation sequencing techniques in order to explore the influence of fire return interval and tree species on microbial diversity and community structure. Correlations were also examined …

Effects Of Light Intensity And Predator Chemical Cues On Juvenile And Adult Daphnia Magna Diel Vertical Migration, Mira Johnson

Lawrence University Honors Projects

Many species of zooplankton exhibit the predator avoidance behavior called diel vertical migration (DVM). During typical DVM, zooplankton ascend to surface waters during the night and descend to deeper water during the day where predation by visual predators is reduced due to lower light levels (Goldman and Horne 1983; Wetzel 1983). DVM is highly variable and the degree to which it is induced can fluctuate based on factors such as zooplankton size, changes in light intensity, and predator chemical cues (Van Gool and Ringelberg 1995, cited in Weber and Noordwijk 2002). Although DVM has been well studied using the genus …

Oil Identification Of Harp Seal And Other Select Marine Mammals, Meghan Pinedo, Deborah A. Duffield, Dalin N. D’Alessandro, Erin R. Price, Edgard O. Espinoza

Biology Faculty Publications and Presentations

Because of the rich omega-3 fatty acids content, harp seal (Pagophilus groenlandicus) oil is a popular supplement that is packaged as pills in Canada and sold for medicinal purposes, although this practice is banned in the United States. Due to US regulations, it is important to be able to distinguish between fish oil and seal oil, but the taxonomic determination of oils provenance has been a difficult problem to solve. In this study, Direct Analysis in Real Time time-of-flight mass spectrometry (DART TOFMS) was used to analyze the chemotypes of blubber samples collected from seven species of …

The Value Of Support: Stem Intervention Programs Impact Student Persistence And Belonging., Erin E. Shortlidge, Mackenzie J. Gray, Suzanne Estes, Emma C. Goodwin

Biology Faculty Publications and Presentations

In response to unwaveringly high attrition from STEM pathways, STEM Intervention Programs (SIPs) support STEM students in effort to increase retention. Using mixed methods (survey and focus groups), we studied students at one university who were either supported or unsupported by SIPs to understand how students may differ in experiences believed to contribute to STEM persistence. We evaluated: sense of belonging, scientific self-efficacy, scientific community values, scientific identity, and STEM involvement. The enrollment status of students two and a half years postsurvey was also tracked. SIP students reported significantly higher science identity and sense of belonging and were more involved …

Structural Heterogeneity Predicts Ecological Resistance And Resilience To Wildfire In Arid Shrublands, Andrii Zaiats, Megan E. Cattau, David S. Pilliod, Rongsong Liu, Patricia Kaye T. Dumandan, Ahmad Hojatimalekshah, Donna M. Delparte, T. Trevor Caughlin

Biology Faculty Publications and Presentations

Context Dynamic feedbacks between physical structure and ecological function drive ecosystem productivity, resilience, and biodiversity maintenance. Detailed maps of canopy structure enable comprehensive evaluations of structure–function relationships. However, these relationships are scale-dependent, and identifying relevant spatial scales to link structure to function remains challenging.

Objectives We identified optimal scales to relate structure heterogeneity to ecological resistance, measured as the impacts of wildfire on canopy structure, and ecological resilience, measured as native shrub recruitment. We further investigated whether structural heterogeneity can aid spatial predictions of shrub recruitment.

Methods Using high-resolution imagery from unoccupied aerial systems (UAS), we mapped structural heterogeneity across …

On The Importance Of Sequence Alignment Inspections In Plastid Phylogenomics – An Example From Revisiting The Relationships Of The Water-Lilies, Jessica A. Roestel, John H. Wiersema, Robert K. Jansen, Thomas Borsch, Michael Gruenstaeudl

Biological Sciences Faculty Publications

The water-lily clade represents the second earliest-diverging branch of angiosperms. Most of its species belong to Nymphaeaceae, of which the “core Nymphaeaceae”—comprising the genera Euryale, Nymphaea and Victoria—is the most diverse clade. Despite previous molecular phylogenetic studies on the core Nymphaeaceae, various aspects of their evolutionary relationships have remained unresolved. The length-variable introns and intergenic spacers are known to contain most of the sequence variability within the water-lily plastomes. Despite the challenges with multiple sequence alignment, any new molecular phylogenetic investigation on the core Nymphaeaceae should focus on these noncoding plastome regions. For example, a new plastid phylogenomic study on …

Autism Risk Gene Cul3 Alters Neuronal Morphology Via Caspase-3 Activity In Mouse Hippocampal Neurons, Qiang-Qiang Xia, Anju Singh, Jing Wang, Zhong Xin Xuan, Jeffrey D. Singer, Craig M. Powell

Biology Faculty Publications and Presentations

Autism Spectrum Disorders (ASDs) are neurodevelopmental disorders (NDDs) in which children display differences in social interaction/communication and repetitive stereotyped behaviors along with variable associated features. Cul3, a gene linked to ASD, encodes CUL3 (CULLIN-3), a protein that serves as a key component of a ubiquitin ligase complex with unclear function in neurons. Cul3 homozygous deletion in mice is embryonic lethal; thus, we examine the role of Cul3 deletion in early synapse development and neuronal morphology in hippocampal primary neuronal cultures. Homozygous deletion of Cul3 significantly decreased dendritic complexity and dendritic length, as well as axon formation. Synaptic spine density significantly …

The Effect Of Temperature On The Invasive Asian Needle Ant, Kaylee Mulligan '25

Biology Student Scholarship

The Asian Needle Ant (Brachyponera chinensis) is an invasive species currently spreading on the Eastern Coast of the United States. They were first reported around the 1930s in Florida and have since migrated to Massachusetts. As an invasive species, this pest originating from Asia has a negative impact on environment ecosystems and other species of ants. Asian Needle Ants displace resident ant colonies, taking over nesting sites by consuming food sources and decreasing the amount of space other ant colonies can use. They are also known to have a sting that contains venom more likely to result in …

Sea Surface Temperature Influence On Tropical Storms And Hurricanes, Elise Leroux '26

Biology Student Scholarship

Hurricanes are both dangerous and difficult to predict because of the various factors that influence the size and intensity of storms, where sea surface temperature (SST) is one of the most relevant variables. As temperatures are increasingly rising due to climate change, the effect on storm size, intensity, and frequency is going to be greater. There have been many studies how individual storms are affected, but less so on the overall frequency of hurricanes. This study assesses the number of hurricanes each year in comparison to the SST and the average wind speed of each hurricane. I predict that years …

Gdp Effects On Bottlenose Dolphins Within The Gulf Of Mexico, Kathleen Kelly '24

Biology Student Scholarship

Bottlenose dolphins (Tursiops truncatus) are highly sensitive to environmental changes and anthropogenic stressors. Making them important indicators of marine ecosystem health. The Gulf of Mexico sustains diverse marine ecosystems and coastal communities. However, rapid industrialization, urbanization, and coastal development have altered marine habitats and increased anthropogenic pressures on marine wildlife. While various factors contribute to these strandings, the potential influence of socio-economic factors, particularly Gross Domestic Product (GDP) disparities among Gulf Coast counties, remains underexplored. This number (GDP) is influenced by human activities such as fisheries, shipping, and tourism, all of which directly or indirectly could affect marine …

Michigan Water Supply: Analysis Of Health Violations, Gabriela Guzman Reyes '24

Biology Student Scholarship

The presence of toxins in water sources poses a significant public health challenge. In Michigan, this issue has garnered widespread attention due to its adverse effects on human health and well-being. The contaminants found in water, including but not limited to lead, industrial pollutants, and agricultural runoff, stem from a variety of sources such as aging infrastructure, industrial activities, and agricultural practices. News articles and Government officials have reported the adverse affects and impact toxin exposure has on Michigan residents. However, there is little research examining the relationship between water quality violations and socioeconomic factors like poverty levels. I predict …

The Effect Sea Temperature Has On Coral Reef Health In The Great Barrier Reef, Elizabeth Glasspool '26

Biology Student Scholarship

Coral reef bleaching can happen for many different reasons including storms, disease, sediment and salinity changes, and of change in climate. Mass bleaching is typically a cause of increasing sea temperature in a certain area. Mass bleaching typically kills off many different species of coral. This is detrimental to a large portion of biodiversity, especially in a marine atmosphere. In 2016, records show that it is a record high ocean temperature which led to widespread coral bleaching on Australian reefs. This was part of the third global bleaching event declared by the National Oceanic and Atmospheric Administration (NOAA). At the …

The Effect Of Integrated Pest Management On Nutrition Of Bee Collected Pollen, Kaitlyn Bresnahan '25

Biology Student Scholarship

Honey bees are the world’s most commercially valuable pollinators, and they face many threats. The greatest challenge facing honey bees is Varroa mites, parasitic mites that feed on bees’ fat bodies, killing bees and spreading diseases. While chemical treatments are available to stop the spread of mites, these treatments are insecticides, which are not ideal to put in a hive of insects. A popular organic method of mite management is using drone comb. Drone brood is preferentially chosen by mites for ovipositing and development because of its larger size and longer development time, so removing drone brood can drastically reduce …

The Effect Of Population Density On Harmful Algal Blooms In Southwest Florida, Emma Barry '25

Biology Student Scholarship

Karenia brevis is a species of algae native to the Gulf of Mexico. Times when its growth becomes uncontrollable are called harmful algal blooms (formerly called “red tide events”), and they are often caused by increased nutrients in the water. These excessive nutrients come from watersheds and runoff that contain anthropogenic materials like fertilizer, and the phosphate and nitrogen in fertilizers catalyze the growth of algae. As population density in Florida increases, fertilizer runoff increases as well. Harmful algal bloom events are dangerous to both humans and the environment. Thick layers of algal growth on the water’s surface create “dead …

Whale Harvest Through Time: Unveiling The Hemispheric Contrasts In Whaling, Laura Mooney '24

Biology Student Scholarship

Whales have been long known for their oils and rich blubber which has been a dish in many countries in both the Northern and Southern hemispheres. Some species were being hunted to near extinction. The earliest whaling began in 3000BC by Native American but commercial whaling began by the Basques in the 17th century with a huge increase in the 18th,19th and 20th centuries. This study examines how whale harvest has changed over time in years between 1900 and 1999 in both the Northern and Southern hemispheres. I analyzed the data collected by Jr. Robert(2015) and looked to see if …

Ultraconserved Elements Based Phylogeny Of The New Zealand Mite Harvester Genus Rakaia, Zade Alafranji

Biology Honors Projects

New Zealand is home to a remarkable number of endemic taxa, some of which existed on the archipelago before the breakup of Gondwana. The mite harvesters (suborder Cyphophthalmi), tiny arachnids which dwell in forest leaf litter and caves, are one such group. The mite harvester family Pettalidae exhibits a classic Gondwanan distribution, informative for describing ancient patterns of diversification. Within New Zealand, there are three genera of pettalids; our research focuses on the phylogeny of the most widespread and diverse of these: Rakaia. Through phylogenetic analysis, we provide a window into patterns of ancient diversification and infer historical biogeographic trends. …

West Coast Rock Lobster Resource Harvest Strategy, Department Of Primary Industries And Regional Development, Western Australia

Fisheries management papers

Harvest strategies for Western Australia’s (WA) aquatic resources are formal documents developed by the Department of Primary Industries and Regional Development (DPIRD, the Department) to support decision-making processes that ensure the outcomes are consistent with the principles of Ecologically Sustainable Development (ESD; Fletcher 2002a) and Ecosystem Based Fisheries Management (EBFM; Fletcher et al. 2012). Harvest strategies are a key component of all contemporary fishery management systems and a requirement for certification under the Marine Stewardship Council (MSC). The objectives of ESD are reflected in the objectives of the Fish Resources Management Act 1994 (FRMA) and the Aquatic Resources Management Act …

Parental Thermal Environment Controls The Offspring Phenotype In Brook Charr (Salvelinus Fontinalis): Insights From A Transcriptomic Study, Ghizlane Banousse, Eric Normandeau, Christine Semeniuk, Louis Bernatchez, Celine Audet

Great Lakes Institute for Environmental Research Publications

rook charr is a cold-water species which is highly sensitive to increased water temperatures, such as those associated with climate change. Environmental variation can potentially induce phenotypic changes that are inherited across generations, for instance, via epigenetic mechanisms. Here, we tested whether parental thermal regimes (intergenerational plasticity) and offspring-rearing temperatures (within-generational plasticity) modify the brain transcriptome of Brook charr progeny (fry stage). Parents were exposed to either cold or warm temperatures during final gonad maturation and their progeny were reared at 5 or 8 °C during the first stages of development. Illumina Novaseq6000 was used to sequence the brain transcriptome …

Gymnosperms Demonstrate Patterns Of Fine-Root Trait Coordination Consistent With The Global Root Economics Space, Jessica R. Langguth, Marcin Zadworny, Karl Andraczek, Marvin Lo, Newton Tran, Kelsey Patrick, Joanna Mucha, Luke Mccormack, Kevin E. Mueller

Biological, Geological, and Environmental Faculty Publications

Gymnosperms encompass a diverse group of mostly woody plants with high ecological and economic value, yet little is known about the scope and organization of fine-root trait diversity among gymnosperms due to the undersampling of most gymnosperm families and the dominance of angiosperm groups in recent syntheses. New and existing data were compiled for morphological traits (root diameter, length, tissue density, specific root length [SRL] and specific root area [SRA]), the architectural trait branching ratio, root nitrogen content [N] and mycorrhizal colonization. We used phylogenetic least squares regression and principal component analysis to determine trait-trait relationships and coordination across 66 …

Demography With Drones: Detecting Growth And Survival Of Shrubs With Unoccupied Aerial Systems, Peter J. Olsoy, Andrii Zaiats, Donna M. Delparte, Matthew J. Germino, Bryce A. Richardson, Anna V. Roser, Jennifer S. Forbey, Megan E. Cattau, T. Trevor Caughlin

Biology Faculty Publications and Presentations

Large-scale disturbances, such as megafires, motivate restoration at equally large extents. Measuring the survival and growth of individual plants plays a key role in current efforts to monitor restoration success. However, the scale of modern restoration (e.g., >10,000 ha) challenges measurements of demographic rates with field data. In this study, we demonstrate how unoccupied aerial system (UAS) flights can provide an efficient solution to the tradeoff of precision and spatial extent in detecting demographic rates from the air. We flew two, sequential UAS flights at two sagebrush (Artemisia tridentata) common gardens to measure the survival and growth of …

Investigating Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome Using Mitotalens, Megan Gusso

Honors Theses

This lab project explores the complexity of the mitochondrial genome by investigating large genomic repeats and their effect on flowering plants’ reproduction and viability. Mitochondria are the site of cellular respiration to create energy and contain their own DNA. This is counterintuitive because the byproducts of cellular respiration impose a threat of mutation for the stored DNA. The mitochondrial genome differs in size between animals and plants. Plant mitochondrial DNA is larger due to the presence of large, noncoding repeated sequences, and a large amount of DNA of unknown function. These sequences undergo high levels of recombination and rearrangement. The …

Exploring The Impact Of Subject Placement On Exam Questions​, Briana M. Craig, Jeremy L. Hsu

Student Scholar Symposium Abstracts and Posters

Quizzes and exams are crucial elements of undergraduate biology courses; however, little research has been done exploring the importance of the phrasing used in the questions on these assessments. Question framing has the potential to impact student performance and sense of belonging greatly. In this study, we conducted an experiment where we created three versions of the exact same question except for the framing for the experimental scenarios. One version was written with self-referential framing using the term “you,” simulating the student conducting the experiment; another version used classmate-referential framing, placing one of their peers in the experiment; the third …

Cross-Pollination: Building A Co-Taught Course To Examine Art And Sex Through The Lens Of Botany, Christopher T. Martine, Diamanda A. Zizis, Anna K. Kell

Faculty Journal Articles

Driven by overlapping interests in plants, art, and diversity in sex expression, Anna Kell (Department of Art and Art History) and Chris Martine (Department of Biology) developed a course that integrates the perspectives of a visual artist and a botanist. Art & Sex Through the Lens of Botany seeks to impart the importance of making connections across disciplines and the value of visual literacy across academic lines. The course introduces foundational concepts in each field and encourages students to integrate and explore these different systems of knowledge and their intersections. In addition to developing fluencies related to both general botany …

Spatiotemporal Gait Parameter Consistency Across Two Days Of Treadmill Walking In Stroke Survivors, Alejandro Aguirre Ramirez, Samantha N. Jeffcoat, Natalia Sanchez, Andrian Kuch

Student Scholar Symposium Abstracts and Posters

Retraining impaired gait is essential in post-stroke recovery. This project aims to determine between day variability of spatiotemporal gait measures in treadmill walking post-stroke to differentiate between changes due to the intervention and measurement errors due to between day test-retest variability. Six individuals post-stroke performed a two-minute walk test at a self-selected speed (SSS) for two consecutive days. SSS was assessed through the six-minute walk test. Reflective markers were placed on anatomical landmarks (van den Bogert et al. 2013). The heel markers were used for step detection. We calculated spatiotemporal parameters: stride length, stride time, step length, stance time, and …

Season Of Grazing Interacts With Soil Texture, Selecting For Associations Of Biocrust Morphogroups, Lea A. Condon, Roger Rosentreter, Kari E. Veblen, Peter S. Coates

Biology Faculty Publications and Presentations

Livestock grazing, a widespread land use in semi-arid systems, is often placed in opposition to the perpetuation of biological soil crusts (“biocrusts”: lichens, mosses, and algal crusts including cyanobacteria) that live on the soil surface and provide ecosystem functions. The composition of biocrusts and vascular plants varies with climate, soils, and disturbance. In general, ruderal mosses and light algal crusts make up greater proportions of biocrusts in the presence of disturbance, although morphogroups of biocrusts respond differently to various disturbances. It is unknown if there are scenarios under which grazing can occur and ruderal components of biocrust could be maintained. …

Revealing Nitrate Uptake And Dispersion Dynamics Using High-Frequency Sensors And Two-Dimensional Modeling In A Large River System, Amirreza Zarnaghsh, Michelle Kelly, Amy Burgin, Admin Husic

Michigan Tech Publications, Part 2

Nitrate pollution of water bodies is a critical issue in many parts of the world because of its negative effects on aquatic ecosystem and human health. Effective management of pollution, such as the continuous or instantaneous release from point-sources, requires an understanding – with high spatial and temporal resolution – of how nitrate is dispersed and cycled within rivers. Nitrate sensing data show promise for this purpose, but their integration into numerical models is scarce; thus, questions remain regarding the necessary spatial grid size and temporal resolution required to resolve sensor readings. In this study, we developed an unsteady two-dimensional …

Ecological Effects And Ethics Of A Species Range Shift To High Elevation Ponds, Ava Ardito

Honors College

As temperatures warm, the climate crisis is having a significant influence on species across the world and altering their distributions. Colorado's high elevation ponds have recently observed a species range shift, traveling to higher altitudes than they have previously inhabited. The Mexican Cut Nature Preserve near the Rocky Mountain Biological Laboratory (RMBL) in Gothic, CO, is home to a prominent caddisfly Limnephilus externus, but recently a new range-shifting species, Nemotaulius hostilis, has appeared. Caddisflies play an essential role in nutrient cycling and putting energy back into the food web. The range-shifting species is known to excrete high rates of phosphorus …